Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.905.499 [Categoria DeCS]
Referências encontradas : 212 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 22 ir para página                         

  1 / 212 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28774732
[Au] Autor:Ma T; Zhang Y; Zhang C; Luo JG; Kong LY
[Ad] Endereço:State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing 210009, China.
[Ti] Título:Downregulation of TIGAR sensitizes the antitumor effect of physapubenolide through increasing intracellular ROS levels to trigger apoptosis and autophagosome formation in human breast carcinoma cells.
[So] Source:Biochem Pharmacol;143:90-106, 2017 Nov 01.
[Is] ISSN:1873-2968
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Physapubenolide (PB) is a cytotoxic withanolide isolated from Physalis angulata that was used as a traditional Chinese medicine. In this study, we investigated the role of TIGAR and ROS in PB-induced apoptosis and autophagosome formation in human breast carcinoma MDA-MB-231 and MCF-7 cells. PB induced apoptosis by decreasing mitochondrial membrane potential and elevating the Bax/Bcl-2 protein expression ratio in MDA-MB-231 and MCF-7 cells. Caspase inhibitor Z-VAD-FMK treatment partly blocked PB induced cytotoxicity, suggesting that apoptosis serves as an important role in the anti-proliferative effect of PB. Meanwhile, PB induced autophagosome formation, as characterized by increased acridine orange-stained positive cells, accumulation of punctate LC3B fluorescence and a greater number of autophagic vacuoles under electron microscopy. Furthermore, PB inhibited autophagic flux as reflected by the overlapping of mCherry and GFP fluorescence when MDA-MB-231 cells were transfected with GFP-mCherry-LC3 plasmid. Depletion of LC3B, ATG5 or ATG7 reduced PB-induced cytotoxicity, indicating that autophagosome associated cell death participated in the anti-cancer effect of PB. Moreover, PB-induced apoptosis and autophagosome formation were linked to the generation of intracellular ROS, and pre-treatment with the antioxidant NAC obviously mitigated the effects. Interestingly, PB treatment slightly increased TIGAR expression at low concentrations but decreased TIGAR expression drastically at high concentrations. Downregulation of TIGAR by small interfering RNA augmented low concentrations of PB-induced apoptosis and autophagosome formation, which contributed to the observed anti-cancer effect of PB and were reversed by NAC pre-treatment. Consistently, in MDA-MB-231 or MCF-7 xenograft mouse model, PB suppressed tumor growth through ROS induced apoptosis and autophagosome associated cell death accompanied with the downregulation of TIGAR. Taken together, these results indicate that downregulation of TIGAR increased PB-induced apoptosis and autophagosomes associated cell death through promoting ROS generation in MDA-MB-231 and MCF-7 cells.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/farmacologia
Apoptose/efeitos dos fármacos
Autofagossomos/efeitos dos fármacos
Peptídeos e Proteínas de Sinalização Intracelular/genética
Espécies Reativas de Oxigênio/metabolismo
Vitanolídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Antineoplásicos Fitogênicos/isolamento & purificação
Autofagia/efeitos dos fármacos
Neoplasias da Mama/metabolismo
Neoplasias da Mama/patologia
Proliferação Celular/efeitos dos fármacos
Sobrevivência Celular/efeitos dos fármacos
Relação Dose-Resposta a Droga
Regulação para Baixo
Feminino
Citometria de Fluxo
Seres Humanos
Células MCF-7
Camundongos Endogâmicos BALB C
Camundongos Nus
Physalis/química
RNA Interferente Pequeno/genética
Ensaio Tumoral de Célula-Tronco
Vitanolídeos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (C12orf5 protein, human); 0 (Intracellular Signaling Peptides and Proteins); 0 (RNA, Small Interfering); 0 (Reactive Oxygen Species); 0 (Withanolides); 0 (physapubenolide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170805
[St] Status:MEDLINE


  2 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28696106
[Au] Autor:Wen X; Hempel J; Schweiggert RM; Ni Y; Carle R
[Ad] Endereço:Institute of Food Science and Biotechnology, University of Hohenheim , 70599 Stuttgart, Germany.
[Ti] Título:Carotenoids and Carotenoid Esters of Red and Yellow Physalis (Physalis alkekengi L. and P. pubescens L.) Fruits and Calyces.
[So] Source:J Agric Food Chem;65(30):6140-6151, 2017 Aug 02.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Carotenoid profiles of fruits and calyces of red (Physalis alkekengi L.) and yellow (P. pubescens L.) Physalis were characterized by HPLC-DAD-APCI-MS . Altogether 69 carotenoids were detected in red Physalis, thereof, 45 were identified. In yellow Physalis, 40 carotenoids were detected and 33 were identified. Zeaxanthin esters with various fatty acids were found to be the most abundant carotenoids in red Physalis, accounting for 51-63% of total carotenoids, followed by ß-cryptoxanthin esters (16-24%). In yellow Physalis, mainly free carotenoids such as lutein and ß-carotene were found. Total carotenoid contents ranged between 19.8 and 21.6 mg/100 g fresh red Physalis fruits and 1.28-1.38 mg/100 g fresh yellow Physalis fruits, demonstrating that Physalis fruits are rich sources of dietary carotenoids. Yellow Physalis calyces contained only 153-306 µg carotenoids/g dry weight, while those of red Physalis contained substantially higher amounts (14.6-17.6 mg/g dry weight), thus possibly exhibiting great potential as a natural source for commercial zeaxanthin extraction.
[Mh] Termos MeSH primário: Carotenoides/química
Ésteres/química
Flores/química
Frutas/química
Physalis/química
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Cromatografia Líquida de Alta Pressão
Espectrometria de Massas
Physalis/classificação
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Esters); 0 (Plant Extracts); 36-88-4 (Carotenoids)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170811
[Lr] Data última revisão:
170811
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170712
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b02514


  3 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28617598
[Au] Autor:Xu YM; Wijeratne EMK; Babyak AL; Marks HR; Brooks AD; Tewary P; Xuan LJ; Wang WQ; Sayers TJ; Gunatilaka AAL
[Ad] Endereço:Natural Products Center, School of Natural Resources and the Environment, College of Agriculture and Life Sciences, University of Arizona , 250 E. Valencia Road, Tucson, Arizona 85706, United States.
[Ti] Título:Withanolides from Aeroponically Grown Physalis peruviana and Their Selective Cytotoxicity to Prostate Cancer and Renal Carcinoma Cells.
[So] Source:J Nat Prod;80(7):1981-1991, 2017 Jul 28.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Investigation of aeroponically grown Physalis peruviana resulted in the isolation of 11 new withanolides, including perulactones I-L (1-4), 17-deoxy-23ß-hydroxywithanolide E (5), 23ß-hydroxywithanolide E (6), 4-deoxyphyperunolide A (7), 7ß-hydroxywithanolide F (8), 7ß-hydroxy-17-epi-withanolide K (9), 24,25-dihydro-23ß,28-dihydroxywithanolide G (10), and 24,25-dihydrowithanolide E (11), together with 14 known withanolides (12-25). The structures of 1-11 were elucidated by the analysis of their spectroscopic data, and 12-25 were identified by comparison of their spectroscopic data with those reported. All withanolides were evaluated for their cytotoxic activity against a panel of tumor cell lines including LNCaP (androgen-sensitive human prostate adenocarcinoma), 22Rv1 (androgen-resistant human prostate adenocarcinoma), ACHN (human renal adenocarcinoma), M14 (human melanoma), SK-MEL-28 (human melanoma), and normal human foreskin fibroblast cells. Of these, the 17ß-hydroxywithanolides (17-BHWs) 6, 8, 9, 11-13, 15, and 19-22 showed selective cytotoxic activity against the two prostate cancer cell lines LNCaP and 22Rv1, whereas 13 and 20 exhibited selective toxicity for the ACHN renal carcinoma cell line. These cytotoxicity data provide additional structure-activity relationship information for the 17-BHWs.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/isolamento & purificação
Antineoplásicos Fitogênicos/farmacologia
Neoplasias Renais/tratamento farmacológico
Physalis/química
Neoplasias da Próstata/tratamento farmacológico
Vitanolídeos/isolamento & purificação
Vitanolídeos/farmacologia
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/química
Ensaios de Seleção de Medicamentos Antitumorais
Seres Humanos
Masculino
Melanoma/tratamento farmacológico
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Physalis/crescimento & desenvolvimento
Relação Estrutura-Atividade
Vitanolídeos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Withanolides)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170616
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b01129


