Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.822.044 [Categoria DeCS]
Referências encontradas : 162 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 17 ir para página                         

  1 / 162 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28445484
[Au] Autor:Ma Y; Shukla V; Merewitz EB
[Ad] Endereço:Department of Plant, Soil and Microbial Sciences, Michigan State University, East Lansing, Michigan, United States of America.
[Ti] Título:Transcriptome analysis of creeping bentgrass exposed to drought stress and polyamine treatment.
[So] Source:PLoS One;12(4):e0175848, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Creeping bentgrass is an important cool-season turfgrass species sensitive to drought. Treatment with polyamines (PAs) has been shown to improve drought tolerance; however, the mechanism is not yet fully understood. Therefore, this study aimed to evaluate transcriptome changes of creeping bentgrass in response to drought and exogenous spermidine (Spd) application using RNA sequencing (RNA-Seq). The high-quality sequences were assembled and 18,682 out of 49,190 (38%) were detected as coding sequences. A total of 22% and 19% of genes were found to be either up- or down-regulated due to drought while 20% and 34% genes were either up- or down- regulated in response to Spd application under drought conditions, respectively. Gene ontology (GO) and enrichment analysis were used to interpret the biological processes of transcripts and relative transcript abundance. Enriched or differentially expressed transcripts due to drought stress and/or Spd application were primarily associated with energy metabolism, transport, antioxidants, photosynthesis, signaling, stress defense, and cellular response to water deprivation. This research is the first to provide transcriptome data for creeping bentgrass under an abiotic stress using RNA-Seq analysis. Differentially expressed transcripts identified here could be further investigated for use as molecular markers or for functional analysis in responses to drought and Spd.
[Mh] Termos MeSH primário: Agrostis/genética
Secas
Poliaminas/farmacologia
Transcriptoma/efeitos dos fármacos
[Mh] Termos MeSH secundário: Agrostis/efeitos dos fármacos
Agrostis/metabolismo
Antioxidantes/metabolismo
DNA Complementar/química
DNA Complementar/metabolismo
Metabolismo Energético/efeitos dos fármacos
Perfilação da Expressão Gênica
Fotossíntese/efeitos dos fármacos
RNA/química
RNA/isolamento & purificação
RNA/metabolismo
Análise de Sequência de DNA
Estresse Fisiológico/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (DNA, Complementary); 0 (Polyamines); 63231-63-0 (RNA)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170427
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175848


  2 / 162 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28257488
[Au] Autor:Zapiola ML; Mallory-Smith CA
[Ad] Endereço:Department of Crop and Soil Science, Oregon State University, Corvallis, Oregon, United States of America.
[Ti] Título:Pollen-mediated gene flow from transgenic perennial creeping bentgrass and hybridization at the landscape level.
[So] Source:PLoS One;12(3):e0173308, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The planting of 162 ha of transgenic glyphosate-resistant creeping bentgrass (Agrostis stolonifera) near Madras, OR, USA, allowed a unique opportunity to study gene flow over time from a perennial outcrossing species at the landscape level. While conducting a four year in situ survey, we collected panicles and leaf tissue samples from creeping bentgrass and its sexually compatible species. Seeds from the panicles were planted, and seedlings were tested in the greenhouse for expression of the transgene. Gene flow via pollen was found in all four years, at frequencies of 0.004 to 2.805%. Chloroplast markers, in combination with internal transcribed spacer nuclear sequence analysis, were used to aid in identification of transgenic interspecific and intergeneric hybrid seedlings found during the testing and of established plants that could not be positively identified in the field. Interspecific transgenic hybrids produced on redtop (Agrostis gigantea) plants in situ were identified three of the four years and one intergeneric transgenic creeping bentgrass x rabbitfoot grass (Polypogon monspeliensis) hybrid was identified in 2005. In addition, we confirmed a non-transgenic creeping bentgrass x redtop hybrid in situ, demonstrating that interspecific hybrids have established in the environment outside production fields. Results of this study should be considered for deregulation of transgenic events, studies of population dynamics, and prediction of gene flow in the environment.
[Mh] Termos MeSH primário: Fluxo Gênico
Plantas Daninhas/genética
Plantas Geneticamente Modificadas/genética
Pólen/genética
[Mh] Termos MeSH secundário: Agrostis/genética
Agrostis/crescimento & desenvolvimento
Hibridização Genética
Índia
Folhas de Planta/genética
Folhas de Planta/crescimento & desenvolvimento
Plantas Daninhas/crescimento & desenvolvimento
Plantas Geneticamente Modificadas/crescimento & desenvolvimento
Pólen/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170304
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173308


