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[PMID]: 28456518 [Au] Autor: Medina-Colorado AA; Vincent KL; Miller AL; Maxwell CA; Dawson LN; Olive T; Kozlova EV; Baum MM; Pyles RB [Ad] Endereço: Department of Pediatrics, University of Texas Medical Branch, Galveston, TX, USA. [Ti] Título: Vaginal ecosystem modeling of growth patterns of anaerobic bacteria in microaerophilic conditions. [So] Source: Anaerobe;45:10-18, 2017 Jun. [Is] ISSN: 1095-8274 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: The human vagina constitutes a complex ecosystem created through relationships established between host mucosa and bacterial communities. In this ecosystem, classically defined bacterial aerobes and anaerobes thrive as communities in the microaerophilic environment. Levels of CO and O present in the vaginal lumen are impacted by both the ecosystem's physiology and the behavior and health of the human host. Study of such complex relationships requires controlled and reproducible causational approaches that are not possible in the human host that, until recently, was the only place these bacterial communities thrived. To address this need we have utilized our ex vivo human vaginal mucosa culture system to support controlled, reproducible colonization by vaginal bacterial communities (VBC) collected from healthy, asymptomatic donors. Parallel vaginal epithelial cells (VEC)-VBC co-cultures were exposed to two different atmospheric conditions to study the impact of CO concentrations upon the anaerobic bacteria associated with dysbiosis and inflammation. Our data suggest that in the context of transplanted VBC, increased CO favored specific lactobacilli species defined as microaerophiles when grown as monocultures. In preliminary studies, the observed community changes also led to shifts in host VEC phenotypes with significant changes in the host transcriptome, including altered expression of select molecular transporter genes. These findings support the need for additional study of the environmental changes associated with behavior and health upon the symbiotic and adversarial relationships that are formed in microbial communities present in the human vaginal ecosystem. [Mh] Termos MeSH primário: Bactérias Aeróbias/crescimento & desenvolvimento Bactérias Anaeróbias/crescimento & desenvolvimento Técnicas Bacteriológicas/métodos Ecossistema Modelos Biológicos Vagina/microbiologia [Mh] Termos MeSH secundário: Adolescente Adulto Aerobiose Anaerobiose Dióxido de Carbono/metabolismo Feminino Seres Humanos Meia-Idade Adulto Jovem [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 142M471B3J (Carbon Dioxide) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180219 [Lr] Data última revisão: 180219 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170501 [St] Status: MEDLINE

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[PMID]: 29304141 [Au] Autor: Zhang ZY; Yuan Y; Ali MW; Peng T; Peng W; Raza MF; Zhao Y; Zhang H [Ad] Endereço: College of Environmental Design, Wuhan Institute of Design and Sciences, Wuhan, Hubei, P. R. China. [Ti] Título: Cultivable anaerobic and aerobic bacterial communities in the fermentation chambers of Holotrichia parallela (coleoptera: scarabaeidae) larvae. [So] Source: PLoS One;13(1):e0190663, 2018. [Is] ISSN: 1932-6203 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: As important pests, scarab beetle larvae survive on plant biomass and the microbiota of the fermentation chamber play an important role in the digestion of lignocellulose-rich diets. However, the cultivable microbes, especially the anaerobic cultivable microbes, are still largely unknown. Here, both cultivable anaerobic and aerobic bacterial communities associated with the fermentation chamber of Holotrichia parallela larvae were investigated. In total bacteria cells directly enumerated by the 4', 6-diamidino-2-phenylindole (DAPI) staining method, the viable plate counts of cultivable bacteria in the fermentation chamber accounted for 0.92% of proportion. These cultivable bacteria were prone to attach to the fermentation chamber wall (88.41%) compared to the chamber contents. Anaerobic bacteria were dominant in the cultivable bacteria attaching to the fermentation chamber wall (70.20%), while the quantities of anaerobes and aerobes were similar in the chamber contents. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), fingerprinting and sequence analysis of isolated colonies revealed that the cultivable bacteria are affiliated with class γ-Proteobacteria, Bacteroidia, Actinobacteria, Clostridia and ß-Proteobacteria. γ-Proteobacteria was the major type of anaerobic cultivable bacteria and even the only one type of aerobic cultivable bacteria. Taken together, our results suggest, for the first time, that anaerobic microbiota are dominant in cultivable bacteria in the special anoxia niche of the fermentation chamber from H. parallela larvae. These bacterial isolates could be a treasure trove for screening lignocellulytic microbes which are essential for the plant biomass digestion of this scarab species. [Mh] Termos MeSH primário: Bactérias Aeróbias/isolamento & purificação Bactérias Anaeróbias/isolamento & purificação Coleópteros/microbiologia Fermentação Larva/microbiologia [Mh] Termos MeSH secundário: Animais Bactérias Aeróbias/genética Bactérias Aeróbias/crescimento & desenvolvimento Bactérias Anaeróbias/genética Bactérias Anaeróbias/crescimento & desenvolvimento Coleópteros/crescimento & desenvolvimento Contagem de Colônia Microbiana Eletroforese em Gel de Gradiente Desnaturante Reação em Cadeia da Polimerase RNA Ribossômico 16S/genética [Pt] Tipo de publicação: JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T [Nm] Nome de substância: 0 (RNA, Ribosomal, 16S) [Em] Mês de entrada: 1802 [Cu] Atualização por classe: 180215 [Lr] Data última revisão: 180215 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 180106 [St] Status: MEDLINE [do] DOI: 10.1371/journal.pone.0190663

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[PMID]: 28641671 [Au] Autor: Hoca S; Üstüntürk-Onan M; Ilhan-Sungur E [Ti] Título: Occurrence of Aerobic and Anaerobic Bacteria in the Consumer End of a Water Distribution System. [So] Source: Water Environ Res;89(7):598-605, 2017 Jul 01. [Is] ISSN: 1061-4303 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: In this study, mixed species biofilm formation including sulphate-reducing bacteria (SRB) on polypropylene surface and bacteriology of network water were investigated in a model water distribution system during a nine-month period. Water and biofilm samples were analyzed for the enumeration of aerobic heterotrophic bacteria (AHB), anaerobic heterotrophic bacteria (ANHB) and SRB. The number of live/dead bacteria was also analyzed by epifluorescence microscopy. In addition, extracellular polysaccharide substances (EPS) extraction, carbohydrate analysis and scanning electron microscope observation were performed. A biofilm with heterogeneous structure formed on the polypropylene surface of the model water distribution system. Live/dead staining data indicated that biofilm matured in the first month. It was observed that especially AHB entered into a viable but not culturable state because of the temperature decrease. It was also noted that temperature is an important environmental factor especially for planktonic SRB. The quantity of carbohydrate significantly decreased according to the temperature. [Mh] Termos MeSH primário: Bactérias Aeróbias/fisiologia Bactérias Anaeróbias/fisiologia Microbiologia da Água Abastecimento de Água [Mh] Termos MeSH secundário: Seres Humanos [Pt] Tipo de publicação: JOURNAL ARTICLE [Em] Mês de entrada: 1709 [Cu] Atualização por classe: 170914 [Lr] Data última revisão: 170914 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170624 [St] Status: MEDLINE [do] DOI: 10.2175/106143017X14902968254502

