Base de dados : MEDLINE
Pesquisa : B01.750 [Categoria DeCS]
Referências encontradas : 502 [refinar]
Mostrando: 1 .. 10   no formato [Detalhado]

página 1 de 51 ir para página                         

  1 / 502 MEDLINE  
              next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29367487
[Au] Autor:Lin Y; Xie X; Yuan B; Fu J; Liu L; Tian H; Chen T; He D
[Ad] Endereço:College of Food Science and Engineering, Wuhan polytechnic University.
[Ti] Título:Optimization of Enzymatic Cell Disruption for Improving Lipid Extraction from Schizochytrium sp. through Response Surface Methodology.
[So] Source:J Oleo Sci;67(2):215-224, 2018 Feb 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:This study is aimed to explore the optimal conditions of cell disruption in the extraction algae oil process, using alkaline protease to disrupt cell of Schizochytrium sp. to extract oil in this paper. The effects of enzymatic lysis temperature, enzymatic lysis time, enzyme dosage and pH value on oil yield and DHA yield were studied. Through the combination of single factor test and response surface design, the optimal cell disruption conditions were screened out. The fatty acid composition of algal oil was analyzed by gas chromatography-massspectrometry (GC-MS). The results showed that when the conditions were: enzymatic lysis temperature 55°C, enzymatic lysis time 9 h, enzyme dosage 3% of biomass and pH 8,oil yield and DHA yield reached the highest 14.52 g/L and 7.12 g/L, respectively. When the strains were cultured in 50 L fermentor, oil yield reached 26.27 g/L and DHA yield reached 12.89 g/L. They were 1.81 times higher than that in shake-flask cultivation. The optimization experiment provides the basis for the industrial production of Schizochytrium sp.
[Mh] Termos MeSH primário: Proteínas de Bactérias
Endopeptidases
Extração Líquido-Líquido/métodos
Óleos/isolamento & purificação
Estramenópilas/química
[Mh] Termos MeSH secundário: Ácidos Graxos/análise
Cromatografia Gasosa-Espectrometria de Massas
Concentração de Íons de Hidrogênio
Óleos/química
Estramenópilas/citologia
Temperatura Ambiente
Fatores de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Fatty Acids); 0 (Oils); EC 3.4.- (Endopeptidases); EC 3.4.99.- (alkaline protease)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180126
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17166


  2 / 502 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29202688
[Au] Autor:Abrahamian M; Kagda M; Ah-Fong AMV; Judelson HS
[Ad] Endereço:Department of Microbiology and Plant Pathology, University of California, Riverside, CA, 92521, USA.
[Ti] Título:Rethinking the evolution of eukaryotic metabolism: novel cellular partitioning of enzymes in stramenopiles links serine biosynthesis to glycolysis in mitochondria.
[So] Source:BMC Evol Biol;17(1):241, 2017 Dec 04.
[Is] ISSN:1471-2148
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: An important feature of eukaryotic evolution is metabolic compartmentalization, in which certain pathways are restricted to the cytosol or specific organelles. Glycolysis in eukaryotes is described as a cytosolic process. The universality of this canon has been challenged by recent genome data that suggest that some glycolytic enzymes made by stramenopiles bear mitochondrial targeting peptides. RESULTS: Mining of oomycete, diatom, and brown algal genomes indicates that stramenopiles encode two forms of enzymes for the second half of glycolysis, one with and the other without mitochondrial targeting peptides. The predicted mitochondrial targeting was confirmed by using fluorescent tags to localize phosphoglycerate kinase, phosphoglycerate mutase, and pyruvate kinase in Phytophthora infestans, the oomycete that causes potato blight. A genome-wide search for other enzymes with atypical mitochondrial locations identified phosphoglycerate dehydrogenase, phosphoserine aminotransferase, and phosphoserine phosphatase, which form a pathway for generating serine from the glycolytic intermediate 3-phosphoglycerate. Fluorescent tags confirmed the delivery of these serine biosynthetic enzymes to P. infestans mitochondria. A cytosolic form of this serine biosynthetic pathway, which occurs in most eukaryotes, is missing from oomycetes and most other stramenopiles. The glycolysis and serine metabolism pathways of oomycetes appear to be mosaics of enzymes with different ancestries. While some of the noncanonical oomycete mitochondrial enzymes have the closest affinity in phylogenetic analyses with proteins from other stramenopiles, others cluster with bacterial, plant, or animal proteins. The genes encoding the mitochondrial phosphoglycerate kinase and serine-forming enzymes are physically linked on oomycete chromosomes, which suggests a shared origin. CONCLUSIONS: Stramenopile metabolism appears to have been shaped through the acquisition of genes by descent and lateral or endosymbiotic gene transfer, along with the targeting of the proteins to locations that are novel compared to other eukaryotes. Colocalization of the glycolytic and serine biosynthesis enzymes in mitochondria is apparently necessary since they share a common intermediate. The results indicate that descriptions of metabolism in textbooks do not cover the full diversity of eukaryotic biology.
[Mh] Termos MeSH primário: Evolução Biológica
Células Eucarióticas/metabolismo
Glicólise
Mitocôndrias/metabolismo
Serina/biossíntese
Estramenópilas/enzimologia
Estramenópilas/metabolismo
[Mh] Termos MeSH secundário: Animais
Citosol
Genes
Mitocôndrias/genética
Oomicetos/metabolismo
Fosforilação
Filogenia
Phytophthora infestans/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
452VLY9402 (Serine)
[Em] Mês de entrada:1803
[Cu] Atualização por classe:180309
[Lr] Data última revisão:
180309
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171206
[St] Status:MEDLINE
[do] DOI:10.1186/s12862-017-1087-8


  3 / 502 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:29191192
[Au] Autor:Suh SS; Kim SM; Kim JE; Hong JM; Lee SG; Youn UJ; Han SJ; Kim IC; Kim S
[Ad] Endereço:Division of Polar Life Sciences, Korea Polar Research Institute, Incheon, 21990, Republic of Korea.
[Ti] Título:Anticancer activities of ethanol extract from the Antarctic freshwater microalga, Botryidiopsidaceae sp.
[So] Source:BMC Complement Altern Med;17(1):509, 2017 Dec 01.
[Is] ISSN:1472-6882
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Cancer is a leading cause of human death around the world and occurs through the highly complex coordination of multiple cellular pathways. Recent studies have revealed that microalgal extracts exhibit considerable pharmaceutical activities, including those against various cancer cells. Thus, microalgae are promising candidates as novel cancer therapeutic drugs. In this study, we evaluated the biological functions of the ethanolic extract of the Antarctic freshwater microalga, Bo tryidiopsidaceae sp., such as its antioxidant, anti-proliferative, apoptotic and anti-invasive properties. METHODS: To estimate antioxidant capacity of ethanol extract of Bo tryidiopsidaceae sp. (ETBO), free radical 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and 1,1-diphenyl-2-picrylhydrazyl (DPPH) assays were used. The anti-proliferative activity of ETBO was assessed in several cancer cell lines (A375, Hs578T and HeLa) and non-tumorigenic keratinocyte cells (HaCaT), using MTT assay. In addition, Annexin V binding was performed to detect ETBO-induced apoptotic cells, and the expression levels of apoptosis-regulating proteins, caspase-3, p53, and Bcl-2, were determined by western blot. Boyden chamber assays were used to determine anti-migratory and anti-invasive properties of ETBO. RESULTS: ETBO exhibited antioxidant activity and concentration-dependent anticancer activities, such as anti-proliferation and pro-apoptotic activities against cancer cells. Furthermore, the expression of the apoptosis-inducing proteins, p53 and caspase-3, significantly increased in response to ETBO, whereas the expression of the anti-apoptotic protein, Bcl-2, decreased. These data imply that ETBO induces apoptosis by caspase activation through the modulation of pro-apoptotic and anti-apoptotic gene, p53 and Bcl-2, respectively. In addition, ETBO significantly inhibited migration and invasion of cervical cancer cells in a concentration-dependent manner. CONCLUSION: In this study, ETBO exhibited considerable anticancer activities, such as inhibition of proliferation, invasion, and migration, as well as induction of apoptosis. These data suggest that ETBO is a promising therapeutic agent in cancer therapy and drug discovery.
[Mh] Termos MeSH primário: Antineoplásicos/farmacologia
Microalgas/química
Extratos Vegetais/farmacologia
Estramenópilas/química
[Mh] Termos MeSH secundário: Regiões Antárticas
Antineoplásicos/química
Apoptose/efeitos dos fármacos
Linhagem Celular Tumoral
Movimento Celular/efeitos dos fármacos
Proliferação Celular/efeitos dos fármacos
Água Doce
Células HeLa
Seres Humanos
Extratos Vegetais/química
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Antineoplastic Agents); 0 (Plant Extracts)
[Em] Mês de entrada:1712
[Cu] Atualização por classe:171220
[Lr] Data última revisão:
171220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:171202
[St] Status:MEDLINE
[do] DOI:10.1186/s12906-017-1991-x


  4 / 502 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28795620
[Au] Autor:Otagiri M; Khalid A; Moriya S; Osada H; Takahashi S
[Ad] Endereço:a Biomass Research Platform Team , RIKEN Centre for Sustainable Resource Science , Yokohama , Japan.
[Ti] Título:Novel squalene-producing thraustochytrids found in mangrove water.
[So] Source:Biosci Biotechnol Biochem;81(10):2034-2037, 2017 Oct.
[Is] ISSN:1347-6947
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:On extended screening of squalene-producing strains in Okinawa mangrove water, we identified 14 novel squalene-producing thraustochytrids from 172 unialgal clonal isolates. The novel thraustochytrids produced 13.9-7.54 mg squalene/g dry cell weight. Eight isolates were found to belong to potentially novel squalene-producing genera, forming a monophyletic cluster independent from any known thraustochytrids.
[Mh] Termos MeSH primário: Esqualeno/metabolismo
Estramenópilas/metabolismo
Microbiologia da Água
Zonas Úmidas
[Mh] Termos MeSH secundário: RNA Ribossômico 18S/genética
Estramenópilas/genética
Estramenópilas/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 18S); 7QWM220FJH (Squalene)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171017
[Lr] Data última revisão:
171017
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170811
[St] Status:MEDLINE
[do] DOI:10.1080/09168451.2017.1359485


  5 / 502 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28701042
[Au] Autor:Teuling E; Wierenga PA; Schrama JW; Gruppen H
[Ad] Endereço:Aquaculture and Fisheries Group and ‡Laboratory of Food Chemistry, Wageningen University , Wageningen, The Netherlands.
[Ti] Título:Comparison of Protein Extracts from Various Unicellular Green Sources.
[So] Source:J Agric Food Chem;65(36):7989-8002, 2017 Sep 13.
[Is] ISSN:1520-5118
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Photosynthetic unicellular organisms are considered as promising alternative protein sources. The aim of this study is to understand the extent to which these green sources differ with respect to their gross composition and how these differences affect the final protein isolate. Using mild isolation techniques, proteins were extracted and isolated from four different unicellular sources (Arthrospira (spirulina) maxima, Nannochloropsis gaditana, Tetraselmis impellucida, and Scenedesmus dimorphus). Despite differences in protein contents of the sources (27-62% w/w) and in protein extractability (17-74% w/w), final protein isolates were obtained that had similar protein contents (62-77% w/w) and protein yields (3-9% w/w). Protein solubility as a function of pH was different between the sources and in ionic strength dependency, especially at pH < 4.0. Overall, the characterization and extraction protocol used allows a relatively fast and well-described isolation of purified proteins from novel protein sources.
[Mh] Termos MeSH primário: Clorófitas/química
Proteínas/química
Scenedesmus/química
Spirulina/química
Estramenópilas/química
[Mh] Termos MeSH secundário: Concentração de Íons de Hidrogênio
Concentração Osmolar
Proteínas/isolamento & purificação
Solubilidade
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Proteins)
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170925
[Lr] Data última revisão:
170925
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170714
[St] Status:MEDLINE
[do] DOI:10.1021/acs.jafc.7b01788


  6 / 502 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28628130
[Au] Autor:Ajjawi I; Verruto J; Aqui M; Soriaga LB; Coppersmith J; Kwok K; Peach L; Orchard E; Kalb R; Xu W; Carlson TJ; Francis K; Konigsfeld K; Bartalis J; Schultz A; Lambert W; Schwartz AS; Brown R; Moellering ER
[Ad] Endereço:Synthetic Genomics Inc., La Jolla, California, USA.
[Ti] Título:Lipid production in Nannochloropsis gaditana is doubled by decreasing expression of a single transcriptional regulator.
[So] Source:Nat Biotechnol;35(7):647-652, 2017 Jul.
[Is] ISSN:1546-1696
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Lipid production in the industrial microalga Nannochloropsis gaditana exceeds that of model algal species and can be maximized by nutrient starvation in batch culture. However, starvation halts growth, thereby decreasing productivity. Efforts to engineer N. gaditana strains that can accumulate biomass and overproduce lipids have previously met with little success. We identified 20 transcription factors as putative negative regulators of lipid production by using RNA-seq analysis of N. gaditana during nitrogen deprivation. Application of a CRISPR-Cas9 reverse-genetics pipeline enabled insertional mutagenesis of 18 of these 20 transcription factors. Knocking out a homolog of fungal Zn(II) Cys -encoding genes improved partitioning of total carbon to lipids from 20% (wild type) to 40-55% (mutant) in nutrient-replete conditions. Knockout mutants grew poorly, but attenuation of Zn(II) Cys expression yielded strains producing twice as much lipid (∼5.0 g m d ) as that in the wild type (∼2.5 g m d ) under semicontinuous growth conditions and had little effect on growth.
[Mh] Termos MeSH primário: Melhoramento Genético/métodos
Metabolismo dos Lipídeos/genética
Lipídeos/biossíntese
Elementos Reguladores de Transcrição/genética
Estramenópilas/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Proteínas de Algas/genética
Regulação para Baixo/genética
Técnicas de Inativação de Genes
Lipídeos/genética
Estramenópilas/crescimento & desenvolvimento
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Algal Proteins); 0 (Lipids); 0 (Transcription Factors)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170818
[Lr] Data última revisão:
170818
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1038/nbt.3865


  7 / 502 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28626144
[Au] Autor:Adachi T; Sahara T; Okuyama H; Morita N
[Ad] Endereço:Laboratory of Molecular Environmental Microbiology, Graduate School of Agriculture, Hokkaido University.
[Ti] Título:Glass Bead-based Genetic Transformation:An Efficient Method for Transformation of Thraustochytrid Microorganisms.
[So] Source:J Oleo Sci;66(7):791-795, 2017 Jul 01.
[Is] ISSN:1347-3352
[Cp] País de publicação:Japan
[La] Idioma:eng
[Ab] Resumo:Here, we describe a new method for genetic transformation of thraustochytrids, well-known producers of polyunsaturated fatty acids (PUFAs) like docosahexaenoic acid, by combining mild glass (zirconia) bead treatment and electroporation. Because the cell wall is a barrier against transfer of exogenous DNA into cells, gentle vortexing of cells with glass beads was performed prior to electroporation for partial cell wall disruption. G418-resistant transformants of thraustochytrid cells (Aurantiochytrium limacinum strain SR21 and thraustochytrid strain 12B) were successfully obtained with good reproducibility. The method reported here is simpler than methods using enzymes to generate spheroplasts and may provide advantages for PUFA production by using genetically modified thraustochytrids.
[Mh] Termos MeSH primário: Vidro
Estramenópilas/genética
Transformação Genética
Zircônio
[Mh] Termos MeSH secundário: Parede Celular
DNA
Eletroporação
Ácidos Graxos Insaturados/biossíntese
Reprodutibilidade dos Testes
Estramenópilas/citologia
Estramenópilas/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids, Unsaturated); 9007-49-2 (DNA); C6V6S92N3C (Zirconium); S38N85C5G0 (zirconium oxide)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171010
[Lr] Data última revisão:
171010
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.5650/jos.ess17084


  8 / 502 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28531849
[Au] Autor:Zhou W; Wang H; Chen L; Cheng W; Liu T
[Ad] Endereço:Key Laboratory of Biofuels, Key Laboratory of Shandong Energy Biological Genetic Resources, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao 266071, China.
[Ti] Título:Heterotrophy of filamentous oleaginous microalgae Tribonema minus for potential production of lipid and palmitoleic acid.
[So] Source:Bioresour Technol;239:250-257, 2017 Sep.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Heterotrophic fermentation and high valuable co-product producing are thought to be effective ways to improve the economic viability and feasibility of commercial production of microalgae biofuels. This work reported the heterotrophic cultivation of Tribonema minus for lipid and palmitoleic acid (a novel functional fatty acid) production. Firstly, the heterotrophic ability of T. minus was identified for the first time with significant promotion in biomass and lipid productivity, and glucose and urea were then selected as the optimal carbon and nitrogen sources. Moreover, nutrient concentrations and culture conditions were optimized. Highest biomass and lipid productivity of 30.8gL and 730mgL d were obtained respectively by adding 80gL glucose at once. In addition, 2gL urea, 0.8gL K HPO , 24mgL ammonium ferric citrate, initial pH of 6, and temperature of 27°C were determined as the appropriate conditions for heterotrophic growth and lipid production.
[Mh] Termos MeSH primário: Biocombustíveis
Ácidos Graxos Monoinsaturados
Estramenópilas
[Mh] Termos MeSH secundário: Biomassa
Processos Heterotróficos
Lipídeos
Microalgas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 0 (Fatty Acids, Monounsaturated); 0 (Lipids); 209B6YPZ4I (palmitoleic acid)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170523
[St] Status:MEDLINE


  9 / 502 MEDLINE  
              first record previous record next record last record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28526282
[Au] Autor:Cheng T; Zhang W; Zhang W; Yuan G; Wang H; Liu T
[Ad] Endereço:Key Laboratory of Biofuels, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
[Ti] Título:An oleaginous filamentous microalgae Tribonema minus exhibits high removing potential of industrial phenol contaminants.
[So] Source:Bioresour Technol;238:749-754, 2017 Aug.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Discharge of industrial phenol contaminants could cause great harm on natural environment. Through oleaginous microalgae cultivation in phenolic wastewater, pollutants can be phototrophically biofixed into biomass as feedstock for bioenergy production. It was firstly reported in this study that, an oleaginous filamentous microalgae Tribonema minus exhibited strong environmental phenol removal ability. T. minus filaments showed 449.46mgg of phenol-uptake capacity, obviously higher than those strains with low phenol absorption such as Scenedesmus dimorphus. And phenols could be removed efficiently at the initial phenol concentration up to 700mgL . Simultaneously, through T. minus growth, phenol concentration could be decreased from 100mgL to the range of 0.1-0.5mgL , which meet industrial discharge need of phenol contaminants in most countries. So Tribonema minus is a potential algal specie to help the construction of integrated process for both oleaginous biomass production and bioremediation of phenol contaminants.
[Mh] Termos MeSH primário: Microalgas
Fenol
[Mh] Termos MeSH secundário: Biodegradação Ambiental
Biocombustíveis
Biomassa
Fenóis
Estramenópilas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Biofuels); 0 (Phenols); 339NCG44TV (Phenol)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170521
[St] Status:MEDLINE


  10 / 502 MEDLINE  
              first record previous record
seleciona
para imprimir
Fotocópia
Texto completo
[PMID]:28521230
[Au] Autor:Safi C; Cabas Rodriguez L; Mulder WJ; Engelen-Smit N; Spekking W; van den Broek LAM; Olivieri G; Sijtsma L
[Ad] Endereço:Wageningen Food & Biobased Research, AlgaePARC, PO Box 17, 6700 AA Wageningen, The Netherlands. Electronic address: carl.safi@wur.nl.
[Ti] Título:Energy consumption and water-soluble protein release by cell wall disruption of Nannochloropsis gaditana.
[So] Source:Bioresour Technol;239:204-210, 2017 Sep.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Several cell disruption methods were tested on Nannochloropsis gaditana, to evaluate their efficiency in terms of cell disintegration, energy input and release of soluble proteins. High-pressure homogenization (HPH) and bead milling were the most efficient with >95% cell disintegration, ±50% (w/w) release of total proteins and low energy input (<0.5kWh.kg ). Enzymatic treatment required low energy input (<0.34kWh.kg ), but it only released ±35% protein (w/w). Pulsed Electric Field (PEF) was neither energy-efficient (10.44kWh.kg ) nor successful for protein release (only 10% proteins w/w) and cell disintegration. The release of proteins after applying HPH and bead milling always required less intensive operating conditions for cell disruption. The energy cost per unit of released protein ranged from 0.15-0.25 €.kg in case of HPH, and up to 2-20 €.kg in case of PEF.
[Mh] Termos MeSH primário: Proteínas de Plantas
Estramenópilas
[Mh] Termos MeSH secundário: Biomassa
Parede Celular
Microalgas
Água
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Plant Proteins); 059QF0KO0R (Water)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171106
[Lr] Data última revisão:
171106
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170519
[St] Status:MEDLINE



página 1 de 51 ir para página                         
   


Refinar a pesquisa
  Base de dados : MEDLINE Formulário avançado   

    Pesquisar no campo  
1  
2
3
 
           



Search engine: iAH v2.6 powered by WWWISIS

BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde