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[PMID]:28749029
[Au] Autor:Shepherd M; Savins D; Dowell A; Morrow S; Allen G; Southwell I
[Ad] Endereço:Southern Cross Plant Science, Southern Cross University, Lismore, NSW, 2480, Australia.
[Ti] Título:Ecotype Variation of Methyl Eugenol Content in Tea Tree (Melaleuca alternifolia and Melaleuca linariifolia).
[So] Source:Chem Biodivers;14(11), 2017 Nov.
[Is] ISSN:1612-1880
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Methyl eugenol is a natural phenylpropanoid compound found in a wide range of plants used for food, flavouring, cosmetics, and health-care. As a suspected rodent carcinogen, methyl eugenol may also be harmful to humans when present in significant concentrations. Consequently, its level has been restricted in some foodstuffs and cosmetics for some markets. In order to assess the potential to breed uniformly low methyl eugenol cultivars for an essential oil crop, tea tree, the source of 'Oil of Melaleuca, terpinene-4-ol type', we examine levels in individual trees (n = 30) from two geographic regions and six terpene chemotypes. Overall, methyl eugenol levels were low in this species (Mean [SD] 354 [239] ppm, n = 30), much lower than levels predicted to be of toxicological concern. Within each chemotype, there was a lack of evidence for correlations between terpenoid constituents and methyl eugenol levels. Further support for the independence of methyl eugenol and terpene biosynthesis was evident from similar mean levels in selected (Mean [SD] 586 [339] ppm, n = 12) and undomesticated Melaleuca alternifolia trees (Mean [SD] 480 [299] ppm, n = 5) with terpinen-4-ol type oils. By contrast, methyl eugenol level varied by geographic origin and chemotype. Trees from the upland region, where there is a prevalence of terpinolene type trees, had lower average methyl eugenol levels than trees from the coastal region, where there is a prevalence of terpinen-4-ol and 1,8-cineole type trees.
[Mh] Termos MeSH primário: Eugenol/análogos & derivados
Cromatografia Gasosa-Espectrometria de Massas
Melaleuca/química
[Mh] Termos MeSH secundário: Calibragem
Cromatografia Gasosa
Ecótipo
Eugenol/análise
Eugenol/química
Eugenol/normas
Cromatografia Gasosa-Espectrometria de Massas/normas
Limite de Detecção
Melaleuca/metabolismo
Óleos Voláteis/química
Terpenos/análise
Terpenos/química
Terpenos/normas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oils, Volatile); 0 (Terpenes); 29T9VA6R7M (methyleugenol); 3T8H1794QW (Eugenol)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180112
[Lr] Data última revisão:
180112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1002/cbdv.201700278


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[PMID]:28625731
[Au] Autor:Li C; Liu H; Zhao L; Zhang W; Qiu S; Yang X; Tan H
[Ad] Endereço:Program for Natural Product Chemical Biology, Key Laboratory Plant Resources Conservation and Sustainable Utilization, Guangdong Provincial Key Laboratory of Applied Botany, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou 510650, China; Key Laboratory of Natural Pesticide and Ch
[Ti] Título:Antibacterial neolignans from the leaves of Melaleuca bracteata.
[So] Source:Fitoterapia;120:171-176, 2017 Jul.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Phytochemical study on the leaves of Melaleuca bracteata resulted in the isolation of ten compounds including three new neolignans, named melaleucins A-C (1-3). Among them, melaleucin B shares a rarely occurring nor-neolignan skeleton, and both melaleucins B and C bear a novel aldehyde moiety, which might also be response for the delicate fragrance of M. bracteata. Their structures were extensively assigned by spectral data interpretation and biomimetic total synthesis. Moreover, their biosynthetic pathway with oxidative radical coupling and Michael addition as critical reactions was also confirmed. The antimicrobial activity evaluation revealed that melaleucin A exhibited considerable antimicrobial activity towards methicillin-resistant Staphylococcus aureus.
[Mh] Termos MeSH primário: Antibacterianos/química
Lignanas/química
Melaleuca/química
[Mh] Termos MeSH secundário: Antibacterianos/isolamento & purificação
Lignanas/isolamento & purificação
Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos
Estrutura Molecular
Folhas de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Lignans)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170731
[Lr] Data última revisão:
170731
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE


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[PMID]:28480411
[Au] Autor:Shah G; Baghel US
[Ad] Endereço:Research Scholar, IKG Punjab Technical University, Kapurthala, Punjab, India.
[Ti] Título:PHARMACOGNOSTIC STANDARDIZATION OF THE LEAF OF (MAIDEN & BETCHE) CHEEL.
[So] Source:Afr J Tradit Complement Altern Med;14(3):1-11, 2017.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: (Myrtaceae) is a well-known, commonly used, tall shrub plant in Ayurvedic medicine. Traditionally, it is used for its antimicrobial potential to treat cutaneous infections. No attempts have been made regarding pharmacognostic investigation of the plant till date. So, the present study was aimed to establish standards with the help of different pharmacognostic parameters. METHODS: Various pharmacognostic parameters (morphological, microscopic, physicochemical evaluations and preliminary phytochemical screening) were studied along with fluorescent and thin layer chromatographic analysis of the extract. RESULT: Morphologically is a shrub having height of 7 m with layered and papery bark. Leaves have an arranged pattern, petiole is 1 mm in length; linear-acute with dimensions of 10-35 mm x 1 mm. Organoleptic features shows that leaves have characteristic odour and astringent taste. The transverse section of the leaf reveals the existence of epidermal layers, mesophyll tissues, vascular bundles and secretory cavities. The stomata are anomocytic and leaf constants such as stomatal number is 180-200-225, stomatal index is 3.8-4.4-5.9, vein islet number is 18.68 (average), veinlet termination number 20.3 (average) and palisade ratio is 5.5-6.4-6.9. The results of phytochemical screening showed the occurrence of different phytoconstituents (flavonoids, phenolic tannins, phytosterol and terpenoids). CONCLUSION: The present study evaluated various pharmacognostic parameters which will help in quality control (standardization) of leaves in crude form, in herbal formulation and also aid in the preparation of an herbal monograph for the species.
[Mh] Termos MeSH primário: Anti-Infecciosos/análise
Melaleuca/química
Compostos Fitoquímicos/análise
Extratos Vegetais/química
Folhas de Planta/química
[Mh] Termos MeSH secundário: Anti-Infecciosos/farmacologia
Flavonoides/análise
Medicina Ayurvédica
Melaleuca/anatomia & histologia
Compostos Fitoquímicos/farmacologia
Fitosteróis/análise
Extratos Vegetais/farmacologia
Folhas de Planta/anatomia & histologia
Taninos/análise
Terpenos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Flavonoids); 0 (Phytochemicals); 0 (Phytosterols); 0 (Plant Extracts); 0 (Tannins); 0 (Terpenes)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170815
[Lr] Data última revisão:
170815
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170509
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v14i3.1


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[PMID]:28124137
[Au] Autor:Hadas E; Derda M; Cholewinski M
[Ad] Endereço:Department of Biology and Medical Parasitology, Poznan University of Medical Sciences, 10 Fredry Street, 61-701, Poznan, Poland.
[Ti] Título:Evaluation of the effectiveness of tea tree oil in treatment of Acanthamoeba infection.
[So] Source:Parasitol Res;116(3):997-1001, 2017 Mar.
[Is] ISSN:1432-1955
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Eye diseases caused by amoebae from the genus Acanthamoeba are usually chronic and severe, and their treatment is prolonged and not very effective. The difficulties associated with therapy have led to attempts at finding alternative treatment methods. Particularly popular is searching for cures among drugs made of plants. However, no substances with total efficacy in treating Acanthamoeba keratitis have been identified.Results of our semi in vivo studies of tea tree oil simulating eyeball infection demonstrated 100% effectiveness in the case of both trophozoites and cysts of amoebae from the genus Acanthamoeba. The action of tea tree oil indicates that this is the first substance with a potential ability to quickly and effectively remove the amoebae from the eye. Tea tree oil has the ability to penetrate tissues, which allows it to destroy amoebae in both the shallow and deep layers of the cornea. The present research into the use of tea tree oil in the therapy of Acanthamoeba infection is the first study of this type in parasitology. It offers tremendous potential for effective treatment of Acanthamoeba keratitis and other diseases caused by these protozoa.
[Mh] Termos MeSH primário: Ceratite por Acanthamoeba/tratamento farmacológico
Acanthamoeba/efeitos dos fármacos
Melaleuca/química
Óleo de Melaleuca/farmacologia
[Mh] Termos MeSH secundário: Acanthamoeba/fisiologia
Ceratite por Acanthamoeba/parasitologia
Animais
Córnea/parasitologia
Avaliação Pré-Clínica de Medicamentos
Seres Humanos
Camundongos
Camundongos Endogâmicos BALB C
Óleos Vegetais/farmacologia
Resultado do Tratamento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Oils); 68647-73-4 (Tea Tree Oil)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170628
[Lr] Data última revisão:
170628
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE
[do] DOI:10.1007/s00436-017-5377-2


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[PMID]:28089949
[Au] Autor:Souza ME; Lopes LQ; Bonez PC; Gündel A; Martinez DS; Sagrillo MR; Giongo JL; Vaucher RA; Raffin RP; Boligon AA; Santos RC
[Ad] Endereço:Laboratory of Microbiological Research, Centro Universitário Franciscano, Santa Maria, Brazil; Laboratory of Nanotechnology, Post Graduate Program of Nanosciences, Centro Universitário Franciscano, Santa Maria, Brazil.
[Ti] Título:Melaleuca alternifolia nanoparticles against Candida species biofilms.
[So] Source:Microb Pathog;104:125-132, 2017 Mar.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Candida infection is an important cause of morbidity and mortality on immunosuppressed patients. This growing trend has been associated with resistance to the antimicrobial therapy and the ability of microorganism to form biofilms. TTO oil is used as antimicrobial which shows antibiofilm activity against Candida species. However, it presents problems due to its poor solubility and high volatility. The present study aimed to evaluate in vitro antibiofilm activity of TTO nanoparticles against many Candida species. It was performed the characterization of the oil and nanoparticles. The levels of exopolysaccharides, proteins, and the biomass of biofilms were measured. The chromatographic profile demonstrated that the TTO oil is in accordance with ISO 4730 with major constituents of 41.9% Terpinen-4-ol, 20.1% of γ-Terpinene, 9,8% of α-Terpinene, and 6,0% of 1,8-Cineole. The TTO nanoparticles showed pH of 6.3, mean diameter of 158.2 ± 2 nm, polydispersion index of 0.213 ± 0.017, and zeta potential of -8.69 ± 0.80 mV. The addition of TTO and its nanoparticles represented a significant reduction of biofilm formed by all Candida species, as well as a reduction of proteins and exopolysaccharides levels. It was possible to visualize the reduction of biofilm in presence of TTO nanoparticles by Calcofluor White method.
[Mh] Termos MeSH primário: Antifúngicos/administração & dosagem
Biofilmes/efeitos dos fármacos
Candida/efeitos dos fármacos
Candida/fisiologia
Melaleuca/química
Nanopartículas/administração & dosagem
Extratos Vegetais/administração & dosagem
[Mh] Termos MeSH secundário: Antifúngicos/química
Proteínas de Bactérias/metabolismo
Biomassa
Candida/ultraestrutura
Testes de Sensibilidade Microbiana
Nanopartículas/química
Extratos Vegetais/química
Óleos Vegetais/administração & dosagem
Óleos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Bacterial Proteins); 0 (Plant Extracts); 0 (Plant Oils)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170406
[Lr] Data última revisão:
170406
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170117
[St] Status:MEDLINE


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[PMID]:27988310
[Au] Autor:Baldissera MD; Souza CF; Júnior GB; de Vargas AC; Boligon AA; de Campos MM; Stefani LM; Baldisserotto B
[Ad] Endereço:Department of Microbiology and Parasitology, Universidade Federal de Santa Maria, Santa Maria, RS, Brazil. Electronic address: matheusd.biomed@yahoo.com.br.
[Ti] Título:Melaleuca alternifolia essential oil enhances the non-specific immune system and prevents oxidative damage in Rhamdia quelen experimentally infected by Aeromonas hydrophila: Effects on cholinergic and purinergic systems in liver tissue.
[So] Source:Fish Shellfish Immunol;61:1-8, 2017 Feb.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to evaluate the effects of M. alternifolia essential oil used to treat silver catfish (Rhamdia quelen) experimentally infected by Aeromonas hydrophila on oxidative stress variables, and for the first time, on hepatic enzymes of the cholinergic and adenosinergic systems. For that, fish were divided into six groups (A-F), each containing seven animals. Groups A, B and C were composed of uninfected animals, while animals in groups D, E and F were intramuscularly inoculated with A. hydrophila. Groups B and E received a prophylactic bath with M. alternifolia essential oil (50 µL/L, diluted in ethanol) for seven days, while groups C and F were exposed to ethanol. After the prophylactic baths, groups D, E and F were inoculated with 100 µL of A. hydrophila solution (2.1 × 10 colony-forming unit). Two days after inoculation, the animals were euthanized and liver samples were collected. Infected animals (the group D) showed increased TBARS and protein carbonylation levels, while CAT, AChE and ADA activities decreased compared to uninfected animals (the group A). The prophylactic treatment with M. alternifolia essential oil (the group E) prevented the alterations caused by A. hydrophila, but it did not change AChE activity. Thus, the prophylactic treatment prevents damage caused by lipids and proteins, as well as alterations of the adenosinergic system, demonstrating that the anti-inflammatory effect of TTO is mediated by the adenosinergic pathway. In addition, TTO prophylactic treatment might be considered an important approach to prevent the hepatic damage caused by A. hydrophila.
[Mh] Termos MeSH primário: Peixes-Gato
Doenças dos Peixes/genética
Infecções por Bactérias Gram-Negativas/veterinária
Melaleuca/química
Estresse Oxidativo/efeitos dos fármacos
Óleo de Melaleuca/farmacologia
[Mh] Termos MeSH secundário: Aeromonas hydrophila/fisiologia
Animais
Doenças dos Peixes/imunologia
Doenças dos Peixes/microbiologia
Infecções por Bactérias Gram-Negativas/genética
Infecções por Bactérias Gram-Negativas/imunologia
Infecções por Bactérias Gram-Negativas/microbiologia
Imunidade Inata/efeitos dos fármacos
Fígado/efeitos dos fármacos
Fígado/metabolismo
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
68647-73-4 (Tea Tree Oil)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161219
[St] Status:MEDLINE


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[PMID]:27762176
[Au] Autor:Monteiro DU; Azevedo MI; Weiblen C; DE Avila Botton S; Funk NL; DE Bona DA Silva C; Zanette RA; Schwanz TG; DE LA Rue ML
[Ad] Endereço:Postgraduation Program in Pharmaceutical Sciences, Universidade Federal de Santa Maria, UFSM,Santa Maria,Brazil.
[Ti] Título:In vitro and ex vivo activity of Melaleuca alternifolia against protoscoleces of Echinococcus ortleppi.
[So] Source:Parasitology;144(2):214-219, 2017 Feb.
[Is] ISSN:1469-8161
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cystic echinococcosis is a zoonotic disease of difficult diagnosis and treatment. The use of protoscolicidal agents in procedures is of utmost importance for treatment success. This study was aimed at analysing the in vitro and ex vivo activity of Melaleuca alternifolia oil (tea tree oil - TTO), its nanoemulsion formulation (NE-TTO) and its major component (terpinen-4-ol) against Echinococcus ortleppi protoscoleces obtained from cattle. Concentrations of 2·5, 5 and 10 mg mL-1 of TTO, 10 mg mL-1 of NE-TTO and 1, 1·5 and 2 mg mL-1 of terpinen-4-ol were evaluated in vitro against protoscoleces at 5, 10, 15 and 30 min. TTO was also injected directly into hydatid cysts (ex vivo analysis, n = 20) and the viability of protoscoleces was evaluated at 5, 15 and 30 min. The results indicated protoscolicidal effect at all tested formulations and concentrations. Terpinen-4-ol (2 mg mL-1) activity was superior when compared with the highest concentration of TTO. NE-TTO reached a gradual protoscolicidal effect. TTO at 20 mg mL-1 showed 90% protoscolicidal action in hydatid cysts at 5 min. The results showed that TTO affects the viability of E. ortleppi protoscoleces, suggesting a new protoscolicidal option to the treatment of cystic equinococcosis.
[Mh] Termos MeSH primário: Anti-Helmínticos/farmacologia
Echinococcus/efeitos dos fármacos
Melaleuca
Óleo de Melaleuca/farmacologia
[Mh] Termos MeSH secundário: Animais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 68647-73-4 (Tea Tree Oil)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE
[do] DOI:10.1017/S0031182016001621


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[PMID]:28076463
[Au] Autor:Graziano TS; Calil CM; Sartoratto A; Franco GC; Groppo FC; Cogo-Müller K
[Ad] Endereço:- Universidade Estadual de Campinas, Faculdade de Odontologia de Piracicaba, Área de Microbiologia e Imunologia, Departamento de Diagnóstico Oral, Piracicaba, SP, Brasil.
[Ti] Título:In vitro effects of Melaleuca alternifolia essential oil on growth and production of volatile sulphur compounds by oral bacteria.
[So] Source:J Appl Oral Sci;24(6):582-589, 2016 Nov-Dec.
[Is] ISSN:1678-7765
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Objective: Halitosis can be caused by microorganisms that produce volatile sulphur compounds (VSCs), which colonize the surface of the tongue and subgingival sites. Studies have reported that the use of natural products can reduce the bacterial load and, consequently, the development of halitosis. The aim of this study was to evaluate the antimicrobial activity of the essential oil of Melaleuca alternifolia on the growth and volatile sulphur compound (VSC) production of oral bacteria compared with chlorhexidine. Material and Methods: The effects of these substances were evaluated by the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) in planktonic cultures of Porphyromonas gingivalis and Porphyromonas endodontalis. In addition, gas chromatography analyses were performed to measure the concentration of VSCs from bacterial cultures and to characterize M. alternifolia oil components. Results: The MIC and MBC values were as follows: M. alternifolia - P. gingivalis (MIC and MBC=0.007%), P. endodontalis (MIC and MBC=0.007%=0.5%); chlorhexidine - P. gingivalis and P. endodontalis (MIC and MBC=1.5 mg/mL). M. alternifolia significantly reduced the growth and production of hydrogen sulfide (H2S) by P. gingivalis (p<0.05, ANOVA-Dunnet) and the H2S and methyl mercaptan (CH3SH) levels of P. endodontalis (p<0.05, ANOVA-Dunnet). Chlorhexidine reduced the growth of both microorganisms without altering the production of VSC in P. endodontalis. For P. gingivalis, the production of H2S and CH3SH decreased (p<0.05, ANOVA-Dunnet). Conclusion: M. alternifolia can reduce bacterial growth and VSCs production and could be used as an alternative to chlorhexidine.
[Mh] Termos MeSH primário: Antibacterianos/farmacologia
Melaleuca/química
Porphyromonas endodontalis/efeitos dos fármacos
Porphyromonas gingivalis/efeitos dos fármacos
Compostos de Enxofre/metabolismo
Óleo de Melaleuca/farmacologia
[Mh] Termos MeSH secundário: Análise de Variância
Carga Bacteriana/efeitos dos fármacos
Células Cultivadas
Cromatografia Gasosa-Espectrometria de Massas
Halitose/metabolismo
Halitose/microbiologia
Halitose/prevenção & controle
Testes de Sensibilidade Microbiana
Porphyromonas endodontalis/crescimento & desenvolvimento
Porphyromonas endodontalis/metabolismo
Porphyromonas gingivalis/crescimento & desenvolvimento
Porphyromonas gingivalis/metabolismo
Reprodutibilidade dos Testes
Compostos de Enxofre/análise
Fatores de Tempo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Sulfur Compounds); 68647-73-4 (Tea Tree Oil)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170430
[Lr] Data última revisão:
170430
[Sb] Subgrupo de revista:D; IM
[Da] Data de entrada para processamento:170112
[St] Status:MEDLINE


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[PMID]:27936192
[Au] Autor:Liao M; Xiao JJ; Zhou LJ; Liu Y; Wu XW; Hua RM; Wang GR; Cao HQ
[Ad] Endereço:School of Plant Protection, Anhui Agricultural University, Hefei, China.
[Ti] Título:Insecticidal Activity of Melaleuca alternifolia Essential Oil and RNA-Seq Analysis of Sitophilus zeamais Transcriptome in Response to Oil Fumigation.
[So] Source:PLoS One;11(12):e0167748, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The cereal weevil, Sitophilus zeamais is one of the most destructive pests of stored cereals worldwide. Frequent use of fumigants for managing stored-product insects has led to the development of resistance in insects. Essential oils from aromatic plants including the tea oil plant, Melaleuca alternifolia may provide environmentally friendly alternatives to currently used pest control agents. However, little is known about molecular events involved in stored-product insects in response to plant essential oil fumigation. RESULTS: M. alternifolia essential oil was shown to possess the fumigant toxicity against S. zeamais. The constituent, terpinen-4-ol was the most effective compound for fumigant toxicity. M. alternifolia essential oil significantly inhibited the activity of three enzymes in S. zeamais, including two detoxifying enzymes, glutathione S-transferase (GST), and carboxylesterase (CarE), as well as a nerve conduction enzyme, acetylcholinesterase (AChE). Comparative transcriptome analysis of S. zeamais through RNA-Seq identified a total of 3,562 differentially expressed genes (DEGs), of which 2,836 and 726 were up-regulated and down-regulated in response to M. alternifolia essential oil fumigation, respectively. Based on gene ontology (GO) analysis, the majority of DEGs were involved in insecticide detoxification and mitochondrial function. Furthermore, an abundance of DEGs mapped into the metabolism pathway in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database were associated with respiration and metabolism of xenobiotics, including cytochrome P450s, CarEs, GSTs, and ATP-binding cassette transporters (ABC transporters). Some DEGs mapped into the proteasome and phagosome pathway were found to be significantly enriched. These results led us to propose a model of insecticide action that M. alternifolia essential oil likely directly affects the hydrogen carrier to block the electron flow and interfere energy synthesis in mitochondrial respiratory chain. CONCLUSION: This is the first study to perform a comparative transcriptome analysis of S. zeamais in response to M. alternifolia essential oil fumigation. Our results provide new insights into the insecticidal mechanism of M. alternifolia essential oil fumigation against S. zeamais and eventually contribute to the management of this important agricultural pest.
[Mh] Termos MeSH primário: Fumigação/métodos
Melaleuca/química
Óleos Voláteis/toxicidade
Óleos Vegetais/toxicidade
Transcriptoma/efeitos dos fármacos
Gorgulhos/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Regulação da Expressão Gênica/efeitos dos fármacos
Óleos Voláteis/química
Óleos Vegetais/química
Gorgulhos/enzimologia
Gorgulhos/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Oils, Volatile); 0 (Plant Oils)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0167748


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[PMID]:27646626
[Au] Autor:Grando TH; Baldissera MD; Gressler LT; de Sá MF; Bortoluzzi BN; Schafer AS; Ebling RC; Raffin RP; Santos RCV; Stefani LM; Vaucher R; Leal MLR; Monteiro SG
[Ad] Endereço:Department of Microbiology and Parasitology, Universidade Federal Santa Maria - UFSM, Santa Maria, Rio Grande do Sul, Brazil. Electronic address: thirssa_thi@hotmail.com.
[Ti] Título:Melaleuca alternifolia anthelmintic activity in gerbils experimentally infected by Haemonchus contortus.
[So] Source:Exp Parasitol;170:177-183, 2016 Nov.
[Is] ISSN:1090-2449
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Gastrointestinal parasites are one of the biggest health problems faced in sheep, mainly due to their pathogenicity and resistance to drugs used to control these parasites. Thus, the following study aimed to assess the anthelmintic efficacy of Melaleuca alternifolia against Haemonchus contortus in gerbils (Meriones unguiculatus) experimentally infected. Three treatments were tested: M. alternifolia essential oil, popularly known as tea tree oil (TTO), a solid lipid nanocarrier made with essential oil of Melaleuca (nanoTTO), and terpinen-4-ol (terp-4-ol). In vivo studies were performed by determining the mean worm burden of H. contortus in gerbils TTO (0.75 mL/kg); nanoTTO (0.5 mL/kg) and terp-4-ol (0.5 mL l/kg) were able to reduce 46.36%; 48.64%, and 43.18% worm burden, respectively. H. contortus increased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, as demonstrated by liver injury. It was found that the TTO, nanoTTO, and terp-4-ol were not toxic to liver and kidneys since hepatic and renal functions were not affected. Moreover, terp-4-ol was able to prevent increased levels of seric AST and ALT in infected animals, indicating a hepatoprotective effect. Thus, our results indicate that TTO, nanoTTO, and terp-4-ol are safe and efficient against H. contortus infection in gerbils, and possibly the terp-4-ol may be considered the compound present in the Melaleuca alternifolia responsible for parasitic action against H. contortus.
[Mh] Termos MeSH primário: Anti-Helmínticos/farmacologia
Hemoncose/tratamento farmacológico
Haemonchus/efeitos dos fármacos
Melaleuca/química
Óleo de Melaleuca/farmacologia
Terpenos/farmacologia
[Mh] Termos MeSH secundário: Alanina Transaminase/sangue
Animais
Anti-Helmínticos/administração & dosagem
Anti-Helmínticos/química
Aspartato Aminotransferases/sangue
Análise Química do Sangue
Modelos Animais de Doenças
Feminino
Cromatografia Gasosa-Espectrometria de Massas
Gerbillinae
Lipídeos
Masculino
Nanocápsulas
Distribuição Aleatória
Óleo de Melaleuca/administração & dosagem
Óleo de Melaleuca/química
Terpenos/administração & dosagem
Terpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthelmintics); 0 (Lipids); 0 (Nanocapsules); 0 (Terpenes); 562-74-3 (terpinenol-4); 68647-73-4 (Tea Tree Oil); EC 2.6.1.1 (Aspartate Aminotransferases); EC 2.6.1.2 (Alanine Transaminase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160921
[St] Status:MEDLINE



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