Base de dados : MEDLINE
Pesquisa : B03.140.094 [Categoria DeCS]
Referências encontradas : 427 [refinar]
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  1 / 427 MEDLINE  
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[PMID]:27885053
[Au] Autor:Johnson JS; Opiyo MN; Thomson M; Gharbi K; Seckl JR; Heger A; Chapman KE
[Ad] Endereço:Computational Genomics Analysis and TrainingMedical Research Council-Functional Genomics Unit, Department of Physiology, Anatomy and Genetics, University of Oxford, Oxford, UK.
[Ti] Título:11ß-hydroxysteroid dehydrogenase-1 deficiency alters the gut microbiome response to Western diet.
[So] Source:J Endocrinol;232(2):273-283, 2017 Feb.
[Is] ISSN:1479-6805
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The enzyme 11ß-hydroxysteroid dehydrogenase (11ß-HSD) interconverts active glucocorticoids and their intrinsically inert 11-keto forms. The type 1 isozyme, 11ß-HSD1, predominantly reactivates glucocorticoids in vivo and can also metabolise bile acids. 11ß-HSD1-deficient mice show altered inflammatory responses and are protected against the adverse metabolic effects of a high-fat diet. However, the impact of 11ß-HSD1 on the composition of the gut microbiome has not previously been investigated. We used high-throughput 16S rDNA amplicon sequencing to characterise the gut microbiome of 11ß-HSD1-deficient and C57Bl/6 control mice, fed either a standard chow diet or a cholesterol- and fat-enriched 'Western' diet. 11ß-HSD1 deficiency significantly altered the composition of the gut microbiome, and did so in a diet-specific manner. On a Western diet, 11ß-HSD1 deficiency increased the relative abundance of the family Bacteroidaceae, and on a chow diet, it altered relative abundance of the family Prevotellaceae Our results demonstrate that (i) genetic effects on host-microbiome interactions can depend upon diet and (ii) that alterations in the composition of the gut microbiome may contribute to the aspects of the metabolic and/or inflammatory phenotype observed with 11ß-HSD1 deficiency.
[Mh] Termos MeSH primário: 11-beta-Hidroxiesteroide Desidrogenase Tipo 1/metabolismo
Bacteroidaceae/isolamento & purificação
Ceco/microbiologia
Colo/microbiologia
Dieta Ocidental
Microbioma Gastrointestinal/genética
[Mh] Termos MeSH secundário: 11-beta-Hidroxiesteroide Desidrogenase Tipo 1/genética
Animais
Ceco/metabolismo
Colo/metabolismo
Camundongos
Camundongos Knockout
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenase Type 1)
[Em] Mês de entrada:1706
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:161126
[St] Status:MEDLINE


  2 / 427 MEDLINE  
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[PMID]:27633926
[Au] Autor:Miceli JF; Torres CI; Krajmalnik-Brown R
[Ad] Endereço:Swette Center for Environmental Biotechnology, Arizona State University, 1001 S. McAllister Ave, Tempe 85287, Arizona, USA.
[Ti] Título:Shifting the balance of fermentation products between hydrogen and volatile fatty acids: microbial community structure and function.
[So] Source:FEMS Microbiol Ecol;92(12), 2016 Dec.
[Is] ISSN:1574-6941
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:Fermentation is a key process in many anaerobic environments. Varying the concentration of electron donor fed to a fermenting community is known to shift the distribution of products between hydrogen, fatty acids and alcohols. Work to date has focused mainly on the fermentation of glucose, and how the microbial community structure is affected has not been explored. We fed ethanol, lactate, glucose, sucrose or molasses at 100 me- eq. L , 200 me- eq. L or 400 me- eq. L to batch-fed cultures with fermenting, methanogenic communities. In communities fed high concentrations of electron donor, the fraction of electrons channeled to methane decreased, from 34% to 6%, while the fraction of electrons channeled to short chain fatty acids increased, from 52% to 82%, averaged across all electron donors. Ethanol-fed cultures did not produce propionate, but did show an increase in electrons directed to acetate as initial ethanol concentration increased. In glucose, sucrose, molasses and lactate-fed cultures, propionate accumulation co-occurred with known propionate producing organisms. Overall, microbial communities were determined by the substrate provided, rather than its initial concentration, indicating that a change in community function, rather than community structure, is responsible for shifts in the fermentation products produced.
