Base de dados : MEDLINE Pesquisa : B01.650.940.800.575.912.250.822.835 [Categoria DeCS] Referências encontradas : 2132 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 28987406 [Au] Autor: Szewczyk R; Kusmierska A; Bernat P [Ad] Endereço: Department of Industrial Microbiology and Biotechnology, Institute of Microbiology, Biotechnology and Immunology, Faculty of Biology and Environmental Protection, University of Lódz, Banacha 12/16, 90-237 Lódz, Poland. Electronic address: rafal.szewczyk@biol.uni.lodz.pl. [Ti] Título: Ametryn removal by Metarhizium brunneum: Biodegradation pathway proposal and metabolic background revealed. [So] Source: Chemosphere;190:174-183, 2018 Jan. [Is] ISSN: 1879-1298 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Ametryn is a representative of a class of s-triazine herbicides absorbed by plant roots and leaves and characterized as a photosynthesis inhibitor. It is still in use in some countries in the farming of pineapples, soybean, corn, cotton, sugar cane or bananas; however, due to the adverse effects of s-triazine herbicides on living organisms use of these pesticides in the European Union has been banned. In the current study, we characterized the biodegradation of ametryn (100 mg L ) by entomopathogenic fungal cosmopolite Metarhizium brunneum. Ametryn significantly inhibited the growth and glucose uptake in fungal cultures. The concentration of the xenobiotic drops to 87.75 mg L at the end of culturing and the biodegradation process leads to formation of four metabolites: 2-hydroxy atrazine, ethyl hydroxylated ametryn, S-demethylated ametryn and deethylametryn. Inhibited growth is reflected in the metabolomics data, where significant differences in concentrations of L-proline, gamma-aminobutyric acid, L-glutamine, 4-hydroxyproline, L-glutamic acid, ornithine and L-arginine were observed in the presence of the xenobiotic when compared to control cultures. The metabolomics data demonstrated that the presence of ametryn in the fungal culture induced oxidative stress and serious disruptions of the carbon and nitrogen metabolism. Our results provide deeper insights into the microorganism strategy for xenobiotic biodegradation which may result in future enhancements to ametryn removal by the tested strain. [Mh] Termos MeSH primário: Herbicidas/isolamento & purificação Metarhizium/metabolismo Triazinas/metabolismo [Mh] Termos MeSH secundário: Atrazina Biodegradação Ambiental Carbono/metabolismo Ácido Glutâmico Herbicidas/metabolismo Herbicidas/farmacologia Nitrogênio/metabolismo Estresse Oxidativo/efeitos dos fármacos Prolina Saccharum/metabolismo Triazinas/isolamento & purificação Triazinas/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Herbicides); 0 (Triazines); 1SPQ95183Y (ametryne); 3KX376GY7L (Glutamic Acid); 7440-44-0 (Carbon); 9DLQ4CIU6V (Proline); N762921K75 (Nitrogen); QJA9M5H4IM (Atrazine) [Em] Mês de entrada: 1803 [Cu] Atualização por classe: 180308 [Lr] Data última revisão: 180308 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171009 [St] Status: MEDLINE

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[PMID]: 29324804 [Au] Autor: Santiago TR; Pereira VM; de Souza WR; Steindorff AS; Cunha BADB; Gaspar M; Fávaro LCL; Formighieri EF; Kobayashi AK; C Molinari HB [Ad] Endereço: Embrapa Agroenergia. Parque Estação Biológica, Av. W3 Norte (final), Asa Norte, Brasília, DF, Brazil. [Ti] Título: Genome-wide identification, characterization and expression profile analysis of expansins gene family in sugarcane (Saccharum spp.). [So] Source: PLoS One;13(1):e0191081, 2018. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Expansins refer to a family of closely related non-enzymatic proteins found in the plant cell wall that are involved in the cell wall loosening. In addition, expansins appear to be involved in different physiological and environmental responses in plants such as leaf and stem initiation and growth, stomata opening and closing, reproduction, ripening and stress tolerance. Sugarcane (Saccharum spp.) is one of the main crops grown worldwide. Lignocellulosic biomass from sugarcane is one of the most promising raw materials for the ethanol industry. However, the efficient use of lignocellulosic biomass requires the optimization of several steps, including the access of some enzymes to the hemicellulosic matrix. The addition of expansins in an enzymatic cocktail or their genetic manipulation could drastically improve the saccharification process of feedstock biomass by weakening the hydrogen bonds between polysaccharides present in plant cell walls. In this study, the expansin gene family in sugarcane was identified and characterized by in silico analysis. Ninety two putative expansins in sugarcane (SacEXPs) were categorized in three subfamilies after phylogenetic analysis. The expression profile of some expansin genes in leaves of sugarcane in different developmental stages was also investigated. This study intended to provide suitable expansin targets for genetic manipulation of sugarcane aiming at biomass and yield improvement. [Mh] Termos MeSH primário: Perfilação da Expressão Gênica Genes de Plantas Saccharum/genética [Mh] Termos MeSH secundário: Biomassa Ligações de Hidrogênio [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180223 [Lr] Data última revisão: 180223 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180112 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0191081

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[PMID]: 29250551 [Au] Autor: Naspolini BF; Machado ACO; Cravo Junior WB; Freire DMG; Cammarota MC [Ad] Endereço: National Institute of Technology (INT-MCTIC), Av. Venezuela, No. 82, Centro, 22453-900 Rio de Janeiro, RJ, Brazil. [Ti] Título: Bioconversion of Sugarcane Vinasse into High-Added Value Products and Energy. [So] Source: Biomed Res Int;2017:8986165, 2017. [Is] ISSN: 2314-6141 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Vinasse, a residue from bioethanol production containing high organic matter concentration, was used as substrate in submerged fermentation of PA1 for biosurfactant production. About 2.7 g/L of rhamnolipids was obtained, with surface tension of 29.2 mN/m and critical micelle concentration of 80.3 mg/L. After separation of rhamnolipid and biomass, residual fermentation media were submitted to anaerobic biodegradation in mesophilic conditions. The residual medium derived from fermentation with vinasse diluted to 1 : 1, without addition of nitrogen, C : N 21, and for 168 h, led to 63.2% chemical oxygen demand (COD) removal and 97.6 mL CH /g COD . Compared to results obtained with fresh vinasse (73.7% COD removal and 112.4 mL CH /g COD ), it could be concluded that both processes can be integrated in order to add value to the residue and obtain energy, reducing production costs and at the same time environmental impacts related to vinasse disposal. [Mh] Termos MeSH primário: Biocombustíveis Reatores Biológicos Saccharum/química [Mh] Termos MeSH secundário: Análise da Demanda Biológica de Oxigênio Biomassa Fermentação Glicerol/metabolismo Glicolipídeos/metabolismo Nitrogênio/metabolismo Pseudomonas aeruginosa/metabolismo Tensoativos/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biofuels); 0 (Glycolipids); 0 (Surface-Active Agents); 0 (rhamnolipid); N762921K75 (Nitrogen); PDC6A3C0OX (Glycerol) [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180129 [Lr] Data última revisão: 180129 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171219 [St] Status: MEDLINE [do] DOI: 10.1155/2017/8986165

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[PMID]: 29228055 [Au] Autor: Pereira-Santana A; Alvarado-Robledo EJ; Zamora-Briseño JA; Ayala-Sumuano JT; Gonzalez-Mendoza VM; Espadas-Gil F; Alcaraz LD; Castaño E; Keb-Llanes MA; Sanchez-Teyer F; Rodriguez-Zapata LC [Ad] Endereço: Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Mérida, Yucatán, México. [Ti] Título: Transcriptional profiling of sugarcane leaves and roots under progressive osmotic stress reveals a regulated coordination of gene expression in a spatiotemporal manner. [So] Source: PLoS One;12(12):e0189271, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Sugarcane is one of the most important crops worldwide and is a key plant for the global production of sucrose. Sugarcane cultivation is severely affected by drought stress and it is considered as the major limiting factor for their productivity. In recent years, this plant has been subjected to intensive research focused on improving its resilience against water scarcity; particularly the