Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.825.333 [Categoria DeCS]
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[PMID]:29261741
[Au] Autor:Gao J; Wang T; Liu M; Liu J; Zhang Z
[Ad] Endereço:The Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, China.
[Ti] Título:Transcriptome analysis of filling stage seeds among three buckwheat species with emphasis on rutin accumulation.
[So] Source:PLoS One;12(12):e0189672, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Buckwheat is an important minor crop with pharmaceutical functions due to rutin enrichment in the seed. Seeds of common buckwheat cultivars (Fagopyrum esculentum, Fes) usually have much lower rutin content than tartary buckwheat (F. tartaricum, Ft). We previously found a wild species of common buckwheat (F. esculentum ssp. ancestrale, Fea), with seeds that are high in rutin, similar to Ft. In the present study, we investigated the mechanism by which rutin production varies among different buckwheat cultivars, Fea, a Ft variety (Xide) and a Fes variety (No.2 Pingqiao) using RNA sequencing of filling stage seeds. Sequencing data generated approximately 43.78-Gb of clean bases, all these data were pooled together and assembled 180,568 transcripts, and 109,952 unigenes. We established seed gene expression profiles of each buckwheat sample and assessed genes involved in flavonoid biosynthesis, storage proteins production, CYP450 family, starch and sucrose metabolism, and transcription factors. Differentially expressed genes between Fea and Fes were further analyzed due to their close relationship than with Ft. Expression levels of flavonoid biosynthesis gene FLS1 (Flavonol synthase 1) were similar in Fea and Ft, and much higher than in Fes, which was validated by qRT-PCR. This suggests that FLS1 transcript levels may be associated with rutin accumulation in filling stage seeds of buckwheat species. Further, we explored transcription factors by iTAK, and multiple gene families were identified as being involved in the coordinate regulation of metabolism and development. Our extensive transcriptomic data sets provide a complete description of metabolically related genes that are differentially expressed in filling stage buckwheat seeds and suggests that FLS1 is a key controller of rutin synthesis in buckwheat species. FLS1 can effectively convert dihydroflavonoids into flavonol products. These findings provide a basis for further studies of flavonoid biosynthesis in buckwheat breeding to help accelerate flavonoid metabolic engineering that would increase rutin content in cultivars of common buckwheat.
[Mh] Termos MeSH primário: Fagopyrum/embriologia
Fagopyrum/genética
Genes de Plantas
Rutina/metabolismo
Transcriptoma
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Fagopyrum/metabolismo
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Sementes/metabolismo
Análise de Sequência de RNA
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
5G06TVY3R7 (Rutin)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180116
[Lr] Data última revisão:
180116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0189672


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[PMID]:28460279
[Au] Autor:Gao F; Zhou J; Deng RY; Zhao HX; Li CL; Chen H; Suzuki T; Park SU; Wu Q
[Ad] Endereço:College of Life Science, Sichuan Agricultural University, No. 46, Xinkang Road, Ya'an, 625014 Sichuan Province, China.
[Ti] Título:Overexpression of a tartary buckwheat R2R3-MYB transcription factor gene, FtMYB9, enhances tolerance to drought and salt stresses in transgenic Arabidopsis.
[So] Source:J Plant Physiol;214:81-90, 2017 Jul.
[Is] ISSN:1618-1328
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Tartary buckwheat (Fagopyrum tataricum) is a traditional coarse cereal that exhibits strong plasticity in its adaptation to harsh and complicated environmental stresses. In an attempt to study the strong tolerance of tartary buckwheat, the FtMYB9 gene, which encodes an R2R3-MYB transcription factor protein, was functionally investigated. FtMYB9 expression was rapidly and strongly induced by ABA, cold, salt, and drought treatments in the seedling stage. A yeast one-hybrid system assay indicated that FtMYB9 is an activator of transcriptional activity, consistent with its roles as a transcription factor. Its overexpression in plants resulted in increased sensitivity to ABA at the germination and seedling stages compared to wild type. The overexpression of FtMYB9 increased tolerance to drought and salt stresses by the activation of some stress-related genes from both ABA-independent and ABA-dependent pathways in transgenic Arabidopsis. Furthermore, enhanced proline content and the activation of the P5CS1 gene implied that FtMYB9 may be involved in proline synthesis in plants. Collectively, these results suggest that FtMYB9 functions as a novel R2R3-MYB TF which plays positive roles in salt and drought tolerance by regulating different stress-responsive signaling pathways.
[Mh] Termos MeSH primário: Secas
Fagopyrum/metabolismo
Proteínas de Plantas/metabolismo
[Mh] Termos MeSH secundário: Ácido Abscísico/farmacologia
Arabidopsis/efeitos dos fármacos
Arabidopsis/genética
Arabidopsis/metabolismo
Fagopyrum/efeitos dos fármacos
Fagopyrum/genética
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Proteínas de Plantas/genética
Plantas Geneticamente Modificadas/efeitos dos fármacos
Plantas Geneticamente Modificadas/genética
Plantas Geneticamente Modificadas/metabolismo
Transdução de Sinais/efeitos dos fármacos
Transdução de Sinais/genética
Cloreto de Sódio/farmacologia
Fatores de Transcrição/genética
Fatores de Transcrição/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 0 (Transcription Factors); 451W47IQ8X (Sodium Chloride); 72S9A8J5GW (Abscisic Acid)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE


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[PMID]:27773388
[Au] Autor:Marín-Sáez J; Romero-González R; Garrido Frenich A
[Ad] Endereço:Department of Chemistry and Physics, Analytical Chemistry Area, University of Almería, Research Centre for Agricultural and Food Biotechnology (BITAL), Agrifood Campus of International Excellence ceiA3(,) Carretera de Sacramento s/n, E-04120, Almería, Spain.
[Ti] Título:Enantiomeric determination and evaluation of the racemization process of atropine in Solanaceae seeds and contaminated samples by high performance liquid chromatography-tandem mass spectrometry.
[So] Source:J Chromatogr A;1474:79-84, 2016 Nov 25.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:A new method has been developed for the enantioselective separation of (-) and (+) hyoscyamine in Solanaceaes seeds and contaminated buckwheat. Chromatographic separation was optimized, evaluating two chiral columns, Chirobiotic V and Chiralpal-AY3. Better resolution was obtained using a Chiralpak-AY3 column, utilizing as mobile phase ethanol (0.1% diethanolamine). An extraction procedure based on a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) was applied, using water and acetonitrile containing 1% of acetic acid, and a clean-up step utilizing primary secondary amine (PSA) and graphitized carbon black (GCB) as sorbents. The extract was diluted with ethanol (50/:50, v/v) prior to chromatographic analysis, and the separation was carried out avoiding the racemization during this stage. Enantiomerization process of atropine was studied in samples at different conditions such as temperature (30, 50 and 80°C) and pH (3, 5, 7 and 9), observing that racemization occurs at high pH (9) and temperature (80°C). Stramonium and Brugmansia seeds were analyzed and the concentration of (-)-hyoscyamine was 1500mg/kg and 320mg/kg respectively. Contaminated buckwheat was also determined and (-)-hyoscyamine was detected at 170µg/kg.
[Mh] Termos MeSH primário: Atropina/química
Solanaceae/química
[Mh] Termos MeSH secundário: Atropina/isolamento & purificação
Cromatografia Líquida de Alta Pressão
Datura stramonium/química
Fagopyrum/química
Concentração de Íons de Hidrogênio
Hiosciamina/análise
Indicadores e Reagentes
Limite de Detecção
Reprodutibilidade dos Testes
Sementes/química
Solventes
Estereoisomerismo
Espectrometria de Massas em Tandem
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Indicators and Reagents); 0 (Solvents); 7C0697DR9I (Atropine); PX44XO846X (Hyoscyamine)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171201
[Lr] Data última revisão:
171201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


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[PMID]:28873551
[Au] Autor:Cirlini M; Demuth TM; Biancardi A; Rychlik M; Dall'Asta C; Bruni R
[Ad] Endereço:Department of Food and Drug - LS9 Interlab Group, University of Parma, Via G.P. Usberti 95/a, 43134 Parma, Italy.
[Ti] Título:Are tropane alkaloids present in organic foods? Detection of scopolamine and atropine in organic buckwheat (Fagopyron esculentum L.) products by UHPLC-MS/MS.
[So] Source:Food Chem;239:141-147, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A closer monitoring of tropane alkaloids (TA) in foods is now recommended by the European Commission, following a series of alerts related to the contamination of buckwheat with weeds of the genus Datura. A novel, accurate UHPLC-MS/MS method was developed and validated for the rapid detection of scopolamine and atropine in buckwheat foods. A suitable extraction protocol was set up to maximize recoveries and detection limits in different raw, processed and baked foods. The method offers good performances in terms of sensitivity, accuracy and precision, with LOQs at 0.04 and 0.10µg/kg. The established method is suitable for routine determination of trace levels of TA and was applied to 26 different buckwheat-derived organic foods, detecting TA in 3 samples (13.9-83.9µg/kg for atropine and 5.7-10.4µg/kg for scopolamine). Only in one case the level of contamination was relevant in terms of food safety.
[Mh] Termos MeSH primário: Alimentos Orgânicos
[Mh] Termos MeSH secundário: Alcaloides
Atropina
Cromatografia Líquida de Alta Pressão
Fagopyrum
Seres Humanos
Hidrobrometo de Escopolamina
Espectrometria de Massas em Tandem
Tropanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Tropanes); 451IFR0GXB (Scopolamine Hydrobromide); 7C0697DR9I (Atropine)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


