Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.822.793 [Categoria DeCS]
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[PMID]:28750041
[Au] Autor:Liu M; Hulting A; Mallory-Smith C
[Ad] Endereço:Department of Crop and Soil Science, Oregon State University, Corvallis, Oregon, United States of America.
[Ti] Título:Comparison of growth and physiological characteristics between roughstalk bluegrass and tall fescue in response to simulated waterlogging.
[So] Source:PLoS One;12(7):e0182035, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Roughstalk bluegrass (Poa trivialis) is a weed in cool season grass seed production fields in Oregon. Populations of this weed are often greater in fields prone to waterlogging. A greenhouse study was conducted to investigate the morphological and physiological differences between recently established roughstalk bluegrass and tall fescue (Lolium arundinaceum) plants in response to simulated waterlogging. Differences in root morphological development and root respiration were found between waterlogged tall fescue and roughstalk bluegrass. Plants after 4 weeks of waterlogging, leaf number, plant height, and root biomass were reduced more in tall fescue than in roughstalk bluegrass plants. The root length increased 6% in waterlogged tall fescue plants, and decreased 42% in waterlogged roughstalk bluegrass plants, which lead to a shallower root system in roughstalk bluegrass. Root aerenchyma area increased more in waterlogged roughstalk bluegrass than in tall fescue. Alcohol dehydrogenase and lactate dehydrogenase activities increased in the roots of both species, but not in the leaves. The increases were greater in tall fescue than in roughstalk bluegrass. Turf quality, aboveground biomass, photosynthetic capacity, and water-soluble carbohydrate concentrations were reduced by waterlogging, but there were no differences over time or species. Thus, the shallower root system, larger aerenchyma, and reduced fermentation rates were the characteristics most likely to contribute to better waterlogging tolerance in roughstalk bluegrass compared to tall fescue and invasion of roughstalk bluegrass in waterlogged cool season grass seed fields.
[Mh] Termos MeSH primário: Festuca/crescimento & desenvolvimento
Festuca/fisiologia
Poa/crescimento & desenvolvimento
Poa/fisiologia
Água/fisiologia
[Mh] Termos MeSH secundário: Biomassa
Carboidratos/análise
Clorofila/metabolismo
Festuca/metabolismo
Processamento de Imagem Assistida por Computador
L-Lactato Desidrogenase/metabolismo
Oxirredução
Fotossíntese
Folhas de Planta/anatomia & histologia
Folhas de Planta/citologia
Raízes de Plantas/anatomia & histologia
Raízes de Plantas/citologia
Poa/metabolismo
Solo/química
Solubilidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Carbohydrates); 0 (Soil); 059QF0KO0R (Water); 1406-65-1 (Chlorophyll); EC 1.1.1.27 (L-Lactate Dehydrogenase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182035


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[PMID]:28378974
[Au] Autor:Stuper-Szablewska K; Perkowski J
[Ad] Endereço:Department of Chemistry, University of Life Sciences, Poznan, Poland.
[Ti] Título:Level of contamination with mycobiota and contents of mycotoxins from the group of trichothecenes in grain of wheat , oats, barley, rye and triticale harvested in Poland in 2006- 2008.
[So] Source:Ann Agric Environ Med;24(1):49-55, 2017 Mar 01.
[Is] ISSN:1898-2263
[Cp] País de publicação:Poland
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION AND OBJECTIVE: The risk of cereal exposure to microbial contamination is high and possible at any time, starting from the period of plant vegetation, through harvest, up to the processing, storage and transport of the final product. Contents of mycotoxins in grain are inseparably connected with the presence of fungal biomass, the presence of which may indicate the occurrence of a fungus, and indirectly also products of its metabolism. MATERIALS AND METHOD: Analyses were conducted on 378 grain samples of wheat, triticale, barley, rye and oats collected from grain silos located at grain purchase stations and at mills in Poland in 2006, 2007 and 2008. The concentrations of ERG and mycotoxins from the group of trichothecenes, as well as CFU numbers were analysed. RESULTS: The tested cereals were characterised by similarly low concentrations of both the investigated fungal metabolites and the level of microscopic fungi. However, conducted statistical analyses showed significant variation between tested treatments. Oat and rye grain contained the highest amounts of ERG, total toxins and CFU. In turn, the lowest values of investigated parameters were found in grain of wheat and triticale. CONCLUSIONS: Chemometric analyses, based on the results of chemical and microbiological tests, showed slight differences between contents of analysed metabolites between the years of the study, and do not confirm the observations on the significance of the effect of weather conditions on the development of mycobiota and production of mycotoxins; however, it does pertain to treatments showing no significant infestation. Highly significant correlations between contents of trichothecenes and ERG concentration (higher than in the case of the correlation of the total toxin concentrations/log cfu/g), indicate that the level of this metabolite is inseparably connected with mycotoxin contents in grain.