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[PMID]: 28628882 [Au] Autor: Chaudhari AU; Paul D; Dhotre D; Kodam KM [Ad] Endereço: Biochemistry Division, Department of Chemistry, Savitribai Phule Pune University, Pune, 411007, India. [Ti] Título: Effective biotransformation and detoxification of anthraquinone dye reactive blue 4 by using aerobic bacterial granules. [So] Source: Water Res;122:603-613, 2017 Oct 01. [Is] ISSN: 1879-2448 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Treatment of textile wastewater containing anthraquinone dye is quite a huge challenge due to its complex aromatic structure and toxicity. Present study deals with the degradation and detoxification of anthraquinone dye reactive blue 4 using aerobic bacterial granules. Bacterial granules effectively decolorized reactive blue 4 at wide range of pH (4.0-11.0) and temperature (20-55 °C) as well as decolorized and tolerated high concentration of reactive blue 4 dye upto 1000 mg l with V 6.16 ± 0.82 mg l h and K 227 ± 41 mg l . Metagenomics study evaluates important role of Clostridia, Actinobacteria, and Proteobacterial members in biotransformation and tolerance of high concentrations of reactive blue 4 dye. Up-regulation of xenobiotic degradation and environmental information processing pathways during dye exposure signifies their noteworthy role in dye degradation. Biotransformation of dye was confirmed by significant decrease in the values of total suspended solids, biological and chemical oxygen demand. The metabolites formed after biotransformation was characterized by FT-IR and GC-MS analysis. The reactive blue 4 dye was found to be phytotoxic, cytotoxic and genotoxic whereas its biotransformed product were non-toxic. This study comprehensively illustrates that, bacterial aerobic granules can be used for eco-friendly remediation and detoxification of wastewater containing high organic load of anthraquinone dye. [Mh] Termos MeSH primário: Bactérias Aeróbias/metabolismo Biotransformação Triazinas/química [Mh] Termos MeSH secundário: Antraquinonas Biodegradação Ambiental Corantes Espectroscopia de Infravermelho com Transformada de Fourier [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anthraquinones); 0 (Coloring Agents); 0 (Triazines); 13324-20-4 (procion blue MX-R) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171013 [Lr] Data última revisão: 171013 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170620 [St] Status: MEDLINE

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[PMID]: 28498940 [Au] Autor: Li Q; Song A; Peng W; Jin Z; Müller WEG; Wang X [Ad] Endereço: Institute of Karst Geology, Chinese Academy of Geological Sciences, Guilin 541004, PR China. [Ti] Título: Contribution of aerobic anoxygenic phototrophic bacteria to total organic carbon pool in aquatic system of subtropical karst catchments, Southwest China: evidence from hydrochemical and microbiological study. [So] Source: FEMS Microbiol Ecol;93(6), 2017 Jun 01. [Is] ISSN: 1574-6941 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: Aerobic anoxygenic phototrophic bacteria may play a particular role in carbon cycling of aquatic systems. However, little is known about the interaction between aerobic anoxygenic phototrophic bacteria and hydrochemistry in groundwater-surface water exchange systems of subtropical karst catchments. We carried out a detailed study on the abundance of aerobic anoxygenic phototrophic bacteria and bacterioplankton, hydrochemistry and taxonomy of bacterioplankton in the Maocun watershed, Southwest China, an area with karst geological background. Our results revealed that bacteria are the important contributors to total organic carbon source/sequestration in the groundwater-surface water of this area. The aerobic anoxygenic phototrophic bacteria, including ß-Proteobacteria, also appear in the studied water system. In addition to that, the genus Polynucleobacter of the phototropic ß-Proteobacteria shows a close link with those sampling sites by presenting bacterial origin organic carbon on CCA biplot and is found to be positively correlated with total nitrogen, dissolved oxygen and pH (r = 0.860, 0.747 and 0.813, respectively) in the Maocun watershed. The results suggest that Polynucleobacter might be involved in the production of organic carbon and might act as the negative feedback on global warming. [Mh] Termos MeSH primário: Betaproteobacteria/metabolismo Carbono/metabolismo Água Subterrânea/microbiologia Processos Fototróficos/fisiologia [Mh] Termos MeSH secundário: Organismos Aquáticos/metabolismo Bactérias Aeróbias/classificação Bactérias Aeróbias/genética Bactérias Aeróbias/metabolismo Betaproteobacteria/classificação Betaproteobacteria/genética Carbono/análise China Aquecimento Global Nitrogênio/análise Oxigênio/metabolismo RNA Ribossômico 16S/genética [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (RNA, Ribosomal, 16S); 7440-44-0 (Carbon); N762921K75 (Nitrogen); S88TT14065 (Oxygen) [Em] Mês de entrada: 1710 [Cu] Atualização por classe: 171031 [Lr] Data última revisão: 171031 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170513 [St] Status: MEDLINE [do] DOI: 10.1093/femsec/fix065

