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[PMID]:28812908
[Au] Autor:Farakos SMS; Pouillot R; Johnson R; Spungen J; Son I; Anderson N; Davidson GR; Doren JMV
[Ad] Endereço:1 U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, College Park, Maryland 20740 (ORCID: http://orcid.org/0000-0002-6107-5212 [R.P.]); and.
[Ti] Título:A Quantitative Assessment of the Risk of Human Salmonellosis Arising from the Consumption of Pecans in the United States.
[So] Source:J Food Prot;80(9):1574-1591, 2017 Sep.
[Is] ISSN:1944-9097
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A quantitative risk assessment was conducted to assess the risk of human salmonellosis acquired from consumption of pecans in the United States. The model considered the potential for Salmonella survival, growth, and recontamination of pecans from the sheller to the consumer, including steps such as immersion in water, drying, conditioning, cracking, partitioning, and storage. Five theoretical microbial reduction treatment levels (1 to 5 log CFU) were modeled. Data from the 2010 to 2013 surveys by the National Pecan Shellers Association were used for initial prevalence and contamination levels. The impacts of atypical situations in the pecan production system were also evaluated. Higher initial contamination levels, recontamination during processing, and a delay in drying postconditioning were the modeled atypical situations. The baseline model predicted a mean risk of salmonellosis in the United States from consumption of in-shell and shelled pecans processed by cold conditioning with no microbial reduction treatment and no further home cooking as 1 case per 775,193 servings (95% confidence interval [CI]: 1 case per 1,915,709 to 178,253 servings). This predicted risk per serving was estimated as a mean of 529 cases of salmonellosis per year (95% CI: 213 to 2,295 cases). Hot conditioning for shelled pecans and microbial reduction treatment of both shelled and in-shell pecans had a significant impact on the predicted mean risk of illness. Assuming 77% of the shelled pecans sold at retail (i.e., 80% of the retail supply) received hot conditioning, the mean estimated salmonellosis cases per year from consumption of in-shell and shelled pecans uncooked at home was 203 (95% CI: 81 to 882 cases) if no additional microbial reduction treatment were applied. The predicted risk of illness per serving was higher for all atypical situations modeled compared with the baseline model, and delay in drying had the greatest impact on risk.
[Mh] Termos MeSH primário: Carya/microbiologia
Contaminação de Alimentos/análise
Medição de Risco
[Mh] Termos MeSH secundário: Microbiologia de Alimentos
Seres Humanos
Intoxicação Alimentar por Salmonella/epidemiologia
Intoxicação Alimentar por Salmonella/prevenção & controle
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170817
[St] Status:MEDLINE
[do] DOI:10.4315/0362-028X.JFP-16-511


  2 / 138 MEDLINE  
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[PMID]:28648053
[Au] Autor:Gong Y; Pegg RB
[Ad] Endereço:Department of Food Science & Technology, College of Agricultural and Environmental Sciences, The University of Georgia , 100 Cedar Street, Athens, Georgia 30602, United States.
[Ti] Título:Separation of Ellagitannin-Rich Phenolics from U.S. Pecans and Chinese Hickory Nuts Using Fused-Core HPLC Columns and Their Characterization.
[So] Source:J Agric Food Chem;65(28):5810-5820, 2017 Jul 19.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:U.S. pecans and Chinese hickory nuts possess a wide array of phenolic constituents with potential health benefits including phenolic acids and proanthocyanidins. Only limited information is available, however, on their compositions. The present study optimized the separation performance and characterized the low-molecular-weight phenolic fractions of these nuts with C18 and pentafluorophenyl (PFP) fused-core LC columns by employing a kinetic approach. Although both types of reversed-phase columns demonstrated similar performance in general, the PFP column furnished greater plate numbers and superior peak shapes for the low-molecular-weight fractions as well as overall separations of ellagic acid derivatives. The high-molecular-weight fraction of pecans, analyzed by a 3-µm HILIC column, possessed more proanthocyanidins than the Chinese hickory nuts with dimers and trimers (31.4 and 18.34 mg/g crude extract, respectively) being present at the greatest levels. Chinese hickory nuts had lower proanthocyanidin content but possessed tetramers and pentamers at 4.46 and 4.01 mg/g crude extract, respectively.
