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[PMID]:29176773
[Au] Autor:Volterrani M; Minelli A; Gaetani M; Grossi N; Magni S; Caturegli L
[Ad] Endereço:Department of Agriculture, Food and Environment, University of Pisa, Pisa, Italy.
[Ti] Título:Reflectance, absorbance and transmittance spectra of bermudagrass and manilagrass turfgrass canopies.
[So] Source:PLoS One;12(11):e0188080, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Leaves act as a primary organ for the interception of solar radiation and their spatial arrangement determines how the plant canopy interacts with light. Many studies have been carried out on the penetration of radiation into crops however to date, few results are available on turfgrasses, mainly due to the difficulties of introducing sensors into the turf without disturbing the natural position of the leaves. In the present research two warm season turfgrasses, hybrid bermudagrass (Cynodon dactylon × transvaalensis) 'Patriot' and manilagrass (Zoysia matrella) 'Zeon', were studied. The aim was to describe their canopy architecture grown with minimal disturbance to the natural arrangement of the leaves and stems, and to determine the potential effects of canopy architecture on light penetration and reflectance. Radiometric measurements were carried out at eight different profile levels of turfgrasses that were up to 12 cm tall. A LI-COR 1800 spectroradiometer with an optical fiber cable and a 7 mm diameter sensor was used. Measurements were carried out in the 390-1100 nm region at 5 nm intervals. The LAI value was higher for the manilagrass (9.0) than for the hybrid bermudagrass (5.6). The transmitted radiation was found to be closely dependent on downward cumulative LAI. Despite a more upright habit (mean insertion angle of 22.4° ±3.4), Zoysia matrella showed a higher NIR reflectance compared to Cdxt, which has a horizontal leaf arrangement (mean insertion angle 62.1° ± 9.6). The species studied showed substantial differences both in terms of phytometric characteristics and in the capacity to attenuate solar radiation.
[Mh] Termos MeSH primário: Cynodon/fisiologia
Luz
Poaceae/fisiologia
[Mh] Termos MeSH secundário: Biomassa
Folhas de Planta/fisiologia
Folhas de Planta/efeitos da radiação
Brotos de Planta/fisiologia
Chuvas
Análise Espectral
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171219
[Lr] Data última revisão:
171219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188080


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[PMID]:28881947
[Au] Autor:Murphey Coy R; Held DW; Kloepper JW
[Ad] Endereço:Department of Entomology and Plant Pathology, Auburn University, 301 Funchess Hall, Auburn, AL 36849.
[Ti] Título:Bacterial Inoculant Treatment of Bermudagrass Alters Ovipositional Behavior, Larval and Pupal Weights of the Fall Armyworm (Lepidoptera: Noctuidae).
[So] Source:Environ Entomol;46(4):831-838, 2017 08 01.
[Is] ISSN:1938-2936
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nonpathogenic soil bacteria can colonize the rhizosphere and induce unique plant phenotypes that may influence plant-insect interactions. However, few studies have considered the influences of bacteria-plant interactions on insect feeding and oviposition. The objective of this study was to determine how rhizobacterial inoculation of bermudagrass affects larval development and ovipositional behaviors of the fall armyworm (Spodoptera frugiperda J.E. Smith). Eight blends of rhizobacteria known to induce root or shoot growth in grasses were applied weekly to hybrid bermudagrass for 5 wk. Oviposition was evaluated in two no-choice trials with bacteria-treated, fertilized, or nontreated grass. Grass blades from these treatments were extracted in polar and nonpolar solvents and assayed for oviposition responses. Another experiment compared the development of fall armyworm larvae on bermudagrass treated with each of the eight rhizobacterial blends for 5 wk to larvae fed nontreated bermudagrass. Females deposited more eggs on nontreated and fertilized grass and ≤34% of eggs on grass treated with rhizobacterial blends. Moths exposed to polar and nonpolar extracts were unable to reproduce these results. Larval and pupal weights at days 10 and 12 and the number of adults to eclose were lower for larvae fed some, but not all, bacteria-treated bermudagrass relative to controls. This is one of the few studies to investigate plant-microbe-insect interactions in an economically important system. Although the effects noted with fall armyworm are limited, induced changes in roots also reported for these bacteria may have greater utility than foliar changes for mediating interactions with biotic or abiotic stresses.
[Mh] Termos MeSH primário: Inoculantes Agrícolas/química
Bacillales/química
Cynodon/microbiologia
Mariposas/microbiologia
Mariposas/fisiologia
Controle Biológico de Vetores
[Mh] Termos MeSH secundário: Animais
Bacillus/química
Brevibacillus/química
Cynodon/crescimento & desenvolvimento
Larva/crescimento & desenvolvimento
Larva/microbiologia
Larva/fisiologia
Mariposas/crescimento & desenvolvimento
Oviposição
Paenibacillus/química
Pupa/crescimento & desenvolvimento
Pupa/microbiologia
Pupa/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1093/ee/nvx102


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[PMID]:28617867
[Au] Autor:Huang YY; Cho ST; Haryono M; Kuo CH
[Ad] Endereço:Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
[Ti] Título:Complete chloroplast genome sequence of common bermudagrass (Cynodon dactylon (L.) Pers.) and comparative analysis within the family Poaceae.
[So] Source:PLoS One;12(6):e0179055, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Common bermudagrass (Cynodon dactylon (L.) Pers.) belongs to the subfamily Chloridoideae of the Poaceae family, one of the most important plant families ecologically and economically. This grass has a long connection with human culture but its systematics is relatively understudied. In this study, we sequenced and investigated the chloroplast genome of common bermudagrass, which is 134,297 bp in length with two single copy regions (LSC: 79,732 bp; SSC: 12,521 bp) and a pair of inverted repeat (IR) regions (21,022 bp). The annotation contains a total of 128 predicted genes, including 82 protein-coding, 38 tRNA, and 8 rRNA genes. Additionally, our in silico analyses identified 10 sets of repeats longer than 20 bp and predicted the presence of 36 RNA editing sites. Overall, the chloroplast genome of common bermudagrass resembles those from other Poaceae lineages. Compared to most angiosperms, the accD gene and the introns of both clpP and rpoC1 genes are missing. Additionally, the ycf1, ycf2, ycf15, and ycf68 genes are pseudogenized and two genome rearrangements exist. Our phylogenetic analysis based on 47 chloroplast protein-coding genes supported the placement of common bermudagrass within Chloridoideae. Our phylogenetic character mapping based on the parsimony principle further indicated that the loss of the accD gene and clpP introns, the pseudogenization of four ycf genes, and the two rearrangements occurred only once after the most recent common ancestor of the Poaceae diverged from other monocots, which could explain the unusual long branch leading to the Poaceae when phylogeny is inferred based on chloroplast sequences.
[Mh] Termos MeSH primário: Cloroplastos/genética
Cynodon/genética
Genoma de Cloroplastos
Poaceae/genética
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Mapeamento Cromossômico
Evolução Molecular
Ordem dos Genes
Tamanho do Genoma
Filogenia
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179055


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[PMID]:28493962
[Au] Autor:Zheng Y; Xu S; Liu J; Zhao Y; Liu J
[Ad] Endereço:College of Forestry, Henan University of Science and Technology, Luoyang, Henan, P. R. of China.
[Ti] Título:Genetic diversity and population structure of Chinese natural bermudagrass [Cynodon dactylon (L.) Pers.] germplasm based on SRAP markers.
[So] Source:PLoS One;12(5):e0177508, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Bermudagrass [Cynodon dactylon (L.) Pers.], an important turfgrass used in public parks, home lawns, golf courses and sports fields, is widely distributed in China. In the present study, sequence-related amplified polymorphism (SRAP) markers were used to assess genetic diversity and population structure among 157 indigenous bermudagrass genotypes from 20 provinces in China. The application of 26 SRAP primer pairs produced 340 bands, of which 328 (96.58%) were polymorphic. The polymorphic information content (PIC) ranged from 0.36 to 0.49 with a mean of 0.44. Genetic distance coefficients among accessions ranged from 0.04 to 0.61, with an average of 0.32. The results of STRUCTURE analysis suggested that 157 bermudagrass accessions can be grouped into three subpopulations. Moreover, according to clustering based on the unweighted pair-group method of arithmetic averages (UPGMA), accessions were divided into three major clusters. The UPGMA dendrogram revealed that accessions from identical or adjacent areas were generally, but not entirely, clustered into the same cluster. Comparison of the UPGMA dendrogram and the Bayesian STRUCTURE analysis showed general agreement between the population subdivisions and the genetic relationships among accessions. Principal coordinate analysis (PCoA) with SRAP markers revealed a similar grouping of accessions to the UPGMA dendrogram and STRUCTUE analysis. Analysis of molecular variance (AMOVA) indicated that 18% of total molecular variance was attributed to diversity among subpopulations, while 82% of variance was associated with differences within subpopulations. Our study represents the most comprehensive investigation of the genetic diversity and population structure of bermudagrass in China to date, and provides valuable information for the germplasm collection, genetic improvement, and systematic utilization of bermudagrass.
