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Pesquisa : B01.650.940.800.575.912.250.725.500 [Categoria DeCS]
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[PMID]:27435376
[Au] Autor:Su Q; He J; Wang Z; Lv L; Suo Y; Wang J; Zheng Z; Huo C; Li J
[Ad] Endereço:Key Laboratory of Resource Biology and Biotechnology in Western China (Northwest University), Ministry of Education, Xi'an 710069, China; Biomedicine Key Laboratory of Shaanxi Province, College of Life Science, Northwest University, Xi'an 710069, China.
[Ti] Título:Intestinal anti-inflammatory effect of the rhizome extracts of Menispermum dauricum DC. on trinitrobenzene sulfonic acid induced ulcerative colitis in mice.
[So] Source:J Ethnopharmacol;193:12-20, 2016 Dec 04.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Menispermum dauricum DC., commonly known as "Bei Dou Gen" (BDG) in China, has been used extensively in folk medicine to treat inflammatory diseases, especially intestinal inflammations such as enteritis and dysentery, and in pharyngitis, tonsillitis, rheumatism and bronchitis. Although previous studies showed that BDG has anti-inflammatory activities, its effects on ulcerative colitis (UC) have not yet been explored. AIM OF THE STUDY: To investigate the intestinal anti-inflammatory effect of the rhizome extracts of Menispermum dauricum DC. on UC model induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS) in mice. MATERIALS AND METHODS: UC in mice was induced by colonic administration with TNBS. BDG (100, 200 and 400mg/kg/day) and sulfasalazine (500mg/kg/day) were administered orally for 7 consecutive days. The inflammatory degree was assessed by gross appearance, macroscopic and histological analysis, and accumulation of myeloperoxidase (MPO) activity. Pro-inflammatory mediators, including tumor necrosis factor (TNF)-α, interleukin (IL)-1ß and IL-6, were determined by enzyme-linked immunoassay. The expression of cyclooxygenase (COX)-2 was assessed by immunohistochemical analysis. RESULTS: Treatment with different doses of BDG significantly ameliorated macroscopic damage and histological changes, reduced the accumulation of MPO activity, depressed serum and colonic tissue levels of TNF-α, IL-1ß and IL-6 in a dose-dependent manner. In addition, administration of BDG effectively reduced COX-2 overexpression in colon. CONCLUSION: We demonstrated for the first time that BDG possessed marked intestinal anti-inflammatory effect in TNBS induced colitis in mice, which might be related to the reduction of up-regulated productions and expressions of pro-inflammatory mediators, suggesting that it may have beneficial use for the treatment of inflammatory bowel disease.
[Mh] Termos MeSH primário: Colite Ulcerativa/prevenção & controle
Menispermum/química
Extratos Vegetais/farmacologia
Rizoma/química
Ácido Trinitrobenzenossulfônico/toxicidade
[Mh] Termos MeSH secundário: Animais
Peso Corporal/efeitos dos fármacos
Colite Ulcerativa/induzido quimicamente
Colite Ulcerativa/enzimologia
Ciclo-Oxigenase 2/metabolismo
Citocinas/sangue
Citocinas/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos ICR
Peroxidase/metabolismo
Baço/efeitos dos fármacos
Timo/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokines); 0 (Plant Extracts); 8T3HQG2ZC4 (Trinitrobenzenesulfonic Acid); EC 1.11.1.7 (Peroxidase); EC 1.14.99.1 (Cyclooxygenase 2)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170517
[Lr] Data última revisão:
170517
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160721
[St] Status:MEDLINE


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[PMID]:26094998
[Au] Autor:Wei J; Chen J; Liang X; Guo X
[Ad] Endereço:Shenyang Pharmaceutical University, Shenyang, 103 Wenhua Road, Shenyang, 110016, People's Republic of China.
[Ti] Título:Microwave-assisted extraction in combination with HPLC-UV for quantitative analysis of six bioactive oxoisoaporphine alkaloids in Menispermum dauricum DC.
[So] Source:Biomed Chromatogr;30(2):241-8, 2016 Feb.
