Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.825.359 [Categoria DeCS]
Referências encontradas : 5 [refinar]
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  1 / 5 MEDLINE  
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[PMID]:27575805
[Au] Autor:Bzdega K; Janiak A; Ksiazczyk T; Lewandowska A; Gancarek M; Sliwinska E; Tokarska-Guzik B
[Ad] Endereço:Department of Botany and Nature Protection, University of Silesia, Katowice, Poland.
[Ti] Título:A Survey of Genetic Variation and Genome Evolution within the Invasive Fallopia Complex.
[So] Source:PLoS One;11(8):e0161854, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The knotweed taxa Fallopia japonica, F. sachalinensis and their interspecific hybrid F. × bohemica are some of the most aggressive invaders in Europe and North America and they are serious threats to native biodiversity. At the same time, they constitute a unique model system for the creation of hybrids and studies of the initiation of evolutionary processes. In the presented study, we focused on (i) examining genetic diversity in selected populations of three Fallopia taxa in the invaded (Poland) and native ranges (Japan), (ii) establishing genome size and ploidy levels and (iii) identifying ribosomal DNA (rDNA)-bearing chromosomes in all of the taxa from the invaded range. We found that the genetic diversity within particular taxa was generally low regardless of their geographical origin. A higher level of clonality was observed for the Polish populations compared to the Japanese populations. Our study suggests that the co-occurrence of F. sachalinensis together with the other two taxa in the same stand may be the source of the higher genetic variation within the F. × bohemica hybrid. Some shift towards the contribution of F. japonica alleles was also observed for selected F. × bohemica individuals, which indicates the possibility of producing more advanced generations of F. × bohemica hybrids. All of the F. sachalinensis individuals were hexaploid (2n = 6x = 66; 2C = 6.01 pg), while those of F. japonica were mostly octoploid (2n = 8x = 88; 2C = 8.87 pg) and all of the F. × bohemica plants except one were hexaploid (2n = 6x = 66; 2C = 6.46 pg). Within the chromosome complement of F. japonica, F. sachalinensis and F. × bohemica, the physical mapping of the rDNA loci provided markers for 16, 13 and 10 chromosomes, respectively. In F. × bohemica, a loss of some of rDNA loci was observed, which indicates the occurrence of genome changes in the hybrid.
[Mh] Termos MeSH primário: DNA Ribossômico/genética
Fallopia/genética
Variação Genética
[Mh] Termos MeSH secundário: Evolução Molecular
Tamanho do Genoma
Genoma de Planta
Espécies Introduzidas
Japão
Filogenia
Filogeografia
Mapeamento Físico do Cromossomo
Polônia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Ribosomal)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160831
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0161854


  2 / 5 MEDLINE  
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[PMID]:27032213
[Au] Autor:Békési-Kallenberger H; Horváth G; Bencsik T; Balázs VL; Filep R; Papp N
[Ti] Título:Comparative Histological and Phytochemical Study of Fallopia species.
[So] Source:Nat Prod Commun;11(2):251-4, 2016 Feb.
[Is] ISSN:1934-578X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fallopia species which belong to the Polygonaceae family have several data related to their use in the Asian herbal medicine. In this work, some histological and phytochemical parameters of Fallopia japonica, F. sachalinensis, and F. x bohemica were analysed and compared. Rhizome and leaf samples were collected before, during, and after the flowering period at 3 habitats in Szombathely and 4 habitats in Baranya County, Hungary. The main histological characteristics of the stem, leaf and petiole were studied by light microscopy in cross section. Total tannin and anthraquinone contents were determined according to the official methods of the Hungarian Pharmacopoeia VIIIth (equal to the European Pharmacopoeia 6th). No species-specific markers were found in any plant part. In the rhizome, the highest tannin content was measured in Japanese knotweed, followed by Bohemian and giant knotweed in each period. The tannin content measured in each plant was higher in the leaves than in the rhizomes except F. japonica. The rhizome of F. japonica had the highest anthraquinone content before the flowering period, followed by F. x bohemica and F. sachalinensis. According to earlier and our preliminary data, Fallopia taxa are of great therapeutic promise in the future.
[Mh] Termos MeSH primário: Fallopia/química
Fallopia/classificação
Compostos Fitoquímicos/química
[Mh] Termos MeSH secundário: Folhas de Planta/química
Caules de Planta/química
Especificidade da Espécie
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phytochemicals)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160401
[Lr] Data última revisão:
160401
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160402
[St] Status:MEDLINE


  3 / 5 MEDLINE  
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[PMID]:26928800
[Au] Autor:Yamaguchi T; Noge K; Asano Y
[Ad] Endereço:Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, 5180 Kurokawa, Imizu, Toyama, 939-0398, Japan.
[Ti] Título:Cytochrome P450 CYP71AT96 catalyses the final step of herbivore-induced phenylacetonitrile biosynthesis in the giant knotweed, Fallopia sachalinensis.
[So] Source:Plant Mol Biol;91(3):229-39, 2016 Jun.
[Is] ISSN:1573-5028
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:The giant knotweed Fallopia sachalinensis (Polygonaceae) synthesizes phenylacetonitrile (PAN) from L-phenylalanine when infested by the Japanese beetle Popillia japonica or treated with methyl jasmonate (MeJA). Here we identified (E/Z)-phenylacetaldoxime (PAOx) as the biosynthetic precursor of PAN and identified a cytochrome P450 that catalysed the conversion of (E/Z)-PAOx to PAN. Incorporation of deuterium-labelled (E/Z)-PAOx into PAN emitted from the leaves of F. sachalinensis was detected using gas chromatography-mass spectrometry. Further, using liquid chromatography-tandem mass spectrometry, we detected the accumulation of (E/Z)-PAOx in MeJA-treated leaves. These results showed that (E/Z)-PAOx is the biosynthetic precursor of PAN. MeJA-induced mRNAs were analysed by differential expression analysis using a next-generation sequencer. Of the 74,329 contigs obtained from RNA-seq and de novo assembly, 252 contigs were induced by MeJA treatment. Full-length cDNAs encoding MeJA-induced cytochrome P450s CYP71AT96, CYP82AN1, CYP82D125 and CYP715A35 were cloned using 5'- and 3'-RACE and were expressed using a baculovirus expression system. Among these cytochrome P450s, CYP71AT96 catalysed the conversion of (E/Z)-PAOx to PAN in the presence of NADPH and a cytochrome P450 reductase. It also acted on (E/Z)-4-hydroxyphenylacetaldoxime and (E/Z)-indole-3-acetaldoxime. The broad substrate specificity of CYP71AT96 was similar to that of aldoxime metabolizing cytochrome P450s. Quantitative RT-PCR analysis showed that CYP71AT96 expression was highly induced because of treatment with MeJA as well as feeding by the Japanese beetle. These results indicate that CYP71AT96 likely contributes the herbivore-induced PAN biosynthesis in F. sachalinensis.
[Mh] Termos MeSH primário: Acetonitrilos/metabolismo
Sistema Enzimático do Citocromo P-450/metabolismo
Fallopia/metabolismo
[Mh] Termos MeSH secundário: Acetatos/farmacologia
Animais
Catálise
Coleópteros
Ciclopentanos/farmacologia
Sistema Enzimático do Citocromo P-450/genética
Fallopia/efeitos dos fármacos
Fallopia/fisiologia
Cromatografia Gasosa-Espectrometria de Massas
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Regulação da Expressão Gênica de Plantas/fisiologia
Herbivoria
Oxilipinas/farmacologia
Filogenia
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Acetonitriles); 0 (Cyclopentanes); 0 (Oxylipins); 0 (Plant Proteins); 23G40PRP93 (benzyl cyanide); 900N171A0F (methyl jasmonate); 9035-51-2 (Cytochrome P-450 Enzyme System)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160302
[St] Status:MEDLINE
[do] DOI:10.1007/s11103-016-0459-6


  4 / 5 MEDLINE  
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[PMID]:26906096
[Au] Autor:Bardon C; Poly F; Piola F; Pancton M; Comte G; Meiffren G; Haichar Fel Z
[Ad] Endereço:Université de Lyon, UMR 5557 LEM, Université Lyon 1, CNRS, INRA USC 1364, F-69622 Villeurbanne Cedex, France Université de Lyon, UMR5023 LEHNA, Université Lyon 1, CNRS, ENTPE, F-69622 Villeurbanne Cedex, France clementbardon@hotmail.fr.
[Ti] Título:Mechanism of biological denitrification inhibition: procyanidins induce an allosteric transition of the membrane-bound nitrate reductase through membrane alteration.
[So] Source:FEMS Microbiol Ecol;92(5):fiw034, 2016 May.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Recently, it has been shown that procyanidins from Fallopia spp. inhibit bacterial denitrification, a phenomenon called biological denitrification inhibition (BDI). However, the mechanisms involved in such a process remain unknown. Here, we investigate the mechanisms of BDI involving procyanidins, using the model strain Pseudomonas brassicacearum NFM 421. The aerobic and anaerobic (denitrification) respiration, cell permeability and cell viability of P. brassicacearum were determined as a function of procyanidin concentration. The effect of procyanidins on the bacterial membrane was observed using transmission electronic microscopy. Bacterial growth, denitrification, NO3- and NO2-reductase activity, and the expression of subunits of NO3- (encoded by the gene narG) and NO2-reductase (encoded by the gene nirS) under NO3 or NO2 were measured with and without procyanidins. Procyanidins inhibited the denitrification process without affecting aerobic respiration at low concentrations. Procyanidins also disturbed cell membranes without affecting cell viability. They specifically inhibited NO3- but not NO2-reductase.Pseudomonas brassicacearum responded to procyanidins by over-expression of the membrane-bound NO3-reductase subunit (encoded by the gene narG). Our results suggest that procyanidins can specifically inhibit membrane-bound NO3-reductase inducing enzymatic conformational changes through membrane disturbance and that P. brassicacearum responds by over-expressing membrane-bound NO3-reductase. Our results lead the way to a better understanding of BDI.
[Mh] Termos MeSH primário: Desnitrificação
Fallopia/metabolismo
Fallopia/microbiologia
Nitrato Redutase/metabolismo
Proantocianidinas/metabolismo
Pseudomonas/enzimologia
[Mh] Termos MeSH secundário: Regulação Alostérica
Biflavonoides
Catequina
Membrana Celular/metabolismo
Membrana Celular/ultraestrutura
Nitrato Redutase/química
Nitratos/metabolismo
Oxirredução
Oxirredutases/genética
Pseudomonas/metabolismo
Pseudomonas/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Biflavonoids); 0 (Nitrates); 0 (Proanthocyanidins); 4852-22-6 (procyanidin); 8R1V1STN48 (Catechin); EC 1.- (Oxidoreductases); EC 1.7.99.4 (Nitrate Reductase)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160225
[St] Status:MEDLINE


  5 / 5 MEDLINE  
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[PMID]:26411284
[Au] Autor:Bardon C; Piola F; Haichar Fel Z; Meiffren G; Comte G; Missery B; Balby M; Poly F
[Ad] Endereço:Université de Lyon, UMR 5557 LEM, Université Lyon 1, CNRS, INRA USC 1364, F-69622, Villeurbanne Cedex, France.
[Ti] Título:Identification of B-type procyanidins in Fallopia spp. involved in biological denitrification inhibition.
[So] Source:Environ Microbiol;18(2):644-55, 2016 Feb.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Nitrogen (N) is considered as a main limiting factor in plant growth, and nitrogen losses through denitrification can be responsible for severe decreases in plant productivity. Recently, it was demonstrated that Fallopia spp. is responsible for biological denitrification inhibition (BDI) through the release of unknown secondary metabolites. Here, we investigate the secondary metabolites involved in the BDI of Fallopia spp. The antioxidant, protein precipitation capability of Fallopia spp. extracts was measured in relation to the aerobic respiration and denitrification of two bacteria (Gram positive and Gram negative). Proanthocyanidin concentrations were estimated. Proanthocyanidins in extracts were characterized by chromatographic analysis, purified and tested on the bacterial denitrification and aerobic respiration of two bacterial strains. The effect of commercial procyanidins on denitrification was tested on two different soil types. Denitrification and aerobic respiration inhibition were correlated with protein precipitation capacity and concentration of proanthocyanidins but not to antioxidant capacity. These proanthocyanidins were B-type procyanidins that inhibited denitrification more than the aerobic respiration of bacteria. In addition, procyanidins also inhibited soil microbial denitrification. We demonstrate that procyanidins are involved in the BDI of Fallopia spp. Our results pave the way to a better understanding of plant-microbe interactions and highlight future applications for a more sustainable agriculture.
[Mh] Termos MeSH primário: Biflavonoides/metabolismo
Catequina/metabolismo
Desnitrificação/fisiologia
Fallopia/metabolismo
Nitrogênio/metabolismo
Proantocianidinas/metabolismo
[Mh] Termos MeSH secundário: Agricultura
Antioxidantes/fisiologia
Biflavonoides/farmacologia
Catequina/farmacologia
Fallopia/genética
Proantocianidinas/farmacologia
Solo/química
Microbiologia do Solo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biflavonoids); 0 (Proanthocyanidins); 0 (Soil); 15514-06-4 (procyanidin B); 8R1V1STN48 (Catechin); N762921K75 (Nitrogen)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150929
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13062



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