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[PMID]:28829780
[Au] Autor:Guo Y; Wang Z; Guan X; Hu Z; Zhang Z; Zheng J; Lu Y
[Ad] Endereço:College of Landscape Architecture, Beijing University of Agriculture, Beijing, China.
[Ti] Título:Proteomic analysis of Potentilla fruticosa L. leaves by iTRAQ reveals responses to heat stress.
[So] Source:PLoS One;12(8):e0182917, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:High temperature is an important environmental factor that affects plant growth and crop yield. Potentilla fruticosa L. has a developed root system and characteristics of resistance to several stresses (e.g., high temperature, cold, drought) that are shared by native shrubs in the north and west of China. To investigate thermotolerance mechanisms in P. fruticosa, 3-year-old plants were subjected to a high temperature of 42°C for 1, 2, and 3 days respectively before analysis. Then, we studied changes in cell ultrastructure using electron microscopy and investigated physiological changes in the leaves of P. fruticosa. Additionally, we used isobaric tags for relative and absolute quantification (iTRAQ) coupled with liquid chromatography-tandem mass spectrometry (LC-MS/MS) to study proteomic changes in P. fruticosa leaves after 3 d of 42°C heat stress. we found that the cell membrane and structure of chloroplasts, especially the thylakoids in P. fruticosa leaves, was destroyed by a high temperature stress, which might affect the photosynthesis in this species. We identified 35 up-regulated and 23 down-regulated proteins after the heat treatment. Gene Ontology (GO) analysis indicated that these 58 differentially abundant proteins were involved mainly in protein synthesis, protein folding and degradation, abiotic stress defense, photosynthesis, RNA process, signal transduction, and other functions. The 58 proteins fell into different categories based on their subcellular localization mainly in the chloroplast envelope, cytoplasm, nucleus, cytosol, chloroplast, mitochondrion and cell membrane. Five proteins were selected for analysis at the mRNA level; this analysis showed that gene transcription levels were not completely consistent with protein abundance. These results provide valuable information for Potentilla thermotolerance breeding.
[Mh] Termos MeSH primário: Folhas de Planta/metabolismo
Potentilla/metabolismo
Proteômica
Estresse Fisiológico
[Mh] Termos MeSH secundário: Cromatografia Líquida
Temperatura Alta
Espectrometria de Massas em Tandem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170823
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0182917


  2 / 164 MEDLINE  
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[PMID]:28603128
[Au] Autor:Li S; Tan J; Zeng J; Xianjin Wu XW; Zhang J
[Ad] Endereço:Department of Life Science, Huaihua University, Huaihua, China / Key Laboratory of Hunan Province for Study and Utilization of Ethnic Medicinal Plant Resources, Huaihua, China / Key Laboratory of Hunan Higher Education for Hunan-Western Medicinal Plant and Ethnobotany, Huaihua, China.
[Ti] Título:Antihyperglycemic and antioxidant effect of the total flavones of Potentilla kleiniana Wight et Arn. in streptozotocin induced diabetic rats.
[So] Source:Pak J Pharm Sci;30(1):171-178, 2017 Jan.
[Is] ISSN:1011-601X
[Cp] País de publicação:Pakistan
[La] Idioma:eng
[Ab] Resumo:To investigate the antihyperglycemic and antioxidant activity of the total flavones of Potentilla kleiniana Wight et Arn. (TFP) in streptozotocin (STZ) induced diabetic rats. STZ-induced diabetic rats were treated with TFP weekly for 4 weeks at three doses (100 mg/kg, 200 mg/kg and 400 mg/kg). Blood glucose levels (BGL), body weight, insulin, total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), very low density lipoprotein (VLDL), low density lipoprotein (LDL), malondialdehyde (MDA), glutathione (GSH), super oxide dismutase (SOD) and catalase (CAT) levels of liver and pancreas were measured weekly for 4 weeks. STZ administration resulted in oxidative damage of pancreas, then hyperglycemia proved by higher MDA, lower insulin and higher BGL in comparison to normal rats. TC, TG, LDL and VLDL cholesterol levels were also significantly elevated with decreased GSH, SOD, and CAT levels. A steady decrease in BGL and increase in insulin level were observed 4 weeks after TFP treatment in a dose dependent manner, as well as remarkable improvement in body weight and biochemical parameters. TFP have the effect of inhibiting hyperglycemia and oxidative stress, and the administration may be helpful in the prevention of diabetic complications associated with oxidative stress.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Glicemia/efeitos dos fármacos
