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Pesquisa : B01.650.940.800.575.912.250.942.500 [Categoria DeCS]
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[PMID]:28375765
[Au] Autor:Traesel GK; Machado CD; Tirloni CAS; Menetrier JV; Dos Reis Lívero FA; Lourenço ELB; Oesterreich SA; Budel JM; Junior AG
[Ad] Endereço:1 Faculty of Health Sciences, Federal University of Grande Dourados , Dourados, Brazil .
[Ti] Título:Safety Assessment and Botanical Standardization of an Edible Species from South America.
[So] Source:J Med Food;20(5):519-525, 2017 May.
[Is] ISSN:1557-7600
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Tropaeolum majus L. (Tropaeolaceae), commonly known as nasturtium, is an important edible plant native to the Andean States and widely disseminated throughout South America. Despite the use of this species is quite widespread, there are no minimum quality control standards or data on its genotoxicity. So, the aim of this study was to present a detailed anatomical and histochemical study for Tropaeolum majus and provide genotoxicity parameters of a preparation routinely used in South American countries. First, three different Tropaeolum majus aqueous extracts (TMAEs) at concentrations of 1.5%, 7%, and 15% were prepared according to the popular use. Then, genetic toxicity of TMAE was evaluated on bacterial reverse mutation, genomic lesions, and micronucleus formation in male rats. Furthermore, a detailed anatomical and histochemical study of the leaves and stems of Tropaeolum majus were performed. No revertant colonies were found in any bacterial cultures examined. In the comet assay, TMAE showed no significant DNA damage in all tested doses. Micronucleus assay showed no significant increases in the frequency of inducing micronuclei in any dose examined. Light and electron microscope images of cross-section of leaves and stems from Tropaeolum majus revealed useful diagnostic features. The presented data showed significant safety parameters for the use of TMAE and provided important data for the quality control of this plant species.
[Mh] Termos MeSH primário: Extratos Vegetais/química
Tropaeolum/química
[Mh] Termos MeSH secundário: Animais
Bactérias/efeitos dos fármacos
Bactérias/genética
Masculino
Testes para Micronúcleos
Testes de Mutagenicidade
Extratos Vegetais/toxicidade
Folhas de Planta/química
Folhas de Planta/toxicidade
Ratos
América do Sul
Tropaeolum/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Extracts)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170822
[Lr] Data última revisão:
170822
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170405
[St] Status:MEDLINE
[do] DOI:10.1089/jmf.2016.0143


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[PMID]:27482986
[Au] Autor:Botelho Lourenço EL; Lima Ribeiro RC; Araújo VO; Martino-Andrade AJ; Dalsenter PR; Gasparotto A
[Ad] Endereço:a Laboratory of Pharmacology and Toxicology of Natural Products , Universidade Paranaense , Umuarama , PR , Brazil.
[Ti] Título:Fetopathies associated with exposure to angiotensin converting enzyme inhibitor from Tropaeolum majus L.
[So] Source:Drug Chem Toxicol;40(3):281-285, 2017 Jul.
[Is] ISSN:1525-6014
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The prevalence of the use of herbal medicines is on the rise across the world, especially amongst pregnant women. A fact that draws attention is that many species commonly used by pregnant women, including the Tropaeolum majus L. (Tropaeolaceae), also present inhibitory activity on the angiotensin-converting enzyme (ACE). Herein, we have investigated the effects of T. majus extract (HETM) on fetal development, evaluating its relationship with possible ACE inhibitory activity. Pregnant Wistar rats were treated with different HETM doses (3, 30 and 300 mg/kg/day) from gestational days 8-20. Rats were sacrificed on the day 20 of pregnancy and the following parameters were evaluated: clinical symptoms of maternal toxicity; maternal body weight; feed and water intake; maternal liver, kidney, and ovary weights, maternal ACE activity and aldosterone levels, live fetuses mean; dead fetuses percentage, fetus weight, and fetal malformation. All pregnant rats treated with high HETM doses showed significant reduction in plasma ACE activity accompanied by a decrease in serum aldosterone levels. Moreover, significant changes in fetal development were observed, including growth retardation and renal damage after 20 days of gestation. Thus, data presented demonstrate the significant effects of the use of HETM on fetal development during pregnancy.
[Mh] Termos MeSH primário: Anormalidades Induzidas por Medicamentos/etiologia
Inibidores da Enzima Conversora de Angiotensina/toxicidade
Desenvolvimento Fetal/efeitos dos fármacos
Retardo do Crescimento Fetal/induzido quimicamente
Extratos Vegetais/toxicidade
Tropaeolum/química
[Mh] Termos MeSH secundário: Inibidores da Enzima Conversora de Angiotensina/isolamento & purificação
Animais
Relação Dose-Resposta a Droga
Feminino
Idade Gestacional
Exposição Materna/efeitos adversos
Extratos Vegetais/isolamento & purificação
Gravidez
Ratos Wistar
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Angiotensin-Converting Enzyme Inhibitors); 0 (Plant Extracts)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170906
[Lr] Data última revisão:
170906
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160803
[St] Status:MEDLINE
[do] DOI:10.1080/01480545.2016.1212366


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[PMID]:27622707
[Au] Autor:Guzmán-Pérez V; Bumke-Vogt C; Schreiner M; Mewis I; Borchert A; Pfeiffer AF
[Ad] Endereço:Department of Clinical Nutrition, German Institute of Human Nutrition, Potsdam-Rehbrücke, Nuthetal, Germany.
[Ti] Título:Benzylglucosinolate Derived Isothiocyanate from Tropaeolum majus Reduces Gluconeogenic Gene and Protein Expression in Human Cells.
[So] Source:PLoS One;11(9):e0162397, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Nasturtium (Tropaeolum majus L.) contains high concentrations of benzylglcosinolate. We found that a hydrolysis product of benzyl glucosinolate-the benzyl isothiocyanate (BITC)-modulates the intracellular localization of the transcription factor Forkhead box O 1 (FOXO1). FoxO transcription factors can antagonize insulin effects and trigger a variety of cellular processes involved in tumor suppression, longevity, development and metabolism. The current study evaluated the ability of BITC-extracted as intact glucosinolate from nasturtium and hydrolyzed with myrosinase-to modulate i) the insulin-signaling pathway, ii) the intracellular localization of FOXO1 and, iii) the expression of proteins involved in gluconeogenesis, antioxidant response and detoxification. Stably transfected human osteosarcoma cells (U-2 OS) with constitutive expression of FOXO1 protein labeled with GFP (green fluorescent protein) were used to evaluate the effect of BITC on FOXO1. Human hepatoma HepG2 cell cultures were selected to evaluate the effect on gluconeogenic, antioxidant and detoxification genes and protein expression. BITC reduced the phosphorylation of protein kinase B (AKT/PKB) and FOXO1; promoted FOXO1 translocation from cytoplasm into the nucleus antagonizing the insulin effect; was able to down-regulate the gene and protein expression of gluconeogenic enzymes; and induced the gene expression of antioxidant and detoxification enzymes. Knockdown analyses with specific siRNAs showed that the expression of gluconeogenic genes was dependent on nuclear factor (erythroid derived)-like2 (NRF2) and independent of FOXO1, AKT and NAD-dependent deacetylase sirtuin-1 (SIRT1). The current study provides evidence that BITC might have a role in type 2 diabetes T2D by reducing hepatic glucose production and increasing antioxidant resistance.