[Mh] Termos MeSH primário: Bacteroidaceae/metabolismo
Clostridiales/metabolismo
Ácidos Graxos Voláteis/biossíntese
Fermentação/fisiologia
Metano/biossíntese
Oxigênio/metabolismo
[Mh] Termos MeSH secundário: Acetatos/metabolismo
Anaerobiose/fisiologia
Reatores Biológicos
Etanol/química
Glucose/metabolismo
Hidrogênio/química
Concentração de Íons de Hidrogênio
Melaço
Propionatos/metabolismo
Sacarose/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE
[Nm] Nome de substância:
0 (Acetates); 0 (Fatty Acids, Volatile); 0 (Propionates); 3K9958V90M (Ethanol); 57-50-1 (Sucrose); 7YNJ3PO35Z (Hydrogen); IY9XDZ35W2 (Glucose); OP0UW79H66 (Methane); S88TT14065 (Oxygen)
[Em] Mês de entrada:1708
[Cu] Atualização por classe:170803
[Lr] Data última revisão:
170803
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160917
[St] Status:MEDLINE


  3 / 427 MEDLINE  
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[PMID]:27463672
[Au] Autor:Moeller AH; Caro-Quintero A; Mjungu D; Georgiev AV; Lonsdorf EV; Muller MN; Pusey AE; Peeters M; Hahn BH; Ochman H
[Ad] Endereço:Department of Integrative Biology, 2506 Speedway A5000, University of Texas at Austin, Austin, TX 78712, USA. Miller Institute for Basic Research in Science, 2536 Channing Way, University of California, Berkeley, CA 94720, USA.
[Ti] Título:Cospeciation of gut microbiota with hominids.
[So] Source:Science;353(6297):380-2, 2016 Jul 22.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:The evolutionary origins of the bacterial lineages that populate the human gut are unknown. Here we show that multiple lineages of the predominant bacterial taxa in the gut arose via cospeciation with humans, chimpanzees, bonobos, and gorillas over the past 15 million years. Analyses of strain-level bacterial diversity within hominid gut microbiomes revealed that clades of Bacteroidaceae and Bifidobacteriaceae have been maintained exclusively within host lineages across hundreds of thousands of host generations. Divergence times of these cospeciating gut bacteria are congruent with those of hominids, indicating that nuclear, mitochondrial, and gut bacterial genomes diversified in concert during hominid evolution. This study identifies human gut bacteria descended from ancient symbionts that speciated simultaneously with humans and the African apes.
[Mh] Termos MeSH primário: Actinobacteria/classificação
Bacteroidaceae/classificação
Evolução Biológica
Microbioma Gastrointestinal/fisiologia
Hominidae/microbiologia
[Mh] Termos MeSH secundário: Actinobacteria/genética
Actinobacteria/fisiologia
Animais
Bacteroidaceae/genética
Bacteroidaceae/fisiologia
Núcleo Celular
Microbioma Gastrointestinal/genética
Genoma Bacteriano
Genoma Mitocondrial
Seres Humanos
Filogenia
Especificidade da Espécie
Simbiose
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Em] Mês de entrada:1608
[Cu] Atualização por classe:170414
[Lr] Data última revisão:
170414
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160728
[St] Status:MEDLINE
[do] DOI:10.1126/science.aaf3951


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[PMID]:27463659
[Au] Autor:Segre JA; Salafsky N
[Ad] Endereço:Microbial Genomics Section, Translational and Functional Genomics Branch, National Human Genome Research Institute, Bethesda, MD 20892, USA. jsegre@nhgri.nih.gov.
[Ti] Título:EVOLUTION. Hominid superorganisms.
[So] Source:Science;353(6297):350-1, 2016 Jul 22.
[Is] ISSN:1095-9203
[Cp] País de publicação:United States
[La] Idioma:eng
[Mh] Termos MeSH primário: Actinobacteria/classificação
Bacteroidaceae/classificação
Evolução Biológica
Microbioma Gastrointestinal/fisiologia
Hominidae/microbiologia
[Mh] Termos MeSH secundário: Animais
Seres Humanos
[Pt] Tipo de publicação:COMMENT; JOURNAL ARTICLE
[Em] Mês de entrada:1608
[Cu] Atualização por classe:160728
[Lr] Data última revisão:
160728
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160728
[St] Status:MEDLINE
[do] DOI:10.1126/science.aag2788


  5 / 427 MEDLINE  
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[PMID]:26940874
[Au] Autor:McLuskey K; Grewal JS; Das D; Godzik A; Lesley SA; Deacon AM; Coombs GH; Elsliger MA; Wilson IA; Mottram JC
[Ad] Endereço:From the Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8TA, United Kingdom.