Diabetes Mellitus Experimental/tratamento farmacológico
Flavonas/farmacologia
Hipoglicemiantes/farmacologia
Estresse Oxidativo/efeitos dos fármacos
Extratos Vegetais/farmacologia
Potentilla/química
Estreptozocina
[Mh] Termos MeSH secundário: Animais
Antioxidantes/isolamento & purificação
Biomarcadores/sangue
Glicemia/metabolismo
Diabetes Mellitus Experimental/sangue
Diabetes Mellitus Experimental/induzido quimicamente
Diabetes Mellitus Experimental/patologia
Relação Dose-Resposta a Droga
Flavonas/isolamento & purificação
Hipoglicemiantes/isolamento & purificação
Lipídeos/sangue
Fígado/efeitos dos fármacos
Fígado/metabolismo
Fígado/patologia
Masculino
Pâncreas/efeitos dos fármacos
Pâncreas/metabolismo
Pâncreas/patologia
Fitoterapia
Extratos Vegetais/isolamento & purificação
Plantas Medicinais
Ratos Wistar
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biomarkers); 0 (Blood Glucose); 0 (Flavones); 0 (Hypoglycemic Agents); 0 (Lipids); 0 (Plant Extracts); 5W494URQ81 (Streptozocin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171103
[Lr] Data última revisão:
171103
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE


  3 / 164 MEDLINE  
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[PMID]:28388692
[Au] Autor:Zhang J; Liu C; Huang RZ; Chen HF; Liao ZX; Sun JY; Xia XK; Wang FX
[Ad] Endereço:Department of Pharmaceutical Engineering, School of Chemistry and Chemical Engineering, Southeast University, Nanjing, P. R. China.
[Ti] Título:Three new C-27-carboxylated-lupane-triterpenoid derivatives from Potentilla discolor Bunge and their in vitro antitumor activities.
[So] Source:PLoS One;12(4):e0175502, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Three new lupane-triterpenoids (1-3) along with six known compounds (4-9) were isolated from the ethanolic extract of whole plant of Potentilla discolor Bunge. The structures of Compounds 1-3 were established by extensive 1D and 2D NMR together with other spectrum analysis, indicating that their C-27 positions were highly oxygenated, which were rarely found in nature. Their in vitro anti-proliferative activities against HepG-2, MCF-7 and T-84 cell lines were evaluated by Cell Counting Kit-8 (CCK-8) assay, and the results showed different activities for three cell lines with IC50 values ranging from 17.84 to 40.64 µM. In addition, the results from Hoechst 33258 and AO/EB staining as well as annexinV-FITC assays exhibited Compound 1 caused a markedly increased HepG-2 cellular apoptosis in a dose-dependent manner. The further mechanisms of Compound 1-induced cellular apoptosis were confirmed that 1 induced the production of ROS and the alteration of pro- and anti-apoptotic proteins, which led to the dysfunction of mitochondria and activation of caspase-9 and caspase-3 and finally caused cellular apoptosis. These results would be useful in search for new potential antitumor agents and for developing semisynthetic lupane-triterpenoid derivatives with high antitumor activity.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Ácidos Carboxílicos/química
Potentilla/química
Triterpenos/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Ensaios de Seleção de Medicamentos Antitumorais
Células Hep G2
Seres Humanos
Triterpenos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Carboxylic Acids); 0 (Triterpenes); 464-99-3 (lupane)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170907
[Lr] Data última revisão:
170907
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170408
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0175502


  4 / 164 MEDLINE  
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[PMID]:28371048
[Au] Autor:Chen PN; Yang SF; Yu CC; Lin CY; Huang SH; Chu SC; Hsieh YS
[Ad] Endereço:Institute of Biochemistry, Microbiology and Immunology, Chung Shan Medical University, Taichung, Taiwan.
[Ti] Título:Duchesnea indica extract suppresses the migration of human lung adenocarcinoma cells by inhibiting epithelial-mesenchymal transition.
[So] Source:Environ Toxicol;32(8):2053-2063, 2017 Aug.
[Is] ISSN:1522-7278
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Epithelial-mesenchymal transition (EMT) is a process through which epithelial cells are transformed into mesenchymal cells; EMT diminishes cell polarity and cell-cell adhesion in cancer cells, leading to enhanced migratory and invasive properties. In this experiment, zymography, cell invasion, and migration assays were performed. Results indicated that Duchesnea indica extracts (DIE) inhibited highly metastatic A549 and H1299 cells by reducing the secretions of matrix metalloproteinase-2 and urokinase-type plasminogen activator. Cell adhesion assay also demonstrated that DIE reduced the cell adhesion properties. Western blot analysis showed that DIE down-regulated the expression of N-cadherin, fibronectin, and vimentin, which are mesenchymal markers, and enhanced that of E-cadherin, which is an epithelial marker. In vivo study showed that tumor growth was significantly reduced in BALB/c nude mouse xenograft model administered with oral gavage of DIE. Therefore, DIE could be exhibits potential as a phytochemical-based platform for prevention and treatment of lung cancer. © 2016 Wiley Periodicals, Inc. Environ Toxicol 32: 2053-2063, 2017.
[Mh] Termos MeSH primário: Adenocarcinoma/tratamento farmacológico
Antineoplásicos Fitogênicos/farmacologia
Transição Epitelial-Mesenquimal/efeitos dos fármacos
Neoplasias Pulmonares/tratamento farmacológico
Extratos Vegetais/farmacologia
Potentilla/química
[Mh] Termos MeSH secundário: Adenocarcinoma/patologia
Animais
Antineoplásicos Fitogênicos/uso terapêutico
Caderinas/metabolismo
Adesão Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Movimento Celular/efeitos dos fármacos
Fibronectinas/metabolismo
Seres Humanos
Neoplasias Pulmonares/patologia
Metaloproteinase 2 da Matriz/metabolismo
Camundongos Endogâmicos BALB C
Camundongos Nus
Invasividade Neoplásica
Transplante de Neoplasias
Extratos Vegetais/uso terapêutico
Ativador de Plasminogênio Tipo Uroquinase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (CDH1 protein, human); 0 (Cadherins); 0 (Fibronectins); 0 (Plant Extracts); EC 3.4.21.73 (Urokinase-Type Plasminogen Activator); EC 3.4.24.24 (Matrix Metalloproteinase 2)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1002/tox.22420


  5 / 164 MEDLINE  
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[PMID]:28294523
[Au] Autor:Yuan Z; Luan G; Wang Z; Hao X; Li J; Suo Y; Li G; Wang H
[Ad] Endereço:Key Laboratory of Tibetan Medicine Research, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810008, P. R. China.
[Ti] Título:Flavonoids from Potentilla parvifolia Fisch. and Their Neuroprotective Effects in Human Neuroblastoma SH-SY5Y Cells in vitro.
[So] Source:Chem Biodivers;14(6), 2017 Jun.
[Is] ISSN:1612-1880
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Potentilla parvifolia Fisch. (Rosaceae) is a traditional medicinal plant in P. R. China. In this study, seven flavonoids, ayanin (1), tricin (2), quercetin (3), tiliroside (4), miquelianin (5), isoquercitrin (6), and astragalin (7), were separated and purified from ethyl acetate extractive fractions from ethanol extracts of P. parvifolia using a combination of sevaral chromatographic methods. The human neuroblastoma SH-SY5Y cells were differentiated with all trans-retinoic acid and treated with okadaic acid to induce tau protein phosphorylation and synaptic atrophy, which could establish an Alzheimer's disease cell model. The neuroprotective effects of these flavonoids in cellular were evaluated in vitro by this cell model. Results from the Western blot and morphology analysis suggested that compounds 3 and 4 had the better neuroprotective effects.
[Mh] Termos MeSH primário: Doença de Alzheimer/tratamento farmacológico
Flavonoides/isolamento & purificação
Neuroblastoma/tratamento farmacológico
Fármacos Neuroprotetores/isolamento & purificação
Potentilla/química
[Mh] Termos MeSH secundário: Doença de Alzheimer/induzido quimicamente
Doença de Alzheimer/patologia
Diferenciação Celular
Linhagem Celular Tumoral
Flavonoides/farmacologia
Seres Humanos
Neuroblastoma/patologia
Fármacos Neuroprotetores/farmacologia
Fosforilação
Extratos Vegetais/química
Proteínas tau/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonoids); 0 (Neuroprotective Agents); 0 (Plant Extracts); 0 (tau Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170316
[St] Status:MEDLINE
[do] DOI:10.1002/cbdv.201600487


  6 / 164 MEDLINE  
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[PMID]:28222712
[Au] Autor:Remm K; Remm L
[Ad] Endereço:Institute of Ecology and Earth Sciences, University of Tartu, Vanemuise 46, 51014, Tartu, Estonia. kalle.remm@ut.ee.
[Ti] Título:Shrubby cinquefoil (Dasiphora fruticosa (L.) Rydb.) mapping in Northwestern Estonia based upon site similarities.
[So] Source:BMC Ecol;17(1):7, 2017 Feb 21.
[Is] ISSN:1472-6785
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Different methods have been used to map species and habitat distributions. In this paper, similarity-based reasoning-a methodological approach that has received less attention-was applied to estimate the distribution and coverage of Dasiphora fruticosa for the region in the Baltic states where grows the most abundant population of this species. METHODS: Field observations, after thinning to at least 50 m interval, included 1480 coverage estimations in the species presence locations and 8317 absence locations. Species coverage for the 750 km of directly unobserved area was calculated using machine learning in the similarity-based prediction system Constud. Separate predictive sets of site features (e.g. land cover, soil type) and exemplar weights were calibrated for spatial partitions of the study area (probable presence region, unclear region, proved absence region). A modified version of the Gower's distance metric, as used in Constud, is described. RESULTS: The resulting maps depicted the predicted coverage, the certainty of decision when predicting presence or absence, and the mean similarity to the exemplar locations used while predicting. Coverage prediction errors were smaller in the unclear partition-where the species was mostly absent-than in the probable presence partition, where coverage ranged from 0 to 90%. CONCLUSIONS: We call for methodological comparisons using the same data set.
[Mh] Termos MeSH primário: Potentilla/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Ecossistema
Estônia
Solo/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Soil)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170223
[St] Status:MEDLINE
[do] DOI:10.1186/s12898-017-0117-0


  7 / 164 MEDLINE  
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[PMID]:28127806
[Au] Autor:Lee HJ; Shin JS; Lee KG; Park SC; Jang YP; Nam JH; Lee KT
[Ad] Endereço:Department of Pharmaceutical Biochemistry, College of Pharmacy, Kyung Hee University, Dongdaemun-gu, Seoul, 130-701, Korea.
[Ti] Título:Ethanol Extract of Potentilla supina Linne Suppresses LPS-induced Inflammatory Responses through NF-κB and AP-1 Inactivation in Macrophages and in Endotoxic mice.
[So] Source:Phytother Res;31(3):475-487, 2017 Mar.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In this study, we investigated the antiinflammatory effects of ethanol extracts of Potentilla. supina Linne (EPS) in lipopolysaccharide (LPS)-induced RAW 264.7 macrophages and septic mice. EPS suppressed LPS-induced nitric oxide, prostaglandin E , TNF-α, interleukin-6 and interleukin-1ß at production and mRNA levels in LPS-induced RAW 264.7 macrophages. Consistent with these observations, EPS attenuated the expressions of inducible nitric oxide synthase and cyclooxygenase-2 by downregulation of their promoter activities. Molecularly, EPS reduced the LPS-induced transcriptional activity and DNA-binding activity of nuclear factor-κB (NF-κB), and this was associated with a decrease of translocation and phosphorylation of p65 NF-κB by inhibiting the inhibitory κB-α degradation and IKK-α/ß phosphorylation. Furthermore, EPS inhibited the LPS-induced activation of activator protein-1 by reducing the expression of c-Fos and c-Jun in nuclear. EPS also suppressed the phosphorylation of mitogen-activated protein kinase, such as p38 mitogen-activated protein kinase and c-Jun N-terminal kinase. In an LPS-induced endotoxemia mouse model, pretreatment with EPS reduced the mRNA levels of inducible nitric oxide synthase, cyclooxygenase-2 and proinflammatory cytokines and increased the survival rate of mice. Collectively, these results suggest that the antiinflammatory effects of EPS were associated with the suppression of NF-κB and activator protein-1 activation and support its possible therapeutic role for the treatment of endotoxemia. Copyright © 2017 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Etanol/química
Inflamação/prevenção & controle
Lipopolissacarídeos
Macrófagos/efeitos dos fármacos
Extratos Vegetais/farmacologia
Potentilla/química
Choque Séptico/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Anti-Inflamatórios/isolamento & purificação
Anti-Inflamatórios/uso terapêutico
Linhagem Celular
Citocinas/metabolismo
Endotoxinas
Etanol/farmacologia
Inflamação/induzido quimicamente
Inflamação/metabolismo
Macrófagos/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
NF-kappa B/antagonistas & inibidores
NF-kappa B/metabolismo
Extratos Vegetais/química
Extratos Vegetais/uso terapêutico
Choque Séptico/induzido quimicamente
Choque Séptico/imunologia
Choque Séptico/metabolismo
Fator de Transcrição AP-1/antagonistas & inibidores
Fator de Transcrição AP-1/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Cytokines); 0 (Endotoxins); 0 (Lipopolysaccharides); 0 (NF-kappa B); 0 (Plant Extracts); 0 (Transcription Factor AP-1); 3K9958V90M (Ethanol)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170509
[Lr] Data última revisão:
170509
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170128
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5773


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[PMID]:27905920
[Au] Autor:Liu ZH; Wang DM; Fan SF; Li DW; Luo ZW
[Ad] Endereço:College of Forestry, Northwest A & F University, Yangling, Shaanxi, 712100, China.
[Ti] Título:Synergistic effects and related bioactive mechanism of Potentilla fruticosa L. leaves combined with Ginkgo biloba extracts studied with microbial test system (MTS).
[So] Source:BMC Complement Altern Med;16(1):495, 2016 Dec 01.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Potentilla fruticosa, also called "Jinlaomei" and "Gesanghua", is widely used as folk herbs in traditional Tibetan medicine in China to treat inflammations, wounds, certain forms of cancer, diarrhoea, diabetes and other ailments. Previous research found P. fruticosa leaf extract (C-3) combined with Ginkgo biloba extracts (EGb) showed obvious synergistic effects in a variety of oxidation systems. The aim of the present study was to further confirm the synergy of P. fruticosa combined with EGb viewed from physiological bioavailability and explore the related bioactive mechanism behind the synergism. METHODS: The microbial test system (MTS) was adopted to evaluate the related bioactive mechanism. The synergistic effects were evaluated by isobolographic analysis. The H O production rate and antioxidant enzyme (Catalase (CAT), Peroxidase (POD), Superoxide dismutase (SOD), Glutathione peroxidase (GSH-PX)) activities were determined by the colorimetric method. Enzyme gene (CAT, SOD) expression was measured by real time-PCR. RESULTS: The MTS antioxidant activity results showed the combination of C-3 + EGb exhibited synergistic effects especially at the ratio 5:1. Components of isorhamnetin and caffeic acid in C-3 and EGb displayed strong antioxidant activities on MTS and their combination also showed significant synergy in promoting H O production. The combinations of C-3 + EGb and isorhamnetin + caffeic acid promoted CAT and SOD enzyme activities and the ratio 1:1 exhibited the strongest synergy while no obvious promotion on POD and GSH-PX enzyme activities was found. Both combinations above promoted gene expression of CAT and SOD enzymes and the ratio 1:1 exhibited the strongest synergy. CONCLUSIONS: Antioxidant activity results in MTS further confirmed the significant synergy of C-3 combined with EGb and isorhamnetin combined with caffeic acid. The synergy of C-3 combined with EGb may be attributed to the combination of isorhamnetin + caffeic acid, which promoted CAT and SOD enzyme gene expression and further promoted the enzyme activities in E. coli. This study could further provide rational basis for optimizing the physiological bioavailability of P. fruticosa by using natural and safe antioxidants in low doses to produce new medicines and functional products.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Ginkgo biloba/química
Extratos Vegetais/farmacologia
Potentilla/química
[Mh] Termos MeSH secundário: Antioxidantes/uso terapêutico
Sinergismo Farmacológico
Escherichia coli/efeitos dos fármacos
Escherichia coli/enzimologia
Peróxido de Hidrogênio/metabolismo
Testes de Sensibilidade Microbiana
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161202
[St] Status:MEDLINE


  9 / 164 MEDLINE  
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[PMID]:27573158
[Au] Autor:Wan G; Tao JG; Wang GD; Liu SP; Zhao HX; Liang QD
[Ad] Endereço:Department of Orthopedics, The First Affiliated Hospital of Xinxiang Medical University, Weihui, Henan 453100, P.R. China.
[Ti] Título:In vitro antitumor activity of the ethyl acetate extract of Potentilla chinensis in osteosarcoma cancer cells.
[So] Source:Mol Med Rep;14(4):3634-40, 2016 Oct.
[Is] ISSN:1791-3004
[Cp] País de publicação:Greece
[La] Idioma:eng
[Ab] Resumo:The aim of the current study was to evaluate the anticancer effect of the ethanol extract of Potentilla chinensis, a Chinese medicinal plant. An MTT assay was used to evaluate the cell viability of MG­63 human osteosarcoma cancer cells and fR­2 cells. Furthermore, the effect of the extract on apoptosis induction, cell cycle phase distribution and inhibition of cell migration in the MG63 human osteosarcoma cancer cell line was evaluated. The effect of the extract on cell cycle phase distribution was assessed by flow cytometry using propidium iodide (PI). Phase contrast microscopy detected the morphological changes in MG63 cancer cells following extract treatment. The results of the study demonstrated that the extract was cytotoxic to MG63 cancer cells, while the normal cell line (epithelial cell line) showed lower susceptibility. Phase contrast microscopy showed distinguishing morphological features, such as cell shrinkage and blebbing induced by the extract treatment in osteosarcoma cancer cells. The average proportion of Annexin V­positive cells (total apoptotic cells) significantly increased from 5.6% in the control to 24.2, 38.8 and 55.7% in the 40, 80 and 150 µg/ml groups, respectively. The extract induced early and late apoptosis in the cancer cells. Flow cytometric analysis revealed that the extract induced G0/G1­cell cycle arrest, which also showed significant dose­dependence. The extract induced a significant and concentration­dependent reduction in cell migration. Moreover, DNA fragmentation was also examined by observation of the formation of DNA ladders. It was demonstrated that DNA fragmentation was increased with extract concentration compared with that in the control. Taken together, EEPC may serve as potential therapeutic agent against osteosarcoma, provided that the toxicity profile and in vivo investigations demonstrate that it is safe.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Apoptose/efeitos dos fármacos
Neoplasias Ósseas/tratamento farmacológico
Medicamentos de Ervas Chinesas/farmacologia
Osteossarcoma/tratamento farmacológico
Potentilla/química
[Mh] Termos MeSH secundário: Acetatos/química
Antineoplásicos Fitogênicos/química
Neoplasias Ósseas/patologia
Pontos de Checagem do Ciclo Celular/efeitos dos fármacos
Linhagem Celular Tumoral
Movimento Celular/efeitos dos fármacos
Medicamentos de Ervas Chinesas/química
Seres Humanos
Osteossarcoma/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Antineoplastic Agents, Phytogenic); 0 (Drugs, Chinese Herbal); 76845O8NMZ (ethyl acetate)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170413
[Lr] Data última revisão:
170413
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160831
[St] Status:MEDLINE
[do] DOI:10.3892/mmr.2016.5679


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[PMID]:27566693
[Au] Autor:Koski MH; Ashman TL
[Ad] Endereço:Department of Biological Sciences, University of Pittsburgh, Pittsburgh, Pennsylvania, 15260. mhk8f@virginia.edu.
[Ti] Título:Reproductive character displacement and environmental filtering shape floral variation between sympatric sister taxa.
[So] Source:Evolution;70(11):2616-2622, 2016 Nov.
[Is] ISSN:1558-5646
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Divergence in reproductive traits between closely related species that co-occur contributes to speciation by reducing interspecific gene flow. In flowering plants, greater floral divergence in sympatry than allopatry may reflect reproductive character displacement (RCD) by means of divergent pollinator-mediated selection or mating system evolution. However, environmental filtering (EF) would prevail for floral traits under stronger selection by abiotic factors than pollination, and lead to sympatric taxa being more phenotypically similar. We determine whether floral UV pigmentation and size show signatures of RCD or EF using a biogeographically informed sister taxa comparison. We determine whether 35 sister pairs in the Potentilleae tribe (Rosaceae) are allopatric or sympatric and confirm that sympatric sisters experience more similar bioclimatic conditions, an assumption of the EF hypothesis. We test whether interspecific differences are greater in allopatry or sympatry while accounting for divergence time. For UV pigmentation, sympatric sisters are more phenotypically similar than allopatric ones. For flower size, sympatric sisters show increased divergence with time since speciation but allopatric ones do not. We conclude that floral UV pigmentation shows a signature of EF, whereas flower size shows a signature of RCD. Discordant results between the traits suggest that the dominant selective agent differs between them.
[Mh] Termos MeSH primário: Flores/genética
Variação Genética
Potentilla/genética
Seleção Genética
Simpatria
[Mh] Termos MeSH secundário: Meio Ambiente
Evolução Molecular
Flores/anatomia & histologia
Especiação Genética
Pigmentação/genética
Polinização
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170630
[Lr] Data última revisão:
170630
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160828
[St] Status:MEDLINE
[do] DOI:10.1111/evo.13042



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