[Mh] Termos MeSH primário: Gluconeogênese/efeitos dos fármacos
Gluconeogênese/genética
Tiocianatos/farmacologia
Tioglucosídeos/farmacologia
Tropaeolum/química
[Mh] Termos MeSH secundário: Acetilcisteína/farmacologia
Transporte Ativo do Núcleo Celular/efeitos dos fármacos
Antioxidantes/farmacologia
Linhagem Celular
Sobrevivência Celular/efeitos dos fármacos
Diabetes Mellitus Tipo 2/tratamento farmacológico
Diabetes Mellitus Tipo 2/genética
Diabetes Mellitus Tipo 2/metabolismo
Proteína Forkhead Box O1/antagonistas & inibidores
Proteína Forkhead Box O1/genética
Proteína Forkhead Box O1/metabolismo
Expressão Gênica/efeitos dos fármacos
Técnicas de Silenciamento de Genes
Gluconeogênese/fisiologia
Glucose-6-Fosfatase/genética
Células Hep G2
Seres Humanos
Hipoglicemiantes/química
Hipoglicemiantes/farmacologia
Isotiocianatos/química
Isotiocianatos/farmacologia
Fator 2 Relacionado a NF-E2/antagonistas & inibidores
Fator 2 Relacionado a NF-E2/genética
Fosfoenolpiruvato Carboxiquinase (ATP)/genética
Plantas Medicinais/química
Transporte Proteico/efeitos dos fármacos
Proteínas Proto-Oncogênicas c-akt/antagonistas & inibidores
Proteínas Proto-Oncogênicas c-akt/genética
RNA Interferente Pequeno/genética
Proteínas Recombinantes de Fusão/genética
Proteínas Recombinantes de Fusão/metabolismo
Sirtuína 1/antagonistas & inibidores
Sirtuína 1/genética
Tiocianatos/química
Tioglucosídeos/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (FOXO1 protein, human); 0 (Forkhead Box Protein O1); 0 (Hypoglycemic Agents); 0 (Isothiocyanates); 0 (NF-E2-Related Factor 2); 0 (NFE2L2 protein, human); 0 (RNA, Small Interfering); 0 (Recombinant Fusion Proteins); 0 (Thiocyanates); 0 (Thioglucosides); 0 (benzylglucosinolic acid); 871J6YOR8Q (benzyl isothiocyanate); EC 2.7.11.1 (Proto-Oncogene Proteins c-akt); EC 3.1.3.9 (Glucose-6-Phosphatase); EC 3.5.1.- (SIRT1 protein, human); EC 3.5.1.- (Sirtuin 1); EC 4.1.1.49 (PCK2 protein, human); EC 4.1.1.49 (Phosphoenolpyruvate Carboxykinase (ATP)); WYQ7N0BPYC (Acetylcysteine)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170808
[Lr] Data última revisão:
170808
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160914
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0162397


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[PMID]:27161402
[Au] Autor:Tran HT; Márton MR; Herz C; Maul R; Baldermann S; Schreiner M; Lamy E
[Ad] Endereço:University of Freiburg - Medical Center, Institute of Environmental Health Sciences, Molecular Preventive Medicine, Breisacher Strasse 115b, 79106 Freiburg, Germany; University of Freiburg - Medical Center, Institute for Prevention and Cancer Epidemiology, Molecular Preventive Medicine, Elsässerstr.
[Ti] Título:Nasturtium (Indian cress, Tropaeolum majus nanum) dually blocks the COX and LOX pathway in primary human immune cells.
[So] Source:Phytomedicine;23(6):611-20, 2016 Jun 01.
[Is] ISSN:1618-095X
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Nasturtium (Indian cress, Tropaeolum majus) is known for its pharmacological value in the treatment of bacterial infections of the upper air tract and urinary bladder. However, scientific data on the anti-inflammatory potency in human-derived cells is missing. PURPOSE: The aim of this study was to investigate the potential of nasturtium to inhibit the lipopolysaccharide (LPS) induced inflammatory response in primary human cells of the immune system. STUDY DESIGN: The anti-inflammatory activities of nasturtium and its fractions were evaluated via regulation of arachidonic acid (AA) pathway and MAPK kinase cascade. Fraction H4 which was responsible for the anti-inflammatory effects was further characterized. METHODS: Human peripheral blood mononuclear cells (PBMC) were either treated with plant extracts or fractions thereof, stimulated with LPS and/or N-formyl-methionyl-leucyl-phenylalanine (fMLP) and analysed for COX and LOX, release of prostaglandin PGE2, leukotriene LTB4, TNF-alpha and ERK signaling pathway activation. The plant extracts were separated into four fractions by HPLC; fraction H4 was subjected to UHPLC-ToF/MS analysis to identify potential bioactive compounds. RESULTS: We found that aqueous extracts of nasturtium did exert strong concentration dependent suppression of LPS-triggered TNF-alpha release and COX pathway signaling, including PGE2 synthesis. Whereas COX-1 protein expression was not impacted, LPS-triggered COX-2 protein expression was concentration dependently blocked by the plant extract but not COX-2 enzyme activity. These findings suggest a mechanism of action for the plant extract which is different from non-steroidal anti-inflammatory drugs (NSAIDs). Moreover, the plant extract blocked leukotriene LTB4 release, the major end product of the 5-LOX pathway from PBMC. Down-regulation of ERK1/2 and c-Jun activation preceded COX-2 suppression upon plant extract treatment in the presence of LPS. Using HPLC separation of the aqueous extract followed by metabolomic analysis we could limit the number of relevant bioactive compounds in the extract to about 50. CONCLUSIONS: This study provides a rationale for the anti-inflammatory efficacy of nasturtium observed in man and gives first insight into the underlying molecular mechanisms.
[Mh] Termos MeSH primário: Anti-Inflamatórios/farmacologia
Ciclo-Oxigenase 2/metabolismo
Inibidores Enzimáticos/farmacologia
Inflamação/induzido quimicamente
Inflamação/tratamento farmacológico
Lipopolissacarídeos/efeitos adversos
Lipoxigenase/metabolismo
[Mh] Termos MeSH secundário: Animais
Seres Humanos
Masculino
Nasturtium/química
Extratos Vegetais/farmacologia
Tropaeolum/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Inflammatory Agents); 0 (Enzyme Inhibitors); 0 (Lipopolysaccharides); 0 (Plant Extracts); EC 1.13.11.12 (Lipoxygenase); EC 1.14.99.1 (Cyclooxygenase 2)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170817
[Lr] Data última revisão:
170817
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160511
[St] Status:MEDLINE


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ALVES, Sydney Hartz
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[PMID]:27136515
[Au] Autor:da Cruz RC; Denardi LB; Mossmann NJ; Piana M; Alves SH; de Campos MM
[Ad] Endereço:Departamento de Farmácia Industrial, Universidade Federal de Santa Maria (UFSM), Avenida Roraima 1000, Block 26, Santa Maria 97105-900, Rio Grande do Sul, Brazil. ritieldc@gmail.com.
[Ti] Título:Antimicrobial Activity and Chromatographic Analysis of Extracts from Tropaeolum pentaphyllum Lam. Tubers.
[So] Source:Molecules;21(5), 2016 Apr 28.
[Is] ISSN:1420-3049
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Tropaeolum pentaphyllum Lam. tubers (Tropaeolaceae) are known and used as a condiment and for the treatment of skin infections in Southern Brazil. However, its activity and composition has not yet been investigated. Thus, different extracts and the essential oil from the tubers were tested against a range of microorganisms. The most active extracts were submitted to chromatographic analysis. METHODS: Hydroalcoholic extract (70%), fractions of it, and the essential oil from the tubers were tested against several bacteria, yeasts and molds, furnishing the corresponding inhibitory, bactericidal and fungicidal minimal concentration values. The most active extracts were submitted to GC-MS investigation. RESULTS: The strongest effects against different strains of microorganisms, such as Gram-positive and negative bacteria, Candida spp. and dermatophytes were observed for the essential oil and the chloroform fraction, with minimal inhibitory concentrations (MICs) well below 200 µg/mL. GC-MS analysis revealed that the major essential oil constituent is benzyl isothiocyanate (BITC), while the chloroform fraction is constituted of BITC, amides, sulfur, fatty acids and its esters, all compounds that may be related to the demonstrated activity. CONCLUSIONS: Overall, the results support the popular use of the plant for the treatment of skin infections, and revealed the main active compounds.
[Mh] Termos MeSH primário: Extratos Vegetais/isolamento & purificação
Extratos Vegetais/farmacologia
Tubérculos/química
Tropaeolum/química
[Mh] Termos MeSH secundário: Antibacterianos/química
Antibacterianos/farmacologia
Antifúngicos/química
Antifúngicos/farmacologia
Bactérias/efeitos dos fármacos
Cromatografia Gasosa-Espectrometria de Massas
Testes de Sensibilidade Microbiana
Óleos Voláteis/química
Óleos Voláteis/farmacologia
Extratos Vegetais/química
Leveduras/efeitos dos fármacos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Bacterial Agents); 0 (Antifungal Agents); 0 (Oils, Volatile); 0 (Plant Extracts)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170306
[Lr] Data última revisão:
170306
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160503
[St] Status:MEDLINE


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[PMID]:26919585
[Au] Autor:Martín JC; Higuera BL
[Ad] Endereço:Department of Chemistry, Sciences Faculty, Universidad Nacional de Colombia, Bogotá, Colombia. jennycmartinr@gmail.com.
[Ti] Título:Glucosinolate composition of Colombian accessions of mashua (Tropaeolum tuberosum Ruíz & Pavón), structural elucidation of the predominant glucosinolate and assessment of its antifungal activity.
[So] Source:J Sci Food Agric;96(14):4702-4712, 2016 Nov.
[Is] ISSN:1097-0010
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The content of individual and total glucosinolates in 65 mashua tuber accessions (Tropaeolum tuberosum) from the germplasm bank at Universidad Nacional de Colombia was determined by reverse phase high-performance liquid chromatography on enzymatically desulfated extracts. The predominant glucosinolate was identified and the possible structure of the glucosinolate present in lower proportion was postulated from evidence obtained by high-performance liquid chromatography/mass spectrometry, H and C nuclear magnetic resonance and bi-dimensional experiments. The biological action of the hydrolysis products generated from the glucosinolates in the accessions that showed a higher content of these compounds was assessed in the presence of Fusarium oxysporum f. sp. dianthi, Rhizoctonia solani and Phytophthora infestans. RESULTS: The total content of glucosinolates ranged between >3.00 × 10 and 25.8 µmol g dry matter. p-Methoxybenzyl glucosinolate was identified as the predominant glucosinolate in Colombian mashua accessions; besides, the possible presence of p-hydroxybenzyl glucosinolate was postulated. In vitro assays established an important fungal growth inhibition of the potato pathogen P. infestans. CONCLUSION: The biological action from p-methoxybenzyl glucosinolate and p-hydroxybenzyl glucosinolate found in Colombian mashua accessions depends on their concentration, with the Tt30 accession, characterized for showing the highest content of glucosinolates, being the most promising to control the assessed pathogens. © 2016 Society of Chemical Industry.
[Mh] Termos MeSH primário: Antifúngicos/farmacologia
Fungos/efeitos dos fármacos
Glucosinolatos/química
Glucosinolatos/farmacologia
Tropaeolum/química
[Mh] Termos MeSH secundário: Antifúngicos/química
Configuração de Carboidratos
Colômbia
Doenças das Plantas/microbiologia
Solanum tuberosum/microbiologia
Tropaeolum/genética
Tropaeolum/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antifungal Agents); 0 (Glucosinolates)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170519
[Lr] Data última revisão:
170519
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160227
[St] Status:MEDLINE
[do] DOI:10.1002/jsfa.7689


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[PMID]:26610401
[Au] Autor:Platz S; Kühn C; Schiess S; Schreiner M; Kemper M; Pivovarova O; Pfeiffer AF; Rohn S
[Ad] Endereço:Hamburg School of Food Science, Institute of Food Chemistry, University of Hamburg, Hamburg, Germany.
[Ti] Título:Bioavailability and metabolism of benzyl glucosinolate in humans consuming Indian cress (Tropaeolum majus L.).
[So] Source:Mol Nutr Food Res;60(3):652-60, 2016 Mar.
[Is] ISSN:1613-4133
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:SCOPE: Benzyl isothiocyanate (BITC), which occurs in Brassicales, has demonstrated chemopreventive potency and cancer treatment properties in cell and animal studies. However, fate of BITC in human body is not comprehensively studied. Therefore, the present human intervention study investigates the metabolism of the glucosinolate (GSL) glucotropaeolin and its corresponding BITC metabolites. Analyzing BITC metabolites in plasma and urine should reveal insights about resorption, metabolism, and excretion. METHODS AND RESULTS: Fifteen healthy men were randomly recruited for a cross-over study and consumed 10 g freeze-dried Indian cress as a liquid preparation containing 1000 µmol glucotropaeolin. Blood and urine samples were taken at several time points and investigated by LC-ESI-MS/MS after sample preparation using SPE. Plasma contained high levels of BITC-glutathione (BITC-GSH), BITC-cysteinylglycine (BITC-CysGly), and BITC-N-acetyl-L-cysteine (BITC-NAC) 1-5 h after ingestion, with BITC-CysGly appearing as the main metabolite. Compared to human plasma, the main urinary metabolites were BITC-NAC and BITC-Cys, determined 4-6 h after ingestion. CONCLUSION: This study confirms that consumption of Indian cress increases the concentration of BITC metabolites in human plasma and urine. The outcome of this human intervention study supports clinical research dealing with GSL-containing innovative food products or pharmaceutical preparations.
[Mh] Termos MeSH primário: Tiocianatos/farmacocinética
Tioglucosídeos/farmacocinética
Tropaeolum
[Mh] Termos MeSH secundário: Disponibilidade Biológica
Estudos Cross-Over
Seres Humanos
Isotiocianatos/farmacocinética
Masculino
Experimentação Humana não Terapêutica
Distribuição Aleatória
Espectrometria de Massas em Tandem
Tiocianatos/metabolismo
Tioglucosídeos/metabolismo
Tropaeolum/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Isothiocyanates); 0 (Thiocyanates); 0 (Thioglucosides); 0 (benzylglucosinolic acid); 499-26-3 (glucotropeolin)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151128
[St] Status:MEDLINE
[do] DOI:10.1002/mnfr.201500633


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[PMID]:26068019
[Au] Autor:Zacharski DM; Brandt S; Esch S; König S; Mormann M; Ulrich-Merzenich G; Hensel A
[Ad] Endereço:‡University of Münster, Institute of Pharmaceutical Biology and Phytochemistry, Corrensstrasse 48, D-48149 Münster, Germany.
[Ti] Título:Xyloglucan from Tropaeolum majus Seeds Induces Cellular Differentiation of Human Keratinocytes by Inhibition of EGFR Phosphorylation and Decreased Activity of Transcription Factor CREB.
[So] Source:Biomacromolecules;16(7):2157-67, 2015 Jul 13.
[Is] ISSN:1526-4602
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Xyloglucan XG (molecular weight 462 kDa, 1,4-/1,4,6-(pGlc) linked backbone, side chains of 1-pXyl, 1,2-pXyl, 1-p-Gal) was isolated from the seeds of Tropaeolum majus. XG (100 µg/mL) induced terminal cellular differentiation of human keratinocytes, as demonstrated by immunofluorescence staining and Western blot using cytokeratin 10 and involucrin as marker proteins. Differentiation was also induced by XG-derived oligosaccharides (degree of polymerization 7-9). Quantitative real-time polymerase chain reaction (qPCR) revealed the induction of gene expression of typical differentiation markers (cytokeratin, filaggrin, involucrin, loricrin, transglutaminase) in a time-dependent manner. Whole human genome microarray indicated that most of upregulated genes were related to differentiation processes. Microarray findings on selected genes were subsequently confirmed by qPCR. For identification of the molecular target of xyloglucan PAGE of keratinocyte membrane preparations was performed, followed by blotting with fluorescein isothiocyanate-labeled XG. XG interacting proteins were characterized by MS. Peptide fragments of epidermal growth factor receptor (EGFR) and integrin ß4 were identified. Subsequent phospho-kinase array indicated that phosphorylation of EGFR and transcription factor cAMP response element-binding protein (CREB) was decreased in the XG-treated cells. Thus, the XG-induced differentiation of keratinocytes is proposed to be mediated by the inhibition of the phosphorylation of EGFR, leading to a dimished CREB activation, which is essential for the activation of cellular differentiation.
[Mh] Termos MeSH primário: Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico/metabolismo
Glucanos/farmacologia
Queratinócitos/efeitos dos fármacos
Receptor do Fator de Crescimento Epidérmico/metabolismo
Tropaeolum/química
Xilanos/farmacologia
[Mh] Termos MeSH secundário: Diferenciação Celular
Células Cultivadas
Regulação da Expressão Gênica/efeitos dos fármacos
Seres Humanos
Queratinócitos/citologia
Queratinócitos/metabolismo
Fosforilação/efeitos dos fármacos
Sementes/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (CREB1 protein, human); 0 (Cyclic AMP Response Element-Binding Protein); 0 (Glucans); 0 (Xylans); 37294-28-3 (xyloglucan); EC 2.7.10.1 (EGFR protein, human); EC 2.7.10.1 (Receptor, Epidermal Growth Factor)
[Em] Mês de entrada:1604
[Cu] Atualização por classe:150713
[Lr] Data última revisão:
150713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150613
[St] Status:MEDLINE
[do] DOI:10.1021/acs.biomac.5b00553


  9 / 41 MEDLINE  
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[PMID]:25659835
[Au] Autor:Garzón GA; Manns DC; Riedl K; Schwartz SJ; Padilla-Zakour O
[Ad] Endereço:Departamento de Química, Universidad Nacional de Colombia , AA 14490 Bogotá, Colombia.
[Ti] Título:Identification of phenolic compounds in petals of nasturtium flowers (Tropaeolum majus) by high-performance liquid chromatography coupled to mass spectrometry and determination of oxygen radical absorbance capacity (ORAC).
[So] Source:J Agric Food Chem;63(6):1803-11, 2015 Feb 18.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The contents and profile of polyphenols were analyzed in edible petals of nasturtium flowers (Tropaeolum majus) of three colors, and their oxygen radical absorbance capacities (ORAC) were compared. Three primary anthocyanins (ACNs) and 15 non-ACN phenolic compounds including hydroxycinammic acids (HCAs) and flavonoids (myricetin, quercetin, and kaempferol derivatives) were detected. Anthocyanin concentration was within 31.9 ± 21.7 and 114.5 ± 2.3 mg cyanidin-3-glucoside (cy-3-glu)/100 g fresh weight (FW) in yellow and red petals, respectively. The concentration of HCAs varied between 33.3 ± 7.1 and 235.6 ± 8.1 mg chlorogenic acid equivalents/100 g FW for red and yellow flowers, respectively. Red flowers had the highest level of flavonoids (315.1 ± 2.4 mg myricetin equivalents/100 g FW) and the highest ORAC radical-scavenging activity. These results show the diversity and abundance of polyphenolic compounds in nasturtium flowers, which could be the basis for applications in functional foods, cosmetics, and pharmaceuticals.
[Mh] Termos MeSH primário: Cromatografia Líquida de Alta Pressão/métodos
Flores/química
Espectrometria de Massas/métodos
Fenóis/análise
Espécies Reativas de Oxigênio/química
Tropaeolum/química
[Mh] Termos MeSH secundário: Antocianinas/análise
Antioxidantes/análise
Ácidos Cumáricos/análise
Flavonoides/análise
Depuradores de Radicais Livres/química
Fenóis/química
Polifenóis/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Antioxidants); 0 (Coumaric Acids); 0 (Flavonoids); 0 (Free Radical Scavengers); 0 (Phenols); 0 (Polyphenols); 0 (Reactive Oxygen Species)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:150218
[Lr] Data última revisão:
150218
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150210
[St] Status:MEDLINE
[do] DOI:10.1021/jf503366c


  10 / 41 MEDLINE  
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[PMID]:25561232
[Au] Autor:Navarro-González I; González-Barrio R; García-Valverde V; Bautista-Ortín AB; Periago MJ
[Ad] Endereço:Department of Food Technology, Food Science and Nutrition, Faculty of Veterinary Sciences, Regional Campus of International Excellence "Campus Mare Nostrum", University of Murcia, Espinardo 30071 (Murcia), Spain. inmanago@yahoo.es.
[Ti] Título:Nutritional composition and antioxidant capacity in edible flowers: characterisation of phenolic compounds by HPLC-DAD-ESI/MSn.
[So] Source:Int J Mol Sci;16(1):805-22, 2014 Dec 31.
[Is] ISSN:1422-0067
[Cp] País de publicação:Switzerland
[La] Idioma:eng
[Ab] Resumo:Edible flowers are commonly used in human nutrition and their consumption has increased in recent years. The aim of this study was to ascertain the nutritional composition and the content and profile of phenolic compounds of three edible flowers, monks cress (Tropaeolum majus), marigold (Tagetes erecta) and paracress (Spilanthes oleracea), and to determine the relationship between the presence of phenolic compounds and the antioxidant capacity. Proximate composition, total dietary fibre (TDF) and minerals were analysed according to official methods: total phenolic compounds (TPC) were determined with Folin-Ciocalteu's reagent, whereas antioxidant capacity was evaluated using Trolox Equivalent Antioxidant Capacity (TEAC) and Oxygen Radical Absorbance Capacity (ORAC) assays. In addition, phenolic compounds were characterised by HPLC-DAD-MSn. In relation to the nutritional value, the edible flowers had a composition similar to that of other plant foods, with a high water and TDF content, low protein content and very low proportion of total fat-showing significant differences among samples. The levels of TPC compounds and the antioxidant capacity were significantly higher in T. erecta, followed by S. oleracea and T. majus. Thirty-nine different phenolic compounds were tentatively identified, with flavonols being the major compounds detected in all samples, followed by anthocyanins and hydroxycynnamic acid derivatives. In T. erecta small proportions of gallotannin and ellagic acid were also identified.
[Mh] Termos MeSH primário: Antioxidantes/química
Asteraceae/química
Fenóis/análise
Espectrometria de Massas por Ionização por Electrospray
Tagetes/química
Tropaeolum/química
[Mh] Termos MeSH secundário: Antocianinas/análise
Asteraceae/metabolismo
Cromatografia Líquida de Alta Pressão
Flavonoides/análise
Flores/química
Flores/metabolismo
Tagetes/metabolismo
Tropaeolum/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anthocyanins); 0 (Antioxidants); 0 (Flavonoids); 0 (Phenols)
[Em] Mês de entrada:1509
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150107
[St] Status:MEDLINE
[do] DOI:10.3390/ijms16010805



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