Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.707 [Categoria DeCS]
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[PMID]:29431062
[Au] Autor:Amzar N; Iqbal M
[Ad] Endereço:Biotechnology Research Institute, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.
[Ti] Título:The Hepatoprotective Effect of Clidemia hirta against Carbon Tetrachloride (CCl4)-Induced Oxidative Stress and Hepatic Damage in Mice.
[So] Source:J Environ Pathol Toxicol Oncol;36(4):293-307, 2017.
[Is] ISSN:2162-6537
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Liver diseases still represents a major health burden worldwide. Moreover, medicinal plants have gained popularity in the treatment of several diseases, including liver disease. Clidemia hirta possesses many medicinal properties for healing several diseases and for health maintenance. However, the hepatoprotective effects and antioxidative potential of C. hirta have not been fully investigated. In the present study, we evaluated the hepatoprotective and antioxidative potential of C. hirta against carbon tetrachloride (CCl4)-induced liver injuries and oxidative damage in a murine model. Various biochemical changes associated with liver damage and oxidative stress were measured. The mice were pretreated for 14 consecutive days with aqueous extract of C. hirta at selected doses (150 mg/kg body weight [b.w.], 300 mg/kg b.w. and 600 mg/kg b.w.) followed by two doses of CCl4 (1.0 mL/kg b.w.) orally on days 14 and 15. All animals were sacrificed 24 hours after the last dose of CCl4 or saline. Blood and liver tissues were taken quickly for biochemical and histopathological studies to assess the derangement in the functioning of liver. The development of oxidative stress was observed through the escalation of hepatic lipid peroxidation, depletion of glutathione, and reduced antioxidant enzymes (glutathione peroxidase, glutathione reductase, catalase, glutathione S-transferase and quinone reductase). Hepatic damage was evaluated by measuring serum transaminase (ALT and AST). In addition, CCl4-induced hepatic damage was further evaluated using histopathological assessments. However, most of these changes were dependently ameliorated by the pretreatment of mice with a C. hirta dose. These results indicate that the hepatoprotective effect of C. hirta might be due to its antioxidant and free-radical scavenging properties.
[Mh] Termos MeSH primário: Fígado/efeitos dos fármacos
Melastomataceae
Estresse Oxidativo/efeitos dos fármacos
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Alanina Transaminase/sangue
Animais
Aspartato Aminotransferases/sangue
Tetracloreto de Carbono/toxicidade
Peroxidação de Lipídeos/efeitos dos fármacos
Fígado/metabolismo
Fígado/patologia
Masculino
Melastomataceae/química
Camundongos
Camundongos Endogâmicos BALB C
Extratos Vegetais/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); CL2T97X0V0 (Carbon Tetrachloride); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180213
[St] Status:MEDLINE
[do] DOI:10.1615/JEnvironPatholToxicolOncol.2017019824


  2 / 193 MEDLINE  
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[PMID]:28448467
[Au] Autor:Araújo JS; Chambó ED; Costa MAPC; Cavalcante da Silva SMP; Lopes de Carvalho CA; M Estevinho L
[Ad] Endereço:Centro de Ciências Agrárias, Ambientais e Biológicas, Universidade Federal do Recôncavo da Bahia, Cruz das Almas 44380-000, Bahia, Brazil. jucilenearaujo15@hotmail.com.
[Ti] Título:Chemical Composition and Biological Activities of Mono- and Heterofloral Bee Pollen of Different Geographical Origins.
[So] Source:Int J Mol Sci;18(5), 2017 Apr 27.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Recent research shows variations in pollen chemical constituents and, consequently, in their therapeutic properties. Mono and multifloral bee pollen extracts were investigated for antioxidant and enzyme inhibitory activity properties, phenolic compounds and fatty acid composition. Generally, spp. and multifloral extracts exhibited potent inhibitory activity against α-amylase, acetylcholinesterase, tyrosinase, lipoxygenase, lipase and hyaluronidase. On the other hand, spp. demonstrated higher antihemolytic activity. and spp. extracts exhibited important antioxidant properties in the different assays (ABTS, DPPH, ß-carotene/linoleic acid and reducing power). Moreover, these extracts had greater amounts of total phenols and flavonoids in comparison to others. The increase in antioxidant activity (decrease in EC values) was accompanied by an increase in the amount of total phenols in the extracts. The pollen extracts contained linoleic acid and α-linolenic acid as major fatty acids, followed by palmitic acid, and oleic acid. In this study, differences were observed in both chemical constituents and biological activities of the samples related to the geographical and botanical origin of bee pollen.
