Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.885 [Categoria DeCS]
Referências encontradas : 385 [refinar]
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[PMID]:29381727
[Au] Autor:Li H; Lin Y; Chen X; Bai Y; Wang C; Xu X; Wang Y; Lai Z
[Ad] Endereço:Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian, China.
[Ti] Título:Effects of blue light on flavonoid accumulation linked to the expression of miR393, miR394 and miR395 in longan embryogenic calli.
[So] Source:PLoS One;13(1):e0191444, 2018.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:While flavonoid metabolism's regulation under light conditions by structural genes and transcription factors is understood, the roles of microRNAs (miRNAs) in this pathway have been rarely reported. In this paper, the accurate control of light was firstly enabled through the specially designed plant growth chamber which ensures consistency and accuracy of the cultivation of longan ECs and the repeatability of the experiments. Then, longan ECs were cultured in this chamber for 25 days. The change of growth rate of longan ECs was compared under different light qualities (dark, blue, green, white, green), intensities (16, 32, 64, 128, 256 µmol ·m-2 ·s-1), and durations (8 h, 12 h, 16 h, 20h, 24h). Results indicated that longan ECs had a high growth rate in the condition of blue or green light, at intensity ranged from 16 µmol·m-2·s-1 to 64 µmol·m-2·s-1, and duration from 8 h to 16 h. In addition, the contents of total flavonoids, rutin, and epicatechin were determined. Results indicated that flavonoid contents of longan ECs reached the highest value under blue light, at 32 µmol·m-2·s-1 and 12h/d. Blue light promoted the accumulation of epicatechin, but inhibited the synthesis of rutin. Finally, the expressions of flavonoid pathway genes, miRNAs and target genes were analyzed by qPCR. These results indicated that miR393 and its target gene DlTIR1-3, miR394 and its target gene DlAlMT12, and miR395 and its target gene DlAPS1 had a negative regulating relationship under blue light in longan ECs. Furthermore, miR393, miR394, and miR395 acted on target genes, which negatively regulated flavonoid key genes DlFLS and positively regulated key genes DlCHS, DlCHI, DlF3'H, DlDFR, DlLAR, and finally affected the accumulation of flavonoids. The treatment of longan ECs under the blue light at the intensity of 32 µmol·m-2·s-1 for 12 h/d inhibited the expression of miR393, miR394 and miR395, which promoted the expression of target genes and the accumulation of flavonoids and epicatechin, but inhibited the synthesis of rutin.
[Mh] Termos MeSH primário: Regulação da Expressão Gênica de Plantas/efeitos da radiação
Luz
MicroRNAs/genética
Sapindaceae/metabolismo
Sapindaceae/efeitos da radiação
[Mh] Termos MeSH secundário: Relação Dose-Resposta à Radiação
Fotoperíodo
Sapindaceae/citologia
Sapindaceae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (MicroRNAs)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180226
[Lr] Data última revisão:
180226
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180131
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0191444


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[PMID]:29313327
[Au] Autor:Chai WM; Huang Q; Lin MZ; Ou-Yang C; Huang WY; Wang YX; Xu KL; Feng HL
[Ad] Endereço:College of Life Science and Key Laboratory of Small Functional Organic Molecule, Ministry of Education, Jiangxi Normal University , Nanchang, Jiangxi 330022, People's Republic of China.
[Ti] Título:Condensed Tannins from Longan Bark as Inhibitor of Tyrosinase: Structure, Activity, and Mechanism.
[So] Source:J Agric Food Chem;66(4):908-917, 2018 Jan 31.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, the content, structure, antityrosinase activity, and mechanism of longan bark condensed tannins were evaluated. The findings obtained from mass spectrometry demonstrated that longan bark condensed tannins were mixtures of procyanidins, propelargonidins, prodelphinidins, and their acyl derivatives (galloyl and p-hydroxybenzoate). The enzyme analysis indicated that these mixtures were efficient, reversible, and mixed (competitive is dominant) inhibitor of tyrosinase. What's more, the mixtures showed good inhibitions on proliferation, intracellular enzyme activity and melanogenesis of mouse melanoma cells (B ). From molecular docking, the results showed the interactions between inhibitors and tyrosinase were driven by hydrogen bond, electrostatic, and hydrophobic interactions. In addition, high levels of total phenolic and extractable condensed tannins suggested that longan bark might be a good source of tyrosinase inhibitor. This study would offer theoretical basis for the development of longan bark condensed tannins as novel food preservatives and medicines of skin diseases.
[Mh] Termos MeSH primário: Inibidores Enzimáticos/farmacologia
Monofenol Mono-Oxigenase/antagonistas & inibidores
Casca de Planta/química
Sapindaceae/química
Taninos/química
Taninos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antocianinas/farmacologia
Biflavonoides/farmacologia
Catequina/farmacologia
Proliferação Celular/efeitos dos fármacos
Ligações de Hidrogênio
Interações Hidrofóbicas e Hidrofílicas
Espectrometria de Massas
Melaninas/análise
Melaninas/antagonistas & inibidores
Melaninas/biossíntese
Melanoma Experimental
Camundongos
Modelos Moleculares
Simulação de Acoplamento Molecular
Oxirredutases
Parabenos/farmacologia
Proantocianidinas/farmacologia
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
Eletricidade Estática
Relação Estrutura-Atividade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Biflavonoids); 0 (Enzyme Inhibitors); 0 (Melanins); 0 (Parabens); 0 (Proanthocyanidins); 0 (Tannins); 4852-22-6 (procyanidin); 8R1V1STN48 (Catechin); EC 1.- (Oxidoreductases); EC 1.14.18.- (monophenolase); EC 1.14.18.1 (Monophenol Monooxygenase); EM6MD4AEHE (delphinidin); JG8Z55Y12H (4-hydroxybenzoic acid)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180110
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b05481


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[PMID]:28638876
[Au] Autor:Fall AD; Bagla VP; Bassene E; Eloff JN
[Ad] Endereço:Phytomedicine Programme, Department of Paraclinical Sciences, Faculty of Veterinary Science, University of Pretoria, Private Bag X04, Onderstepoort, 0110 Pretoria, South Africa.
[Ti] Título:PHYTOCHEMICAL SCREENING, ANTIMICROBIAL AND CYTOTOXICITY STUDIES OF ETHANOL LEAF EXTRACT OF ( ).
[So] Source:Afr J Tradit Complement Altern Med;14(4):135-139, 2017.
[Is] ISSN:2505-0044
[Cp] País de publicação:Nigeria
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: is commonly used in Senegalese traditional medicine to treat pain, inflammation, asthenia, bacterial and fungal infections. The aim of this study was to determine the type of phytochemical constituents present in the ethanol leaf extract and its antimicrobial activity against selected bacterial and fungal pathogens. MATERIALS AND METHODS: The ethanol leaf extract of . was evaluated for its cytotoxic effect in the MTT assay against Vero cells. Flavonoids and tannins were the main constituents of the ethanol leaf extract. RESULTS: The extract inhibited the growth of the three fungal strains used in this study moderately with the lowest MIC obtained for (0.16 mg/mL). The extract also inhibited the growth of and with an MIC of 0.62 mg/mL. For bacterial pathogens, strong inhibition was obtained against (ATTC 29212) (MIC 0.08 mg/mL), while moderate inhibition was obtained for (ATTC 25922) (MIC 0.16 mg/mL) and (ATTC 29213) (MIC 0.31mg/mL). The extract however did not inhibit the growth of (ATTC 27853) at the highest concentration (2.5 mg/ml) tested. The ethanol leaf extract of had a higher cytotoxicity than berberine used as the positive control (LC ±0.04 µg/mL and 9.99±0.54 µg/mL respectively). The best selectivity index values was obtained for (SI = 1.24), followed by (SI = 0.62) for bacterial pathogens and (SI = 0.62) for fungal pathogens. CONCLUSION: The findings of this study suggest that the extracts may not be safe for use in animals infected by some pathogens.
[Mh] Termos MeSH primário: Anti-Infecciosos/química
Anti-Infecciosos/farmacologia
Extratos Vegetais/química
Extratos Vegetais/farmacologia
Sapindaceae/química
[Mh] Termos MeSH secundário: Animais
Anti-Infecciosos/isolamento & purificação
Bactérias/efeitos dos fármacos
Bactérias/crescimento & desenvolvimento
Sobrevivência Celular/efeitos dos fármacos
Cercopithecus aethiops
Flavonoides/análise
Flavonoides/farmacologia
Fungos/efeitos dos fármacos
Fungos/crescimento & desenvolvimento
Testes de Sensibilidade Microbiana
Extratos Vegetais/isolamento & purificação
Folhas de Planta/química
Taninos/análise
Taninos/farmacologia
Células Vero
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Flavonoids); 0 (Plant Extracts); 0 (Tannins)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170623
[St] Status:MEDLINE
[do] DOI:10.21010/ajtcam.v14i4.16


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[PMID]:28609973
[Au] Autor:Casari SA; Albertoni FF
[Ad] Endereço:Museu de Zoologia da Universidade de São Paulo, Caixa Postal 42494; 04218-970, São Paulo, São Paulo BRAZIL. casari@usp.br.
[Ti] Título:Immatures of Cerambycinae (Coleoptera, Cerambycidae) collected in Litchi chinensis Sonn. (Sapindaceae) in Brazil.
[So] Source:Zootaxa;4254(2):240-254, 2017 Apr 12.
[Is] ISSN:1175-5334
[Cp] País de publicação:New Zealand
[La] Idioma:eng
[Ab] Resumo:Larvae and pupae of Coleoxestia waterhousei (Gounelle, 1909) (Cerambycini: Sphallotrichina) and Retrachydes thoracicus (Olivier, 1790) (Trachyderini: Trachyderina), collected in lychee trees, are described and illustrated. This is the first record of Litchi chinensis Sonn. as a host plant Retrachydes thoracicus.
[Mh] Termos MeSH primário: Coleópteros
[Mh] Termos MeSH secundário: Animais
Brasil
Frutas
Litchi
Sapindaceae
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1708
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170615
[St] Status:MEDLINE
[do] DOI:10.11646/zootaxa.4254.2.5


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[PMID]:28570701
[Au] Autor:Shen Z; Duan J; Ma L
[Ad] Endereço:National Energy R&D Center for Non-food Biomass, Beijing Forestry University, Beijing, China.
[Ti] Título:Genetic diversity of Xanthoceras sorbifolium bunge germplasm using morphological traits and microsatellite molecular markers.
[So] Source:PLoS One;12(6):e0177577, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Xanthoceras sorbifolium Bunge has great potential for producing biodiesel. In order to select and evaluate appropriate germplasm to produce biodiesel, we analyzed the genetic diversity of Xanthoceras sorbifolium Bunge germplasm based on morphological traits and simple sequence repeats (SSRs) in this study. Fifty-six germplasm samples were evaluated using nine morphological traits and 23 SSR loci. Significant differences among germplasms were observed in eight morphological characters. The SSR markers analysis showed high genetic diversity among the germplasms. All SSRs had polymorphisms, and we detected 77 alleles in total. The number of alleles at each locus ranged from two to six, averaging 3.35 per marker. The polymorphic information content values ranged from 0.36 to 0.61, averaging 0.49. Expected heterozygosity, observed heterozygosity, and Shannon's information index calculations detected large genetic variations among germplasms. The high average number of alleles per locus and the allelic diversity observed in the set of genotypes analyzed indicated that the genetic base of this species is relatively wide. Thus, microsatellite markers can be used to efficiently distinguish Xanthoceras sorbifolium Bunge germplasms and assess their genetic diversity. Hundred-grain weight and lateral diameter were positively correlated with monounsaturated fatty acids and depended on genotype. These results suggest that seeds with higher hundred-grain weight and lateral diameter could be more suitable to produce biodiesel. Our data will lay a foundation for selecting appropriate germplasm to produce biodiesel based on seed phenotype and will contribute to the conservation and management of this important plant genetic resource.
[Mh] Termos MeSH primário: Marcadores Genéticos
Repetições de Microssatélites/genética
Sapindaceae/genética
[Mh] Termos MeSH secundário: Análise por Conglomerados
Genes de Plantas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Genetic Markers)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170921
[Lr] Data última revisão:
170921
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170602
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0177577


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[PMID]:28394604
[Au] Autor:Jin Y; Fan JT; Gu XL; Zhang LY; Han J; Du SH; Zhang AX
[Ti] Título:Neuroprotective Activity of Cerebrosides from Typhonium giganteum by Regulating Caspase-3 and Bax/Bcl-2 Signaling Pathways in PC12 Cells.
[So] Source:J Nat Prod;80(6):1734-1741, 2017 Jun 23.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:An investigation of the potential neuroprotective natural product constituents of the rhizomes of Typhonium giganteum led to the isolation of two new cerebrosides, typhonosides E (1) and F (2), along with 11 known analogues (3-13). The structures of compounds 1 and 2 were elucidated by spectroscopic data interpretation. The activity of these compounds against glutamate-induced cell apoptosis was investigated in PC12 cells. All compounds exhibited such activity, which was related to the length of the fatty acyl chain. Among them, longan cerebroside II (11), with the longest fatty acyl chain, showed the most potent protective effect in PC12 cells from glutamate injury, with an EC value of 2.5 µM. Moreover, at the molecular level, longan cerebroside II (11) downregulated the expression of caspase-9, caspase-3, and Bax, upregulated the expression of Bcl-2, and decreased the level of cytosolic cytochrome c in a concentration-dependent manner.
[Mh] Termos MeSH primário: Cerebrosídeos/isolamento & purificação
Cerebrosídeos/farmacologia
Fármacos Neuroprotetores/isolamento & purificação
Fármacos Neuroprotetores/farmacologia
[Mh] Termos MeSH secundário: Animais
Apoptose/efeitos dos fármacos
Caspase 3/metabolismo
Caspase 9/metabolismo
Cerebrosídeos/química
Citocromos c/metabolismo
Ácido Glutâmico/farmacologia
Estrutura Molecular
Fármacos Neuroprotetores/química
Células PC12
Proteínas Proto-Oncogênicas c-bcl-2/metabolismo
Ratos
Rizoma/química
Sapindaceae
Transdução de Sinais/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cerebrosides); 0 (Neuroprotective Agents); 0 (Proto-Oncogene Proteins c-bcl-2); 0 (typhonoside); 0 (typhonoside E); 0 (typhonoside F); 3KX376GY7L (Glutamic Acid); 9007-43-6 (Cytochromes c); EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 9)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170830
[Lr] Data última revisão:
170830
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170411
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b00954


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[PMID]:28379211
[Au] Autor:Chen C; Lu RS; Zhu SS; Tamaki I; Qiu YX
[Ad] Endereço:Key Laboratory of Conservation Biology for Endangered Wildlife of the Ministry of Education, and College of Life Sciences, Zhejiang University, Hangzhou, People's Republic of China.
[Ti] Título:Population structure and historical demography of Dipteronia dyeriana (Sapindaceae), an extremely narrow palaeoendemic plant from China: implications for conservation in a biodiversity hot spot.
[So] Source:Heredity (Edinb);119(2):95-106, 2017 Aug.
[Is] ISSN:1365-2540
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Inferring past demography is a central question in evolutionary and conservation biology. It is, however, sometimes challenging to disentangle their roles of contemporary versus historical processes in shaping the current patterns of genetic variation in endangered species. In this study, we used both chloroplast microsatellite (cpSSR) loci and nuclear microsatellite (nSSR) loci to assess the levels of genetic differentiation, genetic effective population size, contemporary/historical levels of gene flow and demographic history for five populations sampled across the range of Dipteronia dyeriana, an endangered palaeoendemism from Southwestern China. We found that D. dyeriana had a mixed pattern of moderate genetic diversity and high inbreeding. Bayesian clustering divided D. dyeriana populations into two nSSR genetic clusters. Coalescent-based approximate Bayesian computation analyses suggest the western and eastern groups of D. dyeriana likely persisted in a long-term refuge in Southern China since the beginning of the last glacial period, whereas increasingly colder and arid climates at the onset of the last glacial maximum might have fostered the fragmentation of D. dyeriana within refugia. Following their divergence, the western group kept relatively stable effective population size, whereas the eastern group had experienced 500-fold population expansion during the Holocene. Although clear loss of genetic diversity by human activities was not suggested, recent habitat fragmentation has led to a reduction of population connectivity and increased genetic differentiation by ongoing genetic drift in isolated populations, possibly owing to decreased population size in recent dozen years. Finally, we discussed the implications of these results on conservation policies.
[Mh] Termos MeSH primário: Fluxo Gênico
Variação Genética
Genética Populacional
Sapindaceae/genética
[Mh] Termos MeSH secundário: Teorema de Bayes
Biodiversidade
China
Conservação dos Recursos Naturais
DNA de Cloroplastos/genética
DNA de Plantas/genética
Espécies em Perigo de Extinção
Deriva Genética
Endogamia
Repetições de Microssatélites
Densidade Demográfica
Dinâmica Populacional
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Chloroplast); 0 (DNA, Plant)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170406
[St] Status:MEDLINE
[do] DOI:10.1038/hdy.2017.19


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[PMID]:28299408
[Au] Autor:Zheng S; Pan T; Ma C; Qiu D
[Ad] Endereço:College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou, 350002, China.
[Ti] Título:Differential Gene Expression of Longan Under Simulated Acid Rain Stress.
[So] Source:Bull Environ Contam Toxicol;98(5):726-731, 2017 May.
[Is] ISSN:1432-0800
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Differential gene expression profile was studied in Dimocarpus longan Lour. in response to treatments of simulated acid rain with pH 2.5, 3.5, and a control (pH 5.6) using differential display reverse transcription polymerase chain reaction (DDRT-PCR). Results showed that mRNA differential display conditions were optimized to find an expressed sequence tag (EST) related with acid rain stress. The potential encoding products had 80% similarity with a transcription initiation factor IIF of Gossypium raimondii and 81% similarity with a protein product of Theobroma cacao. This fragment is the transcription factor activated by second messenger substances in longan leaves after signal perception of acid rain.
[Mh] Termos MeSH primário: Chuva Ácida
Perfilação da Expressão Gênica
Folhas de Planta/genética
Sapindaceae/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sequência de Bases
RNA de Plantas/isolamento & purificação
Análise de Sequência de DNA
Estresse Fisiológico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acid Rain); 0 (RNA, Plant)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170609
[Lr] Data última revisão:
170609
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170317
[St] Status:MEDLINE
[do] DOI:10.1007/s00128-017-2059-9


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[PMID]:28238828
[Au] Autor:de Oliveira RG; de Campos Castilho GR; da Cunha AL; Miyajima F; de Oliveira Martins DT
[Ad] Endereço:Department of Basic Health Sciences, Faculty of Medicine, Federal University of Mato Grosso (UFMT), Av. Fernando Correa da Costa, no. 2367, Coxipó, Cuiabá, Mato Grosso 78060-900, Brazil; Faculdade de Farmácia, Universidade de Cuiabá, Avenida Beira Rio, no. 3100, CEP: 78025-190, Cuiabá, MT, Brazil.
[Ti] Título:Dilodendron bipinnatum Radlk. inhibits pro-inflammatory mediators through the induction of MKP-1 and the down-regulation of MAPKp38/JNK/NF-κB pathways and COX-2 in LPS-activated RAW 264.7 cells.
[So] Source:J Ethnopharmacol;202:127-137, 2017 Apr 18.
[Is] ISSN:1872-7573
[Cp] País de publicação:Ireland
[La] Idioma:eng
[Ab] Resumo:ETHNOPHARMACOLOGICAL RELEVANCE: The stem bark of Dilodendron bipinnatum Radlk. (Sapindaceae), a tree native to Pantanal of Mato Grosso, Brazil, popularly known as "mulher-pobre" (poor woman), has been historically used by the locals, after decoction and maceration, for the treatment of inflammatory conditions. We have recently shown that these preparations indeed possess anti-inflammatory properties, which are mediated by the inhibition of cell migration and the modulation of Th1 and Th2 cytokines. The NO pathway was not affected. AIM OF THE PRESENT STUDY: The aim of the present study was to further investigate the mechanisms responsible for the anti-inflammatory properties of the hydroethanolic extract of the stem bark of Dilodendron bipinnatum (HEDb). MATERIALS AND METHODS: HEDb was obtained by maceration of the stem bark of D. bipinnatum as previously described. The corresponding effects on a macrophage-like cell line, RAW 264.7, were investigated. The apoptosis of RAW 264.7 upon treatment with LPS, HEDb and N-acetyl-L-cysteine (NAC) was assessed by flow cytometry, using an Annexin V-PE kit. The production of inflammatory cytokines (TNF-α, IL-1ß and IL-10) and PGE were evaluated by ELISA, after cell challenge with LPS. The intracellular redox state and changes in mitochondrial membrane potential were also assessed by flow cytometry, using DCFH-DA and JC-1 as probes. The protein expression levels of MAPK p-p38, p-ERK, p-JNK, MKP-1 and COX-2 were analysed by western blotting. Nuclear translocation of NF-Ò›B was assessed by immunofluorescence microscopy. The quantified results are presented as a nuclear:cytoplasmic ratio. RESULTS: LPS, HEDb and NAC did not appear to decrease the number of viable cells in comparison to control treatment. HEDb attenuated the production of pro-inflammatory cytokines (IL-1ß and TNF-α) and PGE induced by LPS but did not affect IL-10. The production of ROS was also inhibited by HEDb (1, 5 or 20µg/mL), even at the lowest concentration; at 20µg/mL, HEDb was more effective than NAC, which was used as a positive control (74.1% and 66.2% inhibition of LPS's effect, respectively). LPS induced an increase in ΔΨm (19.2%, p<0.001), while HEDb inhibited the change in ΔΨm (7.7% at 20µg/mL, p<0.001). The results of western blotting showed that HEDb inhibited the expression of MAPK p-p38, p-JNK and COX-2, while the expression of MKP-1 was increased. p-ERK was not affected. LPS promoted the nuclear translocation of NF-Ò›B p65 (67%, p<0.01) in RAW 264.7 cells, in comparison to baseline (33%). Pre-treatment with HEDb inhibited this translocation of NF-κB p65 (58% at 20µg/mL, p<0.001). CONCLUSION: HEDb has a potent anti-inflammatory activity and acts on multiple targets and biological pathways of potential therapeutic relevance.
[Mh] Termos MeSH primário: Anti-Inflamatórios não Esteroides/farmacologia
Mediadores da Inflamação/antagonistas & inibidores
Extratos Vegetais/farmacologia
Sapindaceae/química
[Mh] Termos MeSH secundário: Animais
Antioxidantes/farmacologia
Brasil
Citocinas/biossíntese
Dinoprostona/biossíntese
Seres Humanos
Lipopolissacarídeos/antagonistas & inibidores
Lipopolissacarídeos/farmacologia
Medicina Tradicional
Potencial da Membrana Mitocondrial/efeitos dos fármacos
Camundongos
Estresse Oxidativo/efeitos dos fármacos
Casca de Planta/química
Caules de Planta/química
Células RAW 264.7
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents, Non-Steroidal); 0 (Antioxidants); 0 (Cytokines); 0 (Inflammation Mediators); 0 (Lipopolysaccharides); 0 (Plant Extracts); K7Q1JQR04M (Dinoprostone)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170824
[Lr] Data última revisão:
170824
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170228
[St] Status:MEDLINE


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[PMID]:28193430
[Au] Autor:Lin Y; Lin Y; Lin H; Ritenour MA; Shi J; Zhang S; Chen Y; Wang H
[Ad] Endereço:Institute of Postharvest Technology of Agricultural Products, College of Food Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China; Indian River Research & Education Center, Horticultural Sciences Department, University of Florida, Fort Pierce 34945, USA.
[Ti] Título:Hydrogen peroxide-induced pericarp browning of harvested longan fruit in association with energy metabolism.
[So] Source:Food Chem;225:31-36, 2017 Jun 15.
[Is] ISSN:0308-8146
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Energy metabolism of "Fuyan" longan fruit treated with hydrogen peroxide (H O ), the most stable of the reactive oxygen, and its relationship to pericarp browning were investigated in this work. The results displayed that H O significantly decreased contents of adenosine triphosphate (ATP) and adenosine diphosphate (ADP). It also inhibited activities of H -ATPase, Ca -ATPase and Mg -ATPase in membranes of plasma, vacuole and mitochondria during the early-storage and mid-storage (except for mitochondrial membrane Mg -ATPase). These results gave convincing evidence that the treatment of H O accelerating pericarp browning in harvested longans was due to a decrease of ATPase activity and available ATP content. This might break the ion homeostasis and the integrity of mitochondria, which might reduce energy charge and destroy the function and compartmentalization of cell membrane. These together aggravated browning incidence in pericarp of harvested longan fruit.
[Mh] Termos MeSH primário: Metabolismo Energético/efeitos dos fármacos
Frutas/metabolismo
Peróxido de Hidrogênio/farmacologia
Sapindaceae/metabolismo
[Mh] Termos MeSH secundário: Difosfato de Adenosina/análise
Difosfato de Adenosina/metabolismo
Trifosfato de Adenosina/análise
Trifosfato de Adenosina/metabolismo
Frutas/química
Mitocôndrias/efeitos dos fármacos
Mitocôndrias/metabolismo
Sapindaceae/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
61D2G4IYVH (Adenosine Diphosphate); 8L70Q75FXE (Adenosine Triphosphate); BBX060AN9V (Hydrogen Peroxide)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170504
[Lr] Data última revisão:
170504
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170215
[St] Status:MEDLINE



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