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[PMID]:27815201
[Au] Autor:Pardo BG; Álvarez-Dios JA; Cao A; Ramilo A; Gómez-Tato A; Planas JV; Villalba A; Martínez P
[Ad] Endereço:Departamento de Zoología, Genética y Antropología Física, Facultad de Veterinaria, Universidade de Santiago de Compostela, Campus de Lugo, 27002 Lugo, Spain. Electronic address: belen.gomez@usc.es.
[Ti] Título:Construction of an Ostrea edulis database from genomic and expressed sequence tags (ESTs) obtained from Bonamia ostreae infected haemocytes: Development of an immune-enriched oligo-microarray.
[So] Source:Fish Shellfish Immunol;59:331-344, 2016 Dec.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The flat oyster, Ostrea edulis, is one of the main farmed oysters, not only in Europe but also in the United States and Canada. Bonamiosis due to the parasite Bonamia ostreae has been associated with high mortality episodes in this species. This parasite is an intracellular protozoan that infects haemocytes, the main cells involved in oyster defence. Due to the economical and ecological importance of flat oyster, genomic data are badly needed for genetic improvement of the species, but they are still very scarce. The objective of this study is to develop a sequence database, OedulisDB, with new genomic and transcriptomic resources, providing new data and convenient tools to improve our knowledge of the oyster's immune mechanisms. Transcriptomic and genomic sequences were obtained using 454 pyrosequencing and compiled into an O. edulis database, OedulisDB, consisting of two sets of 10,318 and 7159 unique sequences that represent the oyster's genome (WG) and de novo haemocyte transcriptome (HT), respectively. The flat oyster transcriptome was obtained from two strains (naïve and tolerant) challenged with B. ostreae, and from their corresponding non-challenged controls. Approximately 78.5% of 5619 HT unique sequences were successfully annotated by Blast search using public databases. A total of 984 sequences were identified as being related to immune response and several key immune genes were identified for the first time in flat oyster. Additionally, transcriptome information was used to design and validate the first oligo-microarray in flat oyster enriched with immune sequences from haemocytes. Our transcriptomic and genomic sequencing and subsequent annotation have largely increased the scarce resources available for this economically important species and have enabled us to develop an OedulisDB database and accompanying tools for gene expression analysis. This study represents the first attempt to characterize in depth the O. edulis haemocyte transcriptome in response to B. ostreae through massively sequencing and has aided to improve our knowledge of the immune mechanisms of flat oyster. The validated oligo-microarray and the establishment of a reference transcriptome will be useful for large-scale gene expression studies in this species.
[Mh] Termos MeSH primário: Bases de Dados Genéticas
Genoma
Haplosporídios/imunologia
Imunidade Inata
Análise de Sequência com Séries de Oligonucleotídeos
Ostrea/genética
Ostrea/parasitologia
[Mh] Termos MeSH secundário: Animais
Etiquetas de Sequências Expressas
Hemócitos/imunologia
Hemócitos/metabolismo
Hemócitos/parasitologia
Ostrea/imunologia
Análise de Sequência de DNA
Análise de Sequência de RNA
Transcriptoma
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170321
[Lr] Data última revisão:
170321
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161106
[St] Status:MEDLINE


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[PMID]:27431587
[Au] Autor:Gervais O; Chollet B; Renault T; Arzul I
[Ad] Endereço:Ifremer, RBE-SG2M-LGPMM, Station de La Tremblade, Avenue de Mus de Loup, F-17390, La Tremblade, France.
[Ti] Título:Flat oyster follows the apoptosis pathway to defend against the protozoan parasite Bonamia ostreae.
[So] Source:Fish Shellfish Immunol;56:322-329, 2016 Sep.
[Is] ISSN:1095-9947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The in vitro model Ostrea edulis hemocyte - Bonamia ostreae is interesting to investigate host-parasite interactions at the cellular level. Indeed, this unicellular parasite infects the flat oyster Ostrea edulis and multiplies within hemocytes, the central effectors of oyster defenses. Apoptosis is a mechanism used by many organisms to eliminate infected cells. In order to study the potential involvement of this mechanism in the oyster response to B. ostreae, in vitro experiments were carried out by exposing hemocytes from the naturally susceptible oyster O. edulis and a resistant oyster species Crassostrea gigas to live and heat-inactivated parasites. Hemocyte apoptotic response was measured using a combination of flow cytometry and microscopy analyses. Whatever the host species was, the parasite was engulfed in hemocytes and induced an increase of apoptotic parameters including intracytoplasmic calcium concentration, mitochondrial membrane potential or phosphatidyl-serine externalization as well as ultrastructural modifications. However, the parasite appears more able to infect flat oyster than cupped oyster hemocytes and the apoptotic response was more important against live than dead parasites in the natural host than in C. gigas. Our results suggest that O. edulis specifically responds to B. ostreae by inducing apoptosis of hemocytes.
[Mh] Termos MeSH primário: Apoptose
Haplosporídios/fisiologia
Interações Hospedeiro-Parasita
Ostrea/fisiologia
Ostrea/parasitologia
[Mh] Termos MeSH secundário: Animais
Citometria de Fluxo
Hemócitos/parasitologia
Hemócitos/fisiologia
Hemócitos/ultraestrutura
Microscopia Eletrônica de Transmissão
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1703
[Cu] Atualização por classe:170812
[Lr] Data última revisão:
170812
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160720
[St] Status:MEDLINE


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[PMID]:27263626
[Au] Autor:Pagenkopp Lohan KM; Hill-Spanik KM; Torchin ME; Aguirre-Macedo L; Fleischer RC; Ruiz GM
[Ad] Endereço:Marine Invasions Laboratory,Smithsonian Environmental Research Center,Edgewater,Maryland 21037,USA.
[Ti] Título:Richness and distribution of tropical oyster parasites in two oceans.
[So] Source:Parasitology;143(9):1119-32, 2016 08.
[Is] ISSN:1469-8161
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Parasites can exert strong effects on population to ecosystem level processes, but data on parasites are limited for many global regions, especially tropical marine systems. Characterizing parasite diversity and distributions are the first steps towards understanding the potential impacts of parasites. The Panama Canal serves as an interesting location to examine tropical parasite diversity and distribution, as it is a conduit between two oceans and a hub for international trade. We examined metazoan and protistan parasites associated with ten oyster species collected from both Panamanian coasts, including the Panama Canal and Bocas del Toro. We found multiple metazoan taxa (pea crabs, Stylochus spp., Urastoma cyrinae). Our molecular screening for protistan parasites detected four species of Perkinsus (Perkinsus marinus, Perkinsus chesapeaki, Perkinsus olseni, Perkinsus beihaiensis) and several haplosporidians, including two genera (Minchinia, Haplosporidium). Species richness was higher for the protistan parasites than for the metazoans, with haplosporidian richness being higher than Perkinsus richness. Perkinsus species were the most frequently detected and most geographically widespread among parasite groups. Parasite richness and overlap differed between regions, locations and oyster hosts. These results have important implications for tropical parasite richness and the dispersal of parasites due to shipping associated with the Panama Canal.
[Mh] Termos MeSH primário: Haplosporídios/classificação
Ostreidae/parasitologia
Platelmintos/classificação
[Mh] Termos MeSH secundário: Animais
Teorema de Bayes
Região do Caribe
DNA de Protozoário/química
DNA de Protozoário/isolamento & purificação
Haplosporídios/genética
Haplosporídios/isolamento & purificação
Funções Verossimilhança
Ostreidae/classificação
Oceano Pacífico
Panamá
Zona do Canal do Panamá
Filogenia
Platelmintos/genética
Platelmintos/isolamento & purificação
Salinidade
Estações do Ano
Clima Tropical
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Protozoan)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171002
[Lr] Data última revisão:
171002
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160607
[St] Status:MEDLINE
[do] DOI:10.1017/S0031182015001900


  4 / 105 MEDLINE  
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[PMID]:26880159
[Au] Autor:Flannery G; Lynch SA; Culloty SC
[Ad] Endereço:Aquaculture and Fisheries Development Centre, School of Biological, Earth and Environmental Sciences, University College Cork, Ireland. Electronic address: grace.flannery@umail.ucc.ie.
[Ti] Título:Investigating the significance of the role of Ostrea edulis larvae in the transmission and transfer of Bonamia ostreae.
[So] Source:J Invertebr Pathol;136:7-9, 2016 May.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, the ability of oyster larvae, brooded in the pallial cavity of the parent oyster, to become infected in the pallial fluid, which is influenced by the brooding oyster and surrounding environment, was investigated. Larvae were collected over three summers from three areas around Ireland. Samples were screened for the presence of Bonamia ostreae DNA using PCR analysis. Four samples of larvae were found to be positive for B. ostreae DNA, though the parent oysters were negative for infection. Larvae may be able to acquire the pathogen from the water column during filter feeding or elimination of pseudo-faeces by the brooding adult.
[Mh] Termos MeSH primário: Larva/microbiologia
Ostrea/microbiologia
Infecções por Protozoários/transmissão
[Mh] Termos MeSH secundário: Animais
Haplosporídios
Interações Hospedeiro-Parasita/fisiologia
Irlanda
Reação em Cadeia da Polimerase
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170926
[Lr] Data última revisão:
170926
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160217
[St] Status:MEDLINE


  5 / 105 MEDLINE  
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[PMID]:26865235
[Au] Autor:Lane HS; Webb SC; Duncan J
[Ad] Endereço:Department of Zoology, University of Otago, PO Box 56, Dunedin 9054, New Zealand.
[Ti] Título:Bonamia ostreae in the New Zealand oyster Ostrea chilensis: a new host and geographic record for this haplosporidian parasite.
[So] Source:Dis Aquat Organ;118(1):55-63, 2016 Feb 11.
[Is] ISSN:0177-5103
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Previous reports of the haplosporidian parasite Bonamia ostreae have been restricted to the Northern Hemisphere, including Europe, and both eastern and western North America. This species is reported for the first time in New Zealand infecting the flat oyster Ostrea chilensis. Histological examination of 149 adult oysters identified 119 (79.9%) infected with Bonamia microcells. Bonamia generic PCR of several oysters followed by DNA sequencing of a 300 bp portion of the 18S rDNA gene produced a 100% match with that of B. ostreae. All DNA-sequenced products also produced a B. ostreae PCR-restriction fragment length polymorphism (PCR-RFLP) profile. Bonamia species-specific PCRs further detected single infections of B. exitiosa (2.7%), B. ostreae (40.3%), and concurrent infections (53.7%) with these 2 Bonamia species identifying overall a Bonamia prevalence of 96.6%. Detailed histological inspection revealed 2 microcell types. An infection identified by PCR as B. ostreae histologically presented small microcells (mean ± SE diameter = 1.28 ± 0.16 µm, range = 0.9-2 µm, n = 60) commonly with eccentric nuclei. A B. exitiosa infection exhibited larger microcells (mean ± SE diameter = 2.12 ± 0.27 µm, range = 1.5-4 µm, n = 60) with more concentric nuclei. Concurrent infections of both Bonamia species, as identified by PCR, exhibited both types of microcells. DNA barcoding of the B. ostreae-infected oyster host confirmed the identification as O. chilensis. A suite of other parasites that accompany O. chilensis are reported here for the first time in mixed infection with B. ostreae including apicomplexan X (76.5%), Microsporidium rapuae (0.7%) and Bucephalus longicornutus (30.2%).
[Mh] Termos MeSH primário: Haplosporídios/fisiologia
Ostrea/parasitologia
[Mh] Termos MeSH secundário: Animais
Interações Hospedeiro-Parasita
Nova Zelândia
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1605
[Cu] Atualização por classe:160211
[Lr] Data última revisão:
160211
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160212
[St] Status:MEDLINE
[do] DOI:10.3354/dao02960


  6 / 105 MEDLINE  
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[PMID]:26096446
[Au] Autor:Batista FM; López-Sanmartín M; Grade A; Navas JI; Ruano F
[Ad] Endereço:Divisão de Aquicultura e Valorização, Instituto Português do Mar e da Atmosfera (IPMA), Lisboa, Portugal.
[Ti] Título:Detection of Bonamia exitiosa in the European flat oyster Ostrea edulis in southern Portugal.
[So] Source:J Fish Dis;39(5):607-11, 2016 May.
[Is] ISSN:1365-2761
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Haplosporídios/fisiologia
Ostrea/parasitologia
[Mh] Termos MeSH secundário: Animais
Aquicultura
Haplosporídios/genética
Reação em Cadeia da Polimerase
Portugal
Proteínas de Protozoários/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Protozoan Proteins)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150623
[St] Status:MEDLINE
[do] DOI:10.1111/jfd.12396


  7 / 105 MEDLINE  
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[PMID]:26282916
[Au] Autor:Prado-Alvarez M; Couraleau Y; Chollet B; Tourbiez D; Arzul I
[Ad] Endereço:Laboratoire de Génétique et Pathologie des Mollusques Marins,IFREMER,Avenue de Mus de Loup,17390 La Tremblade,France.
[Ti] Título:Whole-genome amplification: a useful approach to characterize new genes in unculturable protozoan parasites such as Bonamia exitiosa.
[So] Source:Parasitology;142(12):1523-34, 2015 Oct.
[Is] ISSN:1469-8161
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bonamia exitiosa is an intracellular parasite (Haplosporidia) that has been associated with mass mortalities in oyster populations in the Southern hemisphere. This parasite was recently detected in the Northern hemisphere including Europe. Some representatives of the Bonamia genus have not been well categorized yet due to the lack of genomic information. In the present work, we have applied Whole-Genome Amplification (WGA) technique in order to characterize the actin gene in the unculturable protozoan B. exitiosa. This is the first protein coding gene described in this species. Molecular analysis revealed that B. exitiosa actin is more similar to Bonamia ostreae actin gene-1. Actin phylogeny placed the Bonamia sp. infected oysters in the same clade where the herein described B. exitiosa actin resolved, offering novel information about the classification of the genus. Our results showed that WGA methodology is a promising and valuable technique to be applied to unculturable protozoans whose genomic material is limited.
[Mh] Termos MeSH primário: Genoma de Protozoário/genética
Haplosporídios/classificação
Ostreidae/parasitologia
Reação em Cadeia da Polimerase/veterinária
[Mh] Termos MeSH secundário: Actinas/genética
Sequência de Aminoácidos
Animais
Sequência de Bases
DNA de Protozoário/química
DNA de Protozoário/genética
Europa (Continente)
Haplosporídios/genética
Haplosporídios/isolamento & purificação
Dados de Sequência Molecular
Filogenia
Alinhamento de Sequência/veterinária
Análise de Sequência de DNA/veterinária
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Actins); 0 (DNA, Protozoan)
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150819
[St] Status:MEDLINE
[do] DOI:10.1017/S0031182015000967


  8 / 105 MEDLINE  
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[PMID]:26271576
[Au] Autor:Le TC; Kang HS; Hong HK; Park KJ; Choi KS
[Ad] Endereço:School of Marine Biomedical Science (BK21 PLUS), Jeju National University, 102 Jejudaehakno, Jeju 690-756, Republic of Korea.
[Ti] Título:First report of Urosporidium sp., a haplosporidian hyperparasite infecting digenean trematode Parvatrema duboisi in Manila clam, Ruditapes philippinarum on the west coast of Korea.
[So] Source:J Invertebr Pathol;130:141-6, 2015 Sep.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:In this study, we first report on the occurrence of Urosporidium sp., a haplosporidian hyperparasite infecting the trematode, Parvatrema duboisi, which parasitizes Manila clams, Ruditapes philippinarum on the west and south coasts of Korea. The larval P. duboisi infected by the sporocyst stage of Urosporidium sp. demonstrated numerous small yellowish spores in their tissues. The heavily infected metacercariae exhibited degenerate bodies and the larvae were often motionless. Clams heavily infected by the metacercariae of P. duboisi also displayed abnormal golden spots on the mantle tissue. In histology, different life stages of Urosporidium sp. could be identified, including the uni-nucleate, plasmodial, sporogonic stages and the acid fast mature spores released from the cyst. In scanning electron microscopy (SEM), the mature spore exhibited a semi-circular rim around the apical end and the orifice was covered internally with a flap. Loop-like filaments ornamentation was also identified from Urosporidium sp. in SEM, suggesting that Urosporidium sp. found in this study is a new member in the genus. Prevalence of Urosporidium sp.-infected trematodes in this study ranged from 2.5% to 24.0% in April 2010 and the infection was observed from 8 sampling sites out of the 26 sites surveyed on the west and south coasts.
[Mh] Termos MeSH primário: Bivalves/parasitologia
Haplosporídios/parasitologia
Trematódeos
[Mh] Termos MeSH secundário: Animais
Microscopia Eletrônica de Varredura
Prevalência
República da Coreia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150815
[St] Status:MEDLINE


  9 / 105 MEDLINE  
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[PMID]:26264670
[Au] Autor:Arzul I; Carnegie RB
[Ad] Endereço:IFREMER, Laboratory of Genetics and Pathology, Av de Mus de Loup, 17390 La Tremblade, France.
[Ti] Título:New perspective on the haplosporidian parasites of molluscs.
[So] Source:J Invertebr Pathol;131:32-42, 2015 Oct.
[Is] ISSN:1096-0805
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The protist phylum Haplosporidia comprises over 40 described species with representatives infecting a range of mollusc hosts, including several ecologically and economically significant pathogens. Continuing exploration of haplosporidian diversity has added ten new species in recent years and brought the phylogenetics of the group into somewhat clearer focus, with monophyletic Bonamia and Minchinia lineages continuing to be supported. However, the addition of new sequences to phylogenetic analyses has left the paraphyletic genus Haplosporidium's picture less resolved. It is not clear that even two genera will be enough to accommodate the species presently drawn to the Haplosporidium regions of the haplosporidian tree. In this review, we summarize recent findings in haplosporidian diversity and phylogenetics, and provide a synthesis of our understanding of the life cycles and environmental influences on haplosporidians, with particular emphasis on the important pathogens Haplosporidium nelsoni and Bonamia ostreae. Additionally, we consider the evolution of the "microcell haplosporidian" lifestyle of Bonamia parasites, and suggest that colonization of high-density oyster host populations in relatively stable euhaline marine environments may have been an important development favoring the evolution of the microcell haplosporidian life strategy.
[Mh] Termos MeSH primário: Haplosporídios
Ostreidae/parasitologia
[Mh] Termos MeSH secundário: Animais
Interações Hospedeiro-Parasita
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1611
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150813
[St] Status:MEDLINE


  10 / 105 MEDLINE  
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[PMID]:26179094
[Au] Autor:Gagné N; Veniot A; Stephenson M; McClure C
[Ad] Endereço:Fisheries and Oceans Canada, Gulf Fisheries Centre, Moncton, New Brunswick, Canada (Gagné, Veniot, Stephenson)AquaEpi Research, Charlottetown, Prince Edward Island, Canada (McClure) nellie.gagne@dfo-mpo.gc.ca.
[Ti] Título:Performance characteristics of polymerase chain reaction and histological methods for the detection of Haplosporidium nelsoni in the eastern oyster (Crassostrea virginica).
[So] Source:J Vet Diagn Invest;27(4):476-88, 2015 Jul.
[Is] ISSN:1943-4936
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Fitness for purpose and validation are increasingly becoming a benchmark in the development of test methods for the diagnosis of infectious diseases in aquatic animals. The design of the evaluation and the analysis of data are critical to demonstrate test method performance characteristics and fitness for purpose, as stated in the World Organization for Animal Health pathway for test validation. Three test methods for the detection of the oyster parasite Haplosporidium nelsoni were selected for the validation study described herein: histology, end-point polymerase chain reaction (PCR), and real-time PCR (qPCR). Preliminary work evaluated the analytical sensitivity and specificity of the PCR and qPCR assay in development. The following stage used test results on 100 oysters in 3 different laboratories to assess diagnostic sensitivity (DSe), diagnostic specificity (DSp), repeatability, and reproducibility. Repeatability and reproducibility were within 68-95%. The final part of the project evaluated DSe and DSp using test results on 400 oysters and results from the first 100 oysters tested. In the absence of a 100% gold standard test, latent class modeling methods were explored to characterize the tests (i.e., Bayesian analyses). For both PCR methods, DSe was >90%, and in the 60% range for histology, whereas DSp was >90% for all methods. Based on the results of this validation, a threshold cycle value of 30 for qPCR corresponds to the limit of sensitivity for histology where unreliable detection becomes more frequent, thus providing a threshold helpful in diagnostic settings where both histology and qPCR are used.
[Mh] Termos MeSH primário: Crassostrea/parasitologia
Haplosporídios/isolamento & purificação
Infecções por Protozoários/parasitologia
[Mh] Termos MeSH secundário: Animais
Teorema de Bayes
Canadá
Haplosporídios/genética
Reação em Cadeia da Polimerase em Tempo Real/veterinária
Reprodutibilidade dos Testes
Sensibilidade e Especificidade
[Pt] Tipo de publicação:EVALUATION STUDIES; JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150803
[Lr] Data última revisão:
150803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150717
[St] Status:MEDLINE
[do] DOI:10.1177/1040638715592666



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