Base de dados : MEDLINE
Pesquisa : B01.650.940.800.575.912.250.965 [Categoria DeCS]
Referências encontradas : 98 [refinar]
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  1 / 98 MEDLINE  
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[PMID]:27393430
[Au] Autor:Lin Z; Chen L; Qiu Q; Guo S
[Ad] Endereço:State Key Laboratory of Chinese Pharmacies, Pharmacy College, Fujian University of Traditional Chinese Medicine, Fuzhou, China.
[Ti] Título:Isolation and identification of antiproliferative compounds from the roots of Tetrastigma hemsleyanum against MDA-MB-435S cell lines.
[So] Source:Pak J Pharm Sci;29(4):1171-5, 2016 Jul.
[Is] ISSN:1011-601X
[Cp] País de publicação:Pakistan
[La] Idioma:eng
[Ab] Resumo:This present study aimed to elucidate antiproliferative activity of four extracts (CHCl(3), EtOAc, n-BuOH and H(2)O) and chemical constituents isolated from the most potent extract of Tetrastigma hemsleyanum Diels et. Gilg (TDG) against MDA-MB-435S cell lines using the MTT assay at various concentrations in vitro. Ten compounds were isolated and identified as (1) ß-sitosterol, (2) palmitic acid, (3) protocatechuic acid, (4) salicylic acid, (5) p-hydroxybenzoic acid, (6) resveratrol, (7) trans-4-hydroxycinnamic acid, (8) kaempferol, (9) quercetin, and (10) isoquercitrin. Compounds 3, 5-7, 10 were the first report of isolation from this plant. Moreover, antiproliferative activity displayed that the CHCl(3), H(2)O extracts and compounds 6, 8 exhibited obvious inhibitory effects on MDA-MB-435S cell lines with IC(50) values 100.28± 2.64, 127.48±3.45, 92.39±1.68 and 120.30±1.97µ/mL, respectively. Thus the obtained results indicate antiproliferative activity of TDG against MDA-MB-435S cell lines is ascribable to the most potent CHCl(3) extract along with active compounds 6 and 8, which could be considered as a potential chemotherapeutic agent in breast cancer.
[Mh] Termos MeSH primário: Antineoplásicos Fitogênicos/isolamento & purificação
Vitaceae/química
[Mh] Termos MeSH secundário: Antineoplásicos Fitogênicos/farmacologia
Linhagem Celular Tumoral
Seres Humanos
Extratos Vegetais/farmacologia
Raízes de Plantas/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antineoplastic Agents, Phytogenic); 0 (Plant Extracts)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160709
[Lr] Data última revisão:
160709
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160710
[St] Status:MEDLINE


  2 / 98 MEDLINE  
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[PMID]:27245064
[Au] Autor:Peng X; Wu X; Ji Q; Yang R; Li Y
[Ad] Endereço:Institute of Biopharmaceutical Technology, Zhejiang Pharmaceutical College, Ningbo, 315100, Zhejiang, China.
[Ti] Título:Molecular authentication of Tetrastigma hemsleyanum from its adulterant species using ISSR, CAPS, and ITS2 barcode.
[So] Source:Mol Biol Rep;43(8):785-94, 2016 Aug.
[Is] ISSN:1573-4978
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Tetrastigma hemsleyanum is a rare and endangered herb, which is commercialized as the resource of anti-cancer drugs. Wild T. hemsleyanum plants are on the verge of extinction recently, there are increasing numbers of counterfeits on the market. In the present study, inter-simple sequence repeat (ISSR), Cleaved amplified polymorphic sequence (CAPS), and the internal transcribed spacer region II (ITS2) barcode were used for the first time for the authentication of T. hemsleyanum from its commonly counterfeits. ISSR analysis suggested that it was a useful method for distinguishing T. hemsleyanum from its adulterants of different genus. However, it was insufficient to distinguish T. hemsleyanum from those adulterants of the same genus. ITS2 of T. hemsleyanum and the commonly counterfeits were amplified and sequenced. The Neighbor-Joining tree constructed from the ITS2 sequences showed that T. hemsleyanum was clearly differentiated from all counterfeits samples. A mutation site in the ITS2 region of T. hemsleyanum had been found which could be recognized by the restriction endonuclease NcoI. T. hemsleyanum could be readily distinguished from counterfeits as the PCR products from T. hemsleyanum could be digested sufficiently by NcoI, while the PCR products from counterfeits could not be digested. The results indicated that CAPS and ITS2 barcode methods provided effective and accurate identification of T. hemsleyanum from all its adulterants, while ISSR could only distinguish T. hemsleyanum from its adulterants of different genus. The CAPS method developed in the present study will serve as a reliable tool for safe and effective use of T. hemsleyanum in the clinic application. It will also play an important role for the identification, management and conservation of this endangered species.