[Mh] Termos MeSH primário: Extratos Vegetais/química
Pólen/química
[Mh] Termos MeSH secundário: Acetilcolinesterase/química
Acetilcolinesterase/metabolismo
Animais
Antioxidantes/química
Antioxidantes/metabolismo
Abelhas
Cromatografia Gasosa
Cocos/química
Cocos/metabolismo
Eucalyptus/química
Eucalyptus/metabolismo
Ácidos Graxos/análise
Ácidos Graxos/química
Flavonoides/análise
Flavonoides/química
Melastomataceae/química
Melastomataceae/metabolismo
Monofenol Mono-Oxigenase/antagonistas & inibidores
Monofenol Mono-Oxigenase/metabolismo
Fenóis/análise
Fenóis/química
Extratos Vegetais/metabolismo
Análise de Componente Principal
alfa-Amilases/antagonistas & inibidores
alfa-Amilases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Fatty Acids); 0 (Flavonoids); 0 (Phenols); 0 (Plant Extracts); EC 1.14.18.1 (Monophenol Monooxygenase); EC 3.1.1.7 (Acetylcholinesterase); EC 3.2.1.1 (alpha-Amylases)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180122
[Lr] Data última revisão:
180122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE


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[PMID]:29198636
[Au] Autor:de Almeida CLB; Cechinel-Filho V; Boeing T; Mariano LNB; Silva LMD; Andrade SF; de Souza P
[Ad] Endereço:Programa de Pós-Graduação em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), Itajaí, SC 88302-901, Brazil.
[Ti] Título:Prolonged diuretic and saluretic effect of nothofagin isolated from Leandra dasytricha (A. Gray) Cogn. leaves in normotensive and hypertensive rats: Role of antioxidant system and renal protection.
[So] Source:Chem Biol Interact;279:227-233, 2018 Jan 05.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Although the acute diuretic effect of nothofagin has been recently demonstrated, its effects after dose-repeated treatment have not yet been explored. For that, male Wistar normotensive (NTR) and spontaneously hypertensive rats (SHR) were orally treated, once a day, with vehicle (VEH: distilled water; 1 ml/kg), hydrochlorothiazide (HCTZ; 10 mg/kg) or nothofagin (NOT; 1 mg/kg). The cumulative diuretic index and urinary electrolytes excretion were measured each 24 h. On the last day of the experiment (7th day), urine, blood and kidney samples were collected for biochemical and molecular analyzes. The urinary volume of both NTR and SHR were significantly increased with the treatment with NOT (from the second to the seventh day of treatment), with final values reaching an increase of 56% and 82%, respectively, when compared with VEH-treated group. This effect was associated with increased levels of urinary excretion of Na and Cl , without any changes on K excretion. None of the treatments modified urinary pH or density values. Importantly, neither the NOT nor the HCTZ caused any change in body weight following the dose-repeated treatment, and also did not provoke an electrolytic disturbance. Regarding the renal analyzes, when compared with the vehicle-treated NTR group, the activity of superoxide dismutase (SOD) and the reduced glutathione (GSH) levels in kidney homogenates of the SHR group were decreased, while the generation of lipid hydroperoxides were significantly increased. The daily treatment with NOT was able to restore the GSH levels and SOD activity, as well as reduced the lipoperoxidation in the kidney homogenates obtained from SHR animals. Finally, NOT significantly augmented the levels of nitrite, a marker of nitric oxide production, in the plasma obtained from SHR group when compared with the vehicle-treated only NTR. This study revealed the prolonged diuretic and saluretic effect of nothofagin in normotensive and hypertensive rats. Our data also showed the renal protective effects of nothofagin by the improvement of antioxidative capacity, as well as by the augmented bioavailability of plasma nitric oxide in the hypertensive group.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Chalconas/farmacologia
Hipertensão/tratamento farmacológico
Rim/efeitos dos fármacos
Melastomataceae/química
Folhas de Planta/química
[Mh] Termos MeSH secundário: Animais
Chalconas/uso terapêutico
Diuréticos/farmacologia
Hidroclorotiazida/farmacologia
Masculino
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Chalcones); 0 (Diuretics); 0 (nothofagin); 0J48LPH2TH (Hydrochlorothiazide)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180103
[Lr] Data última revisão:
180103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171205
[St] Status:MEDLINE


  4 / 193 MEDLINE  
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[PMID]:28421612
[Au] Autor:Tiwary BK; Ghosh R; Moktan S; Ranjan VK; Dey P; Choudhury D; Dutta S; Deb D; Das AP; Chakraborty R
[Ad] Endereço:Omics Laboratory, Department of Biotechnology, University of North Bengal, Siliguri, India.
