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[PMID]: | 28535789 |
[Au] Autor: | Islam NU; Amin R; Shahid M; Amin M; Zaib S; Iqbal J |
[Ad] Endereço: | Department of Pharmacy, Sarhad University of Science and Information Technology, Peshawar, 25000, Pakistan. islanaz@yahoo.com. |
[Ti] Título: | A multi-target therapeutic potential of Prunus domestica gum stabilized nanoparticles exhibited prospective anticancer, antibacterial, urease-inhibition, anti-inflammatory and analgesic properties. |
[So] Source: | BMC Complement Altern Med;17(1):276, 2017 May 23. | [Is] ISSN: | 1472-6882 |
[Cp] País de publicação: | England |
[La] Idioma: | eng |
[Ab] Resumo: | BACKGROUND: Phytotherapeutics exhibit diverse pharmacological effects that are based on the combined action of a mixture of phytoconstituents. In this study, Prunus domestica gum-loaded, stabilized gold and silver nanoparticles (Au/Ag-NPs) were evaluated for their prospective anticancer, antibacterial, urease-inhibition, anti-inflammatory, and analgesic properties. METHODS: Au/Ag-NPs were biosynthesized and characterized with UV-Vis, FTIR, SEM, EDX, and XRD techniques. The effect of gum and metal ion concentration, reaction temperature, and time on the synthetic stability of nanoparticles was studied along with their post-synthetic stability against varying pH and salt concentrations, long-term storage and extremes of temperature. Nanoparticles were tested for anticancer (HeLa cervical cancer cells), antibacterial (Staphylococcus aureus, Escherichia coli, and Pseudomonas aeruginosa), urease inhibition (jack-bean urease), anti-inflammatory (carrageenan-induced paw edema), and antinociceptive (abdominal constriction response) activities. RESULTS: The nanoparticles were mostly spherical with an average particle size between 7 and 30 nm (Au-NPs) and 5-30 nm (Ag-NPs). Au/Ag-NPs maintained their colloidal stability and nanoscale characteristics against variations in physicochemical factors. Au/Ag-NPs have potent anticancer potential (IC = 2.14 ± 0.15 µg/mL and 3.45 ± 0.23 µg/mL). Au/Ag-NPs selectively suppressed the growth of S. aureus (10.5 ± 0.6 mm, 19.7 ± 0.4 mm), E. coli (10 ± 0.4 mm, 14.4 ± 0.7 mm), and P. aeruginosa (8.2 ± 0.3 mm, 13.1 ± 0.2 mm), as well as showed preferential inhibition against jack-bean urease (19.2 ± 0.86%, 21.5 ± 1.17%). At doses of 40 and 80 mg/kg, Au-NPs significantly ameliorated the increase in paw edema during the 1st h (P < 0.05, P < 0.01) and 2-5 h (P < 0.001) of carrageenan-induced inflammation compared to the 200 and 400 mg/kg doses of P. domestica gum (P < 0.05, P < 0.001). At similar doses, Au-NPs also significantly abolished (P < 0.01) the tonic visceral, chemically-induced nociception, which was comparable to that of P. domestica gum (200 mg/kg; P < 0.05, 400 mg/kg; P < 0.01). |
[Mh] Termos MeSH primário: |
Analgésicos/química Antibacterianos/química Anti-Inflamatórios/química Inibidores Enzimáticos/química Extratos Vegetais/administração & dosagem Gomas Vegetais/administração & dosagem Prunus domestica/química
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[Mh] Termos MeSH secundário: |
Analgésicos/administração & dosagem Analgésicos/síntese química Animais Antibacterianos/administração & dosagem Antibacterianos/síntese química Anti-Inflamatórios/administração & dosagem Anti-Inflamatórios/síntese química Bactérias/efeitos dos fármacos Linhagem Celular Tumoral Edema/tratamento farmacológico Inibidores Enzimáticos/administração & dosagem Inibidores Enzimáticos/síntese química Feminino Ouro/administração & dosagem Ouro/química Seres Humanos Masculino Camundongos Camundongos Endogâmicos BALB C Nanopartículas/administração & dosagem Nanopartículas/química Extratos Vegetais/química Gomas Vegetais/química Prata/administração & dosagem Prata/química Urease/antagonistas & inibidores Urease/metabolismo
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[Pt] Tipo de publicação: | JOURNAL ARTICLE |
[Nm] Nome de substância:
| 0 (Analgesics); 0 (Anti-Bacterial Agents); 0 (Anti-Inflammatory Agents); 0 (Enzyme Inhibitors); 0 (Plant Extracts); 0 (Plant Gums); 3M4G523W1G (Silver); 7440-57-5 (Gold); EC 3.5.1.5 (Urease) |
[Em] Mês de entrada: | 1708 |
[Cu] Atualização por classe: | 170822 |
[Lr] Data última revisão:
| 170822 |
[Sb] Subgrupo de revista: | IM |
[Da] Data de entrada para processamento: | 170525 |
[St] Status: | MEDLINE |
[do] DOI: | 10.1186/s12906-017-1791-3 |
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