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[PMID]: 28491218 [Au] Autor: Ilechukwu GC; Ilechukwu CA; Ubesie AC; Okoroafor I; Ezeanolue BC; Ojinnaka NC [Ad] Endereço: Department of Paediatrics, Whiston Hospital, Prescot, United Kingdom. [Ti] Título: Bacterial agents of the discharging middle ear among children seen at the University of Nigeria Teaching Hospital, Enugu. [So] Source: Pan Afr Med J;26:87, 2017. [Is] ISSN: 1937-8688 [Cp] País de publicação: Uganda [La] Idioma: eng [Ab] Resumo: INTRODUCTION: Discharging middle ear continues to be one of the commonest problems seen in the developing world. There is an ever growing need to carry out studies periodically to determine the common bacterial agents responsible for discharging otitis media and their antibiotic sensitivity especially in set-ups characterized with minimal laboratory services. The study sought to determine the common bacterial agents causing discharging middle ear among children presenting at the University of Nigeria Teaching Hospital, Enugu and their sensitivity to the commonly available antibiotics. METHODS: Middle ear swabs were collected from 100 children aged 1 month to 17 years at the Children Out-Patient and Otorhinolaryngology Clinics of the University of Nigeria Teaching Hospital, Enugu, Nigeria. The specimens were cultured for aerobic bacterial organisms and their sensitivity determined. RESULTS: Among those with acute discharge, was isolated in 31.3% and Proteus species in 25.0%. In chronically discharging ears, Proteus Species dominated (39.1%), followed by (28.3%). CONCLUSION: and Proteus species were the commonest bacterial agents in acute and chronic otitis media respectively. Most isolates showed high sensitivity to the fluoroquinolone antibiotics. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Bactérias Aeróbias/efeitos dos fármacos Infecções Bacterianas/microbiologia Otite Média Supurativa/microbiologia [Mh] Termos MeSH secundário: Doença Aguda Adolescente Bactérias Aeróbias/isolamento & purificação Criança Pré-Escolar Doença Crônica Feminino Hospitais de Ensino Seres Humanos Lactente Masculino Testes de Sensibilidade Microbiana Nigéria Projetos Piloto [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170613 [Lr] Data última revisão: 170613 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170512 [St] Status: MEDLINE [do] DOI: 10.11604/pamj.2017.26.87.9243

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[PMID]: 28378313 [Au] Autor: Dolinová I; Strojsová M; Cerník M; Nemecek J; Machácková J; Sevcu A [Ad] Endereço: Institute for Nanomaterials, Advanced Technologies and Innovation, Technical University of Liberec, Studentská 2, 461 17, Liberec, Czech Republic. [Ti] Título: Microbial degradation of chloroethenes: a review. [So] Source: Environ Sci Pollut Res Int;24(15):13262-13283, 2017 May. [Is] ISSN: 1614-7499 [Cp] País de publicação: Germany [La] Idioma: eng [Ab] Resumo: Contamination by chloroethenes has a severe negative effect on both the environment and human health. This has prompted intensive remediation activity in recent years, along with research into the efficacy of natural microbial communities for degrading toxic chloroethenes into less harmful compounds. Microbial degradation of chloroethenes can take place either through anaerobic organohalide respiration, where chloroethenes serve as electron acceptors; anaerobic and aerobic metabolic degradation, where chloroethenes are used as electron donors; or anaerobic and aerobic co-metabolic degradation, with chloroethene degradation occurring as a by-product during microbial metabolism of other growth substrates, without energy or carbon benefit. Recent research has focused on optimising these natural processes to serve as effective bioremediation technologies, with particular emphasis on (a) the diversity and role of bacterial groups involved in dechlorination microbial processes, and (b) detection of bacterial enzymes and genes connected with dehalogenation activity. In this review, we summarise the different mechanisms of chloroethene bacterial degradation suitable for bioremediation and provide a list of dechlorinating bacteria. We also provide an up-to-date summary of primers available for detecting functional genes in anaerobic and aerobic bacteria degrading chloroethenes metabolically or co-metabolically. [Mh] Termos MeSH primário: Biodegradação Ambiental Cloreto de Vinil/metabolismo [Mh] Termos MeSH secundário: Bactérias/metabolismo Bactérias Aeróbias/metabolismo Halogenação Tetracloroetileno/metabolismo [Pt] Tipo de publicação: JOURNAL ARTICLE; REVIEW [Nm] Nome de substância: TJ904HH8SN (Tetrachloroethylene); WD06X94M2D (Vinyl Chloride) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 171104 [Lr] Data última revisão: 171104 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170406 [St] Status: MEDLINE [do] DOI: 10.1007/s11356-017-8867-y

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[PMID]: 28267865 [Au] Autor: An KA; Arshad MS; Jo Y; Chung N; Kwon JH [Ad] Endereço: Hazardous Substances Analysis Div., Daegu Regional Food and Drug Administration, Daegu, 704-940, Korea. [Ti] Título: E-Beam Irradiation for Improving the Microbiological Quality of Smoked Duck Meat with Minimum Effects on Physicochemical Properties During Storage. [So] Source: J Food Sci;82(4):865-872, 2017 Apr. [Is] ISSN: 1750-3841 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: This study was performed to evaluate the effect of different doses (0, 1.5, 3, and 4.5 kGy) of e-beam irradiation on the quality parameters (pH, Hunter's parameter, and heme pigment) and stability qualifiers (peroxide value [POV], thiobarbituric acid reactive substances [TBARSs], and total volatile basic nitrogen [TVBN]) of smoked duck meat during 40 d of storage under vacuum packaging at 4 °C. The initial populations of total bacteria (7.81 log CFU/g) and coliforms (5.68 log CFU/g) were reduced by approximately 2 to 5 log cycles with respect to irradiation doses. The results showed that pH, myoglobin, met-myoglobin, L , a , and b showed significant differences with respect to different doses and storage intervals; a and b did not vary significantly because of storage. Higher pH was found in samples treated with 4.5 kGy at 40 d, while the minimum was observed in nonirradiated samples at day 0 of storage. Higher POV (2.31 ± 0.03 meq peroxide/kg) and TBARS (5.24 ± 0.03 mg MDA/kg) values were found in 4.5 kGy-treated smoked meat at 40 d and the lowest was reported in 0 kGy-treated meat at initiation of storage (0 d). However, irradiation suppressed TVBN during storage and higher TVBN (7.09 ± 0.32 mg/100 mL) was found in duck meat treated with 0 kGy at 40 d. The electronic nose (e-nose) effectively distinguished flavor profiles during the different storage intervals. The results showed that different sensory attributes did not vary significantly with respect to the dose of irradiation. We conclude that low dose of e-beam irradiation and vacuum packaging is beneficial for safety and shelf life extension without affecting the sensory characteristics of smoked duck meat. [Mh] Termos MeSH primário: Irradiação de Alimentos Armazenamento de Alimentos Produtos da Carne/microbiologia Produtos da Carne/efeitos da radiação [Mh] Termos MeSH secundário: Animais Bactérias Aeróbias/isolamento & purificação Bactérias Aeróbias/efeitos da radiação Temperatura Baixa Contagem de Colônia Microbiana Cor Comportamento do Consumidor Patos Análise de Alimentos Contaminação de Alimentos Microbiologia de Alimentos Embalagem de Alimentos Seres Humanos Concentração de Íons de Hidrogênio Nitrogênio/análise Paladar Substâncias Reativas com Ácido Tiobarbitúrico/análise Vácuo Compostos Orgânicos Voláteis/análise [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Thiobarbituric Acid Reactive Substances); 0 (Volatile Organic Compounds); N762921K75 (Nitrogen) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170609 [Lr] Data última revisão: 170609 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170308 [St] Status: MEDLINE [do] DOI: 10.1111/1750-3841.13671

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[PMID]: 28264656 [Au] Autor: Yang Q; Zhang H; Wang Y; Xu Z; Zhang G; Chen X; Xu Y; Cao B; Kong H; Ni Y; Yu Y; Sun Z; Hu B; Huang W; Wang Y; Wu A; Feng X; Liao K; Luo Y; Hu Z; Chu Y; Lu J; Su J; Gui B; Duan Q; Zhang S; Shao H; Badal RE [Ad] Endereço: Department of Clinical Laboratory, Peking Union Medical College Hospital, Peking Union Medical College, Chinese Academy of Medical Sciences, Beijing, 100730, China. [Ti] Título: Antimicrobial susceptibilities of aerobic and facultative gram-negative bacilli isolated from Chinese patients with urinary tract infections between 2010 and 2014. [So] Source: BMC Infect Dis;17(1):192, 2017 Mar 06. [Is] ISSN: 1471-2334 [Cp] País de publicação: England [La] Idioma: eng [Ab] Resumo: BACKGROUND: The objective of this study was to investigate the distribution and susceptibility of aerobic and facultative Gram-negative bacilli isolated from Chinese patients with UTIs collected within 48 h (community acquired, CA) or after 48 h (hospital acquired, HA) of hospital admission. METHODS: From 2010 to 2014, the minimum inhibitory concentrations (MICs) of 12 antibiotics for 4,332 aerobic and facultative Gram-negative bacilli, sampled in 21 hospitals in 16 cities, were determined by the broth microdilution method. RESULTS: Enterobacteriaceae composed 88.5% of the total isolates, with Escherichia coli (E. coli) (63.2%) the most commonly isolated species, followed by Klebsiella pneumoniae (K. pneumoniae) (12.2%). Non-Enterobacteriaceae accounted for only 11.5% of all isolates and included mainly Pseudomonas aeruginosa (P. aeruginosa) (6.9%) and Acinetobacter baumannii (A. baumannii) (3.3%). Among the antimicrobial agents tested, the susceptibility rates of E.coli to the two carbapenems, ertapenem and imipenem as well as amikacin and piperacillin-tazobactam ranged from 92.5 to 98.7%. Against K. pneumonia, the most potent antibiotics were imipenem (92.6% susceptibility), amikacin (89.2% susceptibility) and ertapenem (87.9% susceptibility). Although non-Enterobacteriaceae did not show high susceptibilities to the 12 common antibiotics, amikacin exhibited the highest in vitro activity against P. aeruginosa over the 5-year study period, followed by piperacillin-tazobactam, imipenem, ceftazidime, cefepime, ciprofloxacin, and levofloxacin. The Extended Spectrum Beta-Lactamase (ESBL) rates decreased slowly during the 5 years in E. coli from 68.6% in 2010 to 59.1% in 2014, in K. pneumoniae from 59.7 to 49.2%, and in Proteus mirabilis (P. mirabilis) from 40.0 to 26.1%. However, the ESBL rates were different in 5 regions of China (Northeast, North, East, South and Middle-China). CONCLUSION: E. coli and K. pneumonia were the major pathogens causing UTIs and carbapenems and amikacin retained the highest susceptibility rates over the 5-year study period, indicating that they are good drug choices for empirical therapies, particularly of CA UTIs in China. [Mh] Termos MeSH primário: Antibacterianos/farmacologia Bactérias Aeróbias/efeitos dos fármacos Farmacorresistência Bacteriana Bactérias Gram-Negativas/efeitos dos fármacos Infecções por Bactérias Gram-Negativas/microbiologia Infecções Urinárias/microbiologia [Mh] Termos MeSH secundário: Bactérias Aeróbias/isolamento & purificação China Bactérias Gram-Negativas/isolamento & purificação Infecções por Bactérias Gram-Negativas/diagnóstico Seres Humanos Testes de Sensibilidade Microbiana Infecções Urinárias/diagnóstico [Pt] Tipo de publicação: JOURNAL ARTICLE [Nm] Nome de substância: 0 (Anti-Bacterial Agents) [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170630 [Lr] Data última revisão: 170630 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170308 [St] Status: MEDLINE [do] DOI: 10.1186/s12879-017-2296-x

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[PMID]: 28094842 [Au] Autor: Cobo T; Vives I; Rodríguez-Trujillo A; Murillo C; Ángeles MA; Bosch J; Vergara A; Gratacós E; Palacio M [Ad] Endereço: BCNatal - Barcelona Center for Maternal-Fetal and Neonatal Medicine, Hospital Clínic and Hospital Sant Joan de Deu, Clinical Institute of Gynecology, Obstetrics and Neonatology, Fetal i+D Fetal Medicine Research Center, Biomedical Research Institute August Pi I Sunyer (IDIBAPS), University of Barcel [Ti] Título: Impact of microbial invasion of amniotic cavity and the type of microorganisms on short-term neonatal outcome in women with preterm labor and intact membranes. [So] Source: Acta Obstet Gynecol Scand;96(5):570-579, 2017 May. [Is] ISSN: 1600-0412 [Cp] País de publicação: United States [La] Idioma: eng [Ab] Resumo: INTRODUCTION: The objective of this study was to evaluate the impact of microbial invasion of the amniotic cavity and the type of microorganisms on pregnancy and short-term neonatal outcomes in women with preterm labor. MATERIAL AND METHODS: Prospective observational cohort study including women with preterm labor from 22.0 to 36.0 weeks. Microbial invasion of the amniotic cavity was defined based on amniotic fluid aerobic/anaerobic/mycoplasma cultures, and intra-amniotic inflammation on amniotic fluid interleukin-6 levels. Demographic data and pregnancy outcomes were compared among women exposed to microbial invasion of the amniotic cavity by Ureaplasma spp., women with microbial invasion of the amniotic cavity by other microorganisms, and a No-microbial invasion of the amniotic cavity/No-intra-amniotic inflammation group. The short-term neonatal outcome was evaluated in women delivering after 24.0 weeks. RESULTS: We included 228 women with preterm labor. Microbial invasion of the amniotic cavity occurred in 35% (80/228), 28% (22/80) being caused by Ureaplasma spp. Gestational age at admission and at delivery were significantly earlier and the rate of delivery at <24.0 weeks' gestation and of women who further developed clinical chorioamnionitis were significantly higher in women with microbial invasion of the amniotic cavity by microorganisms other than Ureaplasma spp. However, after 24 weeks, regardless of the microorganisms isolated, the short-term neonatal outcome was similar between women exposed to microbial invasion of the amniotic cavity and the No-microbial invasion of the amniotic cavity/No-intra-amniotic inflammation group when gestational age was considered. CONCLUSIONS: Microbial invasion of the amniotic cavity by microorganisms other than Ureaplasma spp. was associated with earlier gestational age at admission and at delivery, and a higher rate of preterm delivery <24.0 weeks and of women who developed clinical chorioamnionitis. However, we did not find differences in the short-term neonatal outcome between women exposed to microbial invasion of the amniotic cavity and the no-microbial invasion of the amniotic cavity/no-intra-amniotic inflammation group delivering after 24.0 weeks' gestation when adjusted by gestational age at delivery. [Mh] Termos MeSH primário: Líquido Amniótico/microbiologia Corioamnionite/microbiologia Trabalho de Parto Prematuro [Mh] Termos MeSH secundário: Adulto Bactérias Aeróbias/isolamento & purificação Bactérias Anaeróbias/isolamento & purificação Estudos de Coortes Feminino Idade Gestacional Seres Humanos Recém-Nascido Gravidez Resultado da Gravidez Estudos Prospectivos [Pt] Tipo de publicação: JOURNAL ARTICLE; OBSERVATIONAL STUDY [Em] Mês de entrada: 1706 [Cu] Atualização por classe: 170623 [Lr] Data última revisão: 170623 [Sb] Subgrupo de revista: IM [Da] Data de entrada para processamento: 170118 [St] Status: MEDLINE [do] DOI: 10.1111/aogs.13095

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1			PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
2	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2
3	and or and not		PalavrasDescritor de assuntoDescritor de assunto primárioLimitesAutorPalavras do títuloPalavras do resumoIdiomaPaís de publicaçãoNome de substânciaNome de pessoa como assuntoRevistaAssunto de RevistaISSNTipo de publicaçãoIdentificador únicoMês de entradaAno de publicaçãoTexto completoAtualização por classeCategoria DeCSCategoria DeCS explodidaCategoria CID-10SubSetsPais de AfiliaçãoAfiliaçãoAfiliação 2

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