Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.859.937.500.625 [Categoria DeCS]
Referências encontradas : 2768 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 277 ir para página                         

  1 / 2768 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29258493
[Au] Autor:Son E; Kim SH; Yang WK; Kim DS; Cha J
[Ad] Endereço:KM Convergence Research Division, Korea Institute of Oriental Medicine, 672 Yuseong-daero, Yuseong-gu, Daejeon, 305-811, Republic of Korea.
[Ti] Título:Antiplatelet mechanism of an herbal mixture prepared from the extracts of Phyllostachys pubescens leaves and Prunus mume fruits.
[So] Source:BMC Complement Altern Med;17(1):541, 2017 Dec 19.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Bamboo (Phyllostachys pubescens) leaves and Japanese apricot (Mume fructus) fruit are traditionally recognized to be safe herbs broadly used for food and medicinal purposes in Southeast Asia. Our group previously explored their antiplatelet effects. This study was designed to confirm inhibition effects of PM21 (a 2:1 mixture of bamboo leaf extract and Japanese apricot fruit extract) on platelet aggregation and evaluate its potency to use as an herbal remedy to prevent and/or treat the diseases caused by platelet aggregation and thrombus formation. METHODS: Washed platelets were prepared and platelet aggregation was induced by adding 5 µg/mL collagen. Anti-platelet effects of PM21 (75 mg/kg, 150 mg/kg, and 300 mg/kg for ex vivo and in vivo assays, and 50, 100, 200 µg/mL for in vitro assays) were evaluated. In ex vivo assays, PM21 was orally administered to rats daily after overnight fasting for 3 days and blood was collected 1 h after the final treatment. In vivo antithrombotic effect of PM21 was observed from a carrageenan induced mouse tail thrombosis model. RESULTS: In ex vivo assay, PM21 inhibited platelet aggregation significantly. PM21 showed a strong antithrombotic effect by reducing significantly the length of mouse tail thrombus. PM21 increased intracellular cAMP level and reduced the release of ATP, TXA , and serotonin. PM21 also reduced intracellular concentration of calcium ion, fibrinogen binding to integrin α ß , and phosphorylation of ERK2, p38, PLCγ2, and PI3 K. CONCLUSIONS: PM21 showed remarkable inhibitory effects on platelet aggregation and thrombus formation. Its inhibitory function seems to influence on GPVI binding to its ligand and subsequent initiation of a signaling cascade that involves activation of effector proteins and secretion of effector molecules, such as ATP, TXA , serotonin, and Ca . PM21 also appears to exert its anti-platelet effect by deactivation of ERKs activation pathway as well as inhibition of fibrinogen binding to integrin α ß .
[Mh] Termos MeSH primário: Plaquetas/efeitos dos fármacos
Extratos Vegetais/farmacologia
Inibidores da Agregação de Plaquetas/farmacologia
Poaceae/química
Prunus/química
Trombose/metabolismo
[Mh] Termos MeSH secundário: Trifosfato de Adenosina/metabolismo
Animais
Carragenina/efeitos adversos
AMP Cíclico/metabolismo
Frutas/química
Masculino
Camundongos
Camundongos Endogâmicos ICR
Fosforilação
Folhas de Planta/química
Ratos
Ratos Sprague-Dawley
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts); 0 (Platelet Aggregation Inhibitors); 8L70Q75FXE (Adenosine Triphosphate); 9000-07-1 (Carrageenan); E0399OZS9N (Cyclic AMP)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180108
[Lr] Data última revisão:
180108
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171221
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-2032-5


  2 / 2768 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29186199
[Au] Autor:Bao W; Wuyun T; Li T; Liu H; Jiang Z; Zhu X; Du H; Bai YE
[Ad] Endereço:Non-Timber Forest Research and Development Center, Chinese Academy of Forestry, Zhengzhou, Henan, People's Republic of China.
[Ti] Título:Genetic diversity and population structure of Prunus mira (Koehne) from the Tibet plateau in China and recommended conservation strategies.
[So] Source:PLoS One;12(11):e0188685, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Prunus mira Koehne, an important economic fruit crop with high breeding and medicinal values, and an ancestral species of many cultivated peach species, has recently been declared an endangered species. However, basic information about genetic diversity, population structure, and morphological variation is still limited for this species. In this study, we sampled 420 P. mira individuals from 21 wild populations in the Tibet plateau to conduct a comprehensive analysis of genetic and morphological characteristics. The results of molecular analyses based on simple sequence repeat (SSR) markers indicated moderate genetic diversity and inbreeding (A = 3.8, Ae = 2.5, He = 0.52, Ho = 0.44, I = 0.95, FIS = 0.17) within P. mira populations. STRUCTURE, GENELAND, and phylogenetic analyses assigned the 21 populations to three genetic clusters that were moderately correlated with geographic altitudes, and this may have resulted from significantly different climatic and environmental factors at different altitudinal ranges. Significant isolation-by-distance was detected across the entire distribution of P. mira populations, but geographic altitude might have more significant effects on genetic structure than geographic distance in partial small-scale areas. Furthermore, clear genetic structure, high genetic differentiation, and restricted gene flow were detected between pairwise populations from different geographic groups, indicating that geographic barriers and genetic drift have significant effects on P. mira populations. Analyses of molecular variance based on the SSR markers indicated high variation (83.7% and 81.7%), whereas morphological analyses revealed low variation (1.30%-36.17%) within the populations. Large and heavy fruits were better adapted than light fruits and nutlets to poor climate and environmental conditions at high altitudes. Based on the results of molecular and morphological analyses, we classified the area into three conservation units and proposed several conservation strategies for wild P. mira populations in the Tibet plateau.
[Mh] Termos MeSH primário: Conservação dos Recursos Naturais
Produtos Agrícolas/genética
Variação Genética
Prunus/genética
[Mh] Termos MeSH secundário: DNA de Plantas/genética
Marcadores Genéticos
Repetições de Microssatélites
Tibet
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); 0 (Genetic Markers)
[Em] Mês de entrada:1801
[Cu] Atualização por classe:180101
[Lr] Data última revisão:
180101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171130
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0188685


  3 / 2768 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29036229
[Au] Autor:Wang J; Du J; Mu X; Wang P
[Ad] Endereço:Department of Pomology, College of Horticulture, Shanxi Agricultural University, Taigu, China.
[Ti] Título:Cloning and characterization of the Cerasus humilis sucrose phosphate synthase gene (ChSPS1).
[So] Source:PLoS One;12(10):e0186650, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Sucrose is crucial to the growth and development of plants, and sucrose phosphate synthase (SPS) plays a key role in sucrose synthesis. To understand the genetic and molecular mechanisms of sucrose synthesis in Cerasus humilis, ChSPS1, a homologue of SPS, was cloned using RT-PCR. Sequence analysis showed that the open reading frame (ORF) sequence of ChSPS1 is 3174 bp in length, encoding a predicted protein of 1057 amino acids. The predicted protein showed a high degree of sequence identity with SPS homologues from other species. Real-time RT-PCR analysis showed that ChSPS1 mRNA was detected in all tissues and the transcription level was the highest in mature fruit. There is a significant positive correlation between expression of ChSPS1 and sucrose content. Prokaryotic expression of ChSPS1 indicated that ChSPS1 protein was expressed in E. coli and it had the SPS activity. Overexpression of ChSPS1 in tobacco led to upregulation of enzyme activity and increased sucrose contents in transgenic plants. Real-time RT-PCR analysis showed that the expression of ChSPS1 in transgenic tobacco was significantly higher than in wild type plants. These results suggested that ChSPS1 plays an important role in sucrose synthesis in Cerasus humilis.
[Mh] Termos MeSH primário: Glucosiltransferases/genética
Prunus/enzimologia
Prunus/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Clonagem Molecular
Regulação da Expressão Gênica de Plantas
Glucosiltransferases/química
Glucosiltransferases/metabolismo
Análise de Sequência
Sacarose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
57-50-1 (Sucrose); EC 2.4.1.- (Glucosyltransferases); EC 2.4.1.14 (sucrose-phosphate synthase)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171017
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186650


  4 / 2768 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29020049
[Au] Autor:Gao R; Xu Y; Candresse T; He Z; Li S; Ma Y; Lu M
[Ad] Endereço:State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
[Ti] Título:Further insight into genetic variation and haplotype diversity of Cherry virus A from China.
[So] Source:PLoS One;12(10):e0186273, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cherry virus A (CVA) infection appears to be prevalent in cherry plantations worldwide. In this study, the diversity of CVA isolates from 31 cherry samples collected from different orchards around Bohai Bay in northeastern China was analyzed. The complete genome of one of these isolates, ChYT52, was found to be 7,434 nt in length excluding the poly (A) tail. It shares between 79.9-98.7% identity with CVA genome sequences in GenBank, while its RdRp core is more divergent (79.1-90.7% nt identity), likely as a consequence of a recombination event. Phylogenetic analysis of ChYT52 genome with CVA genomes in Genbank resulted in at least 7 major clusters plus additional 5 isolates alone at the end of long branches suggesting the existence of further phylogroups diversity in CVA. The genetic diversity of Chinese CVA isolates from 31 samples and GenBank sequences were analyzed in three genomic regions that correspond to the coat protein, the RNA-dependent RNA polymerase core region, and the movement protein genes. With few exceptions likely representing further recombination impact, the trees various trees are largely congruent, indicating that each region provides valuable phylogenetic information. In all cases, the majority of the Chinese CVA isolates clustering in phylogroup I, together with the X82547 reference sequence from Germany. Statistically significant negative values were obtained for Tajima's D in the three genes for phylogroup I, suggesting that it may be undergoing a period of expansion. There was considerable haplotype diversity in the individual samples and more than half samples contained genetically diverse haplotypes belonging to different phylogroups. In addition, a number of statistically significant recombination events were detected in CVA genomes or in the partial genomic sequences indicating an important contribution of recombination to CVA evolution. This work provides a foundation for elucidation of the epidemiological characteristics and evolutionary history of CVA populations.
[Mh] Termos MeSH primário: Variação Genética
Haplótipos/genética
Vírus de Plantas/genética
[Mh] Termos MeSH secundário: Sequência de Bases
China
Genoma Viral
Nucleotídeos/genética
Filogenia
Doenças das Plantas/virologia
Vírus de Plantas/isolamento & purificação
Prunus/virologia
Reação em Cadeia da Polimerase em Tempo Real
Recombinação Genética/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Nucleotides)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171012
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0186273


  5 / 2768 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28881950
[Au] Autor:Bernardi D; Lazzari JC; Andreazza F; Mayer NA; Botton M; Nava DE
[Ad] Endereço:Laboratory of Entomology, Embrapa Clima Temperado, BR 392?Km 78, Caixa Postal 403, Pelotas, RS 96010-971, Brazil.
[Ti] Título:Susceptibility, Oviposition Preference, and Biology of Grapholita molesta (Lepidoptera: Tortricidae) in Prunus Spp. Rootstock Genotypes.
[So] Source:Environ Entomol;46(4):871-877, 2017 08 01.
[Is] ISSN:1938-2936
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Studying the susceptibility of peach trees to Grapholita molesta (Busck) is one of the major steps in the development of pest-resistant peach varieties. This work evaluated the susceptibility of 55 genotypes of the "Prunus Rootstock Collection" ("Coleção Porta-enxerto de Prunus") of Embrapa Temperate Climate (Pelotas, Rio Grande do Sul, Brazil) to the natural infestation of G. molesta, assessed the oviposition preference of G. molesta in choice and no-choice bioassays, and estimated the biological parameters and the fertility life table on different Prunus spp. genotypes in the laboratory. Genotypes Prunus kansuensis (Rehder), I-67-52-9, and I-67-52-4 were the most susceptible to G. molesta infestation in the field (>60% of branches infested), while 'Sharpe' (Prunus angustifolia x Prunus spp.) and Prunus sellowii (Koehne) were the least infested (0% of branches infested). In choice and no-choice bioassays, G. molesta preferred to oviposit on P. kansuensis when compared with Sharpe. The Sharpe genotype also showed an antibiosis effect, resulting in negative effects on the fertility life table parameters when compared with the genotypes P. kansuensis and 'Capdeboscq.' The results found in the present study can provide information to initiate a long-term breeding program moving desired G. molesta resistance traits from the rootstock into the Prunus spp. cultivars.
[Mh] Termos MeSH primário: Herbivoria
Mariposas/fisiologia
Oviposição
Prunus/genética
[Mh] Termos MeSH secundário: Animais
Brasil
Genótipo
Interações Hospedeiro-Patógeno
Larva/crescimento & desenvolvimento
Larva/fisiologia
Traços de História de Vida
Mariposas/crescimento & desenvolvimento
Raízes de Plantas/fisiologia
Prunus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171126
[Lr] Data última revisão:
171126
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170909
[St] Status:MEDLINE
[do] DOI:10.1093/ee/nvx114


  6 / 2768 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28831862
[Au] Autor:Pi K; Lee K
[Ad] Endereço:a Bio Center , Incheon Business Information Technopark , Incheon , Republic of Korea.
[Ti] Título:Prunus mume extract exerts antioxidant activities and suppressive effect of melanogenesis under the stimulation by alpha-melanocyte stimulating hormone in B16-F10 melanoma cells.
[So] Source:Biosci Biotechnol Biochem;81(10):1883-1890, 2017 Oct.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In the current study, we examined the antioxidant and skin-whitening properties of Prunus mume extract (PME). The ability of PME to scavenge 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals was investigated in vitro. At a concentration of 1000 µg/mL, PME neutralized >45% free radical activity. Cell viability assessment with the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay revealed that at concentrations <1500 µg/mL, PME does not exert cytotoxic effects on murine B16 melanoma (B16) cells. Morphological analysis disclosed that melanin production is inhibited in B16 cells treated with 250 nM α-melanocyte-stimulating hormone (α-MSH) and PME. We conclude that fruit extracts of P. mume exert a skin-whitening effect by inhibiting melanin production via regulation of melanogenesis-associated protein expression in melanocytes.
[Mh] Termos MeSH primário: Depuradores de Radicais Livres/farmacologia
Melaninas/biossíntese
Melanoma Experimental/patologia
Extratos Vegetais/farmacologia
Prunus/química
alfa-MSH/farmacologia
[Mh] Termos MeSH secundário: Animais
Compostos de Bifenilo/química
Linhagem Celular Tumoral
Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Depuradores de Radicais Livres/química
Depuradores de Radicais Livres/isolamento & purificação
Melanossomas/efeitos dos fármacos
Melanossomas/metabolismo
Camundongos
Estresse Oxidativo/efeitos dos fármacos
Picratos/química
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biphenyl Compounds); 0 (Free Radical Scavengers); 0 (Melanins); 0 (Picrates); 0 (Plant Extracts); 581-05-5 (alpha-MSH); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170824
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1365591


  7 / 2768 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28632759
[Au] Autor:Kinoti WM; Constable FE; Nancarrow N; Plummer KM; Rodoni B
[Ad] Endereço:Agriculture Victoria, AgriBio, La Trobe University, Melbourne, VIC, Australia.
[Ti] Título:Analysis of intra-host genetic diversity of Prunus necrotic ringspot virus (PNRSV) using amplicon next generation sequencing.
[So] Source:PLoS One;12(6):e0179284, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:PCR amplicon next generation sequencing (NGS) analysis offers a broadly applicable and targeted approach to detect populations of both high- or low-frequency virus variants in one or more plant samples. In this study, amplicon NGS was used to explore the diversity of the tripartite genome virus, Prunus necrotic ringspot virus (PNRSV) from 53 PNRSV-infected trees using amplicons from conserved gene regions of each of PNRSV RNA1, RNA2 and RNA3. Sequencing of the amplicons from 53 PNRSV-infected trees revealed differing levels of polymorphism across the three different components of the PNRSV genome with a total number of 5040, 2083 and 5486 sequence variants observed for RNA1, RNA2 and RNA3 respectively. The RNA2 had the lowest diversity of sequences compared to RNA1 and RNA3, reflecting the lack of flexibility tolerated by the replicase gene that is encoded by this RNA component. Distinct PNRSV phylo-groups, consisting of closely related clusters of sequence variants, were observed in each of PNRSV RNA1, RNA2 and RNA3. Most plant samples had a single phylo-group for each RNA component. Haplotype network analysis showed that smaller clusters of PNRSV sequence variants were genetically connected to the largest sequence variant cluster within a phylo-group of each RNA component. Some plant samples had sequence variants occurring in multiple PNRSV phylo-groups in at least one of each RNA and these phylo-groups formed distinct clades that represent PNRSV genetic strains. Variants within the same phylo-group of each Prunus plant sample had ≥97% similarity and phylo-groups within a Prunus plant sample and between samples had less ≤97% similarity. Based on the analysis of diversity, a definition of a PNRSV genetic strain was proposed. The proposed definition was applied to determine the number of PNRSV genetic strains in each of the plant samples and the complexity in defining genetic strains in multipartite genome viruses was explored.
[Mh] Termos MeSH primário: Biomarcadores/metabolismo
Variação Genética/genética
Sequenciamento de Nucleotídeos em Larga Escala/métodos
Ilarvirus/patogenicidade
Doenças das Plantas/virologia
Prunus/virologia
[Mh] Termos MeSH secundário: Ilarvirus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biomarkers)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170928
[Lr] Data última revisão:
170928
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170621
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0179284


  8 / 2768 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
[PMID]:28574393
[Au] Autor:Chichoyan N
[Ad] Endereço:Yerevan state medical university after M. Heratsi, Department of Pharmacognosy, Armenia.
[Ti] Título:[RATIONAL USE APRICOT GUM (GUMMI ARMENIACAE) IN REPUBLIC OF ARMENIA].
[So] Source:Georgian Med News;(265):109-114, 2017 Apr.
[Is] ISSN:1512-0112
[Cp] País de publicação:Georgia (Republic)
[La] Idioma:rus
[Ab] Resumo:Till nowadays the sources of gums of Armenian flora are remaining unutilized because of absence of resoursological and phytochemical research as well as standards corresponding. Amongst the camed-bearing trees of the flora of Armenia (apricot, peach, plum, almond) and the other families representatives (eleagnus, tragakant) the apricot trees (Armeniaca vulgaris Lam.) became an object of our great interests, considering its being as the most important cultivating fruit-bearing trees in Armenia and the fact, that they cover significant areas in the Ararat valley, Kotayk, Aragatsotn and the footings of the Vayk region. The goal of our investigation was the pharmacognostic (resoursological, commodity and analytical, phythochemical) complex evaluation encountering and the apricot tree camed's (gummi armeniacae) biological activity evaluation, as well as the natural resourses rational concepts elaboration. On the base of the investigation results got a concept of rational use of the apricot gums was developed, which assumes the local sources enlargement, as well as its wide application in medicine and in different aspects of industry.
[Mh] Termos MeSH primário: Gomas Vegetais
Prunus
[Mh] Termos MeSH secundário: Anti-Infecciosos/química
Anti-Infecciosos/isolamento & purificação
Antioxidantes/química
Antioxidantes/isolamento & purificação
Armênia
Compostos Fitoquímicos/química
Compostos Fitoquímicos/isolamento & purificação
Gomas Vegetais/química
Gomas Vegetais/normas
Polissacarídeos/química
Polissacarídeos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antioxidants); 0 (Phytochemicals); 0 (Plant Gums); 0 (Polysaccharides)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171110
[Lr] Data última revisão:
171110
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170603
[St] Status:MEDLINE


  9 / 2768 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28547891
[Au] Autor:Cui N; Yang PC; Guo K; Kang L; Cui F
[Ad] Endereço:State Key Laboratory of Integrated Management of Pest Insects & Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China.
[Ti] Título:Large-scale gene expression reveals different adaptations of Hyalopterus persikonus to winter and summer host plants.
[So] Source:Insect Sci;24(3):431-442, 2017 Jun.
[Is] ISSN:1744-7917
[Cp] País de publicação:Australia
[La] Idioma:eng
[Ab] Resumo:Host alternation, an obligatory seasonal shifting between host plants of distant genetic relationship, has had significant consequences for the diversification and success of the superfamily of aphids. However, the underlying molecular mechanism remains unclear. In this study, the molecular mechanism of host alternation was explored through a large-scale gene expression analysis of the mealy aphid Hyalopterus persikonus on winter and summer host plants. More than four times as many unigenes of the mealy aphid were significantly upregulated on summer host Phragmites australis than on winter host Rosaceae plants. In order to identify gene candidates related to host alternation, the differentially expressed unigenes of H. persikonus were compared to salivary gland expressed genes and secretome of Acyrthosiphon pisum. Genes involved in ribosome and oxidative phosphorylation and with molecular functions of heme-copper terminal oxidase activity, hydrolase activity and ribosome binding were potentially upregulated in salivary glands of H. persikonus on the summer host. Putative secretory proteins, such as detoxification enzymes (carboxylesterases and cytochrome P450s), antioxidant enzymes (peroxidase and superoxide dismutase), glutathione peroxidase, glucose dehydrogenase, angiotensin-converting enzyme, cadherin, and calreticulin, were highly expressed in H. persikonus on the summer host, while a SCP GAPR-1-like family protein and a salivary sheath protein were highly expressed in the aphids on winter hosts. These results shed light on phenotypic plasticity in host utilization and seasonal adaptation of aphids.
[Mh] Termos MeSH primário: Adaptação Biológica
Afídeos/metabolismo
Herbivoria
Proteínas de Insetos/metabolismo
[Mh] Termos MeSH secundário: Animais
Afídeos/genética
Perfilação da Expressão Gênica
Proteínas de Insetos/genética
Prunus
Glândulas Salivares/metabolismo
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170828
[Lr] Data última revisão:
170828
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE
[do] DOI:10.1111/1744-7917.12336


  10 / 2768 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28484361
[Au] Autor:Liu J; Wang Y; Li Q
[Ad] Endereço:College of Horticulture, Sichuan Agricultural University, Chengdu, Sichuan 611130 People's Republic of China.
[Ti] Título:Analysis of differentially expressed genes and adaptive mechanisms of Lindl. under alkaline stress.
[So] Source:Hereditas;154:10, 2017.
[Is] ISSN:1601-5223
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Lindl. is a naturally salt-alkaline-tolerant plant with several unique characteristics, and it can be used as the rootstock of Chinese plum ( Lindl.) in saline-alkaline soils. To comprehensively investigate the alkaline acclimation mechanisms in , a series of analyses were conducted under alkaline stress, including analyses of the kinetics of molecular and physiological changes, and leaf microstructure. RESULTS: To understand the kinetics of molecular changes under short-term alkaline stress, we used Illumina HiSeq 2500 platform to identify alkaline stress-related differentially expressed genes (DEGs) in . Approximately 53.0 million high-quality clean reads were generated from 59.6 million raw reads, and a total of 124,786 unigenes were obtained after assembly of transcriptome data. After alkaline stress treatment, a total of 8948 unigenes were identified as DEGs. Based on these DEGs, a Gene Ontology (GO) enrichment analysis was conducted, suggesting that 28 genes may play an important role in the early alkaline stress response. In addition, analysis of DEGs with the Kyoto Encyclopedia of Genes and Genomes (KEGG) revealed that pathways were significant at different treatment time points. A significant positive correlation was found between the quantitative real-time PCR (qRT-PCR) results and the RNA-Seq data for seven alkaline-related genes, confirming the reliability of the RNA-Seq results. Based on physiological analysis of in response to long-term alkaline stress, we found that the internal microstructures of the leaves of changed to adapt to long-term alkaline stress. Various physiological indexes indicated that the degree of membrane injury increased with increasing duration of alkaline stress, affecting photosynthesis in seedlings. CONCLUSIONS: This represents the first investigation into the physiology and transcriptome of in response to alkaline stress. The results of this study can enrich the genomic resources available for , as well as deepening our understanding of molecular and physiological alkaline tolerance mechanisms in . This will also provide new insights into our understanding of alkaline acclimation mechanisms in Chinese plum ( ) trees.
[Mh] Termos MeSH primário: Aclimatação/genética
Prunus/genética
Estresse Fisiológico/genética
[Mh] Termos MeSH secundário: Álcalis
Regulação da Expressão Gênica de Plantas
Concentração de Íons de Hidrogênio
Folhas de Planta/fisiologia
Prunus/fisiologia
Reação em Cadeia da Polimerase em Tempo Real
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkalies)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170601
[Lr] Data última revisão:
170601
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170510
[St] Status:MEDLINE
[do] DOI:10.1186/s41065-017-0031-7



página 1 de 277 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde