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[PMID]: | 26739110 |
[Au] Autor: | Akin D; Durak Y; Uysal A; Gunes E; Aladag MO |
[Ad] Endereço: | a Meram Faculty of Medicine, Department of Anatomy , Necmettin Erbakan University , Konya , Turkey . |
[Ti] Título: | Assessment of antimutagenic action of Celtis glabrata Steven ex Planch. (Cannabaceae) extracts against base pair exchange and frame shift mutations on Salmonella typhimurium TA98 and TA100 strains by Ames test. |
[So] Source: | Drug Chem Toxicol;39(3):312-21, 2016. | [Is] ISSN: | 1525-6014 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | CONTEXT: Celtis glabrata is used in Turkey for the treatment of various health disorders. OBJECTIVE: The acetone, chloroform, ethanol, and methanol extracts of C. glabrata leaf, fruit, and seed were investigated to evaluate their antimutagenic activities. MATERIAL AND METHODS: The antimutagenicity of these extracts was determined by Ames test against mutagens (4-nitro-O-phenylenediamine, 2-aminofluorene (2-AF), and sodium azide (SA)). The extracts were used at concentrations between 5 and 0.005 mg/plate. RESULTS: The ethanol extracts of leaves exhibited strong antimutagenicity (70%) against 2-AF with S9 at 5 mg/plate on TA98. But methanol (61%, 53%) and acetone (53%, 52%) also revealed strong inhibition rates at concentrations of ≥ 0.5 mg/plate. Among the extracts, the highest activity (96%) was obtained from acetone extract against SA without S9, followed by chloroform extract (91%) at a dose of 5 mg/plate on TA100 with S9. Ethanol (without S9) and chloroform (with S9) extracts showed strong antimutagenicity at all doses. Exception of chloroform and acetone (without S9), all fruit extracts (with/without S9) manifested strong antimutagenicity at doses of ≥ 0.5 mg/plate on TA98 strain. Ethanol extracts revealed 68% inhibition against 2-AF on TA98. Acetone and ethanol extracts manifested 84% and 82% inhibition against SA on TA100, respectively. All the extracts of seeds revealed strong inhibition against 2-AF at ≥ 0.5 mg/plate doses on TA98, but acetone extract showed excellent antimutagenicity (94%). Moreover, the chloroform (74, 73, 63, 54%), acetone (74, 72, 70, 65%) and methanol (74, 67, 63, 61%) extracts of seeds revealed strong antimutagenic activity on TA100 against SA with S9. DISCUSSION AND CONCLUSION: This plant may be natural source of antimutagenic agents. |
[Mh] Termos MeSH primário: |
Antimutagênicos/farmacologia Pareamento Incorreto de Bases/efeitos dos fármacos Cannabaceae/química Mutação da Fase de Leitura/efeitos dos fármacos Extratos Vegetais/farmacologia Salmonella typhimurium/efeitos dos fármacos
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[Mh] Termos MeSH secundário: |
Antimutagênicos/envenenamento Frutas/química Mutagênicos/toxicidade Extratos Vegetais/isolamento & purificação Folhas de Planta/química Salmonella typhimurium/genética Sementes/química
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[Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T |
[Nm] Nome de substância:
| 0 (Antimutagenic Agents); 0 (Mutagens); 0 (Plant Extracts) |
[Em] Mês de entrada: | 1612 |
[Cu] Atualização por classe: | 161231 |
[Lr] Data última revisão:
| 161231 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 160108 |
[St] Status: | MEDLINE |
[do] DOI: | 10.3109/01480545.2015.1121273 |
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