Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.859.937.500.266 [Categoria DeCS]
Referências encontradas : 1324 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 133 ir para página                         

  1 / 1324 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29458464
[Au] Autor:Melo LA; Ventura JA; Costa H; Kitajima EW; Ferreira J; Bedendo IP
[Ad] Endereço:1​Departamento de Fitopatologia e Nematologia, ESALQ, Universidade de São Paulo, Caixa Postal 09, 13418-900 Piracicaba, SP, Brazil.
[Ti] Título:Delineation of a novel subgroup 16SrXIII-J phytoplasma, a 'Candidatus Phytoplasma hispanicum'-related strain, based on computer-simulated RFLP and phylogenetic analysis.
[So] Source:Int J Syst Evol Microbiol;68(3):962-966, 2018 Mar.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Symptoms of fruit phyllody and slow growth, which are suggestive of phytoplasma infection, were observed in strawberry plants cultivated in commercial fields. In order to provide evidence of association of phytoplasma with affected plants, assays for detecting and identifying were performed through computer-simulated restriction fragment length polymorphism (RFLP) and phylogenetic analysis. Total DNA was extracted from symptomatic and asymptomatic samples and used as template in nested PCR primed by the primers P1/Tint followed by R16F2n/16R2. Amplified DNA fragments of 1.2 kb from the 16S rRNA gene revealed the presence of phytoplasma in all symptomatic samples. Molecular detection was confirmed by electron transmission microscopy, which evidenced pleomorphic bodies in the phloem vessels. Nucleotide sequence representative of the strawberry phytoplasma shared 97.2 to 99 % similarity with phytoplasmas currently classified as members of the distinct subgroups within the 16SrXIII group. Similarity coefficient (F) values ranged from 0.70 to 0.92, indicating that strawberry phytoplasma delineates a new strain in addition to 'Candidatus Phytoplasma hispanicum'-related strains. The evolutionary tree displayed that this strain emerges as a new branch in relation to those previously described. The novel strain, designated SFP (strawberry fruit phyllody) phytoplasma represents the new 16SrXIII-J subgroup and its sequence, denominated SFP-Br02, was deposited in the GenBank database (EU719108). These findings contribute for the knowledge of the genetic diversity existing among members of the group 16SrXIII and establishes strawberry as an additional host of representatives of this group in Brazil.
[Mh] Termos MeSH primário: Fragaria/microbiologia
Filogenia
Phytoplasma/classificação
Doenças das Plantas/microbiologia
Polimorfismo de Fragmento de Restrição
[Mh] Termos MeSH secundário: Técnicas de Tipagem Bacteriana
Brasil
Primers do DNA
DNA Bacteriano/genética
Reação em Cadeia da Polimerase
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180308
[Lr] Data última revisão:
180308
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180221
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002547


  2 / 1324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29179062
[Au] Autor:Xu Y; Hu D; Li Y; Sun C; Chen W
[Ad] Endereço:Department of Food Science and Nutrition, National Engineering Laboratory of Intelligent Food Technology and Equipment, Key Laboratory for Agro-Products Postharvest Handling of Ministry of Agriculture, Zhejiang Key Laboratory for Agro-Food Processing, Fuli Institute of Food Science, Zhejiang Univers
[Ti] Título:An effective method for preparation of high-purity pelargonidin-3-O-glucoside from strawberry and its protective effect on cellular oxidative stress.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1072:211-220, 2018 Jan 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Accumulating evidence indicates that consumption of berries may exert beneficial effects against oxidative stress mediated diseases. Pelargonidin-3-O-glucoside (Pg3G), a bioactive ingredient in strawberry, has been reported to possess a potent antioxidant capacity. This study was therefore designed to develop an effective method to prepare pure Pg3G from strawberry and investigate its protective effect against H O -induced oxidative stress. According to our results, Pg3G occupied 85.55% of total anthocyanin content in strawberry. 240mg of Pg3G with the purity of 97.26% was finally isolated from 320g of strawberry lyophilized powder (SLP) by combination of AB-8 macroporous resin and high-speed counter-current chromatography (HSCCC) technologies. Further study unveiled that Pg3G significantly inhibited H O -induced ROS generation, GSH depletion and mitochondrial dysfunction, thereby ameliorating H O -induced oxidative stress. Overall, this study suggests that pelargonidin-3-O-glucoside can be used as a natural bioactive agent to prevent cellular oxidative stress.
[Mh] Termos MeSH primário: Antocianinas/isolamento & purificação
Antocianinas/farmacologia
Antioxidantes/isolamento & purificação
Antioxidantes/farmacologia
Fragaria/química
Estresse Oxidativo/efeitos dos fármacos
[Mh] Termos MeSH secundário: Antocianinas/análise
Antocianinas/química
Antioxidantes/análise
Antioxidantes/química
Sobrevivência Celular/efeitos dos fármacos
Distribuição Contracorrente/métodos
Glutationa/metabolismo
Células Hep G2
Seres Humanos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Antioxidants); 8H1WZY9R6P (pelargonidin-3-glucoside); GAN16C9B8O (Glutathione)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180221
[Lr] Data última revisão:
180221
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171128
[St] Status:MEDLINE


  3 / 1324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29297687
[Au] Autor:Jezek M; Zörb C; Merkt N; Geilfus CM
[Ad] Endereço:Laboratory of Plant Physiology and Biophysics, University of Glasgow , Glasgow G12 8QQ, United Kingdom.
[Ti] Título:Anthocyanin Management in Fruits by Fertilization.
[So] Source:J Agric Food Chem;66(4):753-764, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Anthocyanins are water-soluble vacuolar plant pigments that are mainly synthesized in epidermal layers and the flesh of fruits such as apples, cherries, grapes, and other berries. Because of their attractive red to purple coloration and their health-promoting potential, anthocyanins are significant determinants for the quality and market value of fruits and fruit-derived products. In crops, anthocyanin accumulation in leaves can be caused by nutrient deficiency which is usually ascribed to insufficient nitrogen or phosphorus fertilization. However, it is a little-known fact that the plant's nutrient status also impacts anthocyanin synthesis in fruits. Hence, strategic nutrient supply can be a powerful tool to modify the anthocyanin content and consequently the quality and market value of important agricultural commodities. Here we summarize the current knowledge of the influence of plant nutrients on anthocyanin synthesis in fruits of major global market value and discuss the underlying cellular processes that integrate nutrient signaling with fruit anthocyanin formation. It is highlighted that fertilization that is finely tuned in amount and timing has the potential to positively influence the fruit quality by regulating anthocyanin levels. We outline new approaches to enrich plant based foods with health-promoting anthocyanins.
[Mh] Termos MeSH primário: Agricultura/métodos
Antocianinas/biossíntese
Produtos Agrícolas/crescimento & desenvolvimento
Fertilizantes
Frutas/metabolismo
[Mh] Termos MeSH secundário: Antocianinas/análise
Produtos Agrícolas/fisiologia
Fragaria/química
Frutas/química
Frutas/crescimento & desenvolvimento
Promoção da Saúde
Seres Humanos
Malus/química
Folhas de Planta/metabolismo
Vitis/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Fertilizers)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180104
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b03813


  4 / 1324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29291263
[Au] Autor:Giampieri F; Gasparrini M; Forbes-Hernandez TY; Mazzoni L; Capocasa F; Sabbadini S; Alvarez-Suarez JM; Afrin S; Rosati C; Pandolfini T; Molesini B; Sánchez-Sevilla JF; Amaya I; Mezzetti B; Battino M
[Ti] Título:Overexpression of the Anthocyanidin Synthase Gene in Strawberry Enhances Antioxidant Capacity and Cytotoxic Effects on Human Hepatic Cancer Cells.
[So] Source:J Agric Food Chem;66(3):581-592, 2018 Jan 24.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Food fortification through the increase and/or modulation of bioactive compounds has become a major goal for preventing several diseases, including cancer. Here, strawberry lines of cv. Calypso transformed with a construct containing an anthocyanidin synthase (ANS) gene were produced to study the effects on anthocyanin biosynthesis, metabolism, and transcriptome. Three strawberry ANS transgenic lines (ANS L5, ANS L15, and ANS L18) were analyzed for phytochemical composition and total antioxidant capacity (TAC), and their fruit extracts were assessed for cytotoxic effects on hepatocellular carcinoma. ANS L18 fruits had the highest levels of total phenolics and flavonoids, while those of ANS L15 had the highest anthocyanin concentration; TAC positively correlated with total polyphenol content. Fruit transcriptome was also specifically affected in the polyphenol biosynthesis and in other related metabolic pathways. Fruit extracts of all lines exerted cytotoxic effects in a dose/time-dependent manner, increasing cellular apoptosis and free radical levels and impairing mitochondrial functionality.
[Mh] Termos MeSH primário: Antioxidantes/análise
Fragaria/enzimologia
Frutas/química
Neoplasias Hepáticas/tratamento farmacológico
Oxigenases/genética
Proteínas de Plantas/genética
[Mh] Termos MeSH secundário: Antocianinas/análise
Antocianinas/biossíntese
Antocianinas/farmacologia
Antioxidantes/metabolismo
Antioxidantes/farmacologia
Apoptose
Fragaria/química
Fragaria/genética
Frutas/enzimologia
Frutas/genética
Células Hep G2
Seres Humanos
Neoplasias Hepáticas/genética
Neoplasias Hepáticas/metabolismo
Neoplasias Hepáticas/fisiopatologia
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Oxigenases/metabolismo
Proteínas de Plantas/metabolismo
Polifenóis/análise
Polifenóis/metabolismo
Polifenóis/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Antioxidants); 0 (Plant Proteins); 0 (Polyphenols); EC 1.13.- (Oxygenases); EC 1.14.99.- (anthocyanidin synthase)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180207
[Lr] Data última revisão:
180207
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180102
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b04177


  5 / 1324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:27775146
[Au] Autor:Dias MI; Barros L; Morales P; Cámara M; Alves MJ; Oliveira MB; Santos-Buelga C; Ferreira IC
[Ad] Endereço:Mountain Research Centre (CIMO), ESA, Polytechnic Institute of Bragança, Campus de Santa Apolónia, 1172, 5300-253 Bragança, Portugal. iferreira@ipb.pt and REQUIMTE/LAQV, Science Chemical Department, Faculty of Pharmacy of University of Porto, Rua Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal
[Ti] Título:Wild Fragaria vesca L. fruits: a rich source of bioactive phytochemicals.
[So] Source:Food Funct;7(11):4523-4532, 2016 Nov 09.
[Is] ISSN:2042-650X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Wild Fragaria vesca L. fruits were studied regarding nutritional and phytochemical compounds, and also antioxidant, antibacterial and biofilm formation inhibition activities. The fruits are good sources of carbohydrates (e.g., sucrose), soluble dietary fiber and polyunsaturated fatty acids, mainly linoleic and linolenic acids, as well as other components such as citric and succinic acids, and vitamins B and E (mainly γ-tocopherol). Significant amounts of soluble sugars, citric acid and some amounts of ascorbic acid, vitamins B and E (only α-tocopherol) were found also in the infusions. The hydromethanolic extracts revealed higher amounts of phenolic compounds, mainly ellagic acid derivatives and dihydroflavonol taxifolin-3-O-arabinofuranoside. Consistently, these extracts also showed higher antioxidant and antibacterial activities than the infusions, and were able to inhibit the formation of bacterial biofilms. Despite the lower content of bioactive compounds in the infusions compared to the fruits, both forms could be potentially applied in functional foods and/or nutraceuticals/pharmaceutical formulations.
[Mh] Termos MeSH primário: Fragaria/química
Frutas/química
Compostos Fitoquímicos/química
Compostos Fitoquímicos/farmacologia
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: Antibacterianos/química
Antibacterianos/farmacologia
Antioxidantes/química
Antioxidantes/farmacologia
Bactérias/efeitos dos fármacos
Valor Nutritivo
Fenóis/química
Fenóis/farmacologia
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antioxidants); 0 (Phenols); 0 (Phytochemicals); 0 (Plant Extracts)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180201
[Lr] Data última revisão:
180201
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161025
[St] Status:MEDLINE


  6 / 1324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28585314
[Au] Autor:Adak N; Gubbuk H; Tetik N
[Ad] Endereço:Akdeniz University, Vocational Higher School of Technical Sciences, Antalya, Turkey.
[Ti] Título:Yield, quality and biochemical properties of various strawberry cultivars under water stress.
[So] Source:J Sci Food Agric;98(1):304-311, 2018 Jan.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Although strawberry (Fragaria x ananassa Duch.) species are sensitive to abiotic stress conditions, some cultivars are known to be tolerant to different environmental conditions. We examined the response of different strawberry cultivars to water stress conditions in terms of yield, quality and biochemical features. The trial was conducted under two different irrigation regimes: in grow bags containing cocopeat (control, 30%; water stress, 15% drainage) with four different cultivars (Camarosa, Albion, Amiga and Rubygem). RESULTS: Fruit weight declined by 59.72% and the yield per unit area by 63.62% under water stress conditions as compared to control. Albion and Rubygem were found to be more tolerant and Amiga the most sensitive in terms of yield under stress conditions. Water stress increased all biochemical features in fruits such as total phenol, total anthocyanin, antioxidant activity and sugar contents. Among the cultivars, glucose and fructose was higher in Albion. CONCLUSION: Considering the rise in global warming, identification of resistant and tolerant cultivars to stress conditions are crucial for future breeding programmes. Our results showed that some of the fruit's physical features were affected negatively by stress conditions whereas many of the biochemical features such as total anthocyanin content, total phenolic content and antioxidant activity were positively modulated. © 2017 Society of Chemical Industry.
[Mh] Termos MeSH primário: Fragaria/química
Fenóis/análise
Extratos Vegetais/análise
Água/análise
[Mh] Termos MeSH secundário: Antocianinas/análise
Antocianinas/metabolismo
Antioxidantes/análise
Antioxidantes/metabolismo
Fragaria/metabolismo
Frutas/química
Frutas/crescimento & desenvolvimento
Frutas/metabolismo
Valor Nutritivo
Fenóis/metabolismo
Controle de Qualidade
Água/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Antioxidants); 0 (Phenols); 0 (Plant Extracts); 059QF0KO0R (Water)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171228
[Lr] Data última revisão:
171228
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170607
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.8471


  7 / 1324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28873625
[Au] Autor:Ribes S; Fuentes A; Talens P; Barat JM
[Ad] Endereço:Food Technology Department, Universitat Politècnica de València, Camino de Vera s/n, 46022 Valencia, Spain. Electronic address: surillo@upv.es.
[Ti] Título:Combination of different antifungal agents in oil-in-water emulsions to control strawberry jam spoilage.
[So] Source:Food Chem;239:704-711, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The combination of antifungal agents (cinnamon bark oil, zinc gluconate and trans-ferulic acid) in oil-in-water emulsions to control the fungal spoilage of strawberry jams, minimising essential oil's sensory impact, was evaluated in this work. The in vitro assays of free antifungal agents were performed against five fungal strains; meanwhile, the emulsions assays were conducted against Aspergillus niger given its strong resistance and its relevance in strawberry products. The emulsion formulated with 0.08mg/g of essential oil was able to inhibit mould growth after the incubation period. The incorporation of zinc gluconate or trans-ferulic acid, independently of the concentration used, allowed to reduce a 25% the amount of essential oil needed to inhibit the microbial growth. The combination of antifungal agents in the emulsions has demonstrated to be an effective alternative to reduce the amount of essential oil employed, maintaining the hygienic quality and sensory profile of the strawberry jam.
[Mh] Termos MeSH primário: Fragaria
[Mh] Termos MeSH secundário: Antifúngicos
Aspergillus niger
Emulsões
Alimentos em Conserva
Óleos Voláteis
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Emulsions); 0 (Oils, Volatile)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  8 / 1324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28873594
[Au] Autor:Perini M; Giongo L; Grisenti M; Bontempo L; Camin F
[Ad] Endereço:Experiment and Technological Services Department, Technology Transfer Centre, Fondazione Edmund Mach (FEM), Via E. Mach 1, 38010 San Michele all'Adige, Italy. Electronic address: matteo.perini@fmach.it.
[Ti] Título:Stable isotope ratio analysis of different European raspberries, blackberries, blueberries, currants and strawberries.
[So] Source:Food Chem;239:48-55, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:To date the stable isotope ratios of berries have never been extensively explored. In this work the H, C, N and O isotopic ratios of 190 samples of different soft fruits (strawberries, raspberries, blueberries, blackberries and currants) produced in a northern Italian region and at two sites in Romania and Poland collected over three harvest years are presented and discussed. The different soft fruits showed a typical range for one or more isotopic parameters that can be used to verify the authenticity of the fruit composition declared on the label. The δ C and δ N of pulp and the δ O of juice can be considered effective tools for identifying the different geographical origin of fruit. A significant effect of crop cover on juice δ O and fertilisation practices on pulp δ N was demonstrated and must be considered with attention when evaluating data.
[Mh] Termos MeSH primário: Fragaria
Ribes
Rubus
[Mh] Termos MeSH secundário: Isótopos
Polônia
Romênia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Isotopes)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


  9 / 1324 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28448788
[Au] Autor:Arhoun B; Gomez-Lahoz C; Abdala-Diaz RT; Rodriguez-Maroto JM; Garcia-Herruzo F; Vereda-Alonso C
[Ad] Endereço:a Chemical Engineering Department, Faculty of Sciences , University of Malaga , Malaga , Spain.
[Ti] Título:Production of biogas from co-digestion of livestock and agricultural residues: A case study.
[So] Source:J Environ Sci Health A Tox Hazard Subst Environ Eng;52(9):856-861, 2017 Jul 29.
[Is] ISSN:1532-4117
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study was undertaken to determine the possible changes in the digester yield and performance for the anaerobic co-digestion under mesophilic conditions of strawberry residues (SRs) together with pig manure (PM). The first part of this paper deals with the digestion of SR as a single substrate. For organic loading rates (OLRs) of 4.4 (g L d ) or less, the experimental specific biogas and methane productions are 0.588 and 0.231 L g , respectively. When higher OLRs (5.5 g L d ) are used the digester fails due to acidification. In the second part, the co-digestion of both residues is explored using a wide variety of SR:PM ratios and OLRs of 5.5 g L d with good stability. Therefore, it is demonstrated that co-digestion allows the improvement of the treatment capacity as compared with SR as a single residue. The methane and biogas productions increase as the SR:PM ratio increases. It may be concluded that, when a digester works with a certain OLR, the performance for co-digestion is always better than for single substrates because the presence of PM provides a better stability and the presence of SR improves the biogas and methane production.
[Mh] Termos MeSH primário: Biocombustíveis/análise
Reatores Biológicos
Biotecnologia/métodos
Fragaria/química
Esterco/análise
Metano/biossíntese
[Mh] Termos MeSH secundário: Anaerobiose
Animais
Reatores Biológicos/microbiologia
Biotecnologia/instrumentação
Sus scrofa
Resíduos/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 0 (Manure); 0 (Waste Products); OP0UW79H66 (Methane)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170428
[St] Status:MEDLINE
[do] DOI:10.1080/10934529.2017.1312189


  10 / 1324 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28985484
[Au] Autor:Abate-Pella D; Freund DM; Slovin JP; Hegeman AD; Cohen JD
[Ad] Endereço:Department of Horticultural Science, University of Minnesota, Saint Paul, MN, USA; Microbial and Plant Genomics Institute, University of Minnesota, Saint Paul, MN, USA.
[Ti] Título:An improved method for fast and selective separation of carotenoids by LC-MS.
[So] Source:J Chromatogr B Analyt Technol Biomed Life Sci;1067:34-37, 2017 Nov 01.
[Is] ISSN:1873-376X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Carotenoids are a large class of compounds that are biosynthesized by condensation of isoprene units in plants, fungi, bacteria, and some animals. They are characteristically highly conjugated through double bonds, which lead to many isomers as well susceptibility to oxidation and other chemical modifications. Carotenoids are important because of their potent antioxidant activity and are the pigments responsible for color in a wide variety of foods. Human consumption is correlated to many health benefits including prevention of cancer, cardiovascular disease, and age-related disease. Extreme hydrophobicity, poor stability, and low concentration in biological samples make these compounds difficult to analyze and difficult to develop analytical methods for aimed towards identification and quantification. Examples in the literature frequently report the use of exotic stationary phases, solvents, and additives, such as ethyl acetate, dichloromethane, and methyl tert-butyl ether that are incompatible with liquid chromatography mass spectrometry (LC-MS). In order to address these issues, we implemented the use of LC-MS friendly conditions using a low-hydrophobicity cyano-propyl column (Agilent Zorbax SB-CN). We successfully differentiated between isomeric carotenoids by optimizing two gradient methods and using a mixture of 11 standards and LC-MS in positive ionization mode. Three complex biological samples from strawberry leaf, chicken feed supplement, and the photosynthetic bacterium Chloroflexus aurantiacus were analyzed and several carotenoids were resolved in these diverse backgrounds. Our results show this methodology is a significant improvement over other alternatives for analyzing carotenoids because of its ease of use, rapid analysis time, high selectivity, and, most importantly, its compatibility with typical LC-MS conditions.
[Mh] Termos MeSH primário: Carotenoides/análise
Carotenoides/isolamento & purificação
Cromatografia Líquida/métodos
Espectrometria de Massas/métodos
[Mh] Termos MeSH secundário: Ração Animal/análise
Carotenoides/química
Chloroflexus/química
Fragaria/química
Isomerismo
Modelos Químicos
Folhas de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
36-88-4 (Carotenoids)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171024
[Lr] Data última revisão:
171024
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171007
[St] Status:MEDLINE



página 1 de 133 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde