Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.962.888 [Categoria DeCS]
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  1 / 191 MEDLINE  
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[PMID]:28774309
[Au] Autor:Katoch M; Paul A; Singh G; Sridhar SNC
[Ad] Endereço:Department of Microbial Biotechnology, Indian Institute of Integrative Medicine, Canal Road, Jammu, 180001, India. meenusamiksha@rediffmail.com.
[Ti] Título:Fungal endophytes associated with Viola odorata Linn. as bioresource for pancreatic lipase inhibitors.
[So] Source:BMC Complement Altern Med;17(1):385, 2017 Aug 03.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: As per the recent statistical reports of World Health Organisation (WHO), 13% of total global population is obese. Orlistat remains to be the only drug approved for the long term treatment of obesity. Recent findings highlighted severe adverse effects of orlistat that included hepatotoxicity, gall stones, kidney stones and acute pancreatitis. Therefore, search for new drug is required. The investigations based on endophytic natural products would prove pivotal in the global fight against this health issue. METHODS: Obesity is associated with lipid metabolism involving pancreatic lipase enzyme. The inhibition of pancreatic lipase is demonstrated by using the extracts of endophytes isolated from Viola odorata Linn. In addition, endophytes were identified using ITS based rDNA sequencing. RESULTS: Present study involves the isolation and identification of 27 endophytes from V. odorata. All the endophytes were evaluated for lipase inhibitory activities. The extracts of seven endophytes exhibited lipase inhibitory activity with IC < 10 µg/mL. The extract of VOLF4 (Aspergillus sp.) displayed promising lipase inhibitory activity (IC 3.8 µg/mL). CONCLUSION: The present study demonstrates that V. odorata harbors endophytic community with potent lipase inhibitory activity. VOLF4 is the potential endophyte. The extract of VOLF4 can be used to develop the potential drug to treat obesity.
[Mh] Termos MeSH primário: Aspergillus
Produtos Biológicos/farmacologia
Endófitos
Lipase/antagonistas & inibidores
Obesidade/metabolismo
Pâncreas/enzimologia
Viola
[Mh] Termos MeSH secundário: Animais
Aspergillus/isolamento & purificação
DNA Fúngico
Endófitos/genética
Endófitos/isolamento & purificação
Lactonas/efeitos adversos
Lactonas/uso terapêutico
Metabolismo dos Lipídeos/efeitos dos fármacos
Obesidade/tratamento farmacológico
Análise de Sequência de DNA
Suínos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biological Products); 0 (DNA, Fungal); 0 (Lactones); 95M8R751W8 (orlistat); EC 3.1.1.3 (Lipase)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170901
[Lr] Data última revisão:
170901
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170805
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1893-y


  2 / 191 MEDLINE  
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[PMID]:28621949
[Au] Autor:Narayani M; Chadha A; Srivastava S
[Ad] Endereço:Department of Biotechnology, Bhupat and Jyoti Mehta School of Biosciences, Indian Institute of Technology Madras , Chennai 600036, India.
[Ti] Título:Cyclotides from the Indian Medicinal Plant Viola odorata (Banafsha): Identification and Characterization.
[So] Source:J Nat Prod;80(7):1972-1980, 2017 Jul 28.
[Is] ISSN:1520-6025
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cyclotides are cyclic cystine knotted macrocyclic plant peptides that have several promising applications. This study was undertaken to detect and identify known and new cyclotides in Viola odorata, a commercially important medicinal plant, from three geographical locations in India. The number of cyclotides in the plant varied with the tissue (leaves, petioles, flowers, runners, and roots) and with geographical locations in India. Using liquid chromatography coupled to Fourier transform mass spectrometry (FTMS), 166 cyclotide-like masses were observed to display cyclotide-diagnostic mass shifts following reduction, alkylation, and digestion, and 71 of these were positively identified based on automated spectrum matching. Of the remaining 95 putative cyclotides observed, de novo peptide sequencing of three new cyclotides, namely, vodo I1 (1), vodo I2 (2), and vodo I3 (3), was carried out with tandem mass spectrometry.