[Mh] Termos MeSH primário: Carya/química
Taninos Hidrolisáveis/análise
Nozes/química
Fenóis/análise
Extratos Vegetais/análise
[Mh] Termos MeSH secundário: China
Cromatografia Líquida de Alta Pressão
Taninos Hidrolisáveis/isolamento & purificação
Fenóis/isolamento & purificação
Extratos Vegetais/isolamento & purificação
Proantocianidinas/análise
Proantocianidinas/isolamento & purificação
Estados Unidos
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Hydrolyzable Tannins); 0 (Phenols); 0 (Plant Extracts); 0 (Proanthocyanidins); 0 (ellagitannin); 18206-61-6 (proanthocyanidin)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170728
[Lr] Data última revisão:
170728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170627
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01597


  3 / 138 MEDLINE  
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[PMID]:28207311
[Au] Autor:Zhang G; Hu L; Melka D; Wang H; Laasri A; Brown EW; Strain E; Allard M; Bunning VK; Musser SM; Johnson R; Santillana Farakos S; Scott VN; Pouillot R; Doren JM; Hammack TS
[Ad] Endereço:U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, 5001 Campus Drive, College Park, Maryland 20740, USA.
[Ti] Título:Prevalence of Salmonella in Cashews, Hazelnuts, Macadamia Nuts, Pecans, Pine Nuts, and Walnuts in the United States.
[So] Source:J Food Prot;80(3):459-466, 2017 03.
[Is] ISSN:1944-9097
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nuts have been identified as a vector for salmonellosis. The objective of this project was to estimate the prevalence and contamination level of Salmonella in raw tree nuts (cashews, pecans, hazelnuts, macadamia nuts, pine nuts, and walnuts) at retail markets in the United States. A total of 3,656 samples of six types of tree nuts were collected from different types of retail stores and markets nationwide between October 2014 and October 2015. These samples were analyzed using a modified version of the Salmonella culture method from the U.S. Food and Drug Administration's Bacteriological Analytical Manual. Of the 3,656 samples collected and tested, 32 were culturally confirmed as containing Salmonella. These isolates represented 25 serotypes. Salmonella was not detected in pecans and in-shell hazelnuts. Salmonella prevalence estimates (and 95% confidence intervals) in cashews, shelled hazelnuts, pine nuts, walnuts, and macadamia nuts were 0.55% [0.15, 1.40], 0.35% [0.04, 1.20], 0.48% [0.10, 1.40], 1.20% [0.53, 2.40], and 4.20% [2.40, 6.90], respectively. The rates of Salmonella isolation from major or big chain supermarkets, small chain supermarkets, discount, variety, or drug stores, and online were 0.64% [0.38, 1.00], 1.60% [0.80, 2.90], 0.00% [0.00, 2.40], and 13.64% [2.90, 35.00], respectively (Cochran-Mantel-Haenszel test: P = 0.02). The rates of Salmonella isolation for conventional and organic nuts were not significantly different. Of the samples containing Salmonella, 60.7% had levels less than 0.003 most probable number (MPN)/g. The highest contamination level observed was 0.092 MPN/g. The prevalence and levels of Salmonella in these tree nut samples were comparable to those previously reported for similar foods.
[Mh] Termos MeSH primário: Nozes/microbiologia
Salmonella/isolamento & purificação
[Mh] Termos MeSH secundário: Anacardium
Carya
Corylus
Contaminação de Alimentos
Juglans
Macadamia
Prevalência
Estados Unidos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170622
[Lr] Data última revisão:
170622
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170217
[St] Status:MEDLINE
[do] DOI:10.4315/0362-028X.JFP-16-396


  4 / 138 MEDLINE  
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[PMID]:28126664
[Au] Autor:Bottari NB; Lopes LQ; Pizzuti K; Filippi Dos Santos Alves C; Corrêa MS; Bolzan LP; Zago A; de Almeida Vaucher R; Boligon AA; Giongo JL; Baldissera MD; Santos RC
[Ad] Endereço:Laboratory of Microbiology Research, Centro Universitário Franciscano, Santa Maria, RS, Brazil; Laboratory of Pharmacognosy, Centro Universitário Franciscano, Santa Maria, RS, Brazil.
[Ti] Título:Antimicrobial activity and phytochemical characterization of Carya illinoensis.
[So] Source:Microb Pathog;104:190-195, 2017 Mar.
[Is] ISSN:1096-1208
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Carya illinoensis is a widespread species, belonging to the Juglandaceae family, commonly known as Pecan. Popularly, the leaves have been used in the treatment of smoking as a hypoglycemic, cleansing, astringent, keratolytic, antioxidant, and antimicrobial agent. The following research aimed to identify for the first time the phytochemical compounds present in the leaves of C. illinoensis and carry out the determination of antimicrobial activity of aqueous and ethanolic extracts. The antimicrobial activity was tested against 20 microorganisms by determining the minimum inhibitory concentration (MIC). Phenolic acids (gallic acid and ellagic acid), flavonoids (rutin), and tannins (catechins and epicatechins) were identified by HPLC-DAD and may be partially responsible for the antimicrobial activity against Gram-positive, Gram-negative, and yeast. The results showed MIC values between 25 mg/mL and 0.78 mg/mL. The extracts were also able to inhibit the production of germ tubes by Candida albicans.
[Mh] Termos MeSH primário: Antifúngicos/análise
Antifúngicos/farmacologia
Bactérias/efeitos dos fármacos
Carya/química
Compostos Fitoquímicos/análise
Compostos Fitoquímicos/farmacologia
Leveduras/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antifúngicos/isolamento & purificação
Cromatografia Líquida de Alta Pressão
Flavonoides/análise
Flavonoides/isolamento & purificação
Flavonoides/farmacologia
Hidroxibenzoatos/análise
Hidroxibenzoatos/isolamento & purificação
Hidroxibenzoatos/farmacologia
Testes de Sensibilidade Microbiana
Compostos Fitoquímicos/isolamento & purificação
Folhas de Planta/química
Taninos/análise
Taninos/isolamento & purificação
Taninos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Flavonoids); 0 (Hydroxybenzoates); 0 (Phytochemicals); 0 (Tannins); 29656-58-4 (phenolic acid)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170413
[Lr] Data última revisão:
170413
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE


  5 / 138 MEDLINE  
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[PMID]:28121438
[Au] Autor:Mattison CP; Rai R; Settlage RE; Hinchliffe DJ; Madison C; Bland JM; Brashear S; Graham CJ; Tarver MR; Florane C; Bechtel PJ
[Ad] Endereço:Southern Regional Research Center, FPSQ, ARS, U.S. Department of Agriculture , 1100 Robert E. Lee Boulevard, New Orleans, Louisiana 70124, United States.
[Ti] Título:RNA-Seq Analysis of Developing Pecan (Carya illinoinensis) Embryos Reveals Parallel Expression Patterns among Allergen and Lipid Metabolism Genes.
[So] Source:J Agric Food Chem;65(7):1443-1455, 2017 Feb 22.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The pecan nut is a nutrient-rich part of a healthy diet full of beneficial fatty acids and antioxidants, but can also cause allergic reactions in people suffering from food allergy to the nuts. The transcriptome of a developing pecan nut was characterized to identify the gene expression occurring during the process of nut development and to highlight those genes involved in fatty acid metabolism and those that commonly act as food allergens. Pecan samples were collected at several time points during the embryo development process including the water, gel, dough, and mature nut stages. Library preparation and sequencing were performed using Illumina-based mRNA HiSeq with RNA from four time points during the growing season during August and September 2012. Sequence analysis with Trinotate software following the Trinity protocol identified 133,000 unigenes with 52,267 named transcripts and 45,882 annotated genes. A total of 27,312 genes were defined by GO annotation. Gene expression clustering analysis identified 12 different gene expression profiles, each containing a number of genes. Three pecan seed storage proteins that commonly act as allergens, Car i 1, Car i 2, and Car i 4, were significantly up-regulated during the time course. Up-regulated fatty acid metabolism genes that were identified included acyl-[ACP] desaturase and omega-6 desaturase genes involved in oleic and linoleic acid metabolism. Notably, a few of the up-regulated acyl-[ACP] desaturase and omega-6 desaturase genes that were identified have expression patterns similar to the allergen genes based upon gene expression clustering and qPCR analysis. These findings suggest the possibility of coordinated accumulation of lipids and allergens during pecan nut embryogenesis.