[Ti] Título:Prospective bacterial quorum sensing inhibitors from Indian medicinal plant extracts.
[So] Source:Lett Appl Microbiol;65(1):2-10, 2017 Jul.
[Is] ISSN:1472-765X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:As virulence of many pathogenic bacteria is regulated by the phenomenon of quorum sensing (QS), the present study aimed to find the QS-inhibiting (QS-I) property (if any) in 61 Indian medicinal plants. The presence of QS-I compound in the leaf extract was evaluated by its ability to inhibit production of pigment in Chromobacterium violaceum MTCC 2656 (violacein) and Pseudomonas aeruginosa MTCC 2297 (pyocyanin) or swarming of P. aeruginosa MTCC 2297. Extracts of three plants, Astilbe rivularis, Fragaria nubicola and Osbeckia nepalensis, have shown a dose-dependent inhibition of violacein production with no negative effect on bacterial growth. Inhibition of pyocyanin pigment production and swarming motility in P. aeruginosa MTCC 2297 was also shown. Based on the results obtained by gas chromatography-mass spectroscopy (GC-MS) and thin-layer chromatography-direct bioautography (TLC-DB), it was concluded that triterpenes and flavonoid compounds found in the three plant extracts could have QS-I activity. SIGNIFICANCE AND IMPACT OF THE STUDY: A novel alternative prospect to prevent bacterial infections without inhibiting the growth is to apply chemicals that inhibit quorum sensing mechanism of the pathogens. Antiquorum property of 61 medicinal plants was evaluated by the ability of their leaf extract(s) to inhibit production of pigment (violacein in Chromobacterium violaceum MTCC 2656, pyocyanin in Pseudomonas aeruginosa MTCC 2297) or swarming in P. aeruginosa MTCC 2297. The most prospective plants (for the development of quorum sensing inhibitor), showing inhibition of violacein production without affecting bacterial growth, were Astilbe rivularis, Fragaria nubicola and Osbeckia nepalensis.
[Mh] Termos MeSH primário: Chromobacterium/efeitos dos fármacos
Flavonoides/farmacologia
Indóis/metabolismo
Pseudomonas aeruginosa/efeitos dos fármacos
Piocianina/biossíntese
Percepção de Quorum/efeitos dos fármacos
Triterpenos/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/farmacologia
Fragaria/química
Medicina Tradicional
Melastomataceae/química
Extratos Vegetais/farmacologia
Folhas de Planta/química
Plantas Medicinais/química
Estudos Prospectivos
Saxifragaceae/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Flavonoids); 0 (Indoles); 0 (Plant Extracts); 0 (Triterpenes); 9OQM399341 (Pyocyanine); QJH0DSQ3SG (violacein)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE
[do] DOI:10.1111/lam.12748


  5 / 193 MEDLINE  
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[PMID]:28304342
[Au] Autor:Wang J; Jia Z; Zhang Z; Wang Y; Liu X; Wang L; Lin R
[Ad] Endereço:Beijing Key Laboratory for Quality Evaluation of Traditional Chinese Medicine, School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, China. wangjinfengchn@163.com.
