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  1 / 1317 MEDLINE  
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[PMID]:28408469
[Au] Autor:Errington J
[Ad] Endereço:Centre for Bacterial Cell Biology, Newcastle University, Newcastle-upon-Tyne NE1 7RU, U.K. jeff.errington@newcastle.ac.uk.
[Ti] Título:Cell wall-deficient, L-form bacteria in the 21st century: a personal perspective.
[So] Source:Biochem Soc Trans;45(2):287-295, 2017 Apr 15.
[Is] ISSN:1470-8752
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The peptidoglycan (PG) cell wall is a defining feature of the bacteria. It emerged very early in evolution and must have contributed significantly to the success of these organisms. The wall features prominently in our thinking about bacterial cell function, and its synthesis involves the action of several dozen proteins that are normally essential for viability. Surprisingly, it turns out to be relatively simple to generate bacterial genetic variants called L-forms that completely lack PG. They grow robustly provided that lack of the cell wall is compensated for by an osmoprotective growth medium. Although their existence has been noted and studied on and off for many decades, it is only recently that modern molecular and cellular methods have been applied to L-forms. We used as an experimental model to understand the molecular basis for the L-form switch. Key findings included the discovery that L-forms use an unusual blebbing, or tubulation and scission mechanism to proliferate. This mechanism is completely independent of the normal FtsZ-based division machinery and seems to require only an increased rate of membrane synthesis, leading to an increased surface area-to-volume ratio. Antibiotics that block cell wall precursor synthesis, such as phosphomycin, efficiently induce the L-form switch without the need for genetic change. The same antibiotics turned out to induce a similar L-form switch in a wide range of bacteria, including , in which we showed that proliferation was again FtsZ-independent. Aside from further basic science, future work on L-forms is likely to focus on their possible role in chronic or recurrent infections, their use as a model in studies of the origins of life, and possibly, biotechnological applications.
[Mh] Termos MeSH primário: Proteínas de Bactérias/metabolismo
Proteínas do Citoesqueleto/metabolismo
Fosfomicina/farmacologia
Formas L/crescimento & desenvolvimento
[Mh] Termos MeSH secundário: Bacillus subtilis/efeitos dos fármacos
Bacillus subtilis/crescimento & desenvolvimento
Bacillus subtilis/metabolismo
Escherichia coli/efeitos dos fármacos
Escherichia coli/crescimento & desenvolvimento
Escherichia coli/metabolismo
Formas L/metabolismo
Peptidoglicano/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Cytoskeletal Proteins); 0 (FtsZ protein, Bacteria); 0 (Peptidoglycan); 2N81MY12TE (Fosfomycin)
[Em] Mês de entrada:1705
[Cu] Atualização por classe:170502
[Lr] Data última revisão:
170502
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:170415
[St] Status:MEDLINE
[do] DOI:10.1042/BST20160435


  2 / 1317 MEDLINE  
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[PMID]:27294392
[Au] Autor:Markova N; Slavchev G; Djerov L; Nikolov A; Dimova T
[Ad] Endereço:a Institute of Microbiology, Bulgarian Academy of Sciences , Sofia , Bulgaria.
[Ti] Título:Mycobacterial L-forms are found in cord blood: A potential vertical transmission of BCG from vaccinated mothers.
[So] Source:Hum Vaccin Immunother;12(10):2565-2571, 2016 10 02.
[Is] ISSN:2164-554X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Our previous studies showed that mycobacterial L-forms persist in the blood of BCG vaccinated people and that BCG vaccine is able to produce, under appropriate conditions, filterable, self-replicating L-bodies with virus-like size. Because filterability is one of the characteristics of L-forms, considerable interest has been shown in their capacity to cross the maternal-fetal barrier. The current study demonstrated isolation of mycobacterial L-form cultures from umbilical cord blood of 5 healthy newborns of healthy mothers vaccinated previously with BCG. The isolated cultures showed distinctive growth characteristics of cell wall deficient L-form bacteria. Transmission electron microscopy demonstrated presence of L-bodies with extremely small size of 100 nm and revealed morphological transformations, typical for L-forms. IS6110 Real Time PCR assay confirmed that all L-form isolates were of mycobacterial origin and belonged to Mycobacterium tuberculosis complex which includes vaccinal BCG substrains. In conclusion, we could suggest that reproductive filterable L-bodies of BCG origin are able to fall in blood circulation of the fetus by vertical transmitted pathway and colonize newborns.
[Mh] Termos MeSH primário: Vacina BCG/administração & dosagem
Sangue Fetal/microbiologia
Formas L/isolamento & purificação
Mycobacterium bovis/isolamento & purificação
[Mh] Termos MeSH secundário: Feminino
Voluntários Saudáveis
Seres Humanos
Recém-Nascido
Formas L/genética
Formas L/ultraestrutura
Microscopia Eletrônica de Transmissão
Mycobacterium bovis/genética
Mycobacterium bovis/ultraestrutura
Reação em Cadeia da Polimerase em Tempo Real
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BCG Vaccine)
[Em] Mês de entrada:1710
[Cu] Atualização por classe:171112
[Lr] Data última revisão:
171112
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160614
[St] Status:MEDLINE
[do] DOI:10.1080/21645515.2016.1193658


  3 / 1317 MEDLINE  
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[PMID]:27149671
[Au] Autor:Studer P; Borisova M; Schneider A; Ayala JA; Mayer C; Schuppler M; Loessner MJ; Briers Y
[Ad] Endereço:Institute for Food, Nutrition and Health, ETH Zurich, Zurich, Switzerland.
[Ti] Título:The Absence of a Mature Cell Wall Sacculus in Stable Listeria monocytogenes L-Form Cells Is Independent of Peptidoglycan Synthesis.
[So] Source:PLoS One;11(5):e0154925, 2016.
[Is] ISSN:1932-6203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:L-forms are cell wall-deficient variants of otherwise walled bacteria that maintain the ability to survive and proliferate in absence of the surrounding peptidoglycan sacculus. While transient or unstable L-forms can revert to the walled state and may still rely on residual peptidoglycan synthesis for multiplication, stable L-forms cannot revert to the walled form and are believed to propagate in the complete absence of peptidoglycan. L-forms are increasingly studied as a fundamental biological model system for cell wall synthesis. Here, we show that a stable L-form of the intracellular pathogen Listeria monocytogenes features a surprisingly intact peptidoglycan synthesis pathway including glycosyl transfer, in spite of the accumulation of multiple mutations during prolonged passage in the cell wall-deficient state. Microscopic and biochemical analysis revealed the presence of peptidoglycan precursors and functional glycosyl transferases, resulting in the formation of peptidoglycan polymers but without the synthesis of a mature cell wall sacculus. In conclusion, we found that stable, non-reverting L-forms, which do not require active PG synthesis for proliferation, may still continue to produce aberrant peptidoglycan.
[Mh] Termos MeSH primário: Parede Celular/metabolismo
Formas L/metabolismo
Listeria monocytogenes/metabolismo
Peptidoglicano/metabolismo
[Mh] Termos MeSH secundário: Transferases/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Peptidoglycan); EC 2.- (Transferases)
[Em] Mês de entrada:1707
[Cu] Atualização por classe:170713
[Lr] Data última revisão:
170713
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160506
[St] Status:MEDLINE
[do] DOI:10.1371/journal.pone.0154925


  4 / 1317 MEDLINE  
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[PMID]:26051891
[Au] Autor:Kawai Y; Mercier R; Wu LJ; Domínguez-Cuevas P; Oshima T; Errington J
[Ad] Endereço:Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Medical School, Newcastle University, Richardson Road, Newcastle upon Tyne NE2 4AX, UK. Electronic address: y.kawai@ncl.ac.uk.
[Ti] Título:Cell growth of wall-free L-form bacteria is limited by oxidative damage.
[So] Source:Curr Biol;25(12):1613-8, 2015 Jun 15.
[Is] ISSN:1879-0445
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The peptidoglycan (PG) cell wall is a defining feature of the bacterial lineage and an important target for antibiotics, such as ß-lactams and glycopeptides. Nevertheless, many bacteria are capable of switching into a cell-wall-deficient state, called the "L-form" [1-3]. These variants have been classically identified as antibiotic-resistant forms in association with a wide range of infectious diseases [4]. L-forms become completely independent of the normally essential FtsZ cell division machinery [3, 5]. Instead, L-form proliferation is driven by a simple biophysical process based on an increased ratio of surface area to cell volume synthesis [6, 7]. We recently showed that only two genetic changes are needed for the L-form transition in Bacillus subtilis [7]. Class 1 mutations work to generate excess membrane synthesis [7]. Until now, the function of the class 2 mutations was unclear. We now show that these mutations work by counteracting an increase in the cellular levels of reactive oxygen species (ROS) originating from the electron transport pathway, which occurs in wall-deficient cells. Consistent with this, addition of a ROS scavenger or anaerobic culture conditions also worked to promote L-form growth without the class 2 mutations in both Gram-positive B. subtilis and Gram-negative Escherichia coli. Our results suggest that physiological compensation for the metabolic imbalance that occurs when cell wall synthesis is blocked is crucial for L-form proliferation in a wide range of bacteria and also provide new insights into the mode of action of antibiotics that target the bacterial cell wall.
[Mh] Termos MeSH primário: Bacillus subtilis/citologia
Parede Celular
Escherichia coli/citologia
Formas L/citologia
Estresse Oxidativo
[Mh] Termos MeSH secundário: Bacillus subtilis/genética
Transporte de Elétrons
Mutação
Oxirredução
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Em] Mês de entrada:1612
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150609
[St] Status:MEDLINE


  5 / 1317 MEDLINE  
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[PMID]:25874947
[Au] Autor:Markova N; Slavchev G; Michailova L
[Ad] Endereço:a Institute of Microbiology; Bulgarian Academy of Sciences ; Sofia , Bulgaria.
[Ti] Título:Presence of mycobacterial L-forms in human blood: Challenge of BCG vaccination.
[So] Source:Hum Vaccin Immunother;11(5):1192-200, 2015.
[Is] ISSN:2164-554X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Possible persistence of bacteria in human blood as cell wall deficient forms (L-forms) represents a top research priority for microbiologists. Application of live BCG vaccine and L-form transformation of vaccine strain may display a new intriguing aspect concerning the opportunity for occurrence of unpredictable colonization inside the human body by unusual microbial life forms. L-form cultures were isolated from 141 blood samples of people previously vaccinated with BCG, none with a history of exposure to tuberculosis. Innovative methodology to access the unusual L-form elements derived from human blood was developed. The methodology outlines the path of transformation of non- cultivable L-form element to cultivable bacteria and their adaptation for growth in vitro. All isolates showed typical L-forms growth features ("fried eggs" colonies and biofilm). Electron microscopy revealed morphology evidencing peculiar characteristics of bacterial L-form population (cell wall deficient polymorphic elements of variable shape and size). Regular detection of acid fast bacteria in smears of isolated blood L-form cultures, led us to start their identification by using specific Mycobactrium spp. genetic tests. Forty five of 97 genetically tested blood cultures provided specific positive signals for mycobacteria, confirmed by at least one of the 3 specific assays (16S rRNA PCR; IS6110 Real Time PCR and spoligotyping). In conclusion, the obtained genetic evidence suggests that these L-forms are of mycobacterial origin. As the investigated people had been vaccinated with BCG, we can assume that the identified mycobacterial L-forms may be produced by persisting live BCG vaccine.
[Mh] Termos MeSH primário: Vacina BCG/administração & dosagem
Sangue/microbiologia
Formas L/isolamento & purificação
Mycobacterium/isolamento & purificação
[Mh] Termos MeSH secundário: Adolescente
Adulto
Biofilmes/crescimento & desenvolvimento
Criança
Pré-Escolar
Elementos de DNA Transponíveis
DNA Bacteriano/química
DNA Bacteriano/genética
DNA Ribossômico/química
DNA Ribossômico/genética
Feminino
Seres Humanos
Lactente
Formas L/genética
Formas L/fisiologia
Formas L/ultraestrutura
Masculino
Microscopia Eletrônica
Meia-Idade
Tipagem Molecular
Mycobacterium/genética
Mycobacterium/fisiologia
Mycobacterium/ultraestrutura
RNA Ribossômico 16S/genética
Reação em Cadeia da Polimerase em Tempo Real
Adulto Jovem
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (BCG Vaccine); 0 (DNA Transposable Elements); 0 (DNA, Bacterial); 0 (DNA, Ribosomal); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:160415
[Lr] Data última revisão:
160415
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150416
[St] Status:MEDLINE
[do] DOI:10.1080/21645515.2015.1016682


  6 / 1317 MEDLINE  
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[PMID]:25703211
[Au] Autor:Wang DN; Wu WJ; Wang T; Pan YZ; Tang KL; She XL; Ding WJ; Wang H
[Ad] Endereço:Department of Medical Microbiology and Parasitology, Institutes of Medical Science, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
[Ti] Título:Salmonella L-forms: formation in human bile in vitro and isolation culture from patients' gallbladder samples by a non-high osmotic isolation technique.
[So] Source:Clin Microbiol Infect;21(5):470.e9-16, 2015 May.
[Is] ISSN:1469-0691
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Bacterial L-forms have always been considered as osmotic-pressure-sensitive cell-wall-deficient bacteria and isolation culture of L-forms must use media with high osmotic pressure. However, isolation culture of stable L-forms formed in humans and animals is very difficult because they have adapted to the physiological osmotic pressure condition of the host. We use a non-high osmotic isolation technique to isolate stable L-forms of Salmonella Typhi and Salmonella Paratyphi A from bile-inducer cultures in vitro and from patients' gallbladder specimens. Multiplex PCR assay for Salmonella-specific genes and nucleotide sequencing are used to identify the Salmonella L-forms in stable L-form isolates. Using this method, we confirmed that Salmonella Paratyphi A and Salmonella Typhi cannot be isolated from bile-inducer cultures cultured for 6 h or 48 h, but the L-forms can be isolated from 1 h to 45 days. In the 524 gallbladder samples, the positive rate for bacterial forms was 19.7% and the positive rate for Salmonella spp. was 0.6% by routine bacteriological methods. The positive rate for bacterial L-forms was 75.4% using non-high osmotic isolation culture. In the L-form isolates, the positive rate of Salmonella invA gene was 3.1%. In these invA-positive L-form isolates, four were positive for the invA and flic-d genes of Salmonella Typhi, and ten were positive for the invA and flic-a genes of Salmonella Paratyphi A.
[Mh] Termos MeSH primário: Técnicas Bacteriológicas/métodos
Bile/microbiologia
Vesícula Biliar/microbiologia
Formas L/isolamento & purificação
Salmonella paratyphi A/isolamento & purificação
Salmonella typhi/isolamento & purificação
[Mh] Termos MeSH secundário: Proteínas de Bactérias/genética
Meios de Cultura/química
Seres Humanos
Febre Paratifoide/microbiologia
Reação em Cadeia da Polimerase
Salmonella paratyphi A/genética
Salmonella typhi/genética
Análise de Sequência de DNA
Febre Tifoide/microbiologia
Fatores de Virulência/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Bacterial Proteins); 0 (Culture Media); 0 (Virulence Factors); 147652-43-5 (invA protein, Bacteria)
[Em] Mês de entrada:1607
[Cu] Atualização por classe:150613
[Lr] Data última revisão:
150613
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150224
[St] Status:MEDLINE


  7 / 1317 MEDLINE  
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[PMID]:25381932
[Au] Autor:Wang DN; Ding WJ; Pan YZ; Tang KL; Wang T; She XL; Wang H
[Ad] Endereço:Department of Medical Microbiology and Parasitology, Institutes of Medical Science, Shanghai Jiao Tong University School of Medicine, Shanghai, China.
[Ti] Título:The Helicobacter pylori L-form: formation and isolation in the human bile cultures in vitro and in the gallbladders of patients with biliary diseases.
[So] Source:Helicobacter;20(2):98-105, 2015 Apr.
[Is] ISSN:1523-5378
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: The Helicobacter pylori is considered the important causative agent causing biliary diseases, but the H. pylori can be isolated from very few gallbladder specimens with diseases. We studied the formation of H. pylori L-forms in bile in vitro and isolated the H. pylori L-forms from gallbladder of patients with biliary diseases. METHODS: We inoculated the H. pylori into the human bile to induce the L-form in vitro. The gallbladder specimens were collected from patients with biliary diseases to isolate the bacterial L-forms by the nonhigh osmotic isolation technique, and the H. pylori L-forms in the L-form isolates were identified by the gene assay for the H. pylori-specific genes 16S rRNA and UreA. RESULTS: The H. Pylori cannot be isolated from the bile-induced cultures, but the H. pylori L-form can be isolated from the H. pylori-negative bile-induced cultures. The L-form isolates of bile-induced cultures showed a positive reaction of the H. pylori-specific genes by PCR, and the coincidence ratio of the nucleotide sequences between the L-forms and the H. pylori is 99%. The isolation rate of bacteria L-form is 93.2% in the gallbladder specimens with bacteria-negative isolation culture by the nonhigh osmotic isolation technique, and the positive rate of the H. pylori-specific genes in the L-form isolates is 7.1% in the bacterial L-form-positive isolation cultures by the PCR. CONCLUSIONS: H. pylori can be rapidly induced into the L-form in the human bile; the L-form, as the latent bacteria, can live in the host gallbladder for a long times, and they made the host became a latent carrier of the H. pylori L-form. The H. pylori L-form can be isolated by the nonhigh osmotic isolation technique, and the variant can be identified by the gene assay for the H. pylori-specific genes 16S rRNA and reA.
[Mh] Termos MeSH primário: Bile/microbiologia
Doenças Biliares/microbiologia
Vesícula Biliar/microbiologia
Infecções por Helicobacter/microbiologia
Helicobacter pylori/isolamento & purificação
Formas L/isolamento & purificação
[Mh] Termos MeSH secundário: Portador Sadio/microbiologia
Helicobacter pylori/classificação
Helicobacter pylori/genética
Seres Humanos
Formas L/classificação
Formas L/genética
Reação em Cadeia da Polimerase
RNA Ribossômico 16S/genética
Urease/genética
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S); EC 3.5.1.5 (Urease)
[Em] Mês de entrada:1511
[Cu] Atualização por classe:150311
[Lr] Data última revisão:
150311
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141111
[St] Status:MEDLINE
[do] DOI:10.1111/hel.12181


  8 / 1317 MEDLINE  
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[PMID]:25427009
[Au] Autor:Claessen D; van Wezel GP
[Ad] Endereço:Dennis Claessen is in the Institute of Biology, Leiden University, Leiden, Netherlands.
[Ti] Título:Off the wall.
[So] Source:Elife;3, 2014 Nov 26.
[Is] ISSN:2050-084X
[Cp] País de publicação:England
[La] Idioma:eng
[Mh] Termos MeSH primário: Bactérias/citologia
Parede Celular/metabolismo
Formas L/citologia
[Pt] Tipo de publicação:COMMENT; JOURNAL ARTICLE
[Em] Mês de entrada:1507
[Cu] Atualização por classe:151028
[Lr] Data última revisão:
151028
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141127
[St] Status:MEDLINE
[do] DOI:10.7554/eLife.05427


  9 / 1317 MEDLINE  
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[PMID]:25358088
[Au] Autor:Mercier R; Kawai Y; Errington J
[Ad] Endereço:Centre for Bacterial Cell Biology, Institute for Cell and Molecular Biosciences, Newcastle University, Newcastle upon Tyne, United Kingdom.
[Ti] Título:General principles for the formation and proliferation of a wall-free (L-form) state in bacteria.
[So] Source:Elife;3, 2014 Oct 30.
[Is] ISSN:2050-084X
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The peptidoglycan cell wall is a defining structural feature of the bacterial kingdom. Curiously, some bacteria have the ability to switch to a wall-free or 'L-form' state. Although known for decades, the general properties of L-forms are poorly understood, largely due to the lack of systematic analysis of L-forms in the molecular biology era. Here we show that inhibition of peptidoglycan precursor synthesis promotes the generation of L-forms from both Gram-positive and Gram-negative bacteria. We show that the L-forms generated have in common a mechanism of proliferation involving membrane blebbing and tubulation, which is dependent on an altered rate of membrane synthesis. Crucially, this mode of proliferation is independent of the essential FtsZ based division machinery. Our results suggest that the L-form mode of proliferation is conserved across the bacterial kingdom, reinforcing the idea that it could have been used in primitive cells, and opening up its use in the generation of synthetic cells.
[Mh] Termos MeSH primário: Bactérias/citologia
Parede Celular/metabolismo
Formas L/citologia
[Mh] Termos MeSH secundário: Bactérias/crescimento & desenvolvimento
Divisão Celular
Proliferação Celular
Corynebacterium glutamicum/citologia
Escherichia coli/citologia
Ácidos Graxos/biossíntese
Peptidoglicano/metabolismo
Imagem com Lapso de Tempo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Fatty Acids); 0 (Peptidoglycan)
[Em] Mês de entrada:1507
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:141031
[St] Status:MEDLINE
[do] DOI:10.7554/eLife.04629


  10 / 1317 MEDLINE  
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[PMID]:24995493
[Au] Autor:Billings G; Ouzounov N; Ursell T; Desmarais SM; Shaevitz J; Gitai Z; Huang KC
[Ad] Endereço:Department of Physics, Stanford University, Stanford, CA, 94305, USA.
[Ti] Título:De novo morphogenesis in L-forms via geometric control of cell growth.
[So] Source:Mol Microbiol;93(5):883-96, 2014 Sep.
[Is] ISSN:1365-2958
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:In virtually all bacteria, the cell wall is crucial for mechanical integrity and for determining cell shape. Escherichia coli's rod-like shape is maintained via the spatiotemporal patterning of cell-wall synthesis by the actin homologue MreB. Here, we transiently inhibited cell-wall synthesis in E. coli to generate cell-wall-deficient, spherical L-forms, and found that they robustly reverted to a rod-like shape within several generations after inhibition cessation. The chemical composition of the cell wall remained essentially unchanged during this process, as indicated by liquid chromatography. Throughout reversion, MreB localized to inwardly curved regions of the cell, and fluorescent cell wall labelling revealed that MreB targets synthesis to those regions. When exposed to the MreB inhibitor A22, reverting cells regrew a cell wall but failed to recover a rod-like shape. Our results suggest that MreB provides the geometric measure that allows E. coli to actively establish and regulate its morphology.
[Mh] Termos MeSH primário: Escherichia coli/crescimento & desenvolvimento
Escherichia coli/genética
Formas L/crescimento & desenvolvimento
Formas L/genética
[Mh] Termos MeSH secundário: Parede Celular/genética
Parede Celular/metabolismo
Escherichia coli/metabolismo
Proteínas de Escherichia coli/genética
Proteínas de Escherichia coli/metabolismo
Regulação Bacteriana da Expressão Gênica
Formas L/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Escherichia coli Proteins); 149255-61-8 (MreB protein, E coli)
[Em] Mês de entrada:1505
[Cu] Atualização por classe:170220
[Lr] Data última revisão:
170220
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:140705
[St] Status:MEDLINE
[do] DOI:10.1111/mmi.12703



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BIREME/OPAS/OMS - Centro Latino-Americano e do Caribe de Informação em Ciências da Saúde