Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.859.937.500.724 [Categoria DeCS]
Referências encontradas : 105 [refinar]
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  1 / 105 MEDLINE  
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[PMID]:28124036
[Au] Autor:Chen XW; Wang JM; Guo J; Wan ZL; Yin SW; Yang XQ
[Ad] Endereço:Food Protein Research and Development Center, Department of Food Science and Engineering, South China University of Technology, Guangzhou 510640, P. R China. fexqyang@scut.edu.cn fexqyang@163.com.
[Ti] Título:Hierarchical high internal phase emulsions and transparent oleogels stabilized by quillaja saponin-coated nanodroplets for color performance.
[So] Source:Food Funct;8(2):823-831, 2017 Feb 22.
[Is] ISSN:2042-650X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Herein, we report novel high internal phase emulsions and transparent oleogels that exhibit a hierarchical configuration by manipulating the spatial assembly of a natural small molecular-weight quillaja saponin for color performance. Quillaja saponin (QS) is a natural triterpenoid bidesmosidic from the soapbark tree (Quillaja saponaria Molina). Fairly monodispersed QS-coated nanodroplets (∼154 nm) were prepared using the ultrasonic emulsification strategy, and then used as block stabilizers for the fabrication of stable oil-in-water high internal phase emulsions (HIPEs, Ï• = 0.75). The resulting HIPEs can be easily converted into transparent oleogels with a very high oil loading (99.7%) through oven drying (70 °C). The jelly-like oleogels exhibit weak elastic, shear thinning behavior, good thixotropic recovery, and thermostabilization properties, which might be provided by the percolating 3D network of QS fibrils in the oil phase. We spatially tuned the color performance of the HIPEs and subsequent oleogels by locating the compositions of food colorants in different sections of their hierarchal architecture. The design and construction of hierarchical HIPEs and oleogels provide a promising new route for multitask functional delivery applications in various fields including food, cosmetics, and medical applications.
[Mh] Termos MeSH primário: Corantes de Alimentos/química
Extratos Vegetais/química
Saponinas de Quilaia/química
Quillaja/química
[Mh] Termos MeSH secundário: Cor
Emulsões/química
Nanopartículas/química
Compostos Orgânicos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Emulsions); 0 (Food Coloring Agents); 0 (Organic Chemicals); 0 (Plant Extracts); 0 (Quillaja Saponins); 0 (oleogels)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170327
[Lr] Data última revisão:
170327
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170127
[St] Status:MEDLINE
[do] DOI:10.1039/c6fo01752e


  2 / 105 MEDLINE  
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[PMID]:27979218
[Au] Autor:Tippel J; Gies K; Harbaum-Piayda B; Steffen-Heins A; Drusch S
[Ad] Endereço:Department of Food Technology and Food Material Science, Institute of Food Technology and Food Chemistry, Technische Universität Berlin, Königin-Luise-Str. 22, 14195 Berlin, Germany. Electronic address: janine.tippel@campus.tu-berlin.de.
[Ti] Título:Composition of Quillaja saponin extract affects lipid oxidation in oil-in-water emulsions.
[So] Source:Food Chem;221:386-394, 2017 Apr 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Quillaja saponin extract comprises both, surfactants and phenolic compounds, which makes it interesting, in particular, for the formulation of sensitive functional food ingredients and its protection against oxidation. The aim of this study was to investigate the antioxidant effect of Quillaja saponin extract in oil/water emulsions. Emulsions stabilised by Quillaja saponin showed decreased oxidation stability due to naturally occurring metals but stability increased to a great extent when a chelating agent was added. Antioxidant efficiency of the saponin extract was determined photometrically by 2,2'-diphenyl-1-picrylhydrazyl (DPPH) assay and by the use of electron paramagnetic resonance spectroscopy (EPR). EPR spectroscopy applying stable hydrophilic and hydrophobic radicals is advantageous, especially for characterisation of antioxidant efficiency at the interface. The extract showed antioxidant activity towards radicals in both environments, aqueous and hydrophobic, indicating the importance of phenolic compounds for the antioxidant properties of Quillaja saponin extract and their presence at the interface facilitated by saponin molecules.
[Mh] Termos MeSH primário: Emulsões/química
Metabolismo dos Lipídeos/fisiologia
Extratos Vegetais/química
Saponinas de Quilaia/análise
Quillaja/química
[Mh] Termos MeSH secundário: Oxirredução
Fenóis/química
Tensoativos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Emulsions); 0 (Phenols); 0 (Plant Extracts); 0 (Quillaja Saponins); 0 (Surface-Active Agents)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171003
[Lr] Data última revisão:
171003
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161217
[St] Status:MEDLINE


  3 / 105 MEDLINE  
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[PMID]:27760443
[Au] Autor:de Groot C; Müller-Goymann CC
[Ad] Endereço:Institut für Pharmazeutische Technologie, Technische Universität Braunschweig, Braunschweig, Germany.
[Ti] Título:Saponin Interactions with Model Membrane Systems - Langmuir Monolayer Studies, Hemolysis and Formation of ISCOMs.
[So] Source:Planta Med;82(18):1496-1512, 2016 Dec.
[Is] ISSN:1439-0221
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Saponins are used in medicine due to their pharmacological and immunological effects. To better understand interactions of saponins with model membranes and natural membranes of, for example, erythrocytes, Langmuir film balance experiments are well established. For most saponins, a strong interaction with cholesterol was demonstrated in dependence of both the aglycone part and the sugar moieties and is suggested to be correlated with a strong hemolytic activity, high toxicity, and high surface activity, as was demonstrated for the steroid saponin digitonin. In general, changes in the sugar chain or in substituents of the aglycone result in a modification of the saponin properties. A promising saponin with regard to fairly low hemolytic activity and high adjuvant effect is -tomatine, which still shows a high affinity for cholesterol. An interaction with cholesterol and lipids has also been proven for the Quillaja saponin from the bark of Molina. This triterpene saponin was approved in marketed vaccines as an adjuvant due to the formation of immunostimulating complexes. Immunostimulating complexes consist of a Quillaja saponin, cholesterol, phospholipids, and a corresponding antigen. Recently, another saponin from was successfully tested in immunostimulating complexes, too. Based on the results of interaction studies, the formation of drug delivery systems such as immunostimulating complexes or similar self-assembled colloids is postulated for a variety of saponins.
[Mh] Termos MeSH primário: ISCOMs/química
Saponinas/farmacologia
Tomatina/análogos & derivados
[Mh] Termos MeSH secundário: Animais
Células Cultivadas
Hemólise
Membranas Artificiais
Camundongos
Modelos Biológicos
Quillaja/química
Saponinas/química
Tomatina/química
Tomatina/isolamento & purificação
Tomatina/farmacologia
Triterpenos/química
Triterpenos/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (ISCOMs); 0 (Membranes, Artificial); 0 (Saponins); 0 (Triterpenes); 0 (alpha-tomatine); 31U6547O08 (Tomatine)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170428
[Lr] Data última revisão:
170428
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161021
[St] Status:MEDLINE


  4 / 105 MEDLINE  
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[PMID]:27636707
[Au] Autor:Maier C; Reichert CL; Weiss J
[Ad] Endereço:Dept. of Food Physics and Meat Science, Univ. of Hohenheim, Garbenstrasse 21/25, 70599, Stuttgart, Germany.
[Ti] Título:Characterization of Chemically and Thermally Treated Oil-in-Water Heteroaggregates and Comparison to Conventional Emulsions.
[So] Source:J Food Sci;81(10):E2484-E2491, 2016 Oct.
[Is] ISSN:1750-3841
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Heteroaggregated oil-in-water (O/W) emulsions formed by targeted combination of oppositely charged emulsion droplets were proposed to be used for the modulation of physical properties of food systems, ideally achieving the formation of a particulate 3-dimensional network at comparably low-fat content. In this study, rheological properties of Quillaja saponins (QS), sugar beet pectin (SBP), and whey protein isolate (WPI) stabilized conventional and heteroaggregated O/W emulsions at oil contents of 10% to 60% (w/w) were investigated. Selected systems having an oil content of 30% (w/w) and different particle sizes (d ≤ 1.1 or ≥16.7 µm) were additionally subjected to chemical (genipin or glutaraldehyde) and thermal treatments, aiming to increase network stability. Subsequently, their rheological properties and stability were assessed. Yield stresses (τ ) of both conventional and heteroaggregated O/W emulsions were found to depend on emulsifier type, oil content, and initial droplet size. For conventional emulsions, high yield stresses were only observed for SBP-based emulsions (τ , approximately 157 Pa). Highest yield stresses of heteroaggregates were observed when using small droplets stabilized by SBP/WPI (approximately 15.4 Pa), being higher than those of QS/WPI (approximately 1.6 Pa). Subsequent treatments led to significant alterations in rheological properties for SBP/WPI systems, with yield stresses increasing 29-fold (glutaraldehyde) and 2-fold (thermal treatment) compared to untreated heteroaggregates, thereby surpassing yield stresses of similarly treated conventional SBP emulsions. Genipin-driven treatments proved to be ineffective. Results should be of interest to food manufacturers wishing to design viscoelastic food emulsion based systems at lower oil droplet contents.
[Mh] Termos MeSH primário: Emulsões/química
Água/química
Proteínas do Soro do Leite/química
[Mh] Termos MeSH secundário: Beta vulgaris/química
Emulsificantes/química
Análise de Alimentos
Tecnologia de Alimentos
Géis
Glutaral/química
Concentração de Íons de Hidrogênio
Tamanho da Partícula
Quillaja/química
Reologia
Saponinas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Emulsifying Agents); 0 (Emulsions); 0 (Gels); 0 (Saponins); 0 (Whey Proteins); 059QF0KO0R (Water); T3C89M417N (Glutaral)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160917
[St] Status:MEDLINE
[do] DOI:10.1111/1750-3841.13437


  5 / 105 MEDLINE  
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[PMID]:27374503
[Au] Autor:Tippel J; Lehmann M; von Klitzing R; Drusch S
[Ad] Endereço:Department of Food Technology and Food Material Science, Technische Universität Berlin, Königin-Luise-Str. 22, 14195 Berlin, Germany. Electronic address: janine.tippel@campus.tu-berlin.de.
[Ti] Título:Interfacial properties of Quillaja saponins and its use for micellisation of lutein esters.
[So] Source:Food Chem;212:35-42, 2016 Dec 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Natural food colourants, colouring foods and bioactive food ingredients need to be solubilised for their incorporation in food. Aim of the present study was to investigate the micelle-forming properties of saponins from Quillaja saponaria Mollina (QS) in order to solubilise a lutein ester extract for its incorporation in food matrices. QS showed a high surface activity and functionality with respect to micellisation as derived from interfacial tension measurements and subsequent data fitting to the classical Frumkin model. The composition of the aqueous phase affected the lutein ester incorporation as revealed by particle size, zeta potential and colour measurements. In terms of morphology of lutein ester loaded saponin micelles (LMS), cryo-TEM micrographs showed depending on the composition of the medium both, spherical and elongated branched micelles.
[Mh] Termos MeSH primário: Luteína/metabolismo
Micelas
Saponinas de Quilaia/metabolismo
Quillaja/metabolismo
Saponinas/metabolismo
[Mh] Termos MeSH secundário: Ésteres/metabolismo
Tamanho da Partícula
Extratos Vegetais
Tensão Superficial
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Esters); 0 (Micelles); 0 (Plant Extracts); 0 (Quillaja Saponins); 0 (Saponins); X72A60C9MT (Lutein)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170118
[Lr] Data última revisão:
170118
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160705
[St] Status:MEDLINE


  6 / 105 MEDLINE  
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Roehe, Paulo M
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[PMID]:27032516
[Au] Autor:Yendo AC; de Costa F; Cibulski SP; Teixeira TF; Colling LC; Mastrogiovanni M; Soulé S; Roehe PM; Gosmann G; Ferreira FA; Fett-Neto AG
[Ad] Endereço:Plant Physiology Laboratory, Center for Biotechnology and Department of Botany, Federal University of Rio Grande do Sul (UFRGS), Av. Bento Gonçalves, 9500, Porto Alegre, RS 91501-970, Brazil; Faculty of Pharmacy, UFRGS, Av. Ipiranga 2752, Porto Alegre, RS 90610-000, Brazil.
[Ti] Título:A rabies vaccine adjuvanted with saponins from leaves of the soap tree (Quillaja brasiliensis) induces specific immune responses and protects against lethal challenge.
[So] Source:Vaccine;34(20):2305-11, 2016 Apr 29.
[Is] ISSN:1873-2518
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Quillaja brasiliensis (Quillajaceae) is a saponin producing species native from southern Brazil and Uruguay. Its saponins are remarkably similar to those of Q. saponaria, which provides most of the saponins used as immunoadjuvants in vaccines. The immunostimulating capacities of aqueous extract (AE) and purified saponin fraction (QB-90) obtained from leaves of Q. brasiliensis were favorably comparable to those of a commercial saponin-based adjuvant preparation (Quil-A) in experimental vaccines against bovine herpesvirus type 1 and 5, poliovirus and bovine viral diarrhea virus in mice model. Herein, the immunogenicity and protection efficacy of rabies vaccines adjuvanted with Q. brasiliensis AE and its saponin fractions were compared with vaccines adjuvanted with either commercial Quil-A or Alum. Mice were vaccinated with one or two doses (on days 0 and 14) of one of the different vaccines and serum levels of total IgG, IgG1 and IgG2a were quantified over time. A challenge experiment with a lethal dose of rabies virus was carried out with the formulations. Viral RNA detection in the brain of mice was performed by qPCR, and RNA copy-numbers were quantified using a standard curve of in vitro transcribed RNA. All Q. brasiliensis saponin-adjuvanted vaccines significantly enhanced levels of specific IgG isotypes when compared with the no adjuvant group (P ≤ 0.05). Overall, one or two doses of saponin-based vaccine were efficient to protect against the lethal rabies exposure. Both AE and saponin fractions from Q. brasiliensis leaves proved potent immunological adjuvants in vaccines against a lethal challenge with a major livestock pathogen, hence confirming their value as competitive or complementary sustainable alternatives to saponins of Q. saponaria.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/química
Extratos Vegetais/química
Folhas de Planta/química
Saponinas de Quilaia/química
Vacinas Antirrábicas/imunologia
Raiva/prevenção & controle
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/administração & dosagem
Animais
Anticorpos Antivirais/sangue
Encéfalo/virologia
Feminino
Imunoglobulina G/sangue
Camundongos
Extratos Vegetais/administração & dosagem
Quillaja/química
Saponinas de Quilaia/administração & dosagem
RNA Viral/isolamento & purificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antibodies, Viral); 0 (Immunoglobulin G); 0 (Plant Extracts); 0 (Quillaja Saponins); 0 (RNA, Viral); 0 (Rabies Vaccines); 66594-14-7 (Quil A)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160402
[St] Status:MEDLINE


  7 / 105 MEDLINE  
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Roehe, Paulo Michel
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[PMID]:27012913
[Au] Autor:Cibulski SP; Silveira F; Mourglia-Ettlin G; Teixeira TF; dos Santos HF; Yendo AC; de Costa F; Fett-Neto AG; Gosmann G; Roehe PM
[Ad] Endereço:FEPAGRO Saúde Animal, Instituto de Pesquisas Veterinárias Desidério Finamor, Laboratório de Virologia, Eldorado do Sul, RS, Brazil; Departamento de Microbiologia Imunologia e Parasitologia, Laboratório de Virologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
[Ti] Título:Quillaja brasiliensis saponins induce robust humoral and cellular responses in a bovine viral diarrhea virus vaccine in mice.
[So] Source:Comp Immunol Microbiol Infect Dis;45:1-8, 2016 Apr.
[Is] ISSN:1878-1667
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A saponin fraction extracted from Quillaja brasiliensis leaves (QB-90) and a semi-purified aqueous extract (AE) were evaluated as adjuvants in a bovine viral diarrhea virus (BVDV) vaccine in mice. Animals were immunized on days 0 and 14 with antigen plus either QB-90 or AE or an oil-adjuvanted vaccine. Two-weeks after boosting, antibodies were measured by ELISA; cellular immunity was evaluated by DTH, lymphoproliferation, cytokine release and single cell IFN-γ production. Serum anti-BVDV IgG, IgG1 and IgG2b were significantly increased in QB-90- and AE-adjuvanted vaccines. A robust DTH response, increased splenocyte proliferation, Th1-type cytokines and enhanced production of IFN-γ by CD4(+) and CD8(+) T lymphocytes were detected in mice that received QB-90-adjuvanted vaccine. The AE-adjuvanted preparation stimulated humoral responses but not cellular immune responses. These findings reveal that QB-90 is capable of stimulating both cellular and humoral immune responses when used as adjuvant.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos
Anticorpos Antivirais/sangue
Vírus da Diarreia Viral Bovina Tipo 1/imunologia
Imunidade Celular
Imunidade Humoral
Saponinas de Quilaia/imunologia
Vacinas Virais/imunologia
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/administração & dosagem
Animais
Linfócitos T CD4-Positivos/imunologia
Linfócitos T CD8-Positivos/imunologia
Bovinos
Citocinas/secreção
Hipersensibilidade Tardia
Imunoglobulina G/sangue
Interferon gama/imunologia
Ativação Linfocitária
Camundongos
Extratos Vegetais/imunologia
Folhas de Planta/química
Quillaja/química
Saponinas de Quilaia/administração & dosagem
Saponinas de Quilaia/isolamento & purificação
Células Th1/imunologia
Vacinas Virais/administração & dosagem
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Antibodies, Viral); 0 (Cytokines); 0 (Immunoglobulin G); 0 (Plant Extracts); 0 (Quillaja Saponins); 0 (Viral Vaccines); 82115-62-6 (Interferon-gamma)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160326
[St] Status:MEDLINE


  8 / 105 MEDLINE  
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[PMID]:26950114
[Au] Autor:Wang Y; Wang X; Huang J; Li J
[Ad] Endereço:Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China. 15865563261@126.com.
[Ti] Título:Adjuvant Effect of Quillaja saponaria Saponin (QSS) on Protective Efficacy and IgM Generation in Turbot (Scophthalmus maximus) upon Immersion Vaccination.
[So] Source:Int J Mol Sci;17(3):325, 2016 Mar 02.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The adjuvant effect of Quillaja saponaria saponin (QSS) on protection of turbot fry was investigated with immersion vaccination of formalin-killed Vibrio anguillarum O1 and various concentrations of QSS (5, 25, 45 and 65 mg/L). Fish were challenged at days 7, 14 and 28 post-vaccination. Significantly high relative percent of survival (RPS) ((59.1 ± 13.6)%, (81.7 ± 8.2)%, (77.8 ± 9.6)%) were recorded in the fish that received bacterins immersion with QSS at 45 mg/L, which is comparable to the positive control group vaccinated by intraperitoneal injection (IP). Moreover, a remarkably higher serum antibody titer was also demonstrated after 28 days in the vaccinated fish with QSS (45 mg/L) than those vaccinated fish without QSS (p < 0.05), but lower than the IP immunized fish (p < 0.05). Significant upregulation of IgM gene expression has also been identified in the tissues of skin, gill, spleen and kidney from the immunized fish in comparison to the control fish. Taken together, the present study indicated that QSS was able to dramatically evoke systemic and mucosal immune responses in immunized fish. Therefore, QSS might be a promising adjuvant candidate for fish vaccination via an immersion administering route.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/farmacologia
Vacinas Bacterianas/farmacologia
Doenças dos Peixes/prevenção & controle
Linguados/microbiologia
Saponinas/farmacologia
Vibrioses/veterinária
Vibrio/imunologia
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/química
Animais
Vacinas Bacterianas/química
Doenças dos Peixes/imunologia
Doenças dos Peixes/microbiologia
Linguados/imunologia
Imunidade nas Mucosas/efeitos dos fármacos
Imunoglobulina M/imunologia
Quillaja/química
Saponinas/química
Vacinação
Vibrioses/imunologia
Vibrioses/prevenção & controle
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Bacterial Vaccines); 0 (Immunoglobulin M); 0 (Saponins)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160308
[St] Status:MEDLINE


  9 / 105 MEDLINE  
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Roehe, Paulo Michel
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[PMID]:26826546
[Au] Autor:Cibulski SP; Mourglia-Ettlin G; Teixeira TF; Quirici L; Roehe PM; Ferreira F; Silveira F
[Ad] Endereço:FEPAGRO Saúde Animal, Instituto de Pesquisas Veterinárias Desidério Finamor, Laboratório de Virologia, Eldorado do Sul, RS, Brazil; Departamento de Microbiologia Imunologia e Parasitologia, Laboratório de Virologia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil.
[Ti] Título:Novel ISCOMs from Quillaja brasiliensis saponins induce mucosal and systemic antibody production, T-cell responses and improved antigen uptake.
[So] Source:Vaccine;34(9):1162-71, 2016 Feb 24.
[Is] ISSN:1873-2518
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:In the last decades, significant efforts have been dedicated to the search for novel vaccine adjuvants. In this regard, saponins and its formulations as "immunostimulating complexes" (ISCOMs) have shown to be capable of stimulating potent humoral and cellular immune responses, enhanced cytokine production and activation of cytotoxic T cells. The immunological activity of ISCOMs formulated with a saponin fraction extracted from Quillaja brasiliensis (QB-90 fraction) as an alternative to classical ISCOMs based on Quil A(®) (IQA) is presented here. The ISCOMs prepared with QB-90, named IQB-90, typically consist of 40-50 nm, spherical, cage-like particles, built up by QB-90, cholesterol, phospholipids and antigen (ovalbumin, OVA). These nanoparticles were efficiently uptaken in vitro by murine bone marrow-derived dendritic cells. Subcutaneously inoculated IQB-90 induced strong serum antibody responses encompassing specific IgG1 and IgG2a, robust DTH reactions, significant T cell proliferation and increases in Th1 (IFN-γ and IL-2) cytokine responses. Intranasally delivered IQB-90 elicited serum IgG and IgG1, and mucosal IgA responses at distal systemic sites (nasal passages, large intestine and vaginal lumen). These results indicate that IQB-90 is a promising alternative to classic ISCOMs as vaccine adjuvants, capable of enhancing humoral and cellular immunity to levels comparable to those induced by ISCOMs manufactured with Quillaja saponaria saponins.
[Mh] Termos MeSH primário: Adjuvantes Imunológicos/farmacologia
ISCOMs/farmacologia
Imunidade Celular
Imunidade Humoral
Imunidade nas Mucosas
Saponinas de Quilaia/farmacologia
[Mh] Termos MeSH secundário: Adjuvantes Imunológicos/química
Administração Intranasal
Animais
Células da Medula Óssea/imunologia
Cercopithecus aethiops
Citocinas/imunologia
Células Dendríticas/imunologia
Feminino
ISCOMs/química
Imunoglobulina A/imunologia
Imunoglobulina G/sangue
Imunoglobulina G/imunologia
Injeções Subcutâneas
Ativação Linfocitária
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Nanopartículas/química
Quillaja/química
Saponinas de Quilaia/química
Coelhos
Saponinas/química
Saponinas/farmacologia
Linfócitos T Citotóxicos/imunologia
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Adjuvants, Immunologic); 0 (Cytokines); 0 (ISCOMs); 0 (Immunoglobulin A); 0 (Immunoglobulin G); 0 (QB 90U); 0 (Quillaja Saponins); 0 (Saponins); 66594-14-7 (Quil A)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160219
[Lr] Data última revisão:
160219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160131
[St] Status:MEDLINE


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[PMID]:26213058
[Au] Autor:Salminen H; Gömmel C; Leuenberger BH; Weiss J
[Ad] Endereço:Department of Food Physics and Meat Science, Institute of Food Science and Biotechnology, University of Hohenheim, Garbenstrasse 21/25, 70599 Stuttgart, Germany. Electronic address: hanna.salminen01@gmail.com.
[Ti] Título:Influence of encapsulated functional lipids on crystal structure and chemical stability in solid lipid nanoparticles: Towards bioactive-based design of delivery systems.
[So] Source:Food Chem;190:928-937, 2016 Jan 01.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:We investigated the influence of physicochemical properties of encapsulated functional lipids--vitamin A, ß-carotene and ω-3 fish oil--on the structural arrangement of solid lipid nanoparticles (SLN). The relationship between the crystal structure and chemical stability of the incorporated bioactive lipids was evaluated with different emulsifier compositions of a saponin-rich, food-grade Quillaja extract alone or combined with high-melting or low-melting lecithins. The major factors influencing the structural arrangement and chemical stability of functional lipids in solid lipid dispersions were their solubility in the aqueous phase and their crystallization temperature in relation to that of the carrier lipid. The results showed that the stabilization of the α-subcell crystals in the lattice of the carrier lipid is a key parameter for forming stable solid lipid dispersions. This study contributes to a better understanding of SLN as a function of the bioactive lipid.
[Mh] Termos MeSH primário: Sistemas de Liberação de Medicamentos
Lipídeos/química
Nanopartículas/química
[Mh] Termos MeSH secundário: Antioxidantes/farmacologia
Cristalização
Estabilidade de Medicamentos
Ácidos Graxos Ômega-3/química
Lecitinas/administração & dosagem
Transição de Fase
Extratos Vegetais/administração & dosagem
Quillaja
Tensoativos/farmacologia
Vitamina A/química
beta Caroteno/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Fatty Acids, Omega-3); 0 (Lecithins); 0 (Lipids); 0 (Plant Extracts); 0 (Surface-Active Agents); 01YAE03M7J (beta Carotene); 11103-57-4 (Vitamin A)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:170816
[Lr] Data última revisão:
170816
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150728
[St] Status:MEDLINE



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