Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.773.522 [Categoria DeCS]
Referências encontradas : 22 [refinar]
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  1 / 22 MEDLINE  
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[PMID]:28627169
[Au] Autor:Valero-Cases E; Roy NC; Frutos MJ; Anderson RC
[Ad] Endereço:Research Group on Food Quality and Safety. Agro-Food Technology Department, Miguel Hernández University , Ctra. Beniel, Km. 3.2, 03312 Orihuela, Alicante, Spain.
[Ti] Título:Influence of the Fruit Juice Carriers on the Ability of Lactobacillus plantarum DSM20205 to Improve in Vitro Intestinal Barrier Integrity and Its Probiotic Properties.
[So] Source:J Agric Food Chem;65(28):5632-5638, 2017 Jul 19.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This study investigates the influence of tomato and feijoa juices as fermentable carriers of Lactobacillus plantarum (LP DSM20205) on the ability of the bacterium to improve intestinal barrier function using the trans-epithelial electrical resistance (TEER) assay in an apical anaerobic model. The survival of LP DSM20205 in different fruit juices during in vitro digestion, its adhesion capacity, and potential cytotoxic effect on Caco-2 cells were also studied. The results showed that carrier fruit juices have a significant influence on LP DSM20205 growth, survival during in vitro digestion, adhesion capacity, and TEER. All fermented samples were not cytotoxic to Caco-2 cells. The fermented tomato juice showed the largest improvement to intestinal barrier integrity. The digested fermented juices did not increase TEER, although the LP DSM20205 in these samples adhered well. Therefore, LP DSM20205 has the potential to be used as a probiotic in the production of fermented tomato and feijoa juices.
[Mh] Termos MeSH primário: Sucos de Frutas e Vegetais/microbiologia
Intestinos/microbiologia
Lactobacillus plantarum/metabolismo
Probióticos/metabolismo
[Mh] Termos MeSH secundário: Células CACO-2
Feijoa/microbiologia
Fermentação
Seres Humanos
Intestinos/metabolismo
Lactobacillus plantarum/crescimento & desenvolvimento
Lycopersicon esculentum/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170728
[Lr] Data última revisão:
170728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01551


  2 / 22 MEDLINE  
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[PMID]:28334322
[Au] Autor:Rosa JMD; Arioli CJ; Santos JPD; Menezes-Netto AC; Botton M
[Ad] Endereço:Universidade do Estado de Santa Catarina, Laboratório de Entomologia, Lages, SC 88520-000, Brazil ( joatanmachado@gmail.com ).
[Ti] Título:Evaluation of Food Lures for Capture and Monitoring of Anastrepha fraterculus (Diptera: Tephritidae) on Temperate Fruit Trees.
[So] Source:J Econ Entomol;110(3):995-1001, 2017 Jun 01.
[Is] ISSN:1938-291X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae) is the main pest of fruit trees grown in temperate climates in the southern region of Brazil. The objective of this work was to evaluate the efficiency of the major commercial food lures used in Brazil for trapping and monitoring of A. fraterculus in plum, pear, and feijoa orchards. The assessed lures were hydrolyzed proteins of animal origin (CeraTrap) and plant origin (BioAnastrepha), torula yeast + borax (Torula), and grape juice. Response variables included the rate of adult capture (flies per trap per day, FTD) and the percentage of females captured. We also evaluated the number of times the weekly capture rate exceeded the traditional threshold of 0.5 FTD for each lure. Traps baited with grape juice, currently used for monitoring A. fraterculus in Southern Brazil, captured fewer adults and a lower percentage of females compared with the other lures. CeraTrap trapped a greater number of A. fraterculus adults and, in some cases, a lower percentage of females compared with the other lures in pears. Traps baited with CeraTrap had greater capture rates (FTD), particularly during the stages of fruit maturation and harvest, and even in years with low population density of A. fraterculus, thus demonstrating greater sensitivity in the detection of this pest. These results show that, in order to detect and monitor the presence of A. fraterculus in plum, feijoa, and pear crops, protein-based lures are superior to grape juice, especially the animal protein CeraTrap.
[Mh] Termos MeSH primário: Quimiotaxia
Controle de Insetos/métodos
Feromônios/farmacologia
Tephritidae/fisiologia
[Mh] Termos MeSH secundário: Animais
Brasil
Feijoa/crescimento & desenvolvimento
Feminino
Masculino
Odorantes/análise
Prunus domestica/crescimento & desenvolvimento
Pyrus/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Pheromones)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1093/jee/tox084


  3 / 22 MEDLINE  
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[PMID]:28185042
[Au] Autor:Machado LO; Vieira LD; Stefenon VM; Oliveira Pedrosa F; Souza EM; Guerra MP; Nodari RO
[Ad] Endereço:Laboratório de Fisiologia do Desenvolvimento e Genética Vegetal, Programa de Pós-graduação em Recursos Genéticos Vegetais, Universidade Federal de Santa Catarina, Rodovia Admar Gonzaga, 1346, 88040-900, Florianópolis, Santa Catarina, Brazil.
[Ti] Título:Phylogenomic relationship of feijoa (Acca sellowiana (O.Berg) Burret) with other Myrtaceae based on complete chloroplast genome sequences.
[So] Source:Genetica;145(2):163-174, 2017 Apr.
[Is] ISSN:1573-6857
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Given their distribution, importance, and richness, Myrtaceae species comprise a model system for studying the evolution of tropical plant diversity. In addition, chloroplast (cp) genome sequencing is an efficient tool for phylogenetic relationship studies. Feijoa [Acca sellowiana (O. Berg) Burret; CN: pineapple-guava] is a Myrtaceae species that occurs naturally in southern Brazil and northern Uruguay. Feijoa is known for its exquisite perfume and flavorful fruits, pharmacological properties, ornamental value and increasing economic relevance. In the present work, we reported the complete cp genome of feijoa. The feijoa cp genome is a circular molecule of 159,370 bp with a quadripartite structure containing two single copy regions, a Large Single Copy region (LSC 88,028 bp) and a Small Single Copy region (SSC 18,598 bp) separated by Inverted Repeat regions (IRs 26,372 bp). The genome structure, gene order, GC content and codon usage are similar to those of typical angiosperm cp genomes. When compared to other cp genome sequences of Myrtaceae, feijoa showed closest relationship with pitanga (Eugenia uniflora L.). Furthermore, a comparison of pitanga synonymous (Ks) and nonsynonymous (Ka) substitution rates revealed extremely low values. Maximum Likelihood and Bayesian Inference analyses produced phylogenomic trees identical in topology. These trees supported monophyly of three Myrtoideae clades.
[Mh] Termos MeSH primário: DNA Circular/genética
Feijoa/genética
Genoma de Cloroplastos/genética
Myrtaceae/genética
[Mh] Termos MeSH secundário: Composição de Bases/genética
Teorema de Bayes
Brasil
Códon/genética
DNA Circular/química
Feijoa/classificação
Ordem dos Genes
Genes de Cloroplastos/genética
Cadeias de Markov
Método de Monte Carlo
Mutação
Myrtaceae/classificação
Filogenia
Polimorfismo de Nucleotídeo Único
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Codon); 0 (DNA, Circular)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1007/s10709-017-9954-1


  4 / 22 MEDLINE  
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[PMID]:27166598
[Au] Autor:Turco F; Palumbo I; Andreozzi P; Sarnelli G; De Ruberto F; Esposito G; Basile A; Cuomo R
[Ad] Endereço:Department of Clinical and Experimental Medicine, Federico II University of Naples, Naples, Italy.
[Ti] Título:Acetonic Extract from the Feijoa sellowiana Berg. Fruit Exerts Antioxidant Properties and Modulates Disaccharidases Activities in Human Intestinal Epithelial Cells.
[So] Source:Phytother Res;30(8):1308-15, 2016 Aug.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Feijoa sellowiana fruit has been shown to possess various biological activities, such as anti-bacterial and anti-cancer properties, in a variety of cellular models, but its activity on human intestinal epithelial cells has never been tested. The purpose of this study was to investigate the effects of the acetonic extract of F. sellowiana fruits on the viability, membrane peroxidation, disaccharidases activities and proliferation of in vitro models of human intestinal epithelial cells. To obtain this goal, Caco-2 and HT-29 cells were exposed to the acetonic extract for 24 h. Cell proliferation, viability, lactase and sucrase-isomaltase activity and H2 O2 -induced membrane lipid peroxidation were tested. We found that, compared to control conditions, the acetonic extract significantly increased lactase and sucrase-isomaltase activity in Caco-2, but not HT-29, cells, decreased proliferation, had no effects on viability and restored lipid peroxidation in both cell models. This study suggests that the acetonic extract improves lactase and sucrase-isomaltase activity, inhibits cell proliferation, have no cytotoxic effects and prevent lipid peroxidation of intestinal epithelial cells. These effects may be exploited in case of disaccharidases deficit and also as an adjuvant treatment of diseases related to oxidative stress. Copyright © 2016 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Dissacaridases/química
Células Epiteliais/metabolismo
Feijoa/química
Frutas/química
Intestinos/metabolismo
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Antioxidantes
Seres Humanos
Extratos Vegetais/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts); EC 3.2.1.- (Disaccharidases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160512
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5629


  5 / 22 MEDLINE  
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[PMID]:26444875
[Au] Autor:Duong T; Balaban M; Perera C; Bi X
[Ad] Endereço:School of Chemical Sciences, The Univ. of Auckland, Auckland, New Zealand.
[Ti] Título:Microbial and Sensory Effects of Combined High Hydrostatic Pressure and Dense Phase Carbon Dioxide Process on Feijoa Puree.
[So] Source:J Food Sci;80(11):E2478-85, 2015 Nov.
[Is] ISSN:1750-3841
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:UNLABELLED: High hydrostatic pressure (HHP) is used for microbial inactivation in foods. Addition of carbon dioxide (CO2) to HHP can improve microbial and enzyme inactivation. This study investigated microbial effects of combined HHP and CO2 on Escherichia coli, Bacillus subtilis, and Saccharomyces cerevisiae, and evaluated sensory attributes of treated feijoa fruit puree (pH 3.2). Microorganisms in their growth media and feijoa puree were treated with HHP alone (HHP), or saturated with CO2 at 1 atm (HHPcarb), or 0.4%w/w of CO2 was injected into the package (HHPcarb+CO2). Microbial samples were processed at 200 to 400 MPa, 25 °C, 2 to 6 min. Feijoa samples were processed at 600 MPa, 20 °C, 5 min, then served with and without added sucrose (10%w/w). Treated samples were analyzed for microbial viability and sensory evaluation. Addition of CO2 enhanced microbial inactivation of HHP from 1.7-log to 4.3-log reduction in E. coli at 400 MPa, 4 min, and reduction of >6.5 logs in B. subtilis (vegetative cells) starting at 200 MPa, 2 min. For yeast, HHPcarb+CO2 increased the inactivation of HHP from 4.7-log to 6.2-log reduction at 250 MPa, 4 min. The synergistic effect of CO2 with HHP increased with increasing time and pressure. HHPcarb+CO2 treatment did not alter the appearance and color, while affecting the texture and flavor of unsweetened feijoa samples. There were no differences in sensory attributes and preferences between HHPcarb+CO2 and fresh sweetened products. Addition of CO2 in HHP treatment can reduce process pressure and time, and better preserve product quality. PRACTICAL APPLICATION: A higher microbial inactivation of Escherichia coli, Bacillus subtilis and Saccharomyces cerevisiae by combining dense phase carbon dioxide and high hydrostatic pressure was observed. For sweetened products there were no significant differences in sensory attributes and preferences between samples treated by the combined method and the fresh samples. In conclusion, addition of CO2 in HHP treatment of juices could reduce process severity and improve product quality.
[Mh] Termos MeSH primário: Bactérias/efeitos dos fármacos
Dióxido de Carbono/farmacologia
Feijoa
Conservação de Alimentos/métodos
Frutas/microbiologia
Pressão Hidrostática
Paladar
[Mh] Termos MeSH secundário: Contagem de Colônia Microbiana
Manipulação de Alimentos/métodos
Microbiologia de Alimentos
Seres Humanos
Viabilidade Microbiana/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
142M471B3J (Carbon Dioxide)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:151109
[Lr] Data última revisão:
151109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151008
[St] Status:MEDLINE
[do] DOI:10.1111/1750-3841.13083


  6 / 22 MEDLINE  
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[PMID]:26214790
[Au] Autor:Mahmoudi M; Ebrahimzadeh MA; Abdi M; Arimi Y; Fathi H
[Ad] Endereço:Department of Pharmacology, School of Medicine, Pharmaceutical Sciences Research Center; Mazandaran University of Medical Sciences, Sari, Iran. zadeh20@gmail.com.
[Ti] Título:Antidepressant activities of Feijoa sellowiana fruit.
[So] Source:Eur Rev Med Pharmacol Sci;19(13):2510-3, 2015 Jul.
[Is] ISSN:2284-0729
[Cp] País de publicação:Italy
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: Many pharmacological activities have been reported for Feijoa sellowiana. The aim of present study was to investigate antidepressant activities of its leaf and fruit extracts. MATERIALS AND METHODS: Antidepressant activities of methanolic extracts were evaluated by modified forced swimming test (FST) and tail suspension tests (TST) in male Swiss albino mice. RESULTS: Extracts showed signicant antidepressant activity in both models. They shortened remarkably the immobility period in both FST and TST and exhibited a dose dependent activity (p < 0.001). Leaf extract showed better activity than fruit extract. At 800 mg kg-1, it showed far better activity than imipramine in FST (p < 0.001). Both extracts showed significantly better activity than imipramine in increasing climbing time (p < 0.001). They showed significant activity in increasing in swimming time as compared to the control group (p < 0.001). CONCLUSIONS: Our studies indicate that Feijoa showed significant antidepressant activity. It produced dose dependent effect on both models. It seems this effect is mainly mediated by inhibition of reuptake of catecholamines. These results introduced these plants as easily accessible source of natural antidepressant.
[Mh] Termos MeSH primário: Antidepressivos/farmacologia
Feijoa
Frutas
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Animais
Antidepressivos/isolamento & purificação
Relação Dose-Resposta a Droga
Elevação dos Membros Posteriores/métodos
Elevação dos Membros Posteriores/psicologia
Masculino
Camundongos
Atividade Motora/efeitos dos fármacos
Extratos Vegetais/isolamento & purificação
Folhas de Planta
Natação/psicologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antidepressive Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150728
[Lr] Data última revisão:
150728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150728
[St] Status:MEDLINE


  7 / 22 MEDLINE  
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[PMID]:26110654
[Au] Autor:Ahmed Nasef N; Mehta S; Powell P; Marlow G; Wileman T; Ferguson LR
[Ad] Endereço:Faculty of Medical & Health Sciences, The University of Auckland, Auckland, New Zealand.
[Ti] Título:Extracts of Feijoa Inhibit Toll-Like Receptor 2 Signaling and Activate Autophagy Implicating a Role in Dietary Control of IBD.
[So] Source:PLoS One;10(6):e0130910, 2015.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Inflammatory bowel disease (IBD) is a heterogeneous chronic inflammatory disease affecting the gut with limited treatment success for its sufferers. This suggests the need for better understanding of the different subtypes of the disease as well as nutritional interventions to compliment current treatments. In this study we assess the ability of a hydrophilic feijoa fraction (F3) to modulate autophagy a process known to regulate inflammation, via TLR2 using IBD cell lines. METHOD: Mouse embryonic fibroblasts (MEF) deleted for ATG5, and two intestinal epithelial cells HCT15 and HCT116, were used to test the anti-inflammatory effect of F3 after stimulating the cells with a TLR2 specific ligand PAM3CSK4. RESULTS: F3 was able to reduce TLR2 specific inflammation and stimulate autophagy in MEFs and HCT15 cells but not in HCT116 cells. The anti-inflammatory effect was reduced in the MEF cells deleted for ATG5. In addition, the activation of autophagy by F3 was enhanced by PAM3CSK4. CONCLUSION: F3 of feijoa can interact with cells via a TLR2 specific mechanism and reduce Nuclear factor kappa B (NF-κB) activation in part due to stimulation of autophagy. These results suggest that there is potential benefit in using feijoa extracts as part of dietary interventions to manage IBD in patients.
[Mh] Termos MeSH primário: Autofagia/efeitos dos fármacos
Feijoa
Doenças Inflamatórias Intestinais/metabolismo
Extratos Vegetais/farmacologia
Transdução de Sinais/efeitos dos fármacos
Receptor 2 Toll-Like/metabolismo
[Mh] Termos MeSH secundário: Animais
Linhagem Celular
Células Epiteliais/efeitos dos fármacos
Células Epiteliais/metabolismo
Fibroblastos/efeitos dos fármacos
Fibroblastos/metabolismo
Mucosa Intestinal/metabolismo
Camundongos
NF-kappa B/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (NF-kappa B); 0 (Plant Extracts); 0 (Toll-Like Receptor 2)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150702
[Lr] Data última revisão:
150702
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150626
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0130910


  8 / 22 MEDLINE  
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[PMID]:24433073
[Au] Autor:Monforte MT; Fimiani V; Lanuzza F; Naccari C; Restuccia S; Galati EM
[Ad] Endereço:1 Department of Pharmaceutical Sciences and Health Products (SCIFAR), University of Messina , Messina, Italy .
[Ti] Título:Feijoa sellowiana Berg fruit juice: anti-inflammatory effect and activity on superoxide anion generation.
[So] Source:J Med Food;17(4):455-61, 2014 Apr.
[Is] ISSN:1557-7600
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Feijoa sellowiana Berg var. coolidge fruit juice was studied in vivo for the anti-inflammatory activity by carrageenin-induced paw edema test and in vitro for the effects on superoxide anion release from neutrophils in human whole blood. The fruit juice was analyzed by the high-performance liquid chromatography method, and quercetin, ellagic acid, catechin, rutin, eriodictyol, gallic acid, pyrocatechol, syringic acid, and eriocitrin were identified. The results showed a significant anti-inflammatory activity of F. sellowiana fruit juice, sustained also by an effective antioxidant activity observed in preliminary studies on 1,1-diphenyl-2-picrylhydrazyl (DPPH) test. In particular, the anti-inflammatory activity edema inhibition is significant since the first hour (44.11%) and persists until the fifth hour (44.12%) of the treatment. The effect on superoxide anion release was studied in human whole blood, in the presence of activators affecting neutrophils by different mechanisms. The juice showed an inhibiting response on neutrophils basal activity in all experimental conditions. In stimulated neutrophils, the higher inhibition of superoxide anion generation was observed at concentration of 10(-4) and 10(-2) mg/mL in whole blood stimulate with phorbol-myristate-13-acetate (PMA; 20% and 40%) and with N-formyl-methionyl-leucyl-phenylalanine (FMLP; 15% and 48%). The significant reduction of edema and the inhibition of O2(-) production, occurring mainly through interaction with protein-kinase C pathway, confirm the anti-inflammatory effect of F. sellowiana fruit juice.
[Mh] Termos MeSH primário: Ânions/metabolismo
Anti-Inflamatórios/administração & dosagem
Antioxidantes/administração & dosagem
Bebidas/análise
Edema/tratamento farmacológico
Feijoa/química
Extratos Vegetais/administração & dosagem
Superóxidos/metabolismo
[Mh] Termos MeSH secundário: Animais
Ânions/análise
Anti-Inflamatórios/análise
Anti-Inflamatórios/isolamento & purificação
Antioxidantes/análise
Antioxidantes/isolamento & purificação
Edema/imunologia
Edema/metabolismo
Frutas/química
Seres Humanos
Masculino
Neutrófilos/química
Neutrófilos/efeitos dos fármacos
Neutrófilos/metabolismo
Extratos Vegetais/análise
Extratos Vegetais/isolamento & purificação
Ratos
Ratos Wistar
Superóxidos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anions); 0 (Anti-Inflammatory Agents); 0 (Antioxidants); 0 (Plant Extracts); 11062-77-4 (Superoxides)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:140411
[Lr] Data última revisão:
140411
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140118
[St] Status:MEDLINE
[do] DOI:10.1089/jmf.2012.0262


  9 / 22 MEDLINE  
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[PMID]:23849129
[Au] Autor:Fraga HP; Agapito-Tenfen SZ; Caprestano CA; Nodari RO; Guerra MP
[Ad] Endereço:Graduate Program in Plant Genetic Resources, Plant Developmental Physiology and Genetics Laboratory, Federal University of Santa Catarina, Rodovia Admar Gonzaga, 1346, ZC: 88040-900 Florianópolis, Santa Catarina, Brazil.
[Ti] Título:Comparative proteomic analysis of off-type and normal phenotype somatic plantlets derived from somatic embryos of Feijoa (Acca sellowiana (O. Berg) Burret).
[So] Source:Plant Sci;210:224-31, 2013 Sep.
[Is] ISSN:1873-2259
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:Morphological disorders in a relevant portion of emerged somatic embryos have been a limiting factor in the true-to-type plantlet formation in Acca sellowiana. In this sense, the present study undertook a comparison between normal phenotype and off-type somatic plantlets protein profiles by means of the 2-D DIGE proteomics approach. Off-type and normal phenotype somatic plantlets obtained at 10 and 20 days conversion were evaluated. Results indicated 12 exclusive spots between normal and off-type plantlets at 10 days conversion, and 17 exclusive spots at 20 days conversion. Also at 20 days conversion, 4 spots were differentially expressed, up- or down-regulated. Two proteins related to carbohydrate metabolism were only expressed in off-types at 10 days conversion, suggesting a more active respiratory pathway. A vicilin-like storage protein was only found in off-types at 20 days conversion, indicating that plantlets may present an abnormality in the mobilization of storage compounds, causing reduced vigor in the development of derived plantlets. The presence of heat shock proteins were only observed during formation of normal phenotype somatic plantlets, indicating that these proteins may be involved in normal morphogenesis of plantlets formed. These new findings shed light on possible genetic or epigenetic mechanisms governing A. sellowiana morphogenesis.
[Mh] Termos MeSH primário: Feijoa/metabolismo
Proteínas de Plantas/análise
Proteômica
[Mh] Termos MeSH secundário: Feijoa/anatomia & histologia
Feijoa/genética
Feijoa/crescimento & desenvolvimento
Proteínas de Choque Térmico/análise
Proteínas de Choque Térmico/metabolismo
Fenótipo
Proteínas de Plantas/metabolismo
Técnicas de Embriogênese Somática de Plantas
Proteínas de Armazenamento de Sementes/análise
Proteínas de Armazenamento de Sementes/metabolismo
Espectrometria de Massas em Tandem
Eletroforese em Gel Diferencial Bidimensional
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Heat-Shock Proteins); 0 (Plant Proteins); 0 (Seed Storage Proteins); 0 (vicilin-like protein, plant)
[Em] Mês de entrada:1401
[Cu] Atualização por classe:130715
[Lr] Data última revisão:
130715
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130716
[St] Status:MEDLINE


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[PMID]:23179689
[Au] Autor:Guerra MP; Cangahuala-Inocente GC; Vesco LL; Pescador R; Caprestano CA
[Ad] Endereço:Laboratory of Plant Developmental Physiology and Genetics, Graduate Program in Plant Genetic Resources, Federal University of Santa Catarina, Florianópolis, Brazil. mpguerra@cca.ufsc.br
[Ti] Título:Micropropagation systems of Feijoa (Acca sellowiana (O. Berg) Burret).
[So] Source:Methods Mol Biol;11013:45-62, 2013.
[Is] ISSN:1940-6029
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Acca sellowiana (O. Berg) Burret sin. Feijoa sellowiana (Myrtaceae) is a semiwoody fruit species native to South Brazil, Uruguay, and Argentina; edible fruits are tasty. The naturally occurring populations in Santa Catarina State show high variability in fruit size, color, and other features. A breeding program launched in 1990 resulted in the release of four Brazilian commercial varieties. The conventional clonal propagation methods of this species, such as cutting and grafting, have shown low efficiency. Therefore, tissue culture techniques were developed for mass propagation. This chapter describes several protocols based on organogenesis and somatic embryogenesis. Additional techniques including synthetic seed technology and temporary immersion system are also described.
[Mh] Termos MeSH primário: Técnicas de Cultura/métodos
Feijoa/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Aclimatação
Feijoa/genética
Feijoa/fisiologia
Flores/crescimento & desenvolvimento
Flores/fisiologia
Genótipo
Imersão
Meristema/crescimento & desenvolvimento
Meristema/fisiologia
Organogênese
Técnicas de Embriogênese Somática de Plantas
Regeneração
Plântulas/crescimento & desenvolvimento
Plântulas/fisiologia
Sementes/crescimento & desenvolvimento
Sementes/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1305
[Cu] Atualização por classe:151119
[Lr] Data última revisão:
151119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:121127
[St] Status:MEDLINE
[do] DOI:10.1007/978-1-62703-074-8_4



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