[Ti] Título:Crystal Structure and Activity Studies of the C11 Cysteine Peptidase from Parabacteroides merdae in the Human Gut Microbiome.
[So] Source:J Biol Chem;291(18):9482-91, 2016 Apr 29.
[Is] ISSN:1083-351X
[Cp] País de publicação:United States
[La] Idioma:eng
[Ab] Resumo:Clan CD cysteine peptidases, a structurally related group of peptidases that include mammalian caspases, exhibit a wide range of important functions, along with a variety of specificities and activation mechanisms. However, for the clostripain family (denoted C11), little is currently known. Here, we describe the first crystal structure of a C11 protein from the human gut bacterium, Parabacteroides merdae (PmC11), determined to 1.7-Å resolution. PmC11 is a monomeric cysteine peptidase that comprises an extended caspase-like α/ß/α sandwich and an unusual C-terminal domain. It shares core structural elements with clan CD cysteine peptidases but otherwise structurally differs from the other families in the clan. These studies also revealed a well ordered break in the polypeptide chain at Lys(147), resulting in a large conformational rearrangement close to the active site. Biochemical and kinetic analysis revealed Lys(147) to be an intramolecular processing site at which cleavage is required for full activation of the enzyme, suggesting an autoinhibitory mechanism for self-preservation. PmC11 has an acidic binding pocket and a preference for basic substrates, and accepts substrates with Arg and Lys in P1 and does not require Ca(2+) for activity. Collectively, these data provide insights into the mechanism and activity of PmC11 and a detailed framework for studies on C11 peptidases from other phylogenetic kingdoms.
[Mh] Termos MeSH primário: Proteínas de Bactérias/química
Bacteroidaceae/enzimologia
Cisteína Proteases/química
Microbioma Gastrointestinal
[Mh] Termos MeSH secundário: Cristalografia por Raios X
Seres Humanos
Estrutura Secundária de Proteína
Estrutura Terciária de Proteína
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T; RESEARCH SUPPORT, U.S. GOV'T, NON-P.H.S.
[Nm] Nome de substância:
0 (Bacterial Proteins); EC 3.4.- (Cysteine Proteases)
[Em] Mês de entrada:1611
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160305
[St] Status:MEDLINE
[do] DOI:10.1074/jbc.M115.706143


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[PMID]:26837003
[Au] Autor:Dubin K; Callahan MK; Ren B; Khanin R; Viale A; Ling L; No D; Gobourne A; Littmann E; Huttenhower C; Pamer EG; Wolchok JD
[Ad] Endereço:Infectious Diseases Service, Department of Medicine, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA.
[Ti] Título:Intestinal microbiome analyses identify melanoma patients at risk for checkpoint-blockade-induced colitis.
[So] Source:Nat Commun;7:10391, 2016 Feb 02.
[Is] ISSN:2041-1723
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:The composition of the intestinal microbiota influences the development of inflammatory disorders. However, associating inflammatory diseases with specific microbial members of the microbiota is challenging, because clinically detectable inflammation and its treatment can alter the microbiota's composition. Immunologic checkpoint blockade with ipilimumab, a monoclonal antibody that blocks cytotoxic T-lymphocyte-associated antigen-4 (CTLA-4) signalling, is associated with new-onset, immune-mediated colitis. Here we conduct a prospective study of patients with metastatic melanoma undergoing ipilimumab treatment and correlate the pre-inflammation faecal microbiota and microbiome composition with subsequent colitis development. We demonstrate that increased representation of bacteria belonging to the Bacteroidetes phylum is correlated with resistance to the development of checkpoint-blockade-induced colitis. Furthermore, a paucity of genetic pathways involved in polyamine transport and B vitamin biosynthesis is associated with an increased risk of colitis. Identification of these biomarkers may enable interventions to reduce the risk of inflammatory complications following cancer immunotherapy.
[Mh] Termos MeSH primário: Anticorpos Monoclonais/efeitos adversos
Colite/microbiologia
Microbioma Gastrointestinal/genética
Melanoma/tratamento farmacológico
RNA Ribossômico 16S/genética
Neoplasias Cutâneas/tratamento farmacológico
[Mh] Termos MeSH secundário: Adulto
Idoso
Idoso de 80 Anos ou mais
Bacteroidaceae
Bacteroidetes/genética
Bacteroidetes/isolamento & purificação
Colite/induzido quimicamente
Feminino
Seres Humanos
Ipilimumab
Masculino
Melanoma/secundário
Meia-Idade
Estudos Prospectivos
Fatores de Risco
Análise de Sequência de RNA
Neoplasias Cutâneas/patologia
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, N.I.H., EXTRAMURAL; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antibodies, Monoclonal); 0 (Ipilimumab); 0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1606
[Cu] Atualização por classe:171116
[Lr] Data última revisão:
171116
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:160203
[St] Status:MEDLINE
[do] DOI:10.1038/ncomms10391


  7 / 427 MEDLINE  
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[PMID]:25985709
[Au] Autor:Melli LC; do Carmo-Rodrigues MS; Araújo-Filho HB; Solé D; de Morais MB
[Ad] Endereço:Division of Pediatric Gastroenterology, Department of Pediatrics, Federal University of São Paulo/UNIFESP, São Paulo, Brazil; Biological and Health Sciences Department, University Center UNIFIEO, Osasco, São Paulo, Brazil.
[Ti] Título:Intestinal microbiota and allergic diseases: A systematic review.
[So] Source:Allergol Immunopathol (Madr);44(2):177-88, 2016 Mar-Apr.
[Is] ISSN:1578-1267
[Cp] País de publicação:Spain
[La] Idioma:eng
[Ab] Resumo:Evidence suggests that possible imbalances in intestinal microbiota composition may be implicated in the occurrence of allergic diseases. Although several studies published until 2006 indicated a correlation between microbiota composition and allergic symptoms, it has not been possible to distinguish protective microorganisms from those associated with increased risk of allergic diseases. Therefore, the objective of this study was to review the studies published since 2007 that address the intestinal microbiota in allergic diseases. Twenty-one studies were identified after excluding those that performed a clinical intervention before stool collection. In the early microbiota of children who later developed allergies, lower bacterial diversity was observed, with a predominance of Firmicutes; a higher count of Bacteroidaceae; a higher prevalence of the anaerobic bacteria Bacteroides fragilis, Escherichia coli, Clostridium difficile, Bifidobacterium catenulatum, Bifidobacterium bifidum, and Bifidobacterium longum; and a lower prevalence of Bifidobacterium adolescentis, B. bifidum, and Lactobacillus. In the microbiota of allergic children whose intestinal microbiota was assessed at the onset of allergic symptoms, there was a higher count of Bacteroides; a lower count of Akkermansia muciniphila, Faecalibacterium prausnitzii, and Clostridium; a higher prevalence of B. adolescentis; a lower prevalence of B. catenulatum and Staphylococcus aureus; and a lower bacterial diversity.
[Mh] Termos MeSH primário: Bacteroidaceae
Firmicutes
Microbioma Gastrointestinal
Hipersensibilidade/microbiologia
Intestinos/microbiologia
[Mh] Termos MeSH secundário: Animais
Biodiversidade
Criança
Seres Humanos
Hipersensibilidade/dietoterapia
Intestinos/imunologia
Probióticos/uso terapêutico
[Pt] Tipo de publicação:JOURNAL ARTICLE; REVIEW
[Em] Mês de entrada:1612
[Cu] Atualização por classe:161230
[Lr] Data última revisão:
161230
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150520
[St] Status:MEDLINE


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[PMID]:26277409
[Au] Autor:Megson ZA; Pittenauer E; Duda KA; Engel R; Ortmayr K; Koellensperger G; Mach L; Allmaier G; Holst O; Messner P; Schäffer C
[Ad] Endereço:Department of NanoBiotechnology, NanoGlycobiology unit, Universität für Bodenkultur Wien, Muthgasse 11, 1190 Vienna, Austria.
[Ti] Título:Inositol-phosphodihydroceramides in the periodontal pathogen Tannerella forsythia: Structural analysis and incorporation of exogenous myo-inositol.
