Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.875.755 [Categoria DeCS]
Referências encontradas : 127 [refinar]
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[PMID]:28774334
[Au] Autor:Jeon WY; Kim OS; Seo CS; Jin SE; Kim JA; Shin HK; Kim YU; Lee MY
[Ad] Endereço:K-herb Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon, 34054, Republic of Korea.
[Ti] Título:Inhibitory effects of Ponciri Fructus on testosterone-induced benign prostatic hyperplasia in rats.
[So] Source:BMC Complement Altern Med;17(1):384, 2017 Aug 03.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Benign prostatic hyperplasia (BPH) is non-cancerous condition of enlargement of the prostate, a common occurrence in older men. The immature fruits of Poncirus trifoliata (L.) Rafinesque (Rutaceae), Ponciri Fructus are widely used in traditional oriental medicine for the therapy of various diseases. However, little is known about the mechanism underlying the pathogenesis of BPH. In the present study, we investigated the protective effects of a Ponciri Fructus extract (PFE) on the development of BPH in a in a rat model of BPH induced by testosterone propionate (TP). METHODS: Male Sprague Dawley rats were used as a model of BPH after its induction by daily subcutaneous injections of TP/corn oil, for a period of four weeks. PFE was administrated daily 1 h before TP/corn oil injection by oral gavage at a dose level of 200 mg/kg during the 4 weeks of TP/corn oil injections. All rats were sacrificed at the end of the experiment, we measured the relative prostate weight, the levels of testosterone and dihydrotestosterone (DHT), histological changes, activities of antioxidant enzymes (catalase, glutathione peroxidase, glutathione reductase, and superoxide dismutase), and expression of proliferating cell nuclear antigen (PCNA). In addition, we also measured the inhibition (%) of 5α-reductase in the prostatic tissue. RESULTS: Our findings indicate that PFE significantly inhibited the development of BPH; decreased the relative prostate weight, the level of testosterone and DHT in serum and prostatic tissue, prostatic hyperplasia, expression of PCNA, and increased the antioxidant enzymes. Moreover, PFE showed a weak inhibitory activity on 5α-reductase. CONCLUSIONS: These results suggest that PFE may be used as a therapeutic agent for BPH via antiproliferative and antioxidant effects.
[Mh] Termos MeSH primário: Antioxidantes/uso terapêutico
Fitoterapia
Extratos Vegetais/uso terapêutico
Poncirus
Próstata/efeitos dos fármacos
Hiperplasia Prostática/tratamento farmacológico
[Mh] Termos MeSH secundário: Animais
Antioxidantes/metabolismo
Antioxidantes/farmacologia
Catalase/metabolismo
Colestenona 5 alfa-Redutase/metabolismo
Di-Hidrotestosterona/sangue
Modelos Animais de Doenças
Frutas
Glutationa Peroxidase/metabolismo
Glutationa Redutase/metabolismo
Masculino
Tamanho do Órgão
Extratos Vegetais/farmacologia
Antígeno Nuclear de Célula em Proliferação/metabolismo
Próstata/metabolismo
Próstata/patologia
Hiperplasia Prostática/induzido quimicamente
Hiperplasia Prostática/metabolismo
Ratos Sprague-Dawley
Superóxido Dismutase/metabolismo
Testosterona/sangue
Propionato de Testosterona
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts); 0 (Proliferating Cell Nuclear Antigen); 08J2K08A3Y (Dihydrotestosterone); 3XMK78S47O (Testosterone); EC 1.11.1.6 (Catalase); EC 1.11.1.9 (Glutathione Peroxidase); EC 1.15.1.1 (Superoxide Dismutase); EC 1.3.1.22 (Cholestenone 5 alpha-Reductase); EC 1.8.1.7 (Glutathione Reductase); WI93Z9138A (Testosterone Propionate)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170805
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1877-y


  2 / 127 MEDLINE  
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[PMID]:28160166
[Au] Autor:Arbona V; Zandalinas SI; Manzi M; González-Guzmán M; Rodriguez PL; Gómez-Cadenas A
[Ad] Endereço:Ecofisiologia i Biotecnologia Department Ciències Agràries i del Medi Natural, Universitat Jaume I, 12071, Castelló de la Plana, Spain. arbona@uji.es.
[Ti] Título:Depletion of abscisic acid levels in roots of flooded Carrizo citrange (Poncirus trifoliata L. Raf. × Citrus sinensis L. Osb.) plants is a stress-specific response associated to the differential expression of PYR/PYL/RCAR receptors.
[So] Source:Plant Mol Biol;93(6):623-640, 2017 Apr.
[Is] ISSN:1573-5028
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Soil flooding reduces root abscisic acid (ABA) levels in citrus, conversely to what happens under drought. Despite this reduction, microarray analyses suggested the existence of a residual ABA signaling in roots of flooded Carrizo citrange seedlings. The comparison of ABA metabolism and signaling in roots of flooded and water stressed plants of Carrizo citrange revealed that the hormone depletion was linked to the upregulation of CsAOG, involved in ABA glycosyl ester (ABAGE) synthesis, and to a moderate induction of catabolism (CsCYP707A, an ABA 8'-hydroxylase) and buildup of dehydrophaseic acid (DPA). Drought strongly induced both ABA biosynthesis and catabolism (CsNCED1, 9-cis-neoxanthin epoxycarotenoid dioxygenase 1, and CsCYP707A) rendering a significant hormone accumulation. In roots of flooded plants, restoration of control ABA levels after stress release was associated to the upregulation of CsBGLU18 (an ABA ß-glycosidase) that cleaves ABAGE. Transcriptional profile of ABA receptor genes revealed a different induction in response to soil flooding (CsPYL5) or drought (CsPYL8). These two receptor genes along with CsPYL1 were cloned and expressed in a heterologous system. Recombinant CsPYL5 inhibited ΔNHAB1 activity in vitro at lower ABA concentrations than CsPYL8 or CsPYL1, suggesting its better performance under soil flooding conditions. Both stress conditions induced ABA-responsive genes CsABI5 and CsDREB2A similarly, suggesting the occurrence of ABA signaling in roots of flooded citrus seedlings. The impact of reduced ABA levels in flooded roots on CsPYL5 expression along with its higher hormone affinity reinforce the role of this ABA receptor under soil-flooding conditions and explain the expression of certain ABA-responsive genes.
[Mh] Termos MeSH primário: Ácido Abscísico/metabolismo
Citrus sinensis/metabolismo
Proteínas de Plantas/genética
Raízes de Plantas/metabolismo
Poncirus/metabolismo
[Mh] Termos MeSH secundário: Ácido Abscísico/genética
Citrus sinensis/genética
Sistema Enzimático do Citocromo P-450/genética
Sistema Enzimático do Citocromo P-450/metabolismo
Dioxigenases/genética
Dioxigenases/metabolismo
Secas
Inundações
Regulação da Expressão Gênica de Plantas
Genoma de Planta
Reguladores de Crescimento de Planta/metabolismo
Proteínas de Plantas/metabolismo
Poncirus/genética
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Growth Regulators); 0 (Plant Proteins); 0 (Recombinant Proteins); 72S9A8J5GW (Abscisic Acid); 9035-51-2 (Cytochrome P-450 Enzyme System); EC 1.13.11.- (Dioxygenases); EC 1.13.11.51 (9-cis-epoxy-carotenoid dioxygenase); EC 1.14.13.93 (abscisic acid 8'-hydroxylase)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:171031
[Lr] Data última revisão:
171031
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170205
[St] Status:MEDLINE
[do] DOI:10.1007/s11103-017-0587-7


  3 / 127 MEDLINE  
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[PMID]:27663409
[Au] Autor:Wu H; Fu B; Sun P; Xiao C; Liu JH
[Ad] Endereço:Key Laboratory of Horticultural Plant Biology, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China.
[Ti] Título:A NAC Transcription Factor Represses Putrescine Biosynthesis and Affects Drought Tolerance.
[So] Source:Plant Physiol;172(3):1532-1547, 2016 Nov.