[Mh] Termos MeSH primário: Cynodon/genética
Polimorfismo Genético
Sementes/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Análise por Conglomerados
Ecótipo
Marcadores Genéticos
Genética Populacional
Filogenia
Análise de Componente Principal
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177508


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[PMID]:28273510
[Au] Autor:Li X; Gitau MM; Han S; Fu J; Xie Y
[Ad] Endereço:Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Science, Wuhan 430074, China; University of Chinese Academy of Sciences, Beijing, China.
[Ti] Título:Effects of cadmium-resistant fungi Aspergillus aculeatus on metabolic profiles of bermudagrass [Cynodondactylon (L.)Pers.] under Cd stress.
[So] Source:Plant Physiol Biochem;114:38-50, 2017 May.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Plants' tolerance to heavy metal stress may be induced by the exploitation of microbes. The objectives of this study were to investigate the effect of cadmium (Cd)-resistant fungus, Aspergillus aculeatus, on tolerance to Cd and alteration of metabolites in bermudagrass under Cd stress, and identify the predominant metabolites associated with Cd tolerance. Two genotypes of bermudagrass with contrasting Cd tolerance (Cd-sensitive 'WB92' and Cd-tolerant 'WB242') were exposed to 0, 50, 150 and 250 mg kg Cd for 21 days. Physiological responses of bermudagrass to Cd stress were evaluated based on the relative growth rate (RGR) and normalized relative transpiration rate (NRT). Plants inoculated with A. aculeatus exhibited higher RGR and NRT under Cd stress than those of non-inoculated plants, regardless of genotypes. A total of 32 Cd-responsive metabolites in leaves and 21 in roots were identified in the two genotypes, including organic acids, amino acids, sugars, and fatty acids and others. Interestingly, under Cd stress, the leaves of inoculated 'WB92' accumulated less citric acid, aspartic acid, glutamic acid, sucrose, galactose, but more sorbose and glucose, while inoculated 'WB242' leaves had less citric acid, malic acid, sucrose, sorbose, but more fructose and glucose, compared to non-inoculated plants. In 'WB92' roots, the A. aculeatus reduced mannose content, but increased trehalose and citric acid content, while in 'WB242', it decreased sucrose, but enhanced citric acid content, compared to Cd regime. The results of this study suggest that A. aculeatus may induce accumulation of different metabolites associated with Cd tolerance in bermudagrass.
[Mh] Termos MeSH primário: Aspergillus/fisiologia
Cádmio/toxicidade
Cynodon/efeitos dos fármacos
Cynodon/microbiologia
[Mh] Termos MeSH secundário: Aspergillus/efeitos dos fármacos
Cádmio/farmacocinética
Análise por Conglomerados
Cynodon/metabolismo
Inativação Metabólica
Folhas de Planta/efeitos dos fármacos
Folhas de Planta/metabolismo
Raízes de Plantas/efeitos dos fármacos
Raízes de Plantas/metabolismo
Análise de Componente Principal
Estresse Fisiológico/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
00BH33GNGH (Cadmium)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170620
[Lr] Data última revisão:
170620
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE


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[PMID]:28177397
[Au] Autor:Thomas MV; Hersom MJ; Thrift TA; Yelich JV
[Ti] Título:Comparison of wet brewers' grains or dried distillers' grains as supplements to conserved bermudagrass forage as winter feeding options for beef cows.
[So] Source:J Anim Sci;95(1):361-370, 2017 Jan.
[Is] ISSN:1525-3163
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to examine the use of 2 byproduct supplements and conserved warm-season forage as winter feeding options for primiparous beef cows. Gestating Angus ( = 48) and Brangus ( = 24) 2-yr-old cows were stratified by BW and breed to 1 of 12 pens. Pens were randomly assigned 1 of 2 supplements, wet brewers' grains (WBG) or dried distillers' grains (DDG). Coastal bermudagrass hay or round bale silage (RBS) was fed free choice (6 pens each) and cows received WBG or DDG supplements at a daily rate of 0.05% BW (DM basis) prorated for feeding 3 d/wk. Total BW and BCS changes did not differ ( = 0.65 and = 0.93, respectively) between DDG- and WBG-supplemented cows. Total amount of forage DM offered and mean calculated daily forage DM offered did not differ ( = 0.59 and = 0.20, respectively) between supplement treatments. Estimated daily mean and total supplement DM offered was greater ( < 0.001) for WBG than for DDG treatments. The same 2 supplements and forage sources were used in an unbalanced 6 × 4 design to measure intake, digestibility, and rumen parameters in ruminally fistulated steers. Supplement did not affect forage DMI of hay ( = 0.31) or RBS ( = 0.63). Total DMI was not different ( = 0.37 and = 0.73) for hay-based and RBS-based diets, respectively. Total tract digestibility tended to be greater ( = 0.06) for DDG than for WBG in hay diets but was not different ( = 0.76) for RBS diets. Daily mean ruminal pH was greater ( = 0.03) for WBG than for DDG when supplemented to hay-based diets. In RBS diets, a supplement × hour interaction ( = 0.05) existed for ruminal pH. Daily mean ruminal ammonia N concentration was greater ( < 0.001) for WBG-supplemented diets compared with DDG-supplemented diets. A supplement × hour interaction existed for ruminal ammonia in hay- ( < 0.001) and RBS- ( = 0.09) based diets. Ruminal pH and ammonia N concentrations stayed in ranges adequate to support ruminal metabolism and cattle performance. Marginal differences in ruminal measures between high-moisture and dry byproduct supplements resulted in no differences in cow performance when offered with hay or high-moisture forage. High-moisture forage sources can be coupled with high-moisture byproduct supplements.
[Mh] Termos MeSH primário: Bovinos/fisiologia
Cynodon
Dieta/veterinária
Suplementos Nutricionais
Silagem/análise
[Mh] Termos MeSH secundário: Amônia/metabolismo
Animais
Digestão
Feminino
Rúmen/metabolismo
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE; RANDOMIZED CONTROLLED TRIAL
[Nm] Nome de substância:
7664-41-7 (Ammonia)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170412
[Lr] Data última revisão:
170412
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.2527/jas.2016.0781


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[PMID]:28177352
[Au] Autor:Ranches J; Vendramini JM; Arthington JD
[Ti] Título:Effects of selenium biofortification of hayfields on measures of selenium status in cows and calves consuming these forages.
[So] Source:J Anim Sci;95(1):120-128, 2017 Jan.
[Is] ISSN:1525-3163
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Two experiments were conducted to evaluate the Se status of weaned calves (Exp. 1) and pregnant cows and newborn calves (Exp. 2) consuming bermudagrass [ (L.) Pers.] fertilized with Se. Sodium selenate was dissolved into water (8.8 g/L) and sprayed onto hayfields. Selenium-fertilized forage had greater ( ≤ 0.001) Se concentration compared with the control forage without Se fertilization (7.7 ± 1.81 vs. 0.1 ± 0.04 mg Se/kg DM and 10.8 vs. 0.1 mg Se/kg DM for Exp. 1 and Exp. 2, respectively). In Exp. 1, beef calves ( = 32; 176 ± 8.7 kg initial BW) were stratified by BW and randomly assigned to pens (16 pens; 2 calves/pen). Treatments were randomly assigned to pens, including control (no supplemental Se), Se hay, or sodium selenite ( = 2, 7, and 7 pens, respectively). A 42-d pair-feeding design was used, wherein each pen receiving Se hay was paired to a pen receiving sodium selenite. Blood and liver samples were collected on d 0, 21, and 42. Liver Se concentrations were greatest ( ≤ 0.005) on d 42 for calves provided Se hay compared with calves provided sodium selenite or control. This difference was attributed only to paired-feeding groups consuming <3 mg Se daily. In Exp. 2, mature, late-pregnancy cows were randomly assigned to 3 treatments: Se hay ( = 12), sodium selenite ( = 9), or control (no supplemental Se; = 6). Cows assigned to the sodium selenite and Se hay treatments were provided 2.5 mg of supplemental Se daily. Upon enrollment, cows were moved into individual feeding areas at an estimated 30 d prior to calving. Following calving, cotyledon and colostrum samples were collected from cows. Four days later, liver and blood samples were collected from both cows and calves. Selenium-supplemented cows had greater ( ≤ 0.001) liver, cotyledon, plasma, and whole blood Se concentrations compared with cows not receiving supplemental Se. Furthermore, cows provided Se hay tended ( = 0.11) to have greater liver Se concentrations compared with cows provided sodium selenite. Calves born to Se-supplemented cows had greater ( = 0.001) plasma Se concentrations than calves born to cows receiving no supplemental Se. Furthermore, calves born to cows provided Se hay tended ( = 0.06) to have greater plasma Se concentrations compared with calves born to cows provided sodium selenite. These data imply that Se biofortification of hayfields is an effective method to increase Se concentration of forage. Consumption of these forages result in increased Se status of weaned calves, periparturient cows, and their calves.
[Mh] Termos MeSH primário: Ração Animal
Biofortificação
Bovinos/fisiologia
Cynodon/metabolismo
Selênio/metabolismo
Selenito de Sódio/metabolismo
[Mh] Termos MeSH secundário: Fenômenos Fisiológicos da Nutrição Animal
Animais
Cynodon/química
Dieta/veterinária
Suplementos Nutricionais
Feminino
Gravidez
Selênio/sangue
Selenito de Sódio/química
Desmame
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
H6241UJ22B (Selenium); HIW548RQ3W (Sodium Selenite)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170209
[St] Status:MEDLINE
[do] DOI:10.2527/jas.2016.0943


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[PMID]:28168408
[Au] Autor:Khanal S; Kim C; Auckland SA; Rainville LK; Adhikari J; Schwartz BM; Paterson AH
[Ad] Endereço:Plant Genome Mapping Laboratory, University of Georgia, Athens, GA, 30602, USA.
[Ti] Título:SSR-enriched genetic linkage maps of bermudagrass (Cynodon dactylon × transvaalensis), and their comparison with allied plant genomes.
[So] Source:Theor Appl Genet;130(4):819-839, 2017 Apr.
[Is] ISSN:1432-2242
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: We report SSR-enriched genetic maps of bermudagrass that: (1) reveal partial residual polysomic inheritance in the tetraploid species, and (2) provide insights into the evolution of chloridoid genomes. This study describes genetic linkage maps of two bermudagrass species, Cynodon dactylon (T89) and Cynodon transvaalensis (T574), that integrate heterologous microsatellite markers from sugarcane into frameworks built with single-dose restriction fragments (SDRFs). A maximum likelihood approach was used to construct two separate parental maps from a population of 110 F progeny of a cross between the two parents. The T89 map is based on 291 loci on 34 cosegregating groups (CGs), with an average marker spacing of 12.5 cM. The T574 map is based on 125 loci on 14 CGs, with an average marker spacing of 10.7 cM. Six T89 and one T574 CG(s) deviated from disomic inheritance. Furthermore, marker segregation data and linkage phase analysis revealed partial residual polysomic inheritance in T89, suggesting that common bermudagrass is undergoing diploidization following whole genome duplication (WGD). Twenty-six T89 CGs were coalesced into 9 homo(eo)logous linkage groups (LGs), while 12 T574 CGs were assembled into 9 LGs, both putatively representing the basic chromosome complement (x = 9) of the species. Eight T89 and two T574 CGs remain unassigned. The marker composition of bermudagrass ancestral chromosomes was inferred by aligning T89 and T574 homologs, and used in comparisons to sorghum and rice genome sequences based on 108 and 91 significant blast hits, respectively. Two nested chromosome fusions (NCFs) shared by two other chloridoids (i.e., zoysiagrass and finger millet) and at least three independent translocation events were evident during chromosome number reduction from 14 in the polyploid common ancestor of Poaceae to 9 in Cynodon.
[Mh] Termos MeSH primário: Mapeamento Cromossômico
Cynodon/genética
Ligação Genética
Genoma de Planta
[Mh] Termos MeSH secundário: Cromossomos de Plantas
DNA de Plantas/genética
Etiquetas de Sequências Expressas
Padrões de Herança
Funções Verossimilhança
Repetições de Microssatélites
Polimorfismo de Fragmento de Restrição
Tetraploidia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170917
[Lr] Data última revisão:
170917
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170208
[St] Status:MEDLINE
[do] DOI:10.1007/s00122-017-2854-z


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[PMID]:27720768
[Au] Autor:Rahmatpour S; Khan AH; Nasiri Kalmarzi R; Rajabibazl M; Tavoosidana G; Motevaseli E; Zarghami N; Sadroddiny E
[Ad] Endereço:Department of Medical Biotechnology, Faculty of Advanced Medical Sciences, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
[Ti] Título:Application of immuno-PCR assay for the detection of serum IgE specific to Bermuda allergen.