[Ti] Título:Analysis of Chemical Constituents of Melastoma dodecandrum Lour. by UPLC-ESI-Q-Exactive Focus-MS/MS.
[So] Source:Molecules;22(3), 2017 Mar 17.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The ethnic drug Melastoma dodecandrum Lour. (MDL) is widely distributed throughout South China, and is the major component of Gong Yan Ping Tablets/Capsules and Zi Di Ning Xue San. Although the pharmacological effects of MDL have been well documented, its chemical profile has not been fully determined. In this study, we have developed a rapid and sensitive UPLC-ESI-Q-Exactive Focus-MS/MS method to characterize the chemical constituents of MDL in the positive and negative ionization modes. A comparison of the chromatographic and spectrometric data obtained using this method with data from databases, the literature and reference standards allowed us to identify or tentatively characterize 109 compounds, including 26 fatty acids, 26 organic acids, 33 flavonoids, six tannins, 10 triterpenoids, two steroids and six other compounds. Notably, 55 of the compounds characterized in this study have never been detected before in this plant. The information obtained in this study therefore enriches our understanding of the chemical composition of MDL and could be used in quality control, pharmacological research and the development of drugs based on MDL. In addition, this study represents the first reported comprehensive analysis of the chemical constituents of MDL.
[Mh] Termos MeSH primário: Melastomataceae/química
Compostos Fitoquímicos/química
Espectrometria de Massas por Ionização por Electrospray
Espectrometria de Massas em Tandem
[Mh] Termos MeSH secundário: Estrutura Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phytochemicals)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170518
[Lr] Data última revisão:
170518
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170318
[St] Status:MEDLINE


  6 / 193 MEDLINE  
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[PMID]:28284659
[Au] Autor:de Almeida CL; Boeing T; Somensi LB; Steimbach VM; da Silva LM; Andrade SF; Delle Monache F; Cechinel-Filho V; de Souza P
[Ad] Endereço:Programa de Pós-Graduação em Ciências Farmacêuticas, Núcleo de Investigações Químico-Farmacêuticas (NIQFAR), Universidade do Vale do Itajaí (UNIVALI), 88302-901 Itajaí, SC, Brazil.
[Ti] Título:Diuretic, natriuretic and potassium-sparing effect of nothofagin isolated from Leandra dasytricha (A. Gray) Cogn. leaves in normotensive and hypertensive rats.
[So] Source:Chem Biol Interact;268:103-110, 2017 Apr 25.
[Is] ISSN:1872-7786
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Active constituents from natural origin have long been used for the treatment of patients suffering from cardiovascular and renal diseases. This study therefore aimed to investigate the diuretic and natriuretic properties of nothofagin, a dihydrochalcone isolated from Leandra dasytricha (A. Gray) Cogn. leaves in normotensive and hypertensive rats. Male Wistar normotensive rats were orally treated with vehicle (1 ml/kg); hydrochlorothiazide (HCTZ; 25 mg/kg); ethyl acetate fraction from L. dasytricha (EALD; 3-30 mg/kg) and nothofagin (NOT; 0.3-3 mg/kg). Spontaneously hypertensive rats (SHR) received NOT (1 mg/kg), HCTZ (25 mg/kg) or vehicle. The cumulative diuretic index, urinary electrolytes excretion (Na and K ), pH, density and conductivity were measured at the end of the experiment (after 8 h). A7r5 and L929 cell lines were used to measure cell viability after exposure to NOT. Nitric oxide generation was quantified in A7r5 cell supernatant, and DPPH assay was used for evaluating the antioxidant properties of NOT. The urinary volume of normotensive rats were increased after the treatment with EALD, without any changes in Na or K excretion. NOT was able to induce diuresis and natriuresis, but not kaliuresis, in both normotensive and hypertensive rats. The reduction in prostanoids generation through cyclooxygenase inhibition, as well as the muscarinic receptor antagonism, fully avoided NOT-induced increases in diuretic index. NOT, which did not interfere with L929 or A7r5 cell viability, was able to stimulate nitric oxide generation in A7r5 cell, besides showing an antioxidant effect in scavenging the free-radical DPPH. Taken together, our study shows, for the first time, the diuretic, natriuretic and potassium-sparing effect of nothofagin in rats, which was associated with prostanoids generation, muscarinic receptor activation and antioxidant properties.
[Mh] Termos MeSH primário: Antioxidantes/uso terapêutico
Chalconas/uso terapêutico
Diurético Poupador de Potássio/uso terapêutico
Hipertensão/tratamento farmacológico
Melastomataceae/química
Natriuréticos/uso terapêutico
[Mh] Termos MeSH secundário: Animais
Antioxidantes/isolamento & purificação
Antioxidantes/farmacologia
Linhagem Celular
Chalconas/isolamento & purificação
Chalconas/farmacologia
Diurético Poupador de Potássio/isolamento & purificação
Diurético Poupador de Potássio/farmacologia
Hidroclorotiazida/farmacologia
Hidroclorotiazida/uso terapêutico
Hipertensão/metabolismo
Hipopotassemia/prevenção & controle
Masculino
Camundongos
Natriuréticos/isolamento & purificação
Natriuréticos/farmacologia
Óxido Nítrico/metabolismo
Nitritos/metabolismo
Potássio/urina
Prostaglandinas/biossíntese
Ratos Wistar
Receptores Muscarínicos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Chalcones); 0 (Diuretics, Potassium Sparing); 0 (Natriuretic Agents); 0 (Nitrites); 0 (Prostaglandins); 0 (Receptors, Muscarinic); 0 (nothofagin); 0J48LPH2TH (Hydrochlorothiazide); 31C4KY9ESH (Nitric Oxide); RWP5GA015D (Potassium)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170425
[Lr] Data última revisão:
170425
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170313
[St] Status:MEDLINE


  7 / 193 MEDLINE  
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[PMID]:28218717
[Au] Autor:Zhou T; Xu DP; Lin SJ; Li Y; Zheng J; Zhou Y; Zhang JJ; Li HB
[Ad] Endereço:Guangdong Provincial Key Laboratory of Food, Nutrition and Health, School of Public Health, Sun Yat-Sen University, Guangzhou 510080, China. zhout43@mail2.sysu.edu.cn.
[Ti] Título:Ultrasound-Assisted Extraction and Identification of Natural Antioxidants from the Fruit of Melastoma sanguineum Sims.
[So] Source:Molecules;22(2), 2017 Feb 18.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The fruit of Sims is an edible and sweet wild fruit. In our previous study, the fruit was found to have a strong antioxidant property. In this study, an ultrasound-assisted extraction (UAE) method was developed to extract natural antioxidants from the fruit of Sims, and a response surface methodology was used to optimize the conditions of UAE to maximize the extraction efficiency. The influence of five independent extraction parameters (ethanol concentration, solvent/material ratio, extracting time, temperature, and ultrasound power) on the extraction efficiency were investigated using a single factor experiment, and then a central composite rotatable design was used to investigate the interaction of three key parameters. The results showed that the optimal extraction conditions were 42.98% ethanol, 28.29 mL/g solvent/material ratio, 34.29 min extracting time, 60 °C temperature, and 600 W ultrasound power. Under these conditions, the Trolox equivalent antioxidant capacity (TEAC) value of the extracts was 1074.61 ± 32.56 µmol Trolox/g dry weight (DW). Compared with conventional maceration (723.27 ± 11.61 µmol Trolox/g DW) and Soxhlet extraction methods (518.37 ± 23.23 µmol Trolox/g DW), the UAE method improved the extraction efficiency, in a shorter period of time. In addition, epicatechin gallate, epicatechin, rutin, epigallocatechin, protocatechuic acid, chlorogenic acid, and quercetin, were identified and quantified in the fruit extracts of Sims by UPLC-MS/MS.
[Mh] Termos MeSH primário: Antioxidantes/química
Antioxidantes/farmacologia
Fracionamento Químico
Frutas/química
Melastomataceae/química
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Ondas Ultrassônicas
[Mh] Termos MeSH secundário: Fenóis
Solventes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Phenols); 0 (Plant Extracts); 0 (Solvents)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170221
[St] Status:MEDLINE


  8 / 193 MEDLINE  
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[PMID]:28168011
[Au] Autor:Ismail Suhaimy NW; Noor Azmi AK; Mohtarrudin N; Omar MH; Tohid SF; Cheema MS; Teh LK; Salleh MZ; Zakaria ZA
[Ad] Endereço:Department of Biomedical Science, Faculty of Medicine and Health Science, Universiti Putra Malaysia, 43400 Serdang, Selangor, Malaysia.
[Ti] Título:Semipurified Ethyl Acetate Partition of Methanolic Extract of Leaves Exerts Gastroprotective Activity Partly via Its Antioxidant-Antisecretory-Anti-Inflammatory Action and Synergistic Action of Several Flavonoid-Based Compounds.
[So] Source:Oxid Med Cell Longev;2017:6542631, 2017.
[Is] ISSN:1942-0994
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Recent study has demonstrated the gastroprotective activity of crude methanolic extract of leaves. The present study evaluated the gastroprotective potential of semipurified extracts (partitions): petroleum ether, ethyl acetate (EAMM), and aqueous obtained from the methanolic extract followed by the elucidation of the gastroprotective mechanisms of the most effective partition. Using the ethanol-induced gastric ulcer assay, all partitions exerted significant gastroprotection, with EAMM being the most effective partition. EAMM significantly (i) reduced the volume and acidity (free and total) while increasing the pH of gastric juice and enhanced the gastric wall mucus secretion when assessed using the pylorus ligation assay, (ii) increased the enzymatic and nonenzymatic antioxidant activity of the stomach tissue, (iii) lost its gastroprotective activity following pretreatment with N-omega-nitro-L-arginine methyl ester (L-NAME; NO blocker) or carbenoxolone (CBXN; NP-SH blocker), (iv) exerted antioxidant activity against various in vitro oxidation assays, and (v) showed moderate in vitro anti-inflammatory activity via the LOX-modulated pathway. In conclusion, EAMM exerts a remarkable NO/NP-SH-dependent gastroprotective effect that is attributed to its antisecretory and antioxidant activities, ability to stimulate the gastric mucus production and endogenous antioxidant system, and synergistic action of several gastroprotective-induced flavonoids.
[Mh] Termos MeSH primário: Flavonoides/farmacologia
Melastomataceae/química
Extratos Vegetais/química
Úlcera Gástrica/tratamento farmacológico
[Mh] Termos MeSH secundário: Acetatos
Animais
Antioxidantes
Folhas de Planta/química
Úlcera Gástrica/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Antioxidants); 0 (Flavonoids); 0 (Plant Extracts); 76845O8NMZ (ethyl acetate)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170320
[Lr] Data última revisão:
170320
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE
[do] DOI:10.1155/2017/6542631


  9 / 193 MEDLINE  
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[PMID]:28152388
[Au] Autor:Srinivasan R; Natarajan D; Shivakumar MS
[Ad] Endereço:Department of Biotechnology, K.S. Rangasamy College of Arts and Science (Autonomous), Tiruchengode, 637 215 Namakkal, Tamil Nadu, India.
[Ti] Título:Spectral characterization and antibacterial activity of an isolated compound from Memecylon edule leaves.
[So] Source:J Photochem Photobiol B;168:20-24, 2017 Mar.
[Is] ISSN:1873-2682
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Memecylon edule Roxb. (Melastamataceae family) is a small evergreen tree reported as having ethnobotanical and pharmacological properties. The present study was aimed to investigate the spectral characterization and antibacterial activity of isolated pure compound (3ß-hydroxyurs-12-en-28-oic acid (ursolic acid)) from Memecylon edule leaves by performing bioassay guided isolation method. The structure derivation of isolated compound was done by different spectral studies like UV, FT-IR, LC-MS, CHNS analysis, 1D ( H, C and DEPT-135) and 2D-NMR (HSQC and HMBC), respectively. About 99.29% purity of the compound was found in LC analysis. H NMR spectrum results of compound shown 48 protons appear at different shielded region and most of the protons were present in aliphatic region. Whereas, C NMR spectral data resulted seven methyl carbons (CH3), nine methylene carbons (CH2), seven methine carbons (CH) and six non-hydrogenated carbons (C) which are characteristic of pentacyclic triterpene. The isolated pure compound was tested for its antibacterial properties against targeted human pathogens by performing agar well diffusion, MIC and MBC assays and the result exhibits better growth inhibitory effects against S. epidermidis and S. pneumoniae, with the MIC values of 1.56 and 3.15µg/ml. The outcome of this study suggests that the bioactive compound is used for development of plant based drugs in pharmaceutical industry for combating microbial mediated diseases.
[Mh] Termos MeSH primário: Antibacterianos/isolamento & purificação
Extratos Vegetais/química
Plantas Medicinais
Análise Espectral/métodos
Triterpenos/isolamento & purificação
[Mh] Termos MeSH secundário: Antibacterianos/química
Antibacterianos/farmacologia
Seres Humanos
Melastomataceae
Testes de Sensibilidade Microbiana
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/farmacologia
Folhas de Planta/química
Staphylococcus/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Plant Extracts); 0 (Triterpenes); P3M2575F3F (ursolic acid)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE


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[PMID]:28117630
[Au] Autor:Ferreira BG; Falcioni R; Guedes LM; Avritzer SC; Antunes WC; Souza LA; Isaias RM
[Ad] Endereço:Departamento de Botânica/Pós-Graduação em Biologia Vegetal, Instituto de Ciências Biológicas, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil (BGF, SCA, RMSI).
[Ti] Título:Preventing False Negatives for Histochemical Detection of Phenolics and Lignins in PEG-Embedded Plant Tissues.
[So] Source:J Histochem Cytochem;65(2):105-116, 2017 02.
[Is] ISSN:1551-5044
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Polyethylene glycol (PEG) is a low-cost and advantageous embedding medium, which maintains the majority of cell contents unaltered during the embedding process. Some hard or complex plant materials are better embedded in PEG than in other usual embedding media. However, the histochemical tests for phenolics and lignins in PEG-embedded plant tissues commonly result in false negatives. We hypothesize that these false negatives should be prevented by the use of distinct fixatives, which should avoid the bonds between PEG and phenols. Novel protocols for phenolics and flavanols detection are efficiently tested, with fixation of the samples in ferrous sulfate and formalin or in caffeine and sodium benzoate, respectively. The differentiation of lignin types is possible in safranin-stained sections observed under fluorescence. The Maule's test faultlessly distinguishes syringyl-rich from guaiacyl- and hydroxyphenyl-rich lignins in PEG-embedded material under light microscopy. Current hypothesis is corroborated, that is, the adequate fixation solves the false-negative results, and the new proposed protocols fill up some gaps on the detection of phenolics and lignins.
[Mh] Termos MeSH primário: Lignina/análise
Fenóis/química
Plantas/química
Plantas/ultraestrutura
Polietilenoglicóis/química
Inclusão do Tecido/métodos
[Mh] Termos MeSH secundário: Anacardiaceae/química
Anacardiaceae/ultraestrutura
Fixadores/química
Flavonóis/análise
Melastomataceae/química
Melastomataceae/ultraestrutura
Microscopia de Fluorescência/métodos
Polifenóis/análise
Coloração e Rotulagem/métodos
Taninos/análise
Fixação de Tecidos/métodos
Tabaco/química
Tabaco/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fixatives); 0 (Flavonols); 0 (Phenols); 0 (Polyphenols); 0 (Tannins); 30IQX730WE (Polyethylene Glycols); 9005-53-2 (Lignin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE
[do] DOI:10.1369/0022155416677035



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