Base de dados : MEDLINE Pesquisa : B01.650.940.800.575.912.250.822.227 [Categoria DeCS] Referências encontradas : 124 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 28638886 [Au] Autor: Han X; Ji X; Zhao H; Zhang Y; Liu G; Wang Y; Zhao W; Wang S [Ad] Endereço: College of Traditional Chinese Medicine, Shandong University of Traditional Chinese Medicine, Jinan, China. [Ti] Título: MECHANISMS OF COIX SEED COMPOSITIONS IN THE TREATMENT OF SPLEEN DEFICIENCY AND WET DAMPNESS ZHENG. [So] Source: Afr J Tradit Complement Altern Med;14(4):239-246, 2017. [Is] ISSN: 2505-0044 [Cp] País de publicação: Nigeria [La] Idioma: eng [Ab] Resumo: BACKGROUND: Coix seed has the functions of fortifying the spleen and inhibiting the dampness. However, it remains unclear which Coix seed compositions is responsible for these functions. Previous investigations have revealed that the main compositions of Coix seed are proteins, polysaccharides, oils and starches. The objectives of this study are to explore which is the most effective compositions in fortifying the spleen and examine how Coix seed works in regulating the water transport on the spleen deficiency and wet dampness (SDWD) rat model. MATERIALS AND METHODS: The rats used were divided into (i) control group, (ii) model group, (iii) decoction group, (iv) protein group, (v) polysaccharide group, (vi) oil group and (vii) starch group. The urine volume, the drinking volume and the water loading index in each group were calculated. Agilent 8*60K array was used for microarray-based gene expression analysis. The differential mRNAs related to the transport activity were screened. qRT-PCR was used to validate the mRNA microarray. RESULTS: The results demonstrated that all treatment groups could decrease the dampness of SDWD rats. mRNA microarray had significant effect on the protein group and the polysaccharide group in regulating the water transport, among which the most significant mRNA was Fabp6, Slc51a, Slc51b, Slc11a2, Slc4a10 and AQP3 respectively. CONCLUSION: The compositions of proteins and polysaccharides had the most significant effect in regulating the water transport of SDWD rat model. The contributing mRNA focused on Fabp, Slc and AQP family. [Mh] Termos MeSH primário: Coix/química Medicamentos de Ervas Chinesas/administração & dosagem Baço/efeitos dos fármacos Esplenopatias/tratamento farmacológico [Mh] Termos MeSH secundário: Animais Aquaporina 3/genética Aquaporina 3/metabolismo Proteínas de Ligação a Ácido Graxo/genética Proteínas de Ligação a Ácido Graxo/metabolismo Feminino Hormônios Gastrointestinais/genética Hormônios Gastrointestinais/metabolismo Seres Humanos Masculino Ratos Ratos Wistar Receptores Acoplados a Proteínas-G/genética Receptores Acoplados a Proteínas-G/metabolismo Sementes/química Baço/metabolismo Baço/fisiopatologia Esplenopatias/genética Esplenopatias/metabolismo Esplenopatias/fisiopatologia Água/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Aqp3 protein, rat); 0 (Drugs, Chinese Herbal); 0 (Fatty Acid-Binding Proteins); 0 (Gastrointestinal Hormones); 0 (Receptors, G-Protein-Coupled); 059QF0KO0R (Water); 117849-44-2 (fatty acid-binding protein 6); 158801-98-0 (Aquaporin 3) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170817 [Lr] Data última revisão: 170817 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170623 [St] Status: MEDLINE [do] DOI: 10.21010/ajtcam.v14i4.26

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[PMID]: 28372179 [Au] Autor: Xu L; Chen L; Ali B; Yang N; Chen Y; Wu F; Jin Z; Xu X [Ad] Endereço: State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, China. Electronic address: dapangxulei@163.com. [Ti] Título: Impact of germination on nutritional and physicochemical properties of adlay seed (Coixlachryma-jobi L.). [So] Source: Food Chem;229:312-318, 2017 Aug 15. [Is] ISSN: 0308-8146 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Adlay has garnered a great deal of research attentions in recent years as a highly nutritious food material and herbal medicine. This study characterized the changes of nutritional and physicochemical properties of adlay seeds during a 60-h germination. The results showed that the 60-h germination brought about a 3.4-fold increase in γ-aminobutyric acid (GABA) and 3.6-fold increase in coixol compared to ungerminated adlay seeds, while the triolein content slightly decreased. Some high molecular proteins were hydrolyzed into smaller proteins, peptides and amino acids after germination. Scanning electron micrographs (SEM) showed that the germination process destroyed the continuous matrix structure of adlay flour and created pits and holes on the surface of some starch granules. Germination resulted to changes in the pasting and gelatinization properties of adlay flour. The results of present study suggest that germination efficiently enhances the nutritional compounds while altering the physicochemical characteristics of adlay seeds. [Mh] Termos MeSH primário: Coix/química Germinação Sementes/química [Mh] Termos MeSH secundário: Fenômenos Químicos [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170505 [Lr] Data última revisão: 170505 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170405 [St] Status: MEDLINE

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[PMID]: 28352174 [Au] Autor: Qu D; Liu M; Huang M; Wang L; Chen Y; Liu C; Liu Y [Ad] Endereço: Research Center for Multicomponent Traditional Medicine and Microecology, Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine; Research Center for Multicomponent Traditional Medicine and Microecology, Jiangsu Provincial Academy of Tradit [Ti] Título: Octanoyl galactose ester-modified microemulsion system self-assembled by coix seed components to enhance tumor targeting and hepatoma therapy. [So] Source: Int J Nanomedicine;12:2045-2059, 2017. [Is] ISSN: 1178-2013 [Cp] País de publicação: New Zealand [La] Idioma: eng [Ab] Resumo: A nanosized drug delivery platform with a combination of rational components and tumor targeting is significant for enhancement of anticancer therapy and reduction of side effects. In this study, we developed a octanoyl galactose ester-modified microemulsion system self-assembled by coix seed components (Gal(oct)-C-MEs), which improved the tumor accumulation through asialoglycoprotein receptor-mediated endocytosis and promoted the antitumor efficacy through multicomponent-mediated synergistic effect. Octanoyl galactose ester (Gal(oct)) with a yield of 82.3% was synthesized through a green enzymatic reaction and multidimensional characterization. Gal(oct)-C-MEs with a spherical shape had a small and uniform particle size (58.49±1.03 nm), narrow polydispersity index (0.09±0.01) and neutral surface charge (-5.82±0.57 mV). In the cellular uptake studies, the internalized Gal(oct)-C-ME was 2.28-fold higher relative to that of coix seed component-based microemulsions (C-MEs). The half-maximal inhibitory concentration of Gal(oct)-C-MEs against HepG2 cells was 46.5±2.4 µg/mL, which was notably higher than that of C-MEs. Importantly, the intratumor fluorescence of HepG2 xenograft-bearing nude mice treated with Cy5/Gal(oct)-C-MEs was 1.9-fold higher relative to treatment with Cy5/C-MEs. In the study of antitumor efficacy in vivo, HepG2 xenograft-bearing nude mice intragastrically administered Gal(oct)-C-MEs for 14 days exhibited the strongest inhibition of tumor growth and the lowest toxicity against liver and kidney among all the treatments. In summary, Gal(oct)-C-ME, as a highly effective and safe anticancer drug delivery system, showed promising potential for hepatoma therapy. [Mh] Termos MeSH primário: Antineoplásicos/administração & dosagem Coix/química Sistemas de Liberação de Medicamentos/métodos Emulsões/química Galactose/síntese química [Mh] Termos MeSH secundário: Animais Antineoplásicos/efeitos adversos Antineoplásicos/química Antineoplásicos/farmacocinética Carcinoma Hepatocelular/tratamento farmacológico Ésteres/química Galactose/química Células Hep G2/efeitos dos fármacos Seres Humanos Neoplasias Hepáticas/tratamento farmacológico Masculino Camundongos Endogâmicos BALB C Camundongos Nus Tamanho da Partícula Sementes Ensaios Antitumorais Modelo de Xenoenxerto [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents); 0 (Emulsions); 0 (Esters); X2RN3Q8DNE (Galactose) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170530 [Lr] Data última revisão: 170530 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170330 [St] Status: MEDLINE [do] DOI: 10.2147/IJN.S125293

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[PMID]: 28350158 [Au] Autor: Qu D; Wang L; Liu M; Shen S; Li T; Liu Y; Huang M; Liu C; Chen Y; Mo R [Ad] Endereço: Affiliated Hospital of Integrated Traditional Chinese and Western Medicine, Nanjing University of Chinese Medicine , Nanjing 210028, China. [Ti] Título: Oral Nanomedicine Based on Multicomponent Microemulsions for Drug-Resistant Breast Cancer Treatment. [So] Source: Biomacromolecules;18(4):1268-1280, 2017 Apr 10. [Is] ISSN: 1526-4602 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The aim of this study is to demonstrate the enhanced therapeutic efficacy of anticancer drugs on drug-resistant breast cancer using multicomponent microemulsions (ECG-MEs) as an oral delivery system. The etoposide-loaded ECG-MEs were composed of coix seed oil and ginsenoside Rh2 (G-Rh2), both of which possess not only the synergistic antitumor effect with etoposide, but also have excipient-like properties. Orally administrated ECG-MEs were demonstrated to be able to accumulate at the tumor site following crossing the intestines as intact vehicles into the blood circulation. The spatiotemporal controlled release characteristics of ECG-MEs brought about the efficient P-gp inhibition by the initially released G-Rh2 and the increased intracellular accumulation of the sequentially released etoposide. The combination antitumor activity of etoposide, G-Rh2 and coix seed oil using ECG-MEs was verified on the xenograft drug-resistant breast tumor mouse models. In addition, the safety evaluation studies indicated that treatment with ECG-MEs did not cause any significant toxicity in vivo. These findings suggest that ECG-MEs as an oral formulation may offer a promising strategy to treat the drug-resistant breast cancer. [Mh] Termos MeSH primário: Protocolos de Quimioterapia Combinada Antineoplásica/uso terapêutico Neoplasias da Mama/tratamento farmacológico Resistência a Medicamentos Antineoplásicos/efeitos dos fármacos Neoplasias Mamárias Experimentais/tratamento farmacológico Nanoestruturas/química [Mh] Termos MeSH secundário: Administração Oral Animais Protocolos de Quimioterapia Combinada Antineoplásica/administração & dosagem Protocolos de Quimioterapia Combinada Antineoplásica/química Protocolos de Quimioterapia Combinada Antineoplásica/farmacocinética Apoptose/efeitos dos fármacos Neoplasias da Mama/metabolismo Neoplasias da Mama/patologia Células CACO-2 Coix/química Liberação Controlada de Fármacos Sinergismo Farmacológico Emulsões Etoposídeo/administração & dosagem Etoposídeo/química Etoposídeo/farmacocinética Etoposídeo/uso terapêutico Excipientes Feminino Ginsenosídeos/administração & dosagem Ginsenosídeos/química Ginsenosídeos/farmacocinética Ginsenosídeos/uso terapêutico Seres Humanos Células MCF-7 Neoplasias Mamárias Experimentais/metabolismo Neoplasias Mamárias Experimentais/patologia Camundongos Endogâmicos ICR Camundongos Nus Óleos Vegetais/administração & dosagem Óleos Vegetais/química Óleos Vegetais/farmacocinética Óleos Vegetais/uso terapêutico Ratos Sprague-Dawley Sementes/química Ensaios Antitumorais Modelo de Xenoenxerto [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Emulsions); 0 (Excipients); 0 (Ginsenosides); 0 (Plant Oils); 6PLQ3CP4P3 (Etoposide); 78214-33-2 (ginsenoside Rh2) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171110 [Lr] Data última revisão: 171110 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170329 [St] Status: MEDLINE [do] DOI: 10.1021/acs.biomac.7b00011

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[PMID]: 28098801 [Au] Autor: Li B; Qiao L; Li L; Zhang Y; Li K; Wang L; Qiao Y [Ad] Endereço: Beijing University of Chinese Medicine, 6 South Zhonghuan Road, Wangjing, Chaoyang District, Beijing 100102, China. libinzyy@163.com. [Ti] Título: A Novel Antihypertensive Derived from Adlay (Coix larchryma-jobi L. var. ma-yuen Stapf) Glutelin. [So] Source: Molecules;22(1), 2017 Jan 13. [Is] ISSN: 1420-3049 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: Our previous studies have shown that glutelin pepsin hydrolysate can effectively inhibit angiotensin converting enzyme (ACE) activity in vitro. The main purpose of this study was to obtain potent anti-hypertensive peptides from glutelin. The glutelin hydrolysates (CGH) were prepared by pepsin catalysis and further separated by an ultrafitration (UF) system, gel filtration chromatography (GFC) and reversed-phase high performance liquid chromatography (RP-HPLC). As a result, the sub-fraction F5-3 had the highest ACE-inhibitory activity. Six ACE inhibitory peptides were identifiedusing nano-liquid chromatography coupled to tandem mass spectrometry. The most potent peptide GAAGGAF (IC = 14.19 µmol·L ) was finally obtained by further molecular simulation screening and a series of division and optimization. Single oral administration of synthesized GAAGGAF at 15 mg/kg body weight (BW) in spontaneously hypertensively rats (SHR) could reduce the systolic blood pressure (SBP) around 27.50 mmHg and blood pressure-lowering effect lasted for at least 8 h. The study demonstrated for the first time that the ACE inhibitory peptide GAAGGAF from glutelin has a significant antihypertensive effect, and it could be a good natural ingredient for pharmaceuticals against hypertension and the related diseases. [Mh] Termos MeSH primário: Inibidores da Enzima Conversora de Angiotensina/farmacologia Anti-Hipertensivos/farmacologia Coix/química Glutens/química Hipertensão/tratamento farmacológico Peptídeos/farmacologia Peptidil Dipeptidase A/metabolismo [Mh] Termos MeSH secundário: Sequência de Aminoácidos Inibidores da Enzima Conversora de Angiotensina/química Inibidores da Enzima Conversora de Angiotensina/isolamento & purificação Animais Anti-Hipertensivos/química Anti-Hipertensivos/isolamento & purificação Cromatografia Líquida de Alta Pressão/métodos Expressão Gênica Hidrólise Hipertensão/fisiopatologia Masculino Simulação de Acoplamento Molecular Simulação de Dinâmica Molecular Pepsina A/química Peptídeos/química Peptídeos/isolamento & purificação Extratos Vegetais/química Ratos Ratos Endogâmicos SHR Sementes/química Ultrafiltração [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Angiotensin-Converting Enzyme Inhibitors); 0 (Antihypertensive Agents); 0 (Peptides); 0 (Plant Extracts); 8002-80-0 (Glutens); EC 3.4.15.1 (ACE protein, human); EC 3.4.15.1 (Peptidyl-Dipeptidase A); EC 3.4.23.1 (Pepsin A) [Em] Mês de entrada: 1705 [Cu] Atualização por classe: 170515 [Lr] Data última revisão: 170515 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170119 [St] Status: MEDLINE

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[PMID]: 27922683 [Au] Autor: Luo C; Wang X; An C; Hwang CF; Miao W; Yang L; Xu M; Bai A; Deng S [Ad] Endereço: School of Food and Pharmacy, Zhejiang Ocean University, Zhoushan, Zhejiang 316022, P.R. China. [Ti] Título: Molecular inhibition mechanisms of cell migration and invasion by coix polysaccharides in A549 NSCLC cells via targeting S100A4. [So] Source: Mol Med Rep;15(1):309-316, 2017 Jan. [Is] ISSN: 1791-3004 [Cp] País de publicação: Greece [La] Idioma: eng [Ab] Resumo: S100 calcium binding protein A4 (S100A4) promotes extracellular signal transduction, intercellular adhesion, motility and mobility. Different extracts from Coix lachryma-jobi have been used for the treatment of various types of cancer in Asia. In our previous study, the polysaccharide fraction extact, CP1, induced cell apoptosis of non­small cell lung cancer cells. In the current study, CP1 inhibited migration and invasion of A549 cells in a scratch wound healing assay and matrigel invasion assay, respectively. Furthermore, reverse transcription­polymerase chain reaction and western blotting demonstrated that CP1 downregulated the gene and protein expression levels of S100A4. In silico docking analysis demonstrated that polysaccharides may not interfere with dimerization, whereas, the affinity of polysaccharides for an S100A4­NMIIA pocket was margnially greater than at the dimerization sites. Thus, CP1 inhibited A549 cell migration and invasion potentially via downregulation of S100A4, and may also interact with the binding site of S100A4­NMIIA, which indicated that CP1 has potential as an alternative cancer chemotherapeutic by targeting S100A4. [Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia Carcinoma Pulmonar de Células não Pequenas/tratamento farmacológico Movimento Celular/efeitos dos fármacos Coix/química Neoplasias Pulmonares/tratamento farmacológico Polissacarídeos/farmacologia Proteína A4 de Ligação a Cálcio da Família S100/metabolismo [Mh] Termos MeSH secundário: Células A549 Antineoplásicos Fitogênicos/química Carcinoma Pulmonar de Células não Pequenas/genética Carcinoma Pulmonar de Células não Pequenas/metabolismo Carcinoma Pulmonar de Células não Pequenas/patologia Linhagem Celular Tumoral Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos Seres Humanos Pulmão/efeitos dos fármacos Pulmão/metabolismo Pulmão/patologia Neoplasias Pulmonares/genética Neoplasias Pulmonares/metabolismo Neoplasias Pulmonares/patologia Simulação de Acoplamento Molecular Invasividade Neoplásica/genética Invasividade Neoplásica/patologia Invasividade Neoplásica/prevenção & controle Polissacarídeos/química Proteína A4 de Ligação a Cálcio da Família S100/análise Proteína A4 de Ligação a Cálcio da Família S100/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Polysaccharides); 0 (S100 Calcium-Binding Protein A4) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170330 [Lr] Data última revisão: 170330 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161207 [St] Status: MEDLINE [do] DOI: 10.3892/mmr.2016.5985

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[PMID]: 27983650 [Au] Autor: Qiao L; Li B; Chen Y; Li L; Chen X; Wang L; Lu F; Luo G; Li G; Zhang Y [Ad] Endereço: Key Laboratory of TCM Foundation and New Drug Research, School of Chinese Material Medica, University of Chinese Medicine, Beijing 100102, China. b20100222012@163.com. [Ti] Título: Discovery of Anti-Hypertensive Oligopeptides from Adlay Based on In Silico Proteolysis and Virtual Screening. [So] Source: Int J Mol Sci;17(12), 2016 Dec 14. [Is] ISSN: 1422-0067 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: Adlay ( L.) was the commonly used Traditional Chinese Medicine (TCM) with high content of seed storage protein. The hydrolyzed bioactive oligopeptides of adlay have been proven to be anti-hypertensive effective components. However, the structures and anti-hypertensive mechanism of bioactive oligopeptides from adlay were not clear. To discover the definite anti-hypertensive oligopeptides from adlay, in silico proteolysis and virtual screening were implemented to obtain potential oligopeptides, which were further identified by biochemistry assay and molecular dynamics simulation. In this paper, ten sequences of adlay prolamins were collected and in silico hydrolyzed to construct the oligopeptide library with 134 oligopeptides. This library was reverse screened by anti-hypertensive pharmacophore database, which was constructed by our research team and contained ten anti-hypertensive targets. Angiotensin-I converting enzyme (ACE) was identified as the main potential target for the anti-hypertensive activity of adlay oligopeptides. Three crystal structures of ACE were utilized for docking studies and 19 oligopeptides were finally identified with potential ACE inhibitory activity. According to mapping features and evaluation indexes of pharmacophore and docking, three oligopeptides were selected for biochemistry assay. An oligopeptide sequence, NPATY (IC = 61.88 ± 2.77 µM), was identified as the ACE inhibitor by reverse-phase high performance liquid chromatography (RP-HPLC) assay. Molecular dynamics simulation of NPATY was further utilized to analyze interactive bonds and key residues. ALA354 was identified as a key residue of ACE inhibitors. Hydrophobic effect of VAL518 and electrostatic effects of HIS383, HIS387, HIS513 and Zn were also regarded as playing a key role in inhibiting ACE activities. This study provides a research strategy to explore the pharmacological mechanism of Traditional Chinese Medicine (TCM) proteins based on in silico proteolysis and virtual screening, which could be beneficial to reveal the pharmacological action of TCM proteins and provide new lead compounds for peptides-based drug design. [Mh] Termos MeSH primário: Anti-Hipertensivos/farmacologia Coix/química Simulação por Computador Descoberta de Drogas Avaliação Pré-Clínica de Medicamentos Oligopeptídeos/farmacologia Proteólise Interface Usuário-Computador [Mh] Termos MeSH secundário: Inibidores da Enzima Conversora de Angiotensina/química Cromatografia Líquida de Alta Pressão Cromatografia de Fase Reversa Cristalografia por Raios X Bases de Dados de Proteínas Simulação de Acoplamento Molecular Biblioteca de Peptídeos Fatores de Tempo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Angiotensin-Converting Enzyme Inhibitors); 0 (Antihypertensive Agents); 0 (Oligopeptides); 0 (Peptide Library) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170403 [Lr] Data última revisão: 170403 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161217 [St] Status: MEDLINE

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[PMID]: 27801779 [Au] Autor: An TJ; Shin KS; Paul NC; Kim YG; Cha SW; Moon Y; Yu SH; Oh SK [Ad] Endereço: Department of Herbal Crop Research, National Institute of Horticultural and Herbal Science (NIHHS), Eumseong, Chungbuk 27709, Korea. atj0083@korea.kr. [Ti] Título: Prevalence, Characterization, and Mycotoxin Production Ability of Fusarium Species on Korean Adlay (Coix lacrymal-jobi L.) Seeds. [So] Source: Toxins (Basel);8(11), 2016 10 27. [Is] ISSN: 2072-6651 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: Adlay seed samples were collected from three adlay growing regions (Yeoncheon, Hwasun, and Eumseong region) in Korea during 2012. Among all the samples collected, 400 seeds were tested for fungal occurrence by standard blotter and test tube agar methods and different taxonomic groups of fungal genera were detected. The most predominant fungal genera encountered were , , , , , and . species accounted for 45.6% of all species found; and, with phylogenetic analysis based on the combined sequences of two protein coding genes (EF-1α and ß-tubulin), 10 species were characterized namely, (11.67%), (10.33%), (8.67%), (6.00%), (5.67%), (1.67%), (0.67%), (0.33%), (0.33%), and (0.33%). The species were then examined for their morphological characteristics to confirm their identity. Morphological observations of the species correlated well with and confirmed their molecular identification. The ability of these isolates to produce the mycotoxins fumonisin (FUM) and zearalenone (ZEN) was tested by the ELISA quantitative analysis method. The result revealed that FUM was produced only by and that ZEN was produced by and . [Mh] Termos MeSH primário: Coix/microbiologia Fumonisinas/metabolismo Fusarium/metabolismo Sementes/microbiologia Zearalenona/biossíntese [Mh] Termos MeSH secundário: Sequência de Bases DNA Fúngico/análise Fusarium/genética Fusarium/isolamento & purificação Filogenia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (DNA, Fungal); 0 (Fumonisins); 5W827M159J (Zearalenone) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171122 [Lr] Data última revisão: 171122 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161102 [St] Status: MEDLINE

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[PMID]: 27769819 [Au] Autor: Pang DR; Su XQ; Zhu ZX; Sun J; Li YT; Song YL; Zhao YF; Tu PF; Zheng J; Li J [Ad] Endereço: Modern Research Center for Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, People's Republic of China; School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 100102, People's Republic of China. [Ti] Título: Flavonoid dimers from the total phenolic extract of Chinese dragon's blood, the red resin of Dracaena cochinchinensis. [So] Source: Fitoterapia;115:135-141, 2016 Dec. [Is] ISSN: 1873-6971 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Eight new flavonoid dimers, named cochinchinenins I-M (1-5), including three pairs of enantiomers (1a/1b-3a/3b) and two optically pure flavonoid dimers (4-5), along with a known analogue (6), were isolated from total