[Mh] Termos MeSH primário: Alérgenos/genética
Carya/embriologia
Carya/genética
Metabolismo dos Lipídeos
RNA de Plantas/genética
[Mh] Termos MeSH secundário: Alérgenos/metabolismo
Carya/metabolismo
RNA de Plantas/metabolismo
Estações do Ano
Sementes/enzimologia
Sementes/genética
Sementes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Allergens); 0 (RNA, Plant)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170526
[Lr] Data última revisão:
170526
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170126
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.6b04199


  6 / 138 MEDLINE  
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[PMID]:28078489
[Au] Autor:Wang Z; Huang R; Sun Z; Zhang T; Huang J
[Ad] Endereço:Nurturing Station for the State Key Laboratory of Subtropical Silviculture, School of Forestry and Biotechnology, Zhejiang Agriculture and Forestry University, Lin'an, Hangzhou, 311300, China.
[Ti] Título:Identification and profiling of conserved and novel microRNAs involved in oil and oleic acid production during embryogenesis in Carya cathayensis Sarg.
[So] Source:Funct Integr Genomics;17(2-3):365-373, 2017 May.
[Is] ISSN:1438-7948
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:MicroRNAs (miRNAs) are important regulators of plant development and fruit formation. Mature embryos of hickory (Carya cathayensis Sarg.) nuts contain more than 70% oil (comprising 90% unsaturated fatty acids), along with a substantial amount of oleic acid. To understand the roles of miRNAs involved in oil and oleic acid production during hickory embryogenesis, three small RNA libraries from different stages of embryogenesis were constructed. Deep sequencing of these three libraries identified 95 conserved miRNAs with 19 miRNA*s, 7 novel miRNAs (as well as their corresponding miRNA*s), and 26 potentially novel miRNAs. The analysis identified 15 miRNAs involved in oil and oleic acid production that are differentially expressed during embryogenesis in hickory. Among them, nine miRNA sequences, including eight conserved and one novel, were confirmed by qRT-PCR. In addition, 145 target genes of the novel miRNAs were predicted using a bioinformatic approach. Our results provide a framework for better understanding the roles of miRNAs during embryogenesis in hickory.
[Mh] Termos MeSH primário: Carya/genética
MicroRNAs/genética
[Mh] Termos MeSH secundário: Carya/embriologia
Desenvolvimento Embrionário
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170113
[St] Status:MEDLINE
[do] DOI:10.1007/s10142-016-0542-8


  7 / 138 MEDLINE  
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[PMID]:27838857
[Au] Autor:Marozzi G; Sánchez S; Benucci GM; Bonito G; Falini LB; Albertini E; Donnini D
[Ad] Endereço:Department of Agricultural, Food and Environmental Sciences, University of Perugia, 06121, Perugia, Italy.
[Ti] Título:Mycorrhization of pecan (Carya illinoinensis) with black truffles: Tuber melanosporum and Tuber brumale.
[So] Source:Mycorrhiza;27(3):303-309, 2017 Apr.
[Is] ISSN:1432-1890
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Pecan, Carya illinoinensis, is an economically important nut producing tree that can establish ectomycorrhizal symbiosis with a high diversity of fungi. In the southern USA, truffles (Tuber spp.) sometimes fruit prolifically in cultivated pecan orchards and regularly associate with pecan roots as ectomycorrhizae (ECMs). It has been demonstrated that some valuable European truffle species (e.g., Tuber borchii and Tuber aestivum) can form ECMs with pecan seedlings in nursery conditions. Thus, pecan may represent an attractive alternative host to forest trees for truffle growers given the potential for co-cropping truffles and pecans. To further explore the capacity of pecan to host truffle symbionts, pecan seedlings were inoculated with species of black truffles that are economically important in Europe, T. melanosporum and T. brumale. Ectomycorrhizae were characterized molecularly and their morphology was described in detail. Mycorrhization rates on pecan roots were assessed over a 2-year period. Tuber melanosporum and T. brumale produced well-formed ECMs with a level of root colonization in the first year of 37.3 and 34.5%, respectively. After 24 months, the level of mycorrhization increased for T. brumale (49.4%) and decreased for T. melanosporum (10.5%) inversely to that of non-target ECM greenhouse contaminants (e.g., Sphaerosporella brunnea, Trichophaea woolhopeia, Pulvinula constellatio). To assess whether mating types segregated in T. melanosporum as been reported for other host species, we amplified the mating-type locus from single T. melanosporum ECM belonging to different seedlings over a 2-year period. The two mating idiomorphs were nearly equally represented along the 2-year time span: MAT 1-1-1 decreased from 59.4% in the first year to 48.5% in the second year after inoculation. Data reported in this study add to knowledge on the mycorrhization of pecan trees with commercial truffles and has application to truffle and nut co-cropping systems.
[Mh] Termos MeSH primário: Carya/microbiologia
Micorrizas/fisiologia
Saccharomycetales/fisiologia
[Mh] Termos MeSH secundário: Fenótipo
Nódulos Radiculares de Plantas/microbiologia
Plântulas/microbiologia
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171011
[Lr] Data última revisão:
171011
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161114
[St] Status:MEDLINE
[do] DOI:10.1007/s00572-016-0743-y


  8 / 138 MEDLINE  
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[PMID]:27855649
[Au] Autor:Qiu L; Jiang B; Fang J; Shen Y; Fang Z; Rm SK; Yi K; Shen C; Yan D; Zheng B
[Ad] Endereço:Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Zhejiang A & F University, Linan, Hangzhou, 311300, People's Republic of China.
[Ti] Título:Analysis of transcriptome in hickory (Carya cathayensis), and uncover the dynamics in the hormonal signaling pathway during graft process.
[So] Source:BMC Genomics;17(1):935, 2016 11 17.
[Is] ISSN:1471-2164
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hickory (Carya cathayensis), a woody plant with high nutritional and economic value, is widely planted in China. Due to its long juvenile phase, grafting is a useful technique for large-scale cultivation of hickory. To reveal the molecular mechanism during the graft process, we sequenced the transcriptomes of graft union in hickory. RESULTS: In our study, six RNA-seq libraries yielded a total of 83,676,860 clean short reads comprising 4.19 Gb of sequence data. A large number of differentially expressed genes (DEGs) at three time points during the graft process were identified. In detail, 777 DEGs in the 7 d vs 0 d (day after grafting) comparison were classified into 11 enriched Gene Ontology (GO) categories, and 262 DEGs in the 14 d vs 0 d comparison were classified into 15 enriched GO categories. Furthermore, an overview of the PPI network was constructed by these DEGs. In addition, 20 genes related to the auxin-and cytokinin-signaling pathways were identified, and some were validated by qRT-PCR analysis. CONCLUSIONS: Our comprehensive analysis provides basic information on the candidate genes and hormone signaling pathways involved in the graft process in hickory and other woody plants.
[Mh] Termos MeSH primário: Carya/genética
Carya/metabolismo
Reguladores de Crescimento de Planta/metabolismo
Transdução de Sinais
Transcriptoma
[Mh] Termos MeSH secundário: Biologia Computacional/métodos
Citocininas/metabolismo
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Ontologia Genética
Sequenciamento de Nucleotídeos em Larga Escala
Ácidos Indolacéticos/metabolismo
Anotação de Sequência Molecular
Mapeamento de Interação de Proteínas
Mapas de Interação de Proteínas
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cytokinins); 0 (Indoleacetic Acids); 0 (Plant Growth Regulators)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171119
[Lr] Data última revisão:
171119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161119
[St] Status:MEDLINE


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[PMID]:27706743
[Au] Autor:Luo X; Li Z; Sun Z; Wan X
[Ad] Endereço:College of Forestry, Sichuan Agricultural University, Wenjang District, Sichuan Province, China.
[Ti] Título:Analysis of pecan cultivars Mahan and Western in East China.
[So] Source:Genet Mol Res;15(3), 2016 Sep 16.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Pecan (Carya illinoensis) has been introduced to East China for over one hundred years, but its planting is still only occurring at a small scale. The key limiting factor is its low yield. To enhance the yield pecan in East China, two pecan cultivars, Mahan and Western, were examined. Twenty traits describing phasic development, yield, nut quality, and cultural practice were investigated. We found that pecan cultivar Mahan gives a higher yield and nut quality than cultivar Western. We recommend interplanting of cultivar Pawnee to act as a pollinator tree. Appropriate cultivation practices that can be implemented to enhance fruit yield of cultivars Mahan and Western include soil-applied paclobutrazol (PBZ) at certain concentrations, pinching, and supplementary pollination. For example, the addition of 1.25 g/m of PBZ inhibits pecan branch growth and stimulates short bearing branches, which promotes fruit yield. We found that soil-applied PBZ reached optimal performance 82 days after application. A pinching length of 40 cm resulted in a fruit yield increase. In addition, grafting and transplantation may promote male flowering, but delays female flowering. These cultural practices may provide insights that can be used to improve pecan cultivation in East China.
[Mh] Termos MeSH primário: Carya/crescimento & desenvolvimento
Frutas/crescimento & desenvolvimento
Polinização/efeitos dos fármacos
[Mh] Termos MeSH secundário: Carya/efeitos dos fármacos
China
Produtos Agrícolas/efeitos dos fármacos
Frutas/efeitos dos fármacos
Triazóis/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Triazoles); 6PLV42R3ZA (paclobutrazol)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170221
[Lr] Data última revisão:
170221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161006
[St] Status:MEDLINE
[do] DOI:10.4238/gmr.15038732


  10 / 138 MEDLINE  
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[PMID]:27706736
[Au] Autor:Walker C; Muniz MF; Rolim JM; Martins RR; Rosenthal VC; Maciel CG; Mezzomo R; Reiniger LR
[Ad] Endereço:Departamento de Defesa Fitossanitária, Universidade Federal de Santa Maria, Santa Maria, RS, Brasil clairwalker@gmail.com.
[Ti] Título:Morphological and molecular characterization of Cladosporium cladosporioides species complex causing pecan tree leaf spot.
[So] Source:Genet Mol Res;15(3), 2016 Sep 16.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:The objective of this study was to characterize species of the Cladosporium cladosporioides complex isolated from pecan trees (Carya illinoinensis) with symptoms of leaf spot, based on morphological and molecular approaches. Morphological attributes were assessed using monosporic cultures on potato dextrose agar medium, which were examined for mycelial growth, sporulation, color, and conidia and ramoconidia size. Molecular characterization comprised isolation of DNA and subsequent amplification of the translation elongation factor 1α (TEF-1α) region. Three species of the C. cladosporioides complex were identified: C. cladosporioides, Cladosporium pseudocladosporioides, and Cladosporium subuliforme. Sporulation was the most important characteristic differentiating species of this genus. However, morphological features must be considered together with molecular analysis, as certain characters are indistinguishable between species. TEF-1αcan be effectively used to identify and group isolates belonging to the C. cladosporioides complex. The present study provides an important example of a methodology to ascertain similarity between isolates of this complex causing leaf spot in pecan trees, which should facilitate future pathogenicity studies.
[Mh] Termos MeSH primário: Carya/crescimento & desenvolvimento
Fator 1 de Elongação de Peptídeos/genética
Doenças das Plantas/genética
Folhas de Planta/genética
[Mh] Termos MeSH secundário: Carya/genética
Carya/microbiologia
Cladosporium/genética
Cladosporium/patogenicidade
Filogenia
Doenças das Plantas/microbiologia
Folhas de Planta/crescimento & desenvolvimento
Folhas de Planta/microbiologia
Esporos Fúngicos/genética
Esporos Fúngicos/patogenicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Peptide Elongation Factor 1)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170221
[Lr] Data última revisão:
170221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161006
[St] Status:MEDLINE
[do] DOI:10.4238/gmr.15038714



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