[Is] ISSN:1099-0801
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel and reliable method based on microwave-assisted extraction (MAE) followed by HPLC-UV was developed and validated for the simultaneous quantification of six pharmacologically important oxoisoaporphine alkaloids in the total plants of Menispermum dauricum DC. The optimal MAE extraction condition was performed at 60°C for 11 min with ethanol-water (70:30, v/v) as the extracting solvent, and the solvent to solid ratio was 20:1. Chromatographic separation was achieved on a reversed-phase YMC C18 column (250 × 4.6 mm, i.d., 5 µm) with a gradient mobile phase consisting of A (1% aqueous formic acid) and B (acetonitrile containing 1% formic acid) at a flow rate of 1.5 mL/min. The detection wavelength was set at 422 nm. Excellent linearity over the investigated concentration ranges was observed with values of r >0.999 for all analytes. The method developed was validated with acceptable sensitivity, intra- and inter-day precision and extraction recoveries. It was successfully applied to the determination of six alkaloids in Menispermum dauricum DC from different sources and different parts of Menispermum dauricum DC. The results obtained indicated that the method is suitable for the quality control of Menispermum dauricum DC.
[Mh] Termos MeSH primário: Alcaloides/análise
Cromatografia Líquida de Alta Pressão/métodos
Menispermum/química
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Alcaloides/química
Limite de Detecção
Modelos Lineares
Extração Líquido-Líquido
Micro-Ondas
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Plant Extracts)
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160114
[Lr] Data última revisão:
160114
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150623
[St] Status:MEDLINE
[do] DOI:10.1002/bmc.3541


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[PMID]:25948242
[Au] Autor:Wei J; Jiang Z; Cui Z; Guo X
[Ad] Endereço:Department of Analytical Chemistry, School of Pharmacy, Shenyang Pharmaceutical University, 103 Wenhua Road, Shenyang, 110016, Liaoning, China.
[Ti] Título:Rapid determination of eight oxoisoaporphine alkaloids in Rhizoma Menispermi by the optimal homogenate extraction followed by UPLC-MS/MS.
[So] Source:Anal Bioanal Chem;407(18):5535-40, 2015 Jul.
[Is] ISSN:1618-2650
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to develop a rapid and reliable homogenate extraction (HGE) and ultra high-performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method for simultaneous analysis of eight bioactive oxoisoaporphine alkaloids (including two new alkaloids) in Rhizoma Menispermi. HGE was optimized by response surface methodology (RSM) to obtain the maximum extraction efficiency of eight alkaloids. Separation was achieved on a Waters ACQUITY UPLC® BEH C18 column (50 × 2.1 mm(2), 1.7 µm) using gradient elution with a mobile phase consisting of acetonitrile and 0.2% formic acid in water. Quantification was performed with multiple reaction monitoring (MRM) mode using positive ESI as an interface. This is the first report of the simultaneous analysis of eight oxoisoaporphine alkaloids in Rhizoma Menispermi using a UPLC-MS/MS method; this analysis afforded good linearity, precision, and accuracy. Then, the method was successfully applied to determine the alkaloids in Rhizoma Menispermi from different sources.
[Mh] Termos MeSH primário: Alcaloides/análise
Cromatografia Líquida de Alta Pressão/métodos
Menispermum/química
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão/economia
Limite de Detecção
Modelos Lineares
Rizoma/química
Espectrometria de Massas em Tandem/economia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; VALIDATION STUDIES
[Nm] Nome de substância:
0 (Alkaloids)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:160512
[Lr] Data última revisão:
160512
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150508
[St] Status:MEDLINE
[do] DOI:10.1007/s00216-015-8704-4


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[PMID]:25605184
[Au] Autor:Zhang HF; Wu D; Du JK; Zhang Y; Su YM
[Ad] Endereço:Department of Gastrointestinal Surgery, Harbin Medical University Cancer Hospital, Harbin, Heilongjiang, China E-mail : hongfengzhhf@163.com.
[Ti] Título:Inhibitory effects of phenolic alkaloids of Menispermum dauricum on gastric cancer in vivo.
[So] Source:Asian Pac J Cancer Prev;15(24):10825-30, 2014.
[Is] ISSN:2476-762X
[Cp] País de publicação:Thailand
[La] Idioma:eng
[Ab] Resumo:The present study was conducted to investigate effects and mechanisms of action of phenolic alkaloids of Menispermum dauricum (PAMD) on gastric cancer in vivo. In vitro, cell apoptosis of human gastric cancer cell line SGC-7901 was observed using fluorescence staining. In vivo, a mice model was constructed to observe tumor growth with different doses. Cell apoptosis was examined using flow cytometry and K-RAS protein expression using Western blotting. The mRNA expression of P53, BCL-2, BAX, CASPASE-3, K-RAS was examined by real-time PCR. PAMD significantly suppressed tumor growth in the xenograft model of gastric cancer in a dose- dependent manner (p<0.01). Functionally, PAMD promoted cell apoptosis of the SGC-7901 cells and significantly increased the rate of cell apoptosis of gastric tumor cells (p<0.05). Mechanically, PAMD inhibited the expression of oncogenic K-RAS both at the mRNA and protein levels. In addition, PAMD affected the mRNA expression of the cell apoptosis-related genes (P53, BCL-2, BAX, CASPASE-3). PAMD could suppress gastric tumor growth in vivo, possibly through inhibiting oncogenic K-RAS, and induce cell apoptosis possibly by targeting the cell apoptosis-related genes of P53, BCL-2, BAX, CASPASE-3.
[Mh] Termos MeSH primário: Alcaloides/farmacologia
Apoptose/efeitos dos fármacos
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Menispermum/química
Fenóis/química
Neoplasias Gástricas/tratamento farmacológico
Neoplasias Gástricas/patologia
[Mh] Termos MeSH secundário: Animais
Western Blotting
Proliferação Celular/efeitos dos fármacos
Feminino
Seres Humanos
Técnicas Imunoenzimáticas
Masculino
Camundongos
Camundongos Nus
RNA Mensageiro/genética
Reação em Cadeia da Polimerase em Tempo Real
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Neoplasias Gástricas/genética
Células Tumorais Cultivadas
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Phenols); 0 (RNA, Messenger)
[Em] Mês de entrada:1510
[Cu] Atualização por classe:170308
[Lr] Data última revisão:
170308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150122
[St] Status:MEDLINE


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[PMID]:25252930
[Au] Autor:Sun D; Zhou M; Ying X; Cheng B; Han Y; Nie Y; Hou Y; Bai G
[Ti] Título:Identification of nuclear factor-κB inhibitors in the folk herb Rhizoma Menispermi via bioactivity-based ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry analysis.
[So] Source:BMC Complement Altern Med;14:356, 2014 Sep 25.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Rhizoma Menispermi (RM) is the dried root of Menispermum dauricum DC, which is traditionally used to treat swelling and pain for sore throat, enteritis and rheumatic arthralgia in the clinic, but its bioactive compounds remain unclear. METHODS: In this study, RM extract was administered orally to ICR mice followed by challenging with an intratracheal Pseudomonas aeruginosa suspension. Then mortality, histological features of lung, and inflammatory cytokines were evaluated. RM treatment significantly ameliorated Pseudomonas aeruginosa-induced acute lung inflammation and reduced levels of inflammatory mediators. To screen for potential anti-inflammatory constituents of the RM extract, a simple and rapid method based on ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-Q/TOF MS) coupled with a luciferase reporter assay system to detect nuclear factor-κB (NF-κB) activity was established. RESULTS: Using this system, seven potential NF-κB inhibitors were detected, including sinomenine, norsinoacutin, N-norsinoacutin-ß-D-glucopyranoside, 6-O-methyl-laudanosoline-13-O-glucopyranoside, magnoflorine, laurifloline and dauricinoline. Furthermore, IL-6 and IL-8 assays confirmed the anti-inflammatory effects of these potential NF-κB inhibitors, in which norsinoacutin, 6-O-methyl-laudanosoline-13-O-glucopyranoside laurifloline, dauricinoline and N-norsinoacutin-ß-D-glucopyranoside were revealed as new NF-κB inhibitors. CONCLUSION: This method of UPLC-Q/TOF coupled with the luciferase reporter assay system was initially applied to the study of RM and was demonstrated to represent a simple, rapid and practical approach to screen for anti-inflammatory compounds. This study provided useful results for further investigation on the anti-inflammatory mechanism of RM.
