Base de dados : MEDLINE Pesquisa : B01.650.940.800.575.912.250.875.216 [Categoria DeCS] Referências encontradas : 104 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 28368606 [Au] Autor: Wang YS; Li BT; Liu SX; Wen ZQ; Yang JH; Zhang HB; Hao XJ [Ad] Endereço: Key Laboratory of Medicinal Chemistry for Natural Resource, Ministry of Education, School of Chemical Science and Technology, Yunnan University , Kunming 650091, People's Republic of China. [Ti] Título: Anisucoumaramide, a Bioactive Coumarin from Clausena anisum-olens. [So] Source: J Nat Prod;80(4):798-804, 2017 Apr 28. [Is] ISSN: 1520-6025 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: A new coumarin, anisucoumaramide (1), and a new δ-truxinate derivative, anisumic acid (2), were isolated from Clausena anisum-olens. Their structures were elucidated from extensive NMR and MS data. The absolute configurations of the coumarins were assigned using the experimental and calculated electronic circular dichroism data. Anisucoumaramide (1) represents the first example of a naturally occurring coumarin of which the terpenoidal side chain does not comply with the biosynthesis isoprene rule due to the presence of an unprecedented acetamido motif directly connected with the terpenoidal side chain. The δ-truxinate derivative was isolated from Clausena species for the first time. Compound 1 showed high selectivity for the MAO-B isoenzyme and inhibitory activity in the nanomolar range. Putative biosynthesis pathways toward 1 and 2 are proposed. [Mh] Termos MeSH primário: Clausena/química Cumarínicos/isolamento & purificação Cumarínicos/farmacologia Medicamentos de Ervas Chinesas/isolamento & purificação Medicamentos de Ervas Chinesas/farmacologia [Mh] Termos MeSH secundário: Cumarínicos/química Medicamentos de Ervas Chinesas/química Estrutura Molecular Ressonância Magnética Nuclear Biomolecular [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Coumarins); 0 (Drugs, Chinese Herbal); 0 (anisucoumaramide) [Em] Mês de entrada: 1708 [Cu] Atualização por classe: 170801 [Lr] Data última revisão: 170801 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170404 [St] Status: MEDLINE [do] DOI: 10.1021/acs.jnatprod.6b00391

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[PMID]: 28140741 [Au] Autor: Bautista AM; Tan MA; Apostol JM [Ad] Endereço: a The Graduate School, University of Santo Tomas , Manila , Philippines. [Ti] Título: Lipid-lowering property of Clausena anisum-olens in hypercholesterolemic rats. [So] Source: Pharm Biol;55(1):833-836, 2017 Dec. [Is] ISSN: 1744-5116 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: CONTEXT: Clausena anisum-olens (Blanco) Merr. (Rutaceae) is a medicinal shrub which has been reported to have various pharmacological uses. No study regarding the effects of C. anisum-olens on cholesterol-lowering has been reported. OBJECTIVE: The effects of the ethanol extract of C. anisum-olens leaves on the cholesterol level of hypercholesterolemic rats were evaluated. MATERIALS AND METHODS: Acute oral toxicity of the extract (175, 550 and 2000 mg/kg) was determined using female Sprague-Dawley rats, as described in OECD 425 Main test guidelines. The lipid-lowering assay utilized 30 male Sprague-Dawley rats divided into five groups (A-E). Triton X-100 was administered to induce hypercholesterolemia. After hypercholesterolemia induction, oral treatment of Atorvastatin and crude ethanol extract was given daily to the treatment groups for 14 days. The total cholesterol, triglycerides, HDL and LDL were determined before induction, after induction, after first week of treatment and after second week of treatment. RESULTS: Acute oral toxicity showed the crude extract is nontoxic up to 2000 mg/kg. The lipid-lowering assay indicated reduction of serum cholesterol (87.21 ± 5.10 mg/dL), triglycerides (58.09 ± 4.10 mg/dL) and LDL (27.82 ± 4.11 mg/dL) for 200 mg/kw extract. Reduction in serum cholesterol (74.72 ± 3.64 mg/dL), triglycerides (52.79 ± 2.98 mg/dL) and LDL (12.06 ± 5.51 mg/dL) were observed for 400 mg/kg group. The result is comparable to Atorvastatin, which showed serum cholesterol (80.90 ± 9.72 mg/dL), triglycerides (55.94 ± 7.19 mg/dL) and LDL (22.09 ± 7.60 mg/dL) reduction. DISCUSSION AND CONCLUSION: The crude extract of C. anisum-olens proved to be useful in lowering of cholesterol. [Mh] Termos MeSH primário: Anticolesterolemiantes/farmacologia Clausena Hipercolesterolemia/tratamento farmacológico Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Animais Feminino Masculino Extratos Vegetais/toxicidade Ratos Ratos Sprague-Dawley [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anticholesteremic Agents); 0 (Plant Extracts) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 170306 [Lr] Data última revisão: 170306 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170201 [St] Status: MEDLINE [do] DOI: 10.1080/13880209.2016.1260596

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[PMID]: 28128839 [Au] Autor: Chai WM; Lin MZ; Feng HL; Zou ZR; Wang YX [Ad] Endereço: College of Life Science and Key Laboratory of Poyang Lake Wetland and Watershed Research, Ministry of Education, Jiangxi Normal University, Nanchang, Jiangxi 330022, China. chaiweiming@jxnu.edu.cn zouzhr@163.com and Key Laboratory of Small Functional Organic Molecule, Ministry of Education, Jiangxi [Ti] Título: Proanthocyanidins purified from fruit pericarp of Clausena lansium (Lour.) Skeels as efficient tyrosinase inhibitors: structure evaluation, inhibitory activity and molecular mechanism. [So] Source: Food Funct;8(3):1043-1051, 2017 Mar 22. [Is] ISSN: 2042-650X [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Fruit pericarp of Clausena lansium (Lour.) Skeels, a food waste, was selected as a raw material for proanthocyanidins. The proanthocyanidins' structures were integrally analyzed using three methods: matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), high performance liquid chromatography electrospray ionization mass spectrometry (HPLC-ESI-MS) and C nuclear magnetic resonance (NMR). The results elucidated that these compounds were composed of prodelphinidin (75%) and procyanidin (25%) with a degree of polymerization (DP) up to the 20-mers. They were proved to be remarkable, reversible and mixed competitive inhibitors of tyrosinase according to results from enzyme experiments. The IC values were calculated to be 23.6 ± 1.2 and 7.0 ± 0.2 µg mL for the monophenolase and diphenolase activities, respectively. In addition, the proanthocyanidins had a good inhibitory effect on cell proliferation, cellular tyrosinase activity and melanin production of B mouse melanoma cells. Chelation between the hydroxyl group on the B ring of the proanthocyanidins and dicopper irons of the enzyme provided one of the feasible mechanisms for the inhibition on the basis of fluorescence quenching and molecular docking analyses. This research would supply the scientific basis to these compounds application in the pharmaceutical, insecticides, and preservative fields. [Mh] Termos MeSH primário: Clausena/química Inibidores Enzimáticos/química Frutas/química Monofenol Mono-Oxigenase/antagonistas & inibidores Extratos Vegetais/química Proantocianidinas/química [Mh] Termos MeSH secundário: Animais Linhagem Celular Sobrevivência Celular Células/efeitos dos fármacos Células/enzimologia Cromatografia Líquida de Alta Pressão Inibidores Enzimáticos/isolamento & purificação Inibidores Enzimáticos/farmacologia Cinética Camundongos Estrutura Molecular Monofenol Mono-Oxigenase/química Monofenol Mono-Oxigenase/metabolismo Extratos Vegetais/isolamento & purificação Extratos Vegetais/farmacologia Proantocianidinas/isolamento & purificação Proantocianidinas/farmacologia Espectrometria de Massas por Ionização por Electrospray [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Enzyme Inhibitors); 0 (Plant Extracts); 0 (Proanthocyanidins); EC 1.14.18.1 (Monophenol Monooxygenase) [Em] Mês de entrada: 1707 [Cu] Atualização por classe: 170713 [Lr] Data última revisão: 170713 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170128 [St] Status: MEDLINE [do] DOI: 10.1039/c6fo01320a

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[PMID]: 27539584 [Au] Autor: Shen DY; Kuo PC; Huang SC; Hwang TL; Chan YY; Shieh PC; Ngan NT; Thang TD; Wu TS [Ad] Endereço: School of Pharmacy, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, 701, Taiwan, ROC. [Ti] Título: Constituents from the leaves of Clausena lansium and their anti-inflammatory activity. [So] Source: J Nat Med;71(1):96-104, 2017 Jan. [Is] ISSN: 1861-0293 [Cp] País de publicação: Japan [La] Idioma: eng [Ab] Resumo: Five new acyclic amides, clausenalansamides C-G (1-5), clausenaline G (6) and (±)-5-(4-methylphenyl)-γ-valerolactone (7) reported from the natural source for the first time, were characterized from the leaves of Clausena lansium. Their structures were established by spectroscopic and spectrometric methods, and the absolute configurations of new compounds 1-5 were determined by electronic circular dichroism and single-crystal X-ray diffraction analyses. Eighteen compounds were evaluated for their anti-inflammatory activity and only imperatorin (11) and wampetin (12) displayed significant inhibition of fMLP/CB-induced superoxide anion generation with IC values of 1.7 ± 0.3 and 6.8 ± 1.1 µM, respectively. [Mh] Termos MeSH primário: Anti-Inflamatórios/química Clausena/química Folhas de Planta/química [Mh] Termos MeSH secundário: Anti-Inflamatórios/farmacologia Estrutura Molecular [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Inflammatory Agents) [Em] Mês de entrada: 1703 [Cu] Atualização por classe: 171104 [Lr] Data última revisão: 171104 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160820 [St] Status: MEDLINE [do] DOI: 10.1007/s11418-016-1033-x

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[PMID]: 27836776 [Au] Autor: Mukandiwa L; Naidoo V; Katerere DR [Ad] Endereço: Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, P. Bag X04, Ondesterpoort 0110, South Africa. [Ti] Título: The use of Clausena anisata in insect pest control in Africa: A review. [So] Source: J Ethnopharmacol;194:1103-1111, 2016 Dec 24. [Is] ISSN: 1872-7573 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: ETHNOPHARMACOLOGICAL RELEVANCE: Clausena anisata is used traditionally by various communities across Africa against pests such as mosquitoes, flies and weevils among others. Pests are a major cause of disease and production losses in various crop and livestock production systems in Africa. This review discusses the available information on the occurrence, chemistry, biological activity and possible commercialization of Clausena anisata with a view to see the plant species being integrated in pest management. MATERIALS AND METHODS: Information on the ethnomedical use, chemistry and biological activity of C. anisata published between 1980 and 2016 was accessed from various databases namely Science Direct, Springer Link and Wiley Online Library. In addition various relevant books were also consulted. RESULTS: The crude extracts as well as different fractions of C. anisata have been evaluated for activity against various insect pests and have been shown to be active. Furthermore, close to 50 compounds have been isolated and identified from C. anisata, which include coumarins, carbazole alkaloids, limonoids and essential oils (monoterpenes). Some of these compounds have been proven to exhibit pesticidal properties in both laboratory and field studies against various pests including mosquitoes, flies and weevils. The possible mechanisms of action of these compounds have been explored in this review. CONCLUSION: The results of pesticidal and phytochemical screening of C. anisata strongly indicate that the species is endowed with pesticidal properties that can be harnessed into commercial products. However, one glaring challenge in the evaluation of this plant species for pesticidal activity has been the non-availability of standard testing systems. Researchers have used various methods which they developed based on their own circumstances and resources. Formulation, standard appropriate testing systems and agronomic research are key in unlocking the potential of this important African species. [Mh] Termos MeSH primário: Clausena/química Insetos/efeitos dos fármacos Controle de Pragas/métodos Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: África Animais Seres Humanos Extratos Vegetais/química [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: 0 (Plant Extracts) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161113 [St] Status: MEDLINE

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[PMID]: 27796113 [Au] Autor: Mukandiwa L; Eloff JN; Sibanda DR; Naidoo V [Ad] Endereço: Department of Paraclinical Sciences, University of Pretoria. Lilian.Mukandiwa@up.ac.za. [Ti] Título: An acetone extract of Clausena anisata may be a potential control agent for flies encountered in cutaneous myiasis. [So] Source: Onderstepoort J Vet Res;83(1):e1-e7, 2016 May 24. [Is] ISSN: 2219-0635 [Cp] País de publicação: South Africa [La] Idioma: eng [Ab] Resumo: Clausena anisata is a medicinal plant used traditionally to treat myiasis and as an insect repellent by various communities. We have previously demonstrated the effects of C. anisata extracts on blowfly feeding and development in our laboratory. The impact of C. anisata leaf extracts on populations of different fly species on farms in Mpumalanga, South Africa was investigated in this study under field conditions. Flies were exposed to liver baits treated with acetone leaf extracts of C. anisata (150 mg/mL). Fly numbers and composition on two farms, with and without C. anisata treated liver, were compared during a period of 12 weeks when fly populations were expected to be high. Observations were made on fly behaviour and development, adult sizes and numbers. The flies exposed to liver treated with the leaf extract of C. anisata had a decreased rate of development, prolonged larval period, smaller body sizes and more sluggish behaviour compared to those subjected to the control treatment. No significant differences were, however, found between the numbers and sizes of flies on the treated and on the control farm, which was most likely due to the limited nature of the baiting programme we followed. The effects of C. anisata extracts on blowfly behaviour and development observed in previous laboratory studies were confirmed in this field evaluation. Although the extracts did not have a significant effect on the overall population size in this experiment, we believe that the C. anisata leaf extract could be useful in integrated pest management based on its effect on larval development. In addition, species such as Lucilia cuprina and Chrysomya marginalis seemed to have been repelled by the C. anisata treated liver; as a result, further work should explore this aspect and how it can be used for the protection of animals. [Mh] Termos MeSH primário: Doenças dos Bovinos/prevenção & controle Clausena Dípteros/efeitos dos fármacos Repelentes de Insetos/farmacologia Miíase/veterinária Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Animais Bovinos Controle de Insetos Miíase/prevenção & controle Fitoterapia/veterinária África do Sul [Pt] Tipo de publicação: JOURNAL ARTICLE; MULTICENTER STUDY [Nm] Nome de substância: 0 (Insect Repellents); 0 (Plant Extracts) [Em] Mês de entrada: 1702 [Cu] Atualização por classe: 170206 [Lr] Data última revisão: 170206 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161101 [St] Status: MEDLINE [do] DOI: 10.4102/ojvr.v83i1.1045

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[PMID]: 27763535 [Au] Autor: Ashwaq AS; Al-Qubaisi MS; Rasedee A; Abdul AB; Taufiq-Yap YH; Yeap SK [Ad] Endereço: MAKNA-UPM, Cancer Research Laboratory, Institute of Bioscience, University Putra Malaysia, 43400 Selangor, Malaysia. ashwaq.shaker@yahoo.com. [Ti] Título: Inducing G2/M Cell Cycle Arrest and Apoptosis through Generation Reactive Oxygen Species (ROS)-Mediated Mitochondria Pathway in HT-29 Cells by Dentatin (DEN) and Dentatin Incorporated in Hydroxypropyl-ß-Cyclodextrin (DEN-HPßCD). [So] Source: Int J Mol Sci;17(10), 2016 Oct 18. [Is] ISSN: 1422-0067 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: Dentatin (DEN), purified from the roots of Burm f., has poor aqueous solubility that reduces its therapeutic application. The aim of this study was to assess the effects of DEN-HPßCD (hydroxypropyl-ß-cyclodextrin) complex as an anticancer agent in HT29 cancer cell line and compare with a crystal DEN in dimethyl sulfoxide (DMSO). The exposure of the cancer cells to DEN or DEN-HPßCD complex leads to cell growth inhibition as determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. To analyze the mechanism, in which DEN or DEN-HPßCD complex causes the death in human colon HT29 cancer cells, was evaluated by the enzyme-linked immunosorbent assay (ELIZA)-based assays for caspase-3, 8, 9, and reactive oxygen species (ROS). The findings showed that an anti-proliferative effect of DEN or DEN-HPßCD complex were via cell cycle arrest at the G2/M phase and eventually induced apoptosis through both mitochondrial and extrinsic pathways. The down-regulation of poly(ADP-ribose) polymerase (PARP) which leaded to apoptosis upon treatment, was investigated by Western-blotting. Hence, complexation between DEN and HPßCD did not diminish or eliminate the effective properties of DEN as anticancer agent. Therefore, it would be possible to resolve the conventional and current issues associated with the development and commercialization of antineoplastic agents in the future. [Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia Apoptose/efeitos dos fármacos Neoplasias do Colo/tratamento farmacológico Pontos de Checagem da Fase G2 do Ciclo Celular/efeitos dos fármacos Compostos Heterocíclicos com 3 Anéis/farmacologia Pontos de Checagem da Fase M do Ciclo Celular/efeitos dos fármacos Espécies Reativas de Oxigênio/metabolismo [Mh] Termos MeSH secundário: 2-Hidroxipropil-beta-Ciclodextrina Antineoplásicos Fitogênicos/administração & dosagem Clausena/química Neoplasias do Colo/metabolismo Neoplasias do Colo/patologia Portadores de Fármacos/química Células HT29 Compostos Heterocíclicos com 3 Anéis/administração & dosagem Seres Humanos Mitocôndrias/efeitos dos fármacos Mitocôndrias/metabolismo Mitocôndrias/patologia Transdução de Sinais/efeitos dos fármacos beta-Ciclodextrinas/química [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Drug Carriers); 0 (Heterocyclic Compounds, 3-Ring); 0 (Reactive Oxygen Species); 0 (beta-Cyclodextrins); 0 (dentatin); 1I96OHX6EK (2-Hydroxypropyl-beta-cyclodextrin) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 171116 [Lr] Data última revisão: 171116 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161021 [St] Status: MEDLINE

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[PMID]: 27729282 [Au] Autor: Waziri PM; Abdullah R; Yeap SK; Omar AR; Abdul AB; Kassim NK; Malami I; Karunakaran T; Imam MU [Ad] Endereço: MAKNA Cancer Research Laboratory, Institute of Bioscience, University Putra Malaysia, Serdang, Selangor, Malaysia; Department of Biochemistry, Kaduna State University, Main Campus, PMB 2336 Kaduna, Nigeria. Electronic address: petermwaziri@gmail.com. [Ti] Título: Clausenidin from Clausena excavata induces apoptosis in hepG2 cells via the mitochondrial pathway. [So] Source: J Ethnopharmacol;194:549-558, 2016 Dec 24. [Is] ISSN: 1872-7573 [Cp] País de publicação: Ireland [La] Idioma: eng [Ab] Resumo: ETHNOPHARMACOLOGICAL RELEVANCE: Clausena excavata Burm.f. is used locally in folk medicine for the treatment of cancer in South East Asia. AIM OF THE STUDY: To determine the mechanism of action of pure clausenidin crystals in the induction of hepatocellular carcinoma (hepG2) cells apoptosis. MATERIALS AND METHODS: Pure clausenidin was isolated from Clausena excavata Burm.f. and characterized using H and C NMR spectra. Clausenidin-induced cytotoxicity was determined by MTT assay. The morphology of hepG2 after treatment with clausenidin was determined by fluorescence and Scanning Electron Microscopy. The effect of clausenidin on the apoptotic genes and proteins were determined by real-time qPCR and protein array profiling, respectively. The involvement of the mitochondria in clausenidin-induced apoptosis was investigated using MMP, caspase 3 and 9 assays. RESULTS: Clausenidin induced significant (p<0.05) and dose-dependent apoptosis of hepG2 cells. Cell cycle assay showed that clausenidin induced a G2/M phase arrest, caused mitochondrial membrane depolarization and significantly (p<0.05) increased expression of caspases 3 and 9, which suggest the involvement of the mitochondria in the apoptotic signals. In addition, clausenidin caused decreased expression of the anti-apoptotic protein, Bcl 2 and increased expression of the pro-apoptotic protein, Bax. This finding was confirmed by the downregulation of Bcl-2 gene and upregulation of the Bax gene in the treated hepG2 cells. CONCLUSION: Clausenidin extracted from Clausena excavata Burm.f. is an anti-hepG2 cell compound as shown by its ability to induce apoptosis through the mitochondrial pathway of apoptosis. Clausenidin can potentially be developed into an anticancer compound. [Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos Clausena/química Mitocôndrias/efeitos dos fármacos Extratos Vegetais/farmacologia [Mh] Termos MeSH secundário: Caspase 3/metabolismo Caspase 9/metabolismo Expressão Gênica Células Hep G2 Seres Humanos Potencial da Membrana Mitocondrial/efeitos dos fármacos Microscopia Eletrônica de Varredura Mitocôndrias/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Plant Extracts); EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 9) [Em] Mês de entrada: 1704 [Cu] Atualização por classe: 170421 [Lr] Data última revisão: 170421 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 161013 [St] Status: MEDLINE

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[PMID]: 27473055 [Au] Autor: Waziri PM; Abdullah R; Yeap SK; Omar AR; Kassim NK; Malami I; How CW; Etti IC; Abu ML [Ad] Endereço: MAKNA Cancer Research Laboratory, Institute of Bioscience, University Putra Malaysia, Serdang, Selangor, Malaysia. petermwaziri@gmail.com. [Ti] Título: Clausenidin induces caspase-dependent apoptosis in colon cancer. [So] Source: BMC Complement Altern Med;16:256, 2016 Jul 29. [Is] ISSN: 1472-6882 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Clausena excavata Burm.f. is a shrub traditionally used to treat cancer patients in Asia. The main bioactive chemical components of the plant are alkaloids and coumarins. In this study, we isolated clausenidin from the roots of C. excavata to determine its apoptotic effect on the colon cancer (HT-29) cell line. METHOD: We examined the effect of clausenidin on cell viability, ROS generation, DNA fragmentation, mitochondrial membrane potential in HT-29 cells. Ultrastructural analysis was conducted for morphological evidence of apoptosis in the treated HT-29 cells. In addition, we also evaluated the effect of clausenidin treatment on the expression of caspase 3 and 9 genes and proteins in HT-29 cells. RESULT: Clausenidin induced a G0/G1 cell cycle arrest in HT-29 cells with significant (p < 0.05) dose-dependent increase in apoptotic cell population. The DNA fragmentation assay also showed apoptotic features in the clausenidin-treated HT-29 cells. Clausenidin treatment had caused significant (p < 0.05) increases in the expression of caspase 9 protein and gene in HT-29 cells and mitochondrial ROS and mitochondrial membrane depolarization. The results suggest the involvement of the mitochondria in the caspase-dependent apoptosis in clausenidin-treated colon cancer cells. CONCLUSION: Clausenidin induces a caspase-dependent apoptosis in colon cancers through the stimulation of the mitochondria. The study demonstrates the potential of clausenidin for use in the treatment of colon cancers. [Mh] Termos MeSH primário: Apoptose/efeitos dos fármacos Caspases/metabolismo Clausena/química Neoplasias do Colo/metabolismo Extratos Vegetais/farmacologia Piranocumarinas/farmacologia [Mh] Termos MeSH secundário: Ciclo Celular/efeitos dos fármacos Sobrevivência Celular/efeitos dos fármacos Fragmentação do DNA/efeitos dos fármacos Expressão Gênica/efeitos dos fármacos Células HT29 Seres Humanos Extratos Vegetais/química Piranocumarinas/química Espécies Reativas de Oxigênio/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Plant Extracts); 0 (Pyranocoumarins); 0 (Reactive Oxygen Species); EC 3.4.22.- (Caspases) [Em] Mês de entrada: 1702 [Cu] Atualização por classe: 170220 [Lr] Data última revisão: 170220 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160731 [St] Status: MEDLINE [do] DOI: 10.1186/s12906-016-1247-1

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[PMID]: 27450780 [Au] Autor: Ouyang GQ; Li CJ; Yang JZ; Li L; Song XY; Jiang YN; Chen NH; Ma J; Zhang DM [Ad] Endereço: State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, 100050, P. R. China. [Ti] Título: Bioactive Coumarins from the Stems of Clausena emarginata. [So] Source: Chem Biodivers;13(9):1178-1185, 2016 Sep. [Is] ISSN: 1612-1880 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: Five new coumarins, clauemarmarins I - M (1 - 4), together with 10 known analogs (5 - 14), were isolated from the stems of Clausena emarginata. Compounds 8 - 13 were obtained from this plant for the first time. Their structures were established and elucidated by comprehensive analysis of spectroscopic data. The absolute configurations of 1 - 4 were further determined by their electronic circular dichroism spectroscopy. Compounds 5, 7, 12, and 14 exhibited inhibitory effects on LPS-induced NO production. Compounds 5 - 7 showed selective neuroprotective effects in Aß model at 10 µm. [Mh] Termos MeSH primário: Clausena/química Cumarínicos/isolamento & purificação Cumarínicos/farmacologia Fármacos Neuroprotetores/isolamento & purificação Fármacos Neuroprotetores/farmacologia Caules de Planta/química [Mh] Termos MeSH secundário: Peptídeos beta-Amiloides/antagonistas & inibidores Peptídeos beta-Amiloides/metabolismo Animais Linhagem Celular Sobrevivência Celular/efeitos dos fármacos Cumarínicos/química Relação Dose-Resposta a Droga Seres Humanos Lipopolissacarídeos/antagonistas & inibidores Lipopolissacarídeos/farmacologia Estrutura Molecular Fármacos Neuroprotetores/química Óxido Nítrico/antagonistas & inibidores Óxido Nítrico/biossíntese Células PC12 Fragmentos de Peptídeos/antagonistas & inibidores Fragmentos de Peptídeos/metabolismo Ratos Relação Estrutura-Atividade [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Amyloid beta-Peptides); 0 (Coumarins); 0 (Lipopolysaccharides); 0 (Neuroprotective Agents); 0 (Peptide Fragments); 0 (amyloid beta-protein (25-35)); 31C4KY9ESH (Nitric Oxide) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170118 [Lr] Data última revisão: 170118 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160725 [St] Status: MEDLINE [do] DOI: 10.1002/cbdv.201500519

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