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[PMID]:28799853
[Au] Autor:Xue F; Wu Z; Tong J; Zheng J; Li C
[Ad] Endereço:a School of Pharmacy , Nanjing University of Chinese Medicine , Nanjing , China.
[Ti] Título:Effect of combination of high-intensity ultrasound treatment and dextran glycosylation on structural and interfacial properties of buckwheat protein isolates.
[So] Source:Biosci Biotechnol Biochem;81(10):1891-1898, 2017 Oct.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study investigated the effects of high-intensity ultrasound and glycosylation on the structural and interfacial properties of the Maillard reaction conjugates of buckwheat protein isolate (BPI). The covalent attachment of dextran to BPI was confirmed by examination of the Fourier-transform infrared spectra. Emulsifying properties of the conjugates obtained by ultrasound treatment were improved as compared to those obtained by classical heating. Structural feature analyses suggested that conjugates obtained by ultrasound treatment had less α-helix and more random coil, higher surface hydrophobicity and less compact tertiary structure as compared to those obtained by classical heating. The surface activity measurement revealed that the BPI-dextran conjugates obtained by ultrasound treatment were closely packed and that each molecule occupied a small area of the interface. Combination of ultrasonic treatment and glycosylation was proved to be an efficient way to develop new stabilizers and thickening agents for food in this study.
[Mh] Termos MeSH primário: Dextranos/metabolismo
Fagopyrum/química
Proteínas de Plantas/química
Proteínas de Plantas/metabolismo
Ondas Ultrassônicas
[Mh] Termos MeSH secundário: Adsorção
Glicosilação
Interações Hidrofóbicas e Hidrofílicas
Reação de Maillard
Óleos/química
Estabilidade Proteica
Propriedades de Superfície
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Dextrans); 0 (Oils); 0 (Plant Proteins); 059QF0KO0R (Water)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170812
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1361805


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[PMID]:28764034
[Au] Autor:Bai YP; Guo XN; Zhu KX; Zhou HM
[Ad] Endereço:State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, PR China; School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu Province, PR China.
[Ti] Título:Shelf-life extension of semi-dried buckwheat noodles by the combination of aqueous ozone treatment and modified atmosphere packaging.
[So] Source:Food Chem;237:553-560, 2017 Dec 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The present study investigated the combined effects of aqueous ozone treatment and modified atmosphere packaging (MAP) on prolonging the shelf-life of semi-dried buckwheat noodles [SBWN; moisture content (22.5±0.5%)] at 25°C. Firstly, the different concentrations of ozonated water were used to make SBWN. Subsequently, SBWN prepared with ozonated water were packaged under six different conditions and stored for 11days. Changes in microbial, chemical-physical, textural properties and sensorial qualities of SWBN were monitored during storage. Microbiological results indicated that adopting 2.21mg/L of ozonated water resulted in a 1.8 log CFU/g reduction of the initial microbial loads in SBWN. In addition, MAP suppressed the microbial growth with a concomitant reduction in the rates of acidification and quality deteriorations of SBWN. Finally, the shelf-life of sample packed under N :CO =30:70 was extended to 9days, meanwhile textural and sensorial characteristics were maintained during the whole storage period.
[Mh] Termos MeSH primário: Fagopyrum
Embalagem de Alimentos
[Mh] Termos MeSH secundário: Atmosfera
Microbiologia de Alimentos
Conservação de Alimentos
Expectativa de Vida
Nitrogênio
Ozônio
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
66H7ZZK23N (Ozone); N762921K75 (Nitrogen)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171107
[Lr] Data última revisão:
171107
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170803
[St] Status:MEDLINE


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[PMID]:28582812
[Au] Autor:Dinu M; Macchia D; Pagliai G; Gori AM; Cesari F; Marcucci R; Sofi F; Casini A
[Ad] Endereço:Department of Experimental and Clinical Medicine, School of Human Health Sciences, University of Florence, Italy. Email: mdinu@unifi.it.
[Ti] Título:Symptomatic efficacy of buckwheat products in Non-Celiac Gluten Sensitivity (NCGS).
[So] Source:Asia Pac J Clin Nutr;26(4):630-636, 2017.
[Is] ISSN:0964-7058
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:BACKGROUND AND OBJECTIVES: Buckwheat (Fagopyrum esculentum) is a gluten-free grain with acclaimed benefi-cial effects on human health. Our aim was to assess the effect of buckwheat products on intestinal/extra-intestinal symptoms and biochemical parameters in patients with Non-Celiac Gluten Sensitivity (NCGS). METHODS AND STUDY DESIGN: A randomized, crossover trial with two intervention phases was conducted on 19 NCGS patients over a 12 week-period. The participants were assigned to consume products made from buckwheat or to maintain their normal gluten-free diet for 6 weeks in a random order. Symptoms due to NCGS were evaluated using two questionnaires. RESULTS: During the intervention period with buckwheat products, patients experienced a signifi-cant decrease in the severity of abdominal pain and bloating (p=0.03). In contrast, the control group showed a significant worsening trend for the majority of NCGS symptoms such as nausea, headache, joint/muscle pain, and attention disorders. The replacement diet with buckwheat products also resulted in a significant increase of serum magnesium (+4.7%) and a significant reduction in the circulating levels of some pro-inflammatory cytokines such as interferon gamma (-33.3%) and monocyte chemotactic protein-1 (-46.5%). CONCLUSION: The study supports the positive effects of buckwheat for NCGS patients, showing that this alternative cereal can contribute to the re-duction of both negative gastro-intestinal and related symptoms, and nutritional deficiencies, and lead to an im-provement in inflammatory profile.
[Mh] Termos MeSH primário: Doença Celíaca
Fagopyrum
Análise de Alimentos
[Mh] Termos MeSH secundário: Adulto
Idoso
Feminino
Seres Humanos
Masculino
Meia-Idade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170831
[Lr] Data última revisão:
170831
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170606
[St] Status:MEDLINE
[do] DOI:10.6133/apjcn.072016.07


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[PMID]:28513535
[Au] Autor:Wen YQ; Zhang J; Li Y; Chen L; Zhao W; Zhou J; Jin Y
[Ad] Endereço:Institute of Apicultural Research, Chinese Academy of Agricultural Sciences, Beijing 100093, China. wenyaqin1219@163.com.
[Ti] Título:Characterization of Chinese Unifloral Honeys Based on Proline and Phenolic Content as Markers of Botanical Origin, Using Multivariate Analysis.
[So] Source:Molecules;22(5), 2017 May 17.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The phenolic and proline content were determined in honey samples of different floral origins (rapeseed, sunflower, buckwheat and ) from five different regions of China. The phenolic and proline profile of these samples were used to construct a statistical model to distinguish honeys from different floral origins. Significant differences were identified among the studied honey samples from multivariate chemometric methods. The proline content varied among the four types of honeys, with the values decreasing in the order: buckwheat > > sunflower > rapeseed. Rapeseed honeys contained a high level of benzoic acid, while rutin, -coumaric acid, -hydroxybenzoic acid were present at relatively high levels in buckwheat honeys. Principal component analysis (PCA) revealed that rapeseed honey could be distinguished from the other three unifloral honeys, and benzoic acid, proline and kaempferol could serve as potential floral markers. Using 18 phenolic compounds and proline the honey samples were satisfactorily classified according to floral origin at 94% correct prediction by linear discriminant analysis (LDA). The results indicated that phenolic compounds and proline were useful for the identification of the floral origin of the four type honeys.
[Mh] Termos MeSH primário: Flores
Mel/análise
Mel/classificação
Fenóis/análise
Prolina/análise
[Mh] Termos MeSH secundário: Brassica rapa
China
Cromatografia Líquida de Alta Pressão
Codonopsis
Fagopyrum
Helianthus
Análise Multivariada
Análise de Componente Principal
Extração em Fase Sólida
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phenols); 9DLQ4CIU6V (Proline)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170524
[Lr] Data última revisão:
170524
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170518
[St] Status:MEDLINE


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[PMID]:28489364
[Au] Autor:Amézqueta S; Ramos-Romero S; Martínez-Guimet C; Moreno A; Hereu M; Torres JL
[Ad] Endereço:Departament d'Enginyeria Química i Química Analítica and Institut de Biomedicina (IBUB), Universitat de Barcelona , Martí i Franquès 1-11, 08028 Barcelona, Spain.
[Ti] Título:Fate of d-Fagomine after Oral Administration to Rats.
[So] Source:J Agric Food Chem;65(22):4414-4420, 2017 Jun 07.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:d-Fagomine is an iminosugar found in buckwheat that is capable of inhibiting the adhesion of potentially pathogenic bacteria to epithelial mucosa and reducing the postprandial blood glucose concentration. This paper evaluates the excretion and metabolism of orally administered d-fagomine in rats and compares outcomes with the fate of 1-deoxynojirimycin. d-Fagomine and 1-deoxynojirimycin show similar absorption and excretion kinetics. d-Fagomine is partly absorbed (41-84%, dose of 2 mg/kg of body weight) and excreted in urine within 8 h, while the non-absorbed fraction is cleared in feces within 24 h. d-Fagomine is partially methylated (about 10% in urine and 3% in feces). The concentration of d-fagomine in urine from 1 to 6 h after administration is higher than 10 mg/L, the concentration that inhibits adhesion of Escherichia coli. Orally administered d-fagomine is partially absorbed and then rapidly excreted in urine, where it reaches a concentration that may be protective against urinary tract infections.
[Mh] Termos MeSH primário: Fagopyrum/química
Imino Piranoses/farmacocinética
Extratos Vegetais/farmacocinética
[Mh] Termos MeSH secundário: Administração Oral
Animais
Cromatografia Líquida de Alta Pressão
Imino Piranoses/administração & dosagem
Imino Piranoses/urina
Masculino
Espectrometria de Massas
Extratos Vegetais/administração & dosagem
Extratos Vegetais/urina
Ratos
Ratos Sprague-Dawley
Distribuição Tecidual
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Imino Pyranoses); 0 (Plant Extracts); 53185-12-9 (fagomine)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170619
[Lr] Data última revisão:
170619
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170511
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01026


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[PMID]:28400025
[Au] Autor:Ua-Arak T; Jakob F; Vogel RF
[Ad] Endereço:Technische Universität München, Lehrstuhl für Technische Mikrobiologie, Gregor-Mendel-Straße 4, 85354 Freising, Germany.
[Ti] Título:Influence of levan-producing acetic acid bacteria on buckwheat-sourdough breads.
[So] Source:Food Microbiol;65:95-104, 2017 Aug.
[Is] ISSN:1095-9998
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Buckwheat sourdoughs supplemented with molasses as natural sucrose source were fermented with levan-producing Gluconobacter (G.) albidus TMW 2.1191 and Kozakia (K.) baliensis NBRC 16680. Cell growth, concomitant levan and low-molecular-weight metabolite production were monitored. Sourdough breads were prepared with different sourdoughs from both strains (24, 30 and 48 h fermentation, respectively) and analyzed with respect to bread volume, crumb hardness and sensory characteristics. During fermentation, levan, acetic and gluconic acids were increasingly produced, while spontaneously co-growing lactic acid bacteria additionally formed acetic and lactic acids. Sourdoughs from both strains obtained upon 24 h of fermentation significantly improved the bread sensory and quality, including higher specific volume as well as lower crumb hardness. Buckwheat doughs containing isolated levan, with similar molecular size and mass compared to in situ produced levan in the sourdough at 48 h, verified the positive effect of levan on bread quality. However, the positive effects of levan were masked to a certain extent by the impact from the natural acidification during fermentations. While levan-producing acetic acid bacteria are a promising alternative for the development of clean-label gluten-free breads without the need of additives, an appropriate balance between acidification and levan production (amount and structure) must be reached.
[Mh] Termos MeSH primário: Ácido Acético/metabolismo
Acetobacteraceae/metabolismo
Pão/microbiologia
Fagopyrum/microbiologia
Frutanos/biossíntese
Gluconobacter/metabolismo
[Mh] Termos MeSH secundário: Acetobacteraceae/crescimento & desenvolvimento
Antineoplásicos
Bactérias/metabolismo
Pão/análise
Fermentação
Farinha/microbiologia
Microbiologia de Alimentos
Frutanos/metabolismo
Gluconobacter/crescimento & desenvolvimento
Glutens
Lactobacillaceae/crescimento & desenvolvimento
Lactobacillaceae/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Fructans); 8002-80-0 (Glutens); 9013-95-0 (levan); Q40Q9N063P (Acetic Acid)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170427
[Lr] Data última revisão:
170427
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170413
[St] Status:MEDLINE



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