Base de dados : MEDLINE Pesquisa : B01.650.940.800.575.912.250.875.717 [Categoria DeCS] Referências encontradas : 156 [refinar] Mostrando: 1 .. 10   no formato [Detalhado]

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[PMID]: 28425165 [Au] Autor: Akihisa T; Yokokawa S; Ogihara E; Matsumoto M; Zhang J; Kikuchi T; Koike K; Abe M [Ad] Endereço: Research Institute for Science & Technology, Tokyo University of Science, 2641 Yamazaki, Noda, Chiba, 278-8510, Japan. [Ti] Título: Melanogenesis-Inhibitory and Cytotoxic Activities of Limonoids, Alkaloids, and Phenolic Compounds from Phellodendron amurense Bark. [So] Source: Chem Biodivers;14(7), 2017 Jul. [Is] ISSN: 1612-1880 [Cp] País de publicação: Switzerland [La] Idioma: eng [Ab] Resumo: Four limonoids, 1 - 4, five alkaloids, 5 - 9, and four phenolic compounds, 10 - 13, were isolated from a MeOH extract of the bark of Phellodendron amurense (Rutaceae). Among these, compound 13 was new, and its structure was established as rel-(1R,2R,3R)-5-hydroxy-3-(4-hydroxy-3-methoxyphenyl)-6-methoxy-1-(methoxycarbonylmethyl)indane-2-carboxylic acid methyl ester (γ-di(methyl ferulate)) based on the spectrometric analysis. Upon evaluation of compounds 1 - 13 against the melanogenesis in the B16 melanoma cells induced with α-melanocyte-stimulating hormone (α-MSH), four compounds, limonin (1), noroxyhydrastinine (6), haplopine (7), and 4-methoxy-1-methylquinolin-2(1H)-one (8), exhibited potent melanogenesis-inhibitory activities with almost no toxicity to the cells. Western blot analysis revealed that compound 6 inhibited melanogenesis, at least in part, by inhibiting the expression of protein levels of tyrosinase, TRP-1, and TRP-2 in α-MSH-stimulated B16 melanoma cells. In addition, when compounds 1 - 13 were evaluated for their cytotoxic activities against leukemia (HL60), lung (A549), duodenum (AZ521), and breast (SK-BR-3) cancer cell lines, five compounds, berberine (5), 8, canthin-6-one (9), α-di-(methyl ferulate) (12), and 13, exhibited cytotoxicities against one or more cancer cell lines with IC values in the range of 2.6 - 90.0 µm. In particular, compound 5 exhibited strong cytotoxicity against AZ521 (IC 2.6 µm) which was superior to that of the reference cisplatin (IC 9.5 µm). [Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/isolamento & purificação Phellodendron/química [Mh] Termos MeSH secundário: Alcaloides/isolamento & purificação Alcaloides/farmacologia Antineoplásicos Fitogênicos/química Antineoplásicos Fitogênicos/farmacologia Carcinogênese Linhagem Celular Tumoral Seres Humanos Concentração Inibidora 50 Limoninas/isolamento & purificação Limoninas/farmacologia Melanoma Experimental/patologia Metanol Fenóis/isolamento & purificação Fenóis/farmacologia Casca de Planta/química Extratos Vegetais [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Alkaloids); 0 (Antineoplastic Agents, Phytogenic); 0 (Limonins); 0 (Phenols); 0 (Plant Extracts); Y4S76JWI15 (Methanol) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170922 [Lr] Data última revisão: 170922 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170421 [St] Status: MEDLINE [do] DOI: 10.1002/cbdv.201700105

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[PMID]: 27648555 [Au] Autor: Wang L; Wang X; Zhu XM; Liu YQ; Du WJ; Ji J; He X; Zhang CF; Li F; Guo CR; Wang CZ; Yuan CS [Ad] Endereço: State Key Laboratory of Natural Medicines, China Pharmaceutical University, Nanjing, China. [Ti] Título: Gastroprotective Effect of Alkaloids from Cortex Phellodendri on Gastric Ulcers in Rats through Neurohumoral Regulation. [So] Source: Planta Med;83(3-04):277-284, 2017 Feb. [Is] ISSN: 1439-0221 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: The present study aimed to investigate the gastroprotective activity of the total alkaloids from the bark of and identify their possible mechanism. Total alkaloids were obtained through an alcohol extraction method and were analyzed using LC-MS/MS. Chronic gastric ulcers were induced by acetic acid (0.14 mol/L) filter paper on the gastric serosa. The antiulcer effect of total alkaloids was evaluated using the ulcer area, the ulcer inhibition ratio, and epidermal growth factor. The gastroprotective mechanism of total alkaloids was revealed using the levels of serotonin and noradrenaline. The results showed that oral administration of total alkaloids (30 mg/kg/day) obviously decreased the ulcer area (7.67 ± 2.06 mm ; p < 0.01) compared with the model group (15.15 ± 2.34 mm ). The ulcer inhibition ratio of the total alkaloids group (50 %) was higher than the omeprazole-treated group (46 %), which showed that the antiulcer effect of the total alkaloids may be superior to omeprazole. Besides, the total alkaloids significantly increased the epidermal growth factor level and accelerated the healing of ulcers. Histological examination of gastric tissues also supported the same conclusion. In addition, the total alkaloids significantly elevated the levels of serotonin and noradrenaline (both p < 0.01 compared to the model group). Our data indicates that total alkaloids of Cortex Phellodendri exerts a beneficial gastroprotective effect and the involved mechanism is likely neurohumoral regulation. Thus, Cortex Phellodendri may develop into a promising clinical medicinal agent for improving the quality of ulcer healing. [Mh] Termos MeSH primário: Alcaloides/farmacologia Antiulcerosos/farmacologia Neurotransmissores/metabolismo Phellodendron/química Extratos Vegetais/farmacologia Úlcera Gástrica/tratamento farmacológico [Mh] Termos MeSH secundário: Ácido Acético/efeitos adversos Alcaloides/química Alcaloides/isolamento & purificação Animais Antiulcerosos/química Carboximetilcelulose Sódica/farmacologia Fator de Crescimento Epidérmico/sangue Mucosa Gástrica/efeitos dos fármacos Mucosa Gástrica/metabolismo Mucosa Gástrica/patologia Masculino Omeprazol/farmacologia Extratos Vegetais/química Ratos Ratos Sprague-Dawley Rutaceae/química Úlcera Gástrica/metabolismo Úlcera Gástrica/patologia [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Alkaloids); 0 (Anti-Ulcer Agents); 0 (Neurotransmitter Agents); 0 (Plant Extracts); 62229-50-9 (Epidermal Growth Factor); K679OBS311 (Carboxymethylcellulose Sodium); KG60484QX9 (Omeprazole); Q40Q9N063P (Acetic Acid) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170621 [Lr] Data última revisão: 170621 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160921 [St] Status: MEDLINE [do] DOI: 10.1055/s-0042-114044

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[PMID]: 27450803 [Au] Autor: Jiang T; Qian J; Ding J; Wang G; Ding X; Liu S; Chen W [Ad] Endereço: Institution for Laboratory Medicine, Changshu, Jiangsu, 215500, China. [Ti] Título: Metabolomic profiles delineate the effect of Sanmiao wan on hyperuricemia in rats. [So] Source: Biomed Chromatogr;31(2), 2017 Feb. [Is] ISSN: 1099-0801 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: A serum metabolomic method based on ultra-high-performance liquid chromatography coupled with mass spectrometry was developed to characterize hyperuricemia-related metabolic profiles and delineate the mechanism of Sanmiao wan (SMW), a traditional Chinese medicine (TCM), in treating hyperuricemic rats. With partial least-squares discriminant analysis for classification and selection of biomarkers, 13 potential biomarkers in mouse serum were identified in the screen, primarily involved in purine metabolism, arginine and proline metabolism, citrate cycle, phenylalanine metabolism, tryptophan metabolism and glycerophospholipid metabolism. Taking these potential biomarkers as screening indexes, SMW could reverse the pathological process of hyperuricemia through partially regulating the perturbed metabolic pathway except for glycerophospholipid metabolism. Our results showed that a metabolomic approach offers a useful tool to identify hyperuricemia-related biomarkers and provides a new methodological cue for systematically dissecting the underlying efficacies and mechanisms of TCM in treating hyperuricemia. [Mh] Termos MeSH primário: Medicamentos de Ervas Chinesas/uso terapêutico Hiperuricemia/tratamento farmacológico Metaboloma/efeitos dos fármacos [Mh] Termos MeSH secundário: Achyranthes/química Animais Atractylodes/química Biomarcadores/sangue Biomarcadores/metabolismo Cromatografia Líquida de Alta Pressão/métodos Medicamentos de Ervas Chinesas/química Medicamentos de Ervas Chinesas/farmacologia Glicerofosfolipídeos/metabolismo Hiperuricemia/sangue Hiperuricemia/metabolismo Análise dos Mínimos Quadrados Masculino Redes e Vias Metabólicas/efeitos dos fármacos Metabolômica/métodos Camundongos Phellodendron/química Ratos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Biomarkers); 0 (Drugs, Chinese Herbal); 0 (Glycerophospholipids) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170131 [Lr] Data última revisão: 170131 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160725 [St] Status: MEDLINE [do] DOI: 10.1002/bmc.3792

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[PMID]: 27208550 [Au] Autor: Gong J; Muñoz AR; Pingali S; Payton-Stewart F; Chan DE; Freeman JW; Ghosh R; Kumar AP [Ad] Endereço: Department of Pharmacology, University of Texas Health Science Center, San Antonio, Texas. [Ti] Título: Downregulation of STAT3/NF-κB potentiates gemcitabine activity in pancreatic cancer cells. [So] Source: Mol Carcinog;56(2):402-411, 2017 Feb. [Is] ISSN: 1098-2744 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: There is an unmet need to develop new agents or strategies against therapy resistant pancreatic cancer (PanCA). Recent studies from our laboratory showed that STAT3 negatively regulates NF-κB and that inhibition of this crosstalk using Nexrutine® (Nx) reduces transcriptional activity of COX-2. Inhibition of these molecular interactions impedes pancreatic cancer cell growth as well as reduces fibrosis in a preclinical animal model. Nx is an extract derived from the bark of Phellodendron amurense and has been utilized in traditional Chinese medicine as antidiarrheal, astringent, and anti-inflammatory agent for centuries. We hypothesized that "Nx-mediated inhibition of survival molecules like STAT3 and NF-κB in pancreatic cancer cells will improve the efficacy of the conventional chemotherapeutic agent, gemcitabine (GEM)." Therefore, we explored the utility of Nx, one of its active constituents berberine and its derivatives, to enhance the effects of GEM. Using multiple human pancreatic cancer cells we found that combination treatment with Nx and GEM resulted in significant alterations of proteins in the STAT3/NF-κB signaling axis culminating in growth inhibition in a synergistic manner. Furthermore, GEM resistant cells were more sensitive to Nx treatment than their parental GEM-sensitive cells. Interestingly, although berberine, the Nx active component used, and its derivatives were biologically active in GEM sensitive cells they did not potentiate GEM activity when used in combination. Taken together, these results suggest that the natural extract, Nx, but not its active component, berberine, has the potential to improve GEM sensitivity, perhaps by down regulating STAT3/NF-κB signaling. © 2016 Wiley Periodicals, Inc. [Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia Antimetabólitos Antineoplásicos/farmacologia Desoxicitidina/análogos & derivados Resistência a Medicamentos Antineoplásicos/efeitos dos fármacos NF-kappa B/antagonistas & inibidores Neoplasias Pancreáticas/tratamento farmacológico Extratos Vegetais/farmacologia Fator de Transcrição STAT3/antagonistas & inibidores [Mh] Termos MeSH secundário: Anti-Inflamatórios/química Berberina/química Berberina/farmacologia Linhagem Celular Tumoral Desoxicitidina/farmacologia Regulação para Baixo/efeitos dos fármacos Seres Humanos NF-kappa B/imunologia Pâncreas/efeitos dos fármacos Pâncreas/imunologia Neoplasias Pancreáticas/imunologia Phellodendron/química Extratos Vegetais/química Fator de Transcrição STAT3/imunologia Transdução de Sinais/efeitos dos fármacos [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Inflammatory Agents); 0 (Antimetabolites, Antineoplastic); 0 (NF-kappa B); 0 (Nexrutine); 0 (Plant Extracts); 0 (STAT3 Transcription Factor); 0I8Y3P32UF (Berberine); 0W860991D6 (Deoxycytidine); B76N6SBZ8R (gemcitabine) [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170901 [Lr] Data última revisão: 170901 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160522 [St] Status: MEDLINE [do] DOI: 10.1002/mc.22503

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[PMID]: 27491752 [Au] Autor: Yan C; Zhang YD; Wang XH; Geng SD; Wang TY; Sun M; Liang W; Zhang WQ; Zhang XD; Luo H [Ad] Endereço: An Shun City People's Hospital, Anshun, People's Republic of China. [Ti] Título: Tirucallane-type triterpenoids from the fruits of Phellodendron chinense Schneid and their cytotoxic activities. [So] Source: Fitoterapia;113:132-8, 2016 Sep. [Is] ISSN: 1873-6971 [Cp] País de publicação: Netherlands [La] Idioma: eng [Ab] Resumo: Eleven triterpenoids were isolated from the fruits of Phellodendron chinense Schneid, and their structures were determined by spectroscopic analysis. The results show that four new tirucallane-type triterpenoids 1, 2, 5, and 6 and seven known compounds 3, 4, 7, 8, 9, 10, and 11 were isolated. Structurally, compound 6 was uncommon; it has a chlorine atom instead of a methyl group at the C-20 position. The cytotoxicities of the compounds was evaluated against the in vitro proliferation of four human tumor cell lines HEL, K562, MDA, and PC3 using adriamycin as the positive control. Compound 1 showed a similar cytotoxicity as the positive control; compounds 3 and 10 showed moderate cytotoxicities compared to the control (P<0.05). This indicates that these compounds have great potential for the development of new antitumor drugs. [Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/química Frutas/química Phellodendron/química Triterpenos/química [Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/isolamento & purificação Linhagem Celular Tumoral Seres Humanos Estrutura Molecular Extratos Vegetais/química Triterpenos/isolamento & purificação [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antineoplastic Agents, Phytogenic); 0 (Plant Extracts); 0 (Triterpenes); 0 (tirucallane) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170131 [Lr] Data última revisão: 170131 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160806 [St] Status: MEDLINE

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[PMID]: 27484768 [Au] Autor: Kim JH; Weeratunga P; Kim MS; Nikapitiya C; Lee BH; Uddin MB; Kim TH; Yoon JE; Park C; Ma JY; Kim H; Lee JS [Ad] Endereço: College of Veterinary Medicine, Chungnam National University, 220 Gung-Dong, Yuseong-Gu, Daejeon, 305-764, Republic of Korea. [Ti] Título: Inhibitory effects of an aqueous extract from Cortex Phellodendri on the growth and replication of broad-spectrum of viruses in vitro and in vivo. [So] Source: BMC Complement Altern Med;16:265, 2016 Aug 02. [Is] ISSN: 1472-6882 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Cortex Phellodendri (C. Phellodendri), the dried trunk bark of Phellodendron amurense Ruprecht, has been known as a traditional herbal medicine, showing several bioactivities. However, antiviral activity of C. Phellodendri aqueous extract (CP) not reported in detail, particularly aiming the prophylactic effectiveness. METHODS: In vitro CP antiviral activity evaluated against Influenza A virus (PR8), Vesicular Stomatitis Virus (VSV), Newcastle Disease Virus (NDV), Herpes Simplex Virus (HSV), Coxsackie Virus (H3-GFP) and Enterovirus-71 (EV-71) infection on immune (RAW264.7) and epithelial (HEK293T/HeLa) cells. Such antiviral effects were explained by the induction of antiviral state which was determined by phosphorylation of signal molecules, secretion of IFNs and cytokines, and cellular antiviral mRNA expression. Furthermore, Compounds present in the aqueous fractions confirmed by HPLC analysis and evaluated their anti-viral activities. Additionally, in vivo protective effect of CP against divergent influenza A subtypes was determined in a BALB/c mouse infection model. RESULTS: An effective dose of CP significantly reduced the virus replication both in immune and epithelial cells. Mechanically, CP induced mRNA expression of anti-viral genes and cytokine secretion in both RAW264.7 and HEK293T cells. Furthermore, the main compound identified was berberine, and shows promising antiviral properties similar to CP. Finally, BALB/c mice treated with CP displayed higher protection levels against lethal doses of highly pathogenic influenza A subtypes (H1N1, H5N2, H7N3 and H9N2). CONCLUSION: CP including berberine play an immunomodulatory role with broad spectrum antiviral activity, due to induction of antiviral state via type I IFN stimulation mechanism. Consequently, C. Phellodendri could be a potential source for promising natural antivirals or to design other antiviral agents for animal and humans. [Mh] Termos MeSH primário: Antivirais/farmacologia Phellodendron/química Casca de Planta/química Extratos Vegetais/farmacologia Replicação Viral/efeitos dos fármacos Vírus/efeitos dos fármacos [Mh] Termos MeSH secundário: Animais Antivirais/química Células HEK293 Células HeLa Seres Humanos Camundongos Camundongos Endogâmicos BALB C Extratos Vegetais/química Células RAW 264.7 [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Antiviral Agents); 0 (Plant Extracts) [Em] Mês de entrada: 1701 [Cu] Atualização por classe: 170220 [Lr] Data última revisão: 170220 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160804 [St] Status: MEDLINE [do] DOI: 10.1186/s12906-016-1206-x

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[PMID]: 27298183 [Au] Autor: Zhang Z; Zhang Y; Zhang Z; Yao H; Liu H; Zhang B; Liao Y [Ad] Endereço: Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine, Ministry of Education/Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College. [Ti] Título: Comparative Analysis of DNA Barcoding and HPLC Fingerprint to Trace Species of Phellodendri Cortex, an Important Traditional Chinese Medicine from Multiple Sources. [So] Source: Biol Pharm Bull;39(8):1325-30, 2016 Aug 01. [Is] ISSN: 1347-5215 [Cp] País de publicação: Japan [La] Idioma: eng [Ab] Resumo: Phellodendri Cortex is derived from the dried barks of Phellodendron genus species, has been extensively used in traditional Chinese medicine. The cortex is divided into two odorless crude drugs Guanhuangbo and Huangbo. Historically, it has been difficult to distinguish their identities due to a lack of identification methods. This study was executed to confirm the identity and to ensure the species traceability of Phellodendri Cortex. In the current study, analysis is based on the internal transcribed spacer (ITS) and psbA-trnH intergenic spacer (psbA-trnH) barcodes and HPLC fingerprint was carried out to guarantee the species traceability of Guanhuangbo and Huangbo. DNA barcoding data successfully identified the three plants of the Phellodendron genus species by ITS+psbA-trnH, with the ability to distinguish the species origin of Huangbo. Moreover, the psbA-trnH data distinguished Guanhuangbo and Huangbo except to trace species. The HPLC fingerprint data showed that Guanhuangbo was clearly different from Huangbo, but there was no difference between the two origins of Huangbo. Additionally, the result of hierarchical clustering analysis, based on chlorogenic acid, phellodendrine, magnoflorine, jatrorrhizine, palmatine and berberine, was consistent with the HPLC fingerprint analysis. These results show that DNA barcoding and HPLC fingerprint can discriminate Guanhuangbo and Huangbo. However, DNA barcoding is more powerful than HPLC fingerprint for species traceability in the identification of related species that are genetically similar. DNA barcoding is a useful scientific tool to accurately confirm the identities of medicinal materials from multiple sources. [Mh] Termos MeSH primário: Phellodendron [Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão Código de Barras de DNA Taxonômico DNA Intergênico/genética DNA de Plantas/genética Medicina Tradicional Chinesa Phellodendron/química Phellodendron/classificação Phellodendron/genética Casca de Planta/química Casca de Planta/classificação Casca de Planta/genética [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (DNA, Intergenic); 0 (DNA, Plant) [Em] Mês de entrada: 1702 [Cu] Atualização por classe: 170201 [Lr] Data última revisão: 170201 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160615 [St] Status: MEDLINE [do] DOI: 10.1248/bpb.b16-00210

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[PMID]: 27285935 [Au] Autor: Bressan MC; Almeida J; Santos Silva J; Bettencourt C; Francisco A; Gama LT [Ti] Título: Carcass characteristics and fat depots in Iberian and F Large White × Landrace pigs intensively finished or raised outdoors in oak-tree forests. [So] Source: J Anim Sci;94(6):2592-602, 2016 Jun. [Is] ISSN: 1525-3163 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: A factorial experiment was performed with 117 barrows belonging to the Iberian (IB) and crossbred F Large White × Landrace (F) genetic groups, either intensively finished (IN) or finished outdoors on pasture in an oak and cork tree forest (EX). Information was collected on carcass weight, yield, and dimensions; weight of organs, carcass cuts, and abdominal fat depots; backfat depth; measurements of the longissimus thoracis (LT); and yield of different leg tissues. For the 41 slaughter and carcass traits analyzed, the interaction between genetic group and finishing system was significant ( < 0.05) in 18 traits, and overall, there was a more pronounced influence of genetic group than of finishing system. In most variables, particularly those related with fat deposition, the interaction reflected mostly changes in mean differences among genetic groups rather than in their ranking, where IB pigs consistently produced fatter carcasses, regardless of the finishing system. Liver weight in IB-EX pigs was lower by nearly 8% when compared with F-EX or IB-IN pigs, but the opposite pattern was found in F pigs, where liver weight in F-EX pigs was higher by 16% relative to IB-EX pigs or to F-IN pigs. The deposition of adipose tissue was much larger ( < 0.05) in IB pigs compared with F pigs, with means for fat depots in IB pigs that were higher by about 25% in total abdominal fat, 94% in dorsal fat depth, 72% in intermuscular plus subcutaneous fat in the leg, and over 300% in intramuscular fat (IMF). The deposition of lean tissue was much lower in IB pigs ( < 0.05), with means for trimmed loin weight corresponding to about one-half of the means obtained in F pigs, whereas lean percentage in the leg of IB pigs was about two-thirds of the mean in F pigs and the mean area of the LT was nearly one-half of that observed in F pigs in the same finishing system ( < 0.05). A strong correlation was observed between the various fat depots when the full data set was considered (correlations of IMF with abdominal fat and backfat depth of 0.65 and 0.83, respectively; < 0.05), but the correlations were much smaller when they were estimated within breed, particularly for IB pigs (-0.10 and 0.20 for the correlations of IMF with abdominal fat and backfat depth, respectively; > 0.05), indicating that it is feasible to reduce subcutaneous and abdominal fat without compromising IMF and meat quality. [Mh] Termos MeSH primário: Gorduras/análise Qualidade dos Alimentos Florestas Quercus Carne Vermelha/análise Sus scrofa/crescimento & desenvolvimento [Mh] Termos MeSH secundário: Gordura Abdominal/anatomia & histologia Tecido Adiposo Animais Composição Corporal Peso Corporal Cruzamento Cadáver Feminino Masculino Músculos Paraespinais/anatomia & histologia Phellodendron Portugal Gordura Subcutânea/anatomia & histologia Sus scrofa/anatomia & histologia Sus scrofa/genética Árvores [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE [Nm] Nome de substância: 0 (Fats) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170817 [Lr] Data última revisão: 170817 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 160611 [St] Status: MEDLINE [do] DOI: 10.2527/jas.2016-0276

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[PMID]: 26669678 [Au] Autor: Li C; Xie J; Chen X; Mo Z; Wu W; Liang Y; Su Z; Li Q; Li Y; Su Z; Yang X [Ad] Endereço: School of Chinese Materia Medica, Guangzhou University of Chinese Medicine, Guangzhou, P. R. China. [Ti] Título: Comparison of Helicobacter pylori Urease Inhibition by Rhizoma Coptidis, Cortex Phellodendri and Berberine: Mechanisms of Interaction with the Sulfhydryl Group. [So] Source: Planta Med;82(4):305-11, 2016 Mar. [Is] ISSN: 1439-0221 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Rhizoma Coptidis, Cortex Phellodendri, and berberine were reported to inhibit Helicobacter pylori. However, the underlying mechanism remained elusive. Urease plays a vital role in H. pylori colonization and virulence. In this work, aqueous extracts of Rhizoma Coptidis, Cortex Phellodendri of different origins, and purified berberine were investigated against H. pylori urease and jack bean urease to elucidate the inhibitory capacity, kinetics, and mechanism. Results showed that berberine was the major chemical component in Rhizoma Coptidis and Cortex Phellodendri, and the content of berberine in Rhizoma Coptidis was higher than in Cortex Phellodendri. The IC50 values of Rhizoma Coptidis were significantly lower than those Cortex Phellodendri and purified berberine, of which Coptis chinensis was shown to be the most active concentration- and time-dependent urease inhibitor. The Lineweaver-Burk plot analysis indicated that the inhibition pattern of C. chinensis against urease was noncompetitive for both H. pylori urease and jack bean urease. Thiol protectors (L-cysteine, glutathione, and dithiothreithol) significantly protected urease from the loss of enzymatic activity, while fluoride and boric acid showed weaker protection, indicating the active-site sulfhydryl group was possibly responsible for its inhibition. Furthermore, the urease inhibition proved to be reversible since C. chinensis-blocked urease could be reactivated by glutathione. The results suggested that the anti-urease activity of Rhizoma Coptidis was superior to that of Cortex Phellodendri and berberine, which was believed to be more likely to correlate to the content of total alkaloids rather than berberine monomer. The concentration- and time-dependent, reversible, and noncompetitive inhibition against urease by C. chinensis might be attributed to its interaction with the sulfhydryl group of the active site of urease. [Mh] Termos MeSH primário: Coptis/química Medicamentos de Ervas Chinesas/farmacologia Helicobacter pylori/efeitos dos fármacos Phellodendron/química Urease/antagonistas & inibidores [Mh] Termos MeSH secundário: Berberina/farmacologia Canavalia/enzimologia China Medicamentos de Ervas Chinesas/química Helicobacter pylori/enzimologia [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Drugs, Chinese Herbal); 0 (huangbai); 0I8Y3P32UF (Berberine); EC 3.5.1.5 (Urease) [Em] Mês de entrada: 1610 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151217 [St] Status: MEDLINE [do] DOI: 10.1055/s-0035-1558229

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[PMID]: 26421730 [Au] Autor: Boost M; Yau P; Yap M; Cho P [Ad] Endereço: Squina Centre for Infection Control, School of Nursing, The Hong Kong Polytechnic University, Hong Kong. Electronic address: maureen.boost@polyu.edu.hk. [Ti] Título: Determination of cytotoxicity of traditional Chinese medicine herbs, Rhizoma coptidis, Radix scutellariae, and Cortex phellodendri, by three methods. [So] Source: Cont Lens Anterior Eye;39(2):128-32, 2016 Apr. [Is] ISSN: 1476-5411 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: Many herbs are used in traditional Chinese medicine TCM) for treatment of infections but their properties, in particular, their effects on normal cells have received little attention. This study investigated the cytotoxic properties of three TCM herbs with potential use in prevention and treatment of ocular infections, including Acanthamoeba keratitis. METHOD: The study investigated cytotoxic effects of the herbal extracts of Rhizoma coptidis, Radix scutellariae, and Cortex phellodendri on human corneal epithelial cells using trypan blue staining, MTT production, and flow cytometry. Differences between herbs were determined using repeated measures one-way analysis of variance, followed by paired t-tests where appropriate. RESULTS: These three herbs appeared to lack cytotoxicity when tested with trypan blue and MTT, but flow cytometry revealed that R. coptidis led to cell membrane damage. CONCLUSION: Lack of cytotoxicity of R. scutellariae and C. phellodendri extracts suggest that these are potentially suitable for use in ocular preparations. Only flow cytometry was able to accurately predict cytotoxic effects of extracts of TCM herbs on HCEC, demonstrating the importance of using a sensitive method of detection of cytotoxicity. [Mh] Termos MeSH primário: Coptis/química Medicamentos de Ervas Chinesas/toxicidade Epitélio Anterior/efeitos dos fármacos Phellodendron/química Extratos Vegetais/toxicidade Scutellaria baicalensis/química [Mh] Termos MeSH secundário: Anexina A5/metabolismo Linhagem Celular Sobrevivência Celular Corantes Epitélio Anterior/patologia Citometria de Fluxo Seres Humanos Medicina Tradicional Chinesa Coloração e Rotulagem Sais de Tetrazólio Tiazóis Testes de Toxicidade/métodos Azul Tripano [Pt] Tipo de publicação: COMPARATIVE STUDY; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (Annexin A5); 0 (Coloring Agents); 0 (Drugs, Chinese Herbal); 0 (Plant Extracts); 0 (Tetrazolium Salts); 0 (Thiazoles); EUY85H477I (thiazolyl blue); I2ZWO3LS3M (Trypan Blue) [Em] Mês de entrada: 1612 [Cu] Atualização por classe: 161230 [Lr] Data última revisão: 161230 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 151001 [St] Status: MEDLINE

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