Base de dados : MEDLINE
Pesquisa : B02.500 [Categoria DeCS]
Referências encontradas : 11 [refinar]
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  1 / 11 MEDLINE  
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[PMID]:22574130
[Au] Autor:Miller-Coleman RL; Dodsworth JA; Ross CA; Shock EL; Williams AJ; Hartnett HE; McDonald AI; Havig JR; Hedlund BP
[Ad] Endereço:School of Life Sciences, University of Nevada, Las Vegas, Nevada, United States of America.
[Ti] Título:Korarchaeota diversity, biogeography, and abundance in Yellowstone and Great Basin hot springs and ecological niche modeling based on machine learning.
[So] Source:PLoS One;7(5):e35964, 2012.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Over 100 hot spring sediment samples were collected from 28 sites in 12 areas/regions, while recording as many coincident geochemical properties as feasible (>60 analytes). PCR was used to screen samples for Korarchaeota 16S rRNA genes. Over 500 Korarchaeota 16S rRNA genes were screened by RFLP analysis and 90 were sequenced, resulting in identification of novel Korarchaeota phylotypes and exclusive geographical variants. Korarchaeota diversity was low, as in other terrestrial geothermal systems, suggesting a marine origin for Korarchaeota with subsequent niche-invasion into terrestrial systems. Korarchaeota endemism is consistent with endemism of other terrestrial thermophiles and supports the existence of dispersal barriers. Korarchaeota were found predominantly in >55°C springs at pH 4.7-8.5 at concentrations up to 6.6×10(6) 16S rRNA gene copies g(-1) wet sediment. In Yellowstone National Park (YNP), Korarchaeota were most abundant in springs with a pH range of 5.7 to 7.0. High sulfate concentrations suggest these fluids are influenced by contributions from hydrothermal vapors that may be neutralized to some extent by mixing with water from deep geothermal sources or meteoric water. In the Great Basin (GB), Korarchaeota were most abundant at spring sources of pH<7.2 with high particulate C content and high alkalinity, which are likely to be buffered by the carbonic acid system. It is therefore likely that at least two different geological mechanisms in YNP and GB springs create the neutral to mildly acidic pH that is optimal for Korarchaeota. A classification support vector machine (C-SVM) trained on single analytes, two analyte combinations, or vectors from non-metric multidimensional scaling models was able to predict springs as Korarchaeota-optimal or sub-optimal habitats with accuracies up to 95%. To our knowledge, this is the most extensive analysis of the geochemical habitat of any high-level microbial taxon and the first application of a C-SVM to microbial ecology.
[Mh] Termos MeSH primário: Inteligência Artificial
Biodiversidade
Fenômenos Ecológicos e Ambientais
Fontes Termais
Korarchaeota/classificação
Filogeografia
[Mh] Termos MeSH secundário: Ecossistema
Fontes Termais/química
Concentração de Íons de Hidrogênio
Korarchaeota/genética
Temperatura Ambiente
Água/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
059QF0KO0R (Water)
[Em] Mês de entrada:1209
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:120511
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0035964


  2 / 11 MEDLINE  
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[PMID]:21965539
[Au] Autor:Das U; Smith P; Shuman S
[Ad] Endereço:Molecular Biology Program, Sloan-Kettering Institute, New York, NY 10065, USA.
[Ti] Título:Structural insights to the metal specificity of an archaeal member of the LigD 3'-phosphoesterase DNA repair enzyme family.
[So] Source:Nucleic Acids Res;40(2):828-36, 2012 Jan.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:LigD 3'-phosphoesterase (PE) enzymes perform end-healing reactions at DNA breaks. Here we characterize the 3'-ribonucleoside-resecting activity of Candidatus Korarchaeum PE. CkoPE prefers a single-stranded substrate versus a primer-template. Activity is abolished by vanadate (10 mM), but is less sensitive to phosphate (IC(50) 50 mM) or chloride (IC(50) 150 mM). The metal requirement is satisfied by manganese, cobalt, copper or cadmium, but not magnesium, calcium, nickel or zinc. Insights to CkoPE metal specificity were gained by solving new 1.5 Å crystal structures of CkoPE in complexes with Co(2+) and Zn(2+). His9, His15 and Asp17 coordinate cobalt in an octahedral complex that includes a phosphate anion, which is in turn coordinated by Arg19 and His51. The cobalt and phosphate positions and the atomic contacts in the active site are virtually identical to those in the CkoPE·Mn(2+) structure. By contrast, Zn(2+) binds in the active site in a tetrahedral complex, wherein the position, orientation and atomic contacts of the phosphate are shifted and its interaction with His51 is lost. We conclude that: (i) PE selectively binds to 'soft' metals in either productive or non-productive modes and (ii) PE catalysis depends acutely on proper metal and scissile phosphate geometry.
[Mh] Termos MeSH primário: Enzimas Reparadoras do DNA/química
Korarchaeota/enzimologia
Diester Fosfórico Hidrolases/química
[Mh] Termos MeSH secundário: Ânions
Domínio Catalítico
Cátions Bivalentes
Cobalto/química
Enzimas Reparadoras do DNA/metabolismo
Diester Fosfórico Hidrolases/metabolismo
Especificidade por Substrato
Temperatura Ambiente
Zinco/química
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Anions); 0 (Cations, Divalent); 3G0H8C9362 (Cobalt); EC 3.1.4.- (Phosphoric Diester Hydrolases); EC 6.5.1.- (DNA Repair Enzymes); J41CSQ7QDS (Zinc)
[Em] Mês de entrada:1203
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:111004
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkr767


  3 / 11 MEDLINE  
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[PMID]:21208981
[Au] Autor:Smith P; Nair PA; Das U; Zhu H; Shuman S
[Ad] Endereço:Molecular Biology Program, Sloan-Kettering Institute, New York, NY 10065, USA.
[Ti] Título:Structures and activities of archaeal members of the LigD 3'-phosphoesterase DNA repair enzyme superfamily.
[So] Source:Nucleic Acids Res;39(8):3310-20, 2011 Apr.
[Is] ISSN:1362-4962
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:LigD 3'-phosphoesterase (PE) is a component of the bacterial NHEJ apparatus that performs 3'-end-healing reactions at DNA breaks. The tertiary structure, active site and substrate specificity of bacterial PE are unique vis-à-vis other end-healing enzymes. PE homologs are present in archaea, but their properties are uncharted. Here, we demonstrate the end-healing activities of two archaeal PEs--Candidatus Korarchaeum cryptofilum PE (CkoPE; 117 amino acids) and Methanosarcina barkeri PE (MbaPE; 151 amino acids)--and we report their atomic structures at 1.1 and 2.1 Å, respectively. Archaeal PEs are minimized versions of bacterial PE, consisting of an eight-stranded ß barrel and a 3(10) helix. Their active sites are located in a crescent-shaped groove on the barrel's outer surface, wherein two histidines and an aspartate coordinate manganese in an octahedral complex that includes two waters and a phosphate anion. The phosphate is in turn coordinated by arginine and histidine side chains. The conservation of active site architecture in bacterial and archaeal PEs, and the concordant effects of active site mutations, underscore a common catalytic mechanism, entailing transition state stabilization by manganese and the phosphate-binding arginine and histidine. Our results fortify the proposal that PEs comprise a DNA repair superfamily distributed widely among taxa.
[Mh] Termos MeSH primário: Proteínas Arqueais/química
Korarchaeota/enzimologia
Methanosarcina barkeri/enzimologia
Diester Fosfórico Hidrolases/química
Monoéster Fosfórico Hidrolases/química
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas Arqueais/genética
Proteínas Arqueais/metabolismo
Biocatálise
Domínio Catalítico
Cristalografia por Raios X
Enzimas Reparadoras do DNA/química
Enzimas Reparadoras do DNA/genética
Enzimas Reparadoras do DNA/metabolismo
Modelos Moleculares
Dados de Sequência Molecular
Diester Fosfórico Hidrolases/genética
Diester Fosfórico Hidrolases/metabolismo
Monoéster Fosfórico Hidrolases/genética
Monoéster Fosfórico Hidrolases/metabolismo
Proteínas Recombinantes/química
Proteínas Recombinantes/genética
Proteínas Recombinantes/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Archaeal Proteins); 0 (Recombinant Proteins); EC 3.1.3.2 (Phosphoric Monoester Hydrolases); EC 3.1.4.- (Phosphoric Diester Hydrolases); EC 6.5.1.- (DNA Repair Enzymes)
[Em] Mês de entrada:1107
[Cu] Atualização por classe:161125
[Lr] Data última revisão:
161125
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:110107
[St] Status:MEDLINE
[do] DOI:10.1093/nar/gkq1163


  4 / 11 MEDLINE  
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[PMID]:21153671
[Au] Autor:Auchtung TA; Shyndriayeva G; Cavanaugh CM
[Ad] Endereço:Department of Organismic and Evolutionary Biology, Harvard University, Biological Laboratories 4083, 16 Divinity Avenue, Cambridge, MA 02138, USA.
[Ti] Título:16S rRNA phylogenetic analysis and quantification of Korarchaeota indigenous to the hot springs of Kamchatka, Russia.
[So] Source:Extremophiles;15(1):105-16, 2011 Jan.
[Is] ISSN:1433-4909
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The candidate archaeal division Korarchaeota is known primarily from deeply branching sequences of 16S rRNA genes PCR-amplified from hydrothermal springs. Parallels between the phylogeny of these genes and the geographic locations where they were identified suggested that Korarchaeota exhibit a high level of endemism. In this study, the influence of geographic isolation and select environmental factors on the diversification of the Korarchaeota was investigated. Fourteen hot springs from three different regions of Kamchatka, Russia were screened by PCR using Korarchaeota-specific and general Archaea 16S rRNA gene-targeting primers, cloning, and sequencing. Phylogenetic analyses of these sequences with Korarchaeota 16S rRNA sequences previously identified from around the world suggested that all Kamchatka sequences cluster together in a unique clade that subdivides by region within the peninsula. Consistent with endemism, 16S rRNA gene group-specific quantitative PCR of all Kamchatka samples detected only the single clade of Korarchaeota that was found by the non-quantitative PCR screening. In addition, their genes were measured in only low numbers; small Korarchaeota populations would present fewer chances for dispersal to and colonization of other sites. Across the entire division of Korarchaeota, common geographic locations, temperatures, or salinities of identification sites united sequence clusters at different phylogenetic levels, suggesting varied roles of these factors in the diversification of Korarchaeota.
[Mh] Termos MeSH primário: Fontes Termais/microbiologia
Korarchaeota/genética
Filogenia
RNA Ribossômico 16S/genética
Microbiologia da Água
[Mh] Termos MeSH secundário: Sibéria
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1104
[Cu] Atualização por classe:171013
[Lr] Data última revisão:
171013
[Sb] Subgrupo de revista:IM; S
[Da] Data de entrada para processamento:101215
[St] Status:MEDLINE
[do] DOI:10.1007/s00792-010-0340-5


  5 / 11 MEDLINE  
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[PMID]:19956276
[Au] Autor:Reigstad LJ; Jorgensen SL; Schleper C
[Ad] Endereço:Centre for Geobiology and Department of Biology, University of Bergen, Allégaten, Bergen, Norway. laila.reigstad@bio.uib.no
[Ti] Título:Diversity and abundance of Korarchaeota in terrestrial hot springs of Iceland and Kamchatka.
[So] Source:ISME J;4(3):346-56, 2010 Mar.
[Is] ISSN:1751-7370
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Korarchaeota constitute a recently proposed and little characterized kingdom of Archaea that might have diverged before the lineages of Crenarchaeota and Euryarchaeota split. To assess the diversity, distribution and abundance of Korarchaeota, we analysed 19 terrestrial hot springs in Hveragerdi and Krysuvik, Iceland, and in Kamchatka, Russia. The springs were 70-97 degrees C with pH 2.5-6.5. Out of 19 springs, 12 tested positive for Korarchaeota with specific primers. A Korarchaeota 16S rDNA library was made from each of these. From the 301 clones sequenced, 87 unique sequences were obtained from Iceland and 33 from Kamchatka. The similarity between Kamchatkan and Icelandic 16S rDNA sequences and that of Candidatus Korarchaeum cryptofilum was
[Mh] Termos MeSH primário: Biodiversidade
Fontes Termais/microbiologia
Korarchaeota/classificação
Korarchaeota/isolamento & purificação
[Mh] Termos MeSH secundário: Contagem de Células
Análise por Conglomerados
DNA Arqueal/química
DNA Arqueal/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Genes de RNAr
Islândia
Korarchaeota/genética
Dados de Sequência Molecular
Filogenia
RNA Arqueal/genética
RNA Ribossômico 16S/genética
Federação Russa
Análise de Sequência de DNA
Homologia de Sequência do Ácido Nucleico
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (DNA, Ribosomal); 0 (RNA, Archaeal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1004
[Cu] Atualização por classe:100217
[Lr] Data última revisão:
100217
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:091204
[St] Status:MEDLINE
[do] DOI:10.1038/ismej.2009.126


  6 / 11 MEDLINE  
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[PMID]:18577584
[Au] Autor:Nealson K
[Ad] Endereço:University of Southern California, Los Angeles, CA 90007, USA. knealson@usc.edu
[Ti] Título:A Korarchaeote yields to genome sequencing.
[So] Source:Proc Natl Acad Sci U S A;105(26):8805-6, 2008 Jul 01.
[Is] ISSN:1091-6490
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Genoma Arqueal/genética
Korarchaeota/genética
Análise de Sequência de DNA
[Mh] Termos MeSH secundário: Variação Genética
Filogenia
[Pt] Tipo de publicação:COMMENT; JOURNAL ARTICLE
[Em] Mês de entrada:0807
[Cu] Atualização por classe:140903
[Lr] Data última revisão:
140903
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080626
[St] Status:MEDLINE
[do] DOI:10.1073/pnas.0804670105


  7 / 11 MEDLINE  
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[PMID]:18535141
[Au] Autor:Elkins JG; Podar M; Graham DE; Makarova KS; Wolf Y; Randau L; Hedlund BP; Brochier-Armanet C; Kunin V; Anderson I; Lapidus A; Goltsman E; Barry K; Koonin EV; Hugenholtz P; Kyrpides N; Wanner G; Richardson P; Keller M; Stetter KO
[Ad] Endereço:Lehrstuhl für Mikrobiologie und Archaeenzentrum, Universität Regensburg, D-93053 Regensburg, Germany.
[Ti] Título:A korarchaeal genome reveals insights into the evolution of the Archaea.
[So] Source:Proc Natl Acad Sci U S A;105(23):8102-7, 2008 Jun 10.
[Is] ISSN:1091-6490
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The candidate division Korarchaeota comprises a group of uncultivated microorganisms that, by their small subunit rRNA phylogeny, may have diverged early from the major archaeal phyla Crenarchaeota and Euryarchaeota. Here, we report the initial characterization of a member of the Korarchaeota with the proposed name, "Candidatus Korarchaeum cryptofilum," which exhibits an ultrathin filamentous morphology. To investigate possible ancestral relationships between deep-branching Korarchaeota and other phyla, we used whole-genome shotgun sequencing to construct a complete composite korarchaeal genome from enriched cells. The genome was assembled into a single contig 1.59 Mb in length with a G + C content of 49%. Of the 1,617 predicted protein-coding genes, 1,382 (85%) could be assigned to a revised set of archaeal Clusters of Orthologous Groups (COGs). The predicted gene functions suggest that the organism relies on a simple mode of peptide fermentation for carbon and energy and lacks the ability to synthesize de novo purines, CoA, and several other cofactors. Phylogenetic analyses based on conserved single genes and concatenated protein sequences positioned the korarchaeote as a deep archaeal lineage with an apparent affinity to the Crenarchaeota. However, the predicted gene content revealed that several conserved cellular systems, such as cell division, DNA replication, and tRNA maturation, resemble the counterparts in the Euryarchaeota. In light of the known composition of archaeal genomes, the Korarchaeota might have retained a set of cellular features that represents the ancestral archaeal form.
[Mh] Termos MeSH primário: Evolução Biológica
Genoma Arqueal/genética
Korarchaeota/genética
[Mh] Termos MeSH secundário: Ciclo Celular
Replicação do DNA
Metabolismo Energético
Evolução Molecular
Korarchaeota/citologia
Korarchaeota/ultraestrutura
Filogenia
Biossíntese de Proteínas
Análise de Sequência de DNA
Transcrição Genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:0806
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:080607
[St] Status:MEDLINE
[do] DOI:10.1073/pnas.0801980105


  8 / 11 MEDLINE  
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[PMID]:18081935
[Au] Autor:Koonin EV; Makarova KS; Elkins JG
[Ad] Endereço:National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA. koonin@ncbi.nlm.nih.gov
[Ti] Título:Orthologs of the small RPB8 subunit of the eukaryotic RNA polymerases are conserved in hyperthermophilic Crenarchaeota and "Korarchaeota".
[So] Source:Biol Direct;2:38, 2007 Dec 14.
[Is] ISSN:1745-6150
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Although most of the key components of the transcription apparatus, and in particular, RNA polymerase (RNAP) subunits, are conserved between archaea and eukaryotes, no archaeal homologs of the small RPB8 subunit of eukaryotic RNAP have been detected. We report that orthologs of RPB8 are encoded in all sequenced genomes of hyperthermophilic Crenarchaeota and a recently sequenced "korarchaeal" genome, but not in Euryarchaeota or the mesophilic crenarchaeon Cenarchaeum symbiosum. These findings suggest that all 12 core subunits of eukaryotic RNAPs were already present in the last common ancestor of the extant archaea.
[Mh] Termos MeSH primário: Crenarchaeota/genética
RNA Polimerases Dirigidas por DNA/genética
Evolução Molecular
Genoma Arqueal
Korarchaeota/genética
[Mh] Termos MeSH secundário: Sequência de Aminoácidos
Proteínas Arqueais/genética
Células Eucarióticas
Dados de Sequência Molecular
Filogenia
Estrutura Secundária de Proteína
RNA Arqueal/genética
Homologia de Sequência de Aminoácidos
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., INTRAMURAL
[Nm] Nome de substância:
0 (Archaeal Proteins); 0 (RNA, Archaeal); EC 2.7.7.6 (DNA-Directed RNA Polymerases)
[Em] Mês de entrada:0812
[Cu] Atualização por classe:161019
[Lr] Data última revisão:
161019
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:071218
[St] Status:MEDLINE


  9 / 11 MEDLINE  
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[PMID]:16820509
[Au] Autor:Auchtung TA; Takacs-Vesbach CD; Cavanaugh CM
[Ad] Endereço:Department of Organismic and Evolutionary Biology, Harvard University, Harvard University, Cambridge, MA 02138, USA.
[Ti] Título:16S rRNA phylogenetic investigation of the candidate division "Korarchaeota".
[So] Source:Appl Environ Microbiol;72(7):5077-82, 2006 Jul.
[Is] ISSN:0099-2240
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The environmental distribution and phylogeny of "Korarchaeota," a proposed ancient archaeal division, was investigated by using the 16S rRNA gene framework. Korarchaeota-specific primers were designed based on previously published sequences and used to screen a variety of environments. Korarchaeota 16S rRNA genes were amplified exclusively from high temperature Yellowstone National Park hot springs and a 9 degrees N East Pacific Rise deep-sea hydrothermal vent. Phylogenetic analyses of these and all available sequences suggest that Korarchaeota exhibit a high level of endemicity.
[Mh] Termos MeSH primário: Fontes Termais/microbiologia
Korarchaeota/classificação
Filogenia
RNA Ribossômico 16S/genética
Água do Mar/microbiologia
[Mh] Termos MeSH secundário: DNA Arqueal/análise
Genes de RNAr
Korarchaeota/genética
Dados de Sequência Molecular
Análise de Sequência de DNA
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:0609
[Cu] Atualização por classe:170219
[Lr] Data última revisão:
170219
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:060706
[St] Status:MEDLINE


  10 / 11 MEDLINE  
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[PMID]:10741838
[Au] Autor:Reysenbach AL; Ehringer M; Hershberger K
[Ad] Endereço:Department of Biochemistry and Microbiology, Cook College, Rutgers University, New Brunswick, NJ, USA. reysenbachA@pdx.edu
[Ti] Título:Microbial diversity at 83 degrees C in Calcite Springs, Yellowstone National Park: another environment where the Aquificales and "Korarchaeota" coexist.
[So] Source:Extremophiles;4(1):61-7, 2000 Feb.
[Is] ISSN:1431-0651
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:The use of molecular phylogenetic approaches in microbial ecology has revolutionized our view of microbial diversity at high temperatures and led to the proposal of a new kingdom within the Archaea, namely, the "Korarchaeota." We report here the occurrence of another member of this archaeal group and a deeply rooted bacterial sequence from a thermal spring in Yellowstone National Park (USA). The DNA of a mixed community growing at 83 degrees C, pH 7.6, was extracted and the small subunit ribosomal RNA gene (16S rDNA) sequences were obtained using the polymerase chain reaction. The products were cloned and five different phylogenetic types ("phylotypes") were identified: four archaeal phylotypes, designated pBA1, pBA2, pBA3, and pBA5, and only one bacterial phylotype, designated pBB. pBA5 is very closely related to the korarchaeotal phylotype, pJP27, from Obsidian Pool in Yellowstone National Park. The pBB phylotype is a lineage within the Aquificales and, based on 16S rRNA sequence, is different enough from the members of the Aquificales to constitute a different genus. In situ hybridization with bacterial-specific and Aquificales-specific fluorescent oligonucleotide probes indicated the bacterial population dominated the community and most likely contributed significantly to biogeochemical cycling within the community.
[Mh] Termos MeSH primário: Archaea/classificação
Korarchaeota/classificação
[Mh] Termos MeSH secundário: Archaea/genética
Evolução Biológica
DNA Arqueal/genética
DNA Bacteriano/genética
Hibridização In Situ
Korarchaeota/genética
Microscopia de Fluorescência
Mutação
Filogenia
RNA Ribossômico 16S/genética
Análise de Sequência de DNA
Temperatura Ambiente
Wyoming
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (DNA, Archaeal); 0 (DNA, Bacterial); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:0005
[Cu] Atualização por classe:101118
[Lr] Data última revisão:
101118
[Sb] Subgrupo de revista:IM; S
[Da] Data de entrada para processamento:000331
[St] Status:MEDLINE



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