Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.822.844 [Categoria DeCS]
Referências encontradas : 323 [refinar]
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  1 / 323 MEDLINE  
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[PMID]:28450660
[Au] Autor:Kong B; Chen L; Kasahara Y; Sumida A; Ono K; Wild J; Nagatake A; Hatano R; Hara T
[Ad] Endereço:Institute of Low Temperature Science, Hokkaido University.
[Ti] Título:Understory Dwarf Bamboo Affects Microbial Community Structures and Soil Properties in a Betula ermanii Forest in Northern Japan.
[So] Source:Microbes Environ;32(2):103-111, 2017 Jun 24.
[Is] ISSN:1347-4405
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:In order to understand the relationships between understory bamboo and soil properties, we compared microbial community structures in the soil of a Betula ermanii boreal forest with Sasa kurilensis present and removed using high-throughput DNA sequencing. The presence of understory S. kurilensis strongly affected soil properties, including total carbon, total nitrogen, nitrate, and the C:N ratio as well as relative soil moisture. Marked differences were also noted in fungal and bacterial communities between plots. The relative abundance of the fungal phylum Ascomycota was 13.9% in the Sasa-intact plot and only 0.54% in the Sasa-removed plot. Among the Ascomycota fungi identified, the most prevalent were members of the family Pezizaceae. We found that the abundance of Pezizaceae, known to act as mycorrhizal fungi, was related to the amount of total carbon in the Sasa-intact plot. The relative abundance of Proteobacteria was significantly higher, whereas those of Planctomycetes and Actinobacteria were lower in the Sasa-intact plot than in the Sasa-removed plot. Furthermore, the results obtained suggest that some species of the phylum Planctomycetes are more likely to occur in the presence of S. kurilensis. Collectively, these results indicate that the presence of S. kurilensis affects microbial communities and soil properties in a B. ermanii boreal forest.
[Mh] Termos MeSH primário: Betula
Florestas
Sasa/crescimento & desenvolvimento
Microbiologia do Solo
[Mh] Termos MeSH secundário: Bactérias/classificação
Carbono/análise
Fungos/classificação
Sequenciamento de Nucleotídeos em Larga Escala
Japão
Nitrogênio/análise
Análise de Sequência de DNA
Solo/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil); 7440-44-0 (Carbon); N762921K75 (Nitrogen)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170429
[St] Status:MEDLINE
[do] DOI:10.1264/jsme2.ME16154


  2 / 323 MEDLINE  
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[PMID]:28822283
[Au] Autor:Chen TY; Wen JL; Wang B; Wang HM; Liu CF; Sun RC
[Ad] Endereço:Beijing Key Laboratory of Lignocellulosic Chemistry, Beijing Forestry University, Beijing 100083, China.
[Ti] Título:Assessment of integrated process based on autohydrolysis and robust delignification process for enzymatic saccharification of bamboo.
[So] Source:Bioresour Technol;244(Pt 1):717-725, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, bamboo (Phyllostachys pubescens) was successfully deconstructed using an integrated process (autohydrolysis and subsequent delignification). Xylooligosaccharides, high-purity lignin, and digestible substrates for producing glucose can be consecutively collected during the integrated process. The structural change and fate of lignin during autohydrolysis process was deeply investigated. Additionally, the structural characteristics and active functional groups of the lignin fractions obtained by these delignification processes were thoroughly investigated by NMR (2D-HSQC and P NMR) and GPC techniques. The chemical compositions (S, G, and H) and major linkages (ß-O-4, ß-ß, ß-5, etc.) were thoroughly assigned and the frequencies of the major lignin linkages were quantitatively compared. Considering the structural characteristics and molecular weights of the lignin as well as enzymatic saccharification ratio of the substrate, the combination of autohydrolysis and organic base-catalyzed ethanol pretreatment was deemed as a promising biorefinery mode in the future based on bamboo feedstock.
[Mh] Termos MeSH primário: Lignina
Sasa/química
[Mh] Termos MeSH secundário: Etanol
Hidrólise
Peso Molecular
Compostos Orgânicos
Sasa/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Organic Chemicals); 3K9958V90M (Ethanol); 9005-53-2 (Lignin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170820
[St] Status:MEDLINE


  3 / 323 MEDLINE  
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[PMID]:28726396
[Au] Autor:Yang JH; Choi MH; Yang SH; Cho SS; Park SJ; Shin HJ; Ki SH
[Ad] Endereço:College of Pharmacy and ‡Department of Biochemical and Polymer Engineering, Chosun University , Gwangju, 61452, Republic of Korea.
[Ti] Título:Potent Anti-Inflammatory and Antiadipogenic Properties of Bamboo (Sasa coreana Nakai) Leaves Extract and Its Major Constituent Flavonoids.
[So] Source:J Agric Food Chem;65(31):6665-6673, 2017 Aug 09.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The pro-inflammatory response and recruitment of macrophages into adipose tissue contribute to metabolic dysfunction. Here, we reported the anti-inflammatory and antiadipogenic effects of the methanol (MeOH) extract and ethyl acetate (EtOAc) fraction of bamboo leaf and its molecular mechanism in RAW264.7 cells and 3T3-L1 adipocytes, respectively. Functional macrophage migration assays also were performed. Surprisingly, the EtOAc fraction of MeOH extracts from native Korean plant species Sasa coreana Nakai (SCN) has shown potent anti-inflammatory properties; SCN pretreatment inhibited nitric oxide (NO) production (p < 0.01) and inducible nitric oxide synthase (iNOS) expression in lipopolysaccharide (LPS)-stimulated macrophages. Inflammatory genes induced by LPS, including TNFα, IL-1ß, and IL-6, were significantly attenuated by SCN (p < 0.01). Pretreatment with SCN antagonized NF-κB nuclear translocation and the simultaneous degradation of inhibitory κB protein. Furthermore, SCN selectively inhibited the LPS-induced phosphorylation of JNK (p < 0.01) and p38 (p < 0.05) but not ERK (p > 0.05). Similar to leaf extracts of other bamboo species, we identified that SCN contained several flavonoids including orientin, isoorientin, and vitexin; these compounds inhibited LPS-induced NO production (p < 0.05) and iNOS expression. In addition, SCN inhibited adipocyte differentiation in a dose-dependent manner, as demonstrated by Oil Red O staining and the protein expression of mature adipogenic marker genes. Treatment with the major flavonoids of SCN also inhibited adipogenesis. Furthermore, conditioned medium obtained from adipocytes stimulated macrophage chemotaxis, whereas medium from adipocytes treated with SCN significantly inhibited macrophage migration. Therefore, SCN is a potential therapeutic agent for the prevention of inflammation and obesity.
[Mh] Termos MeSH primário: Adipócitos/efeitos dos fármacos
Adipogenia/efeitos dos fármacos
Anti-Inflamatórios/farmacologia
Flavonoides/farmacologia
Extratos Vegetais/farmacologia
Sasa/química
[Mh] Termos MeSH secundário: Células 3T3
Adipócitos/citologia
Animais
Anti-Inflamatórios/química
Anti-Inflamatórios/isolamento & purificação
Flavonoides/química
Flavonoides/isolamento & purificação
Interleucina-6/genética
Interleucina-6/imunologia
Macrófagos/efeitos dos fármacos
Macrófagos/imunologia
Camundongos
NF-kappa B/genética
NF-kappa B/imunologia
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Folhas de Planta/química
Células RAW 264.7
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Flavonoids); 0 (Interleukin-6); 0 (NF-kappa B); 0 (Plant Extracts); 0 (Tumor Necrosis Factor-alpha)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02203


  4 / 323 MEDLINE  
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[PMID]:28633601
[Au] Autor:Yagi H; Tateno H; Hayashi K; Hayashi T; Takahashi K; Hirabayashi J; Kato K; Tsuboi M
[Ad] Endereço:a Graduate School of Pharmaceutical Sciences , Nagoya City University , Nagoya , Japan.
[Ti] Título:Lectin microarray analysis of isolated polysaccharides from Sasa veitchii.
[So] Source:Biosci Biotechnol Biochem;81(9):1687-1689, 2017 Sep.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We report lectin microarray profile of the polysaccharide fraction derived from Sasa veitchii leaf that exhibits anti-influenza activity. This fraction showed higher reactivities with lectins known as binders to oligo-mannose, fucose, or galactose. Our findings along with previously reported monosaccharide components suggest that the polysaccharide can be cross-reactive with cell surface receptors involved in immune system, thereby exerting anti-influenza activity.
[Mh] Termos MeSH primário: Antivirais/metabolismo
Lectinas/metabolismo
Polissacarídeos/metabolismo
Análise Serial de Proteínas
Sasa/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Lectins); 0 (Polysaccharides)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170622
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1340089


  5 / 323 MEDLINE  
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[PMID]:28439758
[Au] Autor:Ghosh JS; Bhattacharya S; Pal A
[Ad] Endereço:Division of Plant Biology, Bose Institute, P1/12 CIT Scheme VIIM, Kolkata, 700054, India.
[Ti] Título:Molecular phylogeny of 21 tropical bamboo species reconstructed by integrating non-coding internal transcribed spacer (ITS1 and 2) sequences and their consensus secondary structure.
[So] Source:Genetica;145(3):319-333, 2017 Jun.
[Is] ISSN:1573-6857
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The unavailability of the reproductive structure and unpredictability of vegetative characters for the identification and phylogenetic study of bamboo prompted the application of molecular techniques for greater resolution and consensus. We first employed internal transcribed spacer (ITS1, 5.8S rRNA and ITS2) sequences to construct the phylogenetic tree of 21 tropical bamboo species. While the sequence alone could grossly reconstruct the traditional phylogeny amongst the 21-tropical species studied, some anomalies were encountered that prompted a further refinement of the phylogenetic analyses. Therefore, we integrated the secondary structure of the ITS sequences to derive individual sequence-structure matrix to gain more resolution on the phylogenetic reconstruction. The results showed that ITS sequence-structure is the reliable alternative to the conventional phenotypic method for the identification of bamboo species. The best-fit topology obtained by the sequence-structure based phylogeny over the sole sequence based one underscores closer clustering of all the studied Bambusa species (Sub-tribe Bambusinae), while Melocanna baccifera, which belongs to Sub-Tribe Melocanneae, disjointedly clustered as an out-group within the consensus phylogenetic tree. In this study, we demonstrated the dependability of the combined (ITS sequence+structure-based) approach over the only sequence-based analysis for phylogenetic relationship assessment of bamboo.
[Mh] Termos MeSH primário: Filogenia
RNA não Traduzido/genética
Sasa/genética
[Mh] Termos MeSH secundário: Conformação de Ácido Nucleico
RNA não Traduzido/química
Sasa/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Untranslated)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170426
[St] Status:MEDLINE
[do] DOI:10.1007/s10709-017-9967-9


  6 / 323 MEDLINE  
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[PMID]:28314260
[Au] Autor:Sakagami H; Okudaira N; Masuda Y; Amano O; Yokose S; Kanda Y; Suguro M; Natori T; Oizumi H; Oizumi T
[Ad] Endereço:Division of Pharmacology, Meikai University School of Dentistry, Sakado, Japan sakagami@dent.meikai.ac.jp takaakio@daiwaseibutsu.co.jp.
[Ti] Título:Induction of Apoptosis in Human Oral Keratinocyte by Doxorubicin.
[So] Source:Anticancer Res;37(3):1023-1029, 2017 03.
[Is] ISSN:1791-7530
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:BACKGROUND/AIM: We have previously reported that doxorubicin (DXR) showed much higher cytotoxicity against human oral squamous cell carcinoma cell lines compared to normal human mesenchymal normal oral cells (gingival fibroblast, periodontal ligament fibroblast, pulp cell), yielding high tumor-specificity. However, we unexpectedly found that doxorubicin showed potent cytotoxicity against human normal oral keratinocytes and primary gingival epithelial cells. In the present study, we investigated the reproducibility, underlining mechanisms and generality of this unexpected finding. MATERIALS AND METHODS: Viable cell number was determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method, fine cell structure by transmission electron microscopy and apoptosis induction by western blot analysis. RESULTS: Doxorubicin induced keratinocyte toxicity, regardless of cell density and concentration of FBS in the culture medium. Doxorubicin induced apoptosis (characterized by the loss of cell surface microvilli, chromatin condensation, nuclear fragmentation and caspase-3 activation) in keratinocytes. A total of 11 anticancer drugs showed similar keratinocyte toxicity. Alkaline extract of the leaves of Sasa senanensis Rehder partially alleviated the DXR-induced keratinocyte cytotoxicity by promoting cell growth. CONCLUSION: The present study suggested that oral keratinocyte toxicity is a novel adverse effect of most anticancer agents.
[Mh] Termos MeSH primário: Antineoplásicos/efeitos adversos
Apoptose
Doxorrubicina/efeitos adversos
Queratinócitos/patologia
[Mh] Termos MeSH secundário: Caspase 3/metabolismo
Adesão Celular
Linhagem Celular Tumoral
Cromatina/metabolismo
Fibroblastos/efeitos dos fármacos
Fibroblastos/patologia
Gengiva/citologia
Seres Humanos
Queratinócitos/citologia
Queratinócitos/efeitos dos fármacos
Microscopia Eletrônica de Transmissão
Neoplasias Bucais/patologia
Ligamento Periodontal/citologia
Extratos Vegetais/química
Folhas de Planta/química
Sasa/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Chromatin); 0 (Plant Extracts); 80168379AG (Doxorubicin); EC 3.4.22.- (CASP3 protein, human); EC 3.4.22.- (Caspase 3)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170411
[Lr] Data última revisão:
170411
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170319
[St] Status:MEDLINE


  7 / 323 MEDLINE  
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[PMID]:28281076
[Au] Autor:Li Y; Swaisgood RR; Wei W; Nie Y; Hu Y; Yang X; Gu X; Zhang Z
[Ad] Endereço:Key Laboratory of Southwest China Wildlife Resources Conservation, Ministry of Education, Nanchong, China.
[Ti] Título:Withered on the stem: is bamboo a seasonally limiting resource for giant pandas?
[So] Source:Environ Sci Pollut Res Int;24(11):10537-10546, 2017 Apr.
[Is] ISSN:1614-7499
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:In response to seasonal variation in quality and quantity of available plant biomass, herbivorous foragers may alternate among different plant resources to meet nutritional requirements. Giant pandas (Ailuropoda melanoleuca) are reliant almost exclusively on bamboo which appears omnipresent in most occupied habitat, but subtle temporal variation in bamboo quality may still govern foraging strategies, with population-level effects. In this paper, we investigated the possibility that temporal variation in the quality of this resource is involved in population regulation and examined pandas' adaptive foraging strategies in response to temporal variation in bamboo quality. Giant pandas in late winter and early spring consumed a less optimal diet in Foping Nature Reserve, as the availability of the most nutritious and preferred components and age classes of Bashania fargesii declined, suggesting that bamboo may be a seasonally limiting resource. Most panda mortalities and rescues occurred during the same period of seasonal food limitation. Our findings raised the possibility that while total bamboo biomass may not be a limiting factor, carrying capacity may be influenced by subtle seasonal variation in bamboo quality. We recommend that managers and policy-makers should consider more than just the quantity of bamboo in the understory and that carrying capacity estimates should be revised downward to reflect the fact that all bamboos are not equal.
[Mh] Termos MeSH primário: Conservação dos Recursos Naturais
Ursidae
[Mh] Termos MeSH secundário: Animais
Ecossistema
Comportamento Alimentar
Sasa
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.1007/s11356-017-8746-6


  8 / 323 MEDLINE  
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[PMID]:28222348
[Au] Autor:Li H; Duan M; Gu J; Zhang Y; Qian X; Ma J; Zhang R; Wang X
[Ad] Endereço:College of Natural Resources and Environment, Northwest A&F University, Yangling, Shaanxi 712100, People's Republic of China.
[Ti] Título:Effects of bamboo charcoal on antibiotic resistance genes during chicken manure composting.
[So] Source:Ecotoxicol Environ Saf;140:1-6, 2017 Jun.
[Is] ISSN:1090-2414
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Composting is widely used for animal waste disposal, and bamboo charcoal (BC) can be used for nitrogen conservation during composting. However, the effects of BC on antibiotic resistance genes (ARGs) during chicken manure composting are still unclear. This study investigated the effects on ARGs of adding different proportions of BC (0%, 5%, 10%, and 20% w/w) to chicken manure compost. After 26 days, the relative abundances (RAs) of most ARGs (tetC, tetG, tetW, tetX, sul2, drfA1, drfA7, ermB, ermF, ermQ, and ermX) and intI1 declined by 21.6-99.5%, whereas sul1 increased by 7.5-17.7 times. The average RAs reductions with 0%, 5%, 10%, and 20% BC were 0.85, 1.05, 1.08, and 1.15 logs, respectively. The most important environmental factor for the ARG profiles was temperature according to redundancy analysis. Furthermore, BC significantly decreased the bio-Cu and bio-Zn levels, thereby reducing the co-selection pressure from heavy metals. Different proportions of BC had no significant effects on the removal of tetG, tetW, tetX, sul2, drfA1, and ermB. Supplementation with 10% BC was more effective at removing tetC and drfA7 compared with the other treatments. The results suggested that 10% BC supplementation is appropriate for reducing ARGs in chicken manure compost.
[Mh] Termos MeSH primário: Antibacterianos/análise
Carvão Vegetal/química
Resistência Microbiana a Medicamentos/genética
Genes Bacterianos
Esterco/análise
Microbiologia do Solo
Solo/química
[Mh] Termos MeSH secundário: Animais
Galinhas
Metais Pesados/análise
Nitrogênio/análise
Eliminação de Resíduos
Sasa/crescimento & desenvolvimento
Solo/normas
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Manure); 0 (Metals, Heavy); 0 (Soil); 16291-96-6 (Charcoal); N762921K75 (Nitrogen)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170222
[St] Status:MEDLINE


  9 / 323 MEDLINE  
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[PMID]:28208644
[Au] Autor:Jia J; Zhang W; Yang Z; Yang X; Wang N; Yu X
[Ad] Endereço:Key Laboratory of Green Chemistry Technology, Ministry of Education, College of Chemistry, Sichuan University, Chengdu 610064, China. scujunqi@163.com.
[Ti] Título:Novel Magnetic Cross-Linked Cellulase Aggregates with a Potential Application in Lignocellulosic Biomass Bioconversion.
[So] Source:Molecules;22(2), 2017 Feb 10.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The utilization of renewable biomass resources to produce high-value chemicals by enzymatic processes is beneficial for alternative energy production, due to the accelerating depletion of fossil fuels. As immobilization techniques can improve enzyme stability and reusability, a novel magnetic cross-linked cellulase aggregate has been developed and applied for biomass bioconversion. The crosslinked aggregates could purify and immobilize enzymes in a single operation, and could then be combined with magnetic nanoparticles (MNPs), which provides easy separation of the materials. The immobilized cellulase showed a better activity at a wider temperature range and pH values than that of the free cellulase. After six cycles of consecutive reuse, the immobilized cellulase performed successful magnetic separation and retained 74% of its initial activity when carboxylmethyl cellulose (CMC) was used as the model substrate. Furthermore, the structure and morphology of the immobilized cellulase were studied by Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). Moreover, the immobilized cellulase was shown to hydrolyze bamboo biomass with a yield of 21%, and was re-used in biomass conversion up to four cycles with 38% activity retention, which indicated that the immobilized enzyme has good potential for biomass applications.
[Mh] Termos MeSH primário: Celulase/química
Enzimas Imobilizadas/química
Proteínas Fúngicas/química
Lignina/química
[Mh] Termos MeSH secundário: Reagentes para Ligações Cruzadas/química
Estabilidade Enzimática
Glutaral/química
Concentração de Íons de Hidrogênio
Hidrólise
Sasa/química
Trichoderma/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cross-Linking Reagents); 0 (Enzymes, Immobilized); 0 (Fungal Proteins); 11132-73-3 (lignocellulose); 9005-53-2 (Lignin); EC 3.2.1.4 (Cellulase); T3C89M417N (Glutaral)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170605
[Lr] Data última revisão:
170605
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170218
[St] Status:MEDLINE


  10 / 323 MEDLINE  
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[PMID]:28189615
[Au] Autor:Zhou MB; Hu H; Miskey C; Lazarow K; Ivics Z; Kunze R; Yang G; Izsvák Z; Tang DQ
[Ad] Endereço:The Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, LinAn, China.
[Ti] Título:Transposition of the bamboo Mariner-like element Ppmar1 in yeast.
[So] Source:Mol Phylogenet Evol;109:367-374, 2017 Apr.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The moso bamboo genome contains the two structurally intact and thus potentially functional mariner-like elements Ppmar1 and Ppmar2. Both elements contain perfect terminal inverted repeats (TIRs) and a full-length intact transposase gene. Here we investigated whether Ppmar1 is functional in yeast (Saccharomyces cerevisiae). We have designed a two-component system consisting of a transposase expression cassette and a non-autonomous transposon on two separate plasmids. We demonstrate that the Ppmar1 transposase Pptpase1 catalyses excision of the non-autonomous Ppmar1NA element from the plasmid and reintegration at TA dinucleotide sequences in the yeast chromosomes. In addition, we generated 14 hyperactive Ppmar1 transposase variants by systematic single amino acid substitutions. The most active transposase variant, S171A, induces 10-fold more frequent Ppmar1NA excisions in yeast than the wild type transposase. The Ppmar1 transposon is a promising tool for insertion mutagenesis in moso bamboo and may be used in other plants as an alternative to the established transposon tagging systems.
[Mh] Termos MeSH primário: Elementos de DNA Transponíveis
Saccharomyces cerevisiae/genética
Sasa/genética
[Mh] Termos MeSH secundário: DNA de Plantas
Filogenia
Plasmídeos
Transposases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Transposable Elements); 0 (DNA, Plant); EC 2.7.7.- (Transposases)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170927
[Lr] Data última revisão:
170927
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170213
[St] Status:MEDLINE



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