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[PMID]:29372790
[Au] Autor:Suprun II; Tokmakov SV; Bandurko IA; Ilnitskaya ET
[Ti] Título:[SSR polymorphism of modern cultivars and autochthonous forms of the pear tree from North Caucasus].
[So] Source:Genetika;52(11):1270-8, 2016 Nov.
[Is] ISSN:0016-6758
[Cp] País de publicação:Russia (Federation)
[La] Idioma:rus
[Ab] Resumo:Genetic similarity and relatedness within the set of pear genotypes including autochthonous Circassian cultivars from North Caucasus, European cultivars, accessions of Pyrus caucasica Fed., and modern Russian cultivars were estimated on the basis of analysis of SSR loci. The level of polymorphism for the studied loci varied from 11 to 15 alleles per locus in the set of 29 samples of pears. A higher level of allelic polymorphism of SSR loci was revealed for a set of P. caucasica samples in comparison with modern cultivated cultivars: from 9 to 12 alleles for P. caucasica and from 6 to 8 alleles for modern cultivars. Specific alleles for the mentioned groups of pears were identified. UPGMA clustering revealed two distinct groups: one includes P. caucasica accessions and autochthonous Caucasian cultivars and the other group includes all cultivated European and Russian pear cultivar. The results support the hypothesis of an isolated gene pool formation of autochthonous pear cultivars of the North Caucasus and their probable origin from the wild P. caucasica.
[Mh] Termos MeSH primário: Alelos
Loci Gênicos
Repetições de Microssatélites
Polimorfismo Genético
Pyrus/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180205
[Lr] Data última revisão:
180205
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180127
[St] Status:MEDLINE


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[PMID]:29088238
[Au] Autor:Zhang J; Cheng X; Jin Q; Su X; Li M; Yan C; Jiao X; Li D; Lin Y; Cai Y
[Ad] Endereço:School of Life Science, Anhui Agricultural University, Hefei, China.
[Ti] Título:Comparison of the transcriptomic analysis between two Chinese white pear (Pyrus bretschneideri Rehd.) genotypes of different stone cells contents.
[So] Source:PLoS One;12(10):e0187114, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Stone cell content is thought to be one of the key determinants for fruit quality in pears. However, the molecular mechanism of stone cell development remains poorly understood. In this study, we found that the stone cell clusters (SCCs) distribution and area in 'Dangshan Su' (with abundant stone cells) were higher as compared to 'Lianglizaosu' (low stone cell content bud sport of 'Dangshan Su') based on the histochemical staining, and the correlations of lignin content with stone cell content and SCC area was significant. The fruits of 'Dangshan Su' and 'Lianglizaosu' at three different developmental stages (23 and 55 days after flowering and mature) were sampled for comparative transcriptome analysis to explore the metabolic pathways associated with stone cell development. A total of 42444 unigenes were obtained from two varieties, among which 7203 differentially expressed genes (DEGs) were identified by comparison of the six transcriptomes. Specifically, many DEGs associated with lignin biosynthesis were identified, including coumaroylquinate 3-monooxygenase (C3H), shikimate O-hydroxycinnamoyltransferase (HCT), ferulate 5-hydroxylase (F5H), cinnamyl alcohol dehydrogenase (CAD) and peroxidase (POD), as well as genes related to carbon metabolism, such as sorbitol dehydrogenase-like (SDH-like) and ATP-dependent 6-phosphofructokinase (ATP-PFK). At the peak of the stone cell content (55 days after flowering), the expression level of these genes in 'Dangshan Su' was significantly increased compared with 'Lianglizaosu', indicating that these genes were closely related to stone cell development. We validated the transcriptional levels of 33 DEGs using quantitative real-time polymerase chain reaction (qRT-PCR) analysis. The results were consistent with the transcriptome analysis, indicating the reliability of transcriptome data. In addition, subcellular localization analysis of three DEGs in lignin synthesis (PbC3H, PbF5H and PbPOD) revealed that these proteins are mainly distributed in the cell membrane and cytoplasm. These results provide new insights into the molecular mechanism of stone cell formation.
[Mh] Termos MeSH primário: Frutas/citologia
Pyrus/genética
[Mh] Termos MeSH secundário: Frutas/crescimento & desenvolvimento
Frutas/metabolismo
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Estudos de Associação Genética
Lignina/metabolismo
Redes e Vias Metabólicas/genética
Pyrus/metabolismo
Reação em Cadeia da Polimerase em Tempo Real
Análise de Sequência de DNA
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
9005-53-2 (Lignin)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171101
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0187114


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[PMID]:28934298
[Au] Autor:Kan J; Liu T; Ma N; Li H; Li X; Wang J; Zhang B; Chang Y; Lin J
[Ad] Endereço:Institute of Horticulture, Jiangsu Academy of Agricultural Sciences/ Jiangsu Key Laboratory for Horticultural Crop Genetic improvement, Nanjing, China.
[Ti] Título:Transcriptome analysis of Callery pear (Pyrus calleryana) reveals a comprehensive signalling network in response to Alternaria alternata.
[So] Source:PLoS One;12(9):e0184988, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The pear is an important temperate fruit worldwide that is produced by a group of species in the genus Pyrus. Callery pear (Pyrus calleryana Decne) is characterized by high resistance to multiple diseases, good adaptability, and high ornamental value, and is therefore widely planted in pear orchards for edible fruit production or as stock. Plant pathogens are a major threat to pear yield. Black spot disease, caused by the filamentous fungus Alternaria alternata, is one of the most serious diseases in pear. Elucidation of resistant genes to black spot disease is extremely important for understanding the underlying mechanisms as well as for the development of resistant cultivars. In this study, high-throughput single-strand RNA-sequencing was used to compare the transcriptome profiles of Callery pear leaves before and after A. alternata incubation for 7 days. The analysis yielded 73.3 Gb of clean data that were mapped onto the reference genome of the Chinese pear, and differentially expressed gene(DEG)s were identified with |log2FC| ≥ 1. Functional annotation demonstrated that black spot disease promoted great changes in the overall metabolism, and enrichment analysis of gene ontology terms showed that most of them are closely linked to signalling network and photosynthesis. Specifically, the genes included mainly transcription factors and genes involved in calcium signalling and ethylene and jasmonate pathways. Eight members of the ethylene response factor transcription factor gene family Group IX, including ERF1, ERF7, and ERF105, were up-regulated to 2.03-3.37-fold compared with CK, suggesting their role in the defence response to pathogen infection. Additionally, multiple transcription factors involved in biotic stresses, such as NAC78, NAC2, MYB44, and bHLH28, were up-regulated. Furthermore, we identified 144 long non-coding (lnc)RNAs, providing new insight into the involvement of lncRNAs in the response to black spot disease. Our study provides valuable data on the molecular genetics and functional genomic mechanisms of resistance to black spot disease in Callery pear. A good understanding of the molecular response to this disease will allow the development of durable and environmentally friendly control strategies.
[Mh] Termos MeSH primário: Alternaria/patogenicidade
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Redes Reguladoras de Genes
Doenças das Plantas/genética
Proteínas de Plantas/genética
Pyrus/genética
[Mh] Termos MeSH secundário: Sequenciamento de Nucleotídeos em Larga Escala
Doenças das Plantas/microbiologia
Pyrus/microbiologia
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170922
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0184988


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[PMID]:28891001
[Au] Autor:Xiang J; Fu M; Hong N; Zhai L; Xiao F; Wang G
[Ad] Endereço:State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, 430070, Hubei, China.
[Ti] Título:Characterization of a novel botybirnavirus isolated from a phytopathogenic Alternaria fungus.
[So] Source:Arch Virol;162(12):3907-3911, 2017 Dec.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Alternaria fungi are important pathogens infecting a wide variety of organisms. Here, we report a novel double-stranded RNA (dsRNA) mycovirus named Alternaria botybirnavirus 1 (ABRV1) isolated from a phytopathogenic Alternaria sp. strain (SCFS-3) infecting a pear tree in China. ABRV1 has two dsRNA components (dsRNAs 1 and 2) with the sizes of 6,188 and 5,903 bp, containing two putative open reading frames encoding two polyproteins (202 and 192 kDa, respectively). The polyprotein encoded by ABRV1 dsRNA1 shares 41% amino acid (aa) sequence identity with the one encoded by dsRNA2 (instead of dsRNA1) of Sclerotinia sclerotiorum botybirnavirus 1 (SsBRV1). Conversely, the polyprotein encoded by ABRV1 dsRNA2 shares 46% aa sequence identity with the one (i.e., cap-pol fusion protein) encoded by SsBRV1 dsRNA1. ABRV1 has isometric spherical virus particles (~40 nm in diameter), putatively composed of the 60-, 70- and 80-kDa structural proteins. The genomic organization and phylogenetic analyses revealed that ABRV1 belongs to a newly proposed family "Botybirnaviridae", and to our knowledge, this is the first report of a botybirnavirus infecting an Alternaria sp. strain.
[Mh] Termos MeSH primário: Alternaria/virologia
Micovírus/classificação
Micovírus/isolamento & purificação
Vírus de RNA/classificação
Vírus de RNA/isolamento & purificação
[Mh] Termos MeSH secundário: China
Micovírus/genética
Ordem dos Genes
Genoma Viral
Peso Molecular
Fases de Leitura Aberta
Filogenia
Pyrus/microbiologia
Vírus de RNA/genética
RNA de Cadeia Dupla/genética
RNA Viral/genética
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Proteínas Virais/química
Proteínas Virais/genética
Vírion/química
Vírion/ultraestrutura
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Double-Stranded); 0 (RNA, Viral); 0 (Viral Proteins)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170912
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-017-3543-6


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[PMID]:28854662
[Au] Autor:Atlihan R; Kasap I; Özgökçe MS; Polat-Akköprü E; Chi H
[Ad] Endereço:Department of Plant Protection, Faculty of Agriculture, University of Yuzuncu Yil, 65080 Van, Turkey.
[Ti] Título:Population Growth of Dysaphis pyri (Hemiptera: Aphididae) on Different Pear Cultivars With Discussion on Curve Fitting in Life Table Studies.
[So] Source:J Econ Entomol;110(4):1890-1898, 2017 Aug 01.
[Is] ISSN:1938-291X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Population growth parameters of the Dysaphis pyri (Boyer de Fonscolombe) (Hemiptera: Aphididae) were evaluated on four different cultivars (Coscia, Ankara, Williams, and Santa-Maria) of pear (Pyrus communis L.) under field conditions in the Van region of Turkey. Aphids were kept on leaves of 10-yr-old pear trees in Plexiglas clip-cells (20 mm in diameter and 10 mm in height, with the upper side covered with muslin). For the description of the stage differentiation during population growth, we analyzed raw data of developmental time, survival, and fecundity using the age-stage, two-sex life table to take the variable developmental rate among individuals into account. Results indicated that the Coscia and Ankara cultivars are less favorable hosts for D. pyri because of the longer preadult developmental time, higher preadult mortality rate, and lower total fecundity on these cultivars. The intrinsic rate of increase (r), the net reproduction rate (R0), and the finite rate of increase (λ) values were lower on the Coscia and Ankara cultivars. We discussed the application of the Weibull function, polynomial model, and Enkegaard model in life table studies. Because these models are often inaccurate in describing survival and reproduction parameters, we suggest that their application in life table research should be reevaluated.
[Mh] Termos MeSH primário: Afídeos/fisiologia
Cadeia Alimentar
Pyrus
[Mh] Termos MeSH secundário: Animais
Afídeos/crescimento & desenvolvimento
Feminino
Fertilidade
Tábuas de Vida
Longevidade
Masculino
Ninfa/crescimento & desenvolvimento
Ninfa/fisiologia
Crescimento Demográfico
Pyrus/genética
Pyrus/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170901
[St] Status:MEDLINE
[do] DOI:10.1093/jee/tox174


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[PMID]:28844143
[Au] Autor:Zhang Z; Zhang Q; Gao B; Gou G; Li L; Shi H; Wang M
[Ad] Endereço:Department of Pesticide Science, College of Plant Protection, Nanjing Agricultural University, State & Local Joint Engineering Research Center of Green Pesticide Invention and Application , Nanjing 210095, China.
[Ti] Título:Simultaneous Enantioselective Determination of the Chiral Fungicide Prothioconazole and Its Major Chiral Metabolite Prothioconazole-Desthio in Food and Environmental Samples by Ultraperformance Liquid Chromatography-Tandem Mass Spectrometry.
[So] Source:J Agric Food Chem;65(37):8241-8247, 2017 Sep 20.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An efficient and sensitive chiral analytical method was established for the determination of the chiral fungicide prothioconazole and its major chiral metabolite prothioconazole-desthio in agricultural and environmental samples using ultraperformance liquid chromatography-tandem mass spectrometry. The optical rotation and absolute configuration of enantiomers were identified by optical rotation detector and electronic circular dichroism spectra. The elution order of prothioconazole and its chiral metabolite enantiomers was R-(+)-prothioconazole-desthio, S-(-)-prothioconazole-desthio, R-(-)-prothioconazole, and S-(+)-prothioconazole. The mean recoveries from the samples was 71.8-102.0% with intraday relative standard deviations (RSDs) of 0.3-11.9% and interday RSDs of 0.9-10.6%. The formation of prothioconazole-desthio was studied in soil under field conditions and enantioselective degradation was observed for chiral prothioconazole. Remarkable enantioselective degradation was observed: R-prothioconazole degraded preferentially with EF values from 0.48 to 0.37. Although prothioconazole-desthio is the most remarkably bioactive metabolite, no obvious enantioselective behavior was observed in soil. These results may help to systematically evaluate prothioconazole and its metabolites in food and environmental safety.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Contaminação de Alimentos/análise
Fungicidas Industriais/química
Poluentes do Solo/química
Espectrometria de Massas em Tandem/métodos
Triazóis/química
[Mh] Termos MeSH secundário: Cucumis sativus/química
Pyrus/química
Estereoisomerismo
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungicides, Industrial); 0 (Soil Pollutants); 0 (Triazoles); 27B9FV58IY (prothioconazole)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171113
[Lr] Data última revisão:
171113
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170829
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02903


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[PMID]:28749955
[Au] Autor:Malandraki I; Beris D; Isaioglou I; Olmos A; Varveri C; Vassilakos N
[Ad] Endereço:Benaki Phytopathological Institute, Department of Phytopathology, Laboratory of Virology, Athens, Greece.
[Ti] Título:Simultaneous detection of three pome fruit tree viruses by one-step multiplex quantitative RT-PCR.
[So] Source:PLoS One;12(7):e0180877, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:A one-step multiplex real-time reverse transcription polymerase chain reaction (RT-qPCR) based on TaqMan probes was developed for the simultaneous detection of Apple mosaic virus (ApMV), Apple stem pitting virus (ASPV) and Apple stem grooving virus (ASGV) in total RNA of pome trees extracted with a CTAB method. The sensitivity of the method was established using in vitro synthesized viral transcripts serially diluted in RNA from healthy, virus-tested (negative) pome trees. The three viruses were simultaneously detected up to a 10-4 dilution of total RNA from a naturally triple-infected apple tree prepared in total RNA of healthy apple tissue. The newly developed RT-qPCR assay was at least one hundred times more sensitive than conventional single RT-PCRs. The assay was validated with 36 field samples for which nine triple and 11 double infections were detected. All viruses were detected simultaneously in composite samples at least up to the ratio of 1:150 triple-infected to healthy pear tissue, suggesting the assay has the capacity to examine rapidly a large number of samples in pome tree certification programs and surveys for virus presence.
[Mh] Termos MeSH primário: Frutas/virologia
Malus/virologia
Vírus de Plantas/fisiologia
Pyrus/virologia
Reação em Cadeia da Polimerase em Tempo Real/métodos
Reação em Cadeia da Polimerase Via Transcriptase Reversa/métodos
Árvores/virologia
[Mh] Termos MeSH secundário: RNA de Plantas/isolamento & purificação
Padrões de Referência
Reprodutibilidade dos Testes
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Plant)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170929
[Lr] Data última revisão:
170929
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170728
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0180877


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[PMID]:28681599
[Au] Autor:Nishiwaki H; Nakazaki S; Akiyama K; Yamauchi S
[Ad] Endereço:Graduate School of Agriculture, Ehime University , 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.
[Ti] Título:Structure-Antifungal Activity Relationship of Fluorinated Dihydroguaiaretic Acid Derivatives and Preventive Activity against Alternaria alternata Japanese Pear Pathotype.
[So] Source:J Agric Food Chem;65(31):6701-6707, 2017 Aug 09.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The structure-activity relationship of the antifungal fluorinated dihydroguaiaretic acid derivatives was evaluated. Some of the newly synthesized lignan compounds were found to show higher antifungal activity against phytopathogenic fungi such as Alternaria alternata (Japanese pear and apple pathotypes) and A. citri than the lead compound, 3-fluoro-3'-methoxylignan-4'-ol (3). The broad antifungal spectrum of 3'-hydroxyphenyl derivative 16 was observed, and the 3'-fluoro-4'-hydroxyphenyl derivative 38 was found to show the highest activity against the A. alternata Japanese pear pathotype, with an EC value of 11 µM. The preventive effect of the potent lignan on the infection of A. alternata in the Japanese pear's leaves was also shown.
[Mh] Termos MeSH primário: Alternaria/efeitos dos fármacos
Fungicidas Industriais/química
Fungicidas Industriais/farmacologia
Guaiacol/análogos & derivados
Lignanas/química
Lignanas/farmacologia
Doenças das Plantas/microbiologia
Pyrus/microbiologia
[Mh] Termos MeSH secundário: Alternaria/crescimento & desenvolvimento
Guaiacol/química
Guaiacol/farmacologia
Doenças das Plantas/prevenção & controle
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fungicides, Industrial); 0 (Lignans); 36469-60-0 (dihydroguaiaretic acid); 6JKA7MAH9C (Guaiacol)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170707
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01896


  9 / 751 MEDLINE  
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[PMID]:28502783
[Au] Autor:Kou X; Qi K; Qiao X; Yin H; Liu X; Zhang S; Wu J
[Ad] Endereço:Centre of Pear Engineering Technology Research, State Key Laboratory of Crop Genetics and Germplasm Enhancement, Nanjing Agricultural University, Nanjing 210095, China. Electronic address: 2014204009@njau.edu.cn.
[Ti] Título:Evolution, expression analysis, and functional verification of Catharanthus roseus RLK1-like kinase (CrRLK1L) family proteins in pear (Pyrus bretchneideri).
[So] Source:Genomics;109(3-4):290-301, 2017 Jul.
[Is] ISSN:1089-8646
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The Catharanthus roseus RLK1-like kinase (CrRLK1L) family is involved in multiple processes during plant growth. However, little is known about CrRLK1L in the wood of the pear fruit tree Pyrus bretchneideri. In this study, 26 CrRLK1L gene members were identified in pear and were grouped into six subfamilies according to phylogenetic analyses. Evolutionary analysis indicated that recent whole genome duplication (WGD) and dispersed gene duplications may contribute to the expansion of the CrRLK1L gene family in pear. Moreover, tissue-specific expression analyses suggested that CrRLK1Ls are involved in the development of various pear tissues. Subsequent qRT-PCR analyses indicated that CrRLK1Ls might play important roles in pollen tube growth. Finally, experiments with antisense oligonucleotides (ASO) demonstrated that PbrCrRLK1L26 have functions in pollen tube elongation and that PbrCrRLK1L3 regulates pollen tube rupture. These results will be useful for elaborating the biological roles of CrRLK1Ls in pear growth and development.
[Mh] Termos MeSH primário: Proteínas de Plantas/fisiologia
Proteínas Quinases/fisiologia
Pyrus/enzimologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Clonagem Molecular
Evolução Molecular
Duplicação Gênica
Perfilação da Expressão Gênica
Regulação da Expressão Gênica de Plantas
Filogenia
Proteínas de Plantas/química
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
Tubo Polínico/crescimento & desenvolvimento
Proteínas Quinases/química
Proteínas Quinases/genética
Proteínas Quinases/metabolismo
Pyrus/genética
Pyrus/crescimento & desenvolvimento
Alinhamento de Sequência
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); EC 2.7.- (Protein Kinases)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170516
[St] Status:MEDLINE


  10 / 751 MEDLINE  
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[PMID]:28421913
[Au] Autor:Tian Y; Zhao Y; Shi L; Cui Z; Hu B; Zhao Y
[Ad] Endereço:First, third, and fifth authors: College of Plant Protection and Key Laboratory of Integrated Management of Crop Diseases and Pests, Ministry of Education, Nanjing Agricultural University, Nanjing 210095, China; second author: Institute of Botany, Jiangsu Province and the Chinese Academy of Sciences
[Ti] Título:Type VI Secretion Systems of Erwinia amylovora Contribute to Bacterial Competition, Virulence, and Exopolysaccharide Production.
[So] Source:Phytopathology;107(6):654-661, 2017 06.
[Is] ISSN:0031-949X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The type VI secretion system (T6SS) plays a major role in mediating interbacterial competition and might contribute to virulence in plant pathogenic bacteria. However, the role of T6SS in Erwinia amylovora remains unknown. In this study, 33 deletion mutants within three T6SS clusters were generated in E. amylovora strain NCPPB1665. Our results showed that all 33 mutants displayed reduced antibacterial activities against Escherichia coli as compared with that of the wild-type (WT) strain, indicating that Erwinia amylovora T6SS are functional. Of the 33 mutants, 19 exhibited reduced virulence on immature pear fruit as compared with that of the WT strain. Among them, 6, 1, and 12 genes belonged to T6SS-1, T6SS-2, and T6SS-3 clusters, respectively. Interestingly, these 19 mutants also produced less amylovoran or levan or both. These findings suggest that E. amylovora T6SS play a role in bacterial competition and virulence possibly by influencing exopolysaccharide production.
[Mh] Termos MeSH primário: Erwinia amylovora
Frutanos/metabolismo
Doenças das Plantas/microbiologia
Polissacarídeos Bacterianos/metabolismo
Pyrus/microbiologia
Sistemas de Secreção Tipo VI/metabolismo
[Mh] Termos MeSH secundário: Erwinia amylovora/genética
Erwinia amylovora/patogenicidade
Erwinia amylovora/fisiologia
Frutas/microbiologia
Deleção de Sequência
Sistemas de Secreção Tipo VI/genética
Virulência
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fructans); 0 (Polysaccharides, Bacterial); 0 (Type VI Secretion Systems); 0 (amylovoran); 9013-95-0 (levan)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170720
[Lr] Data última revisão:
170720
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170420
[St] Status:MEDLINE
[do] DOI:10.1094/PHYTO-11-16-0393-R



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