[Mh] Termos MeSH primário: Espécies em Perigo de Extinção
Vitaceae/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Código de Barras de DNA Taxonômico
DNA de Plantas/genética
DNA Espaçador Ribossômico/genética
Medicamentos de Ervas Chinesas
Genes de Plantas
Repetições de Microssatélites
Filogenia
Polimorfismo Genético
Vitaceae/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Plant); 0 (DNA, Ribosomal Spacer); 0 (Drugs, Chinese Herbal)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171030
[Lr] Data última revisão:
171030
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160602
[St] Status:MEDLINE
[do] DOI:10.1007/s11033-016-4023-x


  3 / 98 MEDLINE  
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[PMID]:27053227
[Au] Autor:Huang L; Tang X; Zhang W; Jiang R; Chen D; Zhang J; Zhong H
[Ad] Endereço:Mass Spectrometry Center for Structural Identification of Biological Molecules and Precision Medicine, Key Laboratory of Pesticides and Chemical Biology, Ministry of Education, College of Chemistry, Central China Normal University, Wuhan, Hubei 430079, P. R. China.
[Ti] Título:Imaging of Endogenous Metabolites of Plant Leaves by Mass Spectrometry Based on Laser Activated Electron Tunneling.
[So] Source:Sci Rep;6:24164, 2016 Apr 07.
[Is] ISSN:2045-2322
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A new mass spectrometric imaging approach based on laser activated electron tunneling (LAET) was described and applied to analysis of endogenous metabolites of plant leaves. LAET is an electron-directed soft ionization technique. Compressed thin films of semiconductor nanoparticles of bismuth cobalt zinc oxide were placed on the sample plate for proof-of-principle demonstration because they can not only absorb ultraviolet laser but also have high electron mobility. Upon laser irradiation, electrons are excited from valence bands to conduction bands. With appropriate kinetic energies, photoexcited electrons can tunnel away from the barrier and eventually be captured by charge deficient atoms present in neutral molecules. Resultant unpaired electron subsequently initiates specific chemical bond cleavage and generates ions that can be detected in negative ion mode of the mass spectrometer. LAET avoids the co-crystallization process of routinely used organic matrix materials with analyzes in MALDI (matrix assisted-laser desorption ionization) analysis. Thus uneven distribution of crystals with different sizes and shapes as well as background peaks in the low mass range resulting from matrix molecules is eliminated. Advantages of LAET imaging technique include not only improved spatial resolution but also photoelectron capture dissociation which produces predictable fragment ions.
[Mh] Termos MeSH primário: Elétrons
Lasers
Folhas de Planta/metabolismo
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz/métodos
[Mh] Termos MeSH secundário: Ácido Abscísico/metabolismo
Bismuto/química
Ácido Cítrico/metabolismo
Cobalto/química
Cristalização
Ciclopentanos/metabolismo
Ácidos Graxos/metabolismo
Giberelinas/metabolismo
Metabolômica/métodos
Nanopartículas Metálicas/química
Óxidos/química
Oxilipinas/metabolismo
Semicondutores
Vitaceae/metabolismo
Óxido de Zinco/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Cyclopentanes); 0 (Fatty Acids); 0 (Gibberellins); 0 (Oxides); 0 (Oxylipins); 2968PHW8QP (Citric Acid); 3G0H8C9362 (Cobalt); 6RI5N05OWW (jasmonic acid); 72S9A8J5GW (Abscisic Acid); A6I4E79QF1 (bismuth oxide); BU0A7MWB6L (gibberellic acid); SOI2LOH54Z (Zinc Oxide); U015TT5I8H (Bismuth); USK772NS56 (cobalt oxide)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170310
[Lr] Data última revisão:
170310
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160408
[St] Status:MEDLINE
[do] DOI:10.1038/srep24164


  4 / 98 MEDLINE  
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[PMID]:26586173
[Au] Autor:Higa T; Wada M
[Ad] Endereço:Department of Biological Sciences, Graduate School of Science and Engineering, Tokyo Metropolitan University, Tokyo, 192-0397, Japan.
[Ti] Título:Chloroplast avoidance movement is not functional in plants grown under strong sunlight.
[So] Source:Plant Cell Environ;39(4):871-82, 2016 Apr.
[Is] ISSN:1365-3040
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Chloroplast movement in nine climbing plant species was investigated. It is thought that chloroplasts generally escape from strong light to avoid photodamage but accumulate towards weak light to perform photosynthesis effectively. Unexpectedly, however, the leaves of climbing plants grown under strong sunlight showed very low or no chloroplast photorelocation responses to either weak or strong blue light when detected by red light transmittance through leaves. Direct observations of Cayratia japonica leaves, for example, revealed that the average number of chloroplasts in upper periclinal walls of palisade tissue cells was only 1.2 after weak blue-light irradiation and almost all of the chloroplasts remained at the anticlinal wall, the state of chloroplast avoidance response. The leaves grown under strong light have thin and columnar palisade tissue cells comparing with the leaves grown under low light. Depending on our analyses and our schematic model, the thinner cells in a unit leaf area have a wider total plasma membrane area, such that more chloroplasts can exist on the plasma membrane in the thinner cells than in the thicker cells in a unit leaf-area basis. The same strategy might be used in other plant leaves grown under direct sunlight.
[Mh] Termos MeSH primário: Cloroplastos/efeitos da radiação
Luz Solar
Vitaceae/crescimento & desenvolvimento
Vitaceae/efeitos da radiação
[Mh] Termos MeSH secundário: Forma Celular/efeitos da radiação
Clorofila/metabolismo
Células do Mesofilo/citologia
Células do Mesofilo/efeitos da radiação
Microscopia de Fluorescência
Tamanho do Órgão
Folhas de Planta/anatomia & histologia
Folhas de Planta/fisiologia
Folhas de Planta/efeitos da radiação
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
1406-65-1 (Chlorophyll)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151121
[St] Status:MEDLINE
[do] DOI:10.1111/pce.12681


  5 / 98 MEDLINE  
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[PMID]:26545592
[Au] Autor:Liu XQ; Ickert-Bond SM; Nie ZL; Zhou Z; Chen LQ; Wen J
[Ad] Endereço:Key Laboratory of Horticultural Plant Biology (Ministry of Education), College of Horticulture and Forestry Science, Huazhong Agricultural University, Wuhan 430070, China.
[Ti] Título:Phylogeny of the Ampelocissus-Vitis clade in Vitaceae supports the New World origin of the grape genus.
[So] Source:Mol Phylogenet Evol;95:217-28, 2016 Feb.
[Is] ISSN:1095-9513
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The grapes and the close allies in Vitaceae are of great agronomic and economic importance. Our previous studies showed that the grape genus Vitis was closely related to three tropical genera, which formed the Ampelocissus-Vitis clade (including Vitis, Ampelocissus, Nothocissus and Pterisanthes). Yet the phylogenetic relationships of the four genera within this clade remain poorly resolved. Furthermore, the geographic origin of Vitis is still controversial, because the sampling of the close relatives of Vitis was too limited in the previous studies. This study reconstructs the phylogenetic relationships within the clade, and hypothesizes the origin of Vitis in a broader phylogenetic framework, using five plastid and two nuclear markers. The Ampelocissus-Vitis clade is supported to be composed of five main lineages. Vitis includes two described subgenera each as a monophyletic group. Ampelocissus is paraphyletic. The New World Ampelocissus does not form a clade and shows a complex phylogenetic relationship, with A. acapulcensis and A. javalensis forming a clade, and A. erdvendbergiana sister to Vitis. The majority of the Asian Ampelocissus species form a clade, within which Pterisanthes is nested. Pterisanthes is polyphyletic, suggesting that the lamellate inflorescence characteristic of the genus represents convergence. Nothocissus is sister to the clade of Asian Ampelocissus and Pterisanthes. The African Ampelocissus forms a clade with several Asian species. Based on the Bayesian dating and both the RASP and Lagrange analyses, Vitis is inferred to have originated in the New World during the late Eocene (39.4Ma, 95% HPD: 32.6-48.6Ma), then migrated to Eurasia in the late Eocene (37.3Ma, 95% HPD: 30.9-45.1Ma). The North Atlantic land bridges (NALB) are hypothesized to be the most plausible route for the Vitis migration from the New World to Eurasia, while intercontinental long distance dispersal (LDD) cannot be eliminated as a likely mechanism.
[Mh] Termos MeSH primário: Vitaceae/classificação
Vitis/classificação
Vitis/genética
[Mh] Termos MeSH secundário: Teorema de Bayes
Especiação Genética
Filogenia
Filogeografia
Plastídeos/genética
Análise de Sequência de DNA
Vitaceae/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1609
[Cu] Atualização por classe:160122
[Lr] Data última revisão:
160122
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151108
[St] Status:MEDLINE


  6 / 98 MEDLINE  
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[PMID]:26254612
[Au] Autor:Peng X; Zhang YY; Wang J; Ji Q
[Ad] Endereço:Institute of Biopharmaceutical, Zhejiang Pharmaceutical College, Ningbo, 315100, Zhejiang, China. px4142@163.com.
[Ti] Título:Ethylacetate extract from Tetrastigma hemsleyanum induces apoptosis via the mitochondrial caspase-dependent intrinsic pathway in HepG2 cells.
[So] Source:Tumour Biol;37(1):865-76, 2016 Jan.
[Is] ISSN:1423-0380
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Ethylacetate extract of Tetrastigma hemsleyanum (EET) has a potent antitumor activity in vitro and in vivo. However, the molecular mechanism underlying EET-induced apoptosis remains elusive. As part of our continuing studies, we investigated the apoptosis mechanism of HepG2 cells exposed to different concentrations of EET in vitro. Confocal laser scanning was used to detect the apoptotic morphological changes. Flow cytometer and inverted fluorescence microscope were used to detect the mitochondrial membrane potential and cytosolic Ca(2+) level. Western blotting analysis was used to evaluate the expression of the apoptosis-related proteins. Annexin V/PI staining was used to investigate cell apoptosis. Spectrophotometry was used to detect the activity of caspase family. The results showed that distinct apoptotic morphological changes occurred in HepG2 cells treated by EET. EET caused collapse of mitochondrial membrane potential, elevation of cytosolic Ca(2+) level, and evoked release of cytochrome c from mitochondria in a concentration-dependent manner. The apoptosis was accompanied by a significant activation of caspase-3, caspase-9, and the cleavage of poly (ADP-ribose) polymerase, but there was no significant change in either the activity or the expression level of caspase-8. Furthermore, EET-induced apoptosis could be inhibited by caspase-9 inhibitor Z-LEHD-FMK but not by caspase-8 inhibitor Z-IETD-FMK. Taken together, these overall results demonstrated that EET-induced apoptosis of HepG2 cells was mediated by the mitochondrial caspase-dependent intrinsic pathway rather than the death receptor/caspase-8-mediated signaling route.
[Mh] Termos MeSH primário: Apoptose
Caspases/metabolismo
Citocromos c/metabolismo
Potencial da Membrana Mitocondrial
Mitocôndrias/metabolismo
Extratos Vegetais/química
[Mh] Termos MeSH secundário: Acetatos/química
Cálcio/metabolismo
Caspase 3/metabolismo
Caspase 8/metabolismo
Caspase 9/metabolismo
Corantes Fluorescentes/química
Células Hep G2
Seres Humanos
Vitaceae/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Fluorescent Dyes); 0 (Plant Extracts); 76845O8NMZ (ethyl acetate); 9007-43-6 (Cytochromes c); EC 3.4.22.- (CASP3 protein, human); EC 3.4.22.- (CASP8 protein, human); EC 3.4.22.- (CASP9 protein, human); EC 3.4.22.- (Caspase 3); EC 3.4.22.- (Caspase 8); EC 3.4.22.- (Caspase 9); EC 3.4.22.- (Caspases); SY7Q814VUP (Calcium)
[Em] Mês de entrada:1702
[Cu] Atualização por classe:171111
[Lr] Data última revisão:
171111
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150810
[St] Status:MEDLINE
[do] DOI:10.1007/s13277-015-3579-8


  7 / 98 MEDLINE  
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[PMID]:25801225
[Au] Autor:Khan R; Saif AQ; Quradha MM; Ali J; Rauf A; Khan A
[Ad] Endereço:a Institute of Chemical Sciences, University of Peshawar , Peshawar 25120 , KPK , Pakistan.
[Ti] Título:Antioxidant, antimicrobial and urease inhibiting activities of methanolic extracts from Cyphostemma digitatum stem and roots.
[So] Source:Nat Prod Res;30(4):486-8, 2016.
[Is] ISSN:1478-6427
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Cyphostemma digitatum stem and roots extracts were investigated for antioxidant, antimicrobial, urease inhibition potential and phytochemical analysis. Phytochemical screening of the roots and stem extract revealed the presence of secondary metabolites including flavonoids, alkaloids, coumarins, saponins, terpenoids, tannins, carbohydrates/reducing sugars and phenolic compounds. The methanolic extracts of the roots displayed highest antioxidant activity (93.518%) against DPPH while the crude methanolic extract of the stem showed highest antioxidant activity (66.163%) at 100 µg/mL concentration. The methanolic extracts of both stem and roots were moderately active or even found to be less active against the selected bacterial and fungal strains (Tables S2 and S3). The roots extract (methanol) showed significant urease enzyme inhibition activity (IC50 = 41.2 ± 0.66; 0.2 mg/mL) while the stem extract was found moderately active (IC50 = 401.1 ± 0.58; 0.2 mg/mL) against thiourea (IC50 = 21.011; 0.2 mg/mL).
[Mh] Termos MeSH primário: Anti-Infecciosos/farmacologia
Antioxidantes/farmacologia
Extratos Vegetais/química
Urease/antagonistas & inibidores
Vitaceae/química
[Mh] Termos MeSH secundário: Anti-Infecciosos/isolamento & purificação
Antioxidantes/isolamento & purificação
Testes de Sensibilidade Microbiana
Compostos Fitoquímicos/isolamento & purificação
Compostos Fitoquímicos/farmacologia
Raízes de Plantas/química
Caules de Planta/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Anti-Infective Agents); 0 (Antioxidants); 0 (Phytochemicals); 0 (Plant Extracts); EC 3.5.1.5 (Urease)
[Em] Mês de entrada:1610
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150325
[St] Status:MEDLINE
[do] DOI:10.1080/14786419.2015.1023726


  8 / 98 MEDLINE  
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[PMID]:26311669
[Au] Autor:Fukano Y; Yamawo A
[Ti] Título:Self-discrimination in the tendrils of the vine Cayratia japonica is mediated by physiological connection.
[So] Source:Proc Biol Sci;282(1814), 2015 Sep 07.
[Is] ISSN:1471-2954
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Although self-discrimination has been well documented, especially in animals, self-discrimination in plants has been identified in only a few cases, such as self-incompatibility in flowers and root discrimination. Here, were port a new form of self-discrimination in plants: discrimination by vine tendrils. We found that tendrils of the perennial vine Cayratia japonica were more likely to coil around neighbouring non-self plants than neighbouring self plants in both experimental and natural settings. The higher level of coiling around a physiologically severed self plant compared with that around a physiologically connected self plant suggested that self-discrimination was mediated by physiological coordination between the tendril and the touched plant as reported for self-discrimination in roots. The results highlight the importance of self-discrimination for plant competition not only underground,but also above-ground.
[Mh] Termos MeSH primário: Componentes Aéreos da Planta/fisiologia
Vitaceae/fisiologia
[Mh] Termos MeSH secundário: Componentes Aéreos da Planta/crescimento & desenvolvimento
Desenvolvimento Vegetal
Vitaceae/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150828
[St] Status:MEDLINE
[do] DOI:10.1098/rspb.2015.1379


  9 / 98 MEDLINE  
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[PMID]:26221590
[Au] Autor:Akhter S; Rahman MA; Aklima J; Hasan MR; Chowdhury JM
[Ad] Endereço:Department of Biochemistry and Molecular Biology, University of Chittagong, Chittagong 4331, Bangladesh.
[Ti] Título:Antioxidative Role of Hatikana (Leea macrophylla Roxb.) Partially Improves the Hepatic Damage Induced by CCl4 in Wistar Albino Rats.
[So] Source:Biomed Res Int;2015:356729, 2015.
[Is] ISSN:2314-6141
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:This research investigated the protective role of Leea macrophylla extract on CCl4-induced acute liver injury in rats. Different fractions of Leea macrophylla (Roxb.) crude extract were subjected to analysis for antioxidative effects. Rats were randomly divided into four groups as normal control, hepatic control, and reference control (silymarin) group and treatment group. Evaluations were made for the effects of the fractions on serum enzymes and biochemical parameters of CCl4-induced albino rat. Histopathological screening was also performed to evaluate the changes of liver tissue before and after treatment. Different fractions of Leea macrophylla showed very potent 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging effect, FeCl3 reducing effect, superoxide scavenging effect, and iron chelating effect. Carbon tetrachloride induction increased the level of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) and other biochemical parameters such as lipid profiles, total protein, and CK-MB. In contrast, treatment of Leea macrophylla reduced the serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP) activities as well as biochemical parameters activities. L. macrophylla partially restored the lipid profiles, total protein, and CK-MB. Histopathology showed the treated liver towards restoration. Results evidenced that L. macrophylla can be prospective source of hepatic management in liver injury.
[Mh] Termos MeSH primário: Antioxidantes/farmacologia
Fígado/patologia
Vitaceae/química
[Mh] Termos MeSH secundário: Alanina Transaminase/metabolismo
Fosfatase Alcalina/metabolismo
Animais
Compostos de Bifenilo/química
Tetracloreto de Carbono
Cloretos/química
Colesterol/metabolismo
Creatina Quinase Forma MB/metabolismo
Dimetil Sulfóxido/química
Feminino
Compostos Férricos/química
Depuradores de Radicais Livres/química
Concentração Inibidora 50
Quelantes de Ferro/farmacologia
Fígado/efeitos dos fármacos
Masculino
Picratos/química
Extratos Vegetais/farmacologia
Folhas de Planta/química
Ratos Wistar
Análise de Regressão
Superóxidos/química
Testes de Toxicidade Aguda
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antioxidants); 0 (Biphenyl Compounds); 0 (Chlorides); 0 (Ferric Compounds); 0 (Free Radical Scavengers); 0 (Iron Chelating Agents); 0 (Picrates); 0 (Plant Extracts); 11062-77-4 (Superoxides); 97C5T2UQ7J (Cholesterol); CL2T97X0V0 (Carbon Tetrachloride); DFD3H4VGDH (1,1-diphenyl-2-picrylhydrazyl); EC 2.6.1.2 (Alanine Transaminase); EC 2.7.3.2 (Creatine Kinase, MB Form); EC 3.1.3.1 (Alkaline Phosphatase); U38V3ZVV3V (ferric chloride); YOW8V9698H (Dimethyl Sulfoxide)
[Em] Mês de entrada:1605
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150730
[St] Status:MEDLINE
[do] DOI:10.1155/2015/356729


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[PMID]:26086805
[Au] Autor:Wang Q; Liu Z; Ziegler SI; Shi K
[Ad] Endereço:Department of Nuclear Medicine, Klinikum Rechts der Isar, Technische Universität München, Munich, Germany.
[Ti] Título:Enhancing spatial resolution of (18)F positron imaging with the Timepix detector by classification of primary fired pixels using support vector machine.
[So] Source:Phys Med Biol;60(13):5261-78, 2015 Jul 07.
[Is] ISSN:1361-6560
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Position-sensitive positron cameras using silicon pixel detectors have been applied for some preclinical and intraoperative clinical applications. However, the spatial resolution of a positron camera is limited by positron multiple scattering in the detector. An incident positron may fire a number of successive pixels on the imaging plane. It is still impossible to capture the primary fired pixel along a particle trajectory by hardware or to perceive the pixel firing sequence by direct observation. Here, we propose a novel data-driven method to improve the spatial resolution by classifying the primary pixels within the detector using support vector machine. A classification model is constructed by learning the features of positron trajectories based on Monte-Carlo simulations using Geant4. Topological and energy features of pixels fired by (18)F positrons were considered for the training and classification. After applying the classification model on measurements, the primary fired pixels of the positron tracks in the silicon detector were estimated. The method was tested and assessed for [(18)F]FDG imaging of an absorbing edge protocol and a leaf sample. The proposed method improved the spatial resolution from 154.6 ± 4.2 µm (energy weighted centroid approximation) to 132.3 ± 3.5 µm in the absorbing edge measurements. For the positron imaging of a leaf sample, the proposed method achieved lower root mean square error relative to phosphor plate imaging, and higher similarity with the reference optical image. The improvements of the preliminary results support further investigation of the proposed algorithm for the enhancement of positron imaging in clinical and preclinical applications.
[Mh] Termos MeSH primário: Fluordesoxiglucose F18/farmacocinética
Interpretação de Imagem Assistida por Computador/métodos
Folhas de Planta
Tomografia por Emissão de Pósitrons/instrumentação
Tomografia por Emissão de Pósitrons/métodos
Máquina de Vetores de Suporte
Vitaceae
[Mh] Termos MeSH secundário: Algoritmos
Simulação por Computador
Elétrons
Seres Humanos
Método de Monte Carlo
Compostos Radiofarmacêuticos/farmacocinética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Radiopharmaceuticals); 0Z5B2CJX4D (Fluorodeoxyglucose F18)
[Em] Mês de entrada:1601
[Cu] Atualização por classe:150625
[Lr] Data última revisão:
150625
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150619
[St] Status:MEDLINE
[do] DOI:10.1088/0031-9155/60/13/5261



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