Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.859.937.437.133 [Categoria DeCS]
Referências encontradas : 6 [refinar]
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  1 / 6 MEDLINE  
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[Au] Autor:Alvarez-Zapata R; Sánchez-Medina A; Chan-Bacab M; García-Sosa K; Escalante-Erosa F; García-Rodríguez RV; Peña-Rodríguez LM
[Ad] Endereço:Unidad de Biotecnología, Centro de Investigación Científica de Yucatán, Calle 43 N. 130, Col. Chuburná, CP 97200 Mérida, Yucatán, Mexico.
[Ti] Título:Chemometrics-enhanced high performance liquid chromatography-ultraviolet detection of bioactive metabolites from phytochemically unknown plants.
[So] Source:J Chromatogr A;1422:213-221, 2015 Nov 27.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:This work describes the use of Colubrina greggii as a model to investigate the use of chemometric analysis combined with data from a leishmanicidal bioassay, using Principal Component Analysis (PCA) and Orthogonal Projections to Latent Structures (O-PLS), to detect biologically active natural products in crude extracts from plants having little or no phytochemical information. A first analysis of the HPLC-UV profiles of the extract and its semi-purified fractions using both Principal Component Analysis (PCA) and Orthogonal Partial Least Squares (O-PLS) indicated that the components at tR 48.2, 48.7, 51.8min correlated with the variation in bioactivity. However, a further O-PLS analysis of the HPLC-UV profiles of fractions obtained through a final semi-preparative HPLC purification showed two components at tR 48.7 and 49.5min which correlated with the variation of the bioactivity in a high performance predictive model, with high determination coefficient, high correlation coefficient values (R(2) and Q(2)=0.99) and a low root mean square error (RMSE=0.018). This study demonstrates that the association of chemometric analysis with bioassay results can be an excellent strategy for the detection and isolation of bioactive metabolites from phytochemically unknown plant crude extracts.
[Mh] Termos MeSH primário: Compostos Fitoquímicos/metabolismo
Extratos Vegetais/metabolismo
[Mh] Termos MeSH secundário: Antiparasitários/isolamento & purificação
Cromatografia Líquida de Alta Pressão/métodos
Misturas Complexas
Análise dos Mínimos Quadrados
Leishmania mexicana/efeitos dos fármacos
Compostos Fitoquímicos/isolamento & purificação
Compostos Fitoquímicos/farmacologia
Plantas Medicinais
Análise de Componente Principal
Raios Ultravioleta
[Nm] Nome de substância:
0 (Antiparasitic Agents); 0 (Complex Mixtures); 0 (Phytochemicals); 0 (Plant Extracts)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:170930
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151031
[St] Status:MEDLINE

  2 / 6 MEDLINE  
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[Au] Autor:García MV; Prinz K; Barrandeguy ME; Miretti M; Finkeldey R
[Ad] Endereço:Facultad de Ciencias Exactas, Departamento de Genetica, Quimicas y Naturales, Universidad Nacional de Misiones, Posadas 3300, Argentina.
[Ti] Título:A unifying study of phenotypic and molecular genetic variability in natural populations of Anadenanthera colubrina var. cebil from Yungas and Paranaense biogeographic provinces in Argentina.
[So] Source:J Genet;93(1):123-32, 2014 Apr.
[Is] ISSN:0973-7731
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:Anadenanthera colubrina var. cebil is a discontinuously distributed native tree species in South American subtropical forests. Thirteen quantitative traits and eight nuclear microsatellite loci were examined in individuals from two biogeographic provinces of Argentina to determine the number and composition of genetically distinguishable groups of individuals and explore possible spatial patterns of the phenotypic and genetic variability. Means of reproductive traits were higher in the Yungas than in the Paranaense biogeographic province, whereas five out of eight nonreproductive quantitative traits showed higher mean values in the latter. Variance coefficients were moderate, and there were significant differences between and within provinces. Three clusters were defined based on spatial model for cluster membership for quantitative traits. One cluster grouped the individuals from the Paranaense biogeographic province whereas the individuals from the Yungas biogeographic province grouped regarding its population of origin. Parameters of molecular genetic variability showed higher values in the Yungas than in the Paranaense biogeographic province. Observed heterozygosity was lower than expected heterozygosity in both biogeographic provinces, indicating an excess of homozygosity. The homozygosity test by Watterson and the exact test by Slatkin suggested diversifying selection for locus Ac41.1. Bayesian clustering spatial model for microsatellites loci data were performed for both all loci and for all loci excluding locus Ac41.1. In both analyses two clusters were inferred. Analysis of molecular variance revealed similar results for all genotypes and for all genotypes defined excluding locus Ac41.1. Most of the total variance is attributable to genetic variation within clusters. The presence of homogeneous clusters was detected for both the phenotypic and molecular genetic variability. Two Bayesian clustering analyses were performed according to molecular genetic data, and two clusters were inferred. Individuals were assigned to their provinces of origin. Genetic molecular variation was higher in the populations of the Yungas biogeographic province which translates into highly qualified populations for conservation. Populations from the Paranaense biogeographic province showed the highest mean value of number of seeds per fruit making them valuable as well with regard to the exploitation of management strategies as a means to recover the impacted areas where these populations are located.
[Mh] Termos MeSH primário: Colubrina/genética
Estudos de Associação Genética
Variação Genética
Genética Populacional
[Mh] Termos MeSH secundário: Argentina
Análise por Conglomerados
Loci Gênicos
Repetições de Microssatélites
Característica Quantitativa Herdável
[Em] Mês de entrada:1504
[Cu] Atualização por classe:171108
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140521
[St] Status:MEDLINE

  3 / 6 MEDLINE  
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[Au] Autor:Damascena NP; Souza MT; Almeida AF; Cunha RS; Damascena NP; Curvello RL; Lima AC; Almeida EC; Santos CC; Dias AS; Paixão MS; Souza LM; Quintans Júnior LJ; Estevam CS; Araujo BS
[Ad] Endereço:a Department of Physiology , Federal University of Sergipe , São Cristovão , SE , Brazil.
[Ti] Título:Antioxidant and orofacial anti-nociceptive activities of the stem bark aqueous extract of Anadenanthera colubrina (Velloso) Brenan (Fabaceae).
[So] Source:Nat Prod Res;28(10):753-6, 2014.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The anti-nociceptive and antioxidant activities of the Anadenantheracolubrina stem bark aqueous extract (AEAC) were investigated. AEAC (30 µg/mL) reduced 94.8% of 2,2-diphenyl-1-picrylhydrazyl radical and prevented 64% (200 µg/mL) of lipid peroxidation caused by 2,2'-azobis(2-methylpropionamidine) dihydrochloride-induced peroxyl radicals. AEAC treatment (200 and 400 mg/kg) significantly (p < 0.001) reduced mice orofacial nociception in the first (61.4% and 62.6%, respectively) and second (48.9% and 61.9%, respectively) phases of the formalin test. Nociception caused by glutamate was significantly (p < 0.001) reduced by up to 79% at 400 mg/kg, while 56-60% of the nociceptive behaviour induced by capsaicin was significantly inhibited by AEAC (100-400 mg/kg). Mice treated with AEAC did not show changes in motor performance in the Rota-rod apparatus. It appears that AEAC is of pharmacological importance in treating pain due to its anti-nociceptive effects, which were shown to be mediated by central and peripheral mechanisms.
[Mh] Termos MeSH primário: Analgésicos/farmacologia
[Mh] Termos MeSH secundário: Amidinas/farmacologia
Analgésicos/uso terapêutico
Antioxidantes/uso terapêutico
Compostos de Bifenilo/farmacologia
Ácido Glutâmico/farmacologia
Peroxidação de Lipídeos
Dor/tratamento farmacológico
Medição da Dor
Casca de Planta/química
Extratos Vegetais/farmacologia
Caules de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amidines); 0 (Analgesics); 0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Picrates); 0 (Plant Extracts); 3KX376GY7L (Glutamic Acid); 7381JDR72F (2,2'-azobis(2-amidinopropane)); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl); S07O44R1ZM (Capsaicin)
[Em] Mês de entrada:1410
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140121
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2013.877902

  4 / 6 MEDLINE  
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Queiroz, Mary Luci de Souza
Texto completo
[Au] Autor:Pereda Mdel C; Dieamant Gde C; Eberlin S; Werka RM; Colombi D; Queiroz ML; Di Stasi LC
[Ad] Endereço:Department of Pharmacology/Hemocenter, University of Campinas (UNICAMP), Campinas, Brazil.
[Ti] Título:Expression of differential genes involved in the maintenance of water balance in human skin by Piptadenia colubrina extract.
[So] Source:J Cosmet Dermatol;9(1):35-43, 2010 Mar.
[Is] ISSN:1473-2165
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Hydration and integrity of the stratum corneum (SC) is an important determinant of skin appearance, metabolism, mechanical properties, and barrier function. The presence of aquaglyceroporins and envelope proteins are crucial to provide greater corneocyte cohesion to keep water and other moisturizers in the skin. AIMS: In this study, we evaluated the ability of Piptadenia colubrina, a plant native of South American rain forests, in the expression of genes involved in skin capacitance and SC integrity. METHODS: The expression of genes for aquaporin-3 (AQP3), loricrin, involucrin (INV), and filaggrin (FLG) was measured by real-time PCR, using an in vitro model of human keratinocytes incubated with concentrations of 2.5, 5, 10, and 20 mg/mL of a hydroglycolic extract of P. colubrina (HEPC). The amount of AQP3 protein was also tested by immunohistochemistry in human skin explants. Clinical trials were conducted to evaluate the effects of a gel-cream containing HEPC on the glycerol index and skin capacitance. RESULTS: Hydroglycolic extract of P. colubrina increased both the expression and immunoreactivity of AQP3 in cultured keratinocytes and human skin explants. The gene induction to envelope proteins FLG and INV was also observed after cell incubation with HEPC. Skin capacitance was significantly improved in human volunteers under treatment with HEPC-containing cream. CONCLUSIONS: The extract of P. colubrina promotes cellular hydration and induces gene expression of envelope proteins providing greater corneocyte cohesion to keep water and other moisturizers in the skin and an appropriate epidermal adhesion. The in vitro findings were clinically confirmed and encourage the clinical use of this compound in skin care products.
[Mh] Termos MeSH primário: Aquaporina 3/metabolismo
Proteínas de Filamentos Intermediários/metabolismo
Proteínas de Membrana/metabolismo
Precursores de Proteínas/metabolismo
Pele/efeitos dos fármacos
[Mh] Termos MeSH secundário: Administração Cutânea
Aquaporina 3/genética
Emolientes/administração & dosagem
Seres Humanos
Técnicas In Vitro
Proteínas de Filamentos Intermediários/genética
Queratinócitos/efeitos dos fármacos
Proteínas de Membrana/genética
Extratos Vegetais/administração & dosagem
Extratos Vegetais/farmacologia
Reação em Cadeia da Polimerase
Precursores de Proteínas/genética
Absorção Cutânea/efeitos dos fármacos
Equilíbrio Hidroeletrolítico
[Nm] Nome de substância:
0 (Emollients); 0 (Intermediate Filament Proteins); 0 (Membrane Proteins); 0 (Plant Extracts); 0 (Protein Precursors); 0 (filaggrin); 0 (loricrin); 059QF0KO0R (Water); 158801-98-0 (Aquaporin 3); 60108-77-2 (involucrin)
[Em] Mês de entrada:1007
[Cu] Atualização por classe:141120
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100407
[St] Status:MEDLINE
[do] DOI:10.1111/j.1473-2165.2009.00458.x

  5 / 6 MEDLINE  
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[Au] Autor:Alleyne T; Roache S; Thomas C; Shirley A
[Ad] Endereço:Department of Preclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St Augustine, Trinidad and Tobago, West Indies.
[Ti] Título:The control of hypertension by use of coconut water and mauby: two tropical food drinks.
[So] Source:West Indian Med J;54(1):3-8, 2005 Jan.
[Is] ISSN:0043-3144
[Cp] País de publicação:Jamaica
[La] Idioma:eng
[Ab] Resumo:In this study, the authors investigated the effect of regular consumption of two tropical food drinks, coconut (Cocos nucifera) water and mauby (Colubrina arborescens), on the control of hypertension. Twenty-eight hypertensive subjects were assigned to four equal groups and their systolic and diastolic blood pressures recorded for two weeks before and then for another two weeks while receiving one of four interventions. One group (the control) received bottled drinking water, the second group received coconut water, the third received mauby and the fourth group, a mixture of coconut water and mauby. Significant decreases in the mean systolic blood pressure were observed for 71%, 40% and 43% respectively of the groups receiving the coconut water, mauby and the mixture (p < or = 0.05). For these groups, the respective proportions showing significant decreases in the mean diastolic pressure were 29%, 40% and 57%. For the group receiving the mixture, the largest decreases in mean systolic and mean diastolic pressure were 24 mmHg and 15 mmHg respectively; these were approximately double the largest values seen with the single interventions.
[Mh] Termos MeSH primário: Bebidas
Hipertensão/prevenção & controle
Preparações de Plantas/uso terapêutico
[Mh] Termos MeSH secundário: Pressão Sanguínea/fisiologia
Estudos de Casos e Controles
Seres Humanos
Método Simples-Cego
Trinidad e Tobago
[Nm] Nome de substância:
0 (Plant Preparations); 059QF0KO0R (Water)
[Em] Mês de entrada:0506
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:050517
[St] Status:MEDLINE

  6 / 6 MEDLINE  
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[Au] Autor:Kwon JA; Morden CW
[Ad] Endereço:Department of Botany, 3190 Maile Way, University of Hawai'i, Honolulu 96822, USA.
[Ti] Título:Population genetic structure of two rare tree species (Colubrina oppositifolia and Alphitonia ponderosa, Rhamnaceae) from Hawaiian dry and mesic forests using random amplified polymorphic DNA markers.
[So] Source:Mol Ecol;11(6):991-1001, 2002 Jun.
[Is] ISSN:0962-1083
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Hawaiian dry and mesic forests contain an increasingly rare assemblage of species due to habitat destruction, invasive alien weeds and exotic pests. Two rare Rhamnaceae species in these ecosystems, Colubrina oppositifolia and Alphitonia ponderosa, were examined using random amplified polymorphic DNA (RAPD) markers to determine the genetic structure of the populations and the amount of variation relative to other native Hawaiian species. Relative variation is lower than with other Hawaiian species, although this is probably not a consequence of genetic bottleneck. Larger populations of both species contain the highest levels of genetic diversity and smaller populations generally the least as determined by number of polymorphic loci, estimated heterozygosity, and Shannon's index of genetic diversity. Populations on separate islands were readily discernible for both species as were two populations of C. oppositifolia on Hawai'i island (North and South Kona populations). Substructure among Kaua'i subpopulations of A. ponderosa that were ecologically separated was also evident. Although population diversity is thought to have remained at predisturbance levels, population size continues to decline as recruitment is either absent or does not keep pace with senescence of mature plants. Recovery efforts must focus on control of alien species if these and other endemic dry and mesic forest species are to persist.
[Mh] Termos MeSH primário: Colubrina/genética
[Mh] Termos MeSH secundário: Colubrina/classificação
Primers do DNA/genética
Genes de Plantas
Genética Populacional
Técnica de Amplificação ao Acaso de DNA Polimórfico
[Nm] Nome de substância:
0 (DNA Primers)
[Em] Mês de entrada:0302
[Cu] Atualização por classe:061115
[Lr] Data última revisão:
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:020529
[St] Status:MEDLINE

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