[Mh] Termos MeSH primário: Microbiologia de Alimentos
Fungos/isolamento & purificação
Micotoxinas/análise
Poa/microbiologia
Tricotecenos/análise
[Mh] Termos MeSH secundário: Contagem de Colônia Microbiana
Grãos Comestíveis/química
Grãos Comestíveis/microbiologia
Ergosterol/análise
Poa/química
Polônia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Mycotoxins); 0 (Trichothecenes); Z30RAY509F (Ergosterol)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.5604/12321966.1230733


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[PMID]:28369130
[Au] Autor:Shaw AN; Mummey DL
[Ad] Endereço:MPG Ranch, Missoula, Montana, United States of America.
[Ti] Título:Poa secunda local collections and commercial releases: A genotypic evaluation.
[So] Source:PLoS One;12(4):e0173221, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The genetics of native plants influence the success of ecological restoration, yet genetic variability of local seed collections and commercial seed releases remains unclear for most taxa. Poa secunda, a common native grass species in Intermountain West grasslands and a frequent component of restoration seed mixes, is one such species. Here, we evaluate the genetic variation of local Poa secunda collections in the context of wild populations and commercial seed releases. We evaluated AFLP markers for seven Poa secunda collections made over a 4000-hectare area and four commercial releases (High Plains, MT-1, Opportunity, and Sherman). We compare the genetic distance and distribution of genetic variation within and between local collections and commercial releases. The extent and patterns of genetic variation in our local collections indicate subtle site differences with most variation occurring within rather than between collections. Identical genetic matches were usually, but not always, found within 5 m2 collection sites. Our results suggest that the genetic variation in two Poa secunda releases (High Plains and MT-1) is similar to our local collections. Our results affirm that guidelines for Poa secunda seed collection should follow recommendations for selfing species, by collecting from many sites over large individual sites.
[Mh] Termos MeSH primário: Poa/genética
[Mh] Termos MeSH secundário: Adaptação Fisiológica/genética
Agricultura
Análise do Polimorfismo de Comprimento de Fragmentos Amplificados
Apomixia
Conservação dos Recursos Naturais
DNA de Plantas/genética
DNA de Plantas/isolamento & purificação
Ecossistema
Variação Genética
Genótipo
Montana
Poa/fisiologia
Sementes/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0173221


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[PMID]:27803002
[Au] Autor:Kong RS; Henry HA
[Ad] Endereço:Department of Biology, University of Western Ontario, 1151 Richmond St. N., London, ON, Canada, N6A 5B7 rkong9@uwo.ca.
[Ti] Título:Prior exposure to freezing stress enhances the survival and recovery of Poa pratensis exposed to severe drought.
[So] Source:Am J Bot;103(11):1890-1896, 2016 Nov.
[Is] ISSN:1537-2197
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PREMISE OF THE STUDY: Both freezing and drought cause cellular dehydration, and they elicit similar increases in protective compounds, which suggests that these stresses could potentially interact. We examined whether the physiological changes that occur in response to freezing in the fall and spring could affect subsequent survival and growth after summer drought. METHODS: We froze Poa pratensis tillers in the late fall, early spring, or late spring at 0, -5, or -10°C for 3 d and then subjected them to no drought (-0.025 MPa), moderate drought (-0.140 MPa), or severe drought (-0.250 MPa) for 3 wk in the summer. We quantified survival and total biomass after a 3-wk recovery period, and we determined leaf soluble sugar concentrations before and then 0, 30, and 55 d after freezing. KEY RESULTS: For survival and biomass, there were significant interactions between freezing and drought. Spring frozen tillers had the highest biomass and survival following severe drought, whereas fall freezing did not significantly increase the biomass of tillers following the severe drought. Increased drought tolerance after spring freezing did not appear to be associated with increased soluble sugar content, given that the freezing effects on leaf glucose, fructose, and sucrose content were absent 55 d post freezing. CONCLUSIONS: Our results demonstrate that multiple stresses that occur over different seasons can interact; this interaction is highly relevant to herbaceous species in northern temperate regions that are experiencing more intense and frequent stress as a result of changes in snow cover and extreme climatic events.
[Mh] Termos MeSH primário: Aclimatação
Poa/fisiologia
[Mh] Termos MeSH secundário: Biomassa
Desidratação
Secas
Congelamento
Folhas de Planta/crescimento & desenvolvimento
Folhas de Planta/fisiologia
Poa/crescimento & desenvolvimento
Estações do Ano
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170515
[Lr] Data última revisão:
170515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161103
[St] Status:MEDLINE


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[PMID]:27373539
[Au] Autor:Giussani LM; Gillespie LJ; Scataglini MA; Negritto MA; Anton AM; Soreng RJ
[Ad] Endereço:Instituto de Botánica Darwinion, San Isidro, Buenos Aires, Argentina lgiussani@darwin.edu.ar.
[Ti] Título:Breeding system diversification and evolution in American Poa supersect. Homalopoa (Poaceae: Poeae: Poinae).
[So] Source:Ann Bot;118(2):281-303, 2016 Aug.
[Is] ISSN:1095-8290
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND AIMS: Poa subgenus Poa supersect. Homalopoa has diversified extensively in the Americas. Over half of the species in the supersection are diclinous; most of these are from the New World, while a few are from South-East Asia. Diclinism in Homalopoa can be divided into three main types: gynomonoecism, gynodioecism and dioecism. Here the sampling of species of New World Homalopoa is expanded to date its origin and diversification in North and South America and examine the evolution and origin of the breeding system diversity. METHODS: A total of 124 specimens were included in the matrix, of which 89 are species of Poa supersect. Homalopoa sections Acutifoliae, Anthochloa, Brizoides, Dasypoa, Dioicopoa, Dissanthelium, Homalopoa sensu lato (s.l.), Madropoa and Tovarochloa, and the informal Punapoa group. Bayesian and parsimony analyses were conducted on the data sets based on four markers: the nuclear ribosomal internal tanscribed spacer (ITS) and external transcribed spacer (ETS), and plastid trnT-L and trnL-F. Dating analyses were performed on a reduced Poa matrix and enlarged Poaceae outgroup to utilize fossils as calibration points. A relaxed Bayesian molecular clock method was used. KEY RESULTS: Hermaphroditism appears to be pleisiomorphic in the monophyletic Poa supersect. Homalopoa, which is suggested to have originated in Eurasia 8·4-4·2 million years ago (Mya). The ancestor of Poa supersect. Homalopoa radiated throughout the New World in the Late Miocene-Early Pliocene, with major lineages originating during the Pliocene to Pleistocene (5-2 Mya). Breeding systems are linked to geographic areas, showing an evolutionary pattern associated with different habitats. At least three major pathways from hermaphroditism to diclinism are inferred in New World Homalopoa: two leading to dioecism, one via gynodioecism in South America and another directly from hermaphroditism in North America, a result that needs to be checked with a broader sampling of diclinous species in North America. A third pathway leads from hermaphroditism to gynomonoecism in Andean species of South America, with strictly pistillate species evolving in the highest altitudes. CONCLUSIONS: Divergence dating provides a temporal context to the evolution of breeding systems in New World Poa supersect. Homalopoa The results are consistent with the infrageneric classification in part; monophyletic sections are confirmed, it is proposed to reclassify species of sect. Acutifoliae, Dasypoa and Homalopoa s.l. and it is acknowledged that revision of the infrageneric taxonomy of the gynomonoecious species is needed.
[Mh] Termos MeSH primário: Melhoramento Vegetal
Poa/genética
Poaceae/genética
[Mh] Termos MeSH secundário: Teorema de Bayes
Evolução Biológica
DNA de Plantas/química
DNA de Plantas/genética
DNA Espaçador Ribossômico/química
DNA Espaçador Ribossômico/genética
Flores/classificação
Flores/genética
Fósseis
América do Norte
Filogenia
Plastídeos/genética
Poa/classificação
Poaceae/classificação
Análise de Sequência de DNA
América do Sul
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); 0 (DNA, Ribosomal Spacer)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170727
[Lr] Data última revisão:
170727
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160705
[St] Status:MEDLINE
[do] DOI:10.1093/aob/mcw108


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[PMID]:27353253
[Au] Autor:Kostromytska OS; Koppenhöfer AM
[Ad] Endereço:Department of Entomology,Rutgers University,New Brunswick,NJ 08901,USA.
[Ti] Título:Responses of Poa annua and three bentgrass species (Agrostis spp.) to adult and larval feeding of annual bluegrass weevil, Listronotus maculicollis (Coleoptera: Curculionidae).
[So] Source:Bull Entomol Res;106(6):729-739, 2016 Dec.
[Is] ISSN:1475-2670
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The annual bluegrass weevil (ABW), Listronotus maculicollis Kirby, is an economically important pest of short-cut turfgrass in Eastern North America. Wide spread insecticide resistance warrants the development of alternative management strategies for this pest. ABW damage typically occurs in areas with a high percentage of annual bluegrass, Poa annua L., the preferred ABW host. Damage to bentgrasses, Agrostis spp., is much rarer and usually less severe. To aid the implementation of host plant resistance as an alternative ABW management strategy we investigated the tolerance of three bentgrass species to ABW feeding. Responses of P. annua, creeping bentgrass, Agrostis stolonifera L., colonial bentgrass, Agrostis capillaris L., and velvet bentgrass, Agrostis canina L., to adult and larval feeding were compared in greenhouse experiments. Grass responses were measured as visual damage, dry weight of the grass stems and leaves, color, density and overall grass quality. To determine possible mechanisms of grass tolerance constitutive fiber and silicon content were also determined. The three bentgrass species tolerated 2-3 times higher numbers of ABW adults and larvae than P. annua before displaying any significant quality decrease. Creeping bentgrass had the lowest damage ratings. ABW infestation caused higher plant yield reduction in P. annua (up to 42%) than in bentgrasses. Observed differences among the grass species in fiber and silicon content in the plant tissue are unlikely to play a role in the resistance of bentgrasses to ABW. Our findings clearly show that A. stolonifera is the best grass species for the implementation of host plant resistance in ABW management.
[Mh] Termos MeSH primário: Agrostis/fisiologia
Herbivoria
Poa/fisiologia
Gorgulhos/fisiologia
[Mh] Termos MeSH secundário: Animais
Celulose/metabolismo
Lignina/metabolismo
Polissacarídeos/metabolismo
Densidade Demográfica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Polysaccharides); 8024-50-8 (hemicellulose); 9004-34-6 (Cellulose); 9005-53-2 (Lignin)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160630
[St] Status:MEDLINE


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[PMID]:27262339
[Au] Autor:Chen X; Fukushi K
[Ad] Endereço:Department of Urban Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan. Electronic address: chenxiaochen1984@gmail.com.
[Ti] Título:Development of an innovative decentralized treatment system for the reclamation and reuse of strong wastewater from rural community: Effects of elevated CO2 concentrations.
[So] Source:J Environ Manage;180:401-8, 2016 Sep 15.
[Is] ISSN:1095-8630
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In a previous study, a soil-plant-based natural treatment system was successfully developed for post-treatment of anaerobically digested strong wastewater full of potential nutrients (nitrogen, phosphorus, and potassium). For upgraded performance, an innovative decentralized treatment system was further developed, in which an anaerobic digestion stage and a natural treatment system stage are placed within a greenhouse. This allows the CO2 generated by the processing of wastewater and biogas consumption to be sequestrated within the greenhouse for elevating its concentration level and potentially enhance nutrient removal and recovery from the applied wastewater. To investigate the feasibility of the system, a bench-scale experiment was conducted using CO2 chambers. Valuable Kentucky bluegrass was planted in two soil types (red ball earth and black soil) at three CO2 concentrations (340 ppm, 900 ppm, and 1400 ppm). The results confirmed the positive effects of elevated CO2 concentration on the biomass production and turf quality of Kentucky bluegrass as well as the resulting higher nutrient recovery efficiencies. More importantly, it was demonstrated that the elevated CO2 concentration significantly stimulated the soil nitrifying microorganisms and thus improved the nitrogen removal efficiency (a critical issue in ecological wastewater treatment). A CO2 concentration of 1400 ppm is therefore recommended for use in the system. The mechanism underlying this phenomenon was shown to be an indirect effect, in which the higher CO2 concentration first positively influenced growing plants, which then stimulated the soil nitrifier communities. The effects of soil type (a design parameter) and hydraulic and nutrient loading rates (an operational parameter) on system performance were also examined. The results favored black soil for system establishment. Based on the findings of this study, our proposed system is thought to have the potential to be scaled up and adopted by rural communities worldwide for the reclamation and reuse of strong wastewater, addressing the agricultural non-point source pollution, and achieving the sustainable development.
[Mh] Termos MeSH primário: Dióxido de Carbono/metabolismo
Reciclagem/métodos
Eliminação de Resíduos Líquidos/métodos
[Mh] Termos MeSH secundário: Agricultura
Biomassa
Conservação dos Recursos Naturais
Poa/crescimento & desenvolvimento
Poa/metabolismo
Microbiologia do Solo
Águas Residuais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Waste Water); 142M471B3J (Carbon Dioxide)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160606
[St] Status:MEDLINE


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[PMID]:27236444
[Au] Autor:Saud S; Yajun C; Fahad S; Hussain S; Na L; Xin L; Alhussien SA
[Ad] Endereço:College of Horticulture, Northeast Agricultural University Harbin, Heilongjiang, China, 150030.
[Ti] Título:Silicate application increases the photosynthesis and its associated metabolic activities in Kentucky bluegrass under drought stress and post-drought recovery.
[So] Source:Environ Sci Pollut Res Int;23(17):17647-55, 2016 Sep.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Drought stress is the most pervasive threat to plant growth, which disrupts the photosynthesis and its associated metabolic activities, while silicate (Si) application may have the potential to alleviate the damaging effects of drought on plant growth. In present study, the role of Si in regulating the photosynthesis and its associated metabolic events in Kentucky bluegrass (cv. Arcadia) were investigated under drought stress. Drought stress and four levels (0, 200, 400, 800 mg L(-1)) of Si (Na2SiO3.9H2O) were imposed on 1-year-old plants removed from field and cultured under glasshouse conditions. After 20 days of drought stress, the plants were re-watered to reach soil field capacity for the examination of recovery on the second and the seventh day. The experiment was arranged in completely randomized design replicated four times. Drought stress severely decreased the photosynthesis, water use efficiency, stomatal conductance, cholorophyll contents, Rubisco activity, and Rubisco activation state in Kentucky bluegrass. Nevertheless, application of Si had a positive influence on all these attributes, particularly under stress conditions. As compared to control, Si application at 400 mg L(-1) recorded 78, 64, and 48 % increase in photosynthesis, Rubisco initial activity, and Rubisco total activity, respectively, at 20 days of drought. Higher photosynthesis and higher Rubisco activity in Si-applied treatments suggest that Si may have possible (direct or indirect) role in maintenance of more active Rubisco enzyme and Rubisco activase and more stable proteins for carbon assimilation under stress conditions, which needs to be elucidated in further studies.
[Mh] Termos MeSH primário: Secas
Fotossíntese/efeitos dos fármacos
Poa/efeitos dos fármacos
Poa/metabolismo
Silicatos/farmacologia
Estresse Fisiológico/efeitos dos fármacos
[Mh] Termos MeSH secundário: Ribulose-Bifosfato Carboxilase/metabolismo
Solo
Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Silicates); 0 (Soil); 059QF0KO0R (Water); EC 4.1.1.39 (Ribulose-Bisphosphate Carboxylase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160530
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-016-6957-x


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[PMID]:27129471
[Au] Autor:Ni Y; Guo N; Zhao Q; Guo Y
[Ad] Endereço:College of Agronomy and Biotechnology, Southwest University, Chongqing, 400716, China.
[Ti] Título:Identification of candidate genes involved in wax deposition in Poa pratensis by RNA-seq.
[So] Source:BMC Genomics;17:314, 2016 Apr 29.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The cuticular wax plays important roles in plant resistance to various biotic and abiotic stresses. Understanding the synthesis and secretion of cuticular waxes is necessary in utilizing cuticular waxes to improve crop productivity and plant ecological adaptation. Due to the lack of genomic resources, little genetic research on cuticular wax deposition has been focused on Poa pratensis, a perennial forage and turf grass species that is widely distributed under various habitats. In this study, we performed de novo transcriptome sequencing to explore differentially expressed genes between the leaf non-elongation zone (NEZm) and the emerged blade zone (EBZ) and to identify genes related to cuticular wax deposition. RESULTS: A total of 77,707,414 high quality reads were obtained from llumina HiSeq 2500 platform, which were then assembled into 106,766 unigenes. Among them, 6019 unigenes showed significant differences in expression between NEZm and EBZ. In our assembled sequences, 3087 SSRs molecular markers were discovered. All the unigenes were searched against the NR, Swissprot, GO, COG, and KEGG databases using BLAST program for functional annotation. From 3156 unigenes with more expression in NEZm compared to EBZ, a number of unigenes involved in very long chain fatty acids (VLCFAs) and cuticular wax biosynthesis, transportation and regulation were identified. Several unigenes related to defense response and epidermal patterning were also found. Twelve putative genes involved in VLCFAs and cuticular wax biosynthesis were further analyzed for their expressions using qRT-PCR. CONCLUSIONS: The transcriptome of P. pratensis leaf was deep sequenced, de novo assembled and annotated, and the candidate genes potentially involved in VLCFAs and cuticular wax biosynthesis, secretion and regulation in P. pratensis were identified. This provides fundamental genetic resources in improving plant adaptation to abiotic and biotic stresses.
[Mh] Termos MeSH primário: Genes de Plantas
Poa/genética
Transcriptoma
Ceras/química
[Mh] Termos MeSH secundário: Regulação da Expressão Gênica de Plantas
Sequenciamento de Nucleotídeos em Larga Escala
Repetições de Microssatélites
Anotação de Sequência Molecular
Folhas de Planta/química
RNA de Plantas/genética
Análise de Sequência de RNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Plant); 0 (Waxes)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160501
[St] Status:MEDLINE
[do] DOI:10.1186/s12864-016-2641-2


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[PMID]:27010560
[Au] Autor:Gan L; Di R; Chao Y; Han L; Chen X; Wu C; Yin S
[Ad] Endereço:Institute of Turfgrass Science, Beijing Forestry University, Beijing, 100083, China.
[Ti] Título:De Novo Transcriptome Analysis for Kentucky Bluegrass Dwarf Mutants Induced by Space Mutation.
[So] Source:PLoS One;11(3):e0151768, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Kentucky bluegrass (Poa pratensis L.) is a major cool-season turfgrass requiring frequent mowing. Utilization of cultivars with slow growth is a promising method to decrease mowing frequency. In this study, two dwarf mutant selections of Kentucky bluegrass (A12 and A16) induced by space mutation were analyzed for the differentially expressed genes compared with the wild type (WT) by the high-throughput RNA-Seq technology. 253,909 unigenes were obtained by de novo assembly. 24.20% of the unigenes had a significant level of amino acid sequence identity to Brachypodium distachyon proteins, followed by Hordeum vulgare with 18.72% among the non-redundant (NR) Blastx top hits. Assembled unigenes were associated with 32 pathways using KEGG orthology terms and their respective KEGG maps. Between WT and A16 libraries, 4,203 differentially expressed genes (DEGs) were identified, whereas there were 883 DEGs between WT and A12 libraries. Further investigation revealed that the DEG pathways were mainly involved in terpenoid biosynthesis and plant hormone metabolism, which might account for the differences of plant height and leaf blade color between dwarf mutant and WT plants. Our study presents the first comprehensive transcriptomic data and gene function analysis of Poa pratensis L., providing a valuable resource for future studies in plant dwarfing breeding and comparative genome analysis for Pooideae plants.
[Mh] Termos MeSH primário: Meio Ambiente Extraterreno
Mutação
Poa/genética
Sementes/genética
[Mh] Termos MeSH secundário: Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Ontologia Genética
Genes de Plantas
Kentucky
Mutagênese
Poa/crescimento & desenvolvimento
Sementes/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160325
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0151768



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