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Pesquisa : B01.650.940.800.575.912.250.725 [Categoria DeCS]
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  1 / 105 MEDLINE  
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[PMID]:28714672
[Au] Autor:Malhotra P; Jethva PN; Udgaonkar JB
[Ad] Endereço:National Centre for Biological Sciences , Tata Institute of Fundamental Research, Bengaluru 560065, India.
[Ti] Título:Chemical Denaturants Smoothen Ruggedness on the Free Energy Landscape of Protein Folding.
[So] Source:Biochemistry;56(31):4053-4063, 2017 Aug 08.
[Is] ISSN:1520-4995
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To characterize experimentally the ruggedness of the free energy landscape of protein folding is challenging, because the distributed small free energy barriers are usually dominated by one, or a few, large activation free energy barriers. This study delineates changes in the roughness of the free energy landscape by making use of the observation that a decrease in ruggedness is accompanied invariably by an increase in folding cooperativity. Hydrogen exchange (HX) coupled to mass spectrometry was used to detect transient sampling of local energy minima and the global unfolded state on the free energy landscape of the small protein single-chain monellin. Under native conditions, local noncooperative openings result in interconversions between Boltzmann-distributed intermediate states, populated on an extremely rugged "uphill" energy landscape. The cooperativity of these interconversions was increased by selectively destabilizing the native state via mutations, and further by the addition of a chemical denaturant. The perturbation of stability alone resulted in seven backbone amide sites exchanging cooperatively. The size of the cooperatively exchanging and/or unfolding unit did not depend on the extent of protein destabilization. Only upon the addition of a denaturant to a destabilized mutant variant did seven additional backbone amide sites exchange cooperatively. Segmentwise analysis of the HX kinetics of the mutant variants further confirmed that the observed increase in cooperativity was due to the smoothing of the ruggedness of the free energy landscape of folding of the protein by the chemical denaturant.
[Mh] Termos MeSH primário: Guanidina/química
Indicadores e Reagentes/química
Menispermaceae/metabolismo
Modelos Moleculares
Proteínas de Plantas/química
[Mh] Termos MeSH secundário: Substituição de Aminoácidos
Medição da Troca de Deutério
Transferência de Energia/efeitos dos fármacos
Cinética
Mutagênese Sítio-Dirigida
Mutação
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Conformação Proteica/efeitos dos fármacos
Desnaturação Proteica/efeitos dos fármacos
Dobramento de Proteína/efeitos dos fármacos
Estabilidade Proteica/efeitos dos fármacos
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indicators and Reagents); 0 (Plant Proteins); 0 (monellin protein, Dioscoreophyllum cumminsii); JU58VJ6Y3B (Guanidine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170718
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biochem.7b00367


  2 / 105 MEDLINE  
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[PMID]:28301470
[Au] Autor:Iloh AC; Schmidt M; Muellner-Riehl AN; Ogundipe OT; Paule J
[Ad] Endereço:Senckenberg Biodiversity and Climate Research Centre (BiK-F), Senckenberganlage 25, Frankfurt am Main, Germany.
[Ti] Título:Pleistocene refugia and genetic diversity patterns in West Africa: Insights from the liana Chasmanthera dependens (Menispermaceae).
[So] Source:PLoS One;12(3):e0170511, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Processes shaping the African Guineo-Congolian rain forest, especially in the West African part, are not well understood. Recent molecular studies, based mainly on forest tree species, confirmed the previously proposed division of the western African Guineo-Congolian rain forest into Upper Guinea (UG) and Lower Guinea (LG) separated by the Dahomey Gap (DG). Here we studied nine populations in the area of the DG and the borders of LG and UG of the widespread liana species, Chasmanthera dependens (Menispermaceae) by amplified fragment length polymorphism (AFLP), a chloroplast DNA sequence marker, and modelled the distribution based on current as well as paleoclimatic data (Holocene Climate Optimum, ca. 6 kyr BP and Last Glacial Maximum, ca. 22 kyr BP). Current population genetic structure and geographical pattern of cpDNA was related to present as well as historical modelled distributions. Results from this study show that past historical factors played an important role in shaping the distribution of C. dependens across West Africa. The Cameroon Volcanic Line seems to represent a barrier for gene flow in the present as well as in the past. Distribution modelling proposed refugia in the Dahomey Gap, supported also by higher genetic diversity. This is in contrast with the phylogeographic patterns observed in several rainforest tree species and could be explained by either diverging or more relaxed ecological requirements of this liana species.
[Mh] Termos MeSH primário: Variação Genética
Menispermaceae/genética
[Mh] Termos MeSH secundário: África Ocidental
Análise do Polimorfismo de Comprimento de Fragmentos Amplificados
Animais
DNA de Cloroplastos/genética
Haplótipos
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Chloroplast)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0170511


  3 / 105 MEDLINE  
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[PMID]:28049039
[Au] Autor:Wang W; Ortiz RDC; Jacques FMB; Chung SW; Liu Y; Xiang XG; Chen ZD
[Ad] Endereço:State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China; University of Chinese Academy of Sciences, Beijing 100049, China. Electronic address: wangwei1127@ibcas.ac.cn.
[Ti] Título:New insights into the phylogeny of Burasaieae (Menispermaceae) with the recognition of a new genus and emphasis on the southern Taiwanese and mainland Chinese disjunction.
[So] Source:Mol Phylogenet Evol;109:11-20, 2017 Apr.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Taiwan is a continental island lying at the boundary between the Eurasian and the Philippine tectonic plates and possesses high biodiversity. Southern Taiwan, viz. Hengchun Peninsula, is notably floristically different from northern Taiwan. The floristic origin and relationships of the Hengchun Peninsula have been rarely investigated in a phylogenetic context. In this study, data from six plastid and nuclear sequences were used to reconstruct phylogenetic relationships within Burasaieae (Menispermaceae), which mainly inhabits tropical rainforests. The tree-based comparisons indicate that the position of Tinospora sensu stricto conflicts significantly between the cpDNA and ITS trees. However, alternative hypothesis tests from the ITS data did not reject the result of the cpDNA data, which suggests that tree-based comparisons might sometimes generate an artificial incongruence, especially when markers with high homoplasy are used. Based on the combined cpDNA and ITS data, we present an inter-generic phylogenetic framework for Burasaieae. Sampled species of Tinospora are placed in three different clades, including Tinospora dentata from southern Taiwan and T. sagittata from mainland China in an unresolved position alongside six lineages of Burasaieae. By integrating lines of evidence from molecular phylogeny, divergence times, and morphology, we recognize the three Tinospora clades as three different genera, including Tinospora sensu stricto, a new genus (Paratinospora) for T. dentata and T. sagittata, and Hyalosepalum resurrected. Tinospora dentata, now endemic to the Hengchun Peninsula, originated from the Late Eocene (ca. 39Ma), greatly predating the formation of Taiwan. Our study suggests that the flora of the Hengchun Peninsula contains some ancient components that might have migrated from mainland China.
[Mh] Termos MeSH primário: Menispermaceae/classificação
[Mh] Termos MeSH secundário: China
DNA de Plantas/genética
Genes de Plantas
Especiação Genética
Menispermaceae/genética
Filogenia
Filogeografia
Proteínas de Plantas/genética
Plastídeos/genética
Análise de Sequência de DNA
Taiwan
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); 0 (Plant Proteins)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170924
[Lr] Data última revisão:
170924
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170104
[St] Status:MEDLINE


  4 / 105 MEDLINE  
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[PMID]:28033583
[Au] Autor:T VK; M V; M G; Das P; Roy P; Banerjee A; Dutta Gupta S
[Ad] Endereço:Department of Pharmacology, Gokaraju Rangaraju College of Pharmacy, Osmania University, Hyderabad, Telangana State, India.
[Ti] Título:Evaluation of antibacterial, antioxidant and nootropic activities of Tiliacora racemosa Colebr. leaves: In vitro and in vivo approach.
[So] Source:Biomed Pharmacother;86:662-668, 2017 Feb.
[Is] ISSN:1950-6007
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:The antibacterial and antioxidant potential of Tiliacora racemosa leaf extracts in various solvents (methanolic, hexane, chloroform and ethyl acetate) was determined. Additionally, the presence of bisbenzylisoquinoline alkaloids in the plant prompted us to evaluate the nootropic activity of the methanolic extract in mice. Further, we seek to verify the nootropic effect by examining the anticholinesterase inhibition potential of the methanolic extract. The leaf extracts in various solvents were evaluated for their antibacterial and antioxidant activity by agar diffusion technique and α, α-diphenyl-ß-picrylhydrazyl (DPPH) free radical scavenging method, respectively. The ex vivo acetylcholine esterase inhibitory activity of the methanolic extract was carried out by Ellman's method in male Wistar rats. The nootropic capacity of the methanolic extract was examined in Swiss albino mice by utilizing the diazepam induced acute amnesic model. The chloroform/n-hexane and ethyl acetate fraction showed promising antioxidant and antibacterial (Gram positive and Gram negative bacteria) property, respectively. The methanolic extract was able to diminish the amnesic effect induced by diazepam (1mg/kg i.p.) in mice. The extract also showed significant acetyl cholinesterase inhibition in rats. The findings prove that the memory enhancing capability is due to increased acetyl choline level at the nerve endings. The strong antioxidant nature and potential nootropic activity shown by the extract suggests its future usage in the treatment of neurodegenerative disorders such as dementia and Alzheimer.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Antioxidantes/farmacologia
Menispermaceae/química
Nootrópicos/farmacologia
Folhas de Planta/química
[Mh] Termos MeSH secundário: Alcaloides/química
Alcaloides/farmacologia
Animais
Inibidores da Colinesterase/química
Inibidores da Colinesterase/farmacologia
Depuradores de Radicais Livres/química
Depuradores de Radicais Livres/farmacologia
Hexanos/química
Masculino
Camundongos
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Anti-Bacterial Agents); 0 (Antioxidants); 0 (Cholinesterase Inhibitors); 0 (Free Radical Scavengers); 0 (Hexanes); 0 (Nootropic Agents); 0 (Plant Extracts); 2DDG612ED8 (n-hexane)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170208
[Lr] Data última revisão:
170208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161230
[St] Status:MEDLINE


  5 / 105 MEDLINE  
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[PMID]:27976592
[Au] Autor:Dary C; Bun SS; Herbette G; Mabrouki F; Bun H; Kim S; Jabbour F; Hul S; Baghdikian B; Ollivier E
[Ad] Endereço:a Laboratory of Pharmacognosy and Ethnopharmacology, Faculty of Pharmacy , Aix Marseille Univ, UMR-MD3 , Marseille , France.
[Ti] Título:Chemical profiling of the tuber of Stephania cambodica Gagnep. (Menispermaceae) and analytical control by UHPLC-DAD.
[So] Source:Nat Prod Res;31(7):802-809, 2017 Apr.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new aporphine glycoside (1), named 'angkorwatine', and eight known alkaloids: oblongine (2), stepharine (3), asimilobine-ß-d-glucopyranoside (4), isocorydine (5), tetrahydropalmatine (THP) (6), jatrorrhizine (7), palmatine (PAL) (8), and roemerine (ROE) (9) were simultaneously isolated from the tuber of Stephania cambodica. The development and validation of UHPLC-DAD method was carried out for the quantification of marker compounds (PAL, ROE, THP) of S. cambodica. In addition to good selectivity and linearity (r > 0.997), trueness, precision, and accuracy of the method did not exceed the acceptance limit of ±10% for ROE, THP and ±20% for PAL. Consequently, this method is able to provide accurate results between 1.39-4.18 µg/mL, 2.01-30.72 µg/mL, and 4.29-64.42 µg/mL for PAL, ROE, and THP, respectively. This study shows that the validated UHPLC method is a rapid, innovative and effective analytical approach to control quality of tubers of S. cambodica and to regulate the usage of this plant in traditional medicine.
[Mh] Termos MeSH primário: Menispermaceae/química
Tubérculos/química
Stephania/química
[Mh] Termos MeSH secundário: Alcaloides/química
Aporfinas
Alcaloides de Berberina
Cromatografia Líquida de Alta Pressão
Isoquinolinas
Espectroscopia de Ressonância Magnética
Reprodutibilidade dos Testes
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Aporphines); 0 (Berberine Alkaloids); 0 (Isoquinolines); 13NS2KTD6H (aporphine); 2810-21-1 (stepharine); 3B5E87JEOX (isocorydine); 3X69CO5I79 (tetrahydropalmatine); 548-08-3 (roemerine); 60008-01-7 (oblongine); 6871-21-2 (asimilobine); G50C034217 (palmatine)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161216
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2016.1247077


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[PMID]:27373319
[Au] Autor:Samita F; Ochieng CO; Owuor PO; Manguro LO; Midiwo JO
[Ad] Endereço:a Department of Chemistry, School of Physical and Biological Sciences , Maseno University , Maseno , Kenya.
[Ti] Título:Isolation of a new ß-carboline alkaloid from aerial parts of Triclisia sacleuxii and its antibacterial and cytotoxicity effects.
[So] Source:Nat Prod Res;31(5):529-536, 2017 Mar.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new ß-carboline alkaloid named sacleuximine A (1) together with known compounds palmatine (2), isotetrandrine (3), trans-N-feruloyltyramine (4), trans-N-caffeoyltyramine (5), yangambin (6), syringaresinol (7), sesamin (8), (+) epi-quercitol (9), 4-hydroxybenzaldehyde (10), ß-sitosterol (11), quercetin 3-O-rutinoside (12) and myricetin 3-O-ß-glucose (1→6) α-rhamnoside (13) have been isolated from methanol extract of Triclisia sacleuxii aerial parts. Compounds 1-10 were evaluated for their cytotoxicity against human adenocarcinoma (HeLa), human hepatocarcinoma (Hep3B) and human breast carcinoma (MCF-7) cells lines and also for antibacterial activities against both Gram-positive and Gram-negative bacteria. The cytotoxicity (IC ) values ranged between 0.15 and 36.7 µM while the minimum inhibitory concentrations were found to be in the range of 3.9 and 125 µM, respectively. This is the first report of antibacterial compounds and the isolation of lignans together with a ß-carboline alkaloid from T. sacleuxii.
[Mh] Termos MeSH primário: Alcaloides/isolamento & purificação
Antibacterianos/farmacologia
Antineoplásicos Fitogênicos/farmacologia
Carbolinas/isolamento & purificação
Menispermaceae/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Alcaloides/química
Alcaloides/farmacologia
Carbolinas/química
Carbolinas/farmacologia
Linhagem Celular Tumoral
Seres Humanos
Testes de Sensibilidade Microbiana
Componentes Aéreos da Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Anti-Bacterial Agents); 0 (Antineoplastic Agents, Phytogenic); 0 (Carbolines); 0 (Plant Extracts)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160705
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2016.1201666


  7 / 105 MEDLINE  
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[PMID]:27568876
[Au] Autor:Ajiboye TO; Aliyu H; Tanimu MA; Muhammad RM; Ibitoye OB
[Ad] Endereço:Antioxidants, Free Radicals, Functional Foods and Toxicology Research Laboratory, Department of Biological Sciences, Al-Hikmah University, Ilorin, Nigeria. Electronic address: ajiboyeyong@yahoo.com.
[Ti] Título:Dioscoreophyllum cumminsii (Stapf) Diels leaves halt high-fructose induced metabolic syndrome: Hyperglycemia, insulin resistance, inflammation and oxidative stress.
[So] Source:J Ethnopharmacol;192:471-479, 2016 Nov 04.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Dioscoreophyllum cumminsii is widely used in the management and treatment of diabetes and obesity in Nigeria. This study evaluates the effect of aqueous leaf extract of D. cumminsii on high-fructose diet-induced metabolic syndrome. METHODS: Seventy male rats were randomized into seven groups. All rats were fed with high-fructose diet for 9 weeks except groups A and C rats, which received control diet. In addition to the diet treatment, groups A and B rats received distilled water for 3 weeks starting from the seventh week of the experimental period. Rats in groups C-F orally received 400, 100, 200 and 400mg/kg body weight of aqueous leaf extract of D. cumminsii respectively, while group G received 300mg/kg bodyweight of metformin for 3 weeks starting from the seventh week. RESULTS: There was significant (p<0.05) reduction in high-fructose diet-mediated increase in body weight, body mass index, abdominal circumference, blood glucose, insulin, leptin and insulin resistance by aqueous leaf extract of D. cumminsii. Conversely, high-fructose diet-mediated decrease in adiponectin was reversed by the extract. Increased levels of cholesterol, triglycerides, low-density lipoprotein cholesterol, very low-density lipoprotein cholesterol, atherogenic index, cardiac index and coronary artery index were significantly lowered by the extract, while high-fructose diet mediated decrease in high-density lipoprotein cholesterol was increased by the extract. Tumour necrosis factor-α, interleukin-6 and interleukin-8 levels increased significantly in high-fructose diet-fed rats, which were significantly reversed by the extract. High-fructose mediated-decrease in superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glucose 6-phosphate dehydrogenase and glutathione reduced were significantly reversed by aqueous leaf extract of D. cumminsii. Conversely, elevated levels of malondialdehyde, conjugated dienes, lipid hydroperoxides, protein carbonyl and fragmented DNA were significantly lowered by the extract. CONCLUSION: Data generated in this study further laid credence to the hypoglycemic activity of aqueous leaf extract of D. cumminsii as evident from the reversal of hyperglycemia, insulin resistance, dyslipidemia, inflammation and oxidative stress in high-fructose diet-induced metabolic syndrome rats.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Antioxidantes/farmacologia
Glicemia/efeitos dos fármacos
Frutose
Hipoglicemiantes/farmacologia
Mediadores da Inflamação/sangue
Resistência à Insulina
Menispermaceae/química
Síndrome Metabólica/prevenção & controle
Estresse Oxidativo/efeitos dos fármacos
Extratos Vegetais/farmacologia
Folhas de Planta/química
[Mh] Termos MeSH secundário: Adiposidade/efeitos dos fármacos
Animais
Anti-Inflamatórios/isolamento & purificação
Antioxidantes/isolamento & purificação
Biomarcadores/sangue
Glicemia/metabolismo
Fragmentação do DNA/efeitos dos fármacos
Modelos Animais de Doenças
Relação Dose-Resposta a Droga
Dislipidemias/sangue
Dislipidemias/induzido quimicamente
Dislipidemias/prevenção & controle
Hipoglicemiantes/isolamento & purificação
Hipolipemiantes/isolamento & purificação
Hipolipemiantes/farmacologia
Insulina/sangue
Peroxidação de Lipídeos/efeitos dos fármacos
Lipídeos/sangue
Fígado/efeitos dos fármacos
Fígado/metabolismo
Fígado/patologia
Masculino
Síndrome Metabólica/sangue
Síndrome Metabólica/induzido quimicamente
Síndrome Metabólica/patologia
Fitoterapia
Extratos Vegetais/isolamento & purificação
Plantas Medicinais
Carbonilação Proteica/efeitos dos fármacos
Ratos Wistar
Solventes/química
Fatores de Tempo
Água/química
Ganho de Peso/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Biomarkers); 0 (Blood Glucose); 0 (Hypoglycemic Agents); 0 (Hypolipidemic Agents); 0 (Inflammation Mediators); 0 (Insulin); 0 (Lipids); 0 (Plant Extracts); 0 (Solvents); 059QF0KO0R (Water); 30237-26-4 (Fructose)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160830
[St] Status:MEDLINE


  8 / 105 MEDLINE  
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[PMID]:27270992
[Au] Autor:Uche FI; Drijfhout FP; McCullagh J; Richardson A; Li WW
[Ad] Endereço:Institute for Science and Technology in Medicine, Keele University, Staffordshire, UK.
[Ti] Título:Cytotoxicity Effects and Apoptosis Induction by Bisbenzylisoquinoline Alkaloids from Triclisia subcordata.
[So] Source:Phytother Res;30(9):1533-9, 2016 Sep.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Triclisia subcordata Oliv (Menispermeaceae) is a medicinal plant traditionally used for the treatment of various diseases in West Africa. The ethanol extract of T. subcordata and its fractions were screened for in vitro anti-ovarian cancer activities using the Sulforhodamine B assay. The crude alkaloids showed the strongest activity in cell growth assays on Ovcar-8 and A2780 cell lines (IC50 < 2.4 µg/mL). A bisbenzylisoquinoline alkaloid-cycleanine was isolated using HPLC and identified by mass spectrometry and nuclear magnetic resonance analyses. The IC50 values of cycleanine and tetrandrine (an alkaloid previously reported from this plant) ranged from 7 to 14 µM on Ovcar-8, A2780, Ovcar-4, and Igrov-1 ovarian cancer cell lines. The IC50 of cycleanine on human normal ovarian surface epithelial cells was 35 ± 1 µM, hinting at modest selectivity toward cancer cells. Both cycleanine and tetrandrine caused apoptosis as shown by activation of caspases 3/7 and cleavage of poly(ADP-ribose) polymerase to form poly(ADP-ribose) polymerase-1 by using western blot analysis. Flow cytometry analyses showed that the percentages of apoptotic cells and cells in subG1 phase increased after exposure of cycleanine and tetrandrine to Ovcar-8 cells for 48 h compared with control. Cycleanine, like its isomer tetrandrine isolated from T. subcordata, could be a potential new anti-ovarian cancer agent acting through the apoptosis pathway. Copyright © 2016 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Benzilisoquinolinas/farmacologia
Menispermaceae/química
Neoplasias Ovarianas/tratamento farmacológico
Plantas Medicinais/química
[Mh] Termos MeSH secundário: Apoptose
Proliferação Celular/efeitos dos fármacos
Feminino
Seres Humanos
Neoplasias Ovarianas/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Benzylisoquinolines)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160609
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5660


  9 / 105 MEDLINE  
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[PMID]:26452875
[Au] Autor:Wei J; Fang L; Liang X; Su D; Guo X
[Ad] Endereço:Department of Pharmaceutical Analysis, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang 110016, China.
[Ti] Título:A sensitive and selective UPLC-MS/MS method for simultaneous determination of 10 alkaloids from Rhizoma Menispermi in rat plasma and its application to a pharmacokinetic study.
[So] Source:Talanta;144:662-70, 2015 Nov 01.
[Is] ISSN:1873-3573
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A sensitive and selective liquid chromatography-tandem mass spectrometry method has been developed and validated for simultaneous quantitation of 10 alkaloids (dauricine, daurisoline, N-desmethyldauricine, dauricicoline, dauriporphinoline, bianfugecine, dauricoside, stepholidine, acutumine and acutumidine) from Rhizoma Menispermi in rat plasma. After addition of internal standard (verapamil), plasma samples were pretreated by a single-step protein precipitation with acetonitrile. Chromatographic separation was performed on a Waters BEH C18 column with gradient elution using a mobile phase composed of acetonitrile and water (containing 0.1% formic acid) at a flow rate of 0.3 mL/min. The analytes were detected without interference in the multiple reaction monitoring (MRM) mode with positive electrospray ionization. The validated method exhibited good linearity over a wide concentration range (r≥0.9914), and the lower limits of quantification were 0.01-5.0 ng/mL for all the analytes. The intra-day and inter-day precisions (RSD) at three different levels were both less than 13.4% and the accuracies (RE) ranged from -12.8% to 13.5%. The mean extraction recoveries of analytes and IS from rat plasma were all more than 77%. The validated method was successfully applied to a comparative pharmacokinetic study of 10 alkaloids in rat plasma after oral administration of Rhizoma Menispermi extract.
[Mh] Termos MeSH primário: Alcaloides/sangue
Menispermaceae
Rizoma/química
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida
Masculino
Extratos Vegetais/sangue
Extratos Vegetais/química
Extratos Vegetais/farmacocinética
Ratos Wistar
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Plant Extracts)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:151010
[Lr] Data última revisão:
151010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151011
[St] Status:MEDLINE


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[PMID]:26384972
[Au] Autor:Hagel JM; Morris JS; Lee EJ; Desgagné-Penix I; Bross CD; Chang L; Chen X; Farrow SC; Zhang Y; Soh J; Sensen CW; Facchini PJ
[Ad] Endereço:Department of Biological Sciences, University of Calgary, Calgary, AB, T2N 1N4, Canada. hagelj@ucalgary.ca.
[Ti] Título:Transcriptome analysis of 20 taxonomically related benzylisoquinoline alkaloid-producing plants.
[So] Source:BMC Plant Biol;15:227, 2015 Sep 18.
[Is] ISSN:1471-2229
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Benzylisoquinoline alkaloids (BIAs) represent a diverse class of plant specialized metabolites sharing a common biosynthetic origin beginning with tyrosine. Many BIAs have potent pharmacological activities, and plants accumulating them boast long histories of use in traditional medicine and cultural practices. The decades-long focus on a select number of plant species as model systems has allowed near or full elucidation of major BIA pathways, including those of morphine, sanguinarine and berberine. However, this focus has created a dearth of knowledge surrounding non-model species, which also are known to accumulate a wide-range of BIAs but whose biosynthesis is thus far entirely unexplored. Further, these non-model species represent a rich source of catalyst diversity valuable to plant biochemists and emerging synthetic biology efforts. RESULTS: In order to access the genetic diversity of non-model plants accumulating BIAs, we selected 20 species representing 4 families within the Ranunculales. RNA extracted from each species was processed for analysis by both 1) Roche GS-FLX Titanium and 2) Illumina GA/HiSeq platforms, generating a total of 40 deep-sequencing transcriptome libraries. De novo assembly, annotation and subsequent full-length coding sequence (CDS) predictions indicated greater success for most species using the Illumina-based platform. Assembled data for each transcriptome were deposited into an established web-based BLAST portal ( www.phytometasyn.ca) to allow public access. Homology-based mining of libraries using BIA-biosynthetic enzymes as queries yielded ~850 gene candidates potentially involved in alkaloid biosynthesis. Expression analysis of these candidates was performed using inter-library FPKM normalization methods. These expression data provide a basis for the rational selection of gene candidates, and suggest possible metabolic bottlenecks within BIA metabolism. Phylogenetic analysis was performed for each of 15 different enzyme/protein groupings, highlighting many novel genes with potential involvement in the formation of one or more alkaloid types, including morphinan, aporphine, and phthalideisoquinoline alkaloids. Transcriptome resources were used to design and execute a case study of candidate N-methyltransferases (NMTs) from Glaucium flavum, which revealed predicted and novel enzyme activities. CONCLUSIONS: This study establishes an essential resource for the isolation and discovery of 1) functional homologues and 2) entirely novel catalysts within BIA metabolism. Functional analysis of G. flavum NMTs demonstrated the utility of this resource and underscored the importance of empirical determination of proposed enzymatic function. Publically accessible, fully annotated, BLAST-accessible transcriptomes were not previously available for most species included in this report, despite the rich repertoire of bioactive alkaloids found in these plants and their importance to traditional medicine. The results presented herein provide essential sequence information and inform experimental design for the continued elucidation of BIA metabolism.
[Mh] Termos MeSH primário: Alcaloides/metabolismo
Benzilisoquinolinas/metabolismo
Magnoliopsida/genética
Proteínas de Plantas/genética
Transcriptoma
[Mh] Termos MeSH secundário: Berberidaceae/genética
Berberidaceae/metabolismo
Sequenciamento de Nucleotídeos em Larga Escala
Magnoliopsida/metabolismo
Menispermaceae/genética
Menispermaceae/metabolismo
Dados de Sequência Molecular
Papaveraceae/genética
Papaveraceae/metabolismo
Proteínas de Plantas/metabolismo
Ranunculaceae/genética
Ranunculaceae/metabolismo
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Benzylisoquinolines); 0 (Plant Proteins)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150920
[St] Status:MEDLINE
[do] DOI:10.1186/s12870-015-0596-0



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