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Pesquisa : B01.650.940.800.575.912.250.812.666.500 [Categoria DeCS]
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[PMID]:29257634
[Au] Autor:Arulmathi P; Elangovan G
[Ti] Título:Applications of response surface methodology and artificial neural network for decolorization of distillery spent wash by using activated Piper nigrum.
[So] Source:J Environ Biol;37(6):1255-63, 2016 Nov.
[Is] ISSN:0254-8704
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Ethanol production from sugarcane molasses yields large volume of highly colored spent wash as effluent. This color is imparted by the recalcitrant melanoidin pigment produced due to the Maillard reaction. In the present work, decolourization of melanoidin was carried out using activated carbon prepared from pepper stem (Piper nigrum). The interaction effect between parameters were studied by response surface methodology using central composite design and maximum decolourization of 75 % was obtained at pH 7.5, Melanoidin concentration of 32.5 mg l-1 with 1.63 g 100ml-1 of adsorbent for 2hr 75min. Artificial neural networks was also used to optimize the process parameters, giving 74 % decolourization for the same parameters. The Langmuir and Freundich isotherms were applied for describing the biosorption equilibrium. The process was represented by the Langmuir isotherm with a correlation coefficient of 0.94. The first-order, second-order models were implemented for demonstrating the biosorption mechanism and, as a result, Pseudo second order model kinetics fitted best to the experimental data. The estimated enthalpy change (DH) and entropy change (DS) of adsorption were 32.195 kJ mol-1 and 115.44 J mol-1 K which indicates that the adsorption of melanoidin was an endothermic process. Continuous adsorption studies were conducted under optimized condition. The breakthrough curve analysis was determined using the experimental data obtained from continuous adsorption. Continuous column studies gave a breakthrough at 182 mins and 176 ml. It was concluded that column packed with Piper nigrum based activated carbon can be used to remove color from distillery spent wash.
[Mh] Termos MeSH primário: Resíduos Industriais/análise
Redes Neurais (Computação)
Piper nigrum/química
Eliminação de Resíduos Líquidos/métodos
[Mh] Termos MeSH secundário: Adsorção
Indústria Alimentícia
Polímeros/química
Termodinâmica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Industrial Waste); 0 (Polymers); 0 (melanoidin polymers)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180219
[Lr] Data última revisão:
180219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171220
[St] Status:MEDLINE


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[PMID]:29248443
[Au] Autor:Jin Z; Kwon M; Lee AR; Ro DK; Wungsintaweekul J; Kim SU
[Ad] Endereço:Department of Agricultural Biotechnology and Research Institute for Agricultural Sciences, Seoul National University, Seoul 08826, Republic of Korea.
[Ti] Título:Molecular cloning and functional characterization of three terpene synthases from unripe fruit of black pepper (Piper nigrum).
[So] Source:Arch Biochem Biophys;638:35-40, 2018 01 15.
[Is] ISSN:1096-0384
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:To identify terpene synthases (TPS) responsible for the biosynthesis of the sesquiterpenes that contribute to the characteristic flavors of black pepper (Piper nigrum), unripe peppercorn was subjected to the Illumina transcriptome sequencing. The BLAST analysis using amorpha-4,11-diene synthase as a query identified 19 sesquiterpene synthases (sesqui-TPSs), of which three full-length cDNAs (PnTPS1 through 3) were cloned. These sesqui-TPS cDNAs were expressed in E. coli to produce recombinant enzymes for in vitro assays, and also expressed in the engineered yeast strain to assess their catalytic activities in vivo. PnTPS1 produced ß-caryophyllene as a main product and humulene as a minor compound, and thus was named caryophyllene synthase (PnCPS). Likewise, PnTPS2 and PnTPS3 were, respectively, named cadinol/cadinene synthase (PnCO/CDS) and germacrene D synthase (PnGDS). PnGDS expression in yeast yielded ß-cadinene and α-copaene, the rearrangement products of germacrene D. Their k /K values (20-37.7 s mM ) were comparable to those of other sesqui-TPSs. Among three PnTPSs, the transcript level of PnCPS was the highest, correlating with the predominant ß-caryophyllene biosynthesis in the peppercorn. The products and rearranged products of three PnTPSs could account for about a half of the sesquiterpenes in number found in unripe peppercorn.
[Mh] Termos MeSH primário: Alquil e Aril Transferases
Clonagem Molecular
Frutas
Piper nigrum
Proteínas de Plantas
[Mh] Termos MeSH secundário: Alquil e Aril Transferases/genética
Alquil e Aril Transferases/metabolismo
DNA Complementar/genética
Frutas/enzimologia
Frutas/genética
Piper nigrum/enzimologia
Piper nigrum/genética
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Sesquiterpenos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Complementary); 0 (Plant Proteins); 0 (Sesquiterpenes); 54W56MD2WD (humulene); BHW853AU9H (caryophyllene); EC 2.5.- (Alkyl and Aryl Transferases); EC 2.5.1.- (terpene synthase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180214
[Lr] Data última revisão:
180214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171218
[St] Status:MEDLINE


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[PMID]:28591150
[Au] Autor:Sousa JO; Suz LM; García MA; Alfredo DS; Conrado LM; Marinho P; Ainsworth AM; Baseia IG; Martín MP
[Ad] Endereço:Programa de Pós-Graduação em Sistemática e Evolução, Universidade Federal do Rio Grande do Norte, Natal, Rio Grande do Norte, Brazil.
[Ti] Título:More than one fungus in the pepper pot: Integrative taxonomy unmasks hidden species within Myriostoma coliforme (Geastraceae, Basidiomycota).
[So] Source:PLoS One;12(6):e0177873, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Since the nineteenth century, Myriostoma has been regarded as a monotypic genus with a widespread distribution in north temperate and subtropical regions. However, on the basis of morphological characters and phylogenetic evidence of DNA sequences of the internal transcribed spacer (ITS) regions and the large subunit nuclear ribosomal RNA gene (LSU), four species are now delimited: M. areolatum comb. & stat. nov., M. calongei sp. nov., M. capillisporum comb. & stat. nov., and M. coliforme. Myriostoma coliforme is typified by selecting a lectotype (iconotype) and a modern sequenced collection as an epitype. The four species can be discriminated by a combination of morphological characters, such as stomatal form, endoperidial surface texture, and basidiospore size and ornamentation.
[Mh] Termos MeSH primário: Basidiomycota/genética
DNA Espaçador Ribossômico/genética
Filogenia
[Mh] Termos MeSH secundário: Basidiomycota/classificação
Basidiomycota/patogenicidade
Classificação
DNA Fúngico/genética
Piper nigrum/microbiologia
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Fungal); 0 (DNA, Ribosomal Spacer)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177873


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[PMID]:28407236
[Au] Autor:Duncan SE; Moberg K; Amin KN; Wright M; Newkirk JJ; Ponder MA; Acuff GR; Dickson JS
[Ad] Endereço:Food Science and Technology Dept., Human and Agricultural Biosciences Building, 1230 Washington St. SW., Virginia Tech, Blacksburg, Va, 24061, U.S.A.
[Ti] Título:Processes to Preserve Spice and Herb Quality and Sensory Integrity During Pathogen Inactivation.
[So] Source:J Food Sci;82(5):1208-1215, 2017 May.
[Is] ISSN:1750-3841
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Selected processing methods, demonstrated to be effective at reducing Salmonella, were assessed to determine if spice and herb quality was affected. Black peppercorn, cumin seed, oregano, and onion powder were irradiated to a target dose of 8 kGy. Two additional processes were examined for whole black peppercorns and cumin seeds: ethylene oxide (EtO) fumigation and vacuum assisted-steam (82.22 °C, 7.5 psia). Treated and untreated spices/herbs were compared (visual, odor) using sensory similarity testing protocols (α = 0.20; ß = 0.05; proportion of discriminators: 20%) to determine if processing altered sensory quality. Analytical assessment of quality (color, water activity, and volatile chemistry) was completed. Irradiation did not alter visual or odor sensory quality of black peppercorn, cumin seed, or oregano but created differences in onion powder, which was lighter (higher L ) and more red (higher a ) in color, and resulted in nearly complete loss of measured volatile compounds. EtO processing did not create detectable odor or appearance differences in black peppercorn; however visual and odor sensory quality differences, supported by changes in color (higher b ; lower L ) and increased concentrations of most volatiles, were detected for cumin seeds. Steam processing of black peppercorn resulted in perceptible odor differences, supported by increased concentration of monoterpene volatiles and loss of all sesquiterpenes; only visual differences were noted for cumin seed. An important step in process validation is the verification that no effect is detectable from a sensory perspective.
