Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.765.500 [Categoria DeCS]
Referências encontradas : 143 [refinar]
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[PMID]:29334931
[Au] Autor:Dzotam JK; Simo IK; Bitchagno G; Celik I; Sandjo LP; Tane P; Kuete V
[Ad] Endereço:Department of Biochemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon.
[Ti] Título:In vitro antibacterial and antibiotic modifying activity of crude extract, fractions and 3',4',7-trihydroxyflavone from Myristica fragrans Houtt against MDR Gram-negative enteric bacteria.
[So] Source:BMC Complement Altern Med;18(1):15, 2018 Jan 15.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Nutmeg is the seed kernel inside the fruit of Myristica fragrans Houtt. (Myristicaceae). It possesses various pharmacological activities but is used in Cameroon only for its flavor in making cakes. The present study thus aimed to investigate the in vitro antibacterial activity and antibiotic modifying activities of crude seed kernel methanol extract (MFS), fractions (MFSa-e) as well as 3',4',7-trihydroxyflavone from Myristica fragrans against a panel of multi-drug resistant (MDR) Gram-negative bacteria. METHODS: The modified rapid p-iodonitrotetrazolium chloride (INT) colorimetric assay was used to determine the Minimal Inhibitory Concentration (MIC) and Minimal Bactericidal Concentration (MBC) on the tested bacteria, as well as those of antibiotics in association with the extract and/or isolated compound. Column chromatography was used for the fractionation and purification of the seed kernel extract whilst the chemical structures of compounds were determined using spectroscopic techniques. RESULTS: Phytochemical investigations lead to the isolation of 3',4',7-trihydroxyflavone from the fraction MFSb. The crude extract showed antibacterial activity with MICs ranging from 32 to 1024 µg/mL on the majority of the 29 tested Gram-negative bacterial strains. Fraction MFSb inhibited the growth of 100% (29/29) of the tested bacterial strains, as well as the compound 3',4',7-trihydroxyflavone (12/12) with a MIC values ranging from 32 to 1024 µg/mL, and 4 to 128 µg/mL respectively. The lowest MIC value (4 µg/mL) was recorded with 3',4',7-trihydroxyflavone against Providencia stuartii ATCC299645 as well as the best MBC value (16 µg/mL) against the same strain. In the presence of Phenylalanine-Arginine-ß-Naphthylamide (PAßN), an efflux pumps inhibitor, the activity of the extract increased on 73.33% (11/15) meanwhile that of 3',4',7-trihydroxyflavone increased on 100% tested bacteria. The compound 3',4',7-trihydroxyflavone potentiated the activity of antibiotics in the majority of the tested bacterial strains. CONCLUSION: The results of the present work provide additional information on the use of nutmeg and it major antibacterial component, 3',4',7-trihydroxyflavone, as a potential drug in the treatment of bacterial infections including multidrug resistant phenotypes.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Flavonoides/farmacologia
Bactérias Gram-Negativas/efeitos dos fármacos
Myristica fragrans/química
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Camarões
Cloranfenicol/farmacologia
Farmacorresistência Bacteriana Múltipla/efeitos dos fármacos
Testes de Sensibilidade Microbiana
Sementes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (3',4',7-trihydroxyflavone); 0 (Anti-Bacterial Agents); 0 (Flavonoids); 0 (Plant Extracts); 66974FR9Q1 (Chloramphenicol)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180216
[Lr] Data última revisão:
180216
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180117
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-018-2084-1


  2 / 143 MEDLINE  
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[PMID]:28470497
[Au] Autor:Balakrishnan S; Sivaji I; Kandasamy S; Duraisamy S; Kumar NS; Gurusubramanian G
[Ad] Endereço:Department of Medical Microbiology, College of Health and Medical Sciences, Haramaya University, P.O. Box 235, Harar, Ethiopia. nbsenthilkumar@gmail.com.
[Ti] Título:Biosynthesis of silver nanoparticles using Myristica fragrans seed (nutmeg) extract and its antibacterial activity against multidrug-resistant (MDR) Salmonella enterica serovar Typhi isolates.
[So] Source:Environ Sci Pollut Res Int;24(17):14758-14769, 2017 Jun.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Biosynthesis of nanoparticles has received increasing attention due its effective mode of action, eco-friendly preparation methodology, and less cytotoxicity. In the present study, silver nanoparticles (AgNPs) from aqueous seed extract of Myristica fragrans (nutmeg) were characterized. Gas chromatography-mass spectrometry (GC-MS) analysis revealed the presence of bioactive components acts as effective in reducing and capping agents for converting AgNO to AgNPs. The UV-Vis absorption spectrum of the biologically reduced reaction mixture showed the surface plasmon peak at 420 nm, which is the characteristic peak of AgNPs. The functional molecules present in the M. fragrans seed extract and their interaction with the AgNPs were identified by the Fourier transform infrared spectroscopy (FT-IR) analysis. X-ray diffraction (XRD) analysis confirmed the face-centered cubic crystalline structure of metallic silver nanoparticle and diameter was calculated using Scherrer's equation. Transmission electron microscope (TEM) image showed spherical shaped particles with an average size of 25 nm. The scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) confirmed the presence of elemental silver. The antibacterial activity of biosynthesized AgNPs was evaluated against multidrug-resistant (MDR) Salmonella enterica serovar Typhi (S. Typhi) according to agar well diffusion, MIC (minimum inhibitory concentration), and IC (inhibitory concentration 50%). The results confirm that bacterial growth was significantly reduced in a dose-dependent manner. Further, the cytotoxic effect of biosynthesized AgNPs on rat spleenocytes was analyzed. Thus, it is suggested that the nutmeg-biosynthesized AgNPs could be a lead drug and used effectively to control the MDR S. Typhi, thereby reducing public health issues and environmental pollution.
[Mh] Termos MeSH primário: Antibacterianos
Nanopartículas Metálicas
Myristica fragrans
[Mh] Termos MeSH secundário: Animais
Resistência a Múltiplos Medicamentos
Ratos
Salmonella typhi/efeitos dos fármacos
Sementes
Prata
Espectroscopia de Infravermelho com Transformada de Fourier
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 3M4G523W1G (Silver)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171215
[Lr] Data última revisão:
171215
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170505
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-017-9065-7


  3 / 143 MEDLINE  
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[PMID]:28764063
[Au] Autor:Martinez-Sena MT; de la Guardia M; Esteve-Turrillas FA; Armenta S
[Ad] Endereço:Department of Analytical Chemistry, University of Valencia, 50th Dr. Moliner St., 46100 Burjassot, Spain.
[Ti] Título:Hard cap espresso extraction and liquid chromatography determination of bioactive compounds in vegetables and spices.
[So] Source:Food Chem;237:75-82, 2017 Dec 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new analytical procedure, based on liquid chromatography with diode array and fluorescence detection, has been proposed for the determination of bioactive compounds in vegetables and spices after hard cap espresso extraction. This novel extraction system has been tested for the determination of capsaicin and dihydrocapsaicin from fresh chilli and sweet pepper, piperine from ground pepper, curcumin from turmeric and curry, and myristicin from nutmeg. Extraction efficiency was evaluated by using acetonitrile:water and ethanol:water mixtures. The proposed method allows the extraction of samples with 100mL of 60% (v/v) ethanol in water. The obtained limits of quantification for the proposed procedure ranged from 0.07 to 0.30mgg and results were statistically comparable with those obtained by ultrasound assisted extraction. Hard cap espresso machines offer a fast, effective and quantitative tool for the extraction of bioactive compounds from food samples with an extraction time lower than 30s, using a global available and low cost equipment.
[Mh] Termos MeSH primário: Especiarias
Verduras
[Mh] Termos MeSH secundário: Capsicum
Cromatografia Líquida
Myristica fragrans
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE


  4 / 143 MEDLINE  
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[PMID]:28551100
[Au] Autor:Park JY; Hwan Lim S; Ram Kim B; Jae Jeong H; Kwon HJ; Song GY; Bae Ryu Y; Song Lee W
[Ad] Endereço:Natural Product Materials Research Center, Korea Research Institute of Bioscience and Biotechnology, Jeongeup 56212, Republic of Korea.
[Ti] Título:Sialidase inhibitory activity of diarylnonanoid and neolignan compounds extracted from the seeds of Myristica fragrans.
[So] Source:Bioorg Med Chem Lett;27(14):3060-3064, 2017 07 15.
[Is] ISSN:1464-3405
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Sialidases are key virulence factors that remove sialic acid from host cell surface glycans, thus unmasking receptors to facilitate bacterial adherence and colonization. In this study, we report the isolation and characterization of novel inhibitors of the Streptococcus pneumoniae sialidases NanA, NanB, and NanC from Myristica fragrans seeds. Of the isolated compounds (1-12), malabaricone C showed the most pneumococcal sialidases inhibition (IC of 0.3µM for NanA, 3.6µM for NanB, and 2.9µM for NanC). These results suggested that malabaricone C and neolignans could be potential agents for combating S. pneumoniae infection agents.
[Mh] Termos MeSH primário: Inibidores Enzimáticos/farmacologia
Lignanas/farmacologia
Myristica fragrans/química
Neuraminidase/antagonistas & inibidores
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Ativação Enzimática/efeitos dos fármacos
Inibidores Enzimáticos/química
Inibidores Enzimáticos/isolamento & purificação
Concentração Inibidora 50
Cinética
Lignanas/química
Lignanas/isolamento & purificação
Myristica fragrans/metabolismo
Neuraminidase/metabolismo
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Isoformas de Proteínas/antagonistas & inibidores
Isoformas de Proteínas/metabolismo
Resorcinóis/síntese química
Resorcinóis/isolamento & purificação
Resorcinóis/farmacologia
Sementes/química
Sementes/metabolismo
Streptococcus pneumoniae/efeitos dos fármacos
Streptococcus pneumoniae/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Enzyme Inhibitors); 0 (Lignans); 0 (Plant Extracts); 0 (Protein Isoforms); 0 (Resorcinols); 63335-25-1 (malabaricone C); EC 3.2.1.18 (Neuraminidase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171124
[Lr] Data última revisão:
171124
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170529
[St] Status:MEDLINE


  5 / 143 MEDLINE  
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[PMID]:28405165
[Au] Autor:Musa SH; Basri M; Fard Masoumi HR; Shamsudin N; Salim N
[Ad] Endereço:Department of Chemistry, Faculty of Science.
[Ti] Título:Enhancement of physicochemical properties of nanocolloidal carrier loaded with cyclosporine for topical treatment of psoriasis: in vitro diffusion and in vivo hydrating action.
[So] Source:Int J Nanomedicine;12:2427-2441, 2017.
[Is] ISSN:1178-2013
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Psoriasis is a chronic autoimmune disease that cannot be cured. It can however be controlled by various forms of treatment, including topical, systemic agents, and phototherapy. Topical treatment is the first-line treatment and favored by most physicians, as this form of therapy has more patient compliance. Introducing a nanoemulsion for transporting cyclosporine as an anti-inflammatory drug to an itchy site of skin disease would enhance the effectiveness of topical treatment for psoriasis. The addition of nutmeg and virgin coconut-oil mixture, with their unique properties, could improve cyclosporine loading and solubility. A high-shear homogenizer was used in formulating a cyclosporine-loaded nanoemulsion. A D-optimal mixture experimental design was used in the optimization of nanoemulsion compositions, in order to understand the relationships behind the effect of independent variables (oil, surfactant, xanthan gum, and water content) on physicochemical response (particle size and polydispersity index) and rheological response (viscosity and -value). Investigation of these variables suggests two optimized formulations with specific oil (15% and 20%), surfactant (15%), xanthan gum (0.75%), and water content (67.55% and 62.55%), which possessed intended responses and good stability against separation over 3 months' storage at different temperatures. Optimized nanoemulsions of pH 4.5 were further studied with all types of stability analysis: physical stability, coalescence-rate analysis, Ostwald ripening, and freeze-thaw cycles. In vitro release proved the efficacy of nanosize emulsions in carrying cyclosporine across rat skin and a synthetic membrane that best fit the Korsmeyer-Peppas kinetic model. In vivo skin analysis towards healthy volunteers showed a significant improvement in the stratum corneum in skin hydration.
[Mh] Termos MeSH primário: Ciclosporina/administração & dosagem
Fármacos Dermatológicos/administração & dosagem
Emulsões/química
Nanoestruturas/química
Psoríase/tratamento farmacológico
[Mh] Termos MeSH secundário: Administração Tópica
Adulto
Animais
Óleo de Coco
Ciclosporina/farmacologia
Fármacos Dermatológicos/farmacologia
Difusão
Avaliação Pré-Clínica de Medicamentos/métodos
Emulsões/farmacologia
Feminino
Seres Humanos
Myristica fragrans
Nanoestruturas/administração & dosagem
Tamanho da Partícula
Óleos Vegetais/administração & dosagem
Óleos Vegetais/química
Polissacarídeos Bacterianos/química
Ratos
Pele/metabolismo
Solubilidade
Tensoativos/química
Viscosidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dermatologic Agents); 0 (Emulsions); 0 (Plant Oils); 0 (Polysaccharides, Bacterial); 0 (Surface-Active Agents); 83HN0GTJ6D (Cyclosporine); Q9L0O73W7L (Coconut Oil); TTV12P4NEE (xanthan gum)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.2147/IJN.S125302


  6 / 143 MEDLINE  
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[PMID]:27397646
[Au] Autor:Reinholds I; Pugajeva I; Bavrins K; Kuckovska G; Bartkevics V
[Ad] Endereço:a Institute of Food Safety , Animal Health and Environment "BIOR" , Riga , Latvia.
[Ti] Título:Mycotoxins, pesticides and toxic metals in commercial spices and herbs.
[So] Source:Food Addit Contam Part B Surveill;10(1):5-14, 2017 Mar.
[Is] ISSN:1939-3229
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A total of 300 samples representing six condiments (black pepper, basil, oregano, nutmeg, paprika, and thyme) were analysed for 11 mycotoxins, 134 pesticides and 4 heavy metals by ultra-high performance liquid chromatography-tandem quadrupole mass spectrometry and inductively coupled plasma mass spectrometry. Mycotoxins were detected in 4%, 10% and 30% of all nutmeg, basil and thyme samples, respectively. The residues of 24 pesticides were detected in 59% of the analysed condiments. The maximum residue levels of pesticides were exceeded in 10% of oregano and 46% of thyme samples. A risk assessment of heavy metals was performed, indicating daily intake levels far below the tolerable intake levels.
[Mh] Termos MeSH primário: Exposição Ambiental/análise
Contaminação de Alimentos/análise
Metais Pesados/análise
Micotoxinas/análise
Resíduos de Praguicidas/análise
Plantas Comestíveis/química
Especiarias/análise
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Comércio
Dieta
Seres Humanos
Myristica fragrans
Ocimum basilicum
Origanum
Praguicidas
Folhas de Planta/química
Medição de Risco
Sementes/química
Espectrometria de Massas em Tandem
Thymus (Planta)
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Metals, Heavy); 0 (Mycotoxins); 0 (Pesticide Residues); 0 (Pesticides)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170621
[Lr] Data última revisão:
170621
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160712
[St] Status:MEDLINE
[do] DOI:10.1080/19393210.2016.1210244


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[PMID]:27845864
[Au] Autor:Zhang Y; Xie P; Guo X; Kang W
[Ad] Endereço:1 Institute of Chinese Materia Medica, Pharmaceutical College, Henan University , Kaifeng, China .
[Ti] Título:Procoagulant Substance and Mechanism of Myristica fragrans.
[So] Source:J Med Food;19(11):1065-1073, 2016 Nov.
[Is] ISSN:1557-7600
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The effect and mechanisms of Myristica fragrans on blood clotting were evaluated by evaluating blood coagulation time and the fibrinolytic system. The compounds 2 and 5 were isolated from the herbal extract and their activities were assessed for the first time. None of the tested compounds had fibrinolytic activity, but could inhibit the fibrinolytic activity of urokinase. Compound 2 showed the highest inhibitory activity (IC = 1.747 mg·mL ) followed by compounds 4 (IC = 1.818 mg·mL ) and 1 (IC = 2.407 mg·mL ), which were higher than that of the compound in Danshen drug tablets (IC = 6.577 mg·mL ) used in China. Moreover, compounds 1 and 2 showed strong α-glucosidase inhibitory activity in a dose-dependent manner with IC values 21.76 ± 0.59 and 21.31 ± 0.00 µg·mL , respectively. These results demonstrated that the compounds are promising candidates as procoagulant and antidiabetic agents.
[Mh] Termos MeSH primário: Coagulação Sanguínea/efeitos dos fármacos
Medicamentos de Ervas Chinesas/farmacologia
Fibrinolíticos/farmacologia
Myristica fragrans/química
[Mh] Termos MeSH secundário: Animais
Relação Dose-Resposta a Droga
Medicamentos de Ervas Chinesas/química
Medicamentos de Ervas Chinesas/isolamento & purificação
Fibrina/metabolismo
Fibrinolíticos/química
Fibrinolíticos/isolamento & purificação
Inibidores de Glicosídeo Hidrolases/farmacologia
Hipoglicemiantes/farmacologia
Lignanas/farmacologia
Masculino
Modelos Animais
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/farmacologia
Coelhos
Salvia miltiorrhiza/química
Sementes/química
Ativador de Plasminogênio Tipo Uroquinase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Fibrinolytic Agents); 0 (Glycoside Hydrolase Inhibitors); 0 (Hypoglycemic Agents); 0 (Lignans); 0 (Plant Extracts); 79483-68-4 (dan-shen root extract); 9001-31-4 (Fibrin); EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170526
[Lr] Data última revisão:
170526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161116
[St] Status:MEDLINE


  8 / 143 MEDLINE  
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[PMID]:27511913
[Au] Autor:Song JS; Kim EK; Choi YW; Oh WK; Kim YM
[Ad] Endereço:Department of Pharmacy, College of Pharmacy and Institute of Pharmaceutical Science and Technology, Hanyang University, Ansan, Gyeonggi-do 15588, Republic of Korea.
[Ti] Título:Hepatocyte-protective effect of nectandrin B, a nutmeg lignan, against oxidative stress: Role of Nrf2 activation through ERK phosphorylation and AMPK-dependent inhibition of GSK-3ß.
[So] Source:Toxicol Appl Pharmacol;307:138-149, 2016 Sep 15.
[Is] ISSN:1096-0333
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Oxidative stress can contribute to the development and progression of liver diseases, such as drug-induced or alcoholic liver injury, nonalcoholic fatty liver disease, and nonalcoholic steatohepatitis. Nectandrin B is a bioactive lignan isolated from nutmeg extract. To date, little information is available about its pharmacological activities in the liver. This study investigated the hepatocyte-protective effect of nectandrin B against tert-butylhydroperoxide-induced oxidative injury and the underlying molecular mechanism. The cell viability assay revealed that nectandrin B prevents apoptosis stimulated by tert-butylhydroperoxide in both HepG2 cells and primary mouse hepatocytes. Nectandrin B also attenuated ROS production and restored the depleted glutathione level. Real-time PCR and immunoblot analyses showed that the expression of glutamate-cysteine ligase, an enzyme responsible for the glutathione biosynthesis, was induced by nectandrin B, indicating its indirect antioxidative effect. The NF-E2-related factor-2 (Nrf2) regulates gene expression of an array of antioxidant enzymes in hepatocytes. Nectandrin B stimulated Nrf2 activation as evidenced by its enhanced nuclear accumulation and increased antioxidant response element (ARE)-luciferase activity. Intriguingly, the hepatocyte-protective effect of nectandrin B against oxidative damage was completely abrogated by Nrf2 knockdown using Nrf2 specific siRNA. Nectandrin B promoted ERK activation, but inactivated GSK-3ß through the AMPK-mediated inhibitory phosphorylation. The enforced overexpression of dominant-negative mutant of MEK1 or AMPKα, or wild-type GSK-3ß inhibited the increase in the NQO1-ARE-luciferase activity stimulated by nectandrin B, suggesting that both ERK and AMPK-GSK-3ß signalings are involved in the activation of Nrf2/ARE pathway by nectandrin B. Consistent with this, cytoprotection and restoration of glutathione level by nectandrin B was also blocked by the overexpression of dominant-negative MEK1 or wild-type GSK-3ß. Finally, our data demonstrate that nectandrin B has the ability to protect hepatocytes against oxidative injury through the activation of Nrf2/ARE pathway mediated by ERK phosphorylation and AMPK-dependent inactivation of GSK-3ß.
[Mh] Termos MeSH primário: Hepatócitos/efeitos dos fármacos
Lignanas/farmacologia
Fator 2 Relacionado a NF-E2/metabolismo
Substâncias Protetoras/farmacologia
[Mh] Termos MeSH secundário: Proteínas Quinases Ativadas por AMP/genética
Proteínas Quinases Ativadas por AMP/metabolismo
Animais
Apoptose/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Células Cultivadas
Glutationa/metabolismo
Glicogênio Sintase Quinase 3 beta/genética
Glicogênio Sintase Quinase 3 beta/metabolismo
Células Hep G2
Hepatócitos/metabolismo
Seres Humanos
Luciferases/genética
Luciferases/metabolismo
MAP Quinase Quinase 1/genética
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Camundongos Endogâmicos C57BL
Myristica fragrans
Estresse Oxidativo/efeitos dos fármacos
Fosforilação/efeitos dos fármacos
Espécies Reativas de Oxigênio/metabolismo
terc-Butil Hidroperóxido
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Lignans); 0 (NF-E2-Related Factor 2); 0 (Protective Agents); 0 (Reactive Oxygen Species); 0 (nectandrin-B); 955VYL842B (tert-Butylhydroperoxide); EC 1.13.12.- (Luciferases); EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta); EC 2.7.11.31 (AMP-Activated Protein Kinases); EC 2.7.12.2 (MAP Kinase Kinase 1); EC 2.7.12.2 (MAP2K1 protein, human); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160812
[St] Status:MEDLINE


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[PMID]:27430914
[Au] Autor:Kim EY; Choi HJ; Park MJ; Jung YS; Lee SO; Kim KJ; Choi JH; Chung TW; Ha KT
[Ad] Endereço:* Healthy Aging Korean Medical Research Center, School of Korean Medicine, Pusan National University, Yangsan, Gyeongsangnam-do, Republic of Korea.
[Ti] Título:Myristica fragrans Suppresses Tumor Growth and Metabolism by Inhibiting Lactate Dehydrogenase A.
[So] Source:Am J Chin Med;44(5):1063-79, 2016.
[Is] ISSN:0192-415X
[Cp] País de publicação:Singapore
[La] Idioma:eng
[Ab] Resumo:Most cancer cells predominantly produce ATP by maintaining a high rate of lactate fermentation, rather than by maintaining a comparatively low rate of tricarboxylic acid cycle, i.e., Warburg's effect. In the pathway, the pyruvate produced by glycolysis is converted to lactic acid by lactate dehydrogenase (LDH). Here, we demonstrated that water extracts from the seeds of Myristica fragrans Houtt. (MF) inhibit the in vitro enzymatic activity of LDH. MF effectively suppressed cell growth and the overall Warburg effect in HT29 human colon cancer cells. Although the expression of LDH-A was not changed by MF, both lactate production and LDH activity were decreased in MF-treated cells under both normoxic and hypoxic conditions. In addition, intracellular ATP levels were also decreased by MF treatment, and the uptake of glucose was also reduced by MF treatment. Furthermore, the experiment on tumor growth in the in vivo mice model revealed that MF effectively reduced the growth of allotransplanted Lewis lung carcinoma cells. Taken together, these results suggest that MF effectively inhibits cancer growth and metabolism by inhibiting the activity of LDH, a major enzyme responsible for regulating cancer metabolism. These results implicate MF as a potential candidate for development into a novel drug against cancer through inhibition of LDH activity.
[Mh] Termos MeSH primário: Proliferação Celular/efeitos dos fármacos
L-Lactato Desidrogenase/metabolismo
Myristica fragrans/química
Neoplasias/enzimologia
Neoplasias/metabolismo
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Linhagem Celular Tumoral
Glucose/metabolismo
Células HT29
Seres Humanos
Isoenzimas/genética
Isoenzimas/metabolismo
L-Lactato Desidrogenase/genética
Ácido Láctico/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Neoplasias/tratamento farmacológico
Neoplasias/fisiopatologia
Extratos Vegetais/química
Sementes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Isoenzymes); 0 (Plant Extracts); 33X04XA5AT (Lactic Acid); EC 1.1.1.27 (L-Lactate Dehydrogenase); EC 1.1.1.27.- (lactate dehydrogenase 5); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170117
[Lr] Data última revisão:
170117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160720
[St] Status:MEDLINE
[do] DOI:10.1142/S0192415X16500592


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[PMID]:27419473
[Au] Autor:Morikawa T; Hachiman I; Matsuo K; Nishida E; Ninomiya K; Hayakawa T; Yoshie O; Muraoka O; Nakayama T
[Ti] Título:Neolignans from the Arils of Myristica fragrans as Potent Antagonists of CC Chemokine Receptor 3.
[So] Source:J Nat Prod;79(8):2005-13, 2016 Aug 26.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:CC chemokine receptor 3 (CCR3) is expressed selectively in eosinophils, basophils, and some Th2 cells and plays a major role in allergic diseases. A methanol extract from the arils of Myristica fragrans inhibited CC chemokine ligand 11-induced chemotaxis in CCR3-expressing L1.2 cells at 100 µg/mL. From this extract, eight new neolignans, maceneolignans A-H (1-8), were isolated, and their stereostructures were elucidated from their spectroscopic values and chemical properties. Of those constituents, compounds 1, 4, 6, and 8 and (+)-erythro-(7S,8R)-Δ(8')-7-hydroxy-3,4-methylenedioxy-3',5'-dimethoxy-8-O-4'-neolignan (11), (-)-(8R)-Δ(8')-3,4-methylenedioxy-3',5'-dimethoxy-8-O-4'-neolignan (17), (+)-licarin A (20), nectandrin B (25), verrucosin (26), and myristicin (27) inhibited CCR3-mediated chemotaxis at a concentration of 1 µM. Among them, 1 (EC50 1.6 µM), 6 (1.5 µM), and 8 (1.4 µM) showed relatively strong activities, which were comparable to that of a synthetic CCR3 selective antagonist, SB328437 (0.78 µM).
[Mh] Termos MeSH primário: Lignanas/isolamento & purificação
Lignanas/farmacologia
Myristica fragrans/química
Receptores CCR3/antagonistas & inibidores
[Mh] Termos MeSH secundário: Quimiotaxia/efeitos dos fármacos
Eosinófilos/metabolismo
Furanos/farmacologia
Seres Humanos
Lignanas/química
Estrutura Molecular
Estereoisomerismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Furans); 0 (Lignans); 0 (Receptors, CCR3); 0 (nectandrin-B); 0 (verrucosin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160716
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b00262



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