Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.756.249 [Categoria DeCS]
Referências encontradas : 29 [refinar]
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  1 / 29 MEDLINE  
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[PMID]:28774427
[Au] Autor:Kumar R; Duffy S; Avery VM; Davis RA
[Ad] Endereço:Griffith Institute for Drug Discovery, Griffith University, Brisbane, QLD 4111, Australia.
[Ti] Título:Synthesis of antimalarial amide analogues based on the plant serrulatane diterpenoid 3,7,8-trihydroxyserrulat-14-en-19-oic acid.
[So] Source:Bioorg Med Chem Lett;27(17):4091-4095, 2017 09 01.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A plant-derived natural product scaffold, 3,7,8-trihydroxyserrulat-14-en-19-oic acid (1) was isolated in high yield from the aerial parts of the endemic Australian desert plant Eremophila microtheca. This scaffold (1) was subsequently used in the generation of a series of new amide analogues via a one-pot mixed anhydride amidation using pivaloyl chloride. The structures of all analogues were characterized using MS, NMR, and UV data. The major serrulatane natural products (1-3), isolated from the plant extract, and all amide analogues (6-15) together with several pivaloylated derivatives of 3,7,8-trihydroxyserrulat-14-en-19-oic acid (16-18) were evaluated for their antimalarial activity against 3D7 (chloroquine sensitive) and Dd2 (chloroquine resistant) Plasmodium falciparum strains, and preliminary cytotoxicity data were also acquired using the human embryonic kidney cell line HEK293. The natural product scaffold (1) did not display any antimalarial activity at 10µM. Replacing the carboxylic acid of 1 with various amides resulted in moderate activity against the P. falciparum 3D7 strain with IC values ranging from 1.25 to 5.65µM.
[Mh] Termos MeSH primário: Amidas/farmacologia
Antimaláricos/farmacologia
Produtos Biológicos/farmacologia
Diterpenos/farmacologia
Eremophila (Planta)/química
Extratos Vegetais/química
Plasmodium falciparum/efeitos dos fármacos
[Mh] Termos MeSH secundário: Amidas/síntese química
Amidas/química
Antimaláricos/síntese química
Antimaláricos/química
Austrália
Produtos Biológicos/síntese química
Produtos Biológicos/química
Sobrevivência Celular/efeitos dos fármacos
Diterpenos/síntese química
Diterpenos/química
Relação Dose-Resposta a Droga
Células HEK293
Seres Humanos
Estrutura Molecular
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (3,7,8-trihydroxyserrulat-14-en-19-oic acid); 0 (Amides); 0 (Antimalarials); 0 (Biological Products); 0 (Diterpenes); 0 (Plant Extracts)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171125
[Lr] Data última revisão:
171125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170805
[St] Status:MEDLINE


  2 / 29 MEDLINE  
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[PMID]:28257200
[Au] Autor:Galappathie S; Edwards DJ; Elliott AG; Cooper MA; Palombo EA; Butler MS; Mahon PJ
[Ad] Endereço:Department of Chemistry and Biotechnology, Faculty of Science, Engineering and Technology, Swinburne University of Technology , Hawthorn 3122, Victoria, Australia.
[Ti] Título:Antibacterial Nerol Cinnamates from the Australian Plant Eremophila longifolia.
[So] Source:J Nat Prod;80(4):1178-1181, 2017 Apr 28.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two new antimicrobial agents, neryl ferulate (1) and neryl p-coumarate (2), were identified using bioassay-guided isolation from the leaves of Eremophila longifolia, which is a medicinal plant used by some Australian Aboriginal communities. Although gradual autoxidation of the nerol subunit hindered the initial attempts to purify and characterize 1 and 2, it was found that the autoxidation could be stopped through storage under argon at -20 °C. Biological evaluation showed that neryl ferulate (1) had moderate activity against various Gram-positive bacteria, while neryl p-coumarate (2) was active only against Enterococcus faecium.
[Mh] Termos MeSH primário: Antibacterianos/isolamento & purificação
Antibacterianos/farmacologia
Anti-Infecciosos/isolamento & purificação
Anti-Infecciosos/farmacologia
Cinamatos/isolamento & purificação
Cinamatos/farmacologia
Ácidos Cumáricos/isolamento & purificação
Ácidos Cumáricos/farmacologia
Eremophila (Planta)/química
Plantas Medicinais/química
[Mh] Termos MeSH secundário: Antibacterianos/química
Anti-Infecciosos/química
Austrália
Cinamatos/química
Ácidos Cumáricos/química
Bactérias Gram-Positivas/efeitos dos fármacos
Células Hep G2
Seres Humanos
Testes de Sensibilidade Microbiana
Estrutura Molecular
Folhas de Planta/química
Terpenos/química
Terpenos/isolamento & purificação
Terpenos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Anti-Infective Agents); 0 (Cinnamates); 0 (Coumaric Acids); 0 (Terpenes); L837108USY (geraniol)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b00888


  3 / 29 MEDLINE  
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[PMID]:28162767
[Au] Autor:Kracht ON; Ammann AC; Stockmann J; Wibberg D; Kalinowski J; Piotrowski M; Kerr R; Brück T; Kourist R
[Ad] Endereço:Junior Research Group for Microbial Biotechnology, Ruhr-Universität Bochum, Universitätsstraße 150, 44780 Bochum, Germany.
[Ti] Título:Transcriptome profiling of the Australian arid-land plant Eremophila serrulata (A.DC.) Druce (Scrophulariaceae) for the identification of monoterpene synthases.
[So] Source:Phytochemistry;136:15-22, 2017 Apr.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Plant terpenoids are a large and highly diverse class of metabolites with an important role in the immune defense. They find wide industrial application as active pharmaceutical ingredients, aroma and fragrance compounds. Several Eremophila sp. derived terpenoids have been documented. To elucidate the terpenoid metabolism, the transcriptome of juvenile and mature Eremophila serrulata (A.DC.) Druce (Scrophulariaceae) leaves was sequenced and a transcript library was generated. We report on the first transcriptomic dataset of an Eremophila plant. IlluminaMiSeq sequencing (2 × 300 bp) revealed 7,093,266 paired reads, which could be assembled to 34,505 isogroups. To enable detection of terpene biosynthetic genes, leaves were separately treated with methyl jasmonate, a well-documented inducer of plant secondary metabolites. In total, 21 putative terpene synthase genes were detected in the transcriptome data. Two terpene synthase isoenzymatic genes, termed ES01 and ES02, were successfully expressed in E. coli. The resulting proteins catalyzed the conversion of geranyl pyrophosphate, the universal substrate of monoterpene synthases to myrcene and Z-(b)-ocimene, respectively. The transcriptomic data and the discovery of the first terpene synthases from Eremophila serrulata are the initial step for the understanding of the terpene metabolism in this medicinally important plant genus.
[Mh] Termos MeSH primário: Alquil e Aril Transferases/metabolismo
Terpenos/metabolismo
[Mh] Termos MeSH secundário: Acetatos
Alcenos
Austrália
Ciclopentanos
Eremophila (Planta)
Escherichia coli/genética
Perfilação da Expressão Gênica
Liases Intramoleculares/metabolismo
Monoterpenos
Oxilipinas
Proteínas de Plantas/metabolismo
Fosfatos de Poli-Isoprenil/metabolismo
Scrophulariaceae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Alkenes); 0 (Cyclopentanes); 0 (Monoterpenes); 0 (Oxylipins); 0 (Plant Proteins); 0 (Polyisoprenyl Phosphates); 0 (Terpenes); 3M39CZS25B (myrcene); 763-10-0 (geranyl pyrophosphate); 900N171A0F (methyl jasmonate); EC 2.5.- (Alkyl and Aryl Transferases); EC 2.5.1.- (terpene synthase); EC 5.5.- (Intramolecular Lyases); EC 5.5.- (pinene cyclase I)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170207
[St] Status:MEDLINE


  4 / 29 MEDLINE  
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[PMID]:27395952
[Au] Autor:Taylor GS; Fagan-Jeffries EP; Austin AD
[Ad] Endereço:Australian Centre for Evolutionary Biology & Biodiversity, and Department of Genetics & Evolution, School of Biological Sciences, University of Adelaide, South Australia 5005, Australia.; Email: Gary.Taylor@adelaide.edu.au.
[Ti] Título:A new genus and twenty new species of Australian jumping plant-lice (Psylloidea: Triozidae) from Eremophila and Myoporum (Scrophulariaceae: Myoporeae).
[So] Source:Zootaxa;4073(1):1-84, 2016 Feb 05.
[Is] ISSN:1175-5334
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:The Triozidae is a diverse, cosmopolitan family of jumping plant-lice (Hemiptera: Psylloidea) from an exceptionally diverse range of plant families, but with few described Australian species. As a direct outcome of the Australian Biological Resources Study Bush Blitz species discovery program, many new Psylloidea from novel host plants in remote localities have been revealed. In this study a new genus Myotrioza Taylor gen. nov. and 20 new species are described from southern and central Australia which also establishes the first host plant records from Eremophila and Myoporum (Scrophulariaceae: Myoporeae). New species, delineated using a combination of morphological and mitochondrial COI sequence data, are: Myotrioza clementsiana sp. nov., M. darwinensis sp. nov., M. desertorum sp. nov., M. eremi sp. nov., M. eremophili sp. nov., M. flindersiana sp.nov., M. gawlerensis sp. nov., M. insularis sp. nov., M. interioris sp. nov., M. interstantis sp. nov., M. longifoliae sp. nov., M. markmitchelli sp. nov., M. myopori sp. nov., M. oppositifoliae sp. nov., M. pantonii sp. nov., M. platycarpi sp. nov., M. remota sp. nov., M. scopariae sp. nov., M. serrulatae sp. nov., and M. telowiensis sp. nov. Genetic divergence data, host associations, biogeographic data, diagnoses and a key to species are presented. Myotrioza appears to be particularly diverse in ephemeral southern Australia, especially in inland Western Australia and South Australia, matching regions of high diversity of the host genera; some species are likely to be short range endemics.
[Mh] Termos MeSH primário: Afídeos/classificação
Eremophila (Planta)/parasitologia
Myoporum/parasitologia
[Mh] Termos MeSH secundário: Distribuição Animal
Estruturas Animais/anatomia & histologia
Estruturas Animais/crescimento & desenvolvimento
Animais
Afídeos/anatomia & histologia
Afídeos/genética
Afídeos/crescimento & desenvolvimento
Tamanho Corporal
Ecossistema
Feminino
Masculino
Tamanho do Órgão
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170203
[Lr] Data última revisão:
170203
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160711
[St] Status:MEDLINE
[do] DOI:10.11646/zootaxa.4073.1.1


  5 / 29 MEDLINE  
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[PMID]:26636180
[Au] Autor:Mon HH; Christo SN; Ndi CP; Jasieniak M; Rickard H; Hayball JD; Griesser HJ; Semple SJ
[Ad] Endereço:Sansom Institute for Health Research, School of Pharmacy and Medical Sciences, University of South Australia , Adelaide, SA 5000, Australia.
[Ti] Título:Serrulatane Diterpenoid from Eremophila neglecta Exhibits Bacterial Biofilm Dispersion and Inhibits Release of Pro-inflammatory Cytokines from Activated Macrophages.
[So] Source:J Nat Prod;78(12):3031-40, 2015 Dec 24.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The purpose of this study was to assess the biofilm-removing efficacy and inflammatory activity of a serrulatane diterpenoid, 8-hydroxyserrulat-14-en-19-oic acid (1), isolated from the Australian medicinal plant Eremophila neglecta. Biofilm breakup activity of compound 1 on established Staphylococcus epidermidis and Staphylococcus aureus biofilms was compared to the antiseptic chlorhexidine and antibiotic levofloxacin. In a time-course study, 1 was deposited onto polypropylene mesh to mimic a wound dressing and tested for biofilm removal. The ex-vivo cytotoxicity and effect on lipopolysaccharide-induced pro-inflammatory cytokine release were studied in mouse primary bone-marrow-derived macrophage (BMDM) cells. Compound 1 was effective in dispersing 12 h pre-established biofilms with a 7 log10 reduction of viable bacterial cell counts, but was less active against 24 h biofilms (approximately 2 log10 reduction). Compound-loaded mesh showed dosage-dependent biofilm-removing capability. In addition, compound 1 displayed a significant inhibitory effect on tumor necrosis factor alpha (TNF-α) and interleukin-6 (IL-6) secretion from BMDM cells, but interleukin-1 beta (IL-1ß) secretion was not significant. The compound was not cytotoxic to BMDM cells at concentrations effective in removing biofilm and lowering cytokine release. These findings highlight the potential of this serrulatane diterpenoid to be further developed for applications in wound management.
[Mh] Termos MeSH primário: Antibacterianos/isolamento & purificação
Antibacterianos/farmacologia
Biofilmes/efeitos dos fármacos
Diterpenos/isolamento & purificação
Diterpenos/farmacologia
Eremophila (Planta)/química
Plantas Medicinais/química
[Mh] Termos MeSH secundário: Animais
Antibacterianos/química
Austrália
Sobrevivência Celular/efeitos dos fármacos
Citocinas/biossíntese
Citocinas/efeitos dos fármacos
Citocinas/farmacologia
Diterpenos/química
Relação Dose-Resposta a Droga
Interleucina-1beta/efeitos dos fármacos
Interleucina-6
Levofloxacino/farmacologia
Lipopolissacarídeos/farmacologia
Macrófagos/efeitos dos fármacos
Camundongos
Testes de Sensibilidade Microbiana
Estrutura Molecular
Scrophulariaceae
Infecções Estafilocócicas/microbiologia
Staphylococcus aureus/efeitos dos fármacos
Staphylococcus epidermidis/efeitos dos fármacos
Fator de Necrose Tumoral alfa/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (8-hydroxyserrulat-14-en-19-oic acid); 0 (Anti-Bacterial Agents); 0 (Cytokines); 0 (Diterpenes); 0 (Interleukin-1beta); 0 (Interleukin-6); 0 (Lipopolysaccharides); 0 (Tumor Necrosis Factor-alpha); 6GNT3Y5LMF (Levofloxacin)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:151224
[Lr] Data última revisão:
151224
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151205
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.5b00833


  6 / 29 MEDLINE  
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[PMID]:26053015
[Au] Autor:Tan AC; Konczak I; Li G; Roufogalis BD; Sekhon B; Sze DM
[Ti] Título:An ethnopharmacological approach to the preliminary screening of native Australian herbal medicines for anticancer activity.
[So] Source:J Complement Integr Med;12(3):245-9, 2015 Sep.
[Is] ISSN:1553-3840
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Five plants used traditionally by Australian Aboriginals and two edible native Australian fruits have been investigated for anticancer activity. The aim was to identify native Australian herbal medicines which displayed anticancer activity, with cytotoxicity to cancer cells but sparing or even proliferating normal immunological cells, and subsequently provide potentially new anticancer drug leads. METHODS: Extracts and derived fractions were assayed for cell viability against a multiple myeloma cell line, RPMI-8226, in comparison to the peripheral blood mononuclear cells (PBMC) representing normal human immunological cells. RESULTS: None of the crude extracts exhibited the desirable differential activity; however, following further fractionation of the Eremophila duttonii F. Muell. (Myoporaceae) extract, one fraction (termed F01) exhibited a greater cytotoxicity to the cancer cell line than to the normal cells. CONCLUSIONS: One fraction may potentially contain valuable compounds which may be useful for further investigation. This may focus on the identification of the bioavailable purified compounds present within these fractions or by detailed delineation of the related mechanisms of action.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Linhagem Celular Tumoral/efeitos dos fármacos
Medicina Herbária
Leucócitos Mononucleares/efeitos dos fármacos
Mieloma Múltiplo/tratamento farmacológico
Extratos Vegetais/farmacologia
Plantas Medicinais/química
[Mh] Termos MeSH secundário: Acacia/química
Alstonia/química
Austrália
Sobrevivência Celular/efeitos dos fármacos
Eremophila (Planta)/química
Frutas/química
Seres Humanos
Medicina Tradicional
Grupo com Ancestrais Oceânicos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:170328
[Lr] Data última revisão:
170328
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150609
[St] Status:MEDLINE


  7 / 29 MEDLINE  
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[PMID]:25002548
[Au] Autor:Kurekci C; Al Jassim R; Hassan E; Bishop-Hurley SL; Padmanabha J; McSweeney CS
[Ad] Endereço:School of Agriculture and Food Sciences, The University of Queensland, Gatton, QLD 4343, Australia CSIRO Animal, Food and Health Sciences, Queensland Biosciences Precinct, 306 Carmody Road, St Lucia, QLD 4067, Australia Faculty of Veterinary Medicine, Department of Food Hygiene and Technology, Musta
[Ti] Título:Effects of feeding plant-derived agents on the colonization of Campylobacter jejuni in broiler chickens.
[So] Source:Poult Sci;93(9):2337-46, 2014 Sep.
[Is] ISSN:0032-5791
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this work was to test the potential use of plant-derived extracts and compounds to control Campylobacter jejuni in broiler chickens. Over a 7-wk feeding period, birds were fed a commercial diet with or without plant extracts (Acacia decurrens, Eremophila glabra), essential oil [lemon myrtle oil (LMO)], plant secondary compounds [terpinene-4-ol and α-tops (including α-terpineol, cineole, and terpinene-4-ol)], and the antibiotic virginiamycin. Traditional culture and real-time quantitative PCR techniques were used to enumerate the numbers of C. jejuni in chicken fecal and cecal samples. In addition, BW and feed intake were recorded weekly for the calculation of BW gain and feed conversion ratio. The mean log10 counts of C. jejuni were similar (P > 0.05) across treatments. However, significantly lower levels of fecal Campylobacter counts (P < 0.05) were recorded at d 41 for the α-tops treatment by culture methods. No differences (P > 0.05) in BW gain were obtained for dietary supplementation, except for the E. glabra extract, which had a negative impact (P < 0.001) on BW, resulting in sporadic death. Results from this study suggest that supplemental natural compounds used in the current study did not reduce the shedding of C. jejuni to desired levels.
[Mh] Termos MeSH primário: Infecções por Campylobacter/veterinária
Campylobacter jejuni/efeitos dos fármacos
Galinhas
Suplementos Nutricionais
Extratos Vegetais
Doenças das Aves Domésticas/prevenção & controle
Virginiamicina/farmacologia
[Mh] Termos MeSH secundário: Acacia/química
Ração Animal/análise
Animais
Antibacterianos/farmacologia
Derrame de Bactérias/efeitos dos fármacos
Infecções por Campylobacter/prevenção & controle
Ceco/microbiologia
Galinhas/crescimento & desenvolvimento
Dieta/veterinária
Eremophila (Planta)/química
Fezes/microbiologia
Masculino
Doenças das Aves Domésticas/microbiologia
Distribuição Aleatória
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Plant Extracts); 11006-76-1 (Virginiamycin)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:140826
[Lr] Data última revisão:
140826
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140709
[St] Status:MEDLINE
[do] DOI:10.3382/ps.2014-03950


  8 / 29 MEDLINE  
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[PMID]:24874947
[Au] Autor:Sadgrove NJ; Jones GL
[Ad] Endereço:Pharmaceuticals and Nutraceuticals Group, Centre for Bioactive Discovery in Health and Ageing, School of Science and Technology, University of New England, Armidale, NSW 2351, Australia. Electronic address: nsadgrov@une.edu.au.
[Ti] Título:Cytogeography of essential oil chemotypes of Eremophila longifolia F. Muell (Scrophulariaceae).
[So] Source:Phytochemistry;105:43-51, 2014 Sep.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Previous studies have demonstrated that the widely distributed desert plant Eremophila longifolia has at least six geographically defined essential oil chemotypes. The focus of the present study is to extend and enhance information concerning known chemotypes and to investigate the involvement of cell nuclei ploidy in this variation. Forty field collected specimens of E. longifolia were taken from most of the mainland states of Australia then subjected to hydrodistillation to produce essential oils, which were then chemically characterised. Ploidy was determined using relative fluorescence of cell nuclei stained with propidium iodide, measured in a flow cytometer. Using principal component analysis (PCA), at least three essential oil chemotypes, in addition to the six already described, were identified in the present study. Previously described high yielding essential oil chemotypes were also characterised in terms of diploidy. For the first time diploid populations were identified in New South Wales, correlating with high yielding isomenthone/menthone and karahanaenone chemotypes. Furthermore, the separate diploid population previously described from Western Australia was demonstrated to be the safrole/methyl eugenol type, which is restricted to a small geographic range in far north-west Western Australia (Murchison District). All other chemotypes were shown to be tetraploid, including apparently randomly emerging individuals, representative of chemotypes producing low yields of isomenthone/menthone and karahanaenone similar in composition to the high yielding diploid types.
[Mh] Termos MeSH primário: Eremophila (Planta)/química
Óleos Voláteis/química
Scrophulariaceae/química
[Mh] Termos MeSH secundário: Austrália
Eugenol/análogos & derivados
Eugenol/análise
Eugenol/química
Seres Humanos
Mentol/análise
Mentol/química
Estrutura Molecular
Análise de Componente Principal
Safrol/análise
Safrol/química
Scrophulariaceae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oils, Volatile); 1490-04-6 (Menthol); 29T9VA6R7M (methyleugenol); 3T8H1794QW (Eugenol); 9NH5J4V8FN (menthone); RSB34337V9 (Safrole)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140531
[St] Status:MEDLINE


  9 / 29 MEDLINE  
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[PMID]:24419660
[Au] Autor:Zaferanloo B; Quang TD; Daumoo S; Ghorbani MM; Mahon PJ; Palombo EA
[Ad] Endereço:Faculty of Life and Social Sciences, Environment and Biotechnology Centre, Swinburne University of Technology, Hawthorn, VIC, 3122, Australia.
[Ti] Título:Optimization of protease production by endophytic fungus, Alternaria alternata, isolated from an Australian native plant.
[So] Source:World J Microbiol Biotechnol;30(6):1755-62, 2014 Jun.
[Is] ISSN:1573-0972
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Endophytes are recognised as potential sources of novel secondary metabolites, including enzymes and drugs, with applications in medicine, agriculture and industry. There is a growing need for new enzymes, including proteases, for use in industry that can function under a variety of conditions. In this study, three fungal endophytes (Alternaria alternata, Phoma herbarum and an unclassified fungus), were isolated from the Australian native plant, Eremophilia longifolia, and assessed for production of proteases. The lyophilised growth media obtained after fungal fermentation were analysed for protease production using enzyme activity assays. Protease production was optimised by assessing the effects of temperature, pH, carbon source and nitrogen source on activity. A. alternata showed the greatest protease activity in a wide range of pH (3-9). The broadest activity between 9 and 50 °C was observed at pH 7, suggesting a neutral protease. Overall, the optimum conditions were 37 °C and pH 7 with a maximum specific activity value of 69.86 BAEE units/mg. The characteristics demonstrated by this fungal endophyte showed that it is a potential source of an enzyme with particular application in the dairy industry. However, further studies of the tolerance to higher temperatures and pH will indicate whether the enzyme is suitable to such applications.
[Mh] Termos MeSH primário: Alternaria/enzimologia
Endófitos/enzimologia
Eremophila (Planta)/microbiologia
Proteínas Fúngicas/metabolismo
Peptídeo Hidrolases/metabolismo
[Mh] Termos MeSH secundário: Alternaria/química
Alternaria/genética
Alternaria/isolamento & purificação
Austrália
Meios de Cultura/química
Meios de Cultura/metabolismo
Endófitos/química
Endófitos/genética
Endófitos/isolamento & purificação
Estabilidade Enzimática
Fermentação
Proteínas Fúngicas/química
Proteínas Fúngicas/genética
Temperatura Alta
Concentração de Íons de Hidrogênio
Peptídeo Hidrolases/química
Peptídeo Hidrolases/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Culture Media); 0 (Fungal Proteins); EC 3.4.- (Peptide Hydrolases)
[Em] Mês de entrada:1412
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140115
[St] Status:MEDLINE
[do] DOI:10.1007/s11274-014-1598-z


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[PMID]:24225531
[Au] Autor:Li X; Durmic Z; Liu S; McSweeney CS; Vercoe PE
[Ad] Endereço:School of Animal Biology, The University of Western Australia, Crawley, WA 6009, Australia; Future Farm Industries CRC, The University of Western Australia, Crawley, WA 6009, Australia. Electronic address: xixili.r@gmail.com.
[Ti] Título:Eremophila glabra reduces methane production and methanogen populations when fermented in a Rusitec.
[So] Source:Anaerobe;29:100-7, 2014 Oct.
[Is] ISSN:1095-8274
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Eremophila glabra Juss. (Scrophulariaceae), a native Australian shrub, has been demonstrated to have low methanogenic potential in a batch in vitro fermentation system. The present study aimed to test longer-term effects of E. glabra on rumen fermentation characteristics, particularly methane production and the methanogen population, when included as a component of a fermentation substrate in an in vitro continuous culture system (Rusitec). E. glabra was included at 150, 250, 400 g/kg DM (EG15, EG25, and EG40) with an oaten chaff and lupin-based substrate (control). Overall, the experiment lasted 33 days, with 12 days of acclimatization, followed by two periods during which fermentation characteristics (total gas, methane and VFA productions, dry matter disappearance, pH) were measured. The number of copies of genes specifically associated with total bacteria and cellulolytic bacteria (16S rRNA gene) and total ruminal methanogenic archaeal organisms (the methyl coenzyme M reductase A gene (mcrA)) was also measured during this time using quantitative real-time PCR. Total gas production, methane and volatile fatty acid concentrations were significantly reduced with addition of E. glabra. At the end of the experiment, the overall methane reduction was 32% and 45% for EG15 and EG25 respectively, compared to the control, and the reduction was in a dose-dependent manner. Total bacterial numbers did not change, but the total methanogen population decreased by up to 42.1% (EG40) when compared to the control substrate. The Fibrobacter succinogenes population was reduced at all levels of E. glabra, while Ruminococcus albus was reduced only by EG40. Our results indicate that replacing a portion of a fibrous substrate with E. glabra maintained a significant reduction in methane production and methanogen populations over three weeks in vitro, with some minor inhibition on overall fermentation at the lower inclusion levels.
[Mh] Termos MeSH primário: Eremophila (Planta)/metabolismo
Metano/biossíntese
Consórcios Microbianos/genética
Oxirredutases/genética
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Animais
Avena/metabolismo
Técnicas de Cultura Celular por Lotes/métodos
Biomarcadores/metabolismo
Reatores Biológicos
Euryarchaeota/genética
Euryarchaeota/crescimento & desenvolvimento
Euryarchaeota/metabolismo
Fermentação
Fibrobacter/genética
Fibrobacter/crescimento & desenvolvimento
Fibrobacter/metabolismo
Concentração de Íons de Hidrogênio
Pressão
Reação em Cadeia da Polimerase em Tempo Real
Rúmen/microbiologia
Ruminantes
Ruminococcus/genética
Ruminococcus/crescimento & desenvolvimento
Ruminococcus/metabolismo
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biomarkers); 0 (RNA, Ribosomal, 16S); EC 1.- (Oxidoreductases); EC 2.8.4.1 (methyl coenzyme M reductase); OP0UW79H66 (Methane)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131115
[St] Status:MEDLINE



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