[So] Source:Biochim Biophys Acta;1851(11):1417-27, 2015 Nov.
[Is] ISSN:0006-3002
[Cp] País de publicação:Netherlands
[La] Idioma:eng
[Ab] Resumo:BACKGROUND: Unique phosphodihydroceramides containing phosphoethanolamine and glycerol have been previously described in Porphyromonas gingivalis. Importantly, they were shown to possess pro-inflammatory properties. Other common human bacteria were screened for the presence of these lipids, and they were found, amongst others, in the oral pathogen Tannerella forsythia. To date, no detailed study into the lipids of this organism has been performed. METHODS: Lipids were extracted, separated and purified by HPTLC, and analyzed using GC-MS, ESI-MS and NMR. Of special interest was how T. forsythia acquires the metabolic precursors for the lipids studied here. This was assayed by radioactive and stable isotope incorporation using carbon-14 and deuterium labeled myo-inositol, added to the growth medium. RESULTS: T. forsythia synthesizes two phosphodihydroceramides (Tf GL1, Tf GL2) which are constituted by phospho-myo-inositol linked to either a 17-, 18-, or 19-carbon sphinganine, N-linked to either a branched 17:0(3-OH) or a linear 16:0(3-OH) fatty acid which, in Tf GL2, is, in turn, ester-substituted with a branched 15:0 fatty acid. T. forsythia lacks the enzymatic machinery required for myo-inositol synthesis but was found to internalize inositol from the medium for the synthesis of both Tf GL1 and Tf GL2. CONCLUSION: The study describes two novel glycolipids in T. forsythia which could be essential in this organism. Their synthesis could be reliant on an external source of myo-inositol. GENERAL SIGNIFICANCE: The effects of these unique lipids on the immune system and their role in bacterial virulence could be relevant in the search for new drug targets.
[Mh] Termos MeSH primário: Bacteroidaceae/metabolismo
Ceramidas/análise
Etanolaminas/análise
Inositol/metabolismo
[Mh] Termos MeSH secundário: Bacteroidaceae/química
Radioisótopos de Carbono
Ceramidas/biossíntese
Ceramidas/química
Cromatografia Líquida de Alta Pressão
Deutério
Etanolaminas/química
Etanolaminas/metabolismo
Glicerol/análise
Glicerol/química
Marcação por Isótopo
Extração Líquido-Líquido
Espectroscopia de Ressonância Magnética
Esfingosina/análogos & derivados
Esfingosina/química
Esfingosina/metabolismo
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Carbon Radioisotopes); 0 (Ceramides); 0 (Ethanolamines); 4L6452S749 (Inositol); 78A2BX7AEU (phosphorylethanolamine); AR09D82C7G (Deuterium); NGZ37HRE42 (Sphingosine); OWA98U788S (safingol); PDC6A3C0OX (Glycerol)
[Em] Mês de entrada:1512
[Cu] Atualização por classe:170922
[Lr] Data última revisão:
170922
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150817
[St] Status:MEDLINE


  9 / 427 MEDLINE  
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[PMID]:25907974
[Au] Autor:Bohlmann L; Chang CW; Beacham I; von Itzstein M
[Ad] Endereço:Institute for Glycomics, Gold Coast Campus, Griffith University, Queensland, 4222 (Australia).
[Ti] Título:Exploring bacterial heparinase II activities with defined substrates.
[So] Source:Chembiochem;16(8):1205-11, 2015 May 26.
[Is] ISSN:1439-7633
[Cp] País de publicação:Germany
[La] Idioma:eng
[Ab] Resumo:Bacterial heparinases that cleave heparan sulfate (HS) and heparin are widely used to generate low-molecular-weight heparins (LMWHs) and to structurally and functionally characterise heparin and HS biomolecules. We provide novel insights into the substrate specificity of heparinase II from two different bacteria: Pedobacter heparinus (formerly Flavobacterium heparinum) and Bacteroides eggerthii. The activity towards various well-defined HS oligosaccharides was investigated by (1) H NMR spectroscopy; this revealed distinct specificities for the two heparinases. Heparinase II from P. heparinus appears to be more active and displays a broader substrate specificity than B. eggerthii heparinase II. Furthermore, HS di- and tetrasaccharides inhibited B. eggerthii heparinase II activity. A better understanding of heparinase substrate specificity will contribute to the production of homogenous LMWHs, provide better characterisation of heparin and HS and assist therapeutic applications.
[Mh] Termos MeSH primário: Bacteroidaceae/enzimologia
Pedobacter/enzimologia
Polissacarídeo-Liase/metabolismo
[Mh] Termos MeSH secundário: Antitrombinas/metabolismo
Sítios de Ligação
Inibidores Enzimáticos/química
Inibidores Enzimáticos/farmacologia
Heparitina Sulfato/química
Heparitina Sulfato/metabolismo
Polissacarídeo-Liase/antagonistas & inibidores
Especificidade por Substrato
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (Antithrombins); 0 (Enzyme Inhibitors); 9050-30-0 (Heparitin Sulfate); EC 4.2.2.- (Polysaccharide-Lyases); EC 4.2.2.- (heparinase II)
[Em] Mês de entrada:1602
[Cu] Atualização por classe:150515
[Lr] Data última revisão:
150515
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150425
[St] Status:MEDLINE
[do] DOI:10.1002/cbic.201500081


  10 / 427 MEDLINE  
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[PMID]:25819443
[Au] Autor:Pérez-Salcedo L; Laguna E; Sánchez MC; Marín MJ; O'Connor A; González I; Sanz M; Herrera D
[Ad] Endereço:Research Laboratory, Faculty of Odontology, University Complutense, Plaza de Ramón y Cajal, 28040 Madrid, Spain.
[Ti] Título:Molecular identification of black-pigmented bacteria from subgingival samples of cats suffering from periodontal disease.
[So] Source:J Small Anim Pract;56(4):270-5, 2015 Apr.
[Is] ISSN:1748-5827
[Cp] País de publicação:England
[La] Idioma:eng
[Ab] Resumo:OBJECTIVES: To characterise the black-pigmented bacterial species found in the subgingival samples of cats with periodontal disease using molecular-based microbiological techniques. METHODS: Sixty-five subgingival samples obtained from 50 cats with periodontal disease were analysed by polymerase chain reaction amplified ribosomal DNA restriction analysis and cloning and sequencing of the 16S rRNA genes. RESULTS: Among the 65 subgingival samples, eight phylogenetic profiles were obtained, of which the most prevalent species were: Porphyromonas gulae (40%), P. gingivalis/P. gulae (36 · 9%), P. gulae/Porphyromonas sp. UQD 406 (9 · 2%), Odoribacter denticanis (6 · 2%), P. gulae/Porphyromonas sp. UQD 348 (1 · 5%) and P. circumdentaria (1 · 5%). When compared with the species resulting from biochemical diagnosis, the identification of P. gulae was congruent in 70% of the cases, while colonies identified as P. intermedia-like corresponded in 80% of cases to P. gulae. CLINICAL SIGNIFICANCE: The use of molecular-based microbiological diagnostic techniques resulted in a predominance of Porphyromonas spp. in the subgingival plaque of cats suffering from periodontal disease. Further characterisation of these bacteria identified P. gulae, O. denticanis and P. circumdentaria. The more frequently detected phylogenetic profiles corresponded to P. gingivalis and P. gulae.
[Mh] Termos MeSH primário: Infecções por Bacteroidaceae/veterinária
Doenças do Gato/microbiologia
Periodontite/veterinária
Porphyromonas/isolamento & purificação
[Mh] Termos MeSH secundário: Animais
Carga Bacteriana
Bacteroidaceae/classificação
Bacteroidaceae/genética
Bacteroidaceae/isolamento & purificação
Infecções por Bacteroidaceae/microbiologia
Gatos
Feminino
Masculino
Periodontite/microbiologia
Reação em Cadeia da Polimerase/veterinária
Porphyromonas/classificação
Porphyromonas/genética
RNA Ribossômico 16S/análise
[Pt] Tipo de publicação:JOURNAL ARTICLE; RESEARCH SUPPORT, NON-U.S. GOV'T
[Nm] Nome de substância:
0 (RNA, Ribosomal, 16S)
[Em] Mês de entrada:1603
[Cu] Atualização por classe:150330
[Lr] Data última revisão:
150330
[Sb] Subgrupo de revista:IM
[Da] Data de entrada para processamento:150331
[St] Status:MEDLINE
[do] DOI:10.1111/jsap.12319



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