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Pesquisa : B01.650.940.800.575.912.250.859.844.500 [Categoria DeCS]
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[PMID]:28692289
[Au] Autor:De Leo M; Peruzzi L; Granchi C; Tuccinardi T; Minutolo F; De Tommasi N; Braca A
[Ad] Endereço:Dipartimento di Farmacia, Università di Pisa , Via Bonanno 6 and 33, 56126 Pisa, Italy.
[Ti] Título:Constituents of Polygala flavescens ssp. flavescens and Their Activity as Inhibitors of Human Lactate Dehydrogenase.
[So] Source:J Nat Prod;80(7):2077-2087, 2017 Jul 28.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Four new flavonol glycosides (1-4), two oligosaccharides (5, 6), one α-ionone (7), and three triterpenoid saponins (8-10), together with four known secondary metabolites (11-14), were isolated from the aerial parts of Polygala flavescens ssp. flavescens. All structures were elucidated on the basis of their spectroscopic and spectrometric data. The isolates were assayed for their inhibitory activity against isoform 5 of human lactate dehydrogenase, and compound 11 (3,6'-di-O-sinapoylsucrose) showed an IC value of 90.4 µM. Modeling studies were carried out to suggest the putative interaction mode of compound 11 in the enzyme active site.
[Mh] Termos MeSH primário: Flavonóis/isolamento & purificação
Flavonóis/farmacologia
L-Lactato Desidrogenase/antagonistas & inibidores
Norisoprenoides/isolamento & purificação
Norisoprenoides/farmacologia
Polygala/química
Saponinas/isolamento & purificação
Saponinas/farmacologia
Sacarose/análogos & derivados
Sacarose/farmacologia
Triterpenos/isolamento & purificação
[Mh] Termos MeSH secundário: Flavonóis/química
Seres Humanos
Concentração Inibidora 50
Itália
Estrutura Molecular
Norisoprenoides/química
Ressonância Magnética Nuclear Biomolecular
Componentes Aéreos da Planta/química
Saponinas/química
Sacarose/química
Sacarose/isolamento & purificação
Triterpenos/química
Triterpenos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonols); 0 (Norisoprenoids); 0 (Saponins); 0 (Triterpenes); 57-50-1 (Sucrose); EC 1.1.1.27 (L-Lactate Dehydrogenase); I9V075M61R (alpha-ionone)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170711
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.7b00295


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[PMID]:28608948
[Au] Autor:Li J; Wang DD; Xu XS; Bai L; Peng B; Pu YJ; Tian HL; Qin XM; Zhang FS; Ma CG
[Ad] Endereço:Pharmacy Department, Shanxi Pharmaceutical Vocational College, Taiyuan, 030031, P. R. China.
[Ti] Título:Utilization of UPLC/Q-TOF-MS-Based Metabolomics and AFLP-Based Marker-Assisted Selection to Facilitate/Assist Conventional Breeding of Polygala tenuifolia.
[So] Source:Chem Biodivers;14(9), 2017 Sep.
[Is] ISSN:1612-1880
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:As one of the most important traditional Chinese medicine, the quality of Polygala tenuifolia is difficult to control and a new method must be established to facilitate/assist the breeding of P. tenuifolia. In this study, UPLC/Q-TOF-MS-based metabolomics analysis was performed to determine the chemical composition and screen metabolite biomarkers according to agronomic traits. A total of 29 compounds and 18 metabolite biomarkers were found. AFLP-based marker-assisted selection (MAS) was used to identify molecular marker bands and screen characteristic bands associated with specific agronomic traits. 184 bands and 76 characteristic AFLP bands were found. The correlation network between compounds and characteristic AFLP bands was built, so we may directly breed certain P. tenuifolia herbs with special agronomic traits (or characteristic AFLP bands), which exhibit specific pharmacological functions depending on the content of the active compounds. The proposed method of metabolomics coupled with MAS could facilitate/assist the breeding of P. tenuifolia.
[Mh] Termos MeSH primário: Análise do Polimorfismo de Comprimento de Fragmentos Amplificados
Metaboloma
Melhoramento Vegetal
Polygala/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Análise por Conglomerados
DNA de Plantas/genética
Espectrometria de Massas
Metabolômica
Raízes de Plantas/genética
Raízes de Plantas/crescimento & desenvolvimento
Raízes de Plantas/metabolismo
Polygala/genética
Polygala/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171006
[Lr] Data última revisão:
171006
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170614
[St] Status:MEDLINE
[do] DOI:10.1002/cbdv.201700163


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[PMID]:28106783
[Au] Autor:Sur B; Lee B; Yoon YS; Lim P; Hong R; Yeom M; Lee HS; Park H; Shim I; Lee H; Jang YP; Hahm DH
[Ad] Endereço:Acupuncture and Meridian Science Research Center, College of Korean Medicine, Kyung Hee University, Seoul 02447, Korea. surzeus@naver.com.
[Ti] Título:Extract of Polygala tenuifolia Alleviates Stress-Exacerbated Atopy-Like Skin Dermatitis through the Modulation of Protein Kinase A and p38 Mitogen-Activated Protein Kinase Signaling Pathway.
[So] Source:Int J Mol Sci;18(1), 2017 Jan 18.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Atopic dermatitis (AD) and stress create a vicious cycle: stress exacerbates atopic symptoms, and atopic disease elicits stress and anxiety. Targeting multiple pathways including stress and allergic inflammation is, therefore, important for treating AD. In this study, we investigated the remedial value of Willd. (PTW) for treating immobilization (IMO) stress-exacerbated atopy-like skin dermatitis and its underlying mechanism. Trimellitic anhydride (TMA) was applied to dorsal skin for sensitization and subsequently both ears for eliciting T-cell-dependent contact hypersensitivity in mice, which underwent 2 h-IMO stress and PTW administration for the latter 6 and 9 days in the ear exposure period of TMA, respectively. To elicit in vitro degranulation of human mast cell line-1 (HMC-1), 10 µM substance P (SP) and 200 nM corticotrophin-releasing factor (CRF) were sequentially added with 48 h-interval. PTW extract (500 µg/mL) was added 30 min before CRF treatment. IMO stress exacerbated TMA-induced scratching behavior by 252%, and increased their blood corticosterone levels by two-fold. Treatment with 250 mg/kg PTW significantly restored IMO stress-exacerbated scratching behavior and other indicators such as skin inflammation and water content, lymph node weights, and serum histamine and immunoglobulin E (lgE) levels. Furthermore, it also reversed TMA-stimulated expression of tumor necrosis factor (TNF)-α and interleukin (IL)-4 mRNAs in ear tissues. PTW significantly inhibited SP/CRF-stimulated degranulation of HMC-1 cells, subsequent tryptase secretion, and protein kinase A (PKA) activity. PTW also selectively inhibited p38 mitogen-activated protein kinase (MAPK) phosphorylation in SP/CRF-treated HMC-1 cells. PTW significantly inhibited HMC-1 cell degranulation and alleviated IMO stress-exacerbated atopic dermatitis symptoms by modulating the PKA/p38 MAPK signaling pathway.