  3 / 162 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28187136
[Au] Autor:Jespersen D; Belanger FC; Huang B
[Ad] Endereço:Department of Plant Biology and Pathology, Rutgers University. New Brunswick, New Jersey, United States of America.
[Ti] Título:Candidate genes and molecular markers associated with heat tolerance in colonial Bentgrass.
[So] Source:PLoS One;12(2):e0171183, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Elevated temperature is a major abiotic stress limiting the growth of cool-season grasses during the summer months. The objectives of this study were to determine the genetic variation in the expression patterns of selected genes involved in several major metabolic pathways regulating heat tolerance for two genotypes contrasting in heat tolerance to confirm their status as potential candidate genes, and to identify PCR-based markers associated with candidate genes related to heat tolerance in a colonial (Agrostis capillaris L.) x creeping bentgrass (Agrostis stolonifera L.) hybrid backcross population. Plants were subjected to heat stress in controlled-environmental growth chambers for phenotypic evaluation and determination of genetic variation in candidate gene expression. Molecular markers were developed for genes involved in protein degradation (cysteine protease), antioxidant defense (catalase and glutathione-S-transferase), energy metabolism (glyceraldehyde-3-phosphate dehydrogenase), cell expansion (expansin), and stress protection (heat shock proteins HSP26, HSP70, and HSP101). Kruskal-Wallis analysis, a commonly used non-parametric test used to compare population individuals with or without the gene marker, found the physiological traits of chlorophyll content, electrolyte leakage, normalized difference vegetative index, and turf quality were associated with all candidate gene markers with the exception of HSP101. Differential gene expression was frequently found for the tested candidate genes. The development of candidate gene markers for important heat tolerance genes may allow for the development of new cultivars with increased abiotic stress tolerance using marker-assisted selection.
[Mh] Termos MeSH primário: Agrostis/genética
Proteínas de Choque Térmico/genética
Resposta ao Choque Térmico
Proteínas de Plantas/genética
[Mh] Termos MeSH secundário: Agrostis/metabolismo
Genes de Plantas
Marcadores Genéticos
Variação Genética
Proteínas de Choque Térmico/metabolismo
Fenótipo
Proteínas de Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers); 0 (Heat-Shock Proteins); 0 (Plant Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170211
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0171183


  4 / 162 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28134592
[Au] Autor:Rioux RA; Van Ryzin BJ; Kerns JP
[Ad] Endereço:First author: Department of Plant Pathology, University of Wisconsin-Madison, Madison 53706; and second and third authors: Department of Entomology and Plant Pathology, North Carolina State University, Raleigh 27695.
[Ti] Título:Brachypodium: A Potential Model Host for Fungal Pathogens of Turfgrasses.
[So] Source:Phytopathology;107(6):749-757, 2017 06.
[Is] ISSN:0031-949X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Brachypodium distachyon is a C3 grass that is an attractive model host system for studying pathogenicity of major turfgrass pathogens due to its genetic similarity to many cool-season turfgrasses. Infection assays with two or more isolates of the casual agents of dollar spot, brown patch, and Microdochium patch resulted in compatible interactions with B. distachyon inbred line Bd21-3. The symptoms produced by these pathogens on Bd21-3 closely resembled those observed on the natural turfgrass host (creeping bentgrass), demonstrating that B. distachyon is susceptible to the fungal pathogens that cause dollar spot, brown patch, and Microdochium patch on turfgrasses. The interaction between Sclerotinia homoeocarpa isolates and Brachypodium ecotypes was also investigated. Interestingly, differential responses of these ecotypes to S. homoeocarpa isolates was found, particularly when comparing B. distachyon to B. hybridum ecotypes. Taken together, these findings demonstrate that B. distachyon can be used as a model host system for these turfgrass diseases and leveraged for studies of molecular mechanisms contributing to host resistance.
[Mh] Termos MeSH primário: Agrostis/microbiologia
Ascomicetos/fisiologia
Brachypodium/microbiologia
Modelos Biológicos
Doenças das Plantas/microbiologia
Rhizoctonia/fisiologia
[Mh] Termos MeSH secundário: Ascomicetos/patogenicidade
Ecótipo
Interações Hospedeiro-Patógeno
Rhizoctonia/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170720
[Lr] Data última revisão:
170720
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170131
[St] Status:MEDLINE
[do] DOI:10.1094/PHYTO-08-16-0318-R


  5 / 162 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28117710
[Au] Autor:Ferreira MC; Cantrell CL; Duke SO; Ali A; Rosa LH
[Ad] Endereço:Department of Microbiology, Federal University of Minas Gerais, Belo Horizonte, MG 31270-901, Brazil. marianacostaferreira@yahoo.com.br.
[Ti] Título:New Pesticidal Diterpenoids from Vellozia gigantea (Velloziaceae), an Endemic Neotropical Plant Living in the Endangered Brazilian Biome Rupestrian Grasslands.
[So] Source:Molecules;22(1), 2017 Jan 21.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:is a rare, ancient, and endemic neotropical plant present in the Brazilian Rupestrian grasslands. The dichloromethane extract of adventitious roots was phytotoxic against , , and , and showed larvicidal activity against . Phytotoxicity bioassay-directed fractionation of the extract revealed one new isopimaradiene, 8(9),15-isopimaradien-1,3,7,11-tetraone, and three new cleistanthane diterpenoids, 7-oxo-8,11,13-cleistanthatrien-3-ol, 3,20-epoxy-7-oxo-8,11,13-cleistanthatrien-3-ol, and 20-nor-3,7-dioxo-1,8,11,13-cleistanthatetraen-10-ol. These new structures are proposed based on interpretation of ¹H, C, COSY, NOESY, HSQC, and HMBC NMR data. 8(9),15-isopimaradien-1,3,7,11-tetraone was especially phytotoxic with an IC value (30 µM) comparable to those of commercial herbicides clomazone, EPTC, and naptalam. In addition, 7-oxo-8,11,13-cleistanthatrien-3-ol provided 100% mortality at a concentration of 125 ppm against one-day-old larvae. Our results show that ancient and unique plants, like the endangered narrowly endemic neotropical species present in the Rupestrian grasslands, should also be protected because they can be sources of new bioactive compounds.
[Mh] Termos MeSH primário: Diterpenos/farmacologia
Herbicidas/farmacologia
Inseticidas/farmacologia
Magnoliopsida/química
[Mh] Termos MeSH secundário: Aedes/efeitos dos fármacos
Agrostis/efeitos dos fármacos
Animais
Araceae/efeitos dos fármacos
Brasil
Diterpenos/química
Espécies em Perigo de Extinção
Herbicidas/química
Inseticidas/química
Alface/efeitos dos fármacos
Estrutura Molecular
Extratos Vegetais/química
Extratos Vegetais/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Diterpenes); 0 (Herbicides); 0 (Insecticides); 0 (Plant Extracts)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170125
[St] Status:MEDLINE