phenolic extract of the red resin of Dracaena cochinchinensis (Chinese dragon's blood). The planar structures of 1-5 were elucidated by extensive spectroscopic analysis including HRESIMS and 1D/2D NMR. Their absolute configurations were determined on the basis of experimental and calculated electronic circular dichroism (ECD) data. Compounds 4 and 5 exhibited significant inhibition of nitric oxide production in lipopolysaccharide-stimulated BV-2 microglial cells with IC value of 4.9±0.4 and 5.4±0.6µM, respectively. [Mh] Termos MeSH primário: Dracaena/química Flavonoides/química Extratos Vegetais/química Resinas Vegetais/química [Mh] Termos MeSH secundário: Animais Linhagem Celular Coix/química Flavonoides/isolamento & purificação Camundongos Microglia/efeitos dos fármacos Estrutura Molecular Óxido Nítrico/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Flavonoids); 0 (Plant Extracts); 0 (Resins, Plant); 31C4KY9ESH (Nitric Oxide); M3YJ2C28IC (dragon's blood) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161107 [St] Status: MEDLINE

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[PMID]: 27459907 [Au] Autor: Qian Y; Yang B; Xiong Y; Gu M [Ad] Endereço: Pharmaceutics Preparation Center, Zhejiang Hospital of Traditional Chinese Medicine, Hangzhou, Zhejiang 310006, P.R. China. [Ti] Título: Coix seed emulsion synergistically enhances the antitumor activity of gemcitabine in pancreatic cancer through abrogation of NF-κB signaling. [So] Source: Oncol Rep;36(3):1517-25, 2016 Sep. [Is] ISSN: 1791-2431 [Cp] País de publicação: Greece [La] Idioma: eng [Ab] Resumo: Clinical outcomes in patients with pancreatic cancer (PC) continue to be dismal, in part due to de novo and acquired chemoresistance. In the present study, we provide preclinical evidence that pre-treatment with coix seed emulsion, an injectable agent extracted from coix seeds, synergistically sensitized PC cell lines (BxPC-3, PANC-1 and AsPC-1) to gemcitabine, both in vitro and in vivo. Such pretreatment led to significant induction of pro-apoptosis proteins, including caspase-3, cleaved-PARP and Bax (P<0.05), after lower doses of gemcitabine compared to monotherapy. We also showed that coix seed emulsion suppressed the constitutive and gemcitabine-induced activation of nuclear factor-κB (NF-κB), as shown with the use of electrophoretic mobility shift, reporter and immunoblotting analyses. Coix seed emulsion pretreatment also downregulated the NF-κB-dependent anti­apoptotic molecules Bcl-2, survivin and cyclooxygenase-2. In vivo, coix seed emulsion combined with gemcitabine had a much greater antitumor effect than the effect of either agent alone, consistent with the downregulation of the proliferation index, and the results of immunostaining for Ki-67, or for the NF-κB subunit p65. Overall, our data demonstrated that coix seed emulsion abrogated gemcitabine-induced activation of NF-κB, and synergistically sensitized PC cells to gemcitabine therapy. [Mh] Termos MeSH primário: Coix/química Desoxicitidina/análogos & derivados Medicamentos de Ervas Chinesas/farmacologia Emulsões/farmacologia NF-kappa B/metabolismo Neoplasias Pancreáticas/tratamento farmacológico Sementes/química [Mh] Termos MeSH secundário: Animais Antineoplásicos/farmacologia Apoptose/efeitos dos fármacos Linhagem Celular Tumoral Proliferação Celular/efeitos dos fármacos Desoxicitidina/farmacologia Regulação para Baixo/efeitos dos fármacos Resistência a Medicamentos Antineoplásicos/efeitos dos fármacos Sinergismo Farmacológico Seres Humanos Masculino Camundongos Camundongos Endogâmicos BALB C Camundongos Nus Neoplasias Pancreáticas/metabolismo Transdução de Sinais/efeitos dos fármacos Ensaios Antitumorais Modelo de Xenoenxerto/métodos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents); 0 (Drugs, Chinese Herbal); 0 (Emulsions); 0 (NF-kappa B); 0W860991D6 (Deoxycytidine); B76N6SBZ8R (gemcitabine) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170309 [Lr] Data última revisão: 170309 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160728 [St] Status: MEDLINE [do] DOI: 10.3892/or.2016.4958

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