[Mh] Termos MeSH primário: Cuminum/química
Óxido de Etileno/farmacologia
Piper nigrum/química
Especiarias/análise
Vapor
[Mh] Termos MeSH secundário: Monoterpenos/análise
Sementes/química
Sesquiterpenos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Monoterpenes); 0 (Sesquiterpenes); 0 (Steam); JJH7GNN18P (Ethylene Oxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE
[do] DOI:10.1111/1750-3841.13702


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[PMID]:28314475
[Au] Autor:Chen Z; Sun D; Bi X; Zeng X; Luo W; Cai D; Zeng Q; Xu A
[Ad] Endereço:Guangdong Province Engineering Technology Research Institute of T.C.M., 60 Hengfu rd., Guangzhou, 510095, China; Affiliated Guangdong second TCM hospital, Guangzhou University of Chinese Medicine, 60 Hengfu rd., Guangzhou, 510095, China; Guangdong Provincial Key Laboratory of Research and Developmen
[Ti] Título:Pharmacokinetic based study on "lagged stimulation" of Curcumae Longae Rhizoma - Piper nigrum couplet in their main active components' metabolism using UPLC-MS-MS.
[So] Source:Phytomedicine;27:15-22, 2017 Apr 15.
[Is] ISSN:1618-095X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Curcumae Longae Rhizoma is one of the commonly used traditional Chinese medicines, which has multiple biological activities such as relieving stagnation and stasis, pain alleviation, curing amenorrhea and wounds. However, its main active component-curcumin has poor absorption and very fast metabolism in body. To solve this problem, Piper nigrum was introduced for its ability to strengthen bioavailability of other compounds. PURPOSE: In most cases of TCM couplets, all ingredients were prepared and taken simultaneously, which in our opinion did not take full advantage of their interactions. Therefore, order of administration should be adjusted according to pharmacokinetic parameters of the ingredients, which the ones act as supplement can first be taken, and main therapeutic components followed when the former reached its peak. METHOD: the extract of Piper nigrum (containing at least 95% piperine) was taken by rats 6h before taking Curcumae Longae Rhizoma extract (containing at least 95% curcumin). Then, a UPLC-MS-MS method was developed to determine their content in plasma simultaneously. Determination was carried out by on a C18 column within 5min by isocratic elution using 0.2% formic acid and acetonitrile (50:50, v/v). Tandem mass detection was conducted by selective reaction monitoring (SRM) via electrospray ionization (ESI) source in positive mode. Samples were pre-treated by liquid-liquid extraction (LLE), and verapamil was used as internal standard (IS). RESULTS: For both curcumin and piperine, the proposed method had good linearity (r =0.999) within the concentration range of 1-1000ng/ml, with good recovery, precision and stability. The lower limit of quantification (LLOQ) was 1ng/ml. As pharmacokinetic data indicated, Maximum concentration (C ) of curcumin increased significantly to 394.06; the time reach maximum concentration (T ) and elimination half-life (T ) were 0.5 and 0.67h, respectively; CONCLUSION: The results provide a good strategy for the investigation of TCM formula especially the couplets, as well as a fast, selective and sensitive UPLC-MS-MS method determining active components in-vivo. Furthermore, the finding of "lagged stimulation" suggested that the use of complex formula should take pharmacokinetics into much more careful consideration.
[Mh] Termos MeSH primário: Curcuma/metabolismo
Curcumina/farmacocinética
Inibidores das Enzimas do Citocromo P-450/farmacocinética
Piper nigrum/metabolismo
Piperidinas/farmacocinética
Extratos Vegetais/farmacocinética
[Mh] Termos MeSH secundário: Animais
Cromatografia Líquida de Alta Pressão/métodos
Curcuma/química
Medicamentos de Ervas Chinesas/farmacocinética
Masculino
Piper nigrum/química
Ratos
Ratos Sprague-Dawley
Rizoma/química
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytochrome P-450 Enzyme Inhibitors); 0 (Drugs, Chinese Herbal); 0 (Piperidines); 0 (Plant Extracts); IT942ZTH98 (Curcumin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170522
[Lr] Data última revisão:
170522
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170319
[St] Status:MEDLINE


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[PMID]:28260140
[Au] Autor:Huang KS; Tai CH; Cheng YH; Lin SH; Chen TC; Jan FJ
[Ad] Endereço:Department of Plant Pathology, National Chung Hsing University, Taichung, 40227, Taiwan.
[Ti] Título:Complete nucleotide sequences of M and L RNAs from a new pepper-infecting tospovirus, Pepper chlorotic spot virus.
[So] Source:Arch Virol;162(7):2109-2113, 2017 Jul.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Pepper chlorotic spot virus (PCSV), newly found in Taiwan, was identified as a new tospovirus based on the molecular characterization of its S RNA. In this study, the complete M and L RNA sequences of PCSV were determined. The M RNA has 4795 nucleotides (nts), encoding the NSm protein of 311 aa (34.5 kDa) in the viral (v) strand and the glycoprotein precursor (Gn/Gc) of 1122 aa (127.6 kDa) in the viral complementary (vc) strand. The L RNA has 8859 nts, encoding the RNA-dependent RNA polymerase (RdRp) of 2873 aa (330.8 kDa) in the vc strand. Analyses of the NSm, Gn/Gc and RdRp of PCSV revealed that PCSV is phylogenetically clustered within the watermelon silver mottle virus-related clade. Based on the whole genome sequence, PCSV is closely related to Tomato necrotic ringspot virus and should be classified as a new tospovirus species.
[Mh] Termos MeSH primário: Piper nigrum/virologia
Doenças das Plantas/virologia
RNA Replicase/genética
RNA Viral/genética
Tospovirus/classificação
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Lycopersicon esculentum/virologia
Filogenia
Taiwan
Tospovirus/genética
Tospovirus/isolamento & purificação
Proteínas Virais/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Viral); 0 (Viral Proteins); EC 2.7.7.48 (RNA Replicase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170306
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3260-1


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[PMID]:28254575
[Au] Autor:Khawas S; Nosálová G; Majee SK; Ghosh K; Raja W; Sivová V; Ray B
[Ad] Endereço:Natural Products Laboratory, Department of Chemistry, The University in Burdwan, West Bengal, India.
[Ti] Título:In vivo cough suppressive activity of pectic polysaccharide with arabinogalactan type II side chains of Piper nigrum fruits and its synergistic effect with piperine.
[So] Source:Int J Biol Macromol;99:335-342, 2017 Jun.
[Is] ISSN:1879-0003
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Piper nigrum L. fruits are not only a prized spice, but also highly valued therapeutic agent that heals many ailments including asthma, cold and respiratory problems. Herein, we have investigated structural features and in vivo antitussive activity of three fractions isolated from Piper nigrum fruits. The water extract (PN-WE) upon fractionation with EtOH yielded two fractions: a soluble fraction (PN-eSf) and a precipitated (PN-ePf) one. The existence of a pectic polysaccharide with arabinogalactan type II side chains (147kDa) in PN-ePf and piperine in PN-eSf were revealed. Moreover, oligosaccharides providing fine structural details of side chains were generated from PN-ePf and then characterized. The parental water extract (PN-WE) that contained both pectic polysaccharide and piperine, after oral administration (50mgkg body weight) to guinea pigs, showed antitussive activity comparable to codeine phosphate (10mgkg body weight). The EtOH precipitated fraction (PN-ePf) containing pectic polysaccharide showed comparatively higher antitussive activity than EtOH soluble fraction (PN-eSf) that contained piperine, but their potencies are lower than the parental water extract. Significantly, the specific airway smooth muscle reactivity of all three fractions remained unchanged. Finally, pectic polysaccharide-piperine combination in parental extract synergistically enhances antitussive effect in guinea pigs.
[Mh] Termos MeSH primário: Tosse/tratamento farmacológico
Frutas/química
Galactanos/química
Pectinas/química
Pectinas/farmacologia
Piper nigrum/química
Piperidinas/farmacologia
[Mh] Termos MeSH secundário: Animais
Antitussígenos/química
Antitussígenos/isolamento & purificação
Antitussígenos/farmacologia
Antitussígenos/uso terapêutico
Sinergismo Farmacológico
Etanol/química
Cobaias
Masculino
Monossacarídeos/análise
Pectinas/isolamento & purificação
Pectinas/uso terapêutico
Reflexo/efeitos dos fármacos
Sistema Respiratório/efeitos dos fármacos
Sistema Respiratório/fisiopatologia
Solubilidade
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antitussive Agents); 0 (Galactans); 0 (Monosaccharides); 0 (Pectins); 0 (Piperidines); 059QF0KO0R (Water); 3K9958V90M (Ethanol); SL4SX1O487 (arabinogalactan)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE


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[PMID]:28221981
[Au] Autor:Hildebrandt IM; Hu C; Grasso-Kelley EM; Ye P; Anderson NM; Keller SE
[Ad] Endereço:U.S. Food and Drug Administration, 6502 South Archer Road, Bedford Park, Illinois 60501.
[Ti] Título:Dry Transfer Inoculation of Low-Moisture Spices Containing Antimicrobial Compounds.
[So] Source:J Food Prot;80(2):338-344, 2017 Feb.
[Is] ISSN:1944-9097
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Inoculation of a food product for use in subsequent validation studies typically makes use of a high concentration cocktail of microorganisms suspended in aqueous media. However, this inoculation method may prove difficult particularly when the food product is a low-moisture food containing antimicrobial compounds, such as some dried spices. In this study, a dry transfer method for inoculation of clove powder, oregano leaves, ginger powder, and ground black pepper with a five-serovar cocktail of Salmonella was developed and compared with a traditional aqueous inoculation procedure. Spices were inoculated at three levels, 10, 8, and 6 log CFU/g, by using both an aqueous suspension of Salmonella and a dry transfer of Salmonella from previously inoculated silica beads. At the highest inoculation level, the dry transfer method resulted in a significantly higher microbial load (P < 0.05) for ground cloves and oregano, but not for ginger and ground black pepper. At the intermediate inoculation level, differences were apparent only for ginger and black pepper. Inoculation levels of 6 log CFU/g resulted in recoveries below detection limits for both methods of inoculation. Additional examination on the survival of Salmonella on silica beads after inoculation and in clove powder after dry transfer from silica beads showed linear rates of decline, with a rate of -0.011 log CFU/g/day for beads and -0.015 log CFU/g/day for clove powder. The results suggest that dry transfer of Salmonella via inoculated silica beads is a viable alternative when traditional aqueous inoculation is not feasible.
[Mh] Termos MeSH primário: Contagem de Colônia Microbiana
Especiarias
[Mh] Termos MeSH secundário: Anti-Infecciosos
Microbiologia de Alimentos
Piper nigrum
Salmonella
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170729
[Lr] Data última revisão:
170729
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE
[do] DOI:10.4315/0362-028X.JFP-16-279


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[PMID]:28185326
[Au] Autor:Ngo QM; Tran PT; Tran MH; Kim JA; Rho SS; Lim CH; Kim JC; Woo MH; Choi JS; Lee JH; Min BS
[Ad] Endereço:College of Pharmacy, Drug Research, and Development Center, Catholic University of Daegu, Gyeongbuk, 38430, Korea.
[Ti] Título:Alkaloids from Piper nigrum Exhibit Antiinflammatory Activity via Activating the Nrf2/HO-1 Pathway.
[So] Source:Phytother Res;31(4):663-670, 2017 Apr.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the present study, ten alkaloids, namely chabamide (1), pellitorine (2), retrofractamide A (3), pyrroperine (4), isopiperolein B (5), piperamide C9:1 (8E) (6), 6,7-dehydrobrachyamide B (7), 4,5-dihydropiperine (8), dehydropipernonaline (9), and piperine (10), were isolated from the fruits of Piper nigrum. Among these, chabamide (1), pellitorine (2), retrofractamide A (3), isopiperolein B (5), and 6,7-dehydrobrachyamide B (7) exhibited significant inhibitory activity on lipopolysaccharide-induced nitric oxide (NO) production in RAW264.7 cells, with IC values of 6.8, 14.5, 30.2, 23.7, and 38.5 µM, respectively. Furthermore, compound 1 inhibited lipopolysaccharide-induced NO production in bone marrow-derived macrophages with IC value of 9.5 µM. Consistent with NO inhibition, treatment of RAW264.7 cells with chabamide (1), pellitorine (2), and 6,7-dehydrobrachyamide B (7) suppressed expression of inducible NO synthase and cyclooxygenase-2. Chabamide (1), pellitorine (2), and 6,7-dehydrobrachyamide B (7) induced heme-oxygenase-1 expression at the transcriptional level. In addition, compound 1 induced the nuclear translocation of nuclear factor-E2-related factor 2 (Nrf2) and upregulated the expression of Nrf2 target genes, NAD(P)H:quinone oxidoreductase 1 and γ-glutamyl cysteine synthetase catalytic subunit, in a concentration-dependent manner in RAW264.7 cells. These findings suggest that chabamide (1) from P. nigrum exert antiinflammatory effects via the activation of the Nrf2/heme-oxygenase-1 pathway; hence, it might be a promising candidate for the treatment of inflammatory diseases. Copyright © 2017 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Alcaloides/química
Anti-Inflamatórios/química
Fator 2 Relacionado a NF-E2/metabolismo
Piper nigrum/química
[Mh] Termos MeSH secundário: Alcaloides/farmacologia
Animais
Anti-Inflamatórios/farmacologia
Camundongos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Anti-Inflammatory Agents); 0 (NF-E2-Related Factor 2)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170503
[Lr] Data última revisão:
170503
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5780


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[PMID]:28102026
[Au] Autor:Soutar DA; Doucette CD; Liwski RS; Hoskin DW
[Ad] Endereço:Department of Pathology, Dalhousie University, Halifax, Nova Scotia, B3H 4R2, Canada.
[Ti] Título:Piperine, a Pungent Alkaloid from Black Pepper, Inhibits B Lymphocyte Activation and Effector Functions.
[So] Source:Phytother Res;31(3):466-474, 2017 Mar.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Piperine has several well-documented anti-inflammatory properties; however, little is known regarding its effect on humoral immunity. In this study, we describe the immunosuppressive effect of piperine on B lymphocytes, which are integral to the humoral immune response. Mouse B cells were cultured in the absence or presence of non-cytotoxic concentrations (25, 50, and 100 µM) of piperine during T-dependent or T-independent stimulation. Piperine inhibited B cell proliferation by causing G0/G1 phase cell cycle arrest in association with reduced expression of cyclin D2 and D3. The inhibitory effect of piperine was not mediated through transient receptor potential vanilloid-1 ion channel (TRPV1) because piperine also inhibited the proliferation of B cells from TRPV1-deficient mice. Expression of class II major histocompatibility complex molecules and costimulatory CD40 and CD86 on B lymphocytes was reduced in the presence of piperine, as was B cell-mediated antigen presentation to syngeneic T cells. In addition, piperine inhibited B cell synthesis of interleukin (IL)-6 and IL-10 cytokines, as well as IgM, IgG2b, and IgG3 immunoglobulins. The inhibitory effect of piperine on B lymphocyte activation and effector function warrants further investigation for possible application in the treatment of pathologies related to inappropriate humoral immune responses. Copyright © 2017 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Alcaloides/farmacologia
Subpopulações de Linfócitos B/efeitos dos fármacos
Benzodioxóis/farmacologia
Ativação Linfocitária/efeitos dos fármacos
Piperidinas/farmacologia
Alcamidas Poli-Insaturadas/farmacologia
[Mh] Termos MeSH secundário: Alcaloides/isolamento & purificação
Animais
Subpopulações de Linfócitos B/fisiologia
Linfócitos B/efeitos dos fármacos
Linfócitos B/fisiologia
Benzodioxóis/isolamento & purificação
Proliferação Celular/efeitos dos fármacos
Células Cultivadas
Pontos de Checagem da Fase G1 do Ciclo Celular/efeitos dos fármacos
Interleucina-10/metabolismo
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Transgênicos
Piper nigrum/química
Piperidinas/isolamento & purificação
Alcamidas Poli-Insaturadas/isolamento & purificação
Linfócitos T/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Benzodioxoles); 0 (Piperidines); 0 (Polyunsaturated Alkamides); 130068-27-8 (Interleukin-10); U71XL721QK (piperine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170120
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5772



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