molecular mechanisms in response to drought stress have become an underlying issue for its improvement. To better understand water stress and the molecular mechanisms we performed a de novo transcriptomic assembly of sugarcane (var. Mex 69-290). A total of 16 libraries were sequenced in a 2x100 bp configuration on a HiSeq-Illumina platform. A total of 536 and 750 genes were differentially up-regulated along with the stress treatments for leave and root tissues respectively, while 1093 and 531 genes were differentially down-regulated in leaves and roots respectively. Gene Ontology functional analysis showed that genes related to response of water deprivation, heat, abscisic acid, and flavonoid biosynthesis were enriched during stress treatment in our study. The reliability of the observed expression patterns was confirmed by RT-qPCR. Additionally, several physiological parameters of sugarcane were significantly affected due to stress imposition. The results of this study may help identify useful target genes and provide tissue-specific data set of genes that are differentially expressed in response to osmotic stress, as well as a complete analysis of the main groups is significantly enriched under this condition. This study provides a useful benchmark for improving drought tolerance in sugarcane and other economically important grass species. [Mh] Termos MeSH primário: Perfilação da Expressão Gênica Saccharum/genética Transcrição Genética [Mh] Termos MeSH secundário: Pressão Osmótica Folhas de Planta/metabolismo Raízes de Plantas/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1801 [Cu] Atualização por classe: 180104 [Lr] Data última revisão: 180104 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 171212 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0189271

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[PMID]: 28873513 [Au] Autor: You X; van Heiningen A; Sixta H; Iakovlev M [Ad] Endereço: Department of Bioproducts and Biosystem, Aalto University, FI-00076 Aalto, Finland. Electronic address: Xiang.you@aalto.fi. [Ti] Título: Lignin and ash balances of sulfur dioxide-ethanol-water fractionation of sugarcane straw. [So] Source: Bioresour Technol;244(Pt 1):1111-1120, 2017 Nov. [Is] ISSN: 1873-2976 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Lignin and ash material balances of SO -ethanol-water (AVAP®) fractionation of sugarcane (SC) straw were thoroughly studied at various conditions. Most of straw lignin and ash dissolve in the liquor and 40-80% of lignin is precipitated after ethanol removal as a pure (∼99%) and sulfur-lean (<2%) fraction. Most of the acid-soluble ash and its elements (Na, K, Fe, Al) as well as large portion of silica are removed from the fiber phase. Straw lignin behavior exhibited differences compared to wood lignin including high apparent content in fiber, higher degree of sulfonation of dissolved lignin, and dense char-like precipitate formation upon ethanol removal. Variation in fractionation conditions did not have significant effect on lignin properties, while post-sulfonation was capable of changing its form from char-like to colloidal precipitate. [Mh] Termos MeSH primário: Etanol Saccharum Dióxido de Enxofre [Mh] Termos MeSH secundário: Fracionamento Químico Lignina Água [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 059QF0KO0R (Water); 0UZA3422Q4 (Sulfur Dioxide); 3K9958V90M (Ethanol); 9005-53-2 (Lignin) [Em] Mês de entrada: 1711 [Cu] Atualização por classe: 171103 [Lr] Data última revisão: 171103 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170907 [St] Status: MEDLINE

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[PMID]: 28855118 [Au] Autor: da Silva MD; de Oliveira Silva RL; Ferreira Neto JRC; Benko-Iseppon AM; Kido EA [Ad] Endereço: Federal University of Pernambuco (UFPE), Bioscience Center, Department of Genetics, 50670-420 Recife, PE, Brazil. [Ti] Título: Genotype-dependent regulation of drought-responsive genes in tolerant and sensitive sugarcane cultivars. [So] Source: Gene;633:17-27, 2017 Oct 30. [Is] ISSN: 1879-0038 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Drought is the most damaging among the major abiotic stresses. Transcriptomic studies allow a global overview of expressed genes, providing the basis for molecular markers development. Here, the HT-SuperSAGE technique allowed the evaluation of four drought-tolerant cultivars and four-sensitive cultivars, after 24h of irrigation suppression. We identified 9831 induced unitags from roots of the tolerant cultivars with different regulations by the -sensitive cultivars after the applied stress. These unitags allowed a proposal of 15 genes, whose expressed profiles were validated by RT-qPCR, evaluating each cultivar independently. These genes covered broad metabolic processes: ethylene stress attenuation (ACCD); root growth (ß-EXP8); protein degradation [ubiquitination pathway (E2, 20SPß4); plant proteases (AP, C13)]; oxidative detoxification (TRX); fatty acid synthesis (ACC); amino acid transport (AAT), and carbohydrate metabolism [glycolysis (PFK, TPI, FBA); TCA cycle (LDP, MDH); pentose phosphate pathway (TKT)]. The expressed profiles showed a genotype-dependent regulation of the target genes. Two drought-tolerant cultivars (SP83-2847; CTC6) presented each one, nine of the induced genes. Among the -sensitive cultivars, CTC13 induced only one, while SP90-1636 induced two genes. These genes should help breeders to identify accessions managing drought stress tolerance responses, showing better ethylene stress attenuation, energy allocation, amino acid transport, and protein homeostasis. [Mh] Termos MeSH primário: Secas Regulação da Expressão Gênica de Plantas Saccharum/genética Estresse Fisiológico/genética [Mh] Termos MeSH secundário: Etilenos/metabolismo Perfilação da Expressão Gênica Biblioteca Gênica Genes de Plantas Genótipo Glicólise/genética Glicólise/fisiologia Melhoramento Vegetal Proteínas de Plantas/genética Proteínas de Plantas/metabolismo Raízes de Plantas/genética Raízes de Plantas/metabolismo RNA/genética Reação em Cadeia da Polimerase em Tempo Real Saccharum/metabolismo Proteases Específicas de Ubiquitina/genética Proteases Específicas de Ubiquitina/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Ethylenes); 0 (Plant Proteins); 0 (expansin protein, plant); 63231-63-0 (RNA); 91GW059KN7 (ethylene); EC 3.4.19.12 (Ubiquitin-Specific Proteases) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170929 [Lr] Data última revisão: 170929 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170901 [St] Status: MEDLINE

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[PMID]: 28851160 [Au] Autor: Chang M; Li D; Wang W; Chen D; Zhang Y; Hu H; Ye X [Ad] Endereço: College of Materials Science and Energy Engineering, Foshan University, Foshan, Guangdong 528000, PR China. [Ti] Título: Comparison of sodium hydroxide and calcium hydroxide pretreatments on the enzymatic hydrolysis and lignin recovery of sugarcane bagasse. [So] Source: Bioresour Technol;244(Pt 1):1055-1058, 2017 Nov. [Is] ISSN: 1873-2976 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Sodium hydroxide (NaOH) and calcium hydroxide (Ca(OH) ) respectively dissolved in water and 70% glycerol were applied to treat sugarcane bagasse (SCB) under the condition of 80°C for 2h. NaOH solutions could remove more lignin and obtain higher enzymatic hydrolysis efficiency of SCB than Ca(OH) solutions. Compared with the alkali-water solutions, the enzymatic hydrolysis of SCB treated in NaOH-glycerol solution decreased, while that in Ca(OH) -glycerol solution increased. The lignin in NaOH-water pretreatment liquor could be easily recovered by calcium chloride (CaCl ) at room temperature, but that in Ca(OH) -water pretreatment liquor couldn't. NaOH pretreatment is more suitable for facilitating enzymatic hydrolysis and lignin recovery of SCB than Ca(OH) pretreatment. [Mh] Termos MeSH primário: Hidróxido de Cálcio Celulose Lignina Hidróxido de Sódio [Mh] Termos MeSH secundário: Hidrólise Saccharum [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 55X04QC32I (Sodium Hydroxide); 9004-34-6 (Cellulose); 9005-53-2 (Lignin); 9006-97-7 (bagasse); PF5DZW74VN (Calcium Hydroxide) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171025 [Lr] Data última revisão: 171025 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170831 [St] Status: MEDLINE

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[PMID]: 28821913 [Au] Autor: Wang HB; Chen PH; Yang YQ; D'Hont A; Lu YH [Ad] Endereço: Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Key Laboratory of Ministry of Agriculture for Sugarcane Biology and Genetic Breeding, College of Crop Science, Fujian Agriculture and Forestry University, Jinshan, Fuzhou, 350002, People's Republic of C [Ti] Título: Molecular insights into the origin of the brown rust resistance gene Bru1 among Saccharum species. [So] Source: Theor Appl Genet;130(11):2431-2443, 2017 Nov. [Is] ISSN: 1432-2242 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: KEY MESSAGE: Analysis of 387 sugarcane clones using Bru 1 diagnostic markers revealed two possible sources of Bru 1 in Chinese cultivars: one from Saccharum spontaneum and another from Saccharum robustum of New Guinea. Sugarcane brown rust (SBR) is an important fungal disease in many sugarcane production areas around the world, and can cause considerable yield losses in susceptible sugarcane cultivars. One major SBR resistance gene, named Bru1, initially identified from cultivar R570, was shown to be a major SBR resistance source in most of the sugarcane producing areas of the world. In this study, by using the two Bru1-associated markers, R12H16 and 9O20-F4, we surveyed the presence of Bru1 in a Chinese sugarcane germplasm collection of 387 clones, consisting of 228 hybrid cultivars bred by different Chinese sugarcane breeding establishments, 54 exotic hybrid cultivars introduced from other countries and 105 clones of sugarcane ancestral species. The Bru1-bearing haplotype was detected in 43.4% of Chinese sugarcane cultivars, 20.4% of exotic hybrid cultivars, and only 3.8% of ancestral species. Among the 33 Chinese cultivars for which phenotypes of resistance to SBR were available, Bru1 was present in 69.2% (18/26) of the resistant clones. Analyses of the allelic sequence variations of R12H16 and 9O20-F4 suggested two possible sources of Bru1 in Chinese cultivars: one from S. spontaneum and another from S. robustum of New Guinea. In addition, we developed an improved Bru1 diagnostic marker, 9O20-F4-HaeIII, which can eliminate all the false results of 9O20-F4-RsaI observed among S. spontaneum, as well as a new dominant Bru1 diagnostic marker, R12E03-2, from the BAC ShCIR12E03. Our results provide valuable information for further efforts of breeding SBR-resistant varieties, searching new SBR resistance sources and cloning of Bru1 in sugarcane. [Mh] Termos MeSH primário: Basidiomycota Resistência à Doença/genética Genes de Plantas Doenças das Plantas/genética Saccharum/genética [Mh] Termos MeSH secundário: Alelos Sequência de Bases Marcadores Genéticos Haplótipos Hibridização Genética Fenótipo Filogenia Doenças das Plantas/microbiologia Saccharum/microbiologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Genetic Markers) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171018 [Lr] Data última revisão: 171018 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170820 [St] Status: MEDLINE [do] DOI: 10.1007/s00122-017-2968-3

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[PMID]: 28817735 [Au] Autor: Hoang NV; Furtado A; O'Keeffe AJ; Botha FC; Henry RJ [Ad] Endereço: Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, St. Lucia, Queensland, Australia. [Ti] Título: Association of gene expression with biomass content and composition in sugarcane. [So] Source: PLoS One;12(8):e0183417, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: About 64% of the total aboveground biomass in sugarcane production is from the culm, of which ~90% is present in fiber and sugars. Understanding the transcriptome in the sugarcane culm, and the transcripts that are associated with the accumulation of the sugar and fiber components would facilitate the modification of biomass composition for enhanced biofuel and biomaterial production. The Sugarcane Iso-Seq Transcriptome (SUGIT) database was used as a reference for RNA-Seq analysis of variation in gene expression between young and mature tissues, and between 10 genotypes with varying fiber content. Global expression analysis suggests that each genotype displayed a unique expression pattern, possibly due to different chromosome combinations and maturation amongst these genotypes. Apart from direct sugar- and fiber-related transcripts, the differentially expressed (DE) transcripts in this study belonged to various supporting pathways that are not obviously involved in the accumulation of these major biomass components. The analysis revealed 1,649 DE transcripts between the young and mature tissues, while 555 DE transcripts were found between the low and high fiber genotypes. Of these, 151 and 23 transcripts respectively, were directly involved in sugar and fiber accumulation. Most of the transcripts identified were up-regulated in the young tissues (2 to 22-fold, FDR adjusted p-value <0.05), which could be explained by the more active metabolism in the young tissues compared to the mature tissues in the sugarcane culm. The results of analysis of the contrasting genotypes suggests that due to the large number of genes contributing to these traits, some of the critical DE transcripts could display less than 2-fold differences in expression and might not be easily identified. However, this transcript profiling analysis identified full-length candidate transcripts and pathways that were likely to determine the differences in sugar and fiber accumulation between tissue types and contrasting genotypes. [Mh] Termos MeSH primário: Biomassa Regulação da Expressão Gênica de Plantas Saccharum/genética [Mh] Termos MeSH secundário: RNA Mensageiro/genética RNA de Plantas/genética Saccharum/metabolismo Análise de Sequência de RNA [Pt] Tipo de publicação: JOURNAL ARTICLE; VALIDATION STUDIES [Nm] Nome de substância: 0 (RNA, Messenger); 0 (RNA, Plant) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171013 [Lr] Data última revisão: 171013 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170818 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0183417

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[PMID]: 28817651 [Au] Autor: Huang Y; Yu F; Li X; Luo L; Wu J; Yang Y; Deng Z; Chen R; Zhang M [Ad] Endereço: Key Lab of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry University, Fuzhou, China. [Ti] Título: Comparative genetic analysis of the 45S rDNA intergenic spacers from three Saccharum species. [So] Source: PLoS One;12(8):e0183447, 2017. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The 45S ribosomal DNA (rDNA) units are separated by an intergenic spacer (IGS) containing the signals for transcription and processing of rRNAs. For the first time, we sequenced and analyzed the entire IGS region from three original species within the genus Saccharum, including S. spontaneum, S. robustum, and S. officinarum in this study. We have compared the IGS organization within three original species of the genus Saccharum. The IGS of these three original species showed similar overall organizations comprised of putative functional elements needed for rRNA gene activity as well as a non-transcribed spacer (NTS), a promoter region, and an external transcribed spacer (ETS). The variability in length of the IGS sequences was assessed at the individual, intraspecies, and interspecies levels of the genus Saccharum, including S. spontaneum, S. robustum, and S. officinarum. The ETS had greater similarity than the NTS across species, but nevertheless exhibited variation in length. Within the IGS of the Saccharum species, base substitutions and copy number variation of sub-repeat were causes of the divergence in IGS sequences. We also identified a significant number of methylation sites. Furthermore, fluorescent in situ hybridization (FISH) co-localization of IGS and pTa71 probes was detected on all representative species of the genus Saccharum tested. Taken together, the results of this study provide a better insight into the structure and organization of the IGS in the genus Saccharum. [Mh] Termos MeSH primário: DNA Ribossômico/genética Saccharum/genética [Mh] Termos MeSH secundário: Sequência de Bases Ilhas de CpG Metilação de DNA DNA de Plantas/genética Hibridização in Situ Fluorescente Regiões Promotoras Genéticas RNA Mensageiro/genética Saccharum/classificação Homologia de Sequência do Ácido Nucleico Especificidade da Espécie [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Plant); 0 (DNA, Ribosomal); 0 (RNA, Messenger) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171013 [Lr] Data última revisão: 171013 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170818 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0183447

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