  4 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28273367
[Au] Autor:Guo Y; Liu H; Ding L; Oppong M; Pan G; Qiu F
[Ad] Endereço:Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, 300193, China.
[Ti] Título:LC-MS/MS method for simultaneous determination of flavonoids and physalins in rat plasma: Application to pharmacokinetic study after oral administration of Physalis alkekengi var. franchetii (Chinese lantern) extract.
[So] Source:Biomed Chromatogr;31(10), 2017 Oct.
[Is] ISSN:1099-0801
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A rapid and sensitive liquid chromatography with tandem mass spectrometry (LC-MS/MS) method was developed and validated for the simultaneous determination of luteolin, luteolin-7-O-ß-D-glucopyranoside, physalin A, physalin D and physalin L in rat plasma. Scutellarein and dexamethasone were used as the internal standards (IS). Plasma samples were prepared by liquid-liquid extraction with ethyl acetate. The five constituents were separated on an Acquity UPLC BEH C column (100 mm × 2.1 mm, 1.7 µm). A gradient elution procedure was used with acetonitrile (A)-0.1% aqueous formic acid (B). Mass spectrometric detection was performed in negative ion multiple reaction monitoring mode with an electrospray ionization (ESI) source. This method showed good linearity (r > 0.997) over a concentration range of 2.0-500 ng/mL with a lower limit of quantification of 2.0 ng/mL for all five compounds. The inter- and intra-day accuracy ranged from 91.7 to 104%, and precisions (RSD) were <6.46% for all analytes. The extraction recoveries of all analytes were >85%. This validated method was successfully applied for the first time to the pharmacokinetic study of five ingredients after oral administration of 70% ethanol extract of Chinese lantern in rats.
[Mh] Termos MeSH primário: Cromatografia Líquida/métodos
Flavonoides/sangue
Physalis/química
Extratos Vegetais/administração & dosagem
Secoesteroides/sangue
Espectrometria de Massas em Tandem/métodos
[Mh] Termos MeSH secundário: Administração Oral
Animais
Estabilidade de Medicamentos
Flavonoides/química
Flavonoides/farmacocinética
Limite de Detecção
Modelos Lineares
Masculino
Extratos Vegetais/química
Extratos Vegetais/farmacocinética
Ratos
Ratos Sprague-Dawley
Reprodutibilidade dos Testes
Secoesteroides/química
Secoesteroides/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Flavonoids); 0 (Plant Extracts); 0 (Secosteroids)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170309
[St] Status:MEDLINE
[do] DOI:10.1002/bmc.3970


  5 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28152630
[Au] Autor:Yang YJ; Yi L; Wang Q; Xie BB; Dong Y; Sha CW
[Ad] Endereço:a Guangdong Food and Drug Vocational College , Guangdong Institute of Chinese Materia Medica , Guangzhou , China.
[Ti] Título:Anti-inflammatory effects of physalin E from Physalis angulata on lipopolysaccharide-stimulated RAW 264.7 cells through inhibition of NF-κB pathway.
[So] Source:Immunopharmacol Immunotoxicol;39(2):74-79, 2017 Apr.
[Is] ISSN:1532-2513
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Physalin E is a naturally occurring seco-steroid isolated from the stems and aerial parts of Physalis angulata L. (Solanaceae). This study was aimed to explore the anti-inflammatory effects of physalin E on RAW 264.7 mouse macrophages stimulated by lipopolysaccharide (LPS) and the potential underlying mechanisms. The results showed that physalin E significantly inhibited LPS-induced tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) expression and secretion in a dose-dependent manner. Unlike dexamethasone, these effects could not be blocked by miferstone (RU486). Meanwhile, physalin E reduced the degradation of I-kappa B protein in the cytoplasm and downregulated the nuclear factor-κB (NF-κB) p65 protein in the nuclear, which resulted in the inhibition of the NF-κB nuclear translocation. In conclusion, physalin E exerts its anti-inflammatory activities in LPS-induced macrophages. Physalin E can inhibit the production of inflammatory cytokines by targeting the NF-κB signaling pathway.
[Mh] Termos MeSH primário: Lipopolissacarídeos/toxicidade
NF-kappa B/imunologia
Physalis/química
Secoesteroides/farmacologia
Transdução de Sinais/efeitos dos fármacos
[Mh] Termos MeSH secundário: Animais
Interleucina-6/imunologia
Camundongos
Células RAW 264.7
Secoesteroides/química
Transdução de Sinais/imunologia
Fator de Necrose Tumoral alfa/imunologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Interleukin-6); 0 (Lipopolysaccharides); 0 (NF-kappa B); 0 (Secosteroids); 0 (Tumor Necrosis Factor-alpha); 0 (interleukin-6, mouse); 0 (physalin E)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170204
[St] Status:MEDLINE
[do] DOI:10.1080/08923973.2017.1282514