[Mh] Termos MeSH primário: Ciclotídeos/isolamento & purificação
Plantas Medicinais/química
Viola/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Cromatografia Líquida de Alta Pressão
Ciclotídeos/química
Índia
Estrutura Molecular
Ressonância Magnética Nuclear Biomolecular
Folhas de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cyclotides); 0 (vodo I1); 0 (vodo I2); 0 (vodo I3)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170915
[Lr] Data última revisão:
170915
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170617
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jnatprod.6b01004


  3 / 191 MEDLINE  
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[PMID]:28587677
[Au] Autor:Sung YY; Kim DS; Kim SH; Kim HK
[Ad] Endereço:Mibyeong Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon, 305-811, Republic of Korea.
[Ti] Título:Anti-obesity activity, acute toxicity, and chemical constituents of aqueous and ethanol Viola mandshurica extracts.
[So] Source:BMC Complement Altern Med;17(1):297, 2017 Jun 06.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Viola mandshurica has traditionally been used as an expectorant, diuretic, and anti-inflammatory drug. The present study was designed to test the hypothesis that low doses of two different V. mandshurica extracts have anti-obesity effects. METHODS: We evaluated the effects of ethanol extract (VME) and aqueous extract (VMA) from V. mandshurica on high-fat diet (HFD)-induced obese mice as well as the acute oral toxicities and chemical compositions of both extracts. RESULTS: Oral administration of VME or VMA (50, 100, or 200 mg/kg) decreased body weight gain, liver and adipose tissue mass, adipocyte size, and serum lipid levels. Both extracts increased adiponectin serum concentrations and mRNA expression in epididymal adipose tissue. VME and VMA also reversed the HFD-induced mRNA expression of lipogenic genes such as CCAAT/enhancer binding protein (C/EBP)α, C/EBPß, sterol regulatory element-binding protein 1c, and leptin in adipose tissue, whereas they increased mRNA expression of uncoupling protein 2 and adenosine monophosphate-activated protein kinase (AMPK). VME and VMA increased the phosphorylation of AMPK and acetyl-coA carboxylase with a concomitant decrease in fat accumulation in the liver. High performance liquid chromatography analysis revealed that both VME and VMA contained esculetin (0.566% for VME, 0.231% for VMA) and schaftoside (0.147% for VME, 0.126% for VMA). In a 2-week acute toxicity study, administration of a single oral dose of VME or VMA (5000 mg/kg) caused no signs of toxicity or mortality. CONCLUSIONS: These results suggest that both VM extracts exert anti-obesity effects in HFD-induced obese mice by suppressing lipogenesis and activating AMPK in the liver and adipose tissue. Our findings suggest that VM extracts could be a safe and effective treatment for obesity.
[Mh] Termos MeSH primário: Fármacos Antiobesidade/administração & dosagem
Obesidade/tratamento farmacológico
Extratos Vegetais/administração & dosagem
Viola/química
[Mh] Termos MeSH secundário: Adipócitos/efeitos dos fármacos
Adipócitos/metabolismo
Tecido Adiposo/efeitos dos fármacos
Tecido Adiposo/metabolismo
Animais
Fármacos Antiobesidade/química
Fármacos Antiobesidade/isolamento & purificação
Fármacos Antiobesidade/toxicidade
Proteínas Estimuladoras de Ligação a CCAAT/genética
Proteínas Estimuladoras de Ligação a CCAAT/metabolismo
Dieta Hiperlipídica/efeitos adversos
Seres Humanos
Leptina/metabolismo
Fígado/efeitos dos fármacos
Fígado/metabolismo
Masculino
Camundongos
Camundongos Endogâmicos C57BL
Camundongos Obesos
Obesidade/genética
Obesidade/metabolismo
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Extratos Vegetais/toxicidade
Proteína de Ligação a Elemento Regulador de Esterol 1/genética
Proteína de Ligação a Elemento Regulador de Esterol 1/metabolismo
Viola/toxicidade
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Anti-Obesity Agents); 0 (CCAAT-Enhancer-Binding Proteins); 0 (CEBPA protein, mouse); 0 (Leptin); 0 (Plant Extracts); 0 (Sterol Regulatory Element Binding Protein 1)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170821
[Lr] Data última revisão:
170821
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170608
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1810-4


  4 / 191 MEDLINE  
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[PMID]:28514904
[Au] Autor:Park SH; Sung YY; Nho KJ; Kim DS; Kim HK
[Ad] Endereço:* Mibyeong Research Center, Korea Institute of Oriental Medicine, 1672 Yuseong-daero, Yuseong-gu, Daejeon 305-811, Republic of Korea.
