Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.875.922 [Categoria DeCS]
Referências encontradas : 358 [refinar]
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[PMID]:28458421
[Au] Autor:Saikia B; Barua CC; Haloi P; Patowary P
[Ad] Endereço:Department of Pharmacology and Toxicology, College of Veterinary Science, Assam Agricultural University, Guwahati, Assam, India.
[Ti] Título:Anticholinergic, antihistaminic, and antiserotonergic activity of n-hexane extract of seeds on isolated tissue preparations: An study.
[So] Source:Indian J Pharmacol;49(1):42-48, 2017 Jan-Feb.
[Is] ISSN:1998-3751
[Cp] País de publicação:India
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: The aim of this study was to evaluate anticholinergic, antihistaminic, and antiserotonergic activity of the n-hexane extract of the seeds of (ZAHE) on isolated ileum of rat and guinea pig and fundus of rat. MATERIALS AND METHODS: ZAHE was prepared using soxhlet extraction and cumulative concentration response curves were constructed using various doses on the tissues for acetylcholine (ACh), 5-hydroxytryptamine (5-HT), and histamine with or without n-hexane extract. Atropine, ketanserin, and pheniramine maleate were used as antagonists for ACh, serotonin, and histamine, respectively. RESULTS: ZAHE-induced concentration-dependent inhibition of isolated ileum and fundus in rat and ileum of guinea pig. The half maximal effective concentration (EC ) of ACh in the presence of atropine (10 M; < 0.05) and ZAHE (1000 µg/ml; < 0.01) was significantly higher than EC of ACh alone. The EC of 5-HT in the presence of ketanserin (10 M; < 0.01) and ZAHE (1000 µg/ml; < 0.05) was higher than EC of 5-HT alone. Similarly, the EC of histamine in the presence of pheniramine maleate (10 M; < 0.01) and ZAHE (300 µg/ml; < 0.01 and 1000 µg/ml; < 0.05) was also significantly higher than EC of histamine alone. CONCLUSION: From the study, it was observed that ZAHE shows significant anticholinergic, antiserotonergic, and antihistaminic activity. The study provides sufficient evidence that the seeds can be used in gastric disorders, cough, chest infection, etc., as per folklore claims.
[Mh] Termos MeSH primário: Antagonistas Colinérgicos/farmacologia
Antagonistas dos Receptores Histamínicos/farmacologia
Extratos Vegetais/farmacologia
Antagonistas da Serotonina/farmacologia
Zanthoxylum/química
[Mh] Termos MeSH secundário: Acetilcolina/metabolismo
Animais
Antagonistas Colinérgicos/administração & dosagem
Antagonistas Colinérgicos/isolamento & purificação
Relação Dose-Resposta a Droga
Fundo Gástrico/efeitos dos fármacos
Fundo Gástrico/metabolismo
Cobaias
Hexanos/química
Histamina/metabolismo
Antagonistas dos Receptores Histamínicos/administração & dosagem
Antagonistas dos Receptores Histamínicos/isolamento & purificação
Íleo/efeitos dos fármacos
Íleo/metabolismo
Masculino
Extratos Vegetais/administração & dosagem
Ratos
Ratos Wistar
Sementes
Serotonina/metabolismo
Antagonistas da Serotonina/administração & dosagem
Antagonistas da Serotonina/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cholinergic Antagonists); 0 (Hexanes); 0 (Histamine Antagonists); 0 (Plant Extracts); 0 (Serotonin Antagonists); 2DDG612ED8 (n-hexane); 333DO1RDJY (Serotonin); 820484N8I3 (Histamine); N9YNS0M02X (Acetylcholine)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171229
[Lr] Data última revisão:
171229
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170502
[St] Status:MEDLINE
[do] DOI:10.4103/0253-7613.201025


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[PMID]:28873528
[Au] Autor:Zeng M; Wang J; Zhang M; Chen J; He Z; Qin F; Xu Z; Cao D; Chen J
[Ad] Endereço:State Key Laboratory of Food Science and Technology, Jiangnan University, 214122 Wuxi, China. Electronic address: mmzeng@jiangnan.edu.cn.
[Ti] Título:Inhibitory effects of Sichuan pepper (Zanthoxylum bungeanum) and sanshoamide extract on heterocyclic amine formation in grilled ground beef patties.
[So] Source:Food Chem;239:111-118, 2018 Jan 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study evaluated the inhibitory effects of Sichuan pepper (Zanthoxylum bungeanum) and sanshoamide extract on the formation of heterocyclic amines (HAs) in grilled beef patties. The following major HAs were detected and quantified: imidazopyridine (2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine, PhIP), imidazoquinoxalines (2-amino-3-methyl-3H-imidazo[4,5-f]quinoxaline, IQx; 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline, MeIOx; 2-amino-3,4,8-trimethyl-3H-imidazo[4,5-f]quinoxaline, 4,8-DiMeIQx) and ß-carbolines (9H-pyrido[3,4-b]indole, norharman; 1-methyl-9H-pyrido[3,4-b]indole, harman). The rates of PhIP, IQx, MeIQx and 4,8-DiMeIQx were inhibited significantly by 82%, 61%, 28% and 79%, respectively, in patties treated with a low-concentration of pepper (0.5%), and by 27%, 45%, 44% and 26%, respectively, following treatment with a low-concentration sanshoamide extract (0.005%). All inhibition rates exceeded 70% at pepper and sanshoamide extract concentrations of 1.0% and 0.010%, respectively. Harman and norharman could be significantly (p<0.05) enhanced by the addition of all concentrations of Sichuan pepper, however, sanshoamide extract had no significant (p>0.05) effect on them, although 0.005% of the extract could slightly increase the formation of them. Notably, significant correlations were observed between the HA inhibition rates and pepper or sanshoamide extract concentrations, especially for PhIP (r=0.87, p=2.30E-3), IQx (r=0.99, p=5.04E-7) and 4,8-DiMeIQx (r=0.88, p=1.96E-3). These results indicate that Sichuan pepper and sanshoamide extract could inhibit the formation of carcinogenic HAs during thermal preparation of protein-rich foods, and therefore might reduce the risk of chronic diseases associated with the long-term intake of HA-containing food products.
[Mh] Termos MeSH primário: Carne Vermelha
[Mh] Termos MeSH secundário: Aminas
Animais
Bovinos
Culinária
Compostos Heterocíclicos
Quinoxalinas
Zanthoxylum
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Amines); 0 (Heterocyclic Compounds); 0 (Quinoxalines)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171128
[Lr] Data última revisão:
171128
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170907
[St] Status:MEDLINE


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[PMID]:28899423
[Au] Autor:Tian Y; Zhang C; Guo M
[Ad] Endereço:Key Laboratory of Plant Germplasm Enhancement and Specialty Agriculture, Wuhan Botanical Garden, Chinese Academy of Sciences, Moshan, Wuchang, Wuhan, 430074, China.
[Ti] Título:Comparative study on alkaloids and their anti-proliferative activities from three Zanthoxylum species.
[So] Source:BMC Complement Altern Med;17(1):460, 2017 Sep 12.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Alkaloids have been considered as the most promising bioactive ingredients in plant species from the genus Zanthoxylum. This study reports on the compositions and contents of the Zanthoxylum alkaloids (ZAs) from three Zanthoxylum species, and their potential anti-proliferation activities. METHODS: An HPLC-UV/ESI-MS/MS method was established and employed to analyze the alkaloids in different Zanthoxylum extracts. The common and unique peaks and their relative contents were summarized and compared to evaluate the similarity and dissimilarity of the three Zanthoxylum species. Meanwhile, inhibitory activity tests to four carcinoma cell lines, i.e., stomach tumor cells (SGC-7901), cervical tumor cells (Hela), colon tumor cells (HT-29) and Hepatic tumor cells (Hep G2), were carried out in vitro to evaluate the bioactivities of the ZAs. RESULTS: Seventy peaks were detected in the crude total alkaloid samples, and 58 of them were identified. As a result, 13 common peaks were found in the extracts of all the three Zanthoxylum species, while some unique peaks were also observed in specific species, with 17 peaks in Z. simulans, 15 peaks in Z. ailanthoides and 11 peaks in Z. chalybeum, respectively. The comparison of the composition and relative contents indicated that alkaloids of benzophenanthridine type commonly present in all the three Zanthoxylum species with high relative contents among the others, which are 60.52% in Z. ailanthoides, 30.52% in Z. simulans and 13.84% in Z. chalybeum, respectively. In terms of activity test, Most of the crude alkaloids extracts showed remarkable inhibitory activities against various tumor cells, and the inhibitory rates ranged from 60.71 to 93.63% at a concentration of 200 µg/mL. However, SGC-7901 cells seemed to be more sensitive to the ZAs than the other three cancer cells. CONCLUSION: The alkaloid profiles detected in this work revealed significant differences in both structures and contents among Zanthoxylum species. The inhibitory rates for different cancer cells in this study indicated that the potential anti-cancer activity should be attributed to quaternary alkaloids in these three species, which will provide great guidance for further exploring this traditional medicinal resource as new healthcare products.
[Mh] Termos MeSH primário: Alcaloides/farmacologia
Antineoplásicos/farmacologia
Proliferação Celular/efeitos dos fármacos
Descoberta de Drogas
Extratos Vegetais/farmacologia
Zanthoxylum/química
[Mh] Termos MeSH secundário: Alcaloides/química
Alcaloides/isolamento & purificação
Antineoplásicos/química
Antineoplásicos/isolamento & purificação
Linhagem Celular Tumoral
Cromatografia Líquida de Alta Pressão
Seres Humanos
Estrutura Molecular
Extratos Vegetais/química
Zanthoxylum/classificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Antineoplastic Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171016
[Lr] Data última revisão:
171016
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170914
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1966-y


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[PMID]:28879837
[Au] Autor:Ma L; Xi JQ; Cao YH; Wang XY; Zheng SC; Yang CG; Yang LL; Mi QL; Li XM; Zhu ML; Mo MH
[Ad] Endereço:1​State Key Laboratory for Conservation and Utilization of Bio-Resources in Yunnan, Yunnan University, Kunming 650091, PR China.
[Ti] Título:Bacillus endozanthoxylicus sp. nov., an endophytic bacterium isolated from Zanthoxylum bungeanum Maxim leaves.
[So] Source:Int J Syst Evol Microbiol;67(10):3699-3705, 2017 Oct.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A Gram-stain-positive, rod-shaped, motile bacterium, designated as 1404 , was isolated from leaves of Chinese red pepper (Huajiao) (Zanthoxylum bungeanum Maxim) collected from Gansu, north-west China. Spores were not observed under a range of conditions. Strain 1404 was observed to grow at 15-45 °C and pH 6.0-10.0 and in presence of 0-5 % (w/v) NaCl concentration. The cell wall of strain 1404 was found to contain meso-diaminopimelic acid, and the predominant respiratory quinone was identified as MK-7. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and an unidentified phospholipid as well as three unidentified polar lipids. The major fatty acids profile of strain 1404 consisted of iso-C15 : 0 (25.6 %), anteiso-C15 : 0 (18.4 %) and iso-C14 : 0 (12.1 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1404 was affiliated to the genus Bacillus and was closely related to Bacillusoryzisoli 1DS3-10 , Bacillusbenzoevorans DSM 5391 and Bacilluscirculans DSM 11 with sequence similarity of 98.3, 98.2 and 96.9 %, respectively. The G+C content of the genomic DNA was determined to be 39.4 mol%. DNA-DNA hybridization values indicated that relatedness between strain 1404 and the type strains of closely related species of the genus Bacillus was below 41 %. Therefore, on the basis of the data from the polyphasic taxonomic study presented, strain 1404 represents a novel species of the genus Bacillus, for which the name proposed is Bacillus endozanthoxylicus sp. nov. The type strain is 1404 (=CCTCC AB 2017021 =KCTC 33827 ).
[Mh] Termos MeSH primário: Bacillus/classificação
Filogenia
Folhas de Planta/microbiologia
Zanthoxylum/microbiologia
[Mh] Termos MeSH secundário: Bacillus/genética
Bacillus/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
Parede Celular/química
China
DNA Bacteriano/genética
Ácido Diaminopimélico/química
Ácidos Graxos/química
Hibridização de Ácido Nucleico
Peptidoglicano/química
Fosfatidilgliceróis/química
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Microbiologia do Solo
Vitamina K 2/análogos & derivados
Vitamina K 2/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Bacterial); 0 (Fatty Acids); 0 (Peptidoglycan); 0 (Phosphatidylglycerols); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S); 11032-49-8 (Vitamin K 2); 583-93-7 (Diaminopimelic Acid); 8427BML8NY (vitamin MK 7)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170908
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002138


  5 / 358 MEDLINE  
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[PMID]:28768929
[Au] Autor:Teshima S; Kawakami S; Sugimoto S; Matsunami K; Otsuka H; Shinzato T
[Ad] Endereço:Graduate School of Biomedical and Health Sciences, Hiroshima University.
[Ti] Título:Aliphatic Glucoside, Zanthoionic Acid and Megastigmane Glucosides: Zanthoionosides A-E from the Leaves of Zanthoxylum ailanthoides.
[So] Source:Chem Pharm Bull (Tokyo);65(8):754-761, 2017.
[Is] ISSN:1347-5223
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:From the leaves of Zanthoxylum ailanthoides, 4'-O-p-E-coumaric acid esters of 2-propanol ß-D-glucopyranoside, megastigmane and megastigmane glucosides were isolated. Their structures were elucidated by spectroscopic evidence. The absolute configurations of the megastigmane and aglycone of megastigmane glucosides were determined by the octant rule and modified Mosher's method after protection of carboxylic acids by p-bromophenacyl esters and primary alcohols by pivaloyl esters.
[Mh] Termos MeSH primário: Cicloexanonas/isolamento & purificação
Glucosídeos/isolamento & purificação
Norisoprenoides/isolamento & purificação
Folhas de Planta/química
Zanthoxylum/química
[Mh] Termos MeSH secundário: Cicloexanonas/química
Glucosídeos/química
Conformação Molecular
Norisoprenoides/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclohexanones); 0 (Glucosides); 0 (Norisoprenoids); 0 (megastigmane); 0 (zanthoionic acid)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170829
[Lr] Data última revisão:
170829
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170804
[St] Status:MEDLINE
[do] DOI:10.1248/cpb.c17-00211


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[PMID]:28716103
[Au] Autor:Alam F; Najum Us Saqib Q; Waheed A
[Ad] Endereço:Department of Pharmacy, COMSATS Institute of Information Technology, Abbottabad, 22060, Pakistan. alamfiaz@ciit.net.pk.
[Ti] Título:Cytotoxic activity of extracts and crude saponins from Zanthoxylum armatum DC. against human breast (MCF-7, MDA-MB-468) and colorectal (Caco-2) cancer cell lines.
[So] Source:BMC Complement Altern Med;17(1):368, 2017 Jul 17.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Zanthoxylum armatum DC has been an important traditional plant known for its medicinal properties. It is well known for its antimicrobial, larvicidal and cytotoxic activities. METHODS: The potential anticancer effects of the methanol extract and the crude saponins from fruit, bark and leaves of Z. armatum on breast (MDA-MB-468 and MCF-7) and colorectal (Caco-2) cancer cell lines using MTT, neutral red uptake(NRU) and DAPI stain assays were evaluated. RESULTS: In MTT assay the methanol extract of fruit (Zf), bark (Zb) and leaves (Zl) of Zanthoxylum armatum, showed significant and dose dependent growth inhibition of MCF-7, MDA MB-468 and Caco-2 cancer cell lines in a dose of 200 µg/ml and above. The saponins (Zf.Sa, Zb.Sa and Zl.Sa) showed significant activity against MDA MB-468 (95, 94.5 and 85.3%) as compared to MCF-7 (79.8, 9.43, 49.08%) and Caco-2 (75.8, 61.8, 68.62%) respectively. The extracts were further tested in more sensitive NRU assay and its was found that Zf extract showed higher cytotoxic activity as compared to Zb and Zl extracts with 100 µg/ml concentration. The breast cancer cell lines showed more sensitivity toward the crude saponins from fruit and bark with maximum inhibition of up to 93.81(±2.32) % with respect to 71.19(± 2.76) of Actinomycin-D. DAPI staining experiment showed that saponins from fruit induced apoptosis mode of cell death in all three types of cell lines while saponins form leaves and bark showed similar results against MDA MB-468 indicated by nuclear fragmentation and chromatin condensation. The effect of saponins from fruit, bark and leaves (Zf.Sa, Zb.Sa and Zl.Sa) against Caco-2 cell lines inhibited the growth of Caco-2 by 53.16 (±3.31) %, 66.43 (± 3.24) and 45.96 (± 10.67) respectively with respect to Actinomycin-D (4 µM) which showed the growth inhibition of 65.40(±4.29) %. CONCLUSION: The current study clearly demonstrates that the extract and crude saponins from fruit, bark and leaves of traditional medicinal plant Zanthoxyllum armatum DC., has the potential to exert its cytotoxic effect on cancer cell lines isolated form human by a mechanism involving apoptosis. The overall finding demonstrate that this plant specially fruits, could be potential source of new anticancer compounds for possible drug development against cancer.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/uso terapêutico
Neoplasias da Mama/tratamento farmacológico
Neoplasias Colorretais/tratamento farmacológico
Fitoterapia
Extratos Vegetais/uso terapêutico
Saponinas/uso terapêutico
Zanthoxylum/química
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/farmacologia
Apoptose
Células CACO-2
Cromatina/efeitos dos fármacos
Fragmentação do DNA
Feminino
Frutas
Seres Humanos
Células MCF-7
Casca de Planta
Extratos Vegetais/farmacologia
Folhas de Planta
Gravidez
Saponinas/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Chromatin); 0 (Plant Extracts); 0 (Saponins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170719
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1882-1


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[PMID]:28583393
[Au] Autor:Han T; Cao X; Xu J; Pei H; Zhang H; Tang Y
[Ad] Endereço:Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University (BTBU), Beijing 100048, China.
[Ti] Título:Separation of the potential G-quadruplex ligands from the butanol extract of Zanthoxylum ailanthoides Sieb. & Zucc. by countercurrent chromatography and preparative high performance liquid chromatography.
[So] Source:J Chromatogr A;1507:104-114, 2017 Jul 21.
[Is] ISSN:1873-3778
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:G-quadruplex DNA structure is considered to be a very attractive target for antitumor drug design due to its unique role in maintaining telomerase activities. Therefore, discovering ligands with high stability of G-quadruplex structure is of great interest. In this paper, pH-zone refining counter current chromatography (CCC) and preparative high performance liquid chromatography (HPLC) were employed for the separation of potent G-quadruplex ligands from the n-butanol fraction of the crude extract of Zanthoxylum ailanthoides, which is a traditional Chinese medicine recently found to display high inhibitory activity against several human cancer cells. The 75% aqueous ethanol extract of the stem bark of Z. ailanthoides and its fractions with petroleum ether, ethyl acetate and n-butanol displayed almost the same G-quadruplex stabilization ability. Here, pH-zone refining CCC was used for the separation of the alkaloids from the n-butanol fraction by a seldom used solvent system composed of dichloromethane-methanol-water (4:1:2.5) with 10mM TEA in the organic stationary phase as retainer and 10mM HCl in the aqueous mobile phase as eluter. Compounds I, II and III were obtained, with purity greater than 95%, in the quantities of 31.2, 94.0, and 26.4mg respectively from 300mg of lipophilic fraction within 80min, which were identified as three tetrahydroprotoberberines isolated for the first time in this plant. In addition, a phenylpropanoid glycoside compound IV (Syringin), an isoquinoline (Magnoflorine, V), and two lignin isomers (+)-lyoniresiol-3α-O-ß-d-glucopyranoside (VI) and (-)-lyoniresinol -3α-O-ß-D -glucopyranoside (VII) were isolated by traditional CCC together with preparative HPLC. Compounds IV, V, VI and VII were obtained, with purity greater than 95%, in the quantities of 4.0, 13.2, 6.7, and 6.5mg respectively from 960mg of hydrophilic fraction. Among the seven isolated compounds, tetrahydroprotoberberine I, II and III were found to display remarkable stabilization effects on G-quadruplex by increasing G-quadruplex's T approximately 10°C, which may be the most potent G-quadruplex ligands in Z. ailanthoides.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Distribuição Contracorrente/métodos
Extratos Vegetais/isolamento & purificação
Zanthoxylum/química
[Mh] Termos MeSH secundário: Alcaloides/química
Alcaloides/isolamento & purificação
Butanóis/química
Seres Humanos
Ligantes
Extratos Vegetais/química
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Alkaloids); 0 (Butanols); 0 (Ligands); 0 (Plant Extracts)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170607
[St] Status:MEDLINE


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[PMID]:28548306
[Au] Autor:Wu HC; Lay IS; Shibu MA; Ho TJ; Cheng SM; Lin CH; Dung TD; Jeng LB; Viswanadha VP; Huang CY
[Ad] Endereço:Department of Urology, China Medical University Hospital, Taichung, 40402, Taiwan.
[Ti] Título:Zanthoxylum avicennae extract enhances GSK-3ß to attenuate ß-catenin via phosphatase 2A to block metastatic effects of HA22T cells and hepatocellular carcinoma xenografted nude mice.
[So] Source:Environ Toxicol;32(9):2133-2143, 2017 Sep.
[Is] ISSN:1522-7278
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Hepatocellular carcinoma (HCC) metastasis is often associated with the activation of Wnt/ß-catenin signaling pathway. Zanthoxylum avicennae (Ying Bu Bo, YBB), a traditional herb with hepatoprotective effect, has been proven to inhibit human HCC in in vivo models however, the in vitro and in vivo effect of YBB on tumor metastasis is not clear yet. To determine whether YBB could inhibit HA22T human HCC cell by acting on ß-catenin metastatic signaling in vitro and in vivo, HA22T cells were treated with different concentrations of YBB extracts (YBBE) and analyzed by Immunofluorescence staining assay, western blot analysis, siRNA mediated gene knock-down assays and co-immunoprecipitation assay. Additionally, the HA22T-implanted xenograft nude mice were used to confirm the assessed cellular effects. Mice treated with YBBEs showed a strong increasing trend in PP2Acα, GSK-3ß, APC, and ß-TrCP/HOS levels, however the expression of ß-catenin, p-GSK-3ß, TBX 3, and IL8 proteins showed a decreasing trend. YBBE significantly downregulated the nuclear and cytosolic ß-catenin levels by facilitating the proteosomal degradation of ß-catenin. Moreover, as observed by co-immunoprecipitation assay, YBBE directly promoted the protein interactions between GSK-3ß, ß-TrCP, APC, PP2A, and ß-catenin. In conclusion, both in vitro and in vivo models clearly demonstrated that YBBE inhibits ß-catenin involved metastatic signaling in highly metastatic HA22T cells through PP2A activation.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Carcinoma Hepatocelular/tratamento farmacológico
Glicogênio Sintase Quinase 3 beta/metabolismo
Neoplasias Hepáticas/tratamento farmacológico
Extratos Vegetais/farmacologia
Proteína Fosfatase 2/metabolismo
Zanthoxylum/química
beta Catenina/metabolismo
[Mh] Termos MeSH secundário: Animais
Antineoplásicos Fitogênicos/uso terapêutico
Carcinoma Hepatocelular/patologia
Linhagem Celular Tumoral
Regulação para Baixo
Quinase 3 da Glicogênio Sintase/metabolismo
Xenoenxertos
Seres Humanos
Neoplasias Hepáticas/patologia
Camundongos Nus
Metástase Neoplásica
Extratos Vegetais/uso terapêutico
Transdução de Sinais
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Plant Extracts); 0 (beta Catenin); EC 2.7.11.1 (Glycogen Synthase Kinase 3 beta); EC 2.7.11.26 (Glycogen Synthase Kinase 3); EC 3.1.3.16 (Protein Phosphatase 2)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170914
[Lr] Data última revisão:
170914
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170527
[St] Status:MEDLINE
[do] DOI:10.1002/tox.22426


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[PMID]:28281719
[Au] Autor:Hong L; Jing W; Qing W; Anxiang S; Mei X; Qin L; Qiuhui H
[Ad] Endereço:College of Food Science and Engineering/Collaborative Innovation Center for Modern Grain Circulation and Safety/Key Laboratory of Grains and Oils Quality Control and Processing, Nanjing University of Finance and Economics, Nanjing 210023, China. leihong56@163.com qiuhuihu@njue.edu.cn.
[Ti] Título:Inhibitory effect of Zanthoxylum bungeanum essential oil (ZBEO) on Escherichia coli and intestinal dysfunction.
[So] Source:Food Funct;8(4):1569-1576, 2017 Apr 19.
[Is] ISSN:2042-650X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The inhibitory effects of Zanthoxylum bungeanum essential oil (ZBEO) on Escherichia coli (E. coli) in vitro and in vivo were investigated, as well as its function of improvement of intestinal health. The results of in vitro studies, such as minimal inhibitory concentration (MIC) analysis, agar disc diffusion test and growth curve analysis of E. coli, showed that ZBEO had an excellent inhibitory effect on the growth of E. coli, which may be related to the loss of the normal shape of the cell membranes and the leakage of intracellular constituents, on the basis of SEM observation and cell constituents' release assay. ZBEO also had an inhibitory effect on enteritis and intestinal dysfunction induced by infection of E. coli in vivo, and histopathological observation indicated that ZBEO could markedly ameliorate the structural destruction of intestinal tissues, which might be related to its inhibitory effect on the gene expression of inflammatory cytokines (TLR2, TLR4, TNF and IL-8). In conclusion, ZBEO showed an excellent inhibitory effect on E. coli both in vitro and in vivo, suggesting the potential application of ZBEO as a kind of functional component having the effects of improving intestinal function and health.
[Mh] Termos MeSH primário: Infecções por Escherichia coli/tratamento farmacológico
Escherichia coli/efeitos dos fármacos
Intestinos/efeitos dos fármacos
Óleos Voláteis/farmacologia
Extratos Vegetais/farmacologia
Zanthoxylum/química
[Mh] Termos MeSH secundário: Animais
Escherichia coli/fisiologia
Infecções por Escherichia coli/metabolismo
Infecções por Escherichia coli/microbiologia
Infecções por Escherichia coli/fisiopatologia
Seres Humanos
Interleucina-8/genética
Interleucina-8/metabolismo
Intestinos/metabolismo
Intestinos/fisiopatologia
Camundongos
Receptor 2 Toll-Like/genética
Receptor 2 Toll-Like/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interleukin-8); 0 (Oils, Volatile); 0 (Plant Extracts); 0 (Toll-Like Receptor 2)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170717
[Lr] Data última revisão:
170717
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170311
[St] Status:MEDLINE
[do] DOI:10.1039/c6fo01739h


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[PMID]:28148252
[Au] Autor:Bunalema L; Fotso GW; Waako P; Tabuti J; Yeboah SO
[Ad] Endereço:Department of Pharmacology and Therapeutics, College of Health Sciences, Makerere University, P.O Box 7072, Kampala, Uganda. blydia@chs.mak.ac.ug.
[Ti] Título:Potential of Zanthoxylum leprieurii as a source of active compounds against drug resistant Mycobacterium tuberculosis.
[So] Source:BMC Complement Altern Med;17(1):89, 2017 Feb 02.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Tuberculosis (TB) is still a global health problem mainly due to development of resistance and co-infection with the Human immune Virus (HIV). Treatment of multi and extensively drug resistant TB requires use of second line drugs which are less efficacious, expensive and very toxic. This has necessitated a need to search for new treatment regimens especially from medicinal plants. Zanthoxylum leprieurii, a plant species from Rutaceae is used locally in the treatment of tuberculosis in Uganda. The aim of the study was to isolate, identify and characterize bio active compounds from Z. leprieurii stem bark with antimycobacterial activity. METHODS: Crude extracts, fractions and compounds from air dried stem bark of Z. leprieurii were tested against pan sensitive (H37rv), isoniazid resistant (TMC 301) and rifampicin resistant (TMC 331) strains of M. tuberculosis using micro plate alamar blue assay. Isolation of active compounds was done by using column chromatography and thin layer chromatography. They were analysed using nuclear magnetic resonance spectroscopy and mass spectroscopy. RESULTS: The methanol extract had minimum inhibitory concentrations (MIC) of 47.5, 75.3 and 125.0 µg/ml on the pan sensitive strain, rifampicin resistant and isozianid resistant strains of M. tuberculosis respectively. The chloroform extract had MIC values of 260 µg/ml agnaist the pan sensitive strain and 156 µg/ml on the rifampicin resistant strain. Of the sixteen fractions from the methanol extract, fraction Za (MIC = 6.3 µg/mL, 23.0 µg/mL, 11.7 µg/mL) and Za (MIC = 11.7 µg/mL 31.2 µg/ml, 31.2 µg/ml) were the most active. Three acridone alkaloids; hydroxy-1, 3-dimethoxy-10-methyl-9-acridone (1), 1-hydroxy-3-methoxy-10-methyl-9-acridone (2) and 3-hydroxy-1, 5, 6-trimethoxy-9-acridone (3) were isolated from Za and Za . The MIC of compound 3 was found to be 5.1 µg/ml, 4.5 µg/ml and 3.9 µg/ml on H37rv, TMC 331 and TMC 301 while that of 1 was found to be 1.5 µg/ml, 8.3 µg/ml and 3.5 µg/ml respectively. CONCLUSION: The results of this study suggest that Z. leprieurii is active on resistant strains of M. tuberculosis and could be a potential source of new leads against resistant tuberculosis. It also verifies the local use of the plant in treatment of tuberculosis.
[Mh] Termos MeSH primário: Antituberculosos/farmacologia
Mycobacterium tuberculosis/efeitos dos fármacos
Extratos Vegetais/farmacologia
Zanthoxylum/química
[Mh] Termos MeSH secundário: Farmacorresistência Bacteriana
Testes de Sensibilidade Microbiana
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antitubercular Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170203
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1602-x



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