  6 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28059765
[Au] Autor:Wilf P; Carvalho MR; Gandolfo MA; Cúneo NR
[Ad] Endereço:Department of Geosciences, Pennsylvania State University, University Park, PA 16802, USA. pwilf@psu.edu.
[Ti] Título:Eocene lantern fruits from Gondwanan Patagonia and the early origins of Solanaceae.
[So] Source:Science;355(6320):71-75, 2017 01 06.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The nightshade family Solanaceae holds exceptional economic and cultural importance. The early diversification of Solanaceae is thought to have occurred in South America during its separation from Gondwana, but the family's sparse fossil record provides few insights. We report 52.2-million-year-old lantern fruits from terminal-Gondwanan Patagonia, featuring highly inflated, five-lobed calyces, as a newly identified species of the derived, diverse New World genus Physalis (e.g., groundcherries and tomatillos). The fossils are considerably older than corresponding molecular divergence dates and demonstrate an ancient history for the inflated calyx syndrome. The derived position of these early Eocene fossils shows that Solanaceae were well diversified long before final Gondwanan breakup.
[Mh] Termos MeSH primário: Frutas/classificação
Physalis/classificação
[Mh] Termos MeSH secundário: Argentina
Chile
Fósseis
Frutas/anatomia & histologia
Filogenia
Filogeografia
Physalis/anatomia & histologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170802
[Lr] Data última revisão:
170802
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE
[do] DOI:10.1126/science.aag2737


  7 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28033720
[Au] Autor:Sun CP; Nie XF; Kang N; Zhao F; Chen LX; Qiu F
[Ad] Endereço:a Tianjin State Key Laboratory of Modern Chinese Medicine and School of Chinese Materia Medica , Tianjin University of Traditional Chinese Medicine , Tianjin , China.
[Ti] Título:A new phenol glycoside from Physalis angulata.
[So] Source:Nat Prod Res;31(9):1059-1065, 2017 May.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new phenol glycoside, physanguloside A (1), was isolated from Physalis angulata together with four known compounds. We report herein, for the first time, the presence of compounds 2-5 in the genus Physalis. The structures of all the compounds were established by NMR, IR, UV and HRESIMS spectroscopic analyses, and comparison with the literature data. All isolated compounds were assayed for inhibitory activity on nitric oxide production by LPS-induced in RAW 264.7 macrophages.
[Mh] Termos MeSH primário: Glicosídeos/isolamento & purificação
Physalis/química
[Mh] Termos MeSH secundário: Animais
Glicosídeos/química
Glicosídeos/farmacologia
Macrófagos/efeitos dos fármacos
Camundongos
Óxido Nítrico/antagonistas & inibidores
Óxido Nítrico/biossíntese
Fenóis/química
Fenóis/isolamento & purificação
Fenóis/farmacologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Glycosides); 0 (Phenols); 0 (physanguloside A); 31C4KY9ESH (Nitric Oxide)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161231
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2016.1269102


