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  1 / 18 MEDLINE  
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[PMID]:28151972
[Au] Autor:Lima DF; de Lima LI; Rocha JA; de Andrade IM; Grazina LG; Villa C; Meira L; Véras LM; Azevedo IF; Biase AG; Costa J; Oliveira MB; Mafra I; Leite JR
[Ad] Endereço:Núcleo de Pesquisa em Biodiversidade e Biotecnologia, Campus Ministro Reis Velloso, Universidade Federal do Piauí, Parnaíba, Piauí, Brazil.
[Ti] Título:Seasonal change in main alkaloids of jaborandi (Pilocarpus microphyllus Stapf ex Wardleworth), an economically important species from the Brazilian flora.
[So] Source:PLoS One;12(2):e0170281, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pilocarpus microphyllus Stapf ex Wardleworth (jaborandi, Rutaceae) is one of the most important Brazilian medicinal species owing to its content of pilocarpine (PIL), an alkaloid used for treating glaucoma and xerostomia. This species contains another alkaloid, epiisopiloturine (EPI), which has demonstrated effectiveness against schistosomiasis. The aim of this work was to assess seasonal changes of PIL and EPI in three populations of cultivated P. microphyllus from northeastern Brazil over one year, including the dry and rainy seasons. Alkaloid profiles were correlated to phenotypic and genetic patterns in the morphological and molecular characterizations. PIL was the primary alkaloid and its levels differed among populations in all months except September. The S01 population (green line) showed an especially high PIL content compared to populations S02 and S03 (traditional line), which had similar alkaloid contents. PIL content gradually decreased in the three populations in the rainy season.EPI content was significantly different between the green line (S01) and the traditional line (S02 and S03).S01 had a significantly lower EPI content in all months, demonstrating that it was not the best source for EPI extraction. Inter simple sequence repeat (ISSR) markers and morphological analyses clearly separated S01 from S02 and S03, in agreement with the alkaloid results. This study shows the first correlation between the chemical, morphological, and molecular markers of P. microphyllus and highlights the potential benefits of a multidisciplinary research approach aimed at supporting both industry and conservation of natural resources.
[Mh] Termos MeSH primário: Alcaloides/análise
Pilocarpus/química
Plantas Medicinais/química
[Mh] Termos MeSH secundário: 4-Butirolactona/análogos & derivados
4-Butirolactona/análise
Brasil
DNA de Plantas/genética
Genética Populacional
Imidazóis/análise
Repetições de Microssatélites
Pilocarpina/análise
Pilocarpus/anatomia & histologia
Pilocarpus/genética
Folhas de Planta/anatomia & histologia
Folhas de Planta/química
Folhas de Planta/genética
Plantas Medicinais/anatomia & histologia
Plantas Medicinais/genética
Estações do Ano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (DNA, Plant); 0 (Imidazoles); 0 (epiisopiloturine); 01MI4Q9DI3 (Pilocarpine); OL659KIY4X (4-Butyrolactone)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170814
[Lr] Data última revisão:
170814
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0170281


  2 / 18 MEDLINE  
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[PMID]:28111828
[Au] Autor:Rocha JA; Andrade IM; Véras LM; Quelemes PV; Lima DF; Soares MJ; Pinto PL; Mayo SJ; Ivanova G; Rangel M; Correia M; Mafud AC; Mascarenhas YP; Delerue-Matos C; de Moraes J; Eaton P; Leite JR
[Ad] Endereço:Programa de Mestrado em Biotecnologia (PPGBiotec), Campus de Parnaíba, Universidade Federal do Piauí (UFPI), Parnaíba, PI, Brazil.
[Ti] Título:Anthelmintic, Antibacterial and Cytotoxicity Activity of Imidazole Alkaloids from Pilocarpus microphyllus Leaves.
[So] Source:Phytother Res;31(4):624-630, 2017 Apr.
[Is] ISSN:1099-1573
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Pilocarpus microphyllus Stapf ex Wardlew (Rutaceae), popularly known as jaborandi, is a plant native to the northern and northeastern macroregions of Brazil. Several alkaloids from this species have been isolated. There are few reports of antibacterial and anthelmintic activities for these compounds. In this work, we report the antibacterial and anthelmintic activity of five alkaloids found in P. microphyllus leaves, namely, pilosine, epiisopilosine, isopilosine, epiisopiloturine and macaubine. Of these, only anthelmintic activity of one of the compounds has been previously reported. Nuclear magnetic resonance, HPLC and mass spectrometry were combined and used to identify and confirm the structure of the five compounds. As regards the anthelmintic activity, the alkaloids were studied using in vitro assays to evaluate survival time and damaged teguments for Schistosoma mansoni adult worms. We found epiisopilosine to have anthelmintic activity at very low concentrations (3.125 µg mL ); at this concentration, it prevented mating, oviposition, reducing motor activity and altered the tegument of these worms. In contrast, none of the alkaloids showed antibacterial activity. Additionally, alkaloids displayed no cytotoxic effect on vero cells. The potent anthelmintic activity of epiisopilosine indicates the potential of this natural compound as an antiparasitic agent. Copyright © 2017 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Alcaloides/química
Anti-Helmínticos/química
Antibacterianos/química
Imidazóis/química
Pilocarpus/química
Extratos Vegetais/química
Folhas de Planta/química
[Mh] Termos MeSH secundário: 4-Butirolactona/análogos & derivados
Animais
Imidazóis/farmacologia
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Anthelmintics); 0 (Anti-Bacterial Agents); 0 (Imidazoles); 0 (Plant Extracts); 0 (epiisopiloturine); 7GBN705NH1 (imidazole); OL659KIY4X (4-Butyrolactone)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170503
[Lr] Data última revisão:
170503
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170124
[St] Status:MEDLINE
[do] DOI:10.1002/ptr.5771


  3 / 18 MEDLINE  
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[PMID]:28056423
[Au] Autor:Nicolau LAD; Carvalho NS; Pacífico DM; Lucetti LT; Aragão KS; Véras LMC; Souza MHLP; Leite JRSA; Medeiros JVR
[Ad] Endereço:Department of Physiology and Pharmacology, Federal University of Ceará, Fortaleza, CE, Brazil.
[Ti] Título:Epiisopiloturine hydrochloride, an imidazole alkaloid isolated from Pilocarpus microphyllus leaves, protects against naproxen-induced gastrointestinal damage in rats.
[So] Source:Biomed Pharmacother;87:188-195, 2017 Mar.
[Is] ISSN:1950-6007
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:OBJECTIVE: This study aimed to investigate the protective effect of epiisopiloturine hydrochloride (EPI), an imidazole alkaloid, on NAP-induced gastrointestinal damage in rats. METHODS: Initially, rats were pretreated with 0.5% carboxymethylcellulose (vehicle) or EPI (3, 10 and 30mg/kg, p.o. or i.p., groups 3-5, respectively) twice daily, for 2days. After 1h, NAP (80mg/kg, p.o.) was given. The control group received only vehicle (group 1) or vehicle+naproxen (group 2). Rats were euthanized on 2nd day, 4h after NAP treatment. Stomachs lesions were measured. Samples were collected for histological evaluation and glutathione (GSH), malonyldialdehyde (MDA), myeloperoxidase (MPO), and cytokines levels. Moreover, gastric mucosal blood flow (GMBF) was evaluated. RESULTS: EPI pretreatment prevented NAP-induced macro and microscopic gastric damage with a maximal effect at 10mg/kg. Histological analysis revealed that EPI decreased scores of damage caused by NAP. EPI reduced MPO (3.4±0.3U/mg of gastric tissue) and inhibited changes in MDA (70.4±8.3mg/g of gastric tissue) and GSH (246.2±26.4mg/g of gastric tissue). NAP increased TNF-α levels, and this effect was reduced by EPI pretreatment. Furthermore, EPI increased GMBF by 15% compared with the control group. CONCLUSION: Our data show that EPI protects against NAP-induced gastric and intestinal damage by reducing pro-inflammatory cytokines, reducing oxidative stress, and increasing GMBF.
[Mh] Termos MeSH primário: 4-Butirolactona/análogos & derivados
Alcaloides/uso terapêutico
Gastroenteropatias/prevenção & controle
Imidazóis/uso terapêutico
Naproxeno/toxicidade
Pilocarpus
Extratos Vegetais/farmacologia
[Mh] Termos MeSH secundário: 4-Butirolactona/isolamento & purificação
4-Butirolactona/farmacologia
4-Butirolactona/uso terapêutico
Alcaloides/isolamento & purificação
Alcaloides/farmacologia
Animais
Relação Dose-Resposta a Droga
Mucosa Gástrica/efeitos dos fármacos
Mucosa Gástrica/patologia
Gastroenteropatias/induzido quimicamente
Gastroenteropatias/patologia
Imidazóis/isolamento & purificação
Imidazóis/farmacologia
Mucosa Intestinal/efeitos dos fármacos
Mucosa Intestinal/patologia
Masculino
Extratos Vegetais/isolamento & purificação
Folhas de Planta
Substâncias Protetoras/isolamento & purificação
Substâncias Protetoras/farmacologia
Ratos
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Imidazoles); 0 (Plant Extracts); 0 (Protective Agents); 0 (epiisopiloturine); 57Y76R9ATQ (Naproxen); OL659KIY4X (4-Butyrolactone)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170710
[Lr] Data última revisão:
170710
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170106
[St] Status:MEDLINE


  4 / 18 MEDLINE  
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Mazzafera, Paulo
[PMID]:26058143
[Au] Autor:Sawaya AC; Costa YD; Mazzafera P
[Ti] Título:Unraveling the biosynthesis of pilocarpine in Pilocarpus microphyllus.
[So] Source:Nat Prod Commun;10(5):721-4, 2015 May.
[Is] ISSN:1934-578X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pilocarpine is found exclusively in species of Pilocarpus and the presence of other imidazole alkaloids has been reported in several species of the genus. Pilocarpine has several important pharmaceutical applications. Although several imidazole alkaloids related to pilocarpine have been reported in the previous years, little is still known about its biosynthetic route. At most, histidine has been reported as the precursor of pilocarpine. Based on our own previous reports and in an experiment where pilocarpine and related alkaloids (pilosine, trachyllophiline and anhydropilosine) were supplied to P. microphyllus leaves and the alkaloid profile analyzed by UPLC-MS, we suggest a biosynthesis pathway for pilocarpine. Further experiments using labeled precursors associated with transcriptome data may allow us to understand the whole biosynthesis pathway and its genetic control.
[Mh] Termos MeSH primário: Pilocarpina/biossíntese
Pilocarpus/metabolismo
[Mh] Termos MeSH secundário: Vias Biossintéticas
Estrutura Molecular
Pilocarpina/química
Pilocarpus/química
Folhas de Planta/química
Folhas de Planta/metabolismo
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
01MI4Q9DI3 (Pilocarpine)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:150610
[Lr] Data última revisão:
150610
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150611
[St] Status:MEDLINE


  5 / 18 MEDLINE  
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Abreu, Ilka Nacif
Mazzafera, Paulo
Texto completo
[PMID]:25474486
[Au] Autor:Andreazza NL; Abreu IN; Sawaya AC; Mazzafera P
[Ad] Endereço:Departamento de Biologia Vegetal, Instituto de Biologia, CP 6109, Universidade Estadual de Campinas, 13083-970 Campinas, SP, Brazil.
[Ti] Título:Characterisation of the membrane transport of pilocarpine in cell suspension cultures of Pilocarpus microphyllus.
[So] Source:J Plant Physiol;175:37-47, 2015 Mar 01.
[Is] ISSN:1618-1328
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Pilocarpine is an alkaloid obtained from the leaves of Pilocarpus genus, with important pharmaceutical applications. Previous reports have investigated the production of pilocarpine by Pilocarpus microphyllus cell cultures and tried to establish the alkaloid biosynthetic route. However, the site of pilocarpine accumulation inside of the cell and its exchange to the medium culture is still unknown. Therefore, the aim of this study was to determine the intracellular accumulation of pilocarpine and characterise its transport across membranes in cell suspension cultures of P. microphyllus. Histochemical analysis and toxicity assays indicated that pilocarpine is most likely stored in the vacuoles probably to avoid cell toxicity. Assays with exogenous pilocarpine supplementation to the culture medium showed that the alkaloid is promptly uptaken but it is rapidly metabolised. Treatment with specific ABC protein transporter inhibitors and substances that disturb the activity of secondary active transporters suppressed pilocarpine uptake and release suggesting that both proteins may participate in the traffic of pilocarpine to inside and outside of the cells. As bafilomicin A1, a specific V-type ATPase inhibitor, had little effect and NH4Cl (induces membrane proton gradient dissipation) had moderate effect, while cyclosporin A and nifedipine (ABC proteins inhibitors) strongly inhibited the transport of pilocarpine, it is believed that ABC proteins play a major role in the alkaloid transport across membranes but it is not the exclusive one. Kinetic studies supported these results.
[Mh] Termos MeSH primário: Pilocarpina/metabolismo
Pilocarpus/metabolismo
ATPases Vacuolares Próton-Translocadoras/metabolismo
[Mh] Termos MeSH secundário: Transporte Biológico
Técnicas de Cultura de Células
Coffea/efeitos dos fármacos
Meios de Cultura
Ciclosporina/farmacologia
Cinética
Nifedipino/farmacologia
Pilocarpina/isolamento & purificação
Pilocarpina/toxicidade
Pilocarpus/química
Pilocarpus/genética
Piper/efeitos dos fármacos
Folhas de Planta/química
Folhas de Planta/genética
Folhas de Planta/metabolismo
Proteínas de Plantas/antagonistas & inibidores
Proteínas de Plantas/genética
Proteínas de Plantas/metabolismo
ATPases Vacuolares Próton-Translocadoras/antagonistas & inibidores
ATPases Vacuolares Próton-Translocadoras/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Culture Media); 0 (Plant Proteins); 01MI4Q9DI3 (Pilocarpine); 83HN0GTJ6D (Cyclosporine); EC 3.6.1.- (Vacuolar Proton-Translocating ATPases); I9ZF7L6G2L (Nifedipine)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150225
[Lr] Data última revisão:
150225
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141205
[St] Status:MEDLINE


  6 / 18 MEDLINE  
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[PMID]:23840522
[Au] Autor:Véras LM; Cunha VR; Lima FC; Guimarães MA; Vieira MM; Campelo YD; Sakai VY; Lima DF; Carvalho PS; Ellena JA; Silva PR; Vasconcelos LC; Godejohann M; Petrilli HM; Constantino VR; Mascarenhas YP; de Souza de Almeida Leite JR
[Ad] Endereço:Núcleo de Pesquisa em Biodiversidade e Biotecnologia, Universidade Federal do Piauí, Parnaíba, Piauí, Brazil ; Programa de Pós-Graduação em Biotecnologia - RENORBIO, Universidade Federal do Piauí, Teresina, Piauí, Brazil.
[Ti] Título:Industrial Scale Isolation, Structural and Spectroscopic Characterization of Epiisopiloturine from Pilocarpus microphyllus Stapf Leaves: A Promising Alkaloid against Schistosomiasis.
[So] Source:PLoS One;8(6):e66702, 2013.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This paper presents an industrial scale process for extraction, purification, and isolation of epiisopiloturine (EPI) (2(3H)-Furanone,dihydro-3-(hydroxyphenylmethyl)-4-[(1-methyl-1H-imidazol-4-yl)methyl]-, [3S-[3a(R*),4b]]), which is an alkaloid from jaborandi leaves (Pilocarpus microphyllus Stapf). Additionally for the first time a set of structural and spectroscopic techniques were used to characterize this alkaloid. EPI has shown schistomicidal activity against adults and young forms, as well as the reduction of the egg laying adult worms and low toxicity to mammalian cells (in vitro). At first, the extraction of EPI was done with toluene and methylene chloride to obtain a solution that was alkalinized with ammonium carbonate. The remaining solution was treated in sequence by acidification, filtration and alkalinization. These industrial procedures are necessary in order to remove impurities and subsequent application of the high performance liquid chromatography (HPLC). The HPLC was employed also to remove other alkaloids, to obtain EPI purity higher than 98%. The viability of the method was confirmed through HPLC and electrospray mass spectrometry, that yielded a pseudo molecular ion of m/z equal to 287.1 Da. EPI structure was characterized by single crystal X-ray diffraction (XRD), (1)H and (13)C nuclear magnetic resonance (NMR) in deuterated methanol/chloroform solution, vibrational spectroscopy and mass coupled thermal analyses. EPI molecule presents a parallel alignment of the benzene and the methyl imidazol ring separated by an interplanar spacing of 3.758 Å indicating a π-π bond interaction. The imidazole alkaloid melts at 225°C and decomposes above 230°C under air. EPI structure was used in theoretical Density Functional Theory calculations, considering the single crystal XRD data in order to simulate the NMR, infrared and Raman spectra of the molecule, and performs the signals attribution.
[Mh] Termos MeSH primário: 4-Butirolactona/análogos & derivados
Imidazóis/isolamento & purificação
Pilocarpus/química
Folhas de Planta/química
Esquistossomicidas/isolamento & purificação
[Mh] Termos MeSH secundário: 4-Butirolactona/química
4-Butirolactona/isolamento & purificação
Cristalografia por Raios X
Imidazóis/química
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Imidazoles); 0 (Plant Extracts); 0 (Schistosomicides); 0 (epiisopiloturine); OL659KIY4X (4-Butyrolactone)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130711
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0066702


  7 / 18 MEDLINE  
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[PMID]:23734744
[Au] Autor:Silva VG; Silva RO; Damasceno SR; Carvalho NS; Prudêncio RS; Aragão KS; Guimarães MA; Campos SA; Véras LM; Godejohann M; Leite JR; Barbosa AL; Medeiros JV
[Ad] Endereço:Biotechnology and Biodiversity Center Research (BIOTEC), Federal University of Piauí, Campus of Parnaíba, Avenida São Sebastião, 64202-020, Parnaíba-PI, Brazil.
[Ti] Título:Anti-inflammatory and antinociceptive activity of epiisopiloturine, an imidazole alkaloid isolated from Pilocarpus microphyllus.
[So] Source:J Nat Prod;76(6):1071-7, 2013 Jun 28.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The aim of this study was to investigate the antinociceptive and anti-inflammatory activities of epiisopiloturine (1), an imidazole alkaloid found in the leaves of Pilocarpus microphyllus. The anti-inflammatory activity of 1 was evaluated using several agents that induce paw edema and peritonitis in Swiss mice. Paw tissue and peritoneal fluid samples were obtained to determine myeloperoxidase (MPO) activity or tumor necrosis factor (TNF)-α and interleukin (IL)-1ß levels. The antinociceptive activity was evaluated by acetic acid-induced writhing, the hot plate test, and pain induction using formalin. Compared to vehicle treatment, pretreatment with 1 (0.1, 0.3, and 1 mg/kg, ip) of mice significantly reduced carrageenan-induced paw edema (p < 0.05). Furthermore, compound 1 at a dose of 1 mg/kg effectively inhibited edema induced by dextran sulfate, serotonin, and bradykinin, but had no effect on histamine-induced edema. The administration of 1 (1 mg/kg) following carrageenan-induced peritonitis reduced total and differential peritoneal leukocyte counts and also carrageenan-induced paw MPO activity and TNF-α and IL-1ß levels in the peritoneal cavity. Pretreatment with 1 also reduced acetic acid-induced writhing and inhibited the first and second phases of the formalin test, but did not alter response latency in the hot plate test. Pretreatment with naloxone reversed the antinociceptive effect of 1.
[Mh] Termos MeSH primário: 4-Butirolactona/análogos & derivados
Alcaloides/farmacologia
Analgésicos/farmacologia
Anti-Inflamatórios/farmacologia
Imidazóis/farmacologia
Pilocarpus/química
[Mh] Termos MeSH secundário: 4-Butirolactona/química
4-Butirolactona/farmacologia
Alcaloides/sangue
Alcaloides/química
Analgésicos/sangue
Analgésicos/química
Animais
Anti-Inflamatórios/sangue
Anti-Inflamatórios/química
Brasil
Imidazóis/química
Masculino
Camundongos
Estrutura Molecular
Neutrófilos/efeitos dos fármacos
Medição da Dor
Peroxidase/sangue
Peroxidase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Alkaloids); 0 (Analgesics); 0 (Anti-Inflammatory Agents); 0 (Imidazoles); 0 (epiisopiloturine); EC 1.11.1.7 (Peroxidase); OL659KIY4X (4-Butyrolactone)
[Em] Mês de entrada:1310
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130606
[St] Status:MEDLINE
[do] DOI:10.1021/np400099m


  8 / 18 MEDLINE  
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[PMID]:22683904
[Au] Autor:Manzali de Sá I; Elisabetsky E
[Ad] Endereço:Departamento de Botânica, Museu Nacional/Universidade Federal do Rio de Janeiro, Rio de Janeiro, Quinta da Boa Vista, s/n, São Cristóvão, 20940-040 Rio de Janeiro, RJ, Brazil. ivmanzali@gmail.com
[Ti] Título:Medical knowledge exchanges between Brazil and Portugal: an ethnopharmacological perspective.
[So] Source:J Ethnopharmacol;142(3):762-8, 2012 Aug 01.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: Like many traditional medical systems found at Latin America, the very existence of a Brazilian traditional medical system is debated. Despite the absence of written material and organized knowledge, there is little doubt that Brazilians from all regions and all social classes recognize and access an estimated 4000 plant species with alleged therapeutic purposes as well as medicinal practices ranging from bone setting to spiritual healing. This "Brazilian folk medicine" is usually described as a rich mixture of African, European, and Indigenous medical traditions. AIM OF THE STUDY: This study questions this view, and argues it is both simplistic and Eurocentric. MATERIALS AND METHODS: By scrutinizing the origins of the medical uses of Zingiberis officinale, Curcuma longa, Ruta officinalis, Cephaelis ipecacuanha, Pilocarpus pinnatifolius, and curare (Chondrodendron, Abuta and Curarea), we illustrate the intense circulation of materials during imperial times. We further discuss how these practices articulated with local medical knowledge, and exemplify some of the ways by which knowledge was produced, transformed, incorporated, and resignified over time. DISCUSSION: Though not a systematic or comprehensive analysis of Brazilian folk medicine development, these selected examples show that, in opposition to usual simplistic descriptions, complex and convoluted manners of medicinal plant development occurred over time to compound both the Brazilian and European pharmaceutical armamentarium.
[Mh] Termos MeSH primário: Disseminação de Informação
Medicina Tradicional
Plantas Medicinais
[Mh] Termos MeSH secundário: Brasil
Cephaelis
Curare
Curcuma
Etnofarmacologia
Gengibre
Seres Humanos
Pilocarpus
Portugal
Ruta
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
8063-06-7 (Curare)
[Em] Mês de entrada:1212
[Cu] Atualização por classe:120716
[Lr] Data última revisão:
120716
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120612
[St] Status:MEDLINE
[do] DOI:10.1016/j.jep.2012.05.058


  9 / 18 MEDLINE  
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[PMID]:22420337
[Au] Autor:Veras LM; Guimaraes MA; Campelo YD; Vieira MM; Nascimento C; Lima DF; Vasconcelos L; Nakano E; Kuckelhaus SS; Batista MC; Leite JR; Moraes J
[Ad] Endereço:Núcleo de Pesquisa em Biodiversidade e Biotecnologia, BIOTEC, Campus de Parnaíba, Universidade Federal do Piauí, Brazil.
[Ti] Título:Activity of epiisopiloturine against Schistosoma mansoni.
[So] Source:Curr Med Chem;19(13):2051-8, 2012.
[Is] ISSN:1875-533X
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Schistosomiasis, caused by blood flukes of the genus Schistosoma, still imposes a considerable public health burden on large parts of the world. The control of this disease depends almost exclusively on the drug praziquantel, and there are no alternative drugs in sight. Natural compounds have recently attracted significant attention due to their relevance to parasitic infection and potential development into new therapeutic agents. Epiisopiloturine is an imidazole alkaloid isolated from the leaves of Pilocarpus microphyllus (Rutaceae), a native plant from Brazil. Here, we report the in vitro effect of this drug on the survival time of Schistosoma mansoni of different ages, such as 3 h old and 1, 3, 5, and 7 days old schistosomula, 49-day-old adults, and on egg output by adult worms. Epiisopiloturine at a concentration of 300 µg/mL caused the death of all schistosomula within 120 h. Extensive tegumental alterations and death were observed when adult schistosomes had been exposed to 150 µg/mL of the epiisopiloturine. At the highest sub-lethal dose of alkaloid (100 µg/mL), a 100% reduction in egg laying of paired adult worms was observed. Additionally, epiisopiloturine showed selective antischistosomal activity and exhibited no cytotoxicity to mammalian cells. This report provides the first evidence that epiisopiloturine is able to kill S. mansoni of different ages and inhibit worm egg laying.
[Mh] Termos MeSH primário: 4-Butirolactona/análogos & derivados
Imidazóis/farmacologia
Schistosoma mansoni/efeitos dos fármacos
[Mh] Termos MeSH secundário: 4-Butirolactona/química
4-Butirolactona/isolamento & purificação
4-Butirolactona/farmacologia
4-Butirolactona/toxicidade
Animais
Cercopithecus aethiops
Imidazóis/química
Imidazóis/isolamento & purificação
Imidazóis/toxicidade
Camundongos
Pilocarpus/química
Reprodução/efeitos dos fármacos
Schistosoma mansoni/fisiologia
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Imidazoles); 0 (epiisopiloturine); 7GBN705NH1 (imidazole); OL659KIY4X (4-Butyrolactone)
[Em] Mês de entrada:1210
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120317
[St] Status:MEDLINE


  10 / 18 MEDLINE  
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Mazzafera, Paulo
Texto completo
[PMID]:20845264
[Au] Autor:Abreu IN; Choi YH; Sawaya AC; Eberlin MN; Mazzafera P; Verpoorte R
[Ad] Endereço:Plant Product and Food Quality Department, Scottish Crop Research Institute, Invergowrie Dundee, United Kingdom. ilka.abreu@scri.ac.uk
[Ti] Título:Metabolic alterations in different developmental stages of Pilocarpus microphyllus.
[So] Source:Planta Med;77(3):293-300, 2011 Feb.
[Is] ISSN:1439-0221
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Pilocarpine is an imidazole alkaloid that has been used for more than a century in glaucoma treatment. It is present in several species of the Pilocarpus genus (jaborandi), with its highest concentrations in P. microphyllus. In addition to pilocarpine, pilosine--an imidazole alkaloid without pharmacological use--is produced in high concentrations in mature plants. A metabolomic study was carried out on juvenile and mature plants to obtain information about pilocarpine metabolism at different developmental stages. Methanol-water and alkaloid extracts were analyzed by ¹H NMR and ESI-MS. Metabolic profiles from both techniques showed clear differences between various developmental stages. Intense signals in the aromatic region of the ¹H NMR spectrum and ions from pilosine and related alkaloids by ESI/MS were found only in extracts from mature plant. Two new imidazole alkaloids were identified by MS(n). Our results suggest that pilosine is produced exclusively in mature developmental stage, and juvenile plant material seems to be appropriate for further studies on pilocarpine biosynthesis.
[Mh] Termos MeSH primário: Imidazóis/metabolismo
Pilocarpina/biossíntese
Pilocarpus/metabolismo
[Mh] Termos MeSH secundário: Metaboloma
Espectrometria de Massas por Ionização por Electrospray
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Imidazoles); 0 (pilosine); 01MI4Q9DI3 (Pilocarpine)
[Em] Mês de entrada:1109
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100917
[St] Status:MEDLINE
[do] DOI:10.1055/s-0030-1250314



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