[Mh] Termos MeSH primário: Proteínas Quinases Dependentes de AMP Cíclico/metabolismo
Dermatite Atópica/tratamento farmacológico
Sistema de Sinalização das MAP Quinases
Extratos Vegetais/uso terapêutico
Polygala/química
Estresse Psicológico/tratamento farmacológico
Proteínas Quinases p38 Ativadas por Mitógeno/metabolismo
[Mh] Termos MeSH secundário: Animais
Comportamento Animal
Linhagem Celular
Cromatografia Líquida de Alta Pressão
Dermatite Atópica/sangue
Dermatite Atópica/complicações
Orelha/patologia
Seres Humanos
Imobilização
Imunoglobulina E/sangue
Interleucina-4/genética
Interleucina-4/metabolismo
Sistema de Sinalização das MAP Quinases/efeitos dos fármacos
Masculino
Mastócitos/metabolismo
Camundongos
Camundongos Endogâmicos BALB C
Compostos Fitoquímicos/análise
Fitoterapia
Extratos Vegetais/farmacologia
Proteína Quinase C/metabolismo
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Pele/patologia
Espectrometria de Massas por Ionização por Electrospray
Estresse Psicológico/sangue
Estresse Psicológico/complicações
Fator de Necrose Tumoral alfa/genética
Fator de Necrose Tumoral alfa/metabolismo
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Phytochemicals); 0 (Plant Extracts); 0 (RNA, Messenger); 0 (Tumor Necrosis Factor-alpha); 059QF0KO0R (Water); 207137-56-2 (Interleukin-4); 37341-29-0 (Immunoglobulin E); EC 2.7.11.11 (Cyclic AMP-Dependent Protein Kinases); EC 2.7.11.13 (Protein Kinase C); EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170424
[Lr] Data última revisão:
170424
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170121
[St] Status:MEDLINE


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[PMID]:27659250
[Au] Autor:Hong F; Wang L; Wu SL; Tang HC; Sha O; Wai MSM; Yew DT
[Ad] Endereço:Fujian Provincial Key Laboratory of Pien Tze Huang Natural Medicine Research and Development (R & D), Zhangzhou Pien Tze Huang Pharmaceutical CO., LTD. Zhangzhou. China.
[Ti] Título:A Review of Three Commonly Used Herbs Which Enhance Memory and New Evidences Which Show Their Combination Could Improve Memory in Young Animals.
[So] Source:Mini Rev Med Chem;17(16):1537-1547, 2017.
[Is] ISSN:1875-5607
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: This review looks into the herbs Gingko biloba, Polygala tenuifolia, and Lycii fructus for their widely studied neuroprotective properties. In particular, we investigated memory enhancing effect of these herbs, and their potential synergetic effect on memory with new data. Sixmonth treated mice demonstrated shorter escape latency in water maze and shorter arrival time in a consolidated memory task. Immunochemistry showed evident increase in superoxide dismutase activities in the prefrontal cortex, implying protection against free radicals during aging. Discrete increase of catecholaminergic neurons was found in the prefrontal cortex, hippocampus, corpus striatum, and midbrain, suggesting better memory and better control on mood and behavior. Necrotic cells in the brain decreased as indicated by immunocytochemistry of lactic dehydrogenase. Terminal deoxynucleotidyl transferase dUTP nick end labeling showed no apoptotic cells in most brain areas in high dose group. Biochemistry revealed increase of dopaminergic cells in treatment groups at prefrontal cortex, and in the hippocampus and cerebellum of the high dose group. Most 6-month groups showed increase of serotonin in all three areas. For the high dose group, GABA increased in the hippocampus but not prefrontal cortex, which would help induce sleep at night. Protein kinase C increased in most groups at prefrontal cortex, hippocampus and cerebellum, signifying increase of possible signal transduction pathways for memory or other nervous activations. CONCLUSION: Our results intimate that the interaction of the three herbs exerts beneficial effects on memory, associated cognitive function, and necrosis. Future investigations based on the present data shall aid development of clinically relevant medication.
[Mh] Termos MeSH primário: Cognição/efeitos dos fármacos
Ginkgo biloba
Lycium
Memória/efeitos dos fármacos
Fármacos Neuroprotetores/farmacologia
Preparações de Plantas/farmacologia
Polygala
[Mh] Termos MeSH secundário: Animais
Encéfalo/efeitos dos fármacos
Encéfalo/fisiologia
Ginkgo biloba/química
Seres Humanos
Lycium/química
Fármacos Neuroprotetores/química
Preparações de Plantas/química
Plantas Medicinais/química
Polygala/química
Superóxido Dismutase/metabolismo
Ácido gama-Aminobutírico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Neuroprotective Agents); 0 (Plant Preparations); 56-12-2 (gamma-Aminobutyric Acid); EC 1.15.1.1 (Superoxide Dismutase)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160924
[St] Status:MEDLINE
[do] DOI:10.2174/1389557516666160919164352


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[PMID]:27238050
[Au] Autor:Altenhofen D; da Luz G; Frederico MJ; Venzke D; Brich M; Vigil S; Fröde TS; Linares CE; Pizzolatti MG; Silva FR
[Ad] Endereço:Departamento de Bioquímica, Centro de Ciências Biológicas, Universidade Federal de Santa Catarina, Campus Universitário, Trindade, Cx. Postal 5069, CEP: 88040-970, Florianópolis, Santa Catarina, Brazil.
[Ti] Título:Bis-Pyrano Prenyl Isoflavone Improves Glucose Homeostasis by Inhibiting Dipeptidyl Peptidase-4 in Hyperglycemic Rats.
[So] Source:J Cell Biochem;118(1):92-103, 2017 Jan.
[Is] ISSN:1097-4644
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Isoflavones widely distributed in plants prevent diabetes. This study investigated the in vivo and in vitro effect of 3',4'-dihydroxy-6″,6″,6″',6″'-tetramethylbis(pyrano[2″,3″:5,6::2″',3″':7,8]isoflavone (bis-pyrano prenyl isoflavone) on glucose homeostasis in hyperglycemic rats. The ethyl acetate fraction from aerial parts of Polygala molluginifolia that contain isoflavones was assayed on glucose tolerance, on in vitro maltase activity and on protein glycation. The isoflavone bis-pyrano prenyl isolated from this fraction was investigated on glucose homeostasis. The in vivo action of the isoflavone exhibits an anti-hyperglycemic effect by improving glucose tolerance, augmenting the liver glycogen, inhibiting maltase activity, and stimulating glucagon-like peptide-1 (GLP-1) and insulin secretion. The in vitro isoflavone inhibits dipeptidyl peptidase-4 (DPP-4) activity since the glucose tolerance was improved in the presence of the isoflavone as much as sitagliptin, an inhibitor of DPP-4. However, the co-incubation with isoflavone and sitagliptin exhibited an additive anti-hyperglycemic action. The isoflavone increased the GLP-1 faster than the positive hyperglycemic group, which shows that the intestine is a potential target. Thus, to clarify the main site of action in which isoflavone improves glucose balance, the in vitro mechanism of action of this compound was tested in intestine using calcium influx as a trigger for the signal pathways for GLP-1 secretion. The isoflavone stimulates calcium influx in intestine and its mechanism involves voltage-dependent calcium channels, phospholipase C, protein kinase C, and stored calcium contributing for GLP-1 secretion. In conclusion, the isoflavone regulates glycaemia by acting mainly in a serum target, the DPP-4 inhibitor. Furthermore, the long-term effect of isoflavone prevents protein glycation. J. Cell. Biochem. 118: 92-103, 2017. © 2016 Wiley Periodicals, Inc.
[Mh] Termos MeSH primário: Glicemia/metabolismo
Dipeptidil Peptidase 4/sangue
Inibidores da Dipeptidil Peptidase IV/farmacologia
Hiperglicemia/tratamento farmacológico
Isoflavonas/farmacologia
Polygala/química
[Mh] Termos MeSH secundário: Animais
Inibidores da Dipeptidil Peptidase IV/química
Peptídeo 1 Semelhante ao Glucagon/sangue
Hiperglicemia/sangue
Insulina/sangue
Isoflavonas/química
Masculino
Ratos
Ratos Wistar
Fosfato de Sitagliptina/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Dipeptidyl-Peptidase IV Inhibitors); 0 (Insulin); 0 (Isoflavones); 89750-14-1 (Glucagon-Like Peptide 1); EC 3.4.14.5 (DPP4 protein, rat); EC 3.4.14.5 (Dipeptidyl Peptidase 4); TS63EW8X6F (Sitagliptin Phosphate)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160531
[St] Status:MEDLINE
[do] DOI:10.1002/jcb.25614


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[PMID]:27912882
[Au] Autor:Cao Q; Jiang Y; Cui SY; Tu PF; Chen YM; Ma XL; Cui XY; Huang YL; Ding H; Song JZ; Yu B; Sheng ZF; Wang ZJ; Xu YP; Yang G; Ye H; Hu X; Zhang YH
[Ad] Endereço:Department of pharmacology, Peking University, School of Basic Medical Science, 38 Xueyuan Road, Beijing, 100191, China.
[Ti] Título:Tenuifolin, a saponin derived from Radix Polygalae, exhibits sleep-enhancing effects in mice.
[So] Source:Phytomedicine;23(14):1797-1805, 2016 Dec 15.
[Is] ISSN:1618-095X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Radix Polygalae, the dried root of Polygala tenuifolia, has been extensively used as a traditional Chinese medicine for promoting intelligence and tranquilization. Polygalasaponins extracted from the root of P. tenuifolia possess evident anxiolytic and sedative-hypnotic activities. Previous studies have reported that tenuifolin was a major constituent of polygalasaponins. PURPOSE: The currently study aims to investigate the hypnotic effect and possible mechanism of tenuifolin in freely moving mice. DESIGN/METHODS: The hypnotic effects of tenuifolin (20, 40 and 80mg/kg, p.o.) were assessed by electroencephalographic (EEG) and electromyographic (EMG) analysis. Double-staining immunohistochemistry test was performed to evaluate the neuronal activity of sleep-wake regulating brain areas. High performance liquid chromatograph- electrochemical detection (HPLC-ECD) and ultrafast liquid chromatography-mass spectrometry (UFLC-MS) were used for the detection of neurotransmitters. Locomotor activity was measured by Open-field Test. RESULTS: Tenuifolin at doses of 40 and 80mg/kg (p.o.) significantly prolonged the total sleep time by increasing the amount of non-rapid eye movement (NREM) and rapid eye movement (REM) sleep, associated with the significant increase in the bouts of episodes respectively. After administration of tenuifolin, the cortical EEG power spectral densities during NREM and REM sleep were similar to that of natural sleep (vehicle) and thus compatible with physiological sleep. Double-immunohistochemistry staining test showed that tenuifolin increased the c-Fos positive ratios of GABAergic NREM sleep-promoting neurons in ventrolateral preoptic area (VLPO), cholinergic REM sleep-promoting neurons in laterodorsal tegmental area (LDT) and pontomesencephalic tegmental area (PPT) and decreased the c-Fos positive ratios in wake-promoting neurons (locus coeruleus (LC) and perifornical area (Pef)). Neurotransmitter detections revealed that tenuifolin significantly reduced the noradrenaline (NA) levels in LC, VLPO, PPT and LDT, elevated the GABA levels in VLPO, LC and Pef and increased the acetylcholine (Ach) levels in LDT and PPT. In addition, tenuifolin did not cause any change to locomotor activity. CONCLUSION: Taken together, these results provide the first experimental evidence of the significant sleep-enhancing effect of tenuifolin in mice. This effect appears to be mediated, at least in part, by the activation of GABAergic systems and/or by the inhibition of noradrenergic systems. Moreover, this study adds new scientific evidence and highlights the therapeutic potential of the medicinal plant P. tenuifolia in the development of phytomedicines with hypnotic properties.
[Mh] Termos MeSH primário: Encéfalo/efeitos dos fármacos
Diterpenos Caurânicos/farmacologia
Hipnóticos e Sedativos/farmacologia
Extratos Vegetais/farmacologia
Polygala/química
Saponinas/farmacologia
Sono/efeitos dos fármacos
[Mh] Termos MeSH secundário: Acetilcolina/metabolismo
Animais
Ansiolíticos/farmacologia
Encéfalo/metabolismo
Eletroencefalografia
Masculino
Camundongos Endogâmicos ICR
Neurotransmissores/metabolismo
Raízes de Plantas
Proteínas Proto-Oncogênicas c-fos/metabolismo
Sono REM/efeitos dos fármacos
Ácido gama-Aminobutírico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Anxiety Agents); 0 (Diterpenes, Kaurane); 0 (Hypnotics and Sedatives); 0 (Neurotransmitter Agents); 0 (Plant Extracts); 0 (Proto-Oncogene Proteins c-fos); 0 (Saponins); 0 (tenuifolin); 56-12-2 (gamma-Aminobutyric Acid); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161204
[St] Status:MEDLINE


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[PMID]:27618865
[Au] Autor:Lee JH; Lee JJ; Cho WK; Yim NH; Kim HK; Yun B; Ma JY
[Ad] Endereço:Korean Medicine (KM) Application Center, Korea Institute of Oriental Medicine, Daegu, 700-300, Republic of Korea.
[Ti] Título:KBH-1, an herbal composition, improves hepatic steatosis and leptin resistance in high-fat diet-induced obese rats.
[So] Source:BMC Complement Altern Med;16:355, 2016 Sep 13.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: KBH-1 is an herbal mixture of Saururus chinensis, Curcuma longa and Polygala tenuifolia. Each herb has been reported to have various pharmaceutical activities; however, the synergistic effect of this herbal composition on obesity has not yet been determined. We investigated the alleviation effect of KBH-1 and its possible molecular mechanism in obesity-induced hepatic steatosis and leptin resistance in the hypothalamus. METHODS: We used HepG2 cells, primary neuronal cells and a high-fat diet (HFD)-induced obesity rat model to determine the effect of KBH-1 in vitro and in vivo on hepatic steatosis and leptin resistance accompanied by obesity. To identify the alleviation effect on lipid accumulation, HepG2 cells stimulated by FFA were stained with Oil Red O; in addition, immunoblotting and qPCR were performed to determine the effect of KBH-1 on the activation of proteins and nuclear enzymes in HepG2 cells and the steatotic liver of HFD-induced obesity rats. To examine the effect of KBH-1 on the leptin resistance of the hypothalamus and its possible molecular mechanism, we examined the effect of KBH-1 on the activation of the leptin resistance-related protein in primary cultured cortical neuron cells and the hypothalamus of an HFD-induced obesity rat model. In addition, we used HPLC analysis to identify the standard compound of KBH-1. RESULTS: KBH-1 not only suppressed the lipid deposition in HepG2 cells exposed to free fatty acids (FFA) but also significantly down-regulated major factors in lipogenesis and up-regulated major factors in lipolysis. Similarly, in a HFD-induced obesity model, KBH-1 improved hepatic steatosis by alleviating the effects on lipogenic genes and kinases. In addition, KBH-1 significantly improved the leptin-mediated signals impaired by obesity or FFA in the obesity model and primary cultured cortical neuron cells. In addition, KBH-1 was analyzed to include six standard compounds using HPLC analysis, among these compounds, onji-saponin B and curcumin were potently suppressed the level of triglycerides. CONCLUSIONS: KBH-1 exhibits alleviating effects by improving hepatic steatosis and leptin resistance by up-regulating the activation of AMPK and suppressing the expression of PPARγ. These findings show the potential of KBH-1 as a functional food supplement or preventive agent in the treatment of obesity.
[Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/farmacologia
Medicamentos de Ervas Chinesas/uso terapêutico
Fígado Gorduroso/tratamento farmacológico
Fígado Gorduroso/metabolismo
Resistência à Insulina
Leptina/metabolismo
[Mh] Termos MeSH secundário: Animais
Curcuma/química
Dieta Hiperlipídica
Células Hep G2
Seres Humanos
Masculino
Polygala/química
Ratos
Ratos Sprague-Dawley
Saururaceae/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Drugs, Chinese Herbal); 0 (Leptin)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170222
[Lr] Data última revisão:
170222
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160914
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-016-1265-z


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[PMID]:27491751
[Au] Autor:Silva DF; Alves CQ; Brandão HN; David JM; David JP; Silva RL; Franchin M; Cunha TM; Martins FT; Oliveira CM
[Ad] Endereço:Departamento de Saúde, Universidade Estadual de Feira de Santana, 44036-900 Feira de Santana, BA, Brazil.
[Ti] Título:Poligalen, a new coumarin from Polygala boliviensis, reduces the release of TNF and IL-6 independent of NF-kB downregulation.
[So] Source:Fitoterapia;113:139-43, 2016 Sep.
[Is] ISSN:1873-6971
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:An unusually substituted coumarin, named poligalen, was isolated from a chloroform extract of the aerial parts of Polygala boliviensis. This coumarin was identified by one- and two-dimensional NMR techniques, and the structure of the compound was confirmed by X-ray diffraction. Poligalen exhibits immunomodulatory effects, reducing the levels of IL-6 and TNF after LPS stimulation in peritoneal macrophages. However, poligalen potentiates NF-kB activation.
[Mh] Termos MeSH primário: Cumarínicos/química
Interleucina-6/metabolismo
Macrófagos Peritoneais/efeitos dos fármacos
Polygala/química
Fator de Necrose Tumoral alfa/metabolismo
[Mh] Termos MeSH secundário: Animais
Cumarínicos/isolamento & purificação
Regulação para Baixo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Estrutura Molecular
NF-kappa B/metabolismo
Componentes Aéreos da Planta/química
Cultura Primária de Células
Células RAW 264.7
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Coumarins); 0 (Interleukin-6); 0 (NF-kappa B); 0 (Tumor Necrosis Factor-alpha); 0 (poligalen)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170131
[Lr] Data última revisão:
170131
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160806
[St] Status:MEDLINE


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[PMID]:27450426
[Au] Autor:Yu D; Pu W; Li D; Wang D; Liu Q; Wang Y
[Ad] Endereço:College of Forestry, Northwest A&F University, Yangling, Shaanxi, 712100, P. R. China.
[Ti] Título:Phenolic Compounds and Antioxidant Activity of Different Organs of Potentilla fruticosa L. from Two Main Production Areas of China.
[So] Source:Chem Biodivers;13(9):1140-1148, 2016 Sep.
[Is] ISSN:1612-1880
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:This report compared the phenolic compounds and antioxidant activity of the leaves, flowers, and stems of Potentilla fruticosa L. collected from two main production areas of P. R. China (Taibai Mountains and the Qinghai Huzhu Northern Mountains). The results indicated that there were significant differences in the phenol contents and antioxidant activities among the different organs and between the two productions. High-performance liquid-chromatography analysis indicated that hyperoside, (+)-catechin, ellagic acid, and rutin were the primary compounds in leaves and flowers; for stems, the content of six phenolic compounds, from two productions, were the lowest. The 1,1-diphenyl-2-picryl hydrazyl (DPPH), 2,2-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) di-ammonium salt (ABTS), ferric reducing power (FRAP), lipid peroxidation assays, and microbial test system (MTS) were used to evaluate the antioxidant activity. The results demonstrated that the leaves from two productions exhibited powerful antioxidant activity than other organs, which did not significantly differ from that of the positive control (rutin), followed by the flowers and stems. The correlation between the content of phytochemicals and the antioxidant activities of different organs showed that the total phenol, tannin, hyperoside, and (+)-catechin contents may influence the antioxidant activity, and these compounds can be used as markers for the quality control of P. fruticosa.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Flores/química
Fenóis/farmacologia
Folhas de Planta/química
Caules de Planta/química
Potentilla/química
[Mh] Termos MeSH secundário: Antioxidantes/química
Antioxidantes/isolamento & purificação
China
Cromatografia Líquida de Alta Pressão
Escherichia coli/efeitos dos fármacos
Peroxidação de Lipídeos/efeitos dos fármacos
Fenóis/química
Fenóis/isolamento & purificação
Polygala/efeitos dos fármacos
Polygala/microbiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Phenols)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170118
[Lr] Data última revisão:
170118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160725
[St] Status:MEDLINE
[do] DOI:10.1002/cbdv.201500512


  10 / 206 MEDLINE  
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[PMID]:27180981
[Au] Autor:Zhou H; Xue W; Chu SF; Wang ZZ; Li CJ; Jiang YN; Luo LM; Luo P; Li G; Zhang DM; Chen NH
[Ad] Endereço:State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica & Neuroscience Center, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, China.
[Ti] Título:Polygalasaponin XXXII, a triterpenoid saponin from Polygalae Radix, attenuates scopolamine-induced cognitive impairments in mice.
[So] Source:Acta Pharmacol Sin;37(8):1045-53, 2016 Aug.
[Is] ISSN:1745-7254
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:AIM: Recent studies show that the extract of a Chinese herb Polygalae Radix exerts cognition-enhancing actions in rats and humans. The aim of this study was to characterize the pharmacological profiles of active compounds extracted from Polygalae Radix. METHODS: Two fractions P3 and P6 and two compounds PTM-15 and polygalasaponin XXXII (PGS32) were prepared. Neuroprotective effects were evaluated in primary cortical neurons exposed to high concentration glutamate, serum deficiency or H2O2. Anti-dementia actions were assessed in scopolamine-induced amnesia in mice using step-through avoidance tests and channel water maze tests. After conducting the channel water maze tests, TrkB phosphorylation in mouse hippocampus was detected using Western blotting. Long-term potentiation (LTP) was induced in the dentate gyrus in adult rats; PGS32 (5 µL 400 µmol/L) was injected into the lateral cerebral ventricle 20 min after high frequency stimulation (HFS). RESULTS: Compared to the fraction P6, the fraction P3 showed more prominent neuroprotective effects in vitro and cognition-enhancing effects in scopolamine-induced amnesia in mice. One active compound PGS32 in the fraction P3 exerted potent cognition-enhancing action: oral administration of PGS32 (0.125 mg·kg(-1)·d(-1)) for 19 days abolished scopolamine-induced memory impairment in mice. Furthermore, PGS32 (0.5 and 2 mg·kg(-1)·d(-1)) significantly stimulated the phosphorylation of TrkB in the hippocampus. Intracerebroventricular injection of PGS32 significantly enhanced HFS-induced LTP in the dentate gyrus of rats. CONCLUSION: PGS32 attenuates scopolamine-induced cognitive impairments in mice, suggesting that it has a potential for the treatment of cognitive dysfunction and dementia.
[Mh] Termos MeSH primário: Disfunção Cognitiva/induzido quimicamente
Disfunção Cognitiva/tratamento farmacológico
Fármacos Neuroprotetores/farmacologia
Polygala/química
Saponinas/farmacologia
Hidrobrometo de Escopolamina/farmacologia
Triterpenos/farmacologia
[Mh] Termos MeSH secundário: Animais
Aprendizagem da Esquiva/efeitos dos fármacos
Córtex Cerebral/efeitos dos fármacos
Giro Denteado/efeitos dos fármacos
Ácido Glutâmico/farmacologia
Hipocampo/efeitos dos fármacos
Peróxido de Hidrogênio/farmacologia
Potenciação de Longa Duração/efeitos dos fármacos
Masculino
Aprendizagem em Labirinto/efeitos dos fármacos
Camundongos
Neurônios/efeitos dos fármacos
Fármacos Neuroprotetores/isolamento & purificação
Cultura Primária de Células
Proteínas Tirosina Quinases/metabolismo
Ratos
Saponinas/isolamento & purificação
Hidrobrometo de Escopolamina/antagonistas & inibidores
Triterpenos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Neuroprotective Agents); 0 (Saponins); 0 (Triterpenes); 0 (polygalasaponin XXXII); 3KX376GY7L (Glutamic Acid); 451IFR0GXB (Scopolamine Hydrobromide); BBX060AN9V (Hydrogen Peroxide); EC 2.7.10.1 (Protein-Tyrosine Kinases)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170801
[Lr] Data última revisão:
170801
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160517
[St] Status:MEDLINE
[do] DOI:10.1038/aps.2016.17



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