Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.859.625.750 [Categoria DeCS]
Referências encontradas : 33 [refinar]
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[PMID]:28573218
[Au] Autor:Sangkaruk R; Rungrojsakul M; Tima S; Anuchapreeda S
[Ad] Endereço:Division of Clinical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200 Thailand.
[Ti] Título:EFFECT OF THAI SARAPHI FLOWER EXTRACTS ON WT1 AND BCR/ABL PROTEIN EXPRESSION IN LEUKEMIC CELL LINES.
[So] Source:Afr J Tradit Complement Altern Med;14(2):16-24, 2017.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Saraphi is a Thai traditional herb. In this study, the cytotoxic effects of crude ethanolic and fractional extracts including hexane, ethyl acetate, and methanol fractions from flowers were investigated in order to determine their effect on WT1 expression in Molt4 and K562 cells and Bcr/Abl expression in K562 cells. MATERIALS AND METHODS: The flowers of were extracted using ethanol. The ethanol flower extract was further fractionated with hexane, ethyl acetate, and methanol. Cytotoxic effects were measured by the MTT assay. Bcr/Abl and WT1 protein levels after treatments were determined by Western blotting. The total cell number was determined the typan blue exclusion method. RESULTS: The hexane fraction showed the strongest cytotoxic activity on Molt4 and K562 cells, with IC values of 2.6 and 77.6 µg/ml, respectively. The hexane extract decreased Bcr/Abl protein expression in K562 cells by 74.6% and WT1 protein expressions in Molt4 and K562 cells by 68.4 and 72.1%, respectively. Total cell numbers were decreased by 66.2 and 48.7% in Molt4 and K562 cells, respectively. Mammea E/BB (main active compound) significantly decreased both Bcr/Abl and WTlprotein expressions by 75 and 49.5%, respectively when compared to vehicle control. CONCLUSION: The hexane fraction from flowers inhibited cell proliferation the suppression of WT1 expression in Molt4 and K562 cells and Bcr/Abl expression in K562 cells. The active compound may be mammea E/BB. Extracts from flowers show promise as naturally occurring anti-cancer drugs.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/uso terapêutico
Proteínas de Fusão bcr-abl/metabolismo
Leucemia/tratamento farmacológico
Mammea
Fitoterapia
Extratos Vegetais/uso terapêutico
Proteínas WT1/metabolismo
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/farmacologia
Proliferação Celular
Flores
Seres Humanos
Células K562
Leucemia/metabolismo
Medicina Tradicional
Proteínas Oncogênicas v-abl/metabolismo
Extratos Vegetais/farmacologia
Proteínas Proto-Oncogênicas c-bcr/metabolismo
Tailândia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Oncogene Proteins v-abl); 0 (Plant Extracts); 0 (WT1 Proteins); 0 (WT1 protein, human); 0 (abl-bcr fusion protein, human); EC 2.7.10.2 (Fusion Proteins, bcr-abl); EC 2.7.11.1 (BCR protein, human); EC 2.7.11.1 (Proto-Oncogene Proteins c-bcr)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170816
[Lr] Data última revisão:
170816
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v14i2.3


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[PMID]:28551236
[Au] Autor:Péroumal A; Adenet S; Rochefort K; Fahrasmane L; Aurore G
[Ad] Endereço:Université des Antilles, UMR 1270 QUALITROP, F-97 110 Pointe-à-Pitre, France. Electronic address: armelle_peroumal@yahoo.fr.
[Ti] Título:Variability of traits and bioactive compounds in the fruit and pulp of six mamey apple (Mammea americana L.) accessions.
[So] Source:Food Chem;234:269-275, 2017 Nov 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Our objective was to compare fruit morphology, physico-chemistry and bioactive compounds content of the edible pulp of six Mammea americana accessions. The results showed that this fruit was rather big, weighing on average 600 to 1100g depending on the accession, and spherical to oblate-shaped. The pulp represented between 50 and 70% of the weight of the whole fruit. The pulp adhered only partially to the seeds in 5 of the 6 accessions studied, while the last one exhibited full adherence. The fresh pulp was acidic, sweet, succulent and crunchy. The fruits studied had a variety of qualities, providing various opportunities for post-harvest uses: fruit salads, nectar preparation, jams and jellies, or export. We have established for the first time the total phenolic compounds and total flavonoids contents in the pulp of mamey apple fruits. The pulp colour was highly correlated with total phenolic compounds and total carotenoids contents.
[Mh] Termos MeSH primário: Carotenoides/química
Flavonoides/química
Frutas/química
Mammea/química
Fenóis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonoids); 0 (Phenols); 36-88-4 (Carotenoids)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170529
[St] Status:MEDLINE


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[PMID]:28504908
[Au] Autor:Li C; Jeong Y; Kim M
[Ad] Endereço:1 College of Tourism and Culinary Science, Yangzhou University , Yangzhou, China .
[Ti] Título:Mammea longifolia Planch. and Triana Fruit Extract Induces Cell Death in the Human Colon Cancer Cell Line, SW480, via Mitochondria-Related Apoptosis and Activation of p53.
[So] Source:J Med Food;20(5):485-490, 2017 May.
[Is] ISSN:1557-7600
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The methanol extract of Mammea longifolia Planch. and Triana (M. longifolia) fruit was studied for anticancer and apoptotic effects in the SW480 colon cancer cell line. The apoptotic and necrotic effects of M. longifolia were detected by 3-(4,5-dimethyl-2-thiazolyl)-2, 5-diphenyl-2H tetrazolium bromide (MTT) and lactate dehydrogenase assays, respectively. One hundred µg/mL of the extract killed ∼82.4% of the cells; however, 2% of the death was related to necrosis. The morphological changes in M. longifolia-stimulated SW480 cells were observed directly by light microscopy. DNA fragmentation assay was employed to analyze the apoptosis induction. M. longifolia-treated SW480 cells promoted the expression of Bax, Bad, cleaved-poly-ADP-ribose polymerase (PARP), and p53 proteins and decreased the protein expression of pro-caspases Bcl-2 and Bcl-XL. The ratios of Bax/Bcl-2 and cleaved-PARP/PARP, predictive markers of apoptotic stimuli in cancer, increased and may play an important role in regulating the progression of apoptosis. The results suggested that M. longifolia induces cell death via mitochondrial-related apoptosis in SW480 cells.
[Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos
Neoplasias do Colo/fisiopatologia
Mammea/química
Mitocôndrias/efeitos dos fármacos
Extratos Vegetais/farmacologia
Proteína Supressora de Tumor p53/metabolismo
[Mh] Termos MeSH secundário: Linhagem Celular Tumoral
Neoplasias do Colo/genética
Neoplasias do Colo/metabolismo
Fragmentação do DNA/efeitos dos fármacos
Frutas/química
Seres Humanos
Mitocôndrias/genética
Mitocôndrias/metabolismo
Poli(ADP-Ribose) Polimerases/genética
Poli(ADP-Ribose) Polimerases/metabolismo
Proteínas Proto-Oncogênicas c-bcl-2/genética
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Proteína Supressora de Tumor p53/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (TP53 protein, human); 0 (Tumor Suppressor Protein p53); EC 2.4.2.30 (Poly(ADP-ribose) Polymerases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE
[do] DOI:10.1089/jmf.2016.3865


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[PMID]:28100218
[Au] Autor:Gómez-Calderón C; Mesa-Castro C; Robledo S; Gómez S; Bolivar-Avila S; Diaz-Castillo F; Martínez-Gutierrez M
[Ad] Endereço:Grupo de Investigación en Ciencias Animales-GRICA, Universidad Cooperativa de Colombia, Calle 30A # 33-51, Bucaramanga, Colombia.
[Ti] Título:Antiviral effect of compounds derived from the seeds of Mammea americana and Tabernaemontana cymosa on Dengue and Chikungunya virus infections.
[So] Source:BMC Complement Altern Med;17(1):57, 2017 Jan 18.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The transmission of Dengue virus (DENV) and Chikungunya virus (CHIKV) has increased worldwide, due in part to the lack of a specific antiviral treatment. For this reason, the search for compounds with antiviral potential, either as licensed drugs or in natural products, is a research priority. The objective of this study was to identify some of the compounds that are present in Mammea americana (M. americana) and Tabernaemontana cymosa (T. cymosa) plants and, subsequently, to evaluate their cytotoxicity in VERO cells and their potential antiviral effects on DENV and CHIKV infections in those same cells. METHODS: Dry ethanolic extracts of M. americana and T. cymosa seeds were subjected to open column chromatographic fractionation, leading to the identification of four compounds: two coumarins, derived from M. americana; and lupeol acetate and voacangine derived from T. cymosa.. The cytotoxicity of each compound was subsequently assessed by the MTT method (at concentrations from 400 to 6.25 µg/mL). Pre- and post-treatment antiviral assays were performed at non-toxic concentrations; the resulting DENV inhibition was evaluated by Real-Time PCR, and the CHIKV inhibition was tested by the plating method. The results were analyzed by means of statistical analysis. RESULTS: The compounds showed low toxicity at concentrations ≤ 200 µg/mL. The compounds coumarin A and coumarin B, which are derived from the M. americana plant, significantly inhibited infection with both viruses during the implementation of the two experimental strategies employed here (post-treatment with inhibition percentages greater than 50%, p < 0.01; and pre-treatment with percentages of inhibition greater than 40%, p < 0.01). However, the lupeol acetate and voacangine compounds, which were derived from the T. cymosa plant, only significantly inhibited the DENV infection during the post-treatment strategy (at inhibition percentages greater than 70%, p < 0.01). CONCLUSION: In vitro, the coumarins are capable of inhibiting infection by DENV and CHIKV (with inhibition percentages above 50% in different experimental strategies), which could indicate that these two compounds are potential antivirals for treating Dengue and Chikungunya fever. Additionally, lupeol acetate and voacangine efficiently inhibit infection with DENV, also turning them into promising antivirals for Dengue fever.
[Mh] Termos MeSH primário: Antivirais/uso terapêutico
Febre de Chikungunya/tratamento farmacológico
Dengue/tratamento farmacológico
Mammea/química
Extratos Vegetais/uso terapêutico
Tabernaemontana/química
[Mh] Termos MeSH secundário: Animais
Cercopithecus aethiops
Citotoxinas/toxicidade
Mammea/toxicidade
Extratos Vegetais/toxicidade
Tabernaemontana/toxicidade
Células Vero
Replicação Viral/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antiviral Agents); 0 (Cytotoxins); 0 (Plant Extracts)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170120
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1562-1


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[PMID]:27373643
[Au] Autor:Ninomiya K; Shibatani K; Sueyoshi M; Chaipech S; Pongpiriyadacha Y; Hayakawa T; Muraoka O; Morikawa T
[Ad] Endereço:Pharmaceutical Research and Technology Institute, Kindai University.
[Ti] Título:Aromatase Inhibitory Activity of Geranylated Coumarins, Mammeasins C and D, Isolated from the Flowers of Mammea siamensis.
[So] Source:Chem Pharm Bull (Tokyo);64(7):880-5, 2016.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:A methanol extract of the flowers of Mammea siamensis (Calophyllaceae) was found to inhibit enzymatic activity against aromatase (IC50=16.5 µg/mL). From the extract, two new geranylated coumarins, mammeasins C (1) and D (2), were isolated together with seven coumarins: 8-hydroxy-5-methyl-7-(3,7-dimethyl-octa-2,6-dienyl)-9-(2-methyl-1-oxobutyl)-4,5-dihydropyrano[4,3,2-de]chromen-2-one (9), 8-hydroxy-5-methyl-7-(3,7-dimethyl-octa-2,6-dienyl)-9-(3-methyl-1-oxobutyl)-4,5-dihydropyrano[4,3,2-de]chromen-2-one (10), mammeas A/AA (14), A/AB (15), A/AA cyclo D (18), E/BA (23), and E/BC cyclo D (25). The structures of 1 and 2 were elucidated on the basis of spectroscopic evidence. Among the isolates including 17 previously reported coumarins, 1 (IC50=2.7 µM), 2 (3.6 µM), and mammea B/AB cyclo D (21, 3.1 µM) showed relatively strong inhibitory activities comparable to the activity of the synthetic nonsteroidal aromatase inhibitor aminoglutethimide (2.0 µM).
[Mh] Termos MeSH primário: Inibidores da Aromatase/farmacologia
Aromatase/metabolismo
Cumarínicos/farmacologia
Flores/química
Mammea/química
[Mh] Termos MeSH secundário: Inibidores da Aromatase/química
Inibidores da Aromatase/isolamento & purificação
Cumarínicos/química
Cumarínicos/isolamento & purificação
Relação Dose-Resposta a Droga
Seres Humanos
Estrutura Molecular
Proteínas Recombinantes/metabolismo
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Aromatase Inhibitors); 0 (Coumarins); 0 (Recombinant Proteins); 0 (mammeasin C); 0 (mammeasin D); EC 1.14.14.1 (Aromatase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170126
[Lr] Data última revisão:
170126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160705
[St] Status:MEDLINE
[do] DOI:10.1248/cpb.c16-00218


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[PMID]:27193767
[Au] Autor:Rungrojsakul M; Katekunlaphan T; Saiai A; Ampasavate C; Okonogi S; Sweeney CA; Anuchapreeda S
[Ad] Endereço:Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai, 50200, Thailand.
[Ti] Título:Down-regulatory mechanism of mammea E/BB from Mammea siamensis seed extract on Wilms' Tumor 1 expression in K562 cells.
[So] Source:BMC Complement Altern Med;16:130, 2016 May 18.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Wilms' tumor 1 (WT1) is a biological marker for predicting leukemia progression. In this study, mammea E/BB, an active compound from Saraphi (Mammea siamensis) seed extract was examined for its effect on down-regulatory mechanism of WT1 gene expression, WT1 protein and mRNA stability, and cell proliferation in K562 cell line. METHODS: M. siamensis seeds were obtained from the region of Chiang Mai (North of Thailand). Mammea E/BB was extracted from seeds of M. siamensis. WT1 protein expression and stability were evaluated by Western blot analysis. WT1 mRNA stability was assessed by qRT-PCR. WT1-DNA binding and WT1 promoter activity were assayed by ChIP assay and luciferase-reporter assay, respectively. Cell cycle arrest was studied by flow cytometry. RESULTS: Treatment with mammea E/BB led to down-regulation of WT1 expression. The suppression of WT1 expression did not involve protein and mRNA degradation. Rather, WT1 protein was down-regulated through disruption of transcriptional auto-regulation of the WT1 gene. Mammea E/BB inhibited WT1-DNA binding at the WT1 promoter and decreased luciferase activity. It also disrupted c-Fos/AP-1 binding to the WT1 promoter via ERK1/2 signaling pathway and induced S phase cell cycle arrest in K562 cells. CONCLUSION: Mammea E/BB had pleotropic effects on kinase signaling pathways, resulting in inhibition of leukemia cell proliferation.
[Mh] Termos MeSH primário: Cumarínicos/farmacologia
Regulação para Baixo/efeitos dos fármacos
Regulação Leucêmica da Expressão Gênica/efeitos dos fármacos
Mammea/química
Extratos Vegetais/farmacologia
Proteínas WT1/biossíntese
[Mh] Termos MeSH secundário: Proliferação Celular/efeitos dos fármacos
Cumarínicos/química
Seres Humanos
Células K562
Estrutura Molecular
Estabilidade de RNA/efeitos dos fármacos
RNA Neoplásico
Proteínas WT1/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coumarins); 0 (Mammea E-BB compound); 0 (Plant Extracts); 0 (RNA, Neoplasm); 0 (WT1 Proteins)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170117
[Lr] Data última revisão:
170117
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160520
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-016-1107-z


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[PMID]:25738951
[Au] Autor:Rungrojsakul M; Saiai A; Ampasavate C; Anuchapreeda S; Okonogi S
[Ad] Endereço:a Department of Pharmaceutical Science , Faculty of Pharmacy, Chiang Mai University , Chiang Mai 50200 , Thailand.
[Ti] Título:Inhibitory effect of mammea E/BB from Mammea siamensis seed extract on Wilms' tumour 1 protein expression in a K562 leukaemic cell line.
[So] Source:Nat Prod Res;30(4):443-7, 2016.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Mammea siamensis is used in traditional Thai medicine. This study was designed to extract and isolate an active compound from the M. siamensis seeds and to investigate its activity on Wilms' tumour 1 (WT1) protein expression in K562 cells. WT1 is a transcription factor that stimulates cell proliferation. The ethanol saraphi seed (ESS) extract was fractionated using n-hexane, ethyl acetate, n-butanol and water to obtain n-hexane saraphi seed (HSS), ethyl acetate saraphi seed (EASS), n-butanol saraphi seed (BSS), and water saraphi seed (WSS) extracts, respectively. The ESS, HSS and EASS extracts had strong cytotoxic effects on K562 cells in the MTT assay. All three fractions decreased WT1 protein levels and decreased total cell numbers. The HSS extract decreased the WT1 protein levels in a time- and dose-dependent manner. HPLC and NMR analyses indicated that the active compound of HSS was mammea E/BB. M. siamensis seeds are thus identified as a promising source of bioactive compounds for potential inhibition of WT1 protein expression.
[Mh] Termos MeSH primário: Cumarínicos/farmacologia
Mammea/química
Extratos Vegetais/farmacologia
Proteínas WT1/metabolismo
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Regulação Leucêmica da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Células K562/efeitos dos fármacos
Estrutura Molecular
Sementes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coumarins); 0 (Mammea E-BB compound); 0 (Plant Extracts); 0 (WT1 Proteins); 0 (WT1 protein, human)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150305
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2015.1017491


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[PMID]:26404214
[Au] Autor:Dang BT; Rouger C; Litaudon M; Richomme P; Séraphin D; Derbré S
[Ad] Endereço:EA 921 SONAS, Université D'Angers, SFR QUASAV 4207, Campus du Végétal, 42, rue Georges Morel, Beaucouzé 49070, France. taihoa1@gmail.com.
[Ti] Título:Identification of Minor Benzoylated 4-Phenylcoumarins from a Mammea neurophylla Bark Extract.
[So] Source:Molecules;20(10):17735-46, 2015 Sep 25.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Through dereplication analysis, seven known Mammea coumarins were identified in a fraction obtained from Mammea neurophylla dichloromethane bark extract selected for its ability to prevent advanced glycation end-product (AGE) formation. Among them, a careful examination of the NMR dataset of pedilanthocoumarin B led to a structural revision. Inspection of LC-DAD-MS(n) chromatograms allowed us to predict the presence of four new compounds, which were further isolated. Using spectroscopic methods (¹H-, (13)C- and 2D-NMR, HRMS, UV), these compounds were identified as new benzoyl substituted 4-phenylcoumarins (iso-pedilanthocoumarin B and neurophyllol C) and 4-(1-acetoxypropyl)coumarins cyclo F (ochrocarpins H and I).
[Mh] Termos MeSH primário: Cumarínicos/química
Mammea/química
Casca de Planta/química
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Coumarins); 0 (Plant Extracts); 13O55K0UYE (4-phenylcoumarin)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150926
[St] Status:MEDLINE
[do] DOI:10.3390/molecules201017735


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[PMID]:25518307
[Au] Autor:Inprasit J; Ruangnoo S; Itharat A
[Ti] Título:In vitro cytotoxic activity of Sa-Tri-Lhung-Klod remedy and its herbal ingredients on ovarian and cervical carcinoma cell lines.
[So] Source:J Med Assoc Thai;97 Suppl 8:S149-55, 2014 Aug.
[Is] ISSN:0125-2208
[Cp] País de publicação:Thailand
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Sa-Tri-Lhung-Klod is a Thai traditional medicine remedy for postpartum in the lists of The National Drug List ofHerbal Medicine Products AD. It consists ofseventeen herbs and were obtained by maceration and used in the form of liquor for women's health care such as treatment ofamenorrhea, menopause and blood tonic. In addition, it also usedfor postpartum care for being anti-inflammation in postpartum and prevention of cancer in women. OBJECTIVE: To investigate cytotoxic activity ofSa-Tri-Lhung-Klod remedy extracts and its herbal ingredients against human ovarian carcinoma cell line (SKOV-3) and cervical adenocarcinoma (HeLa) cell line. MATERIAL AND METHOD: Sa-Tri-Lhung-Klod remedy and its plant ingredients were extracted by maceration in 95% ethanol and dried using evaporator. All extracts were testedfor cytotoxic activity by sulforhodamine B (SRB) assay. RESULTS: Ethanolic extract ofSa-Tri-Lhung-Klod remedy displayed cytotoxic activity against SKOV-3 and HeLa with IC50 values of 72.84±1.07 and 47.24±2.83 µg/ml, respectively. It was classified as "less-active" according to the NCI guideline. However, Caesalpinia sappan, Mammea siamensis and Curcuma comosa showed high cytotoxic activity against SKOV-3 with IC50 values of 9.55±1.38 13.45±0.82 and 14.21±1.30 µg/ml, respectively. The ethanolic extracts ofCaesalpiniasappan and Mammea siamensis also exhibited cytotoxic activity against HeLa with IC50 values of 6.30±0.06 and 7.72±0.11 µg/ml, respectively. CONCLUSION: These results support the use of Sa-Tri-Lhung-Klod remedy in Thai traditional medicine for preventing of ovarian cancer and cervical cancer Caesalpinia sappan, Curcuma comosa and Mammea siamensis were strikingly active against ovarian and cervical cancer cells. Their extracts have the potential for developing as new anti-cancer drugs for women health.
[Mh] Termos MeSH primário: Adenocarcinoma
Neoplasias Ovarianas
Extratos Vegetais/farmacologia
Neoplasias do Colo do Útero
[Mh] Termos MeSH secundário: Caesalpinia
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Curcuma
Ensaios de Seleção de Medicamentos Antitumorais
Feminino
Células HeLa
Seres Humanos
Técnicas In Vitro
Mammea
Medicina Tradicional
Plantas Medicinais
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Extracts)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:141218
[Lr] Data última revisão:
141218
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141219
[St] Status:MEDLINE


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[PMID]:24731922
[Au] Autor:Dang BT; Gény C; Blanchard P; Rouger C; Tonnerre P; Charreau B; Rakolomalala G; Randriamboavonjy JI; Loirand G; Pacaud P; Litaudon M; Richomme P; Séraphin D; Derbré S
[Ad] Endereço:Université d'Angers, SFR QUASAV, EA 921 SONAS, Angers, France.
[Ti] Título:Advanced glycation inhibition and protection against endothelial dysfunction induced by coumarins and procyanidins from Mammea neurophylla.
[So] Source:Fitoterapia;96:65-75, 2014 Jul.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Advanced glycation end-products (AGEs) are associated with many pathogenic disorders such as pathogenesis of diabetes or endothelial dysfunction leading to cardiovascular events. Therefore, the identification of new anti-AGE molecules or extracts aims at preventing such pathologies. Many Clusiaceae and Calophyllaceae species are used in traditional medicines to treat arterial hypertension as well as diabetes. Focusing on these plant families, an anti-AGE plant screening allowed us to select Mammea neurophylla for further phytochemical and biological studies. Indeed, both DCM and MeOH stem bark extracts demonstrated in vitro their ability to prevent inflammation in endothelial cells and to reduce vasoconstriction. A bioguided fractionation of these extracts allowed us to point out 4-phenyl- and 4-(1-acetoxypropyl)coumarins and procyanidins as potent inhibitors of AGE formation, potentially preventing endothelial dysfunction. The fractionation steps also led to the isolation of two new compounds, namely neurophyllols A and B from the DCM bark extract together with thirteen known mammea A and E coumarins (mammea A/AA, mammea A/AB, mammea A/BA, mammea A/BB, mammea A/AA cycloD, mammea A/AB cycloD, disparinol B, mammea A/AB cycloE, ochrocarpin A, mammea A/AA cycloF, mammea A/AB cycloF, mammea E/BA, mammea E/BB) as well as δ-tocotrienol, xanthones (1-hydroxy-7-methoxyxanthone, 2-hydroxyxanthone) and triterpenes (friedelin and betulinic acid). During this study, R,S-asperphenamate, previously described from fungal origin was also purified.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Biflavonoides/farmacologia
Catequina/farmacologia
Cumarínicos/farmacologia
Produtos Finais de Glicação Avançada/efeitos dos fármacos
Mammea/química
Extratos Vegetais/farmacologia
Proantocianidinas/farmacologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos Fitogênicos/química
Antineoplásicos Fitogênicos/isolamento & purificação
Biflavonoides/química
Biflavonoides/isolamento & purificação
Catequina/química
Catequina/isolamento & purificação
Sobrevivência Celular/efeitos dos fármacos
Cumarínicos/química
Cumarínicos/isolamento & purificação
Células Endoteliais
Frutas/química
Masculino
Estrutura Molecular
Casca de Planta/química
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Folhas de Planta/química
Proantocianidinas/química
Proantocianidinas/isolamento & purificação
Ratos
Ratos Wistar
Triterpenos/química
Triterpenos/isolamento & purificação
Triterpenos/farmacologia
Xantonas/química
Xantonas/isolamento & purificação
Xantonas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Biflavonoids); 0 (Coumarins); 0 (Glycation End Products, Advanced); 0 (Plant Extracts); 0 (Proanthocyanidins); 0 (Triterpenes); 0 (Xanthones); 13O55K0UYE (4-phenylcoumarin); 4852-22-6 (procyanidin); 4G6A18707N (betulinic acid); 8R1V1STN48 (Catechin); AK21264UAD (friedelin)
[Em] Mês de entrada:1504
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140416
[St] Status:MEDLINE



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