[So] Source:Mol Cell Probes;32:1-4, 2017 Apr.
[Is] ISSN:1096-1194
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In vivo and in vitro tests are the two major ways of identifying the triggering allergens in sensitized individuals with allergic symptoms. Both methods are equally significant in terms of sensitivity and specificity. However, in certain circumstances, in vitro methods are highly preferred because they circumvent the use of sensitizing drugs in patients. In current study, we described a highly sensitive immuno-PCR (iPCR) assay for serum IgE specific to Bermuda allergens. Using oligonucleotide-labelled antibody, we used iPCR for the sensitive detection of serum IgE. The nucleotide sequence was amplified using conventional PCR and the bands were visualized on 2.5% agarose gel. Results demonstrated a 100-fold enhancement in sensitivity of iPCR over commercially available enzyme-linked immunosorbent assay (ELISA) kit. Our iPCR method was highly sensitive for Bermuda-specific serum IgE and could be beneficial in allergy clinics.
[Mh] Termos MeSH primário: Alérgenos/imunologia
Especificidade de Anticorpos/imunologia
Cynodon/imunologia
Imunoglobulina E/sangue
Reação em Cadeia da Polimerase/métodos
[Mh] Termos MeSH secundário: Seres Humanos
Sensibilidade e Especificidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 37341-29-0 (Immunoglobulin E)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161011
[St] Status:MEDLINE


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[PMID]:27457694
[Au] Autor:Biswas TK; Pandit S; Chakrabarti S; Banerjee S; Poyra N; Seal T
[Ad] Endereço:Research Unit, J.B. Roy State Ayurvedic Medical College & Hospital, 170-172, Raja Dinendra Street, Kolkata 700004, India. Electronic address: biswastuhin@rediffmail.com.
[Ti] Título:Evaluation of Cynodon dactylon for wound healing activity.
[So] Source:J Ethnopharmacol;197:128-137, 2017 Feb 02.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND AIMS: Research in the field of wound healing is very recent. The concept of wound healing is changing from day to day. Ayurveda is the richest source of plant drugs for management of wounds and Cynodon dactylon L. is one such. The plant is used as hemostatic and wound healing agent from ethnopharmacological point of view. Aim of the present study is scientific validation of the plant for wound healing activity in detail. MATERIALS AND METHODS: Aqueous extract of the plant was prepared and phytochemical constituents were detected by HPLC analysis. Acute and dermatological toxicity study of the extract was performed. Pharmacological testing of 15% ointment (w/w) of the extract with respect to placebo control and standard comparator framycetin were done on full thickness punch wound in Wister rats and effects were evaluated based on parameters like wound contraction size (mm ), tensile strength (g); tissue DNA, RNA, protein, hydroxyproline and histological examination. The ointment was applied on selected clinical cases of chronic and complicated wounds and efficacy was evaluated on basis of scoring on granulation, epithelialization, vascularity as well as routine hematological investigations. RESULTS: Significant results (p<0.05) were observed both in pharmacological and clinical studies. CONCLUSION: The present research with aqueous extract of Cynodon dactylon explores its potential wound healing activity in animal model and subsequent feasibility in human subjects. Phenolic acids and flavonoids present in c. dactylon supports its wound healing property for its anti-oxidative activity that are responsible for collagenesis.
[Mh] Termos MeSH primário: Cynodon/química
Extratos Vegetais/farmacologia
Cicatrização/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Fármacos Dermatológicos/química
Fármacos Dermatológicos/farmacologia
Feminino
Masculino
Camundongos
Pomadas/química
Pomadas/farmacologia
Fitoterapia/métodos
Extratos Vegetais/química
Ratos
Ratos Wistar
Pele/efeitos dos fármacos
Resistência à Tração/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dermatologic Agents); 0 (Ointments); 0 (Plant Extracts)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170608
[Lr] Data última revisão:
170608
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160727
[St] Status:MEDLINE



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