  6 / 162 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27862819
[Au] Autor:Zhang L; Routsong R; Nguyen Q; Rylott EL; Bruce NC; Strand SE
[Ad] Endereço:Department of Civil and Environmental Engineering, University of Washington, Seattle, WA, USA.
[Ti] Título:Expression in grasses of multiple transgenes for degradation of munitions compounds on live-fire training ranges.
[So] Source:Plant Biotechnol J;15(5):624-633, 2017 May.
[Is] ISSN:1467-7652
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The deposition of toxic munitions compounds, such as hexahydro-1, 3, 5-trinitro-1, 3, 5-triazine (RDX), on soils around targets in live-fire training ranges is an important source of groundwater contamination. Plants take up RDX but do not significantly degrade it. Reported here is the transformation of two perennial grass species, switchgrass (Panicum virgatum) and creeping bentgrass (Agrostis stolonifera), with the genes for degradation of RDX. These species possess a number of agronomic traits making them well equipped for the uptake and removal of RDX from root zone leachates. Transformation vectors were constructed with xplA and xplB, which confer the ability to degrade RDX, and nfsI, which encodes a nitroreductase for the detoxification of the co-contaminating explosive 2, 4, 6-trinitrotoluene (TNT). The vectors were transformed into the grass species using Agrobacterium tumefaciens infection. All transformed grass lines showing high transgene expression levels removed significantly more RDX from hydroponic solutions and retained significantly less RDX in their leaf tissues than wild-type plants. Soil columns planted with the best-performing switchgrass line were able to prevent leaching of RDX through a 0.5-m root zone. These plants represent a promising plant biotechnology to sustainably remove RDX from training range soil, thus preventing contamination of groundwater.
[Mh] Termos MeSH primário: Agrostis/genética
Biodegradação Ambiental
Panicum/genética
Plantas Geneticamente Modificadas
Triazinas/metabolismo
[Mh] Termos MeSH secundário: Agrostis/efeitos dos fármacos
Agrostis/metabolismo
Vetores Genéticos
Instalações Militares
NADH NADPH Oxirredutases/genética
Nitrorredutases/genética
Panicum/efeitos dos fármacos
Panicum/metabolismo
Fosfotransferases (Aceptor do Grupo Álcool)/genética
Poluentes do Solo/metabolismo
Poluentes do Solo/farmacocinética
Triazinas/farmacocinética
Trinitrotolueno/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil Pollutants); 0 (Triazines); 118-96-7 (Trinitrotoluene); EC 1.6.- (NADH, NADPH Oxidoreductases); EC 1.6.- (flavodoxin NADPH oxidoreductase); EC 1.7.- (Nitroreductases); EC 2.7.1.- (Phosphotransferases (Alcohol Group Acceptor)); EC 2.7.1.119 (hygromycin-B kinase); W91SSV5831 (cyclonite)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171005
[Lr] Data última revisão:
171005
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE
[do] DOI:10.1111/pbi.12661


  7 / 162 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27817970
[Au] Autor:Touceda-González M; Álvarez-López V; Prieto-Fernández Á; Rodríguez-Garrido B; Trasar-Cepeda C; Mench M; Puschenreiter M; Quintela-Sabarís C; Macías-García F; Kidd PS
[Ad] Endereço:Instituto de Investigaciones Agrobiológicas de Galicia (IIAG), Consejo Superior de Investigaciones Científicas (CSIC), Avda. de Vigo s/n, Santiago de Compostela 15705, Spain.
[Ti] Título:Aided phytostabilisation reduces metal toxicity, improves soil fertility and enhances microbial activity in Cu-rich mine tailings.
[So] Source:J Environ Manage;186(Pt 2):301-313, 2017 Jan 15.
[Is] ISSN:1095-8630
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:(Aided) phytostabilisation has been proposed as a suitable technique to decrease the environmental risks associated with metal(loid)-enriched mine tailings. Field scale evaluations are needed for demonstrating their effectiveness in the medium- to long-term. A field trial was implemented in spring 2011 in Cu-rich mine tailings in the NW of Spain. The tailings were amended with composted municipal solid wastes and planted with Salix spp., Populus nigra L. or Agrostis capillaris L. cv. Highland. Plant growth, nutritive status and metal accumulation, and soil physico- and bio-chemical properties, were monitored over three years (four years for plant growth). The total bacterial community, α- and ß-Proteobacteria, Actinobacteria and Streptomycetaceae were studied by DGGE of 16s rDNA fragments. Compost amendment improved soil properties such as pH, CEC and fertility, and decreased soil Cu availability, leading to the establishment of a healthy vegetation cover. Both compost-amendment and plant root activity stimulated soil enzyme activities and induced important shifts in the bacterial community structure over time. The woody plant, S. viminalis, and the grassy species, A. capillaris, showed the best results in terms of plant growth and biomass production. The beneficial effects of the phytostabilisation process were maintained at least three years after treatment.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Cobre/toxicidade
Microbiologia do Solo
Poluentes do Solo/toxicidade
Solo/química
[Mh] Termos MeSH secundário: Agrostis/crescimento & desenvolvimento
Bactérias/genética
Biomassa
Cobre/farmacocinética
Consórcios Microbianos/genética
Mineração
Raízes de Plantas/química
Raízes de Plantas/microbiologia
Populus/crescimento & desenvolvimento
Salix/crescimento & desenvolvimento
Poluentes do Solo/análise
Poluentes do Solo/farmacocinética
Resíduos Sólidos
Espanha
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); 0 (Soil Pollutants); 0 (Solid Waste); 789U1901C5 (Copper)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161108
[St] Status:MEDLINE


  8 / 162 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27638479
[Au] Autor:Li Z; Yuan S; Jia H; Gao F; Zhou M; Yuan N; Wu P; Hu Q; Sun D; Luo H
[Ad] Endereço:College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, China.
[Ti] Título:Ectopic expression of a cyanobacterial flavodoxin in creeping bentgrass impacts plant development and confers broad abiotic stress tolerance.
[So] Source:Plant Biotechnol J;15(4):433-446, 2017 Apr.
[Is] ISSN:1467-7652
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Flavodoxin (Fld) plays a pivotal role in photosynthetic microorganisms as an alternative electron carrier flavoprotein under adverse environmental conditions. Cyanobacterial Fld has been demonstrated to be able to substitute ferredoxin of higher plants in most electron transfer processes under stressful conditions. We have explored the potential of Fld for use in improving plant stress response in creeping bentgrass (Agrostis stolonifera L.). Overexpression of Fld altered plant growth and development. Most significantly, transgenic plants exhibited drastically enhanced performance under oxidative, drought and heat stress as well as nitrogen (N) starvation, which was associated with higher water retention and cell membrane integrity than wild-type controls, modified expression of heat-shock protein genes, production of more reduced thioredoxin, elevated N accumulation and total chlorophyll content as well as up-regulated expression of nitrite reductase and N transporter genes. Further analysis revealed that the expression of other stress-related genes was also impacted in Fld-expressing transgenics. Our data establish a key role of Fld in modulating plant growth and development and plant response to multiple sources of adverse environmental conditions in crop species. This demonstrates the feasibility of manipulating Fld in crop species for genetic engineering of plant stress tolerance.
[Mh] Termos MeSH primário: Agrostis/metabolismo
Flavodoxina/metabolismo
Plantas Geneticamente Modificadas/metabolismo
[Mh] Termos MeSH secundário: Agrostis/efeitos dos fármacos
Agrostis/genética
Secas
Flavodoxina/genética
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/genética
Temperatura Alta
Paraquat/toxicidade
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Plantas Geneticamente Modificadas/efeitos dos fármacos
Plantas Geneticamente Modificadas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavodoxin); 0 (Plant Proteins); PLG39H7695 (Paraquat)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160918
[St] Status:MEDLINE
[do] DOI:10.1111/pbi.12638


  9 / 162 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27507681
[Au] Autor:Li Z; Yu J; Peng Y; Huang B
[Ad] Endereço:Department of Grassland Science, College of Animal Science and Technology, Sichuan Agricultural University, Chengdu, 611130, China.
[Ti] Título:Metabolic pathways regulated by abscisic acid, salicylic acid and γ-aminobutyric acid in association with improved drought tolerance in creeping bentgrass (Agrostis stolonifera).
[So] Source:Physiol Plant;159(1):42-58, 2017 Jan.
[Is] ISSN:1399-3054
[Cp] País de publicação:Denmark
[La] Idioma:eng
[Ab] Resumo:Abscisic acid (ABA), salicylic acid (SA) and γ-aminobutyric acid (GABA) are known to play roles in regulating plant stress responses. This study was conducted to determine metabolites and associated pathways regulated by ABA, SA and GABA that could contribute to drought tolerance in creeping bentgrass (Agrostis stolonifera). Plants were foliar sprayed with ABA (5 µM), GABA (0.5 mM) and SA (10 µM) or water (untreated control) prior to 25 days drought stress in controlled growth chambers. Application of ABA, GABA or SA had similar positive effects on alleviating drought damages, as manifested by the maintenance of lower electrolyte leakage and greater relative water content in leaves of treated plants relative to the untreated control. Metabolic profiling showed that ABA, GABA and SA induced differential metabolic changes under drought stress. ABA mainly promoted the accumulation of organic acids associated with tricarboxylic acid cycle (aconitic acid, succinic acid, lactic acid and malic acid). SA strongly stimulated the accumulation of amino acids (proline, serine, threonine and alanine) and carbohydrates (glucose, mannose, fructose and cellobiose). GABA enhanced the accumulation of amino acids (GABA, glycine, valine, proline, 5-oxoproline, serine, threonine, aspartic acid and glutamic acid) and organic acids (malic acid, lactic acid, gluconic acid, malonic acid and ribonic acid). The enhanced drought tolerance could be mainly due to the enhanced respiration metabolism by ABA, amino acids and carbohydrates involved in osmotic adjustment (OA) and energy metabolism by SA, and amino acid metabolism related to OA and stress-defense secondary metabolism by GABA.
[Mh] Termos MeSH primário: Ácido Abscísico/farmacologia
Agrostis/fisiologia
Redes e Vias Metabólicas/efeitos dos fármacos
Reguladores de Crescimento de Planta/farmacologia
Ácido Salicílico/farmacologia
Ácido gama-Aminobutírico/farmacologia
[Mh] Termos MeSH secundário: Agrostis/efeitos dos fármacos
Secas
Regulação da Expressão Gênica de Plantas
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Growth Regulators); 56-12-2 (gamma-Aminobutyric Acid); 72S9A8J5GW (Abscisic Acid); O414PZ4LPZ (Salicylic Acid)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160811
[St] Status:MEDLINE
[do] DOI:10.1111/ppl.12483


  10 / 162 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27855226
[Au] Autor:Merewitz E; Xu Y; Huang B
[Ad] Endereço:Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, 48824, United States of America.
[Ti] Título:Differentially Expressed Genes Associated with Improved Drought Tolerance in Creeping Bentgrass Overexpressing a Gene for Cytokinin Biosynthesis.
[So] Source:PLoS One;11(11):e0166676, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Transformation with an isopentenyl transferase (ipt) gene controlling cytokinin (CK) synthesis has been shown to enhance plant drought tolerance. The objective of this study was to identify differentially-expressed genes (DEGs) in creeping bentgrass (Agrostis stolonifera) overexpressing ipt compared to non-transgenic plants. The ipt transgene was controlled by a senescence-activated promoter (SAG12). Both a null transformed line (NT) and SAG12-ipt plants were exposed to drought stress in an environmentally-controlled growth chamber until the soil water content declined to approximately 5% and leaf relative water content declined to 47%, which were both significantly below the well-watered controls. RNA was extracted from leaf samples of both well-watered and drought-stressed plants. Eight sets of subtractive hybridizations were performed for detection of up-regulated and down-regulated genes due to the presence of the transgene and due to drought stress in both NT and transgenic plants. Sequencing analysis revealed the identity of 252 DEGs due to either the transgene and drought stress. Sequencing analysis of 170 DEGs identified genes encoding for proteins that were related to energy production, metabolism, stress defense, signaling, protein synthesis and transport, and membrane transport could play major roles in the improved drought tolerance by overexpressing ipt in creeping bentgrass.
[Mh] Termos MeSH primário: Adaptação Fisiológica/genética
Agrostis/genética
Agrostis/fisiologia
Citocininas/biossíntese
Secas
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Genes de Plantas
[Mh] Termos MeSH secundário: Biblioteca Gênica
Folhas de Planta/genética
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Plantas Geneticamente Modificadas
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytokinins); 0 (Plant Proteins); 0 (RNA, Messenger)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170626
[Lr] Data última revisão:
170626
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161118
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0166676



página 1 de 17 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde