Base de dados : MEDLINE
Pesquisa : B02.200.080.080 [Categoria DeCS]
Referências encontradas : 26 [refinar]
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  1 / 26 MEDLINE  
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[PMID]:27795311
[Au] Autor:Rodrigues MV; Borges N; Santos H
[Ad] Endereço:Instituto de Tecnologia Química e Biológica António Xavier, Universidade Nova de Lisboa, Oeiras, Portugal.
[Ti] Título:Glycerol Phosphate Cytidylyltransferase Stereospecificity Is Key to Understanding the Distinct Stereochemical Compositions of Glycerophosphoinositol in Bacteria and Archaea.
[So] Source:Appl Environ Microbiol;83(1), 2017 Jan 01.
[Is] ISSN:1098-5336
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Glycerophosphoinositol (GPI) is a compatible solute present in a few hyperthermophiles. Interestingly, different GPI stereoisomers accumulate in Bacteria and Archaea, and the basis for this domain-dependent specificity was investigated herein. The archaeon Archaeoglobus fulgidus and the bacterium Aquifex aeolicus were used as model organisms. The synthesis of GPI involves glycerol phosphate cytidylyltransferase (GCT), which catalyzes the production of CDP-glycerol from CTP and glycerol phosphate, and di-myo-inositol phosphate-phosphate synthase (DIPPS), catalyzing the formation of phosphorylated GPI from CDP-glycerol and l-myo-inositol 1-phosphate. DIPPS of A. fulgidus recognized the two CDP-glycerol stereoisomers similarly. This feature and the ability of P nuclear magnetic resonance (NMR) to distinguish the GPI diastereomers provided a means to study the stereospecificity of GCTs. The AF1418 gene and genes aq_185 and aq_1368 are annotated as putative GCT genes in the genomes of A. fulgidus and Aq. aeolicus, respectively. The functions of these genes were determined by assaying the activity of the respective recombinant proteins: AQ1368 and AQ185 are GCTs, while AF1418 has flavin adenine dinucleotide (FAD) synthetase activity. AQ185 is absolutely specific for sn-glycerol 3-phosphate, while AQ1368 recognizes the two enantiomers but has a 2:1 preference for sn-glycerol 3-phosphate. In contrast, the partially purified A. fulgidus GCT uses sn-glycerol 1-phosphate preferentially (4:1). Significantly, the predominant GPI stereoforms found in the bacterium and the archaeon reflect the distinct stereospecificities of the respective GCTs: i.e., A. fulgidus accumulates predominantly sn-glycero-1-phospho-3-l-myo-inositol, while Aq. aeolicus accumulates sn-glycero-3-phospho-3-l-myo-inositol. IMPORTANCE: Compatible solutes of hyperthermophiles show high efficacy in thermal protection of proteins in comparison with solutes typical of mesophiles; therefore, they are potentially useful in several biotechnological applications. Glycerophosphoinositol (GPI) is synthesized from CDP-glycerol and l-myo-inositol 1-phosphate in a few hyperthermophiles. In this study, the molecular configuration of the GPI stereoisomers accumulated by members of the Bacteria and Archaea was established. The stereospecificity of glycerol phosphate cytidylyltransferase (GCT), the enzyme catalyzing the synthesis of CDP-glycerol, is crucial to the stereochemistry of GPI. However, the stereospecific properties of GCTs have not been investigated thus far. We devised a method to characterize GCT stereospecificity which does not require sn-glycerol 1-phosphate, a commercially unavailable substrate. This led us to understand the biochemical basis for the distinct GPI stereoisomer composition observed in archaea and bacteria.
[Mh] Termos MeSH primário: Archaeoglobus/enzimologia
Bactérias/enzimologia
Citidina Trifosfato/metabolismo
Fosfatos de Inositol/química
Nucleotidiltransferases/metabolismo
[Mh] Termos MeSH secundário: Archaeoglobus/genética
Archaeoglobus/metabolismo
Bactérias/genética
Bactérias/metabolismo
Citidina Trifosfato/química
Glicerol/metabolismo
Fosfatos de Inositol/metabolismo
Espectroscopia de Ressonância Magnética
Conformação Molecular
Nucleotidiltransferases/química
Proteínas Recombinantes/metabolismo
Estereoisomerismo
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Inositol Phosphates); 0 (Recombinant Proteins); 15421-51-9 (inositol 1-phosphate); 16824-65-0 (glycerylphosphoinositol); 65-47-4 (Cytidine Triphosphate); EC 2.7.7.- (Nucleotidyltransferases); EC 2.7.7.39 (glycerol-3-phosphate cytidylyltransferase); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1711
[Cu] Atualização por classe:171102
[Lr] Data última revisão:
171102
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161101
[St] Status:MEDLINE


  2 / 26 MEDLINE  
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[PMID]:26493287
[Au] Autor:Le CQ; Joseph E; Nguyen T; Johnson-Winters K
[Ti] Título:Optimization of Expression and Purification of Recombinant Archeoglobus fulgidus F420H2:NADP+ Oxidoreductase, an F420 Cofactor Dependent Enzyme.
[So] Source:Protein J;34(6):391-7, 2015 Dec.
[Is] ISSN:1875-8355
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:Methanogens play a critical role in carbon cycling and contain a number of intriguing biosynthetic pathways. One unusual cofactor found in methanogenic and sulfate reducing archaea is Factor 420 (F420), which can be interconverted between its reduced and oxidized forms by the F420H2:NADP(+) oxidoreductase (Fno) through hydride transfer mechanisms. Here, we report an optimized expression and purification method for recombinant Fno derived from the extreme thermophile Archeoglobus fulgidus. An expression vector that is codon-optimized for heterologous expression in Escherichia coli, modified growth conditions, and a modified purification protocol involving a key polyethyleneimine precipitation step results in a highly purified, homogeneous preparation of Fno that displays high catalytic activity with a truncated F420 analog. This method should accelerate studies on how Fno uses the unusual F420 cofactor during catalysis.
[Mh] Termos MeSH primário: Proteínas Arqueais/isolamento & purificação
Proteínas Arqueais/metabolismo
NADH NADPH Oxirredutases/isolamento & purificação
NADH NADPH Oxirredutases/metabolismo
Proteínas Recombinantes/isolamento & purificação
Proteínas Recombinantes/metabolismo
[Mh] Termos MeSH secundário: Proteínas Arqueais/química
Proteínas Arqueais/genética
Archaeoglobus/enzimologia
Archaeoglobus/genética
Escherichia coli/genética
NADH NADPH Oxirredutases/química
NADH NADPH Oxirredutases/genética
NADP/metabolismo
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Archaeal Proteins); 0 (Recombinant Proteins); 53-59-8 (NADP); EC 1.6.- (NADH, NADPH Oxidoreductases); EC 1.6.8.- (F420-dependent NADP reductase)
[Em] Mês de entrada:1609
[Cu] Atualização por classe:171027
[Lr] Data última revisão:
171027
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:151024
[St] Status:MEDLINE


  3 / 26 MEDLINE  
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[PMID]:25152044
[Au] Autor:Shida F; Mizuta S
[Ad] Endereço:Graduate School of Science and Technology, Hirosaki University, 3 Bunkyo-cho, Hirosaki, Aomori 036-8561, Japan.
[Ti] Título:Measurement of word frequencies in genomic DNA sequences based on partial alignment and fuzzy set.
[So] Source:J Bioinform Comput Biol;12(4):1450019, 2014 Aug.
[Is] ISSN:1757-6334
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Accompanied with the rapid increase of the amount of data registered in the databases of biological sequences, the need for a fast method of sequence comparison applicable to sequences of large size is also increasing. In general, alignment is used for sequence comparison. However, the alignment may not be appropriate for comparison of sequences of large size such as whole genome sequences due to its large time complexity. In this article, we propose a semi alignment-free method of sequence comparison based on word frequency distributions, in which we partially use the alignment to measure word frequencies along with the idea of fuzzy set theory. Experiments with ten bacterial genome sequences demonstrated that the fuzzy measurements has the effect that facilitates discrimination between close relatives and distant relatives.
[Mh] Termos MeSH primário: Alinhamento de Sequência/métodos
Análise de Sequência de DNA/métodos
[Mh] Termos MeSH secundário: Archaeoglobus/genética
Bacillus/genética
Escherichia coli K12/genética
Escherichia coli O157/genética
Lógica Fuzzy
Genoma
Genoma Bacteriano
Genômica/métodos
Filogenia
Pyrococcus horikoshii/genética
Vibrio cholerae/genética
Yersinia pestis/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Em] Mês de entrada:1506
[Cu] Atualização por classe:140825
[Lr] Data última revisão:
140825
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140826
[St] Status:MEDLINE
[do] DOI:10.1142/S021972001450019X


  4 / 26 MEDLINE  
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[PMID]:21410513
[Au] Autor:Estelmann S; Ramos-Vera WH; Gad'on N; Huber H; Berg IA; Fuchs G
[Ad] Endereço:Mikrobiologie, Fakultät Biologie, Universität Freiburg, Freiburg, Germany.
[Ti] Título:Carbon dioxide fixation in 'Archaeoglobus lithotrophicus': are there multiple autotrophic pathways?
[So] Source:FEMS Microbiol Lett;319(1):65-72, 2011 Jun.
[Is] ISSN:1574-6968
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Several representatives of the euryarchaeal class Archaeoglobi are able to grow facultative autotrophically using the reductive acetyl-CoA pathway, with 'Archaeoglobus lithotrophicus' being an obligate autotroph. However, genome sequencing revealed that some species harbor genes for key enzymes of other autotrophic pathways, i.e. 4-hydroxybutyryl-CoA dehydratase of the dicarboxylate/hydroxybutyrate cycle and the hydroxypropionate/hydroxybutyrate cycle and ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) of the Calvin-Benson cycle. This raised the question of whether only one or multiple autotrophic pathways are operating in these species. We searched for the presence of enzyme activities specific for the dicarboxylate/hydroxybutyrate or the hydroxypropionate/hydroxybutyrate cycles in 'A. lithotrophicus', but such enzymes could not be detected. Low Rubisco activity was detected that could not account for the carbon dioxide (CO(2)) fixation rate; in addition, phosphoribulokinase activity was not found. The generation of ribulose 1,5-bisphosphate from 5-phospho-D-ribose 1-pyrophosphate was observed, but not from AMP; these sources for ribulose 1,5-bisphosphate have been proposed before. Our data indicate that the reductive acetyl-CoA pathway is the only functioning CO(2) fixation pathway in 'A. lithotrophicus'.
[Mh] Termos MeSH primário: Archaeoglobus/metabolismo
Dióxido de Carbono/metabolismo
[Mh] Termos MeSH secundário: Proteínas Arqueais/genética
Proteínas Arqueais/metabolismo
Archaeoglobus/enzimologia
Archaeoglobus/genética
Processos Autotróficos
Expressão Gênica
Hidroliases/genética
Hidroliases/metabolismo
Hidroxibutiratos/metabolismo
Ribulose-Bifosfato Carboxilase/genética
Ribulose-Bifosfato Carboxilase/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Archaeal Proteins); 0 (Hydroxybutyrates); 142M471B3J (Carbon Dioxide); EC 4.1.1.39 (Ribulose-Bisphosphate Carboxylase); EC 4.2.1.- (4-hydroxybutyryl-CoA dehydratase); EC 4.2.1.- (Hydro-Lyases)
[Em] Mês de entrada:1108
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110318
[St] Status:MEDLINE
[do] DOI:10.1111/j.1574-6968.2011.02268.x


  5 / 26 MEDLINE  
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[PMID]:20061497
[Au] Autor:Steinsbu BO; Thorseth IH; Nakagawa S; Inagaki F; Lever MA; Engelen B; Øvreås L; Pedersen RB
[Ad] Endereço:Centre for Geobiology, University of Bergen, Allégaten 41, N-5007 Bergen, Norway. bjorn.steinsbu@geo.uib.no
[Ti] Título:Archaeoglobus sulfaticallidus sp. nov., a thermophilic and facultatively lithoautotrophic sulfate-reducer isolated from black rust exposed to hot ridge flank crustal fluids.
[So] Source:Int J Syst Evol Microbiol;60(Pt 12):2745-52, 2010 Dec.
[Is] ISSN:1466-5034
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel thermophilic and lithoautotrophic sulfate-reducing archaeon was isolated from black rust formed on the steel surface of a borehole observatory (CORK 1026B) retrieved during IODP Expedition 301 on the eastern flank of Juan de Fuca Ridge, eastern Pacific Ocean. Cells of the strain were lobe-shaped or triangular. The optimum temperature, pH and NaCl concentration for growth were 75°C, pH 7 and 2 % (w/v), respectively. The isolate was strictly anaerobic, growing lithoautotrophically on H(2) and CO(2) using sulfate, sulfite or thiosulfate as electron acceptors. Lactate and pyruvate could serve as alternative energy and carbon sources. The G+C content of the genomic DNA was 42 mol%. Phylogenetic analyses of the 16S rRNA gene indicated that the isolate was closely related to members of the family Archaeoglobaceae, with sequence similarities of 90.3-94.4 %. Physiological and molecular properties showed that the isolate represents a novel species of the genus Archaeoglobus. The name Archaeoglobus sulfaticallidus sp. nov. is proposed; the type strain is PM70-1(T) (=DSM 19444(T)=JCM 14716(T)).
[Mh] Termos MeSH primário: Archaeoglobus/classificação
Filogenia
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: Archaeoglobus/genética
Archaeoglobus/isolamento & purificação
Técnicas de Tipagem Bacteriana
Composição de Bases
DNA Arqueal/genética
Temperatura Alta
Dados de Sequência Molecular
Oxirredução
Oceano Pacífico
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Sulfatos/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (RNA, Ribosomal, 16S); 0 (Sulfates)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:101214
[Lr] Data última revisão:
101214
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:100112
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.016105-0


  6 / 26 MEDLINE  
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[PMID]:18398174
[Au] Autor:Mori K; Maruyama A; Urabe T; Suzuki K; Hanada S
[Ad] Endereço:NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
[Ti] Título:Archaeoglobus infectus sp. nov., a novel thermophilic, chemolithoheterotrophic archaeon isolated from a deep-sea rock collected at Suiyo Seamount, Izu-Bonin Arc, western Pacific Ocean.
[So] Source:Int J Syst Evol Microbiol;58(Pt 4):810-6, 2008 Apr.
[Is] ISSN:1466-5026
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:A novel thermophilic, strictly anaerobic archaeon, designated strain Arc51T, was isolated from a rock sample collected from a deep-sea hydrothermal field in Suiyo Seamount, Izu-Bonin Arc, western Pacific Ocean. Cells of the isolate were irregular cocci with single flagella and exhibited blue-green fluorescence at 436 nm. The optimum temperature, pH and NaCl concentration for growth were 70 degrees C, pH 6.5 and 3 % (w/v), respectively. Strain Arc51T could grow on thiosulfate or sulfite as an electron acceptor in the presence of hydrogen. This strain required acetate as a carbon source for its growth, suggesting that the reductive acetyl CoA pathway for CO2 fixation was incomplete. In addition, coenzyme M (2-mercaptoethanesulfonic acid), which is a known methyl carrier in methanogenesis, was also a requirement for growth of the strain. Analysis of the 16S rRNA gene sequence revealed that the isolate was similar to members of the genus Archaeoglobus, with sequence similarities of 93.6-97.2 %; the closest relative was Archaeoglobus veneficus. Phylogenetic analyses of the dsrAB and apsA genes, encoding the alpha and beta subunits of dissimilatory sulfite reductase and the alpha subunit of adenosine-5'-phosphosulfate reductase, respectively, produced results similar to those inferred from comparisons based on the 16S rRNA gene sequence. On the basis of phenotypic and phylogenetic data, strain Arc51T represents a novel species of the genus Archaeoglobus, for which the name Archaeoglobus infectus sp. nov. is proposed. The type strain is Arc51T (=NBRC 100649T=DSM 18877T).
[Mh] Termos MeSH primário: Archaeoglobus/classificação
Archaeoglobus/isolamento & purificação
[Mh] Termos MeSH secundário: Archaeoglobus/genética
Archaeoglobus/metabolismo
Composição de Bases
Sequência de Bases
Primers do DNA/genética
DNA Bacteriano/química
DNA Bacteriano/genética
Genes Bacterianos
Sedimentos Geológicos/microbiologia
Temperatura Alta
Mesna/metabolismo
Microscopia Eletrônica de Transmissão
Dados de Sequência Molecular
Oceano Pacífico
Fenótipo
Filogenia
RNA Bacteriano/genética
RNA Ribossômico 16S/genética
Água do Mar/microbiologia
Especificidade da Espécie
Terminologia como Assunto
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA Primers); 0 (DNA, Bacterial); 0 (RNA, Bacterial); 0 (RNA, Ribosomal, 16S); NR7O1405Q9 (Mesna)
[Em] Mês de entrada:0807
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080410
[St] Status:MEDLINE
[do] DOI:10.1099/ijs.0.65422-0


  7 / 26 MEDLINE  
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[PMID]:17253090
[Au] Autor:Payandeh J; Pai EF
[Ad] Endereço:Department of Medical Biophysics, University of Toronto, Toronto, Ontario, Canada. pai@hera.med.utoronto.ca
[Ti] Título:Enzyme-driven speciation: crystallizing Archaea via lipid capture.
[So] Source:J Mol Evol;64(3):364-74, 2007 Mar.
[Is] ISSN:0022-2844
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:As the origin(s) of life on Earth remains an open question, detailed characteristics about the "last universal ancestor" (LUA) continue to be obscured. Here we provide arguments that strengthen the bacterial-like nature of the LUA. Our view attempts to recreate the evolution of archaeal lipids, the major components of the distinctive membrane that encapsulates these ancient prokaryotes. We show that (S)- 3-O-geranylgeranylglyceryl phosphate synthase (GGGPS), a TIM-barrel protein that performs the committed step in archaeal lipid synthesis, likely evolved from the duplication and fusion of a (betaalpha)4 half-barrel ancestor. By comparison to the well-characterized HisA and HisF TIM-barrel proteins, we propose a time line for the invention of this diagnostic archaeal biosynthetic pathway. After excluding the possibility of horizontal gene transfer, we conclude that the evolutionary history of GGGPS mirrors the emergence of Archaea from the LUA. We illustrate aspects of this "lipid capture" model that support its likelihood in recreating key evolutionary events and, as our hypothesis is built on a single initiating event, we suggest that the appearance of GGGPS represents an example of enzyme-driven speciation.
[Mh] Termos MeSH primário: Alquil e Aril Transferases/genética
Archaea/genética
Proteínas Arqueais/genética
Evolução Molecular
Lipídeos de Membrana/biossíntese
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Archaea/enzimologia
Archaeoglobus/enzimologia
Archaeoglobus/genética
Genoma Arqueal
Lipídeos de Membrana/química
Modelos Moleculares
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Archaeal Proteins); 0 (Membrane Lipids); EC 2.5.- (Alkyl and Aryl Transferases); EC 2.5.1.41 (phosphoglycerol geranylgeranyltransferase)
[Em] Mês de entrada:0709
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070127
[St] Status:MEDLINE


  8 / 26 MEDLINE  
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[PMID]:17245576
[Au] Autor:Kaster KM; Grigoriyan A; Jenneman G; Jennneman G; Voordouw G
[Ad] Endereço:International Research Institute of Stavanger (IRIS), Akvamiljø, Mekjarvik 12, Randaberg, Norway.
[Ti] Título:Effect of nitrate and nitrite on sulfide production by two thermophilic, sulfate-reducing enrichments from an oil field in the North Sea.
[So] Source:Appl Microbiol Biotechnol;75(1):195-203, 2007 May.
[Is] ISSN:0175-7598
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Thermophilic sulfate-reducing bacteria (tSRB) can be major contributors to the production of H(2)S (souring) in oil reservoirs. Two tSRB enrichments from a North Sea oil field, NS-tSRB1 and NS-tSRB2, were obtained at 58 degrees C with acetate-propionate-butyrate and with lactate as the electron donor, respectively. Analysis by rDNA sequencing indicated the presence of Thermodesulforhabdus norvegicus in NS-tSRB1 and of Archaeoglobus fulgidus in NS-tSRB2. Nitrate (10 mM) had no effect on H(2)S production by mid-log phase cultures of NS-tSRB1 and NS-tSRB2, whereas nitrite (0.25 mM or higher) inhibited sulfate reduction. NS-tSRB1 did not recover from inhibition, whereas sulfate reduction activity of NS-tSRB2 recovered after 500 h. Nitrite was also effective in souring inhibition and H(2)S removal in upflow bioreactors, whereas nitrate was similarly ineffective. Hence, nitrite may be preferable for souring prevention in some high-temperature oil fields because it reacts directly with sulfide and provides long-lasting inhibition of sulfate reduction.
[Mh] Termos MeSH primário: Archaeoglobus
Deltaproteobacteria
Óleos Combustíveis
Nitratos/farmacologia
Nitritos/farmacologia
Sulfetos/metabolismo
[Mh] Termos MeSH secundário: Archaeoglobus/classificação
Archaeoglobus/genética
Archaeoglobus/isolamento & purificação
Archaeoglobus/metabolismo
Deltaproteobacteria/classificação
Deltaproteobacteria/genética
Deltaproteobacteria/isolamento & purificação
Deltaproteobacteria/metabolismo
Temperatura Alta
Dados de Sequência Molecular
Mar do Norte
Água do Mar/microbiologia
Análise de Sequência de DNA
Sulfatos/metabolismo
Bactérias Redutoras de Enxofre/classificação
Bactérias Redutoras de Enxofre/genética
Bactérias Redutoras de Enxofre/isolamento & purificação
Bactérias Redutoras de Enxofre/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fuel Oils); 0 (Nitrates); 0 (Nitrites); 0 (Sulfates); 0 (Sulfides)
[Em] Mês de entrada:0707
[Cu] Atualização por classe:081121
[Lr] Data última revisão:
081121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:070125
[St] Status:MEDLINE


  9 / 26 MEDLINE  
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[PMID]:16730457
[Au] Autor:Djuranovic S; Rockel B; Lupas AN; Martin J
[Ad] Endereço:Department of Protein Evolution, Max-Planck-Institute for Developmental Biology, Spemannstrasse 35, D-72076 Tübingen, Germany.
[Ti] Título:Characterization of AMA, a new AAA protein from Archaeoglobus and methanogenic archaea.
[So] Source:J Struct Biol;156(1):130-8, 2006 Oct.
[Is] ISSN:1047-8477
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:We have previously reported a new group of AAA proteins, which is only found in Archaeoglobus and methanogenic archaea (AMA). The proteins are phylogenetically basal to the metalloprotease clade and their N-terminal domain is homologous to the beta-clam part of the N-domain of CDC48-like proteins. Here we report the biochemical and biophysical characterization of Archaeoglobus fulgidus AMA, and of its isolated N-terminal (AMA-N) and ATPase (AMA-DeltaN) domains. AfAMA forms hexameric complexes, as does AMA-N, while AMA-DeltaN only forms dimers. The ability to hexamerize is dependent on the integrity of a GYPL motif in AMA-N, which resembles the pore motif of FtsH and HslU. While the physiological function of AMA is unknown, we show that it has ATP-dependent chaperone activity and can prevent the thermal aggregation of proteins in vitro. The ability to interact with non-native proteins resides in the N-domain and is energy-independent.
[Mh] Termos MeSH primário: Adenosina Trifosfatases/química
Adenosina Trifosfatases/metabolismo
Archaea/enzimologia
Proteínas Arqueais/química
Proteínas Arqueais/metabolismo
Archaeoglobus/enzimologia
[Mh] Termos MeSH secundário: Adenosina Trifosfatases/genética
Adenosina Trifosfatases/isolamento & purificação
Adenosina Trifosfatases/ultraestrutura
Motivos de Aminoácidos
Sequência de Aminoácidos
Proteínas Arqueais/genética
Proteínas Arqueais/isolamento & purificação
Proteínas Arqueais/ultraestrutura
Dicroísmo Circular
Clonagem Molecular
Estabilidade Enzimática
Dados de Sequência Molecular
Peso Molecular
Filogenia
Ligação Proteica
Estrutura Secundária de Proteína
Estrutura Terciária de Proteína
Proteínas Recombinantes/metabolismo
Homologia de Sequência de Aminoácidos
Temperatura Ambiente
[Pt] Tipo de publicação:COMPARATIVE STUDY; JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Archaeal Proteins); 0 (Recombinant Proteins); EC 3.6.1.- (Adenosine Triphosphatases)
[Em] Mês de entrada:0612
[Cu] Atualização por classe:061009
[Lr] Data última revisão:
061009
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:060530
[St] Status:MEDLINE


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[PMID]:16204562
[Au] Autor:Lloyd KG; Edgcomb VP; Molyneaux SJ; Böer S; Wirsen CO; Atkins MS; Teske A
[Ad] Endereço:CB# 3300, Department of Marine Sciences, University of North Carolina-Chapel Hill, Chapel Hill, NC 27599, USA. klloyd@email.unc.edu
[Ti] Título:Effects of dissolved sulfide, pH, and temperature on growth and survival of marine hyperthermophilic Archaea.
[So] Source:Appl Environ Microbiol;71(10):6383-7, 2005 Oct.
[Is] ISSN:0099-2240
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The ability of metabolically diverse hyperthermophilic archaea to withstand high temperatures, low pHs, high sulfide concentrations, and the absence of carbon and energy sources was investigated. Close relatives of our study organisms, Methanocaldococcus jannaschii, Archaeoglobus profundus, Thermococcus fumicolans, and Pyrococcus sp. strain GB-D, are commonly found in hydrothermal vent chimney walls and hot sediments and possibly deeper in the subsurface, where highly dynamic hydrothermal flow patterns and steep chemical and temperature gradients provide an ever-changing mosaic of microhabitats. These organisms (with the possible exception of Pyrococcus strain GB-D) tolerated greater extremes of low pH, high sulfide concentration, and high temperature when actively growing and metabolizing than when starved of carbon sources and electron donors/acceptors. Therefore these organisms must be actively metabolizing in the hydrothermal vent chimneys, sediments, and subsurface in order to withstand at least 24 h of exposure to extremes of pH, sulfide, and temperature that occur in these environments.
[Mh] Termos MeSH primário: Euryarchaeota/crescimento & desenvolvimento
Temperatura Alta
Água do Mar/microbiologia
Sulfetos/farmacologia
[Mh] Termos MeSH secundário: Archaeoglobus/efeitos dos fármacos
Archaeoglobus/crescimento & desenvolvimento
Archaeoglobus/fisiologia
Euryarchaeota/efeitos dos fármacos
Euryarchaeota/fisiologia
Resposta ao Choque Térmico
Concentração de Íons de Hidrogênio
Thermococcus/efeitos dos fármacos
Thermococcus/crescimento & desenvolvimento
Thermococcus/fisiologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Sulfides)
[Em] Mês de entrada:0512
[Cu] Atualização por classe:170219
[Lr] Data última revisão:
170219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:051006
[St] Status:MEDLINE



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