  8 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27932519
[Au] Autor:Pinheiro PV; Ghanim M; Alexander M; Rebelo AR; Santos RS; Orsburn BC; Gray S; Cilia M
[Ad] Endereço:From the ‡Department of Entomology, Cornell University, Ithaca, New York 14853.
[Ti] Título:Host Plants Indirectly Influence Plant Virus Transmission by Altering Gut Cysteine Protease Activity of Aphid Vectors.
[So] Source:Mol Cell Proteomics;16(4 suppl 1):S230-S243, 2017 Apr.
[Is] ISSN:1535-9484
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The green peach aphid, , is a vector of the (PLRV, Luteoviridae), transmitted exclusively by aphids in a circulative manner. PLRV transmission efficiency was significantly reduced when a clonal lineage of was reared on turnip as compared with the weed physalis, and this was a transient effect caused by a host-switch response. A trend of higher PLRV titer in physalis-reared aphids as compared with turnip-reared aphids was observed at 24 h and 72 h after virus acquisition. The major difference in the proteomes of these aphids was the up-regulation of predicted lysosomal enzymes, in particular the cysteine protease cathepsin B (cathB), in aphids reared on turnip. The aphid midgut is the site of PLRV acquisition, and cathB and PLRV localization were starkly different in midguts of the aphids reared on the two host plants. In viruliferous aphids that were reared on turnip, there was near complete colocalization of cathB and PLRV at the cell membranes, which was not observed in physalis-reared aphids. Chemical inhibition of cathB restored the ability of aphids reared on turnip to transmit PLRV in a dose-dependent manner, showing that the increased activity of cathB and other cysteine proteases at the cell membrane indirectly decreased virus transmission by aphids. Understanding how the host plant influences virus transmission by aphids is critical for growers to manage the spread of virus among field crops.
[Mh] Termos MeSH primário: Afídeos/virologia
Brassica napus/parasitologia
Catepsina B/metabolismo
Luteoviridae/fisiologia
Physalis/parasitologia
[Mh] Termos MeSH secundário: Animais
Afídeos/enzimologia
Afídeos/fisiologia
Trato Gastrointestinal/enzimologia
Trato Gastrointestinal/virologia
Interações Hospedeiro-Parasita
Proteínas de Insetos/metabolismo
Insetos Vetores/enzimologia
Insetos Vetores/fisiologia
Insetos Vetores/virologia
Doenças das Plantas/virologia
Vírus de Plantas/fisiologia
Proteômica/métodos
Regulação para Cima
Carga Viral
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Insect Proteins); EC 3.4.22.1 (Cathepsin B)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161210
[St] Status:MEDLINE
[do] DOI:10.1074/mcp.M116.063495


  9 / 212 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:27913954
[Au] Autor:Li RJ; Gao CY; Guo C; Zhou MM; Luo J; Kong LY
[Ad] Endereço:State Key Laboratory of Natural Medicines, Department of Natural Medicinal Chemistry, China Pharmaceutical University, 24 Tong Jia Xiang, Nanjing, 210009, People's Republic of China.
[Ti] Título:The Anti-inflammatory Activities of Two Major Withanolides from Physalis minima Via Acting on NF-κB, STAT3, and HO-1 in LPS-Stimulated RAW264.7 Cells.
[So] Source:Inflammation;40(2):401-413, 2017 Apr.
[Is] ISSN:1573-2576
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Physalis minima has been traditionally used as a folk herbal medicine in China for the treatment of many inflammatory diseases. However, little is known about its anti-inflammatory constituents and associated molecular mechanisms. In our study, withaphysalin A (WA) and 2, 3-dihydro-withaphysalin C (WC), two major withanolide-type compounds, were obtained from the anti-inflammatory fraction of P. minima. Both WA and WC significantly inhibited the production of nitrite oxide (NO), prostaglandin E (PGE ), and several pro-inflammatory cytokines, such as interleukin-1ß (IL-1ß), IL-6, and tumor necrosis factor-α (TNF-α) in lipopolysaccharide (LPS)-activated RAW264.7 macrophages. Further research indicated that they downregulated the LPS-induced expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) at the mRNA and protein levels. In addition, they also suppressed nuclear translocation of NF-κB p65, phosphorylation of STAT3, and upregulated HO-1 expression. Intriguingly, the activation of MAPKs was suppressed by WA but was not altered by WC. Taken together, these data provide scientific evidence for elucidating the major bioactive constituents and related molecular mechanisms for the traditional use of P. minima and suggest that WA and WC can be attractive therapeutic candidates for various inflammatory diseases.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Ergosterol/análogos & derivados
Physalis/química
Secoesteroides/farmacologia
Vitanolídeos/farmacologia
[Mh] Termos MeSH secundário: Animais
Ciclo-Oxigenase 2/efeitos dos fármacos
Ciclo-Oxigenase 2/metabolismo
Ergosterol/isolamento & purificação
Ergosterol/farmacologia
Heme Oxigenase-1/metabolismo
Mediadores da Inflamação/metabolismo
Lipopolissacarídeos
Proteínas de Membrana/metabolismo
Camundongos
NF-kappa B/metabolismo
Óxido Nítrico Sintase Tipo II/efeitos dos fármacos
Óxido Nítrico Sintase Tipo II/metabolismo
Células RAW 264.7
Fator de Transcrição STAT3/metabolismo
Secoesteroides/isolamento & purificação
Vitanolídeos/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Inflammation Mediators); 0 (Lipopolysaccharides); 0 (Membrane Proteins); 0 (NF-kappa B); 0 (STAT3 Transcription Factor); 0 (Secosteroids); 0 (Withanolides); 0 (withaphysalin A); 57485-60-6 (withaphysalin C); EC 1.14.13.39 (Nitric Oxide Synthase Type II); EC 1.14.14.18 (Heme Oxygenase-1); EC 1.14.14.18 (Hmox1 protein, mouse); EC 1.14.99.1 (Cyclooxygenase 2); Z30RAY509F (Ergosterol)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:171020
[Lr] Data última revisão:
171020
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161204
[St] Status:MEDLINE
[do] DOI:10.1007/s10753-016-0485-1


