Base de dados : MEDLINE
Pesquisa : B02.200.825 [Categoria DeCS]
Referências encontradas : 29 [refinar]
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  1 / 29 MEDLINE  
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[PMID]:28628615
[Au] Autor:Cossu M; Badel C; Catchpole R; Gadelle D; Marguet E; Barbe V; Forterre P; Oberto J
[Ad] Endereço:Institute for Integrative Biology of the Cell (I2BC), Microbiology Department, CEA, CNRS, Univ. Paris-Sud, Université Paris-Saclay, Gif-sur-Yvette, France.
[Ti] Título:Flipping chromosomes in deep-sea archaea.
[So] Source:PLoS Genet;13(6):e1006847, 2017 Jun.
[Is] ISSN:1553-7404
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:One of the major mechanisms driving the evolution of all organisms is genomic rearrangement. In hyperthermophilic Archaea of the order Thermococcales, large chromosomal inversions occur so frequently that even closely related genomes are difficult to align. Clearly not resulting from the native homologous recombination machinery, the causative agent of these inversions has remained elusive. We present a model in which genomic inversions are catalyzed by the integrase enzyme encoded by a family of mobile genetic elements. We characterized the integrase from Thermococcus nautili plasmid pTN3 and showed that besides canonical site-specific reactions, it catalyzes low sequence specificity recombination reactions with the same outcome as homologous recombination events on DNA segments as short as 104bp both in vitro and in vivo, in contrast to other known tyrosine recombinases. Through serial culturing, we showed that the integrase-mediated divergence of T. nautili strains occurs at an astonishing rate, with at least four large-scale genomic inversions appearing within 60 generations. Our results and the ubiquitous distribution of pTN3-like integrated elements suggest that a major mechanism of evolution of an entire order of Archaea results from the activity of a selfish mobile genetic element.
[Mh] Termos MeSH primário: Inversão Cromossômica/genética
Evolução Molecular
Integrases/genética
Thermococcales/genética
[Mh] Termos MeSH secundário: Genoma Arqueal
Sequências Repetitivas Dispersas/genética
Plasmídeos/genética
Recombinação Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 2.7.7.- (Integrases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170719
[Lr] Data última revisão:
170719
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170620
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pgen.1006847


  2 / 29 MEDLINE  
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[PMID]:27687225
[Au] Autor:Kim M; Park S; Lee SJ
[Ad] Endereço:Department of Biology, Kyung Hee University, Seoul, 02447, Republic of Korea.
[Ti] Título:Global transcriptional regulator TrmB family members in prokaryotes.
[So] Source:J Microbiol;54(10):639-45, 2016 Oct.
[Is] ISSN:1976-3794
[Cp] País de publicação:Korea (South)
[La] Idioma:eng
[Ab] Resumo:Members of the TrmB family act as global transcriptional regulators for the activation or repression of sugar ABC transporters and central sugar metabolic pathways, including glycolytic, gluconeogenic, and other metabolic pathways, and also as chromosomal stabilizers in archaea. As a relatively newly classified transcriptional regulator family, there is limited experimental evidence for their role in Thermococcales, halophilic archaeon Halobacterium salinarum NRC1, and crenarchaea Sulfolobus strains, despite being one of the extending protein families in archaea. Recently, the protein structures of Pyrococcus furiosus TrmB and TrmBL2 were solved, and the transcriptomic data uncovered by microarray and ChIP-Seq were published. In the present review, recent evidence of the functional roles of TrmB family members in archaea is explained and extended to bacteria.
[Mh] Termos MeSH primário: Archaea/genética
Proteínas Arqueais/genética
Bactérias/genética
Elementos Reguladores de Transcrição
Proteínas Repressoras/genética
Fatores de Transcrição/genética
[Mh] Termos MeSH secundário: Transportadores de Cassetes de Ligação de ATP/genética
Archaea/classificação
Bactérias/classificação
Metabolismo dos Carboidratos/genética
Perfilação da Expressão Gênica
Halobacterium salinarum/genética
Pyrococcus furiosus/genética
Proteínas Repressoras/química
Proteínas Repressoras/metabolismo
Thermococcales/classificação
Thermococcales/genética
Fatores de Transcrição/classificação
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Archaeal Proteins); 0 (Repressor Proteins); 0 (Transcription Factors)
[Em] Mês de entrada:1703
[Cu] Atualização por classe:171109
[Lr] Data última revisão:
171109
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161001
[St] Status:MEDLINE
[do] DOI:10.1007/s12275-016-6362-7


  3 / 29 MEDLINE  
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[PMID]:26166067
[Au] Autor:Cossu M; Da Cunha V; Toffano-Nioche C; Forterre P; Oberto J
[Ad] Endereço:Institute of Integrative Cellular Biology, CEA, CNRS, Université Paris Sud, 91405 Orsay, France.
[Ti] Título:Comparative genomics reveals conserved positioning of essential genomic clusters in highly rearranged Thermococcales chromosomes.
[So] Source:Biochimie;118:313-21, 2015 Nov.
[Is] ISSN:1638-6183
[Cp] País de publicação:France
[La] Idioma:eng
[Ab] Resumo:The genomes of the 21 completely sequenced Thermococcales display a characteristic high level of rearrangements. As a result, the prediction of their origin and termination of replication on the sole basis of chromosomal DNA composition or skew is inoperative. Using a different approach based on biologically relevant sequences, we were able to determine oriC position in all 21 genomes. The position of dif, the site where chromosome dimers are resolved before DNA segregation could be predicted in 19 genomes. Computation of the core genome uncovered a number of essential gene clusters with a remarkably stable chromosomal position across species, in sharp contrast with the scrambled nature of their genomes. The active chromosomal reorganization of numerous genes acquired by horizontal transfer, mainly from mobile elements, could explain this phenomenon.
[Mh] Termos MeSH primário: Cromossomos/genética
Genes Arqueais/genética
Thermococcales/genética
[Mh] Termos MeSH secundário: Sequência de Bases
Hibridização Genômica Comparativa
Evolução Molecular
Rearranjo Gênico
Genoma
Dados de Sequência Molecular
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150714
[St] Status:MEDLINE


  4 / 29 MEDLINE  
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[PMID]:24903703
[Au] Autor:Meyer-Dombard DR; Amend JP
[Ad] Endereço:Department of Earth and Environmental Sciences, University of Illinois at Chicago, Chicago, IL, 60607, USA, drmd@uic.edu.
[Ti] Título:Geochemistry and microbial ecology in alkaline hot springs of Ambitle Island, Papua New Guinea.
[So] Source:Extremophiles;18(4):763-78, 2014 Jul.
[Is] ISSN:1433-4909
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The availability of microbiological and geochemical data from island-based and high-arsenic hydrothermal systems is limited. Here, the microbial diversity in island-based hot springs on Ambitle Island (Papua New Guinea) was investigated using culture-dependent and -independent methods. Waramung and Kapkai are alkaline springs high in sulfide and arsenic, related hydrologically to previously described hydrothermal vents in nearby Tutum Bay. Enrichments were carried out at 24 conditions with varying temperature (45, 80 °C), pH (6.5, 8.5), terminal electron acceptors (O2, SO4 (2-), S(0), NO3 (-)), and electron donors (organic carbon, H2, As(III)). Growth was observed in 20 of 72 tubes, with media targeting heterotrophic metabolisms the most successful. 16S ribosomal RNA gene surveys of environmental samples revealed representatives in 15 bacterial phyla and 8 archaeal orders. While the Kapkai 4 bacterial clone library is primarily made up of Thermodesulfobacteria (74%), no bacterial taxon represents a majority in the Kapkai 3 and Waramung samples (40% Proteobacteria and 39% Aquificae, respectively). Deinococcus/Thermus and Thermotogae are observed in all samples. The Thermococcales dominate the archaeal clone libraries (65-85%). Thermoproteales, Desulfurococcales, and uncultured Eury- and Crenarchaeota make up the remaining archaeal taxonomic diversity. The culturing and phylogenetic results are consistent with the geochemistry of the alkaline, saline, and sulfide-rich fluids. When compared to other alkaline, island-based, high-arsenic, or shallow-sea hydrothermal communities, the Ambitle Island archaeal communities are unique in geochemical conditions, and in taxonomic diversity, richness, and evenness.
[Mh] Termos MeSH primário: Deinococcus/isolamento & purificação
Fontes Termais/microbiologia
Microbiota
Proteobactérias/isolamento & purificação
Thermococcales/isolamento & purificação
[Mh] Termos MeSH secundário: Álcalis/análise
Deinococcus/genética
Fontes Termais/química
Papua Nova Guiné
Filogenia
Proteobactérias/genética
RNA Ribossômico 16S/genética
Thermococcales/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Alkalies); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1502
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM; S
[Da] Data de entrada para processamento:140607
[St] Status:MEDLINE
[do] DOI:10.1007/s00792-014-0657-6


  5 / 29 MEDLINE  
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[PMID]:24240533
[Au] Autor:Siegert M; Sitte J; Galushko A; Krüger M
[Ad] Endereço:Department of Civil and Environmental Engineering, The Pennsylvania State University, 127 Sackett Building, University Park, PA, 16802, USA, michael@siegert.org.
[Ti] Título:Starting up microbial enhanced oil recovery.
[So] Source:Adv Biochem Eng Biotechnol;142:1-94, 2014.
[Is] ISSN:0724-6145
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:This chapter gives the reader a practical introduction into microbial enhanced oil recovery (MEOR) including the microbial production of natural gas from oil. Decision makers who consider the use of one of these technologies are provided with the required scientific background as well as with practical advice for upgrading an existing laboratory in order to conduct microbiological experiments. We believe that the conversion of residual oil into natural gas (methane) and the in situ production of biosurfactants are the most promising approaches for MEOR and therefore focus on these topics. Moreover, we give an introduction to the microbiology of oilfields and demonstrate that in situ microorganisms as well as injected cultures can help displace unrecoverable oil in place (OIP). After an initial research phase, the enhanced oil recovery (EOR) manager must decide whether MEOR would be economical. MEOR generally improves oil production but the increment may not justify the investment. Therefore, we provide a brief economical assessment at the end of this chapter. We describe the necessary state-of-the-art scientific equipment to guide EOR managers towards an appropriate MEOR strategy. Because it is inevitable to characterize the microbial community of an oilfield that should be treated using MEOR techniques, we describe three complementary start-up approaches. These are: (i) culturing methods, (ii) the characterization of microbial communities and possible bio-geochemical pathways by using molecular biology methods, and (iii) interfacial tension measurements. In conclusion, we hope that this chapter will facilitate a decision on whether to launch MEOR activities. We also provide an update on relevant literature for experienced MEOR researchers and oilfield operators. Microbiologists will learn about basic principles of interface physics needed to study the impact of microorganisms living on oil droplets. Last but not least, students and technicians trying to understand processes in oilfields and the techniques to examine them will, we hope, find a valuable source of information in this review.
[Mh] Termos MeSH primário: Metano/metabolismo
Methanococcales/metabolismo
Methanomicrobiales/metabolismo
Methanosarcinales/metabolismo
Petróleo/metabolismo
Thermococcales/metabolismo
[Mh] Termos MeSH secundário: Seres Humanos
Concentração de Íons de Hidrogênio
Consórcios Microbianos/fisiologia
Gás Natural
Campos de Petróleo e Gás
Salinidade
Tensoativos/metabolismo
Temperatura Ambiente
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Natural Gas); 0 (Petroleum); 0 (Surface-Active Agents); OP0UW79H66 (Methane)
[Em] Mês de entrada:1501
[Cu] Atualização por classe:140904
[Lr] Data última revisão:
140904
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:131119
[St] Status:MEDLINE
[do] DOI:10.1007/10_2013_256


  6 / 29 MEDLINE  
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[PMID]:24391933
[Au] Autor:Butzin NC; Lapierre P; Green AG; Swithers KS; Gogarten JP; Noll KM
[Ad] Endereço:Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut, United States of America.
[Ti] Título:Reconstructed ancestral Myo-inositol-3-phosphate synthases indicate that ancestors of the Thermococcales and Thermotoga species were more thermophilic than their descendants.
[So] Source:PLoS One;8(12):e84300, 2013.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The bacterial genomes of Thermotoga species show evidence of significant interdomain horizontal gene transfer from the Archaea. Members of this genus acquired many genes from the Thermococcales, which grow at higher temperatures than Thermotoga species. In order to study the functional history of an interdomain horizontally acquired gene we used ancestral sequence reconstruction to examine the thermal characteristics of reconstructed ancestral proteins of the Thermotoga lineage and its archaeal donors. Several ancestral sequence reconstruction methods were used to determine the possible sequences of the ancestral Thermotoga and Archaea myo-inositol-3-phosphate synthase (MIPS). These sequences were predicted to be more thermostable than the extant proteins using an established sequence composition method. We verified these computational predictions by measuring the activities and thermostabilities of purified proteins from the Thermotoga and the Thermococcales species, and eight ancestral reconstructed proteins. We found that the ancestral proteins from both the archaeal donor and the Thermotoga most recent common ancestor recipient were more thermostable than their descendants. We show that there is a correlation between the thermostability of MIPS protein and the optimal growth temperature (OGT) of its host, which suggests that the OGT of the ancestors of these species of Archaea and the Thermotoga grew at higher OGTs than their descendants.
[Mh] Termos MeSH primário: Adaptação Biológica/genética
Biologia Computacional/métodos
Evolução Molecular
Temperatura Alta
Liases Intramoleculares/genética
Thermococcales/enzimologia
Thermotoga maritima/enzimologia
[Mh] Termos MeSH secundário: Archaea/enzimologia
Archaea/genética
Transferência Genética Horizontal/genética
Funções Verossimilhança
Modelos Genéticos
Filogenia
Especificidade da Espécie
Thermococcales/genética
Thermotoga maritima/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
EC 5.5.- (Intramolecular Lyases); EC 5.5.1.- (D-myo-inositol-3-phosphate synthase)
[Em] Mês de entrada:1409
[Cu] Atualização por classe:150515
[Lr] Data última revisão:
150515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140107
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0084300


  7 / 29 MEDLINE  
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[PMID]:23422322
[Au] Autor:Norais C; Moisan A; Gaspin C; Clouet-d'Orval B
[Ad] Endereço:Laboratoire de Biochimie, UMR CNRS 7654, Département de Biologie, Ecole Polytechnique, Palaiseau, France.
[Ti] Título:Diversity of CRISPR systems in the euryarchaeal Pyrococcales.
[So] Source:RNA Biol;10(5):659-70, 2013 May.
[Is] ISSN:1555-8584
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Pyrococcales are members of the order Thermococcales, a group of hyperthermophilic euryarchaea that are frequently found in deep sea hydrothermal vents. Infectious genetic elements, such as plasmids and viruses, remain a threat even in this remote environment and these microorganisms have developed several ways to fight their genetic invaders. Among these are the recently discovered CRISPR systems. In this review, we have combined and condensed available information on genetic elements infecting the Thermococcales and on the multiple CRISPR systems found in the Pyrococcales to fight them. Their organization and mode of action will be presented with emphasis on the Type III-B system that is the only CRISPR system known to target RNA molecules in a process reminiscent of RNA interference. The intriguing case of Pyrococcus abyssi, which is among the rare strains to present a CRISPR system devoid of the universal cas1 and cas2 genes, is also discussed.
[Mh] Termos MeSH primário: Sistemas CRISPR-Cas
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
Pyrococcus abyssi/genética
Pyrococcus abyssi/virologia
Thermococcales/genética
Thermococcales/virologia
[Mh] Termos MeSH secundário: Vírus de Archaea/genética
Vírus de Archaea/fisiologia
Regulação da Expressão Gênica em Archaea
Transferência Genética Horizontal
Genoma Arqueal
Filogenia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; REVIEW
[Em] Mês de entrada:1401
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130221
[St] Status:MEDLINE
[do] DOI:10.4161/rna.23927


  8 / 29 MEDLINE  
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[PMID]:23326305
[Au] Autor:Krupovic M; Gonnet M; Hania WB; Forterre P; Erauso G
[Ad] Endereço:Unité Biologie Moléculaire du Gène chez les Extrêmophiles, Département de Microbiologie, Institut Pasteur, Paris, France.
[Ti] Título:Insights into dynamics of mobile genetic elements in hyperthermophilic environments from five new Thermococcus plasmids.
[So] Source:PLoS One;8(1):e49044, 2013.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Mobilome of hyperthermophilic archaea dwelling in deep-sea hydrothermal vents is poorly characterized. To gain insight into genetic diversity and dynamics of mobile genetic elements in these environments we have sequenced five new plasmids from different Thermococcus strains that have been isolated from geographically remote hydrothermal vents. The plasmids were ascribed to two subfamilies, pTN2-like and pEXT9a-like. Gene content and phylogenetic analyses illuminated a robust connection between pTN2-like plasmids and Pyrococcus abyssi virus 1 (PAV1), with roughly half of the viral genome being composed of genes that have homologues in plasmids. Unexpectedly, pEXT9a-like plasmids were found to be closely related to the previously sequenced plasmid pMETVU01 from Methanocaldococcus vulcanius M7. Our data suggests that the latter observation is most compatible with an unprecedented horizontal transfer of a pEXT9a-like plasmid from Thermococcales to Methanococcales. Gene content analysis revealed that thermococcal plasmids encode Hfq-like proteins and toxin-antitoxin (TA) systems of two different families, VapBC and RelBE. Notably, although abundant in archaeal genomes, to our knowledge, TA and hfq-like genes have not been previously found in archaeal plasmids or viruses. Finally, the plasmids described here might prove to be useful in developing new genetic tools for hyperthermophiles.
[Mh] Termos MeSH primário: Elementos de DNA Transponíveis/genética
Fontes Hidrotermais/microbiologia
Plasmídeos/genética
Thermococcales/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas Arqueais/genética
Vírus de Archaea/genética
DNA Arqueal/química
DNA Arqueal/genética
Ordem dos Genes
Transferência Genética Horizontal
Genes Arqueais/genética
Methanococcales/classificação
Methanococcales/genética
Dados de Sequência Molecular
Filogenia
Plasmídeos/química
Plasmídeos/classificação
Pyrococcus abyssi/virologia
RNA Ribossômico 16S/genética
Origem de Replicação/genética
Análise de Sequência de DNA
Homologia de Sequência de Aminoácidos
Temperatura Ambiente
Thermococcales/classificação
Thermococcus
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Archaeal Proteins); 0 (DNA Transposable Elements); 0 (DNA, Archaeal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1307
[Cu] Atualização por classe:150219
[Lr] Data última revisão:
150219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:130118
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0049044


  9 / 29 MEDLINE  
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[PMID]:22105429
[Au] Autor:Blank CE
[Ad] Endereço:Department of Geosciences, University of Montana, 32 Campus Drive #1296, Missoula, MT 59812-1296, USA. carrine.blank@umontana.edu
[Ti] Título:An expansion of age constraints for microbial clades that lack a conventional fossil record using phylogenomic dating.
[So] Source:J Mol Evol;73(3-4):188-208, 2011 Oct.
[Is] ISSN:1432-1432
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Most microbial taxa lack a conventional microfossil or biomarker record, and so we currently have little information regarding how old most microbial clades and their associated traits are. Building on the previously published oxygen age constraint, two new age constraints are proposed based on the ability of microbial clades to metabolize chitin and aromatic compounds derived from lignin. Using the archaeal domain of life as a test case, phylogenetic analyses, along with published metabolic and genetic data, showed that members of the Halobacteriales and Thermococcales are able to metabolize chitin. Ancestral state reconstruction combined with phylogenetic analysis of the genes underlying chitin degradation predicted that the ancestors of these two groups were also likely able to metabolize chitin or chitin-related compounds. These two clades were therefore assigned a maximum age of 1.0 Ga (when chitin likely first appeared). Similar analyses also predicted that the ancestor to the Sulfolobus solfataricus-Sulfolobus islandicus clade was able to metabolize phenol using catechol dioxygenase, so this clade was assigned a maximum age of 475 Ma. Inferred ages of archaeal clades using relaxed molecular clocks with the new age constraints were consistent with those inferred with the oxygen age constraints. This work expands our current toolkit to include Paleoproterozoic, Neoproterozoic, and Paleozoic age constraints, and should aid in our ability to phylogenetically reconstruct the antiquity of a wide array of microbial clades and their associated morphological and biogeochemical traits, spanning deep geologic time. Such hypotheses-although built upon evolutionary inferences-are fundamentally testable.
[Mh] Termos MeSH primário: Halobacteriales/genética
Modelos Genéticos
Filogenia
Thermococcales/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas Arqueais/genética
Teorema de Bayes
Evolução Biológica
Quitina/metabolismo
Quitina Sintase/genética
Quitinases/genética
Simulação por Computador
Dioxigenases/genética
Especiação Genética
Halobacteriales/enzimologia
Halobacteriales/metabolismo
Lignina/metabolismo
Funções Verossimilhança
Óperon
Oxigênio/metabolismo
Análise de Sequência de Proteína
Thermococcales/enzimologia
Thermococcales/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Archaeal Proteins); 1398-61-4 (Chitin); 9005-53-2 (Lignin); EC 1.13.11.- (Dioxygenases); EC 2.4.1.16 (Chitin Synthase); EC 3.2.1.14 (Chitinases); S88TT14065 (Oxygen)
[Em] Mês de entrada:1204
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111123
[St] Status:MEDLINE
[do] DOI:10.1007/s00239-011-9467-y


  10 / 29 MEDLINE  
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[PMID]:21366819
[Au] Autor:Le Fourn C; Brasseur G; Brochier-Armanet C; Pieulle L; Brioukhanov A; Ollivier B; Dolla A
[Ad] Endereço:Laboratoire Interactions et Modulateurs de Réponses - CNRS UPR3243 - IFR88, 31 chemin Joseph Aiguier, 13402 Marseille cedex 20, France.
[Ti] Título:An oxygen reduction chain in the hyperthermophilic anaerobe Thermotoga maritima highlights horizontal gene transfer between Thermococcales and Thermotogales.
[So] Source:Environ Microbiol;13(8):2132-45, 2011 Aug.
[Is] ISSN:1462-2920
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The hyperthermophile Thermotoga maritima, although strictly anaerobic, is able to grow in the presence of low amounts of O(2). Here, we show that this bacterium consumes O(2) via a three-partner chain involving an NADH oxidoreductase (NRO), a rubredoxin (Rd) and a flavo-diiron protein (FprA) (locus tags: TM_0754, TM_0659 and TM_0755, respectively). In vitro experiments showed that the NADH-dependent O(2) consumption rate was 881.9 (± 106.7) mol O(2) consumed min(-1) per mol of FprA at 37°C and that water was the main end-product of the reaction. We propose that this O(2) reduction chain plays a central role in the O(2) tolerance of T. maritima. Phylogenetic analyses suggest that the genes coding for these three components were acquired by an ancestor of Thermotogales from an ancestor of Thermococcales via a single gene transfer. This event likely also involved two ROS scavenging enzymes (neelaredoxin and rubrerythrin) that are encoded by genes clustered with those coding for FprA, NRO and Rd in the ancestor of Thermococcales. Such genomic organization would have provided the ancestor of Thermotogales with a complete set of enzymes dedicated to O(2)-toxicity defence. Beside Thermotogales and Thermococcales, horizontal gene transfers have played a major role in disseminating these enzymes within the hyperthermophilic anaerobic prokaryotic communities, allowing them to cope with fluctuating oxidative conditions that exist in situ.
[Mh] Termos MeSH primário: Transferência Genética Horizontal
Oxigênio/metabolismo
Thermococcales/genética
Thermotoga maritima/genética
Thermotoga maritima/metabolismo
[Mh] Termos MeSH secundário: Bactérias Anaeróbias/genética
Bactérias Anaeróbias/metabolismo
Genes Bacterianos/genética
Filogenia
Thermococcales/classificação
Thermotoga maritima/classificação
Thermotoga maritima/enzimologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
S88TT14065 (Oxygen)
[Em] Mês de entrada:1207
[Cu] Atualização por classe:131121
[Lr] Data última revisão:
131121
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110304
[St] Status:MEDLINE
[do] DOI:10.1111/j.1462-2920.2011.02439.x



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