[Ti] Título:Effects of Viola mandshurica on Atherosclerosis and Hepatic Steatosis in ApoE[Formula: see text] via the AMPK Pathway.
[So] Source:Am J Chin Med;45(4):757-772, 2017.
[Is] ISSN:0192-415X
[Cp] País de publicação:Singapore
[La] Idioma:eng
[Ab] Resumo:Atherosclerosis was previously thought to be a disease that primarily involves lipid accumulation in the arterial wall. In this report, we investigated the effect of Viola mandshurica W. Becker (V. mandshurica) water extract on atherosclerosis in apolipoprotein E deficient (ApoE[Formula: see text]) mice. The administration of V. mandshurica to high-fat diet-fed mice reduced body weight, liver weight, and serum levels of lipids (total cholesterol, low-density lipoprotein-cholesterol, triglycerides), glucose, alanine transaminase, and aspartate transaminase. Histopathologic analyses of the aorta and liver revealed that V. mandshurica attenuated atherosclerotic lesions and reduced lipid accumulation, inflammatory responses and fatty acid synthesis. V. mandshurica also increased phosphorylation of adenosine monophosphate-activated protein kinase (AMPK), thereby reducing acetyl-CoA carboxylase (ACC) in liver tissue and inhibiting sterol regulatory element-binding protein 1c (SREBP-1c). V. mandshurica reduced protein expression levels of adhesion molecules (intercellular adhesion molecule-1, vascular cell adhesion molecule-1, and E-selectin) as well as ACC, fatty acid synthase, and SREBP-1c. In addition, quantitative analysis of V. mandshurica by high-performance liquid chromatography revealed the presence of esculetin and scopoletin. Esculetin and scopoletin reduced adhesion molecules in human aortic smooth muscle cells. Our results indicate that the anti-atherosclerotic effects of V. mandshurica may be associated with activation of the AMPK pathway. Therefore, AMPK-dependent phosphorylation of SREBP-1c by V. mandshurica may be an effective therapeutic strategy for combatting atherosclerosis and hepatic steatosis.
[Mh] Termos MeSH primário: Proteínas Quinases Ativadas por AMP/metabolismo
Aterosclerose/tratamento farmacológico
Aterosclerose/etiologia
Fígado Gorduroso/tratamento farmacológico
Fígado Gorduroso/etiologia
Terapia de Alvo Molecular
Fitoterapia
Extratos Vegetais/farmacologia
Extratos Vegetais/uso terapêutico
Viola/química
[Mh] Termos MeSH secundário: Acetil-CoA Carboxilase/metabolismo
Animais
Molécula 1 de Adesão Celular
Moléculas de Adesão Celular/metabolismo
Modelos Animais de Doenças
Imunoglobulinas/metabolismo
Metabolismo dos Lipídeos/efeitos dos fármacos
Fígado/metabolismo
Masculino
Camundongos Endogâmicos C57BL
Fosforilação/efeitos dos fármacos
Extratos Vegetais/química
Escopoletina/isolamento & purificação
Escopoletina/farmacologia
Escopoletina/uso terapêutico
Transdução de Sinais
Proteína de Ligação a Elemento Regulador de Esterol 1/metabolismo
Umbeliferonas/isolamento & purificação
Umbeliferonas/farmacologia
Umbeliferonas/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cadm1 protein, mouse); 0 (Cell Adhesion Molecule-1); 0 (Cell Adhesion Molecules); 0 (Immunoglobulins); 0 (Plant Extracts); 0 (Srebf1 protein, mouse); 0 (Sterol Regulatory Element Binding Protein 1); 0 (Umbelliferones); EC 2.7.11.31 (AMP-Activated Protein Kinases); EC 6.4.1.2 (Acetyl-CoA Carboxylase); KLF1HS0SXJ (Scopoletin); SM2XD6V944 (esculetin)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE
[do] DOI:10.1142/S0192415X17500409


  5 / 191 MEDLINE  
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[PMID]:28056938
[Au] Autor:Abdullah; Khan MA; Ahmad W; Ahmad M; Nisar M
[Ad] Endereço:Department of Pharmacy, University of Malakand, Chakdara, Pakistan.
[Ti] Título:Hepatoprotective effect of the solvent extracts of Viola canescens Wall. ex. Roxb. against CCl induced toxicity through antioxidant and membrane stabilizing activity.
[So] Source:BMC Complement Altern Med;17(1):10, 2017 Jan 05.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Viola canescens Wall. ex. Roxb. exhibits analgesic, antimalarial and antispasmodic activities. It is used folklorically for the treatment of liver diseases, hypertension, malaria and cancer. The current study investigates phytochemical constituents, antioxidant and hepatoprotective activity of solvent extracts of whole plant of Viola canescens. METHODS: Phytochemicals, acute toxicity study and antioxidant activity of Viola canescens methanolic extract (VCME), ethyl acetate fraction (EAF), and partially purified EAF (90% EAF and combination of 80% EAF + 20% methanol fraction (EAF + Me) was carried out. Hepatoprotective activity of VCME, EAF (200 and 400 mg/kg body weight) and partially purified EAF (50 mg/kg body weight) was investigated in carbon tetrachloride (CCl ) intoxicated BALB/c mice for 7 days. Membrane stabilization effect was determined by hypotonic solution induced hemolysis while DNA ladder assay was carried out by polyacrylamide gel electrophoresis. RESULTS: Phytochemical screening of VCME showed the presence of alkaloids, phenols, flavonoids, saponins, carbohydrates, tannins and triterpenes. VCME, EAF (at 200 and 400 mg/kg body weight) and partially purified EAF (90% EAF and EAF + Me) at 50 mg/kg body weight significantly reduced the level of ALT, ALP, total bilirubin and restored the level of serum protein in comparison to CCl treated group. A significant reduction in malondialdehyde (MDA) and elevation in catalase (CAT) and superoxide dismutase (SOD) level was observed in extract treated animals as compared to CCl (p < 0.05). The IC values in membrane stabilization potential for VCME, EAF and sodium salicylate were 3.7 ± 0.11, 3.4 ± 0.15 and 3.2 ± 0.09 mg/ml, respectively. Similarly, CCl induced degradation of DNA was counteracted by VCME and EAF. The liver biopsy of mice treated with the solvent extracts showed remarkable restoration of normal histological archeitecture. CONCLUSIONS: Viola canescens showed significant hepatoprotective potential due to its antioxidant and membrane stabilization effect.
[Mh] Termos MeSH primário: Antioxidantes/administração & dosagem
Membrana Celular/efeitos dos fármacos
Doença Hepática Induzida por Substâncias e Drogas/tratamento farmacológico
Extratos Vegetais/administração & dosagem
Viola/química
[Mh] Termos MeSH secundário: Animais
Antioxidantes/química
Antioxidantes/isolamento & purificação
Tetracloreto de Carbono/toxicidade
Membrana Celular/fisiologia
Doença Hepática Induzida por Substâncias e Drogas/metabolismo
Seres Humanos
Fígado/efeitos dos fármacos
Fígado/metabolismo
Masculino
Malondialdeído/metabolismo
Potenciais da Membrana/efeitos dos fármacos
Camundongos
Camundongos Endogâmicos BALB C
Extratos Vegetais/química
Extratos Vegetais/isolamento & purificação
Superóxido Dismutase/metabolismo
Viola/efeitos adversos
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antioxidants); 0 (Plant Extracts); 4Y8F71G49Q (Malondialdehyde); CL2T97X0V0 (Carbon Tetrachloride); EC 1.15.1.1 (Superoxide Dismutase)
[Em] Mês de entrada:1701
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170107
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-016-1537-7


  6 / 191 MEDLINE  
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[PMID]:27743255
[Au] Autor:Komatsu K; Yamashita K; Sugawara K; Verbeek M; Fujita N; Hanada K; Uehara-Ichiki T; Fuji SI
[Ad] Endereço:Laboratory of Plant Pathology, Graduate School of Agriculture, Tokyo University of Agriculture and Technology (TUAT), 3-5-8 Saiwaicho, Fuchu, Tokyo, 183-8509, Japan.
[Ti] Título:Complete genome sequences of two highly divergent Japanese isolates of Plantago asiatica mosaic virus.
[So] Source:Arch Virol;162(2):581-584, 2017 Feb.
[Is] ISSN:1432-8798
[Cp] País de publicação:Austria
[La] Idioma:eng
[Ab] Resumo:Plantago asiatica mosaic virus (PlAMV) is a member of the genus Potexvirus and has an exceptionally wide host range. It causes severe damage to lilies. Here we report on the complete nucleotide sequences of two new Japanese PlAMV isolates, one from the eudicot weed Viola grypoceras (PlAMV-Vi), and the other from the eudicot shrub Nandina domestica Thunb. (PlAMV-NJ). Their genomes contain five open reading frames (ORFs), which is characteristic of potexviruses. Surprisingly, the isolates showed only 76.0-78.0 % sequence identity with each other and with other PlAMV isolates, including isolates from Japanese lily and American nandina. Amino acid alignments of the replicase coding region encoded by ORF1 showed that the regions between the methyltransferase and helicase domains were less conserved than other regions, with several insertions and/or deletions. Phylogenetic analyses of the full-length nucleotide sequences revealed a moderate correlation between phylogenetic clustering and the original host plants of the PlAMV isolates. This study revealed the presence of two highly divergent PlAMV isolates in Japan.
[Mh] Termos MeSH primário: Genoma Viral
Vírus do Mosaico/genética
Filogenia
Potexvirus/genética
Proteínas Virais/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Sequência de Bases
Berberidaceae/virologia
Mapeamento Cromossômico
Mutação INDEL
Japão
Metiltransferases/genética
Vírus do Mosaico/classificação
Vírus do Mosaico/isolamento & purificação
Fases de Leitura Aberta
Potexvirus/classificação
Potexvirus/isolamento & purificação
RNA Helicases/genética
Alinhamento de Sequência
Viola/virologia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Viral Proteins); EC 2.1.1.- (Methyltransferases); EC 3.6.4.13 (RNA Helicases)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170214
[Lr] Data última revisão:
170214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161016
[St] Status:MEDLINE
[do] DOI:10.1007/s00705-016-3110-6


  7 / 191 MEDLINE  
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[PMID]:27870888
[Au] Autor:Casazza G; Grassi F; Zecca G; Minuto L
[Ad] Endereço:DISTAV, Università degli studi di Genova, Genova, Italy.
[Ti] Título:Phylogeographic Insights into a Peripheral Refugium: The Importance of Cumulative Effect of Glaciation on the Genetic Structure of Two Endemic Plants.
[So] Source:PLoS One;11(11):e0166983, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Quaternary glaciations and mostly last glacial maximum have shaped the contemporary distribution of many species in the Alps. However, in the Maritime and Ligurian Alps a more complex picture is suggested by the presence of many Tertiary paleoendemisms and by the divergence time between lineages in one endemic species predating the Late Pleistocene glaciation. The low number of endemic species studied limits the understanding of the processes that took place within this region. We used species distribution models and phylogeographical methods to infer glacial refugia and to reconstruct the phylogeographical pattern of Silene cordifolia All. and Viola argenteria Moraldo & Forneris. The predicted suitable area for last glacial maximum roughly fitted current known distribution. Our results suggest that separation of the major clades predates the last glacial maximum and the following repeated glacial and interglacial periods probably drove differentiations. The complex phylogeographical pattern observed in the study species suggests that both populations and genotypes extinction was minimal during the last glacial maximum, probably due to the low impact of glaciations and to topographic complexity in this area. This study underlines the importance of cumulative effect of previous glacial cycles in shaping the genetic structure of plant species in Maritime and Ligurian Alps, as expected for a Mediterranean mountain region more than for an Alpine region.
[Mh] Termos MeSH primário: Camada de Gelo
Silene/genética
Viola/genética
[Mh] Termos MeSH secundário: Filogeografia
Silene/crescimento & desenvolvimento
Viola/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170623
[Lr] Data última revisão:
170623
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161122
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0166983


  8 / 191 MEDLINE  
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[PMID]:27687791
[Au] Autor:Qin Y; Pang Y; Cheng Z
[Ad] Endereço:Department of Pharmacognosy, School of Pharmacy, Fudan University, 826 Zhangheng Road, Shanghai, 201203, China.
[Ti] Título:Needle Trap Device as a New Sampling and Preconcentration Approach for Volatile Organic Compounds of Herbal Medicines and its Application to the Analysis of Volatile Components in Viola tianschanica.
[So] Source:Phytochem Anal;27(6):364-374, 2016 Nov.
[Is] ISSN:1099-1565
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:INTRODUCTION: The needle trap device (NTD) technique is a new microextraction method for sampling and preconcentration of volatile organic compounds (VOCs). Previous NTD studies predominantly focused on analysis of environmental volatile compounds in the gaseous and liquid phases. Little work has been done on its potential application in biological samples and no work has been reported on analysis of bioactive compounds in essential oils from herbal medicines. OBJECTIVE: The main purpose of the present study is to develop a NTD sampling method for profiling VOCs in biological samples using herbal medicines as a case study. METHODOLOGY: A combined method of NTD sample preparation and gas chromatography-mass spectrometry was developed for qualitative analysis of VOCs in Viola tianschanica. A 22-gauge stainless steel, triple-bed needle packed with Tenax, Carbopack X and Carboxen 1000 sorbents was used for analysis of VOCs in the herb. Furthermore, different parameters affecting the extraction efficiency and capacity were studied. RESULTS: The peak capacity obtained by NTDs was 104, more efficient than those of the static headspace (46) and hydrodistillation (93). This NTD method shows potential to trap a wide range of VOCs including the lower and higher volatile components, while the static headspace and hydrodistillation only detects lower volatile components, and semi-volatile and higher volatile components, respectively. CONCLUSION: The developed NTD sample preparation method is a more rapid, simpler, convenient, and sensitive extraction/desorption technique for analysis of VOCs in herbal medicines than the conventional methods such as static headspace and hydrodistillation. Copyright © 2016 John Wiley & Sons, Ltd.
[Mh] Termos MeSH primário: Medicina Herbária
Agulhas
Viola/química
Compostos Orgânicos Voláteis/análise
[Mh] Termos MeSH secundário: Cromatografia Gasosa-Espectrometria de Massas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Volatile Organic Compounds)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170227
[Lr] Data última revisão:
170227
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161001
[St] Status:MEDLINE
[do] DOI:10.1002/pca.2636


  9 / 191 MEDLINE  
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[PMID]:27403748
[Au] Autor:Niyomploy P; Chan LY; Poth AG; Colgrave ML; Sangvanich P; Craik DJ
[Ad] Endereço:Institute for Molecular Bioscience, The University of Queensland, Brisbane, QLD, 4072, Australia.
[Ti] Título:Discovery, isolation, and structural characterization of cyclotides from Viola sumatrana Miq.
[So] Source:Biopolymers;106(6):796-805, 2016 Nov.
[Is] ISSN:1097-0282
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Cyclotides are cyclic peptides from plants in the Violaceae, Rubiaceae, Fabaceae, Cucurbitaceae, and Solanaceae families. They are sparsely distributed in most of these families, but appear to be ubiquitous in the Violaceae, having been found in every plant so far screened from this family. However, not all geographic regions have been examined and here we report the discovery of cyclotides from a Viola species from South-East Asia. Two novel cyclotides (Visu 1 and Visu 2) and two known cyclotides (kalata S and kalata B1) were identified in V. sumatrana. NMR studies revealed that kalata S and kalata B1 had similar secondary structures. Their biological activities were determined in cytotoxicity assays; both had similar cytotoxic activity and were more toxic to U87 cells compared with other cell lines. Overall, the study strongly supports the ubiquity of cyclotides in the Violaceae and adds to our understanding of their distribution and cytotoxic activity.
[Mh] Termos MeSH primário: Citotoxinas
Peptídeos Cíclicos
Proteínas de Plantas
Viola/química
[Mh] Termos MeSH secundário: Linhagem Celular
Citotoxinas/química
Citotoxinas/isolamento & purificação
Citotoxinas/farmacologia
Seres Humanos
Peptídeos Cíclicos/química
Peptídeos Cíclicos/isolamento & purificação
Peptídeos Cíclicos/farmacologia
Proteínas de Plantas/química
Proteínas de Plantas/isolamento & purificação
Proteínas de Plantas/farmacologia
Estrutura Secundária de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Cytotoxins); 0 (Peptides, Cyclic); 0 (Plant Proteins)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:170206
[Lr] Data última revisão:
170206
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160713
[St] Status:MEDLINE
[do] DOI:10.1002/bip.22914


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[PMID]:27394154
[Au] Autor:Slazak B; Kapusta M; Malik S; Bohdanowicz J; Kuta E; Malec P; Göransson U
[Ad] Endereço:W. Szafer Institute of Botany, Polish Academy of Science, 46 Lubicz St, 31-512, Cracow, Poland. b.slazak@botany.pl.
[Ti] Título:Immunolocalization of cyclotides in plant cells, tissues and organ supports their role in host defense.
[So] Source:Planta;244(5):1029-1040, 2016 Nov.
[Is] ISSN:1432-2048
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:MAIN CONCLUSION: The distribution of cyclotides was visualized in plant cells, tissues and organs using immunohistochemistry. Finding of cyclotides in tissues potentially vulnerable to pathogen attacks supports their role as defense molecules. The cyclotide family of plant peptides is characterized by the cyclic cystine knot motif and its diverse biological activities. Given their insecticidal and antimicrobial properties, the role of cyclotides in planta is probably associated with host defense. Our current understanding of the cellular compartmentalization of cyclotides in the vacuole is based on indirect studies on transgenic model plants that do not express cyclotides naturally. Matrix-assisted laser desorption ionization (MALDI) imaging has also been used to study the distribution of cyclotides, but the technique's resolution was insufficient to determine their tissue or cell distribution. To avoid the limitations of these approaches, immunohistochemical visualization methods were used. Antibodies were raised in rabbits using cycloviolacin O2 (cyO2), and their specificity was determined by Western and dot blot experiments. Slides for immunohistochemical analysis were prepared from leaf, petiole and root fragments of Viola odorata and Viola uliginosa, and specimens were visualized using indirect epifluorescence microscopy. The antibodies against cyclotides were specific against selected bracelet cyclotides with high similarity (cyO2, cyO3, cyO8, cyO13) and suitable for immunohistochemistry. The tissue distribution of the cyclotides visualized in this way is consistent with their proposed role in host defense-relatively large quantities were observed in the leaf and petiole epidermis in both Viola species. Cyclotides were also found in vascular tissue in all the assessed plant organs. The vacuole storage of cyclotides was directly shown.
[Mh] Termos MeSH primário: Ciclotídeos/metabolismo
Especificidade de Órgãos
Células Vegetais/metabolismo
Imunidade Vegetal
Viola/imunologia
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Anticorpos/metabolismo
Especificidade de Anticorpos/imunologia
Compartimento Celular
Ciclotídeos/biossíntese
Ciclotídeos/química
Imuno-Histoquímica
Folhas de Planta/metabolismo
Ligação Proteica
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antibodies); 0 (Cyclotides)
[Em] Mês de entrada:1704
[Cu] Atualização por classe:170403
[Lr] Data última revisão:
170403
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160711
[St] Status:MEDLINE



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