[Is] ISSN:1532-2548
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Arginine decarboxylase (ADC)-mediated putrescine biosynthesis plays an important role in plant stress responses, but the transcriptional regulation of ADC in response to abiotic stress is not well understood. We isolated a NAM, ATAF1/2, and CUC (NAC) domain-containing transcription factor, PtrNAC72, from trifoliate orange (Poncirus trifoliata) by yeast one-hybrid screening. PtrNAC72, localized to the nucleus, binds specifically to the promoter of PtADC and acts as a transcriptional repressor. PtrNAC72 expression was induced by cold, drought, and abscisic acid. ADC messenger RNA abundance and putrescine levels were decreased in transgenic tobacco (Nicotiana nudicaulis) plants overexpressing PtrNAC72 but increased, compared with the wild type, in an Arabidopsis (Arabidopsis thaliana) transfer DNA insertion mutant, nac72 While transgenic tobacco lines overexpressing PtrNAC72 were more sensitive to drought, plants of the Arabidopsis nac72 mutant exhibited enhanced drought tolerance, consistent with the accumulation of reactive oxygen species in the tested genotypes. In addition, exogenous application of putrescine to the overexpression lines restored drought tolerance, while treatment with d-arginine, an ADC inhibitor, compromised the drought tolerance of nac72 Taken together, these results demonstrate that PtrNAC72 is a repressor of putrescine biosynthesis and may negatively regulate the drought stress response, at least in part, via the modulation of putrescine-associated reactive oxygen species homeostasis.
[Mh] Termos MeSH primário: Adaptação Fisiológica
Secas
Proteínas de Plantas/metabolismo
Poncirus/fisiologia
Putrescina/biossíntese
Fatores de Transcrição/metabolismo
[Mh] Termos MeSH secundário: Ácido Abscísico/farmacologia
Adaptação Fisiológica/efeitos dos fármacos
Adaptação Fisiológica/genética
Sequência de Aminoácidos
Antioxidantes/metabolismo
Arabidopsis/efeitos dos fármacos
Arabidopsis/genética
Sequência de Bases
Carboxiliases/metabolismo
Dessecação
Regulação da Expressão Gênica de Plantas/efeitos dos fármacos
Mutação/genética
Proteínas de Plantas/química
Proteínas de Plantas/genética
Plantas Geneticamente Modificadas
Poncirus/efeitos dos fármacos
Poncirus/genética
Regiões Promotoras Genéticas/genética
Ligação Proteica/efeitos dos fármacos
RNA Mensageiro/genética
RNA Mensageiro/metabolismo
Espécies Reativas de Oxigênio/metabolismo
Proteínas Repressoras/metabolismo
Alinhamento de Sequência
Análise de Sequência de Proteína
Estresse Fisiológico/genética
Frações Subcelulares/efeitos dos fármacos
Frações Subcelulares/metabolismo
Tabaco/genética
Fatores de Transcrição/química
Fatores de Transcrição/genética
Técnicas do Sistema de Duplo-Híbrido
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Proteins); 0 (RNA, Messenger); 0 (Reactive Oxygen Species); 0 (Repressor Proteins); 0 (Transcription Factors); 72S9A8J5GW (Abscisic Acid); EC 4.1.1.- (Carboxy-Lyases); EC 4.1.1.19 (arginine decarboxylase); V10TVZ52E4 (Putrescine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171101
[Lr] Data última revisão:
171101
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160925
[St] Status:MEDLINE


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[PMID]:27428366
[Au] Autor:Dong X; Liu G; Wu X; Lu X; Yan L; Muhammad R; Shah A; Wu L; Jiang C
[Ad] Endereço:Microelement Research Center, College of Resources and Environment, Huazhong Agricultural University, Wuhan, Hubei, 430070, PR China.
[Ti] Título:Different metabolite profile and metabolic pathway with leaves and roots in response to boron deficiency at the initial stage of citrus rootstock growth.
[So] Source:Plant Physiol Biochem;108:121-131, 2016 Nov.
[Is] ISSN:1873-2690
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:Boron (B) is a microelement required for higher plants, and B deficiency has serious negative effect on metabolic processes. We concentrated on the changes in metabolite profiles of trifoliate orange leaves and roots as a consequence of B deficiency at the initial stage of growth by gas chromatography-mass spectrometry (GC-MS)-based metabolomics. Enlargement and browning of root tips were observed in B-deficient plants, while any obvious symptom was not recorded in the leaves after 30 days of B deprivation. The distinct patterns of alterations in metabolites observed in leaves and roots due to B deficiency suggest the presence of specific organ responses to B starvation. The accumulation of soluble sugars was occurred in leaves, which may be attributed to down-regulated pentose phosphate pathway (PPP) and amino acid biosynthesis under B deficiency, while the amount of most amino acids in roots was increased, indicating that the effects of B deficiency on amino acids metabolism in trifoliate orange may be a consequence of disruptions in root tissues and decreased protein biosynthesis. Several important products of shikimate pathway were also significantly affected by B deficiency, which may be related to abnormal growth of roots induced by B deficiency. Conclusively, our results revealed a global perspective of the discriminative metabolism responses appearing between B-deprived leaves and roots and provided new insight into the relationship between B deficiency symptom in roots and the altered amino acids profiling and shikimate pathway induced by B deficiency during seedling establishment.
[Mh] Termos MeSH primário: Boro/deficiência
Folhas de Planta/metabolismo
Raízes de Plantas/metabolismo
Poncirus/crescimento & desenvolvimento
Poncirus/metabolismo
[Mh] Termos MeSH secundário: Aminoácidos/metabolismo
Boro/metabolismo
Cromatografia Gasosa-Espectrometria de Massas
Redes e Vias Metabólicas
Metabolômica/métodos
Via de Pentose Fosfato
Plântulas/crescimento & desenvolvimento
Plântulas/metabolismo
Ácido Chiquímico/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amino Acids); 29MS2WI2NU (Shikimic Acid); N9E3X5056Q (Boron)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170813
[Lr] Data última revisão:
170813
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160719
[St] Status:MEDLINE


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[PMID]:27402968
[Au] Autor:Zhang X; Wang W; Wang M; Zhang HY; Liu JH
[Ad] Endereço:Key Laboratory of Horticultural Plant Biology, College of Horticulture and Forestry Sciences, Huazhong Agricultural University, Wuhan 430070, China These authors contributed equally to this work.
[Ti] Título:The miR396b of Poncirus trifoliata Functions in Cold Tolerance by Regulating ACC Oxidase Gene Expression and Modulating Ethylene-Polyamine Homeostasis.
[So] Source:Plant Cell Physiol;57(9):1865-78, 2016 Sep.
[Is] ISSN:1471-9053
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:MicroRNAs (miRNAs) are non-coding regulatory molecules that play important roles in a variety of biological processes. Although a number of cold-responsive miRNAs have been computationally identified, functions and mechanisms of most miRNAs are not well understood. Herein, the function of trifoliate orange [Poncirus trifoliata (L.) Raf.] miRNA396b (ptr-miR396b) in cold tolerance and its potential regulatory module were investigated. Compared with the wild type (WT), transgenic lemon (Citrus limon) plants overexpressing ptr-MIR396b, the precursor of ptr-miR396b, displayed enhanced cold tolerance. Ptr-miR396b was experimentally confirmed to guide the cleavage of 1-aminocyclopropane-1-carboxylic acid oxidase (ACO). The overexpressing lines exhibited a reduction in ACO transcript levels and ethylene content compared with the WT, and the expression pattern of ACO was opposite to that of ptr-miR396b in response to cold stress. In addition, the transgenic lines exhibited higher levels of free polyamines and mRNA abundance of polyamine biosynthetic genes than WT plants under cold treatment, consistent with reduced reactive oxygen species (ROS) accumulation in the former. Taken together, this study demonstrates that ptr-miR396b positively regulates cold tolerance through reducing ACO transcript levels, thereby repressing ethylene synthesis and simultaneously promoting polyamine synthesis, leading to enhanced ROS scavenging. Identification of the ptr-miR396b-ACO regulatory module provides new insights into the molecular mechanism underlying the reduction of ethylene production under cold.
[Mh] Termos MeSH primário: Aminoácido Oxirredutases/genética
Resposta ao Choque Frio/fisiologia
Etilenos/metabolismo
MicroRNAs/genética
Poncirus/fisiologia
[Mh] Termos MeSH secundário: Aminoácido Oxirredutases/metabolismo
Citrus/genética
Regulação da Expressão Gênica de Plantas
Homeostase
Plantas Geneticamente Modificadas
Poliaminas/metabolismo
Poncirus/genética
RNA de Plantas
Espécies Reativas de Oxigênio/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Ethylenes); 0 (MicroRNAs); 0 (Polyamines); 0 (RNA, Plant); 0 (Reactive Oxygen Species); EC 1.4.- (Amino Acid Oxidoreductases); EC 1.4.3.- (1-aminocyclopropane-1-carboxylic acid oxidase)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170313
[Lr] Data última revisão:
170313
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160713
[St] Status:MEDLINE
[do] DOI:10.1093/pcp/pcw108


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[PMID]:27277128
[Au] Autor:Wu H; Acanda Y; Jia H; Wang N; Zale J
[Ad] Endereço:Plant Pathology Department, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, University of Florida, Lake Alfred, FL, 33850, USA. whao@ufl.edu.
[Ti] Título:Biolistic transformation of Carrizo citrange (Citrus sinensis Osb. × Poncirus trifoliata L. Raf.).
[So] Source:Plant Cell Rep;35(9):1955-62, 2016 Sep.
[Is] ISSN:1432-203X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:KEY MESSAGE: The development of transgenic citrus plants by the biolistic method. A protocol for the biolistic transformation of epicotyl explants and transgenic shoot regeneration of immature citrange rootstock, cv. Carrizo (Citrus sinensis Osb. × Poncirus trifoliata L. Raf.) and plant regeneration is described. Immature epicotyl explants were bombarded with a vector containing the nptII selectable marker and the gfp reporter. The number of independent, stably transformed tissues/total number of explants, recorded by monitoring GFP fluorescence 4 weeks after bombardment was substantial at 18.4 %, and some fluorescing tissues regenerated into shoots. Fluorescing GFP, putative transgenic shoots were micro-grafted onto immature Carrizo rootstocks in vitro, confirmed by PCR amplification of nptII and gfp coding regions, followed by secondary grafting onto older rootstocks grown in soil. Southern blot analysis indicated that all the fluorescing shoots were transgenic. Multiple and single copies of nptII integrations were confirmed in five regenerated transgenic lines. There is potential to develop a higher throughput biolistics transformation system by optimizing the tissue culture medium to improve shoot regeneration and narrowing the window for plant sampling. This system will be appropriate for transformation with minimal cassettes.
[Mh] Termos MeSH primário: Biolística/métodos
Citrus sinensis/genética
Cruzamentos Genéticos
Poncirus/genética
Transformação Genética
[Mh] Termos MeSH secundário: Southern Blotting
Fenótipo
Brotos de Planta/genética
Plantas Geneticamente Modificadas
Plântulas/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171004
[Lr] Data última revisão:
171004
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160610
[St] Status:MEDLINE
[do] DOI:10.1007/s00299-016-2010-2


  7 / 127 MEDLINE  
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[PMID]:27223695
[Au] Autor:Rodríguez-Gamir J; Primo-Millo E; Forner-Giner MÁ
[Ad] Endereço:Department of Citriculture and Vegetal Production, Valencian Institute of Agrarias Research, Moncada, Valencia, Spain.
[Ti] Título:An Integrated View of Whole-Tree Hydraulic Architecture. Does Stomatal or Hydraulic Conductance Determine Whole Tree Transpiration?
[So] Source:PLoS One;11(5):e0155246, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hydraulic conductance exerts a strong influence on many aspects of plant physiology, namely: transpiration, CO2 assimilation, growth, productivity or stress response. However we lack full understanding of the contribution of root or shoot water transport capacity to the total water balance, something which is difficult to study in trees. Here we tested the hypothesis that whole plant hydraulic conductance modulates plant transpiration using two different seedlings of citrus rootstocks, Poncirus trifoliata (L.) Raf. and Cleopatra mandarin (Citrus reshni Hort ex Tan.). The two genotypes presented important differences in their root or shoot hydraulic conductance contribution to whole plant hydraulic conductance but, even so, water balance proved highly dependent on whole plant conductance. Further, we propose there is a possible equilibrium between root and shoot hydraulic conductance, similar to that between shoot and root biomass production, which could be related with xylem anatomy.
[Mh] Termos MeSH primário: Citrus/fisiologia
Raízes de Plantas/fisiologia
Estômatos de Plantas/fisiologia
Transpiração Vegetal/fisiologia
Poncirus/fisiologia
Plântulas/fisiologia
Água/metabolismo
Xilema/fisiologia
[Mh] Termos MeSH secundário: Transporte Biológico Ativo/fisiologia
Citrus/anatomia & histologia
Raízes de Plantas/anatomia & histologia
Estômatos de Plantas/anatomia & histologia
Poncirus/anatomia & histologia
Plântulas/anatomia & histologia
Xilema/anatomia & histologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170706
[Lr] Data última revisão:
170706
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160526
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0155246


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[PMID]:27058520
[Au] Autor:Jia S; Gao Z; Yan S; Chen Y; Sun C; Li X; Chen K
[Ad] Endereço:Zhejiang Provincial Key Laboratory of Horticultural Plant Integrative Biology, Zhejiang University, Zijingang Campus, Hangzhou 310058, China. shengjia@zju.edu.cn.
[Ti] Título:Anti-Obesity and Hypoglycemic Effects of Poncirus trifoliata L. Extracts in High-Fat Diet C57BL/6 Mice.
[So] Source:Molecules;21(4):453, 2016 Apr 06.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:The present study investigated the possible anti-obesity and hypoglycemic effects of Poncirus trifoliata L. extracts. Mature fruit were divided into flavedo (PF) and juice sacs (PJ), and extracts from them were tested on C57BL/6 mice fed a high-fat diet (HFD) for thirteen weeks. Both fruit extracts (40 mg/kg body weight, respectively) showed anti-obesity and hypoglycemic effects. Consumption of PF and PJ extracts reduced body weight by 9.21% and 20.27%, respectively. Liver and adipose weights, fasting glucose, serum triglyceride (TG), and low density lipoprotein cholesterol (LDL-c) levels decreased significantly, while serum high density lipoprotein cholesterol (HDL-c) and oral glucose tolerance levels increased significantly in response to two fruit extracts. These effects were due in part to the modulation of serum insulin, leptin, and adiponectin. Furthermore, transcript levels of fatty acid synthase (FAS) and stearoyl-CoA desaturase 1 (SCD1) were reduced while those of carnitine palmitoyltransferase 1α (CPT1α) and insulin receptor substrate 2 (IRS2) were increased in the liver of C57BL/6 mice, which might be an important mechanism affecting lipid and glucose metabolism. Taken together, P. trifoliata fruit can be potentially used to prevent or treat obesity and associated metabolic disorders.
[Mh] Termos MeSH primário: Hipoglicemiantes/administração & dosagem
Obesidade/tratamento farmacológico
Extratos Vegetais/administração & dosagem
Poncirus/química
[Mh] Termos MeSH secundário: Animais
Glicemia/efeitos dos fármacos
Colesterol/sangue
Dieta Hiperlipídica/efeitos adversos
Seres Humanos
Hipoglicemiantes/química
Leptina/sangue
Fígado/efeitos dos fármacos
Fígado/metabolismo
Camundongos
Obesidade/sangue
Extratos Vegetais/química
Triglicerídeos/sangue
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Blood Glucose); 0 (Hypoglycemic Agents); 0 (Leptin); 0 (Plant Extracts); 0 (Triglycerides); 97C5T2UQ7J (Cholesterol)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160409
[St] Status:MEDLINE


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[PMID]:27025596
[Au] Autor:Dahro B; Wang F; Peng T; Liu JH
[Ad] Endereço:Key Laboratory of Horticultural Plant Biology (MOE), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan, 430070, China.
[Ti] Título:PtrA/NINV, an alkaline/neutral invertase gene of Poncirus trifoliata, confers enhanced tolerance to multiple abiotic stresses by modulating ROS levels and maintaining photosynthetic efficiency.
[So] Source:BMC Plant Biol;16:76, 2016 Mar 29.
[Is] ISSN:1471-2229
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Alkaline/neutral invertase (A/N-INV), an enzyme that hydrolyzes sucrose irreversibly into glucose and fructose, is essential for normal plant growth,development, and stress tolerance. However, the physiological and/or molecular mechanism underpinning the role of A/N-INV in abiotic stress tolerance is poorly understood. RESULTS: In this report, an A/N-INV gene (PtrA/NINV) was isolated from Poncirus trifoliata, a cold-hardy relative of citrus, and functionally characterized. PtrA/NINV expression levels were induced by cold, salt, dehydration, sucrose, and ABA, but decreased by glucose. PtrA/NINV was found to localize in both chloroplasts and mitochondria. Overexpression of PtrA/NINV conferred enhanced tolerance to multiple stresses, including cold, high salinity, and drought, as supported by lower levels of reactive oxygen species (ROS), reduced oxidative damages, decreased water loss rate, and increased photosynthesis efficiency, relative to wild-type (WT). The transgenic plants exhibited higher A/N-INV activity and greater reducing sugar content under normal and stress conditions. CONCLUSIONS: PtrA/NINV is an important gene implicated in sucrose decomposition, and plays a positive role in abiotic stress tolerance by promoting osmotic adjustment, ROS detoxification and photosynthesis efficiency. Thus, PtrA/NINV has great potential to be used in transgenic breeding for improvement of stress tolerance.
[Mh] Termos MeSH primário: Fotossíntese
Poncirus/enzimologia
Espécies Reativas de Oxigênio/metabolismo
beta-Frutofuranosidase/genética
[Mh] Termos MeSH secundário: Temperatura Baixa
Poncirus/metabolismo
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Reactive Oxygen Species); EC 3.2.1.26 (beta-Fructofuranosidase)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160331
[St] Status:MEDLINE
[do] DOI:10.1186/s12870-016-0761-0


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[PMID]:26985941
[Au] Autor:Liao L; Cao SY; Rong Y; Wang ZH
[Ad] Endereço:College of Horticulture, Sichuan Agricultural University, Chengdu, Sichuan, China.
[Ti] Título:Effects of grafting on key photosynthetic enzymes and gene expression in the citrus cultivar Huangguogan.
[So] Source:Genet Mol Res;15(1):15017690, 2016 Mar 04.
[Is] ISSN:1676-5680
[Cp] País de publicação:Brazil
[La] Idioma:eng
[Ab] Resumo:Grafting influences scion photosynthetic capacity and fruit quality. Ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco), which strongly affects photosynthetic rate, and Rubisco activase (RCA), which regulates Rubisco activity, are two key photosynthetic enzymes. However, little information is available regarding the effect of grafting on the concentration and expression of Rubisco and RCA in the citrus cultivar Huangguogan. The objective of this study was to investigate the effect of grafting Huangguogan plants onto trifoliate orange, tangerine, and orange on: 1) the concentration of Rubisco and RCA; 2) the mRNA levels of rbcL, rbcS, and rca; and 3) fruit quality. Overall, the results showed that when Huangguogan plants budded on tangerine and orange, they had better fruit quality, while on trifoliate orange they had higher Rubisco concentration. Tangerine and orange are probably the most suitable rootstocks for Huangguogan plants given the environmental conditions of Sichuan Province, China.
[Mh] Termos MeSH primário: Citrus/enzimologia
Produção Agrícola
Regulação da Expressão Gênica de Plantas
Proteínas de Plantas/genética
Ribulose-Bifosfato Carboxilase/genética
[Mh] Termos MeSH secundário: Citrus/genética
Citrus/fisiologia
Qualidade dos Alimentos
Frutas
Poncirus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Plant Proteins); 0 (rca protein, plant); EC 4.1.1.39 (Ribulose-Bisphosphate Carboxylase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170119
[Lr] Data última revisão:
170119
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160318
[St] Status:MEDLINE
[do] DOI:10.4238/gmr.15017690



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