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  1 / 473 MEDLINE  
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[PMID]:29342197
[Au] Autor:Talukder SK; Azhaguvel P; Chekhovskiy K; Saha MC
[Ad] Endereço:Noble Research Institute, LLC, Ardmore, OK, United States of America.
[Ti] Título:Molecular discrimination of tall fescue morphotypes in association with Festuca relatives.
[So] Source:PLoS One;13(1):e0191343, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tall fescue (Festuca arundinacea Schreb.) is an important cool-season perennial grass species used as forage and turf, and in conservation plantings. There are three morphotypes in hexaploid tall fescue: Continental, Mediterranean and Rhizomatous. This study was conducted to develop morphotype-specific molecular markers to distinguish Continental and Mediterranean tall fescues, and establish their relationships with other species of the Festuca genus for genomic inference. Chloroplast sequence variation and simple sequence repeat (SSR) polymorphism were explored in 12 genotypes of three tall fescue morphotypes and four Festuca species. Hypervariable chloroplast regions were retrieved by using 33 specifically designed primers followed by sequencing the PCR products. SSR polymorphism was studied using 144 tall fescue SSR primers. Four chloroplast (NFTCHL17, NFTCHL43, NFTCHL45 and NFTCHL48) and three SSR (nffa090, nffa204 and nffa338) markers were identified which can distinctly differentiate Continental and Mediterranean morphotypes. A primer pair, NFTCHL45, amplified a 47 bp deletion between the two morphotypes is being routinely used in the Noble Research Institute's core facility for morphotype discrimination. Both chloroplast sequence variation and SSR diversity showed a close association between Rhizomatous and Continental morphotypes, while the Mediterranean morphotype was in a distant clade. F. pratensis and F. arundinacea var. glaucescens, the P and G1G2 genome donors, respectively, were grouped with the Continental clade, and F. mairei (M1M2 genome) grouped with the Mediterranean clade in chloroplast sequence variation, while both F. pratensis and F. mairei formed independent clade in SSR analysis. Age estimation based on chloroplast sequence variation indicated that the Continental and Mediterranean clades might have been colonized independently during 0.65 ± 0.06 and 0.96 ± 0.1 million years ago (Mya) respectively. The findings of the study will enhance tall fescue breeding for persistence and productivity.
[Mh] Termos MeSH primário: Cloroplastos/genética
Festuca/genética
[Mh] Termos MeSH secundário: DNA de Plantas/genética
Festuca/metabolismo
Variação Genética/genética
Genômica/métodos
Genótipo
Repetições de Microssatélites/genética
Filogenia
Poaceae/genética
Análise de Sequência de DNA/métodos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180118
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191343


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[PMID]:28934356
[Au] Autor:de O Buanafina MM; Iyer PR; Buanafina MF; Shearer EA
[Ad] Endereço:Department of Biology, The Pennsylvania State University, University Park, PA, United States of America.
[Ti] Título:Reducing cell wall feruloylation by expression of a fungal ferulic acid esterase in Festuca arundinacea modifies plant growth, leaf morphology and the turnover of cell wall arabinoxylans.
[So] Source:PLoS One;12(9):e0185312, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A feature of cell wall arabinoxylan in grasses is the presence of ferulic acid which upon oxidative coupling by the action of peroxidases forms diferuloyl bridges between formerly separated arabinoxylans. Ferulate cross-linking is suspected of playing various roles in different plant processes. Here we investigate the role of cell wall feruloyaltion in two major processes, that of leaf growth and the turnover of cell wall arabinoxylans on leaf senescence in tall fescue using plants in which the level of cell wall ferulates has been reduced by targeted expression of the Aspergillus niger ferulic acid esterase A (FAEA) to the apoplast or Golgi. Analysis of FAE expressing plants showed that all the lines had shorter and narrower leaves compared to control, which may be a consequence of the overall growth rate being lower and occurring earlier in FAE expressing leaves than in controls. Furthermore, the final length of epidermal cells was shorter than controls, indicating that their expansion was curtailed earlier than in control leaves. This may be due to the observations that the deposition of both ether and ester linked monomeric hydroxycinnamic acids and ferulate dimerization stopped earlier in FAE expressing leaves but at a lower level than controls, and hydroxycinnamic acid deposition started to slow down when peroxidase levels increased. It would appear therefore that one of the possible mechanisms for controlling overall leaf morphology such as leaf length and width in grasses, where leaf morphology is highly variable between species, may be the timing of hydroxycinnamic acid deposition in the expanding cell walls as they emerge from cell division into the elongation zone, controlled partially by the onset of peroxidase activity in this region.
[Mh] Termos MeSH primário: Aspergillus niger/enzimologia
Hidrolases de Éster Carboxílico/genética
Parede Celular/metabolismo
Ácidos Cumáricos/metabolismo
Festuca/crescimento & desenvolvimento
Folhas de Planta/crescimento & desenvolvimento
Xilanos/metabolismo
[Mh] Termos MeSH secundário: Aspergillus niger/genética
Festuca/anatomia & histologia
Festuca/citologia
Festuca/genética
Expressão Gênica
Peroxidase/metabolismo
Fenótipo
Plantas Geneticamente Modificadas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coumaric Acids); 0 (Xylans); 9040-27-1 (arabinoxylan); AVM951ZWST (ferulic acid); EC 1.11.1.7 (Peroxidase); EC 3.1.1.- (Carboxylic Ester Hydrolases); EC 3.1.1.73 (feruloyl esterase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171018
[Lr] Data última revisão:
171018
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0185312


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[PMID]:28750041
[Au] Autor:Liu M; Hulting A; Mallory-Smith C
[Ad] Endereço:Department of Crop and Soil Science, Oregon State University, Corvallis, Oregon, United States of America.
[Ti] Título:Comparison of growth and physiological characteristics between roughstalk bluegrass and tall fescue in response to simulated waterlogging.
[So] Source:PLoS One;12(7):e0182035, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Roughstalk bluegrass (Poa trivialis) is a weed in cool season grass seed production fields in Oregon. Populations of this weed are often greater in fields prone to waterlogging. A greenhouse study was conducted to investigate the morphological and physiological differences between recently established roughstalk bluegrass and tall fescue (Lolium arundinaceum) plants in response to simulated waterlogging. Differences in root morphological development and root respiration were found between waterlogged tall fescue and roughstalk bluegrass. Plants after 4 weeks of waterlogging, leaf number, plant height, and root biomass were reduced more in tall fescue than in roughstalk bluegrass plants. The root length increased 6% in waterlogged tall fescue plants, and decreased 42% in waterlogged roughstalk bluegrass plants, which lead to a shallower root system in roughstalk bluegrass. Root aerenchyma area increased more in waterlogged roughstalk bluegrass than in tall fescue. Alcohol dehydrogenase and lactate dehydrogenase activities increased in the roots of both species, but not in the leaves. The increases were greater in tall fescue than in roughstalk bluegrass. Turf quality, aboveground biomass, photosynthetic capacity, and water-soluble carbohydrate concentrations were reduced by waterlogging, but there were no differences over time or species. Thus, the shallower root system, larger aerenchyma, and reduced fermentation rates were the characteristics most likely to contribute to better waterlogging tolerance in roughstalk bluegrass compared to tall fescue and invasion of roughstalk bluegrass in waterlogged cool season grass seed fields.
[Mh] Termos MeSH primário: Festuca/crescimento & desenvolvimento
Festuca/fisiologia
Poa/crescimento & desenvolvimento
Poa/fisiologia
Água/fisiologia
[Mh] Termos MeSH secundário: Biomassa
Carboidratos/análise
Clorofila/metabolismo
Festuca/metabolismo
Processamento de Imagem Assistida por Computador
L-Lactato Desidrogenase/metabolismo
Oxirredução
Fotossíntese
Folhas de Planta/anatomia & histologia
Folhas de Planta/citologia
Raízes de Plantas/anatomia & histologia
Raízes de Plantas/citologia
Poa/metabolismo
Solo/química
Solubilidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbohydrates); 0 (Soil); 059QF0KO0R (Water); 1406-65-1 (Chlorophyll); EC 1.1.1.27 (L-Lactate Dehydrogenase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182035


  4 / 473 MEDLINE  
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[PMID]:28688845
[Au] Autor:Sánchez V; López-Bellido FJ; Cañizares P; Rodríguez L
[Ad] Endereço:Department of Chemical Engineering, School of Civil Engineering, University of Castilla-La Mancha, Avenida Camilo José Cela, s/n, 13071, Ciudad Real, Spain.
[Ti] Título:Assessing the phytoremediation potential of crop and grass plants for atrazine-spiked soils.
[So] Source:Chemosphere;185:119-126, 2017 Oct.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pollution of soil and groundwater by atrazine has become an increasing environmental concern in the last decade. A phytoremediation test using plastic pots was conducted in order to assess the ability of several crops and grasses to remove atrazine from a soil of low permeability spiked with this herbicide. Four plant species were assessed for their ability to degrade or accumulate atrazine from soils: two grasses, i.e., ryegrass (Lolium perenne) and tall fescue (Festuca arundinacea), and two crops, i.e., barley (Hordeum vulgare) and maize (Zea mays). Three different doses of atrazine were used for the contamination of the pots: 2, 5 and 10 mg kg . 16 days after spiking, the initial amount of atrazine was reduced by 88.6-99.6% in planted pots, while a decrease of only 63.1-78.2% was found for the unplanted pots, thus showing the contribution of plants to soil decontamination. All the plant species were capable of accumulating atrazine and its N-dealkylated metabolites, i.e., deethylatrazine and deisopropylatrazine, in their tissues. Some toxic responses, such as biomass decreases and/or chlorosis, were observed in plants to a greater or lesser extent for initial soil doses of atrazine above 2 mg kg . Maize was the plant species with the highest ability to accumulate atrazine derivatives, reaching up to 38.4% of the initial atrazine added to the soil. Rhizosphere degradation/mineralization by microorganisms or plant enzymes, together with degradation inside the plants, have been proposed as the mechanisms that contributed to a higher extent than plant accumulation to explain the removal of atrazine from soils.
[Mh] Termos MeSH primário: Atrazina/análise
Biodegradação Ambiental
Poluentes do Solo/análise
[Mh] Termos MeSH secundário: Atrazina/análogos & derivados
Biomassa
Festuca/metabolismo
Herbicidas/análise
Lolium/metabolismo
Poaceae/metabolismo
Rizosfera
Solo
Zea mays/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Herbicides); 0 (Soil); 0 (Soil Pollutants); 0 (deethylatrazine); QJA9M5H4IM (Atrazine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170710
[St] Status:MEDLINE


  5 / 473 MEDLINE  
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[PMID]:28591168
[Au] Autor:Majka J; Ksiazczyk T; Kielbowicz-Matuk A; Kopecký D; Kosmala A
[Ad] Endereço:Institute of Plant Genetics, Polish Academy of Sciences, Poznan, Poland.
[Ti] Título:Exploiting repetitive sequences and BAC clones in Festuca pratensis karyotyping.
[So] Source:PLoS One;12(6):e0179043, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Festuca genus is thought to be the most numerous genus of the Poaceae family. One of the most agronomically important forage grasses, Festuca pratensis Huds. is treated as a model plant to study the molecular mechanisms associated with tolerance to winter stresses, including frost. However, the precise mapping of the genes governing stress tolerance in this species is difficult as its karyotype remains unrecognized. Only two F. pratensis chromosomes with 35S and 5S rDNA sequences can be easily identified, but its remaining chromosomes have not been distinguished to date. Here, two libraries derived from F. pratensis nuclear DNA with various contents of repetitive DNA sequences were used as sources of molecular probes for fluorescent in situ hybridisation (FISH), a BAC library and a library representing sequences most frequently present in the F. pratensis genome. Using FISH, six groups of DNA sequences were revealed in chromosomes on the basis of their signal position, including dispersed-like sequences, chromosome painting-like sequences, centromeric-like sequences, knob-like sequences, a group without hybridization signals, and single locus-like sequences. The last group was exploited to develop cytogenetic maps of diploid and tetraploid F. pratensis, which are presented here for the first time and provide a remarkable progress in karyotype characterization.
[Mh] Termos MeSH primário: Cromossomos de Plantas/genética
Festuca/genética
Sequências Repetitivas de Ácido Nucleico/genética
Estresse Fisiológico/genética
[Mh] Termos MeSH secundário: Temperatura Baixa
Diploide
Festuca/crescimento & desenvolvimento
Biblioteca Gênica
Hibridização Genética
Hibridização in Situ Fluorescente
Cariotipagem
RNA Ribossômico 5S/genética
Tetraploidia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 5S)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170913
[Lr] Data última revisão:
170913
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179043


  6 / 473 MEDLINE  
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[PMID]:28529468
[Au] Autor:Li Y; Hansson B; Ghatnekar L; Prentice HC
[Ad] Endereço:Department of Biology, Lund University, Lund, Sweden.
[Ti] Título:Contrasting patterns of nucleotide polymorphism suggest different selective regimes within different parts of the gene in L.
[So] Source:Hereditas;154:11, 2017.
[Is] ISSN:1601-5223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Phosphoglucose isomerase (PGI, EC 5.3.1.9) is an essential metabolic enzyme in all eukaryotes. An earlier study of the gene, which encodes cytosolic PGI in the grass L., revealed a marked difference in the levels of nucleotide polymorphism between the 5' and 3' portions of the gene. METHODS: In the present study, we characterized the sequence polymorphism in in more detail and examined possible explanations for the non-uniform pattern of nucleotide polymorphism across the gene. RESULTS: Our study confirms that the two portions of the gene show substantially different levels of DNA polymorphism and also suggests that the peptide encoded by the 3' portion of is functionally and structurally more important than that encoded by the 5' portion. Although there was some evidence of purifying selection ( / test) on the 5' portion of the gene, the signature of purifying selection was considerably stronger on the 3' portion of the gene ( / and McDonald-Kreitman tests). Several tests support the action of balancing selection within the 5' portion of the gene. Wall's and tests were significant only for the 5' portion of the gene. There were also marked peaks of nucleotide diversity, Tajima's and the / ratio at or around a codon site (within the 5' portion of the gene) that a previous study had suggested was subject to positive diversifying selection. CONCLUSIONS: Our results suggest that the two portions of the gene have been subject to different selective regimes. Purifying selection appears to have been the main force contributing to the relatively low level of polymorphism within the 3' portion of the sequence. In contrast, it is possible that balancing selection has contributed to the maintenance of the polymorphism within the 5' portion of the gene.
[Mh] Termos MeSH primário: Festuca/genética
Genes de Plantas
Glucose-6-Fosfato Isomerase/genética
Polimorfismo Genético
Seleção Genética
[Mh] Termos MeSH secundário: Sequência Conservada
DNA de Plantas/genética
Evolução Molecular
Festuca/enzimologia
Desequilíbrio de Ligação
Recombinação Genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); EC 5.3.1.9 (Glucose-6-Phosphate Isomerase)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170523
[St] Status:MEDLINE
[do] DOI:10.1186/s41065-017-0032-6


  7 / 473 MEDLINE  
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[PMID]:28494357
[Au] Autor:Wang B; Wang Q; Liu W; Liu X; Hou J; Teng Y; Luo Y; Christie P
[Ad] Endereço:Key Laboratory of Soil Environment and Pollution Remediation, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China; University of Chinese Academy of Sciences, Beijing 100049, China.
[Ti] Título:Biosurfactant-producing microorganism Pseudomonas sp. SB assists the phytoremediation of DDT-contaminated soil by two grass species.
[So] Source:Chemosphere;182:137-142, 2017 Sep.
[Is] ISSN:1879-1298
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytoremediation together with microorganisms may confer the advantages of both phytoremediation and microbial remediation of soils containing organic contaminants. In this system biosurfactants produced by Pseudomonas sp. SB may effectively help to increase the bioavailability of organic pollutants and thereby enhance their microbial degradation in soil. Plants may enhance the rhizosphere environment for microorganisms and thus promote the bioremediation of contaminants. In the present pot experiment study, dichlorodiphenyltrichloroethane (DDT) residues underwent an apparent decline after soil bioremediation compared with the original soil. The removal efficiency of fertilizer + tall fescue, fertilizer + tall fescue + Pseudomonas, fertilizer + perennial ryegrass, and fertilizer + perennial ryegrass + Pseudomonas treatments were 59.4, 65.6, 69.0, and 65.9%, respectively, and were generally higher than that in the fertilizer control (40.3%). Principal coordinates analysis (PCoA) verifies that plant species greatly affected the soil bacterial community irrespective of inoculation with Pseudomonas sp. SB. Furthermore, community composition analysis shows that Proteobacteria, Acidobacteria and Actinobacteria were the three dominant phyla in all groups. In particular, the relative abundance of Pseudomonas for fertilizer + tall fescue + Pseudomonas (0.25%) was significantly greater than fertilizer + tall fescue and this was related to the DDT removal efficiency.
[Mh] Termos MeSH primário: Biodegradação Ambiental
DDT/farmacocinética
Poaceae/metabolismo
Pseudomonas/metabolismo
[Mh] Termos MeSH secundário: Acidobacteria
DDT/isolamento & purificação
Fertilizantes
Festuca/metabolismo
Festuca/microbiologia
Lolium/metabolismo
Poaceae/microbiologia
Rizosfera
Microbiologia do Solo
Poluentes do Solo/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fertilizers); 0 (Soil Pollutants); CIW5S16655 (DDT)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170512
[St] Status:MEDLINE


  8 / 473 MEDLINE  
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[PMID]:28380578
[Au] Autor:Harlow BE; Goodman JP; Lynn BC; Flythe MD; Ji H; Aiken GE
[Ti] Título:Ruminal tryptophan-utilizing bacteria degrade ergovaline from tall fescue seed extract.
[So] Source:J Anim Sci;95(2):980-988, 2017 Feb.
[Is] ISSN:1525-3163
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The objectives of this study were to evaluate degradation of ergovaline in a tall fescue [ (Schreb.) Darbysh.] seed extract by rumen microbiota ex vivo and to identify specific bacteria capable of ergovaline degradation in vitro. Rumen cell suspensions were prepared by harvesting rumen fluid from fistulated wether goats ( = 3), straining, and differential centrifugation. Suspensions were dispensed into anaerobic tubes with added Trypticase with or without extract (∼10 µg kg ergovaline). Suspensions were incubated for 48 h at 39°C. Samples were collected at 0, 24, and 48 h for ergovaline analysis and enumeration of hyper-ammonia producing (HAB) and tryptophan-utilizing bacteria. Ergovaline values were analyzed by repeated measures using the mixed procedure of SAS. Enumeration data were log transformed for statistical analysis. When suspensions were incubated with extract, 11 to 15% of ergovaline disappearance was observed over 48 h ( = 0.02). After 24 h, suspensions with added extract had 10-fold less HAB than controls ( = 0.04), but treatments were similar by 48 h ( = 1.00). However, after 24 h and 48 h, suspensions with extract had 10-fold more tryptophan-utilizing bacteria ( < 0.01) that were later isolated and identified by their 16S RNA gene sequence as . The isolates and other known rumen pure cultures ( JB1, B159, HD4, B, F, MD1, SR) were evaluated for the ability to degrade ergovaline in vitro. Pure culture cell suspensions were incubated as described above and samples were taken at 0 and 48 h for ergovaline analysis. Data were analyzed using the ANOVA procedure of SAS. All HAB, including the isolates, tested degraded ergovaline (54 to 75%; < 0.05). B14 was also able to degrade ergovaline but to a lesser capacity (12%; < 0.05), but all other bacteria tested did not degrade ergovaline. The results of this study indicate which rumen bacteria may play an important role in ergovaline degradation and that microbiological strategies for controlling their activity could have ramifications for fescue toxicosis and other forms of ergotism in ruminants.
[Mh] Termos MeSH primário: Bactérias/metabolismo
Ergotaminas/metabolismo
Festuca/microbiologia
Extratos Vegetais/química
Sementes/química
Triptofano/metabolismo
[Mh] Termos MeSH secundário: Animais
Líquidos Corporais/microbiologia
Ergotaminas/química
Ergotismo
Cabras
Masculino
Rúmen/microbiologia
Sementes/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ergotamines); 0 (Plant Extracts); 059E2O9IV4 (ergovaline); 8DUH1N11BX (Tryptophan)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.2527/jas.2016.1128


  9 / 473 MEDLINE  
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[PMID]:28334706
[Au] Autor:Krivánková A; Kopecký D; Stoces S; Dolezel J; Hribová E
[Ad] Endereço:Institute of Experimental Botany, Centre of the Region Haná for Biotechnical and Agricultural Research, Olomouc, Czech Republic.
[Ti] Título:Repetitive DNA: A Versatile Tool for Karyotyping in Festuca pratensis Huds.
[So] Source:Cytogenet Genome Res;151(2):96-105, 2017.
[Is] ISSN:1424-859X
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:FISH is a useful method to identify individual chromosomes in a karyotype and to discover their structural changes accompanying genome evolution and speciation. DNA probes for FISH should be chromosome specific and/or exhibit specific patterns of distribution along each chromosome. Such probes are not available in many plants including meadow fescue (Festuca pratensis Huds.), an important forage grass species. In the present study, various DNA repeats identified in Illumina shotgun sequences specific to chromosome 4F of F. pratensis were used as probes for FISH to develop the molecular karyotype of meadow fescue and to reveal a long-range molecular organization of its chromosomes. Five tandem repeats produced specific patterns on individual chromosomes. Their use in combination with probes for rRNA genes enabled the establishment of the molecular karyotype of meadow fescue. Most of the mobile genetic elements were dispersed along all the chromosomes except for the DNA transposon CACTA, which was localized preferentially to telomeric and subtelomeric regions, and a putative LTR element, which was localized to (peri)centromeric regions. Cytogenetic mapping of the 5 tandem repeats in other accessions of meadow fescue showed a highly similar distribution and confirmed the versatility and robustness of these probes.
[Mh] Termos MeSH primário: Festuca/genética
Cariotipagem/métodos
Sequências de Repetição em Tandem
[Mh] Termos MeSH secundário: Cromossomos de Plantas
DNA de Plantas
Hibridização in Situ Fluorescente
Cariótipo
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170911
[Lr] Data última revisão:
170911
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170324
[St] Status:MEDLINE
[do] DOI:10.1159/000462915


  10 / 473 MEDLINE  
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[PMID]:28318794
[Au] Autor:Attinti R; Barrett KR; Datta R; Sarkar D
[Ad] Endereço:Department of Earth and Environmental Studies, Montclair State University, Montclair, NJ 07043, United States.
[Ti] Título:Ethylenediaminedisuccinic acid (EDDS) enhances phytoextraction of lead by vetiver grass from contaminated residential soils in a panel study in the field.
[So] Source:Environ Pollut;225:524-533, 2017 Jun.
[Is] ISSN:1873-6424
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Phytoextraction is a green remediation technology for cleaning contaminated soils. Application of chelating agents increases metal solubility and enhances phytoextraction. Following a successful greenhouse experiment, a panel study under field weather elucidated the efficiency of the chelating agent ethylenediaminedisuccinic acid (EDDS) on phytoextraction of lead (Pb) by vetiver grass, a hyperaccumulator of Pb, and a nonaccumulator fescue grass from residential soils contaminated with Pb-based paint from Baltimore, MD and San Antonio, TX. Three soils from each city with Pb content between 1000 and 2400 mg kg were chosen for the panel study. Sequential extraction revealed that Fe-Mn oxide (60-63%) and carbonate (25-33%) fractions of Pb dominated in Baltimore soils, whereas in San Antonio soils, Pb was primarily bound to the organic fraction (64-70%) because organic content was greater and, secondarily, to the Fe-Mn oxide (15-20%) fraction. Vetiver and fescue grasses were transplanted and grown on wood panels in the field with EDDS applied after 3 months and 13 months. Soil and leachate results indicated that EDDS applications increased Pb solubility in soils. Plant tissues results indicated enhanced the uptake of Pb by vetiver and showed that EDDS application promoted translocation of Pb from root to shoot. Average Pb concentration increased by 53% and 203% in shoots and by 73% and 84% in roots of vetiver after the first and second applications of EDDS, respectively. Concentrations in roots and shoots increased in all tested soils, regardless of soil pH or clay content. After the second application, average Pb concentrations in vetiver were higher than those in fescue by 3.6x in shoots and 8.3x in roots. Visual phytotoxic symptoms from increased bioavailable Pb from EDSS applications were observed in fescue but not in vetiver. This study demonstrated the potential of a chemically-catalyzed phytoremediation system as a cleanup method for lead-contaminated soils.
[Mh] Termos MeSH primário: Biodegradação Ambiental
Quelantes/metabolismo
Etilenodiaminas/metabolismo
Chumbo/metabolismo
Poluentes do Solo/metabolismo
Succinatos/metabolismo
[Mh] Termos MeSH secundário: Baltimore
Quelantes/química
Chrysopogon
Festuca
Chumbo/análise
Estudos Longitudinais
Raízes de Plantas/metabolismo
Poaceae/metabolismo
Solo/química
Poluentes do Solo/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 ((S,S)-ethylenediaminedisuccinate); 0 (Chelating Agents); 0 (Ethylenediamines); 0 (Soil); 0 (Soil Pollutants); 0 (Succinates); 2P299V784P (Lead)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170321
[St] Status:MEDLINE



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