  10 / 212 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
[PMID]:27440155
[Au] Autor:Balaguera-López HE; Espinal-Ruiz M; Zacarías L; Herrera AO
[Ad] Endereço:Departamento de Agronomía, Universidad Pedagógica y Tecnológica de Colombia, Tunja, Colombia.
[Ti] Título:Effect of ethylene and 1-methylcyclopropene on the postharvest behavior of cape gooseberry fruits (Physalis peruviana L.).
[So] Source:Food Sci Technol Int;23(1):86-96, 2017 Jan.
[Is] ISSN:1532-1738
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cape gooseberry (Physalis peruviana L.) fruits are highly perishable berries that exhibit a climacteric respiratory behavior. The objective of this study was to evaluate the effect of ethylene and the ethylene action inhibitor 1-methylcyclopropene on the postharvest behavior of cape gooseberry fruits (ecotype Colombia). Fruits were treated with ethylene, in an ethephon application (1000 µL L ), and pretreated with 1-methylcyclopropene (1 µL L ), 1-methylcyclopropene+ethylene, and results compared with a control without application. Subsequently, the fruits were maintained at room temperature (20 ℃, 75% RH) for up to 11 days. The pretreatment of the cape gooseberry fruits with 1-methylcyclopropene delayed most of the ripening-associated parameters, with a reduction in the respiration rate and ethylene production, skin color development, total soluble solids, total carotenoid content, loss of firmness, loss of total titratable acidity and emission of volatile compounds such as ethyl octanoate, ethyl butanoate, ethyl decanoate, and hexyl decanoate. Conversely, application of ethephon accelerated most of these physiological changes and also overcame most of the effects prevented by the ethylene action inhibitor. Altogether, the results supported the idea of a climacteric-like behavior for cape gooseberry fruits and pointing out that the pretreatment with 1-methylcyclopropene may be a promising and efficient postharvest treatment to delay maturity and extend the postharvest period.
[Mh] Termos MeSH primário: Conservantes de Alimentos/química
Frutas/química
Physalis/química
[Mh] Termos MeSH secundário: Carotenoides/análise
Cor
Comportamento do Consumidor
Ciclopropanos/química
Etilenos/química
Análise de Alimentos
Armazenamento de Alimentos
Valor Nutritivo
Paladar
Temperatura Ambiente
Compostos Orgânicos Voláteis/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclopropanes); 0 (Ethylenes); 0 (Food Preservatives); 0 (Volatile Organic Compounds); 36-88-4 (Carotenoids); 91GW059KN7 (ethylene); J6UJO23JGU (1-methylcyclopropene)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170410
[Lr] Data última revisão:
170410
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160722
[St] Status:MEDLINE



página 1 de 22 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde