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| [PMID]: | 23041381 |
| [Au] Autor: | Su HJ; Hu JM |
| [Ad] Endereço: | Institute of Ecology and Evolutionary Biology, National Taiwan University, Taiwan. |
| [Ti] Título: | Rate heterogeneity in six protein-coding genes from the holoparasite Balanophora (Balanophoraceae) and other taxa of Santalales. |
| [So] Source: | Ann Bot;110(6):1137-47, 2012 Nov. | | [Is] ISSN: | 1095-8290 |
| [Cp] País de publicação: | England |
| [La] Idioma: | eng |
| [Ab] Resumo: | BACKGROUND AND AIMS: The holoparasitic flowering plant Balanophora displays extreme floral reduction and was previously found to have enormous rate acceleration in the nuclear 18S rDNA region. So far, it remains unclear whether non-ribosomal, protein-coding genes of Balanophora also evolve in an accelerated fashion and whether the genes with high substitution rates retain their functionality. To tackle these issues, six different genes were sequenced from two Balanophora species and their rate variation and expression patterns were examined. METHODS: Sequences including nuclear PI, euAP3, TM6, LFY and RPB2 and mitochondrial matR were determined from two Balanophora spp. and compared with selected hemiparasitic species of Santalales and autotrophic core eudicots. Gene expression was detected for the six protein-coding genes and the expression patterns of the three B-class genes (PI, AP3 and TM6) were further examined across different organs of B. laxiflora using RT-PCR. KEY RESULTS: Balanophora mitochondrial matR is highly accelerated in both nonsynonymous (d(N)) and synonymous (d(S)) substitution rates, whereas the rate variation of nuclear genes LFY, PI, euAP3, TM6 and RPB2 are less dramatic. Significant d(S) increases were detected in Balanophora PI, TM6, RPB2 and d(N) accelerations in euAP3. All of the protein-coding genes are expressed in inflorescences, indicative of their functionality. PI is restrictively expressed in tepals, synandria and floral bracts, whereas AP3 and TM6 are widely expressed in both male and female inflorescences. CONCLUSIONS: Despite the observation that rates of sequence evolution are generally higher in Balanophora than in hemiparasitic species of Santalales and autotrophic core eudicots, the five nuclear protein-coding genes are functional and are evolving at a much slower rate than 18S rDNA. The mechanism or mechanisms responsible for rapid sequence evolution and concomitant rate acceleration for 18S rDNA and matR are currently not well understood and require further study in Balanophora and other holoparasites. |
| [Mh] Termos MeSH primário: |
Balanophoraceae/genética
Evolução Molecular
Flores/genética
Filogenia
Proteínas de Plantas/genética
Santalaceae/genética
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| [Mh] Termos MeSH secundário: |
Balanophoraceae/ultraestrutura
DNA Mitocondrial/química
DNA Mitocondrial/genética
DNA de Plantas/química
DNA de Plantas/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Flores/ultraestrutura
Inflorescência/genética
Inflorescência/ultraestrutura
Microscopia Eletrônica de Varredura
Mitocôndrias/genética
Proteínas Nucleares/genética
Epiderme Vegetal/genética
Epiderme Vegetal/ultraestrutura
RNA Ribossômico 18S/genética
Santalaceae/ultraestrutura
Análise de Sequência de DNA
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| [Pt] Tipo de publicação: | JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T |
[Nm] Nome de substância:
| 0 (DNA, Mitochondrial); 0 (DNA, Plant); 0 (DNA, Ribosomal); 0 (Nuclear Proteins); 0 (Plant Proteins); 0 (RNA, Ribosomal, 18S) |
| [Em] Mês de entrada: | 1304 |
| [Cu] Atualização por classe: | 170220 |
[Lr] Data última revisão:
| 170220 |
| [Sb] Subgrupo de revista: | IM |
| [Da] Data de entrada para processamento: | 121009 |
| [St] Status: | MEDLINE |
| [do] DOI: | 10.1093/aob/mcs197 |
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