[Mh] Termos MeSH primário: Anti-Inflamatórios/química
Medicamentos de Ervas Chinesas/química
Menispermum/química
NF-kappa B/antagonistas & inibidores
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/isolamento & purificação
Anti-Inflamatórios/farmacologia
Anti-Inflamatórios/uso terapêutico
Cromatografia Líquida de Alta Pressão/métodos
Citocinas/metabolismo
Medicamentos de Ervas Chinesas/isolamento & purificação
Medicamentos de Ervas Chinesas/farmacologia
Medicamentos de Ervas Chinesas/uso terapêutico
Células HEK293
Seres Humanos
Pulmão/efeitos dos fármacos
Pulmão/patologia
Masculino
Espectrometria de Massas/métodos
Camundongos
Camundongos Endogâmicos ICR
Pneumonia/tratamento farmacológico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Cytokines); 0 (Drugs, Chinese Herbal); 0 (NF-kappa B)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:151029
[Lr] Data última revisão:
151029
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140926
[St] Status:MEDLINE
[do] DOI:10.1186/1472-6882-14-356


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[PMID]:24615103
[Au] Autor:Wang JY; Sun S; Liu L; Yang WS
[Ad] Endereço:Department of Pathology, Yanbian University College of Medicine, Yanji, Jilin, China.
[Ti] Título:Induction of apoptosis in human cervical carcinoma Hela cells with active components of Menispermum dauricum.
[So] Source:Genet Mol Res;13(2):3545-52, 2014 Feb 13.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Menispermum dauricum DC possesses a wide range of pharmacological effects. In this study, the mechanism of apoptosis induced by active components of M. dauricum was investigated in the human cervical carcinoma HeLa cell line. HeLa cells were treated with different M. dauricum concentrations over different time periods. The proliferation-inhibitory rate and cytotoxic effect of HeLa cells were measured by using the methyl thiazolyl tetrazolium (MTT) assay, and the apoptotic rate was detected by flow cytometry. Expressions of caspase-9, caspase-8, caspase-3, Bcl-2, and Fas proteins, in the apoptotic pathway, and the expression of nuclear factor-kappa B (NF-κB) were detected by SP immunocytochemistry. The MTT assay showed that active components of M. dauricum could significantly inhibit the growth of HeLa cells in a dose- and time-dependent manner (P<0.01). The Sub-Gl peak was found by flow cytometry, and the maximal apoptosis rate was 24.93%. Immunocytochemistry showed that after treatment with M. dauricum, the expressions of caspase-8, caspase-9, caspase-3, Fas protein, and NF-κB all increased, and the expression of the Bcl-2 protein decreased, with significant differences relative to the control group (P<0.01). Apoptosis in HeLa cells could be induced by active components of M. dauricum through the NF-κB signal transduction pathway and the caspase pathway, which was related to the downregulation of Bcl-2 expression and the upregulation of Fas expression.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Apoptose/efeitos dos fármacos
Extratos Vegetais/farmacologia
Neoplasias do Colo do Útero/tratamento farmacológico
[Mh] Termos MeSH secundário: Proliferação Celular/efeitos dos fármacos
Feminino
Citometria de Fluxo
Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos
Células HeLa
Seres Humanos
Menispermum/química
Extratos Vegetais/química
Transdução de Sinais/efeitos dos fármacos
Neoplasias do Colo do Útero/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Plant Extracts)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:140520
[Lr] Data última revisão:
140520
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140312
[St] Status:MEDLINE
[do] DOI:10.4238/2014.February.13.17


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[PMID]:24378368
[Au] Autor:Dong PL; Han H; Zhang TY; Yang B; Wang QH; Eerdun GW
[Ad] Endereço:Key Laboratory of Chinese Materia Medica of Ministry of Education, Heilongjiang University of Chinese Medicine, Harbin, Heilongjiang 150040, P.R. China.
[Ti] Título:P­glycoprotein inhibition increases the transport of dauricine across the blood­brain barrier.
[So] Source:Mol Med Rep;9(3):985-8, 2014 Mar.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:Dauricine is the major bioactive component isolated from the roots of Menispermum dauricum D.C. The aim of the present study was to investigate the role of P­glycoprotein in the transport of dauricine across the blood­brain barrier by pre­treatment with the P­glycoprotein inhibitor verapamil. Sprague Dawley rats were divided into a verapamil group (pretreated with verapamil at a dose of 20 mg/kg) and a control group (pretreated with the same volume of normal saline). After 90 min, the animals were injected intravenously with dauricine (10 mg/kg). At 15, 30 and 60 min after dauricine administration, the levels of dauricine in the blood and brain were detected by high­performance liquid chromatography. Compared with the control group, the dauricine concentration in the brains of the rats in the verapamil group was significantly increased. Furthermore, the brain­plasma ratio of dauricine in the rats pretreated with verapamil was significantly higher than that of the animals in the control group. However, there was no difference identified between dauricine levels in the plasma of the verapamil and the control groups. The results indicated that dauricine is able to pass the blood­brain barrier, and that P­glycoprotein has an important role in the transportation of dauricine across the blood­brain barrier.
[Mh] Termos MeSH primário: Subfamília B de Transportador de Cassetes de Ligação de ATP/metabolismo
Benzilisoquinolinas/farmacologia
Barreira Hematoencefálica/efeitos dos fármacos
Tetra-Hidroisoquinolinas/farmacologia
[Mh] Termos MeSH secundário: Subfamília B de Transportador de Cassetes de Ligação de ATP/antagonistas & inibidores
Animais
Benzilisoquinolinas/análise
Transporte Biológico/efeitos dos fármacos
Barreira Hematoencefálica/metabolismo
Encéfalo/metabolismo
Cromatografia Líquida de Alta Pressão
Masculino
Menispermum/química
Menispermum/metabolismo
Ratos
Ratos Sprague-Dawley
Tetra-Hidroisoquinolinas/análise
Vasodilatadores/farmacologia
Verapamil/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (ATP Binding Cassette Transporter, Sub-Family B); 0 (Benzylisoquinolines); 0 (Tetrahydroisoquinolines); 0 (Vasodilator Agents); 8QTO90G5W5 (dauricine); CJ0O37KU29 (Verapamil)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140101
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2013.1880


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[PMID]:23399279
[Au] Autor:Lin M; Xia B; Yang M; Gao S; Huo Y; Lou G
[Ad] Endereço:Department of Gynecology, The Third Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
[Ti] Título:Anti-ovarian cancer potential of two acidic polysaccharides from the rhizoma of Menispermum dauricum.
[So] Source:Carbohydr Polym;92(2):2212-7, 2013 Feb 15.
[Is] ISSN:1879-1344
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Two acidic polysaccharides (MDP-A1 and MDP-A2) were isolated from the rhizome of Menispermum dauricum and their apparent molecular weight are 9.1×10(4) and 5.8×10(4) Da, respectively. Both contained glucose, mannose, galactose, arabinose, glucuronic acid and galacturonic acid, but differed in the molar ratio. We also investigated the antitumor activities and mechanisms of MDP-A1 and MDP-A2 in human ovarian carcinoma SKOV3 cells. The MTT assay showed that MDP-A1 and MDP-A2 were able to inhibit cell proliferation of SKOV3 cells in a bell-shaped concentration-response manner, due to a significant increase in the number of apoptotic cells. Furthermore, treatment with MDP-A1 or MDP-A2 caused significant induction of caspase-3 and caspase-8, but slightly affected caspase-9 activity. In addition, a significant reduction in tumor volume was observed in mice treated with MDP-A1 or MDP-A2. Taken together, these results suggested that MDP-A1 and MDP-A2 had a potential application as natural antitumor drugs.
[Mh] Termos MeSH primário: Antineoplásicos/química
Antineoplásicos/farmacologia
Menispermum/química
Neoplasias Ovarianas/patologia
Polissacarídeos/química
Polissacarídeos/farmacologia
Rizoma/química
[Mh] Termos MeSH secundário: Animais
Antineoplásicos/isolamento & purificação
Caspases/metabolismo
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Feminino
Seres Humanos
Camundongos
Polissacarídeos/isolamento & purificação
Ensaios Antitumorais Modelo de Xenoenxerto
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Polysaccharides); EC 3.4.22.- (Caspases)
[Em] Mês de entrada:1308
[Cu] Atualização por classe:130212
[Lr] Data última revisão:
130212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130213
[St] Status:MEDLINE


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[PMID]:23164756
[Au] Autor:Lin M; Xia B; Yang M; Gao S; Huo Y; Lou G
[Ad] Endereço:Department of Gynecology, The Third Affiliated Hospital of Harbin Medical University, Harbin 150081, China.
[Ti] Título:Characterization and antitumor activities of a polysaccharide from the rhizoma of Menispermum dauricum.
[So] Source:Int J Biol Macromol;53:72-6, 2013 Feb.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The rhizome of Menispermum dauricum DC (Menispermaceae) is one of the most commonly used traditional Chinese medicines officially listed in Chinese Pharmacopeia. In present study, we purified a water-soluble polysaccharide (WMDP) from this plant and investigated its physicochemical properties. WMDP was a homogeneous polysaccharide, with an average molecular weight of approximately 3.5×10(4)Da, as determined by high-performance gel-permeation chromatography (HPGPC). Gas chromatography (GC) analysis identified that WMDP was composed of Glc, Gal, Xyl, Rha, Ara and Man in the ratio of 2.45:2.13:1.05:1.29:1.63:1.45. The interreaction between Gongo Red and WMDP in NaOH solutions resulted in the shift of maximum absorption, indicating WMDP had a triple-helix conformation. We also investigated the antitumor activities and mechanisms of WMDP in human ovarian carcinoma SKOV3 cells. The experimental evidence showed that WMDP significantly inhibited cell proliferation and DNA synthesis in SKOV3 cells in a concentration-dependent manner, due to a significant increase in the number of apoptotic cells. Furthermore, treatment with WMDP caused a rapid loss of intracellular glutathione (GSH) content and stimulation of reactive oxygen species (ROS). In addition, nuclear factor-kappa B (NF-κB) in SKOV3 cells received WMDP treatment was inactivated. Taken together, induction of apoptosis on SKOV3 cells by WMDP was mainly associated with ROS production, GSH depletion and NF-κB inactivation.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Medicamentos de Ervas Chinesas/farmacologia
Menispermum/química
Polissacarídeos/farmacologia
Rizoma/química
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/isolamento & purificação
Apoptose/efeitos dos fármacos
Configuração de Carboidratos
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Replicação do DNA/efeitos dos fármacos
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/isolamento & purificação
Glutationa/metabolismo
Seres Humanos
NF-kappa B/metabolismo
Polissacarídeos/química
Polissacarídeos/isolamento & purificação
Ligação Proteica
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Drugs, Chinese Herbal); 0 (NF-kappa B); 0 (Polysaccharides); 0 (Reactive Oxygen Species); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1306
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121121
[St] Status:MEDLINE


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[PMID]:23199717
[Au] Autor:Wang J; Li Y; Zu XB; Chen MF; Qi L
[Ad] Endereço:Department of Urology, Xiangya Hospital, Central South University, Changsha Hunan Province, China. wangjun2538@126.com
[Ti] Título:Dauricine can inhibit the activity of proliferation of urinary tract tumor cells.
[So] Source:Asian Pac J Trop Med;5(12):973-6, 2012 Dec.
[Is] ISSN:2352-4146
[Cp] País de publicação:China
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: To explore the anti-tumor effects of asiatic moonseed rhizome extraction-dauricine on bladder cancer EJ cell strain, prostate cancer PC-3Mcell strain and primary cell culture system. METHODS: The main effective component-phenolic alkaloids ofMenispermum dauricum was extracted and separated from asiatic moonseed rhizome by chemical method. MTT method was used to detect dauricine anti-tumor effect. RESULTS: Dauricine had an obvious proliferation inhibition effect on the main tumor cells in urinary system. The minimum drug sensitivity concentration was between 3.81-5.15 µg/mL, and the inhibition ratio increased with the increase of concentration. CONCLUSIONS: Dauricine, the main effective component extracted from asiatic moonseed rhizome, had a good inhibition effect on tumor cells in urinary system. At the same time, Dauricine has certain inhibition effects on the primary cultured tumor cell.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Benzilisoquinolinas/farmacologia
Tetra-Hidroisoquinolinas/farmacologia
Neoplasias da Bexiga Urinária/tratamento farmacológico
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/isolamento & purificação
Antineoplásicos Fitogênicos/farmacocinética
Benzilisoquinolinas/química
Benzilisoquinolinas/isolamento & purificação
Benzilisoquinolinas/farmacocinética
Linhagem Celular Tumoral
Proliferação Celular/efeitos dos fármacos
Seres Humanos
Menispermum/química
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Rizoma/química
Tetra-Hidroisoquinolinas/química
Tetra-Hidroisoquinolinas/isolamento & purificação
Tetra-Hidroisoquinolinas/farmacocinética
Neoplasias da Bexiga Urinária/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Benzylisoquinolines); 0 (Plant Extracts); 0 (Tetrahydroisoquinolines); 8QTO90G5W5 (dauricine)
[Em] Mês de entrada:1308
[Cu] Atualização por classe:160519
[Lr] Data última revisão:
160519
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121204
[St] Status:MEDLINE



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