Base de dados : MEDLINE Pesquisa : B01.650.940.800.575.912.250.859.937.249.249 [Categoria DeCS] Referências encontradas : 148 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 28545532 [Au] Autor: Kuete V; Fokou FW; Karaosmanoglu O; Beng VP; Sivas H [Ad] Endereço: Department of Biochemistry, Faculty of Science, University of Dschang, Dschang, Cameroon. kuetevictor@yahoo.fr. [Ti] Título: Cytotoxicity of the methanol extracts of Elephantopus mollis, Kalanchoe crenata and 4 other Cameroonian medicinal plants towards human carcinoma cells. [So] Source: BMC Complement Altern Med;17(1):280, 2017 May 25. [Is] ISSN: 1472-6882 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Cancer still constitutes one of the major health concerns globally, causing serious threats on patients, their families, and the healthcare system. METHODS: In this study, the cytotoxicity of the methanol extract of Elephantopus mollis whole plant (EMW), Enantia chlorantha bark (ECB), Kalanchoe crenata leaves (KCL), Lophira alata bark (LAB), Millettia macrophylla leaves (MML) and Phragmanthera capitata leaves (PCL) towards five human solid cancer cell lines and normal CRL2120 fibroblasts, was evaluated. Extracts were subjected to qualitative chemical screening of their secondary metabolite contents using standard methods. The cytotoxicity of samples was evaluated using neutral red uptake (NR) assay meanwhile caspase activation was detected by caspase-Glo assay. Flow cytometry was used to analyze the cell cycle distribution and the mitochondrial membrane potential (MMP) whilst spectrophotometry was used to measure the levels of reactive oxygen species (ROS). RESULTS: Phytochemical analysis revealed the presence of polyphenols, triterpenes and sterols in all extracts. The IC values of the best samples ranged from 3.29 µg/mL (towards DLD-1 colorectal adenocarcinoma cells) to 24.38 µg/mL (against small lung cancer A549 cells) for EMW, from 2.33 µg/mL (mesothelioma SPC212 cells) to 28.96 µg/mL (HepG2 hepatocarcinoma) for KCL, and from 0.04 µg/mL (towards SPC212 cells) to 0.55 µg/mL (towards A549 cells) for doxorubicin. EMW induced apoptosis in MCF-7 cells mediated by MMP loss and increased ROS production whilst KCL induced apoptosis via ROS production. CONCLUSION: This study provides evidences of the cytotoxicity of the tested plant extract and highlights the good activity of Elephantopus mollis and Kalanchoe crenata. They deserve more exploration to develop novel cytotoxic drugs. [Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia Asteraceae/química Kalanchoe/química Extratos Vegetais/farmacologia Plantas Medicinais/química [Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/isolamento & purificação Carcinoma/tratamento farmacológico Carcinoma/metabolismo Carcinoma/fisiopatologia Ciclo Celular/efeitos dos fármacos Linhagem Celular Tumoral Seres Humanos Potencial da Membrana Mitocondrial/efeitos dos fármacos Extratos Vegetais/isolamento & purificação Espécies Reativas de Oxigênio/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Plant Extracts); 0 (Reactive Oxygen Species) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170822 [Lr] Data última revisão: 170822 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170527 [St] Status: MEDLINE [do] DOI: 10.1186/s12906-017-1793-1

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[PMID]: 28395583 [Au] Autor: Cryer M; Lane K; Greer M; Cates R; Burt S; Andrus M; Zou J; Rogers P; Hansen MD; Burgado J; Panayampalli SS; Day CW; Smee DF; Johnson BF [Ad] Endereço: a Department of Microbiology and Molecular Biology , Brigham Young University , Provo , UT , USA. [Ti] Título: Isolation and identification of compounds from Kalanchoe pinnata having human alphaherpesvirus and vaccinia virus antiviral activity. [So] Source: Pharm Biol;55(1):1586-1591, 2017 Dec. [Is] ISSN: 1744-5116 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: CONTEXT: Kalanchoe pinnata (Lam.) Pers. (Crassulaceae) is a succulent plant that is known for its traditional antivirus and antibacterial usage. OBJECTIVE: This work examines two compounds identified from the K. pinnata plant for their antivirus activity against human alphaherpesvirus (HHV) 1 and 2 and vaccinia virus (VACV). MATERIALS AND METHODS: Compounds KPB-100 and KPB-200 were isolated using HPLC and were identified using NMR and MS. Both compounds were tested in plaque reduction assay of HHV-2 wild type (WT) and VACV. Both compounds were then tested in virus spread inhibition and virus yield reduction (VYR) assays of VACV. KPB-100 was further tested in viral cytopathic effect (CPE) inhibition assay of HHV-2 TK-mutant and VYR assay of HHV-1 WT. RESULTS: KPB-100 and KPB-200 inhibited HHV-2 at IC values of 2.5 and 2.9 µg/mL, respectively, and VACV at IC values of 3.1 and 7.4 µg/mL, respectively, in plaque reduction assays. In virus spread inhibition assay of VACV KPB-100 and KPB-200 yielded IC values of 1.63 and 13.2 µg/mL, respectively, and KPB-100 showed a nearly 2-log reduction in virus in VYR assay of VACV at 20 µg/mL. Finally, KPB-100 inhibited HHV-2 TK- at an IC value of 4.5 µg/mL in CPE inhibition assay and HHV-1 at an IC of 3.0 µg/mL in VYR assay. DISCUSSION AND CONCLUSION: Both compounds are promising targets for synthetic optimization and in vivo study. KPB-100 in particular showed strong inhibition of all viruses tested. [Mh] Termos MeSH primário: Antivirais/farmacologia Kalanchoe/química Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Antivirais/administração & dosagem Antivirais/isolamento & purificação Cromatografia Líquida de Alta Pressão Efeito Citopatogênico Viral/efeitos dos fármacos Células HeLa Herpesvirus Humano 1/efeitos dos fármacos Herpesvirus Humano 2/efeitos dos fármacos Seres Humanos Concentração Inibidora 50 Espectroscopia de Ressonância Magnética Espectrometria de Massas Extratos Vegetais/administração & dosagem Extratos Vegetais/isolamento & purificação Vírus Vaccinia/efeitos dos fármacos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antiviral Agents); 0 (Plant Extracts) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171004 [Lr] Data última revisão: 171004 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170412 [St] Status: MEDLINE [do] DOI: 10.1080/13880209.2017.1310907

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[PMID]: 28326334 [Au] Autor: Lebedeva AA; Zakharchenko NS; Trubnikova EV; Medvedeva OA; Kuznetsova TV; Masgutova GA; Zylkova MV; Buryanov YI; Belous AS [Ad] Endereço: Russian Academy of Sciences, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino, Moscow Region, Russia. [Ti] Título: Bactericide, Immunomodulating, and Wound Healing Properties of Transgenic Synergize with Antimicrobial Peptide Cecropin P1 In Vivo. [So] Source: J Immunol Res;2017:4645701, 2017. [Is] ISSN: 2314-7156 [Cp] País de publicação: Egypt [La] Idioma: eng [Ab] Resumo: Procedure of manufacturing water extracts containing cecropin P1 (CecP1) from the formerly described transgenic plants is established. It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total protein in the extract. The water extract of + CecP1 exhibits favorable effect on healing of wounds infected with (equal to Cefazolin) and with a combination of with (better than Cefazolin). Wild-type extract exhibited evident microbicide activity against with but it was substantially strengthened in + CecP1 extract. extracts (both wild-type and transgenic) did not exhibit general toxicity and accelerated wound recovery. Due to immunomodulating activity, wild-type extract accelerated granulation of the wound bed and marginal epithelialization even better than + CecP1 extract. Immunomodulating and microbicide activity of synergizes with microbicide activity of CecP1 accelerating elimination of bacteria. [Mh] Termos MeSH primário: Anti-Infecciosos/uso terapêutico Kalanchoe/genética Peptídeos/uso terapêutico Extratos Vegetais/uso terapêutico Infecções por Pseudomonas/tratamento farmacológico Pseudomonas aeruginosa/fisiologia Proteínas Recombinantes/uso terapêutico Infecções Estafilocócicas/tratamento farmacológico Staphylococcus aureus/fisiologia Infecção dos Ferimentos/tratamento farmacológico [Mh] Termos MeSH secundário: Animais Cefazolina/uso terapêutico Seres Humanos Imunomodulação Masculino Peptídeos/genética Plantas Geneticamente Modificadas Ratos Ratos Wistar Proteínas Recombinantes/genética Suínos Cicatrização/efeitos dos fármacos [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Infective Agents); 0 (Peptides); 0 (Plant Extracts); 0 (Recombinant Proteins); 0 (cecropin P1-LI); IHS69L0Y4T (Cefazolin) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170407 [Lr] Data última revisão: 170407 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170323 [St] Status: MEDLINE [do] DOI: 10.1155/2017/4645701

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[PMID]: 28223215 [Au] Autor: Kolodziejczyk-Czepas J; Sieradzka M; Moniuszko-Szajwaj B; Pecio L; Ponczek MB; Nowak P; Stochmal A [Ad] Endereço: Department of General Biochemistry, Faculty of Biology and Environmental Protection, University of Lodz, Pomorska 141/143, 90-236 Lodz, Poland. Electronic address: joannak@biol.uni.lodz.pl. [Ti] Título: Bufadienolides from Kalanchoe daigremontiana as thrombin inhibitors-In vitro and in silico study. [So] Source: Int J Biol Macromol;99:141-150, 2017 Jun. [Is] ISSN: 1879-0003 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Thrombin is an active plasma coagulation factor II, critical for the formation of fibrin clot during blood coagulation. For that reason, this protein is also a crucial target for different anti-thrombotic therapies. The work is based on in vitro evaluation of the inhibitory effect of bufadienolide-rich fraction, isolated from roots of Kalanchoe daigremontiana (1-50µg/ml) on enzymatic properties of a serine proteinase - thrombin. The efficacy of the inhibition of amidolytic activity of thrombin (measured as a hydrolysis of the chromogenic substrate S-2238, Chromogenix) attained about 10 and 66%, respectively. The IC , established for the examined bufadienolide fraction was 2.79µg/ml, while the IC calculated for argatroban (reference compound) was 0.78µg/ml. Linearization conducted using Lineweaver-Burk plot indicated that the K. daigremontiana fraction contains compounds that are uncompetitive inhibitors of thrombin. K. daigremontiana fraction was also able to reduce the proteolytic activity of thrombin towards its physiological substrate, i.e. fibrinogen. Additionally, this study is supported by in silico analysis of interactions of the most common compounds, identified in the examined in Kalanchoe extract to crystal structure of this enzyme. [Mh] Termos MeSH primário: Bufanolídeos/farmacologia Simulação por Computador Kalanchoe/química Inibidores de Proteases/farmacologia Trombina/antagonistas & inibidores [Mh] Termos MeSH secundário: Amidas/química Amidas/metabolismo Bufanolídeos/química Bufanolídeos/metabolismo Simulação de Acoplamento Molecular Inibidores de Proteases/química Inibidores de Proteases/metabolismo Conformação Proteica Proteólise/efeitos dos fármacos Trombina/química Trombina/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Amides); 0 (Bufanolides); 0 (Protease Inhibitors); 29565-35-3 (bufadienolide); EC 3.4.21.5 (Thrombin) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170428 [Lr] Data última revisão: 170428 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170223 [St] Status: MEDLINE

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[PMID]: 27937097 [Au] Autor: Kouitcheu Mabeku LB; Eyoum Bille B; Tchouangueu TF; Nguepi E; Leundji H [Ad] Endereço: a Microbiology and Pharmacology Laboratory, Department of Biochemistry, Faculty of Science , University of Dschang , Dschang , Cameroon. [Ti] Título: Treatment of Helicobacter pylori infected mice with Bryophyllum pinnatum, a medicinal plant with antioxidant and antimicrobial properties, reduces bacterial load. [So] Source: Pharm Biol;55(1):603-610, 2017 Dec. [Is] ISSN: 1744-5116 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: CONTEXT: Bryophyllum pinnatum (Lam.) Kurz (Crassulaceae) is a plant known for its antiulcer properties. OBJECTIVE: This study evaluates the anti-Helicobacter pylori activity of Bryophyllum pinnutum methanol extract with a mouse model and its antioxidant properties. MATERIALS AND METHODS: Dried leaves of Bryophyllum pinnutum were extracted with methanol and ethyl acetate. Broth microdilution method was used to evaluate the anti-Helicobacter activity of extract samples in vitro. Swiss mice were inoculated with a suspension of Helicobacter pylori and divided into control group and four others that received 125, 250, 500 mg/kg of methanol extract or ciprofloxacin (500 mg/kg), respectively, for 7 days. Helicobacter pylori colonization and bacterial load of mouse stomach was assessed on day 1 and 7 post-treatment. The antioxidant activity of Bryophyllum pinnutum was evaluated through DPPH radical, hydroxyl radical and reducing power assay. RESULTS: Methanol extract showed a significant anti-Helicobacter activity with MIC and MBC values of 32 and 256 µg/mL, respectively. Bryophyllum pinnatum and ciprofloxacin reduced H. pylori colonization of gastric tissue from 100% to 17%. Bryophyllum pinnatum extract (85.91 ± 52.91 CFU) and standard (25.74 ± 16.15 CFU) also reduced significantly (p < 0.05) bacterial load of gastric mucosa as compared to untreated infected mice (11883 ± 1831 CFU). DPPH radical, hydroxyl radical and reducing power assays showed IC values of 25.31 ± 0.34, 55.94 ± 0.68 and 11.18 ± 0.74 µg/mL, respectively. DISCUSSION AND CONCLUSION: The data suggest that the methanol extract of Bryophyllum pinnatum could inhibit Helicobacter pylori growth, and may also acts as an antioxidant to protect gastric mucosa against reactive oxygen species. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Antioxidantes/farmacologia Mucosa Gástrica/efeitos dos fármacos Infecções por Helicobacter/tratamento farmacológico Helicobacter pylori/efeitos dos fármacos Kalanchoe/química Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Animais Antibacterianos/isolamento & purificação Antioxidantes/isolamento & purificação Carga Bacteriana Compostos de Bifenilo/química Modelos Animais de Doenças Mucosa Gástrica/microbiologia Infecções por Helicobacter/microbiologia Helicobacter pylori/patogenicidade Radical Hidroxila/química Metanol/química Camundongos Testes de Sensibilidade Microbiana Oxirredução Fitoterapia Picratos/química Extratos Vegetais/isolamento & purificação Folhas de Planta Plantas Medicinais Solventes/química Fatores de Tempo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents); 0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Picrates); 0 (Plant Extracts); 0 (Solvents); 3352-57-6 (Hydroxyl Radical); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl); Y4S76JWI15 (Methanol) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170313 [Lr] Data última revisão: 170313 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161213 [St] Status: MEDLINE

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[PMID]: 28033347 [Au] Autor: Fernandes JM; Félix-Silva J; da Cunha LM; Gomes JA; Siqueira EM; Gimenes LP; Lopes NP; Soares LA; Fernandes-Pedrosa MF; Zucolotto SM [Ad] Endereço: Grupo de Pesquisa em Produtos Naturais Bioativos (PNBio), Laboratório de Farmacognosia, Departamento de Farmácia, Universidade Federal do Rio Grande do Norte, (UFRN), Natal, Rio Grande do Norte, Brazil. [Ti] Título: Inhibitory Effects of Hydroethanolic Leaf Extracts of Kalanchoe brasiliensis and Kalanchoe pinnata (Crassulaceae) against Local Effects Induced by Bothrops jararaca Snake Venom. [So] Source: PLoS One;11(12):e0168658, 2016. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: The species Kalanchoe brasiliensis and Kalanchoe pinnata, both known popularly as "Saião," are used interchangeably in traditional medicine for their antiophidic properties. Studies evaluating the anti-venom activity of these species are scarce. This study aims to characterize the chemical constituents and evaluate the inhibitory effects of hydroethanolic leaf extracts of K. brasiliensis and K. pinnata against local effects induced by Bothrops jararaca snake venom. Thin Layer Chromatography (TLC) and High Performance Liquid Chromatography coupled with Diode Array Detection and Electrospray Mass Spectrometry (HPLC-DAD-MS/MS) were performed for characterization of chemical markers of the extracts from these species. For antiophidic activity evaluation, B. jararaca venom-induced paw edema and skin hemorrhage in mice were evaluated. In both models, hydroethanolic extracts (125-500 mg/kg) were administered intraperitoneally in different protocols. Inhibition of phospholipase enzymatic activity of B. jararaca was evaluated. The HPLC-DAD-MS/MS chromatographic profile of extracts showed some particularities in the chemical profile of the two species. K. brasileinsis exhibited major peaks that have UV spectra similar to flavonoid glycosides derived from patuletin and eupafolin, while K. pinnata showed UV spectra similar to flavonoids glycosides derived from quercetin and kaempferol. Both extracts significantly reduced the hemorrhagic activity of B. jararaca venom in pre-treatment protocol, reaching about 40% of inhibition, while only K. pinnata was active in post-treatment protocol (about 30% of inhibition). In the antiedematogenic activity, only K. pinnata was active, inhibiting about 66% and 30% in pre and post-treatment protocols, respectively. Both extracts inhibited phospholipase activity; however, K. pinnata was more active. In conclusion, the results indicate the potential antiophidic activity of Kalanchoe species against local effects induced by B. jararaca snake venom, suggesting their potential use as a new source of bioactive molecules against bothropic venom. [Mh] Termos MeSH primário: Bothrops Venenos de Crotalídeos/toxicidade Etanol/química Kalanchoe/química Extratos Vegetais/farmacologia Folhas de Planta/química [Mh] Termos MeSH secundário: Animais Edema/induzido quimicamente Edema/tratamento farmacológico Feminino Hemorragia/induzido quimicamente Hemorragia/tratamento farmacológico Masculino Camundongos Extratos Vegetais/uso terapêutico [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Crotalid Venoms); 0 (Plant Extracts); 3K9958V90M (Ethanol) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170718 [Lr] Data última revisão: 170718 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161230 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0168658

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[PMID]: 27682168 [Au] Autor: Shevchuk TV; Zakharchenko NS; Tarlachkov SV; Furs OV; Dyachenko OV; Buryanov YI [Ad] Endereço: Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia. shevchuk@bibch.ru. [Ti] Título: 35S Promoter Methylation in Kanamycin-Resistant Kalanchoe (Kalanchoe pinnata L.) Plants Expressing the Antimicrobial Peptide Cecropin P1 Transgene. [So] Source: Biochemistry (Mosc);81(9):968-71, 2016 Sep. [Is] ISSN: 1608-3040 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: Transgenic kalanchoe plants (Kalanchoe pinnata L.) expressing the antimicrobial peptide cecropin P1 gene (cecP1) under the control of the 35S cauliflower mosaic virus 35S RNA promoter and the selective neomycin phosphotransferase II (nptII) gene under the control of the nopaline synthase gene promoter were studied. The 35S promoter methylation and the cecropin P1 biosynthesis levels were compared in plants growing on media with and without kanamycin. The low level of active 35S promoter methylation further decreases upon cultivation on kanamycin-containing medium, while cecropin P1 synthesis increases. [Mh] Termos MeSH primário: Metilação de DNA DNA de Plantas Resistência a Medicamentos Proteínas de Insetos Kalanchoe Canamicina Plantas Geneticamente Modificadas Regiões Promotoras Genéticas Transgenes [Mh] Termos MeSH secundário: DNA de Plantas/genética DNA de Plantas/metabolismo Proteínas de Insetos/biossíntese Proteínas de Insetos/genética Kalanchoe/genética Kalanchoe/metabolismo Plantas Geneticamente Modificadas/genética Plantas Geneticamente Modificadas/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Plant); 0 (Insect Proteins); 0 (cecropin 1 protein, medfly); 59-01-8 (Kanamycin) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170113 [Lr] Data última revisão: 170113 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160930 [St] Status: MEDLINE [do] DOI: 10.1134/S0006297916090054

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[PMID]: 27472283 [Au] Autor: Ürményi FG; Saraiva GD; Casanova LM; Matos AD; de Magalhães Camargo LM; Romanos MT; Costa SS [Ad] Endereço: Laboratório de Química de Produtos Naturais Bioativos - LPN-Bio, Instituto de Pesquisas de Produtos Naturais, Av. Carlos Chagas Filho, 373 - Universidade Federal do Rio de Janeiro, Cidade Universitária, Rio de Janeiro, RJ, 21941-902, Brazil. [Ti] Título: Anti-HSV-1 and HSV-2 Flavonoids and a New Kaempferol Triglycoside from the Medicinal Plant Kalanchoe daigremontiana. [So] Source: Chem Biodivers;13(12):1707-1714, 2016 Dec. [Is] ISSN: 1612-1880 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: Kalanchoe daigremontiana (Crassulaceae) is a medicinal plant native to Madagascar. The aim of this study was to investigate the flavonoid content of an aqueous leaf extract from K. daigremontiana (Kd), and assess its antiherpetic potential. The major flavonoid, kaempferol 3-O-ß-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranoside (1), was isolated from the AcOEt fraction (Kd-AC). The BuOH-soluble fraction afforded quercetin 3-O-ß-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranoside (2) and the new kaempferol 3-O-ß-d-xylopyranosyl-(1 → 2)-α-l-rhamnopyranoside-7-O-ß-d-glucopyranoside (3), named daigremontrioside. The crude extract, Kd-AC fraction, flavonoids 1 and 2 were evaluated using acyclovir-sensitive strains of HSV-1 and HSV-2. Kd-AC was highly active against HSV-1 (EC  = 0.97 µg/ml, SI > 206.1) and HSV-2 (EC  = 0.72 µg/ml, SI > 277.7). Flavonoids 1 and 2 showed anti-HSV-1 (EC  = 7.4 µg/ml; SI > 27 and EC  = 5.8 µg/ml; SI > 8.6, respectively) and anti-HSV-2 (EC  = 9.0 µg/ml; SI > 22.2 and EC  = 36.2 µg/ml; SI > 5.5, respectively) activities, suggesting the contribution of additional substances to the antiviral activity. [Mh] Termos MeSH primário: Antivirais/farmacologia Glicosídeos/farmacologia Herpesvirus Humano 1/efeitos dos fármacos Herpesvirus Humano 2/efeitos dos fármacos Quempferóis/farmacologia Kalanchoe/química [Mh] Termos MeSH secundário: Antivirais/química Antivirais/isolamento & purificação Relação Dose-Resposta a Droga Glicosídeos/química Glicosídeos/isolamento & purificação Quempferóis/química Quempferóis/isolamento & purificação Testes de Sensibilidade Microbiana Estrutura Molecular Relação Estrutura-Atividade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antiviral Agents); 0 (Glycosides); 0 (Kaempferols) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170120 [Lr] Data última revisão: 170120 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160730 [St] Status: MEDLINE [do] DOI: 10.1002/cbdv.201600127

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[PMID]: 27393455 [Au] Autor: Manan M; Hussain L; Ijaz H; Qadir MI [Ad] Endereço: College of Pharmacy, Government College University, Faisalabad, Pakistan. [Ti] Título: Report: Antimicrobial activity of Kalanchoe laciniata. [So] Source: Pak J Pharm Sci;29(4):1321-4, 2016 Jul. [Is] ISSN: 1011-601X [Cp] País de publicação: Pakistan [La] Idioma: eng [Ab] Resumo: This study was conducted to identify antimicrobial potential of Kalanchoe laciniata. The plants were extracted with 30-70% aqueous-methanol and n-hexane. The antimicrobial activities were examined using agar well diffusion method against bacteria (Staphylococcus aureus, Escherichia coli) and fungi (Candidaalbicans). Results showed that E. coli were more sensitive than Staphylococcus aureus and Candida albicans. The largest zone of inhibition (52 mm) was recorded against E. coli with the n-hexane extract of Kalanchoe laciniata. [Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia Kalanchoe Extratos Vegetais/farmacologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Infective Agents); 0 (Plant Extracts) [Em] Mês de entrada: 1609 [Cu] Atualização por classe: 160709 [Lr] Data última revisão: 160709 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160710 [St] Status: MEDLINE

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[PMID]: 27220081 [Au] Autor: Fürer K; Simões-Wüst AP; von Mandach U; Hamburger M; Potterat O [Ad] Endereço: Division of Pharmaceutical Biology, Department of Pharmaceutical Sciences, University of Basel, Basel, Switzerland. [Ti] Título: Bryophyllum pinnatum and Related Species Used in Anthroposophic Medicine: Constituents, Pharmacological Activities, and Clinical Efficacy. [So] Source: Planta Med;82(11-12):930-41, 2016 Jul. [Is] ISSN: 1439-0221 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Bryophyllum pinnatum (syn. Kalanchoe pinnata) is a succulent perennial plant native to Madagascar that was introduced in anthroposophic medicine in the early 20th century. In recent years, we conducted a large collaborative project to provide reliable data on the chemical composition, pharmacological properties, and clinical efficacy of Bryophyllum. Here, we comprehensively review the phytochemistry, as well as the pharmacological and clinical data. As to the pharmacology, special emphasis is given to properties related to the use in anthroposophic medicine as a treatment for "hyperactivity diseases", such as preterm labor, restlessness, and sleep disorders. Studies suggesting that B. pinnatum may become a new treatment option for overactive bladder syndrome are also reviewed. Tolerability is addressed, and toxicological data are discussed in conjunction with the presence of potentially toxic bufadienolides in Bryophyllum species. The few data available on two related species with medicinal uses, Bryophyllum daigremontianum and Bryophyllum delagoense, have also been included. Taken together, current data support the use of B. pinnatum for the mentioned indications, but further studies are needed to fully understand the modes of action, and to identify the pharmacologically active constituents. [Mh] Termos MeSH primário: Kalanchoe/química Fitoterapia Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Animais Seres Humanos Extratos Vegetais/uso terapêutico Plantas Medicinais/química [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Plant Extracts) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170316 [Lr] Data última revisão: 170316 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160525 [St] Status: MEDLINE [do] DOI: 10.1055/s-0042-106727

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