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[PMID]:28464851
[Au] Autor:Millwood R; Nageswara-Rao M; Ye R; Terry-Emert E; Johnson CR; Hanson M; Burris JN; Kwit C; Stewart CN
[Ad] Endereço:Department of Plant Sciences, University of Tennessee, 252 Ellington Plant Sciences, 2431 Joe Johnson Dr., Knoxville, TN, 37996, USA.
[Ti] Título:Pollen-mediated gene flow from transgenic to non-transgenic switchgrass (Panicum virgatum L.) in the field.
[So] Source:BMC Biotechnol;17(1):40, 2017 05 02.
[Is] ISSN:1472-6750
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Switchgrass is C perennial grass species that is being developed as a cellulosic bioenergy feedstock. It is wind-pollinated and considered to be an obligate outcrosser. Genetic engineering has been used to alter cell walls for more facile bioprocessing and biofuel yield. Gene flow from transgenic cultivars would likely be of regulatory concern. In this study we investigated pollen-mediated gene flow from transgenic to nontransgenic switchgrass in a 3-year field experiment performed in Oliver Springs, Tennessee, U.S.A. using a modified Nelder wheel design. The planted area (0.6 ha) contained sexually compatible pollen source and pollen receptor switchgrass plants. One hundred clonal switchgrass 'Alamo' plants transgenic for an orange-fluorescent protein (OFP) and hygromycin resistance were used as the pollen source; whole plants, including pollen, were orange-fluorescent. To assess pollen movement, pollen traps were placed at 10 m intervals from the pollen-source plot in the four cardinal directions extending to 20 m, 30 m, 30 m, and 100 m to the north, south, west, and east, respectively. To assess pollination rates, nontransgenic 'Alamo 2' switchgrass clones were planted in pairs adjacent to pollen traps. RESULTS: In the eastward direction there was a 98% decrease in OFP pollen grains from 10 to 100 m from the pollen-source plot (Poisson regression, F1,8 = 288.38, P < 0.0001). At the end of the second and third year, 1,820 F seeds were collected from pollen recipient-plots of which 962 (52.9%) germinated and analyzed for their transgenic status. Transgenic progeny production detected in each pollen-recipient plot decreased with increased distance from the edge of the transgenic plot (Poisson regression, F1,15 = 12.98, P < 0.003). The frequency of transgenic progeny detected in the eastward plots (the direction of the prevailing wind) ranged from 79.2% at 10 m to 9.3% at 100 m. CONCLUSIONS: In these experiments we found transgenic pollen movement and hybridization rates to be inversely associated with distance. However, these data suggest pollen-mediated gene flow is likely to occur up to, at least, 100 m. This study gives baseline data useful to determine isolation distances and other management practices should transgenic switchgrass be grown commercially in relevant environments.
[Mh] Termos MeSH primário: Fluxo Gênico
Genes de Plantas
Panicum/genética
Pólen/genética
[Mh] Termos MeSH secundário: Proteínas Luminescentes/genética
Proteínas Luminescentes/metabolismo
Panicum/crescimento & desenvolvimento
Panicum/fisiologia
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/crescimento & desenvolvimento
Plantas Geneticamente Modificadas/fisiologia
Distribuição de Poisson
Sementes/crescimento & desenvolvimento
Sementes/fisiologia
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Luminescent Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170504
[St] Status:MEDLINE
[do] DOI:10.1186/s12896-017-0363-4


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[PMID]:28449656
[Au] Autor:Grant JN; Burris JN; Stewart CN; Lenaghan SC
[Ad] Endereço:Department of Plant Science, University of Tennessee, 2431 Joe Johnson Drive, Knoxville, TN, 37996, USA.
[Ti] Título:Improved tissue culture conditions for the emerging C model Panicum hallii.
[So] Source:BMC Biotechnol;17(1):39, 2017 04 27.
[Is] ISSN:1472-6750
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Panicum hallii Vasey (Hall's panicgrass) is a compact, perennial C grass in the family Poaceae, which has potential to enable bioenergy research for switchgrass (Panicum virgatum L.). Unlike P. hallii, switchgrass has a large genome, allopolyploidy, self-incompatibility, a long life cycle, and large stature-all suboptimal traits for rapid genetics research. Herein we improved tissue culture methodologies for two inbred P. hallii populations: FIL2 and HAL2, to enable further development of P. hallii as a model C plant. RESULTS: The optimal seed-derived callus induction medium was determined to be Murashige and Skoog (MS) medium supplemented with 40 mg L L-cysteine, 300 mg L L-proline, 3% sucrose, 1 g L casein hydrolysate, 3 mg L 2,4-dichlorophenoxyacetic acid (2,4-D), and 45 µg L 6-benzylaminopurine (BAP), which resulted in callus induction of 51 ± 29% for FIL2 and 81 ± 19% for HAL2. The optimal inflorescence-derived callus induction was observed on MP medium (MS medium supplemented with 2 g L L-proline, 3% maltose, 5 mg L 2,4-D, and 500 µg L BAP), resulting in callus induction of 100 ± 0.0% for FIL2 and 84 ± 2.4% for HAL2. Shoot regeneration rates of 11.5 ± 0.8 shoots/gram for FIL2 and 11.3 ± 0.6 shoots/gram for HAL2 were achieved using seed-induced callus, whereas shoot regeneration rates of 26.2 ± 2.6 shoots/gram for FIL2 and 29.3 ± 3.6 shoots/gram for HAL2 were achieved from inflorescence-induced callus. Further, cell suspension cultures of P. hallii were established from seed-derived callus, providing faster generation of callus tissue compared with culture using solidified media (1.41-fold increase for FIL2 and 3.00-fold increase for HAL2). CONCLUSIONS: Aside from abbreviated tissue culture times from callus induction to plant regeneration for HAL2, we noted no apparent differences between FIL2 and HAL2 populations in tissue culture performance. For both populations, the cell suspension cultures outperformed tissue cultures on solidified media. Using the methods developed in this work, P. hallii callus was induced from seeds immediately after harvest in a shorter time and with higher frequencies than switchgrass. For clonal propagation, P. hallii callus was established from R1 inflorescences, similar to switchgrass, which further strengthens the potential of this plant as a C model for genetic studies. The rapid cycling (seed-to-seed time) and ease of culture, further demonstrate the potential utility of P. hallii as a C model plant.
[Mh] Termos MeSH primário: Meios de Cultura/química
Panicum/crescimento & desenvolvimento
Técnicas de Cultura de Tecidos/métodos
[Mh] Termos MeSH secundário: Meios de Cultura/farmacologia
Germinação/efeitos dos fármacos
Inflorescência/crescimento & desenvolvimento
Modelos Biológicos
Células Vegetais/efeitos dos fármacos
Células Vegetais/fisiologia
Sementes/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171226
[Lr] Data última revisão:
171226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1186/s12896-017-0359-0


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[PMID]:28810219
[Au] Autor:Das L; Liu E; Saeed A; Williams DW; Hu H; Li C; Ray AE; Shi J
[Ad] Endereço:Biosystems and Agricultural Engineering Department, University of Kentucky, Lexington, KY 40546, United States.
[Ti] Título:Industrial hemp as a potential bioenergy crop in comparison with kenaf, switchgrass and biomass sorghum.
[So] Source:Bioresour Technol;244(Pt 1):641-649, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study takes combined field trial, lab experiment, and economic analysis approaches to evaluate the potential of industrial hemp in comparison with kenaf, switchgrass and biomass sorghum. Agronomy data suggest that the per hectare yield (5437kg) of industrial hemp stem alone was at a similar level with switchgrass and sorghum; while the hemp plants require reduced inputs. Field trial also showed that ∼1230kg/ha hemp grain can be harvested in addition to stems. Results show a predicted ethanol yield of ∼82gallons/dry ton hemp stems, which is comparable to the other three tested feedstocks. A comparative cost analysis indicates that industrial hemp could generate higher per hectare gross profit than the other crops if both hemp grains and biofuels from hemp stem were counted. These combined evaluation results demonstrate that industrial hemp has great potential to become a promising regional commodity crop for producing both biofuels and value-added products.
[Mh] Termos MeSH primário: Biocombustíveis
Cannabis
Panicum
Sorghum
[Mh] Termos MeSH secundário: Biomassa
Hibiscus
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170816
[St] Status:MEDLINE


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[PMID]:28803102
[Au] Autor:Oyedeji O; Daw CS; Labbe N; Ayers P; Abdoulmoumine N
[Ad] Endereço:Biosystems Engineering & Soil Science Department, University of Tennessee, Knoxville, TN 37996, United States.
[Ti] Título:Kinetics of the release of elemental precursors of syngas and syngas contaminants during devolatilization of switchgrass.
[So] Source:Bioresour Technol;244(Pt 1):525-533, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, the results from laboratory measurements of the devolatilization kinetics of switchgrass in a rapidly heated fixed bed reactor flushed with argon and operated at constant temperatures between 600 and 800°C was reported. Results indicate that switchgrass decomposes in two sequential stages during pyrolysis: stage I involves the evaporation and devolatilization of water and extractives and stage II involves that of hemicellulose, cellulose, and lignin. The estimated global activation energy for stage II increased from 52.80 to 59.39kJ/mol as the reactor temperature was increased from 600 to 800°C. The maximum conversion of carbon, hydrogen, oxygen, sulfur, and nitrogen ranged from 0.68 to 0.70, 0.90 to 0.95, 0.88 to 0.91, 0.70 to 0.80, and 0.55 to 0.66, respectively. The retention of alkali and alkaline earth metal (AAEM) species in the solid char after complete pyrolysis was significantly higher than in the original feed, indicating the importance of AAEM species in subsequent char processing.
[Mh] Termos MeSH primário: Panicum
[Mh] Termos MeSH secundário: Biomassa
Reatores Biológicos
Cinética
Lignina
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
9005-53-2 (Lignin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170814
[St] Status:MEDLINE


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[PMID]:28792541
[Au] Autor:Yimam YT; Ochsner TE; Fox GA
[Ad] Endereço:Department of Soil and Crop Sciences, Texas A&M University, College Station, Texas, United States of America.
[Ti] Título:Hydrologic cost-effectiveness ratio favors switchgrass production on marginal croplands over existing grasslands.
[So] Source:PLoS One;12(8):e0181924, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Switchgrass (Panicum virgatum L.) has attracted attention as a promising second generation biofuel feedstock. Both existing grasslands and marginal croplands have been suggested as targets for conversion to switchgrass, but the resulting production potentials and hydrologic impacts are not clear. The objectives of this study were to model switchgrass production on existing grasslands (scenario-I) and on marginal croplands that have severe to very severe limitations for crop production (scenario-II) and to evaluate the effects on evapotranspiration (ET) and streamflow. The Soil and Water Assessment Tool (SWAT) was applied to the 1063 km2 Skeleton Creek watershed in north-central Oklahoma, a watershed dominated by grasslands (35%) and winter wheat cropland (47%). The simulated average annual yield (2002-2011) for rainfed Alamo switchgrass for both scenarios was 12 Mg ha-1. Yield varied spatially under scenario-I from 6.1 to 15.3 Mg ha-1, while under scenario-II the range was from 8.2 to 13.8 Mg ha-1. Comparison of average annual ET and streamflow between the baseline simulation (existing land use) and scenario-I showed that scenario-I had 5.6% (37 mm) higher average annual ET and 27.7% lower streamflow, representing a 40.7 million m3 yr-1 streamflow reduction. Compared to the baseline, scenario-II had only 0.5% higher ET and 3.2% lower streamflow, but some monthly impacts were larger. In this watershed, the water yield reduction per ton of biomass production (i.e. hydrologic cost-effectiveness ratio) was more than 5X greater under scenario-I than under scenario-II. These results suggest that, from a hydrologic perspective, it may be preferable to convert marginal cropland to switchgrass production rather than converting existing grasslands.
[Mh] Termos MeSH primário: Agricultura/economia
Pradaria
Panicum
Rios
Água
[Mh] Termos MeSH secundário: Biocombustíveis/economia
Biomassa
Calibragem
Simulação por Computador
Conservação dos Recursos Naturais
Análise Custo-Benefício
Hidrologia
Oklahoma
Panicum/crescimento & desenvolvimento
Panicum/metabolismo
Transpiração Vegetal
Chuvas
Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 059QF0KO0R (Water)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170810
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0181924


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[PMID]:28577484
[Au] Autor:Zhao X; Liu J; Liu J; Yang F; Zhu W; Yuan X; Hu Y; Cui Z; Wang X
[Ad] Endereço:College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
[Ti] Título:Effect of ensiling and silage additives on biogas production and microbial community dynamics during anaerobic digestion of switchgrass.
[So] Source:Bioresour Technol;241:349-359, 2017 Oct.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Silage processing has a crucial positive impact on the methane yield of anaerobic treated substrates. Changes in the characteristics of switchgrass after ensiling with different additives and their effects on methane production and microbial community changes during anaerobic digestion were investigated. After ensiling (CK), methane yield was increased by 33.59% relative to that of fresh switchgrass (FS). In comparison with the CK treatment, methane production was improved by 17.41%, 13.08% and 8.72% in response to ensiling with LBr+X, LBr and X, respectively. A modified Gompertz model predicted that the optimum treatment was LBr+X, with a potential cumulative methane yield of 178.31mL/g total solids (TS) and a maximum biogas production rate of 44.39mL/g TS·d. Firmicutes and Bacteroidetes were the predominant bacteria in FS and silage switchgrass; however, the switchgrass treated with LBr+X was rich in Synergistetes, which was crucial for methane production.
[Mh] Termos MeSH primário: Biocombustíveis
Panicum
Silagem
[Mh] Termos MeSH secundário: Digestão
Metano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); OP0UW79H66 (Methane)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170604
[St] Status:MEDLINE


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[PMID]:28402308
[Au] Autor:El-Gendy AS; El-Kassas HI; Razek TM; Abdel-Latif H
[Ad] Endereço:Department of Construction Engineering, Faculty of Sciences and Engineering, The American University in Cairo, New Cairo, Egypt E-mail: ahmed.elgendy@aucegypt.edu.
[Ti] Título:Phyto-dewatering of sewage sludge using Panicum repens L.
[So] Source:Water Sci Technol;75(7-8):1667-1674, 2017 Apr.
[Is] ISSN:0273-1223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Experiments in the field environment have been conducted to study the growth of Panicum repens L., an aquatic plant, in the sewage sludge matrix. The experiments were also carried out to investigate the ability of this plant to dewater sewage sludge to increase the capacity of conventional drying beds. In addition, the ability of Panicum repens L. to reduce the sludge contents of certain elements (copper (Cu), Iron (Fe), Sodium (Na), lead (Pb), and Zinc (Zn)) was also investigated. All experiments were carried out in batch reactors. Different plant coverage densities were tested (0.00 to 27.3 kg/m ). The liquid sewage sludge was collected from a wastewater treatment plant in Helwan city, Cairo Governorate, Egypt. The collected sludge represents a mixture of the primary sludge and waste activated sludge before discharging into drying beds.
[Mh] Termos MeSH primário: Panicum/metabolismo
Esgotos/química
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Cobre/análise
Egito
Água/análise
Zinco/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sewage); 059QF0KO0R (Water); 789U1901C5 (Copper); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170919
[Lr] Data última revisão:
170919
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE
[do] DOI:10.2166/wst.2017.039


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[PMID]:28384528
[Au] Autor:Centeno DA; Solano XH; Castillo JJ
[Ad] Endereço:Grupo de Investigación en Bioquímica y Microbiología, GIBIM, Universidad Industrial de Santander, Bucaramanga, Colombia.
[Ti] Título:A new peroxidase from leaves of guinea grass (Panicum maximum): A potential biocatalyst to build amperometric biosensors.
[So] Source:Bioelectrochemistry;116:33-38, 2017 Aug.
[Is] ISSN:1878-562X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A new plant peroxidase was isolated from the leaves of guinea grass (Panicum maximum) and partially purified using a biphasic polymer system (poly(ethylene glycol) - ammonium sulfate) followed by size-exclusion chromatography and ultracentrifugation until obtaining a homogeneous extract containing a high peroxidase activity. The novel peroxidase was characterized as having a specific activity of 408U/mg and a molecular weight of 30kDa. The pH for its optimum activity was 8.0 and exhibited a high thermostability at 66°C with a k of 8.0×10 min . The best substrates for peroxidase from guinea grass are o-dianisidine and 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid). POD from guinea grass was directly immobilized on the surface of a graphene screen printed electrode and cyclic voltammograms in the presence of potassium ferrocyanide ([Fe(CN) ] ) as a redox species demonstrated an increase in the electron transfer process. The graphene- modified electrode exhibits excellent electrocatalytic activity to the reduction of H O , with a linear response in the 100µM to 3.5mM concentration range and a detection limit of 150µM. The new peroxidase from guinea grass allowed the modification of a graphene electrode providing a potential sensor detection system for determination of H O in real samples with some biomedical or environmental importance.
[Mh] Termos MeSH primário: Técnicas Biossensoriais/métodos
Panicum/enzimologia
Peroxidase/metabolismo
Folhas de Planta/enzimologia
[Mh] Termos MeSH secundário: Eletroquímica
Estabilidade Enzimática
Grafite/química
Peróxido de Hidrogênio/análise
Peróxido de Hidrogênio/química
Limite de Detecção
Peroxidase/química
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
7782-42-5 (Graphite); BBX060AN9V (Hydrogen Peroxide); EC 1.11.1.7 (Peroxidase)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170407
[St] Status:MEDLINE


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[PMID]:28279523
[Au] Autor:Bravetti MMDM; Vico RV; Carpinella MC; Ferrayoli CC; Palacios SM
[Ad] Endereço:Unidad Asociada Area Cs. Agr. Ing. Bio. y S.- CONICET - Universidad Católica de Córdoba, Avenida Armada Argentina 3555, CPA X5016DHK, Córdoba, Argentina.
[Ti] Título:Phytotoxic phenylpropanoids isolated from Ophryosporus charua (Griseb.) Hieron.
[So] Source:Phytochemistry;138:145-151, 2017 Jun.
[Is] ISSN:1873-3700
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bioguided isolation of the EtOH extract from the medicinal native plant, Ophryosporus charua, against Raphanus sativus, yielded three phenylpropanoids, charuol A [(Z)-4-((1S,2R)-3-acetoxy-1,2-dihydroxypropyl)phenyl) 2-methylbut-2-enoate], charuepoxide [(Z)-4-((2S,3R)-3-(acetoxymethyl oxiran-2-yl)phenyl) 2-methylbut-2-enoate] and charuol B [(Z)-4-((1R,2R)-3-acetoxy-1,2-dihydroxypropyl)phenyl) 2-methylbut-2-enoate]. Their structures and absolute configuration were established by extensive spectroscopic analyses. The effective concentrations for 50% inhibition of germination (ECg ) and root (ECr ) and shoot (ECs ) elongations were determined for these compounds against P. miliaceum (monocot) and Raphanus sativus (dicot). Charuol A was the most active in the inhibition of germination of P. miliaceum (ECg = 0.97 mM), followed by charuol B and charuepoxide, although charuol B was the most effective in regulating the root growth of P. miliaceum seedlings, with an ECr of 1.0 mM. Charuol A inhibited the germination of R. sativus, while its seedling development was also affected by all three compounds with different effectiveness. Charuol A was also highly effective in the 0.09-0.30 mM range against other test species such as Lactuca sativa, Eruca sativa, Allium ampeloprasum and Secale cereale.
[Mh] Termos MeSH primário: Asteraceae/química
Germinação/efeitos dos fármacos
Fenilpropionatos/química
Extratos Vegetais/química
Raphanus/efeitos dos fármacos
[Mh] Termos MeSH secundário: Estrutura Molecular
Panicum/efeitos dos fármacos
Componentes Aéreos da Planta/química
Plântulas/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenylpropionates); 0 (Plant Extracts)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170530
[Lr] Data última revisão:
170530
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE


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[PMID]:28264034
[Au] Autor:Wang H; Niu L; Fu C; Meng Y; Sang D; Yin P; Wu J; Tang Y; Lu T; Wang ZY; Tadege M; Lin H
[Ad] Endereço:Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, China.
[Ti] Título:Overexpression of the WOX gene STENOFOLIA improves biomass yield and sugar release in transgenic grasses and display altered cytokinin homeostasis.
[So] Source:PLoS Genet;13(3):e1006649, 2017 Mar.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lignocellulosic biomass can be a significant source of renewable clean energy with continued improvement in biomass yield and bioconversion strategies. In higher plants, the leaf blade is the central energy convertor where solar energy and CO2 are assimilated to make the building blocks for biomass production. Here we report that introducing the leaf blade development regulator STENOFOLIA (STF), a WOX family transcription factor, into the biofuel crop switchgrass, significantly improves both biomass yield and sugar release. We found that STF overexpressing switchgrass plants produced approximately 2-fold more dry biomass and release approximately 1.8-fold more solubilized sugars without pretreatment compared to controls. The biomass increase was attributed mainly to increased leaf width and stem thickness, which was also consistent in STF transgenic rice and Brachypodium, and appeared to be caused by enhanced cell proliferation. STF directly binds to multiple regions in the promoters of some cytokinin oxidase/dehydrogenase (CKX) genes and represses their expression in all three transgenic grasses. This repression was accompanied by a significant increase in active cytokinin content in transgenic rice leaves, suggesting that the increase in biomass productivity and sugar release could at least in part be associated with improved cytokinin levels caused by repression of cytokinin degrading enzymes. Our study provides a new tool for improving biomass feedstock yield in bioenergy crops, and uncovers a novel mechanistic insight in the function of STF, which may also apply to other repressive WOX genes that are master regulators of several key plant developmental programs.
[Mh] Termos MeSH primário: Citocininas/genética
Medicago truncatula/genética
Panicum/genética
Proteínas de Plantas/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Biocombustíveis
Biomassa
Brachypodium/genética
Brachypodium/metabolismo
Metabolismo dos Carboidratos
Proliferação Celular
Imunoprecipitação da Cromatina
Produtos Agrícolas/genética
Produtos Agrícolas/metabolismo
Citocininas/metabolismo
DNA de Plantas/genética
Regulação da Expressão Gênica de Plantas
Proteínas de Fluorescência Verde/metabolismo
Homeostase
Análise de Sequência com Séries de Oligonucleotídeos
Oryza/genética
Oryza/metabolismo
Oxirredutases/genética
Panicum/metabolismo
Fenótipo
Folhas de Planta/metabolismo
Proteínas de Plantas/metabolismo
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/metabolismo
Fatores de Transcrição/metabolismo
Transgenes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 0 (Cytokinins); 0 (DNA, Plant); 0 (Plant Proteins); 0 (Transcription Factors); 147336-22-9 (Green Fluorescent Proteins); EC 1.- (Oxidoreductases); EC 1.5.99.12 (cytokinin oxidase)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170606
[Lr] Data última revisão:
170606
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170307
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006649



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