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  1 / 1852 MEDLINE  
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[PMID]:29388536
[Au] Autor:Zhao B; Hu Q; Guo X; Liao Z; Sarmiento F; Mesbah NM; Yan Y; Li J; Wiegel J
[Ad] Endereço:1​Graduate School, Chinese Academy of Agricultural Sciences, Beijing 100081, PR China.
[Ti] Título:Natronolimnobius aegyptiacus sp. nov., an extremely halophilic alkalithermophilic archaeon isolated from the athalassohaline Wadi An Natrun, Egypt.
[So] Source:Int J Syst Evol Microbiol;68(2):498-506, 2018 Feb.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:An obligately aerobic extremely halophilic alkalithermophilic archaeon, strain JW/NM-HA 15 , was isolated from the sediments of Wadi An Natrun in Egypt. Phylogenetic analysis based on 16S rRNA and rpoB' gene sequences indicated that it belongs to the family Natrialbaceae of the order Natrialbales. The closest relatives were Natronolimnobius baerhuensis IHC-005 and Natronolimnobius innermongolicus N-1311 (95.3 and 94.5 % 16S rRNA gene sequence similarity, respectively). Genome relatedness between strain JW/NM-HA 15 and its neighbours was evaluated using average nucleotide identity, digital DNA-DNA hybridization and average amino acid identity with the values of 75.7-85.0, 18.1-20.0, and 70.2-71.0%, respectively. Cells were obligately aerobic, rod-shaped, non-motile, Gram-stain-negative and chemo-organotrophic. The strain grew in the presence of 2.57 M to saturating Na (optimum 3.25-4.60 M Na ), at pH 7.5-10.5 (optimum pH 9.0-9.5), and at 30-56 °C (optimum 52 °C). The major polar lipids consisted of phosphatidylglycerol, methylated phosphatidylglycerolphosphate and two phospholipids. The complete genome size of strain JW/NM-HA 15 is approximately 3.93 Mb, with a DNA G+C content of 64.1 mol%. On the basis of phylogenetic features, genomic relatedness, phenotypic and chemotaxonomic data, strain JW/NM-HA 15 was thus considered to represent a novel species within the genus Natronolimnobius, for which the name Natronolimnobius aegyptiacus sp. nov. is proposed. The type strain is JW/NM-HA 15 (=ATCC BAA-2088 =DSM 23470 ).
[Mh] Termos MeSH primário: Euryarchaeota/classificação
Filogenia
[Mh] Termos MeSH secundário: Composição de Bases
DNA Arqueal/genética
Egito
Euryarchaeota/genética
Euryarchaeota/isolamento & purificação
Hibridização de Ácido Nucleico
Fosfolipídeos/química
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (Phospholipids); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180212
[Lr] Data última revisão:
180212
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180202
[St] Status:MEDLINE
[do] DOI:10.1099/ijsem.0.002524


  2 / 1852 MEDLINE  
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[PMID]:29364600
[Au] Autor:Chernitsyna SM; Khal'zov IA; Khanaeva TA; Morozov IV; Klimenkov IV; Pimenov NV; Zemskayal TI
[Ti] Título:Microbial Community Associated with Thioploca sp. Sheaths in the Area of the Posolski Bank Methane Seep, Southern Baikal.
[So] Source:Mikrobiologiia;85(5):522-530, 2016 Sep.
[Is] ISSN:0026-3656
[Cp] País de publicação:Russia (Federation)
[La] Idioma:eng
[Ab] Resumo:Bacterial mats formed by a colorless sulfur bacterium Thioploca sp. in the area of the Posolski Bank cold methane seep (southern Baikal) were -studied using electron microscopy and phylogenetic analysis. Morphologically the bacteria were identified as Thioploca ingrica.- Confocal microscopy of DAPI-stained samples revealed numerous rod-shaped, filamentous, and spiral microorganisms in the sheaths, as well as in- side and between the trichomes. Transmission electron microscopy revealed nonvacuolated bacteria and small cells-without cell envelopes within the sheath. Bacteria with pronounced intracytoplasmic membranes characteristic; of type I methanotrophs were observed at the outer side of the sheath. Based on analysis of the 16S rRNA gene sequences, the following phyla were idenified in the sheath community: Bacteroidetes, Nitro- spira, Chloroflexi, Planctomycetes, Verrucomicrobia,'y-, and 6-Proteobacteria, Euryarchaeota, Crenarchaeota, and Thaumarchaeota, as well as anammox bacteria. A hypothetical scheme of matter flows in the Lake Baikal bacterial mats was proposed based on the data on metabolism of the cultured homologues.
[Mh] Termos MeSH primário: Água Subterrânea/microbiologia
Lagos/microbiologia
Consórcios Microbianos/fisiologia
RNA Ribossômico 16S/genética
Thiotrichaceae/genética
[Mh] Termos MeSH secundário: Bacteroidetes/classificação
Bacteroidetes/genética
Bacteroidetes/isolamento & purificação
Chloroflexi/classificação
Chloroflexi/genética
Chloroflexi/isolamento & purificação
Crenarchaeota/classificação
Crenarchaeota/genética
Crenarchaeota/isolamento & purificação
Ecossistema
Euryarchaeota/classificação
Euryarchaeota/genética
Euryarchaeota/isolamento & purificação
Metano/química
Metano/metabolismo
Filogenia
Planctomycetales/classificação
Planctomycetales/genética
Planctomycetales/isolamento & purificação
Proteobactérias/classificação
Proteobactérias/genética
Proteobactérias/isolamento & purificação
Sibéria
Thiotrichaceae/classificação
Thiotrichaceae/isolamento & purificação
Thiotrichaceae/ultraestrutura
Verrucomicrobia/classificação
Verrucomicrobia/genética
Verrucomicrobia/isolamento & purificação
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); OP0UW79H66 (Methane)
[Em] Mês de entrada:1802
[Cu] Atualização por classe:180208
[Lr] Data última revisão:
180208
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:180125
[St] Status:MEDLINE


  3 / 1852 MEDLINE  
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[PMID]:28726643
[Au] Autor:Castro-Fernandez V; Herrera-Morande A; Zamora R; Merino F; Gonzalez-Ordenes F; Padilla-Salinas F; Pereira HM; Brandão-Neto J; Garratt RC; Guixe V
[Ad] Endereço:From the Departamento de Biología, Facultad de Ciencias, Universidad de Chile, Santiago 800003, Chile, vcasfe@ug.uchile.cl.
[Ti] Título:Reconstructed ancestral enzymes reveal that negative selection drove the evolution of substrate specificity in ADP-dependent kinases.
[So] Source:J Biol Chem;292(38):15598-15610, 2017 Sep 22.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:One central goal in molecular evolution is to pinpoint the mechanisms and evolutionary forces that cause an enzyme to change its substrate specificity; however, these processes remain largely unexplored. Using the glycolytic ADP-dependent kinases of archaea, including the orders , , and , as a model and employing an approach involving paleoenzymology, evolutionary statistics, and protein structural analysis, we could track changes in substrate specificity during ADP-dependent kinase evolution along with the structural determinants of these changes. To do so, we studied five key resurrected ancestral enzymes as well as their extant counterparts. We found that a major shift in function from a bifunctional ancestor that could phosphorylate either glucose or fructose 6-phosphate (fructose-6-P) as a substrate to a fructose 6-P-specific enzyme was started by a single amino acid substitution resulting in negative selection with a ground-state mode against glucose and a subsequent 1,600-fold change in specificity of the ancestral protein. This change rendered the residual phosphorylation of glucose a promiscuous and physiologically irrelevant activity, highlighting how promiscuity may be an evolutionary vestige of ancestral enzyme activities, which have been eliminated over time. We also could reconstruct the evolutionary history of substrate utilization by using an evolutionary model of discrete binary characters, indicating that substrate uses can be discretely lost or acquired during enzyme evolution. These findings exemplify how negative selection and subtle enzyme changes can lead to major evolutionary shifts in function, which can subsequently generate important adaptive advantages, for example, in improving glycolytic efficiency in .
[Mh] Termos MeSH primário: Complexos de ATP Sintetase/metabolismo
Evolução Molecular
[Mh] Termos MeSH secundário: Complexos de ATP Sintetase/química
Complexos de ATP Sintetase/genética
Sequência de Aminoácidos
Euryarchaeota/enzimologia
Frutosefosfatos/metabolismo
Glucose/metabolismo
Cinética
Modelos Moleculares
Mutação
Filogenia
Conformação Proteica
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fructosephosphates); 6814-87-5 (fructose-6-phosphate); EC 2.7.4.- (ATP Synthetase Complexes); IY9XDZ35W2 (Glucose)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171009
[Lr] Data última revisão:
171009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170721
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M117.790865


  4 / 1852 MEDLINE  
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[PMID]:28709074
[Au] Autor:Yeung T; Kwan M; Adler L; Mills TJ; Neilan BA; Conibeer G; Patterson R
[Ad] Endereço:School of Photovoltaics and Renewable Energy Engineering, University of New South Wales, Sydney, NSW 2052, Australia. Electronic address: tracey.yeung@unsw.edu.au.
[Ti] Título:Increased methane production in cyanobacteria and methanogenic microbe co-cultures.
[So] Source:Bioresour Technol;243:686-692, 2017 Nov.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel light-to-bioenergy system produced 3.5 times the baseline methane output using a co-culture of cyanobacteria (Oscillatoria sp.) and a methanogenic microbial community. Analysis of micronutrients in the system during the growth phase indicated that cobalt, iron, nickel and zinc were not appreciably consumed. The stable consumption and return of macronutrients calcium and magnesium were also observed. Essential macronutrients nitrogen, in the form of nitrate, and phosphorus showed no cycling during the growth phase and were depleted at rates of 0.35mg/L/day and 0.40µg/L/day, respectively. Biofilm formation increased the resilience of biomass to bacterial degradation in an anaerobic digester, as shown by viability assays of cyanobacterial biofilms in the co-culture.
[Mh] Termos MeSH primário: Cianobactérias
Metano
[Mh] Termos MeSH secundário: Biofilmes
Crescimento Quimioautotrófico
Técnicas de Cocultura
Euryarchaeota
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
OP0UW79H66 (Methane)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170715
[St] Status:MEDLINE


  5 / 1852 MEDLINE  
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[PMID]:28672211
[Au] Autor:Li P; Jiang Z; Wang Y; Deng Y; Van Nostrand JD; Yuan T; Liu H; Wei D; Zhou J
[Ad] Endereço:State Key Laboratory of Biogeology and Environmental Geology, China University of Geosciences, Wuhan 430074, PR China. Electronic address: pli@cug.edu.cn.
[Ti] Título:Analysis of the functional gene structure and metabolic potential of microbial community in high arsenic groundwater.
[So] Source:Water Res;123:268-276, 2017 Oct 15.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Microbial functional potential in high arsenic (As) groundwater ecosystems remains largely unknown. In this study, the microbial community functional composition of nineteen groundwater samples was investigated using a functional gene array (GeoChip 5.0). Samples were divided into low and high As groups based on the clustering analysis of geochemical parameters and microbial functional structures. The results showed that As related genes (arsC, arrA), sulfate related genes (dsrA and dsrB), nitrogen cycling related genes (ureC, amoA, and hzo) and methanogen genes (mcrA, hdrB) in groundwater samples were correlated with As, SO , NH or CH concentrations, respectively. Canonical correspondence analysis (CCA) results indicated that some geochemical parameters including As, total organic content, SO , NH , oxidation-reduction potential (ORP) and pH were important factors shaping the functional microbial community structures. Alkaline and reducing conditions with relatively low SO , ORP, and high NH , as well as SO and Fe reduction and ammonification involved in microbially-mediated geochemical processes could be associated with As enrichment in groundwater. This study provides an overall picture of functional microbial communities in high As groundwater aquifers, and also provides insights into the critical role of microorganisms in As biogeochemical cycling.
[Mh] Termos MeSH primário: Arsênico/química
Genes Bacterianos
Poluentes Químicos da Água
[Mh] Termos MeSH secundário: Euryarchaeota
Variação Genética
Água Subterrânea
Microbiologia do Solo
Sulfatos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Sulfates); 0 (Water Pollutants, Chemical); N712M78A8G (Arsenic)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170704
[St] Status:MEDLINE


  6 / 1852 MEDLINE  
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[PMID]:28604769
[Au] Autor:Da Cunha V; Gaia M; Gadelle D; Nasir A; Forterre P
[Ad] Endereço:Institut Pasteur, Unité de Biologie Moléculaire du Gène chez les Extrêmophiles (BMGE), Département de Microbiologie Paris, France.
[Ti] Título:Lokiarchaea are close relatives of Euryarchaeota, not bridging the gap between prokaryotes and eukaryotes.
[So] Source:PLoS Genet;13(6):e1006810, 2017 Jun.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The eocyte hypothesis, in which Eukarya emerged from within Archaea, has been boosted by the description of a new candidate archaeal phylum, "Lokiarchaeota", from metagenomic data. Eukarya branch within Lokiarchaeota in a tree reconstructed from the concatenation of 36 universal proteins. However, individual phylogenies revealed that lokiarchaeal proteins sequences have different evolutionary histories. The individual markers phylogenies revealed at least two subsets of proteins, either supporting the Woese or the Eocyte tree of life. Strikingly, removal of a single protein, the elongation factor EF2, is sufficient to break the Eukaryotes-Lokiarchaea affiliation. Our analysis suggests that the three lokiarchaeal EF2 proteins have a chimeric organization that could be due to contamination and/or homologous recombination with patches of eukaryotic sequences. A robust phylogenetic analysis of RNA polymerases with a new dataset indicates that Lokiarchaeota and related phyla of the Asgard superphylum are sister group to Euryarchaeota, not to Eukarya, and supports the monophyly of Archaea with their rooting in the branch leading to Thaumarchaeota.
[Mh] Termos MeSH primário: Eucariotos/genética
Euryarchaeota/genética
Evolução Molecular
Filogenia
[Mh] Termos MeSH secundário: Proteínas Arqueais/genética
Células Procarióticas
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Archaeal Proteins)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170726
[Lr] Data última revisão:
170726
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170613
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006810


  7 / 1852 MEDLINE  
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[PMID]:28594863
[Au] Autor:Köstner N; Scharnreitner L; Jürgens K; Labrenz M; Herndl GJ; Winter C
[Ad] Endereço:Department of Limnology and Bio-Oceanography, University of Vienna, Vienna, Austria.
[Ti] Título:High viral abundance as a consequence of low viral decay in the Baltic Sea redoxcline.
[So] Source:PLoS One;12(6):e0178467, 2017.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Throughout the Baltic Sea redoxcline, virus production and the frequency of lytically-infected prokaryotic cells were estimated from parallel incubations of undiluted seawater and seawater that contained prokaryotes with substantially reduced numbers of viruses (virus dilution approach), effectively preventing viral reinfection during the incubation period. Undiluted seawater incubations resulted in much higher estimates of virus production (6-35×104 mL-1 h-1) and the frequency of infected cells (5-84%) than the virus dilution approach (virus production: 1-3×104 mL-1 h-1; frequency of infected cells: 1-11%). Viral production and the frequency of infected cells from both approaches, however, cannot be directly compared, as data obtained from undiluted incubations were biased by viral reinfection and other uncontrollable processes during the incubation period. High in situ viral abundance (1-2×107 mL-1) together with low virus production rates based on the virus dilution approach resulted in some of the longest viral turnover times (24-84 d) ever reported for the epipelagial. Throughout a wide range of environmental conditions, viral turnover time and burst size were negatively correlated. Given that viral decay estimated in ultra-filtered water was below the detection limit and the burst size was low (1-17), we conclude that prokaryotic viruses in the Baltic Sea redoxcline are investing most of their resources into stress defense (strong capsids) rather than proliferation (high burst size). In summary, the Baltic Sea redoxcline constitutes an environment where low virus production is found in combination with low viral decay, resulting in high viral abundance.
[Mh] Termos MeSH primário: Água do Mar/virologia
Vírus/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Bactérias/crescimento & desenvolvimento
Biodiversidade
Crenarchaeota/crescimento & desenvolvimento
Euryarchaeota/crescimento & desenvolvimento
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170609
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0178467


  8 / 1852 MEDLINE  
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[PMID]:28477516
[Au] Autor:Zhao Y; Wu J; Yuan X; Zhu W; Wang X; Cheng X; Cui Z
[Ad] Endereço:College of Agronomy and Biotechnology, China Agricultural University, Beijing 100193, China.
[Ti] Título:The effect of mixing intensity on the performance and microbial dynamics of a single vertical reactor integrating acidogenic and methanogenic phases in lignocellulosic biomass digestion.
[So] Source:Bioresour Technol;238:542-551, 2017 Aug.
[Is] ISSN:1873-2976
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The ready formation of scum in vertical reactors has been a bottleneck in the digestion of lignocellulosic materials for biogas production. This study describes a single vertical reactor that integrates the acidogenic and methanogenic phases of this process. The effects of two types of maize stover feedstock (fresh and silage) and two mixing intensities (20 and 70rpm) on methane yield were orthogonally determined. Fresh maize stover yielded approximately 14% more methane than silage maize stover. Mixing at 20rpm contributed to methane yield, while mixing at 70rpm blurred the phase boundary, resulting in accumulation of volatile fatty acids and loss of methanogens. The upper and lower phases clearly constituted a two-phase fermentation system. Clostridiales occupied the acidogenic phase, while the predominant bacteria in the methanogenic phase were Bacteroidetes, Chloroflexi, and Synergistetes. The absolute predominance of Methanosaetaceae clearly demonstrated that aceticlastic methanogenesis was the main route of methane production.
[Mh] Termos MeSH primário: Reatores Biológicos
Metano
[Mh] Termos MeSH secundário: Biomassa
Euryarchaeota
Methanosarcinales
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
OP0UW79H66 (Methane)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171023
[Lr] Data última revisão:
171023
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170507
[St] Status:MEDLINE


  9 / 1852 MEDLINE  
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[PMID]:28402870
[Au] Autor:Ziels RM; Beck DAC; Stensel HD
[Ad] Endereço:Department of Civil and Environmental Engineering, University of Washington, Seattle, WA, USA; Department of Civil Engineering, University of British Columbia, Vancouver, Canada. Electronic address: ziels@mail.ubc.ca.
[Ti] Título:Long-chain fatty acid feeding frequency in anaerobic codigestion impacts syntrophic community structure and biokinetics.
[So] Source:Water Res;117:218-229, 2017 Jun 15.
[Is] ISSN:1879-2448
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:This study investigated the impacts of long-chain fatty acid (LCFA) feeding frequencies on microbial community structure, bioconversion kinetics, and process stability during anaerobic codigestion. Parallel laboratory-scale anaerobic codigesters fed with dairy cattle manure were either pulse-fed every two days or continuously-fed daily, respectively, with oleate (C ) in incremental step increases over 200 days up to 64% of the influent chemical oxygen demand (COD). The effluent acetate concentration exceeded 3000 mg/L in the continuous-fed codigester at the highest oleate loading rate, but remained below 100 mg/L in the pulse-fed codigester at the end of its 48-hr oleate feed cycle. Maximum substrate conversion rates of oleate (q ) and acetate (q ) were significantly higher in the pulse-fed codigester compared to the continuous-fed codigester. 16S rRNA gene amplicon sequencing showed that Bacteria and Archaea community profiles diverged based on the codigester LCFA feeding pattern and loading rate. LCFA-degrading Syntrophomonas bacteria were significantly enriched in both LCFA codigesters relative to the control digester. The pulse-fed codigester had the highest community fraction of Syntrophomonas 16S rRNA genes by the end of the experiment with 43% of Bacteria amplicon sequences. q and q values were both significantly correlated to absolute concentrations of Syntrophomonas and Methanosaeta 16S rRNA genes, respectively. Multiple-linear regression models based on the absolute abundance of Syntrophomonas and Methanosaeta taxa provided improved predictions of oleate and acetate bioconversion kinetics, respectively. These results collectively suggest that pulse feeding rather than continuous feeding LCFA during anaerobic codigestion selected for higher microbial bioconversion kinetics and functional stability, which were related to changes in the physiological diversity and adaptive capacity of syntrophic and methanogenic communities.
[Mh] Termos MeSH primário: Bactérias Anaeróbias/genética
RNA Ribossômico 16S/genética
[Mh] Termos MeSH secundário: Anaerobiose
Animais
Archaea/genética
Reatores Biológicos/microbiologia
Euryarchaeota/genética
Ácidos Graxos
Metano
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Fatty Acids); 0 (RNA, Ribosomal, 16S); OP0UW79H66 (Methane)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170505
[Lr] Data última revisão:
170505
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170414
[St] Status:MEDLINE


  10 / 1852 MEDLINE  
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[PMID]:28370935
[Au] Autor:Wang Q; Dore JE; McDermott TR
[Ad] Endereço:Department of Land Resources and Environmental Sciences, Montana State University, Bozeman, MT, 59717, USA.
[Ti] Título:Methylphosphonate metabolism by Pseudomonas sp. populations contributes to the methane oversaturation paradox in an oxic freshwater lake.
[So] Source:Environ Microbiol;19(6):2366-2378, 2017 Jun.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The 'CH oversaturation paradox' has been observed in oxygen-rich marine and lake waters, and viewed to significantly contribute to biosphere cycling of methane, a potent greenhouse gas. Our study focused on the intriguing well-defined pelagic methane enriched zone (PMEZ) in freshwater lakes. Spiking Yellowstone Lake PMEZ samples with C-labeled potential methanogenesis substrates found only C-methylphosphonate (MPn) resulted in CH generation. In 16S rRNA gene Illumina libraries, four Pseudomonas sp. operational taxonomic units surprisingly accounted for ∼11% abundance in the PMEZ community. Pseudomonas sp. isolates were also obtained from MPn enrichments with PMEZ water; they were most aggressive in MPn metabolism and their 16S rRNA gene sequences matched 35% of the Illumina PMEZ Pseudomonas reads. Further, two key genes encoding C-P lyase (phnJL, an important enzyme for dealkylation of MPn), were only amplifiable from PMEZ DNA and all PCR generated phnJL clones matched those of the Pseudomonas sp. isolates. Notably, methanogen 16S rRNA signatures were absent in all Illumina libraries and mcrA was not detected via PCR. Collectively, these observations are consistent with the conclusion that MPn metabolism contributes significantly to CH oversaturation in Yellowstone Lake and likely other oxic freshwater lake environments, and that Pseudomonas sp. populations are critical participants.
[Mh] Termos MeSH primário: Lagos/química
Metano/metabolismo
Compostos Organofosforados/metabolismo
Pseudomonas/metabolismo
[Mh] Termos MeSH secundário: Enzimas de Restrição do DNA/genética
Euryarchaeota/genética
Lagos/microbiologia
Liases/genética
Filogenia
RNA Ribossômico 16S/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Organophosphorus Compounds); 0 (RNA, Ribosomal, 16S); 329W4YM10Z (methylphosphonic acid); EC 3.1.21.- (DNA Restriction Enzymes); EC 3.1.21.- (endodeoxyribonuclease McrA); EC 4.- (Lyases); EC 4.99.- (carbon-phosphorus lyase); OP0UW79H66 (Methane)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170404
[St] Status:MEDLINE
[do] DOI:10.1111/1462-2920.13747



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